U.S. patent application number 16/573538 was filed with the patent office on 2020-01-02 for lepidopteran-active cry1da1 amino acid sequence variant proteins. This patent application is currently assigned to Monsanto Technology LLC. The applicant listed for this patent is Monsanto Technology LLC. Invention is credited to James A. Baum, Thomas Cerruti, Stanislaw Flasinski, Xiaoran Fu, Arlene R. Howe, Sara Ann Salvador.
Application Number | 20200002719 16/573538 |
Document ID | / |
Family ID | 54477236 |
Filed Date | 2020-01-02 |
United States Patent Application | 20200002719 |
Kind Code | A1 |
Baum; James A. ; et al. | January 2, 2020 |
Engineered Cry1Da amino acid sequences are provided that exhibit improved Lepidopteran insecticidal activity and an enhanced Lepidopteran spectrum compared to the naturally occurring Cry1Da protein toxin. Polynucleotide sequences intended for use in expression of the improved proteins in plants are also provided. Particular embodiments provide compositions containing insect inhibitory amounts of the engineered proteins, as well as recombinant plants, plant parts, and seeds containing polynucleotide constructs encoding one or more of the improved engineered proteins.
Inventors: | Baum; James A.; (Webster Groves, MO) ; Cerruti; Thomas; (Newton, MA) ; Flasinski; Stanislaw; (Chesterfield, MO) ; Fu; Xiaoran; (Belmont, MA) ; Howe; Arlene R.; (Chesterfield, MO) ; Salvador; Sara Ann; (Wildwood, MO) | ||||||||||
Applicant: |
|
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Assignee: | Monsanto Technology LLC St. Louis MO |
||||||||||
Family ID: | 54477236 | ||||||||||
Appl. No.: | 16/573538 | ||||||||||
Filed: | September 17, 2019 |
Application Number | Filing Date | Patent Number | ||
---|---|---|---|---|
16047187 | Jul 27, 2018 | 10457958 | ||
16573538 | ||||
14884432 | Oct 15, 2015 | 10059959 | ||
16047187 | ||||
62064994 | Oct 16, 2014 | |||
62065017 | Oct 17, 2014 | |||
Current U.S. Class: | 1/1 |
Current CPC Class: | A01N 47/08 20130101; Y02A 40/162 20180101; Y02A 40/146 20180101; C12N 15/8286 20130101; C07K 14/325 20130101; A01N 63/10 20200101 |
International Class: | C12N 15/82 20060101 C12N015/82; C07K 14/325 20060101 C07K014/325; A01N 47/08 20060101 A01N047/08; A01N 63/02 20060101 A01N063/02 |
Sequence CWU 1
1
4413498DNABacillus thuringiensismisc_feature(1)..(3498)Nucleotide
sequence used for expression in a bacterial cell encoding Cry1Da1.
1atggaaataa ataatcaaaa ccaatgtgtg ccttacaatt gtttaagtaa tcctaaggag
60ataatattag gcgaggaaag gctagaaaca gggaatactg tagcagacat ttcattaggg
120cttattaatt ttctatattc taattttgta ccaggaggag gatttatagt
aggtttacta 180gaattaatat ggggatttat agggccttcg caatgggata
tttttttagc tcaaattgag 240caattgatta gtcaaagaat agaagaattt
gctaggaatc aggcaatttc aagattggag 300gggctaagca atctttataa
ggtctatgtt agagcgttta gcgactggga gaaagatcct 360actaatcctg
ctttaaggga agaaatgcgt atacaattta atgacatgaa tagtgctctc
420ataacggcta ttccactttt tagagttcaa aattatgaag ttgctctttt
atctgtatat 480gttcaagccg caaacttaca tttatctatt ttaagggatg
tttcagtttt cggagaaaga 540tggggatatg atacagcgac tatcaataat
cgctatagtg atctgactag ccttattcat 600gtttatacta accattgtgt
ggatacgtat aatcagggat taaggcgttt ggaaggtcgt 660tttcttagcg
attggattgt atataatcgt ttccggagac aattgacaat ttcagtatta
720gatattgttg cgttttttcc aaattatgat attagaacat atccaattca
aacagctact 780cagctaacga gggaagtcta tctggattta ccttttatta
atgaaaatct ttctcctgca 840gcaagctatc caaccttttc agctgctgaa
agtgctataa ttagaagtcc tcatttagta 900gactttttaa atagctttac
catttataca gatagtctgg cacgttatgc atattgggga 960gggcacttgg
taaattcttt ccgcacagga accactacta atttgataag atccccttta
1020tatggaaggg aaggaaatac agagcgcccc gtaactatta ccgcatcacc
tagcgtacca 1080atatttagaa cactttcata tattacaggc cttgacaatt
caaatcctgt agctggaatc 1140gagggagtgg aattccaaaa tactataagt
agaagtatct atcgtaaaag cggtccaata 1200gattctttta gtgaattacc
acctcaagat gccagcgtat ctcctgcaat tgggtatagt 1260caccgtttat
gccatgcaac atttttagaa cggattagtg gaccaagaat agcaggcacc
1320gtattttctt ggacacaccg tagtgccagc cctactaacg aagtaagtcc
atctagaatt 1380acacaaattc catgggtaaa ggcgcatact cttgcatctg
gtgcctccgt cattaaaggt 1440cctggattta caggtggaga tattctgact
aggaatagta tgggcgagct ggggacctta 1500cgagtaacct tcacaggaag
attaccacaa agttattata tacgtttccg ttatgcttcg 1560gtagcaaata
ggagtggtac atttagatat tcacagccac cttcgtatgg aatttcattt
1620ccaaaaacta tggacgcagg tgaaccacta acatctcgtt cgttcgctca
tacaacactc 1680ttcactccaa taaccttttc acgagctcaa gaagaatttg
atctatacat ccaatcgggt 1740gtttatatag atcgaattga atttataccg
gttactgcaa catttgaggc agaatatgat 1800ttagaaagag cgcaaaaggt
ggtgaatgcc ctgtttacgt ctacaaacca actagggcta 1860aaaacagatg
tgacggatta tcatattgat caggtatcca atctagttgc gtgtttatcg
1920gatgaatttt gtctggatga aaagagagaa ttgtccgaga aagttaaaca
tgcaaagcga 1980ctcagtgatg agcggaattt acttcaagat ccaaacttca
gagggatcaa taggcaacca 2040gaccgtggct ggagaggaag tacggatatt
actatccaag gaggagatga cgtattcaaa 2100gagaattacg ttacgctacc
gggtaccttt gatgagtgct atccaacgta tttatatcaa 2160aaaatagatg
agtcgaaatt aaaagcctat acccgttatc aattaagagg gtatatcgaa
2220gatagtcaag acttagaaat ctatttaatt cgttacaatg caaaacacga
aatagtaaat 2280gtaccaggta caggaagttt atggcctctt tctgtagaaa
atcaaattgg accttgtgga 2340gaaccgaatc gatgcgcgcc acaccttgaa
tggaatcctg atttacactg ttcctgcaga 2400gacggggaaa aatgtgcaca
tcattctcat catttctctt tggacattga tgttggatgt 2460acagacttaa
atgaggactt aggtgtatgg gtgatattca agattaagac gcaagatggc
2520cacgcacgac tagggaatct agagtttctc gaagagaaac cattattagg
agaagcacta 2580gctcgtgtga aaagagcgga gaaaaaatgg agagacaaac
gcgaaacatt acaattggaa 2640acaactatcg tttataaaga ggcaaaagaa
tctgtagatg ctttatttgt aaactctcaa 2700tatgatagat tacaagcgga
tacgaacatc gcgatgattc atgcggcaga taaacgcgtt 2760catagaattc
gagaagcgta tctgccggag ctgtctgtga ttccgggtgt caatgcggct
2820atttttgaag aattagaaga gcgtattttc actgcatttt ccctatatga
tgcgagaaat 2880attattaaaa atggcgattt caataatggc ttattatgct
ggaacgtgaa agggcatgta 2940gaggtagaag aacaaaacaa tcaccgttca
gtcctggtta tcccagaatg ggaggcagaa 3000gtgtcacaag aggttcgtgt
ctgtccaggt cgtggctata tccttcgtgt tacagcgtac 3060aaagagggat
atggagaagg ttgcgtaacg atccatgaga tcgagaacaa tacagacgaa
3120ctgaaattca acaactgtgt agaagaggaa gtatatccaa acaacacggt
aacgtgtatt 3180aattatactg cgactcaaga agaatatgag ggtacgtaca
cttctcgtaa tcgaggatat 3240gacgaagcct atggtaataa cccttccgta
ccagctgatt atgcgtcagt ctatgaagaa 3300aaatcgtata cagatagacg
aagagagaat ccttgtgaat ctaacagagg atatggagat 3360tacacaccac
taccagctgg ttatgtaaca aaggaattag agtacttccc agagaccgat
3420aaggtatgga ttgagattgg agaaacagaa ggaacattca tcgtggacag
cgtggaatta 3480ctccttatgg aggaatag 349821165PRTBacillus
thuringiensisMISC_FEATURE(1)..(1165)Amino acid sequence of the
protein Cry1Da1. 2Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr
Asn Cys Leu Ser1 5 10 15Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg
Leu Glu Thr Gly Asn 20 25 30Thr Val Ala Asp Ile Ser Leu Gly Leu Ile
Asn Phe Leu Tyr Ser Asn 35 40 45Phe Val Pro Gly Gly Gly Phe Ile Val
Gly Leu Leu Glu Leu Ile Trp 50 55 60Gly Phe Ile Gly Pro Ser Gln Trp
Asp Ile Phe Leu Ala Gln Ile Glu65 70 75 80Gln Leu Ile Ser Gln Arg
Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 85 90 95Ser Arg Leu Glu Gly
Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 100 105 110Phe Ser Asp
Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 115 120 125Met
Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 130 135
140Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val
Tyr145 150 155 160Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg
Asp Val Ser Val 165 170 175Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala
Thr Ile Asn Asn Arg Tyr 180 185 190Ser Asp Leu Thr Ser Leu Ile His
Val Tyr Thr Asn His Cys Val Asp 195 200 205Thr Tyr Asn Gln Gly Leu
Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 210 215 220Trp Ile Val Tyr
Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu225 230 235 240Asp
Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 245 250
255Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe
260 265 270Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe
Ser Ala 275 280 285Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val
Asp Phe Leu Asn 290 295 300Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala
Arg Tyr Ala Tyr Trp Gly305 310 315 320Gly His Leu Val Asn Ser Phe
Arg Thr Gly Thr Thr Thr Asn Leu Ile 325 330 335Arg Ser Pro Leu Tyr
Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 340 345 350Ile Thr Ala
Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Ile 355 360 365Thr
Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 370 375
380Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro
Ile385 390 395 400Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser
Val Ser Pro Ala 405 410 415Ile Gly Tyr Ser His Arg Leu Cys His Ala
Thr Phe Leu Glu Arg Ile 420 425 430Ser Gly Pro Arg Ile Ala Gly Thr
Val Phe Ser Trp Thr His Arg Ser 435 440 445Ala Ser Pro Thr Asn Glu
Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 450 455 460Trp Val Lys Ala
His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly465 470 475 480Pro
Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 485 490
495Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr
500 505 510Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly
Thr Phe 515 520 525Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe
Pro Lys Thr Met 530 535 540Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser
Phe Ala His Thr Thr Leu545 550 555 560Phe Thr Pro Ile Thr Phe Ser
Arg Ala Gln Glu Glu Phe Asp Leu Tyr 565 570 575Ile Gln Ser Gly Val
Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 580 585 590Ala Thr Phe
Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val Val 595 600 605Asn
Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp Val 610 615
620Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu
Ser625 630 635 640Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser
Glu Lys Val Lys 645 650 655His Ala Lys Arg Leu Ser Asp Glu Arg Asn
Leu Leu Gln Asp Pro Asn 660 665 670Phe Arg Gly Ile Asn Arg Gln Pro
Asp Arg Gly Trp Arg Gly Ser Thr 675 680 685Asp Ile Thr Ile Gln Gly
Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 690 695 700Thr Leu Pro Gly
Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln705 710 715 720Lys
Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 725 730
735Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr
740 745 750Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser
Leu Trp 755 760 765Pro Leu Ser Val Glu Asn Gln Ile Gly Pro Cys Gly
Glu Pro Asn Arg 770 775 780Cys Ala Pro His Leu Glu Trp Asn Pro Asp
Leu His Cys Ser Cys Arg785 790 795 800Asp Gly Glu Lys Cys Ala His
His Ser His His Phe Ser Leu Asp Ile 805 810 815Asp Val Gly Cys Thr
Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile 820 825 830Phe Lys Ile
Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu 835 840 845Phe
Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val Lys 850 855
860Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln Leu
Glu865 870 875 880Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu Ser Val
Asp Ala Leu Phe 885 890 895Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala
Asp Thr Asn Ile Ala Met 900 905 910Ile His Ala Ala Asp Lys Arg Val
His Arg Ile Arg Glu Ala Tyr Leu 915 920 925Pro Glu Leu Ser Val Ile
Pro Gly Val Asn Ala Ala Ile Phe Glu Glu 930 935 940Leu Glu Glu Arg
Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn945 950 955 960Ile
Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn Val 965 970
975Lys Gly His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser Val Leu
980 985 990Val Ile Pro Glu Trp Glu Ala Glu Val Ser Gln Glu Val Arg
Val Cys 995 1000 1005Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr Ala
Tyr Lys Glu Gly 1010 1015 1020Tyr Gly Glu Gly Cys Val Thr Ile His
Glu Ile Glu Asn Asn Thr 1025 1030 1035Asp Glu Leu Lys Phe Asn Asn
Cys Val Glu Glu Glu Val Tyr Pro 1040 1045 1050Asn Asn Thr Val Thr
Cys Ile Asn Tyr Thr Ala Thr Gln Glu Glu 1055 1060 1065Tyr Glu Gly
Thr Tyr Thr Ser Arg Asn Arg Gly Tyr Asp Glu Ala 1070 1075 1080Tyr
Gly Asn Asn Pro Ser Val Pro Ala Asp Tyr Ala Ser Val Tyr 1085 1090
1095Glu Glu Lys Ser Tyr Thr Asp Arg Arg Arg Glu Asn Pro Cys Glu
1100 1105 1110Ser Asn Arg Gly Tyr Gly Asp Tyr Thr Pro Leu Pro Ala
Gly Tyr 1115 1120 1125Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu Thr
Asp Lys Val Trp 1130 1135 1140Ile Glu Ile Gly Glu Thr Glu Gly Thr
Phe Ile Val Asp Ser Val 1145 1150 1155Glu Leu Leu Leu Met Glu Glu
1160 116533498DNAArtificial SequenceNucleotide sequence used for
expression in a bacterial cell encoding Cry1Da1_3. 3atggaaataa
ataatcaaaa ccaatgtgtg ccttacaatt gtttaagtaa tcctaaggag 60ataatattag
gcgaggaaag gctagaaaca gggaatactg tagcagacat ttcattaggg
120cttattaatt ttctatattc taattttgta ccaggaggag gatttatagt
aggtttacta 180gaattaatat ggggatttat agggccttcg caatgggata
tttttttagc tcaaattgag 240caattgatta gtcaaagaat agaagaattt
gctaggaatc aggcaatttc aagattggag 300gggctaagca atctttataa
ggtctatgtt agagcgttta gcgactggga gaaagatcct 360actaatcctg
ctttaaggga agaaatgcgt atacaattta atgacatgaa tagtgctctc
420ataacggcta ttccactttt tagagttcaa aattatgaag ttgctctttt
atctgtatat 480gttcaagccg caaacttaca tttatctatt ttaagggatg
tttcagtttt cggagaaaga 540tggggatatg atacagcgac tatcaataat
cgctatagtg atctgactag ccttattcat 600gtttatacta accattgtgt
ggatacgtat aatcagggat taaggcgttt ggaaggtcgt 660tttcttagcg
attggattgt atataatcgt ttccggagac aattgacaat ttcagtatta
720gatattgttg cgttttttcc aaattatgat attagaacat atccaattca
aacagctact 780cagctaacga gggaagtcta tctggattta ccttttatta
atgaaaatct ttctcctgca 840gcaagctatc caaccttttc agctgctgaa
agtgctataa ttagaagtcc tcatttagta 900gactttttaa atagctttac
catttataca gatagtctgg cacgtagtgc atattgggga 960gggcacttgg
taaattcttt ccgcacagga accactacta atttgataag atccccttta
1020tatggaaggg aaggaaatac agagcgcccc gtaactatta ccgcatcacc
tagcgtacca 1080atatttagaa cactttcata tattacaggc cttgacaatt
caaatcctgt agctggaatc 1140gagggagtgg aattccaaaa tactataagt
agaagtatct atcgtaaaag cggtccaata 1200gattctttta gtgaattacc
acctcaagat gccagcgtat ctcctgcaat tgggtatagt 1260caccgtttat
gccatgcaac atttttagaa cggattagtg gaccaagaat agcaggcacc
1320gtattttctt ggacacaccg tagtgccagc cctactaacg aagtaagtcc
atctagaatt 1380acacaaattc catgggtaaa ggcgcatact cttgcatctg
gtgcctccgt cattaaaggt 1440cctggattta caggtggaga tattctgact
aggaatagta tgggcgagct ggggacctta 1500cgagtaacct tcacaggaag
attaccacaa agttattata tacgtttccg ttatgcttcg 1560gtagcaaata
ggagtggtac atttagatat tcacagccac cttcgtatgg aatttcattt
1620ccaaaaacta tggacgcagg tgaaccacta acatctcgtt cgttcgctca
tacaacactc 1680ttcactccaa taaccttttc acgagctcaa gaagaatttg
atctatacat ccaatcgggt 1740gtttatatag atcgaattga atttataccg
gttactgcaa catttgaggc agaatatgat 1800ttagaaagag cgcaaaaggt
ggtgaatgcc ctgtttacgt ctacaaacca actagggcta 1860aaaacagatg
tgacggatta tcatattgat caggtatcca atctagttgc gtgtttatcg
1920gatgaatttt gtctggatga aaagagagaa ttgtccgaga aagttaaaca
tgcaaagcga 1980ctcagtgatg agcggaattt acttcaagat ccaaacttca
gagggatcaa taggcaacca 2040gaccgtggct ggagaggaag tacggatatt
actatccaag gaggagatga cgtattcaaa 2100gagaattacg ttacgctacc
gggtaccttt gatgagtgct atccaacgta tttatatcaa 2160aaaatagatg
agtcgaaatt aaaagcctat acccgttatc aattaagagg gtatatcgaa
2220gatagtcaag acttagaaat ctatttaatt cgttacaatg caaaacacga
aatagtaaat 2280gtaccaggta caggaagttt atggcctctt tctgtagaaa
atcaaattgg accttgtgga 2340gaaccgaatc gatgcgcgcc acaccttgaa
tggaatcctg atttacactg ttcctgcaga 2400gacggggaaa aatgtgcaca
tcattctcat catttctctt tggacattga tgttggatgt 2460acagacttaa
atgaggactt aggtgtatgg gtgatattca agattaagac gcaagatggc
2520cacgcacgac tagggaatct agagtttctc gaagagaaac cattattagg
agaagcacta 2580gctcgtgtga aaagagcgga gaaaaaatgg agagacaaac
gcgaaacatt acaattggaa 2640acaactatcg tttataaaga ggcaaaagaa
tctgtagatg ctttatttgt aaactctcaa 2700tatgatagat tacaagcgga
tacgaacatc gcgatgattc atgcggcaga taaacgcgtt 2760catagaattc
gagaagcgta tctgccggag ctgtctgtga ttccgggtgt caatgcggct
2820atttttgaag aattagaaga gcgtattttc actgcatttt ccctatatga
tgcgagaaat 2880attattaaaa atggcgattt caataatggc ttattatgct
ggaacgtgaa agggcatgta 2940gaggtagaag aacaaaacaa tcaccgttca
gtcctggtta tcccagaatg ggaggcagaa 3000gtgtcacaag aggttcgtgt
ctgtccaggt cgtggctata tccttcgtgt tacagcgtac 3060aaagagggat
atggagaagg ttgcgtaacg atccatgaga tcgagaacaa tacagacgaa
3120ctgaaattca acaactgtgt agaagaggaa gtatatccaa acaacacggt
aacgtgtatt 3180aattatactg cgactcaaga agaatatgag ggtacgtaca
cttctcgtaa tcgaggatat 3240gacgaagcct atggtaataa cccttccgta
ccagctgatt atgcgtcagt ctatgaagaa 3300aaatcgtata cagatagacg
aagagagaat ccttgtgaat ctaacagagg atatggagat 3360tacacaccac
taccagctgg ttatgtaaca aaggaattag agtacttccc agagaccgat
3420aaggtatgga ttgagattgg agaaacagaa ggaacattca tcgtggacag
cgtggaatta 3480ctccttatgg aggaatag 349841165PRTArtificial
SequenceAmino acid sequence of the engineered insecticidal protein
Cry1Da1_3. 4Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys
Leu Ser1 5 10 15Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu
Thr Gly Asn 20 25 30Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe
Leu Tyr Ser Asn 35 40 45Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu
Leu Glu Leu Ile Trp 50 55 60Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile
Phe Leu Ala Gln Ile Glu65 70 75
80Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile
85 90 95Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg
Ala 100 105 110Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu
Arg Glu Glu 115 120 125Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala
Leu Ile Thr Ala Ile 130 135 140Pro Leu Phe Arg Val Gln Asn Tyr Glu
Val Ala Leu Leu Ser Val Tyr145 150 155 160Val Gln Ala Ala Asn Leu
His Leu Ser Ile Leu Arg Asp Val Ser Val 165 170 175Phe Gly Glu Arg
Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 180 185 190Ser Asp
Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 195 200
205Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp
210 215 220Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser
Val Leu225 230 235 240Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile
Arg Thr Tyr Pro Ile 245 250 255Gln Thr Ala Thr Gln Leu Thr Arg Glu
Val Tyr Leu Asp Leu Pro Phe 260 265 270Ile Asn Glu Asn Leu Ser Pro
Ala Ala Ser Tyr Pro Thr Phe Ser Ala 275 280 285Ala Glu Ser Ala Ile
Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 290 295 300Ser Phe Thr
Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp Gly305 310 315
320Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile
325 330 335Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro
Val Thr 340 345 350Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr
Leu Ser Tyr Ile 355 360 365Thr Gly Leu Asp Asn Ser Asn Pro Val Ala
Gly Ile Glu Gly Val Glu 370 375 380Phe Gln Asn Thr Ile Ser Arg Ser
Ile Tyr Arg Lys Ser Gly Pro Ile385 390 395 400Asp Ser Phe Ser Glu
Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 405 410 415Ile Gly Tyr
Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 420 425 430Ser
Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 435 440
445Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro
450 455 460Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile
Lys Gly465 470 475 480Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg
Asn Ser Met Gly Glu 485 490 495Leu Gly Thr Leu Arg Val Thr Phe Thr
Gly Arg Leu Pro Gln Ser Tyr 500 505 510Tyr Ile Arg Phe Arg Tyr Ala
Ser Val Ala Asn Arg Ser Gly Thr Phe 515 520 525Arg Tyr Ser Gln Pro
Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 530 535 540Asp Ala Gly
Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu545 550 555
560Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr
565 570 575Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro
Val Thr 580 585 590Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala
Gln Lys Val Val 595 600 605Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu
Gly Leu Lys Thr Asp Val 610 615 620Thr Asp Tyr His Ile Asp Gln Val
Ser Asn Leu Val Ala Cys Leu Ser625 630 635 640Asp Glu Phe Cys Leu
Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys 645 650 655His Ala Lys
Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 660 665 670Phe
Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser Thr 675 680
685Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val
690 695 700Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu
Tyr Gln705 710 715 720Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr
Arg Tyr Gln Leu Arg 725 730 735Gly Tyr Ile Glu Asp Ser Gln Asp Leu
Glu Ile Tyr Leu Ile Arg Tyr 740 745 750Asn Ala Lys His Glu Ile Val
Asn Val Pro Gly Thr Gly Ser Leu Trp 755 760 765Pro Leu Ser Val Glu
Asn Gln Ile Gly Pro Cys Gly Glu Pro Asn Arg 770 775 780Cys Ala Pro
His Leu Glu Trp Asn Pro Asp Leu His Cys Ser Cys Arg785 790 795
800Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile
805 810 815Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp
Val Ile 820 825 830Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu
Gly Asn Leu Glu 835 840 845Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu
Ala Leu Ala Arg Val Lys 850 855 860Arg Ala Glu Lys Lys Trp Arg Asp
Lys Arg Glu Thr Leu Gln Leu Glu865 870 875 880Thr Thr Ile Val Tyr
Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe 885 890 895Val Asn Ser
Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala Met 900 905 910Ile
His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr Leu 915 920
925Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu
930 935 940Leu Glu Glu Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala
Arg Asn945 950 955 960Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu
Leu Cys Trp Asn Val 965 970 975Lys Gly His Val Glu Val Glu Glu Gln
Asn Asn His Arg Ser Val Leu 980 985 990Val Ile Pro Glu Trp Glu Ala
Glu Val Ser Gln Glu Val Arg Val Cys 995 1000 1005Pro Gly Arg Gly
Tyr Ile Leu Arg Val Thr Ala Tyr Lys Glu Gly 1010 1015 1020Tyr Gly
Glu Gly Cys Val Thr Ile His Glu Ile Glu Asn Asn Thr 1025 1030
1035Asp Glu Leu Lys Phe Asn Asn Cys Val Glu Glu Glu Val Tyr Pro
1040 1045 1050Asn Asn Thr Val Thr Cys Ile Asn Tyr Thr Ala Thr Gln
Glu Glu 1055 1060 1065Tyr Glu Gly Thr Tyr Thr Ser Arg Asn Arg Gly
Tyr Asp Glu Ala 1070 1075 1080Tyr Gly Asn Asn Pro Ser Val Pro Ala
Asp Tyr Ala Ser Val Tyr 1085 1090 1095Glu Glu Lys Ser Tyr Thr Asp
Arg Arg Arg Glu Asn Pro Cys Glu 1100 1105 1110Ser Asn Arg Gly Tyr
Gly Asp Tyr Thr Pro Leu Pro Ala Gly Tyr 1115 1120 1125Val Thr Lys
Glu Leu Glu Tyr Phe Pro Glu Thr Asp Lys Val Trp 1130 1135 1140Ile
Glu Ile Gly Glu Thr Glu Gly Thr Phe Ile Val Asp Ser Val 1145 1150
1155Glu Leu Leu Leu Met Glu Glu 1160 116553498DNAArtificial
SequenceNucleotide sequence used for expression in a bacterial cell
encoding Cry1Da1_4. 5atggaaataa ataatcaaaa ccaatgtgtg ccttacaatt
gtttaagtaa tcctaaggag 60ataatattag gcgaggaaag gctagaaaca gggaatactg
tagcagacat ttcattaggg 120cttattaatt ttctatattc taattttgta
ccaggaggag gatttatagt aggtttacta 180gaattaatat ggggatttat
agggccttcg caatgggata tttttttagc tcaaattgag 240caattgatta
gtcaaagaat agaagaattt gctaggaatc aggcaatttc aagattggag
300gggctaagca atctttataa ggtctatgtt agagcgttta gcgactggga
gaaagatcct 360actaatcctg ctttaaggga agaaatgcgt atacaattta
atgacatgaa tagtgctctc 420ataacggcta ttccactttt tagagttcaa
aattatgaag ttgctctttt atctgtatat 480gttcaagccg caaacttaca
tttatctatt ttaagggatg tttcagtttt cggagaaaga 540tggggatatg
atacagcgac tatcaataat cgctatagtg atctgactag ccttattcat
600gtttatacta accattgtgt ggatacgtat aatcagggat taaggcgttt
ggaaggtcgt 660tttcttagcg attggattgt atataatcgt ttccggagac
aattgacaat ttcagtatta 720gatattgttg cgttttttcc aaattatgat
attagaacat atccaattca aacagctact 780cagctaacga gggaagtcta
tctggattta ccttttatta atgaaaatct ttctcctgca 840gcaagctatc
caaccttttc agctgctgaa agtgctataa ttagaagtcc tcatttagta
900gactttttaa atagctttac catttataca gatagtctgg cacgttatgc
atattgggga 960gggcacttgg taaattcttt ccgcacagga accactacta
atttgataag atccccttta 1020tatggaaggg aaggaaatac agagcgcccc
gtaactatta ccgcatcacc tagcgtacca 1080atatttagaa cactttcata
tattacaggc cttgacaatc gtaatcctgt agctggaatc 1140gagggagtgg
aattccaaaa tactataagt agaagtatct atcgtaaaag cggtccaata
1200gattctttta gtgaattacc acctcaagat gccagcgtat ctcctgcaat
tgggtatagt 1260caccgtttat gccatgcaac atttttagaa cggattagtg
gaccaagaat agcaggcacc 1320gtattttctt ggacacaccg tagtgccagc
cctactaacg aagtaagtcc atctagaatt 1380acacaaattc catgggtaaa
ggcgcatact cttgcatctg gtgcctccgt cattaaaggt 1440cctggattta
caggtggaga tattctgact aggaatagta tgggcgagct ggggacctta
1500cgagtaacct tcacaggaag attaccacaa agttattata tacgtttccg
ttatgcttcg 1560gtagcaaata ggagtggtac atttagatat tcacagccac
cttcgtatgg aatttcattt 1620ccaaaaacta tggacgcagg tgaaccacta
acatctcgtt cgttcgctca tacaacactc 1680ttcactccaa taaccttttc
acgagctcaa gaagaatttg atctatacat ccaatcgggt 1740gtttatatag
atcgaattga atttataccg gttactgcaa catttgaggc agaatatgat
1800ttagaaagag cgcaaaaggt ggtgaatgcc ctgtttacgt ctacaaacca
actagggcta 1860aaaacagatg tgacggatta tcatattgat caggtatcca
atctagttgc gtgtttatcg 1920gatgaatttt gtctggatga aaagagagaa
ttgtccgaga aagttaaaca tgcaaagcga 1980ctcagtgatg agcggaattt
acttcaagat ccaaacttca gagggatcaa taggcaacca 2040gaccgtggct
ggagaggaag tacggatatt actatccaag gaggagatga cgtattcaaa
2100gagaattacg ttacgctacc gggtaccttt gatgagtgct atccaacgta
tttatatcaa 2160aaaatagatg agtcgaaatt aaaagcctat acccgttatc
aattaagagg gtatatcgaa 2220gatagtcaag acttagaaat ctatttaatt
cgttacaatg caaaacacga aatagtaaat 2280gtaccaggta caggaagttt
atggcctctt tctgtagaaa atcaaattgg accttgtgga 2340gaaccgaatc
gatgcgcgcc acaccttgaa tggaatcctg atttacactg ttcctgcaga
2400gacggggaaa aatgtgcaca tcattctcat catttctctt tggacattga
tgttggatgt 2460acagacttaa atgaggactt aggtgtatgg gtgatattca
agattaagac gcaagatggc 2520cacgcacgac tagggaatct agagtttctc
gaagagaaac cattattagg agaagcacta 2580gctcgtgtga aaagagcgga
gaaaaaatgg agagacaaac gcgaaacatt acaattggaa 2640acaactatcg
tttataaaga ggcaaaagaa tctgtagatg ctttatttgt aaactctcaa
2700tatgatagat tacaagcgga tacgaacatc gcgatgattc atgcggcaga
taaacgcgtt 2760catagaattc gagaagcgta tctgccggag ctgtctgtga
ttccgggtgt caatgcggct 2820atttttgaag aattagaaga gcgtattttc
actgcatttt ccctatatga tgcgagaaat 2880attattaaaa atggcgattt
caataatggc ttattatgct ggaacgtgaa agggcatgta 2940gaggtagaag
aacaaaacaa tcaccgttca gtcctggtta tcccagaatg ggaggcagaa
3000gtgtcacaag aggttcgtgt ctgtccaggt cgtggctata tccttcgtgt
tacagcgtac 3060aaagagggat atggagaagg ttgcgtaacg atccatgaga
tcgagaacaa tacagacgaa 3120ctgaaattca acaactgtgt agaagaggaa
gtatatccaa acaacacggt aacgtgtatt 3180aattatactg cgactcaaga
agaatatgag ggtacgtaca cttctcgtaa tcgaggatat 3240gacgaagcct
atggtaataa cccttccgta ccagctgatt atgcgtcagt ctatgaagaa
3300aaatcgtata cagatagacg aagagagaat ccttgtgaat ctaacagagg
atatggagat 3360tacacaccac taccagctgg ttatgtaaca aaggaattag
agtacttccc agagaccgat 3420aaggtatgga ttgagattgg agaaacagaa
ggaacattca tcgtggacag cgtggaatta 3480ctccttatgg aggaatag
349861165PRTArtificial SequenceAmino acid sequence of the
engineered insecticidal protein Cry1Da1_4. 6Met Glu Ile Asn Asn Gln
Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser1 5 10 15Asn Pro Lys Glu Ile
Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 20 25 30Thr Val Ala Asp
Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 35 40 45Phe Val Pro
Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 50 55 60Gly Phe
Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu65 70 75
80Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile
85 90 95Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg
Ala 100 105 110Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu
Arg Glu Glu 115 120 125Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala
Leu Ile Thr Ala Ile 130 135 140Pro Leu Phe Arg Val Gln Asn Tyr Glu
Val Ala Leu Leu Ser Val Tyr145 150 155 160Val Gln Ala Ala Asn Leu
His Leu Ser Ile Leu Arg Asp Val Ser Val 165 170 175Phe Gly Glu Arg
Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 180 185 190Ser Asp
Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 195 200
205Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp
210 215 220Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser
Val Leu225 230 235 240Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile
Arg Thr Tyr Pro Ile 245 250 255Gln Thr Ala Thr Gln Leu Thr Arg Glu
Val Tyr Leu Asp Leu Pro Phe 260 265 270Ile Asn Glu Asn Leu Ser Pro
Ala Ala Ser Tyr Pro Thr Phe Ser Ala 275 280 285Ala Glu Ser Ala Ile
Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 290 295 300Ser Phe Thr
Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Ala Tyr Trp Gly305 310 315
320Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile
325 330 335Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro
Val Thr 340 345 350Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr
Leu Ser Tyr Ile 355 360 365Thr Gly Leu Asp Asn Arg Asn Pro Val Ala
Gly Ile Glu Gly Val Glu 370 375 380Phe Gln Asn Thr Ile Ser Arg Ser
Ile Tyr Arg Lys Ser Gly Pro Ile385 390 395 400Asp Ser Phe Ser Glu
Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 405 410 415Ile Gly Tyr
Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 420 425 430Ser
Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 435 440
445Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro
450 455 460Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile
Lys Gly465 470 475 480Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg
Asn Ser Met Gly Glu 485 490 495Leu Gly Thr Leu Arg Val Thr Phe Thr
Gly Arg Leu Pro Gln Ser Tyr 500 505 510Tyr Ile Arg Phe Arg Tyr Ala
Ser Val Ala Asn Arg Ser Gly Thr Phe 515 520 525Arg Tyr Ser Gln Pro
Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 530 535 540Asp Ala Gly
Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu545 550 555
560Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr
565 570 575Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro
Val Thr 580 585 590Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala
Gln Lys Val Val 595 600 605Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu
Gly Leu Lys Thr Asp Val 610 615 620Thr Asp Tyr His Ile Asp Gln Val
Ser Asn Leu Val Ala Cys Leu Ser625 630 635 640Asp Glu Phe Cys Leu
Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys 645 650 655His Ala Lys
Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 660 665 670Phe
Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser Thr 675 680
685Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val
690 695 700Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu
Tyr Gln705 710 715 720Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr
Arg Tyr Gln Leu Arg 725 730 735Gly Tyr Ile Glu Asp Ser Gln Asp Leu
Glu Ile Tyr Leu Ile Arg Tyr 740 745 750Asn Ala Lys His Glu Ile Val
Asn Val Pro Gly Thr Gly Ser Leu Trp 755 760 765Pro Leu Ser Val Glu
Asn Gln
Ile Gly Pro Cys Gly Glu Pro Asn Arg 770 775 780Cys Ala Pro His Leu
Glu Trp Asn Pro Asp Leu His Cys Ser Cys Arg785 790 795 800Asp Gly
Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile 805 810
815Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile
820 825 830Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn
Leu Glu 835 840 845Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu
Ala Arg Val Lys 850 855 860Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg
Glu Thr Leu Gln Leu Glu865 870 875 880Thr Thr Ile Val Tyr Lys Glu
Ala Lys Glu Ser Val Asp Ala Leu Phe 885 890 895Val Asn Ser Gln Tyr
Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala Met 900 905 910Ile His Ala
Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr Leu 915 920 925Pro
Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu 930 935
940Leu Glu Glu Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg
Asn945 950 955 960Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu
Cys Trp Asn Val 965 970 975Lys Gly His Val Glu Val Glu Glu Gln Asn
Asn His Arg Ser Val Leu 980 985 990Val Ile Pro Glu Trp Glu Ala Glu
Val Ser Gln Glu Val Arg Val Cys 995 1000 1005Pro Gly Arg Gly Tyr
Ile Leu Arg Val Thr Ala Tyr Lys Glu Gly 1010 1015 1020Tyr Gly Glu
Gly Cys Val Thr Ile His Glu Ile Glu Asn Asn Thr 1025 1030 1035Asp
Glu Leu Lys Phe Asn Asn Cys Val Glu Glu Glu Val Tyr Pro 1040 1045
1050Asn Asn Thr Val Thr Cys Ile Asn Tyr Thr Ala Thr Gln Glu Glu
1055 1060 1065Tyr Glu Gly Thr Tyr Thr Ser Arg Asn Arg Gly Tyr Asp
Glu Ala 1070 1075 1080Tyr Gly Asn Asn Pro Ser Val Pro Ala Asp Tyr
Ala Ser Val Tyr 1085 1090 1095Glu Glu Lys Ser Tyr Thr Asp Arg Arg
Arg Glu Asn Pro Cys Glu 1100 1105 1110Ser Asn Arg Gly Tyr Gly Asp
Tyr Thr Pro Leu Pro Ala Gly Tyr 1115 1120 1125Val Thr Lys Glu Leu
Glu Tyr Phe Pro Glu Thr Asp Lys Val Trp 1130 1135 1140Ile Glu Ile
Gly Glu Thr Glu Gly Thr Phe Ile Val Asp Ser Val 1145 1150 1155Glu
Leu Leu Leu Met Glu Glu 1160 116573498DNAArtificial
SequenceNucleotide sequence used for expression in a bacterial cell
encoding Cry1Da1_5. 7atggaaataa ataatcaaaa ccaatgtgtg ccttacaatt
gtttaagtaa tcctaaggag 60ataatattag gcgaggaaag gctagaaaca gggaatactg
tagcagacat ttcattaggg 120cttattaatt ttctatattc taattttgta
ccaggaggag gatttatagt aggtttacta 180gaattaatat ggggatttat
agggccttcg caatgggata tttttttagc tcaaattgag 240caattgatta
gtcaaagaat agaagaattt gctaggaatc aggcaatttc aagattggag
300gggctaagca atctttataa ggtctatgtt agagcgttta gcgactggga
gaaagatcct 360actaatcctg ctttaaggga agaaatgcgt atacaattta
atgacatgaa tagtgctctc 420ataacggcta ttccactttt tagagttcaa
aattatgaag ttgctctttt atctgtatat 480gttcaagccg caaacttaca
tttatctatt ttaagggatg tttcagtttt cggagaaaga 540tggggatatg
atacagcgac tatcaataat cgctatagtg atctgactag ccttattcat
600gtttatacta accattgtgt ggatacgtat aatcagggat taaggcgttt
ggaaggtcgt 660tttcttagcg attggattgt atataatcgt ttccggagac
aattgacaat ttcagtatta 720gatattgttg cgttttttcc aaattatgat
attagaacat atccaattca aacagctact 780cagctaacga gggaagtcta
tctggattta ccttttatta atgaaaatct ttctcctgca 840gcaagctatc
caaccttttc agctgctgaa agtgctataa ttagaagtcc tcatttagta
900gactttttaa atagctttac catttataca gatagtctgg cacgttctgc
atattgggga 960gggcacttgg taaattcttt ccgcacagga accactacta
atttgataag atccccttta 1020tatggaaggg aaggaaatac agagcgcccc
gtaactatta ccgcatcacc tagcgtacca 1080atatttagaa cactttcata
tagaacaggc cttgacaatt caaatcctgt agctggaatc 1140gagggagtgg
aattccaaaa tactataagt agaagtatct atcgtaaaag cggtccaata
1200gattctttta gtgaattacc acctcaagat gccagcgtat ctcctgcaat
tgggtatagt 1260caccgtttat gccatgcaac atttttagaa cggattagtg
gaccaagaat agcaggcacc 1320gtattttctt ggacacaccg tagtgccagc
cctactaacg aagtaagtcc atctagaatt 1380acacaaattc catgggtaaa
ggcgcatact cttgcatctg gtgcctccgt cattaaaggt 1440cctggattta
caggtggaga tattctgact aggaatagta tgggcgagct ggggacctta
1500cgagtaacct tcacaggaag attaccacaa agttattata tacgtttccg
ttatgcttcg 1560gtagcaaata ggagtggtac atttagatat tcacagccac
cttcgtatgg aatttcattt 1620ccaaaaacta tggacgcagg tgaaccacta
acatctcgtt cgttcgctca tacaacactc 1680ttcactccaa taaccttttc
acgagctcaa gaagaatttg atctatacat ccaatcgggt 1740gtttatatag
atcgaattga atttataccg gttactgcaa catttgaggc agaatatgat
1800ttagaaagag cgcaaaaggt ggtgaatgcc ctgtttacgt ctacaaacca
actagggcta 1860aaaacagatg tgacggatta tcatattgat caggtatcca
atctagttgc gtgtttatcg 1920gatgaatttt gtctggatga aaagagagaa
ttgtccgaga aagttaaaca tgcaaagcga 1980ctcagtgatg agcggaattt
acttcaagat ccaaacttca gagggatcaa taggcaacca 2040gaccgtggct
ggagaggaag tacggatatt actatccaag gaggagatga cgtattcaaa
2100gagaattacg ttacgctacc gggtaccttt gatgagtgct atccaacgta
tttatatcaa 2160aaaatagatg agtcgaaatt aaaagcctat acccgttatc
aattaagagg gtatatcgaa 2220gatagtcaag acttagaaat ctatttaatt
cgttacaatg caaaacacga aatagtaaat 2280gtaccaggta caggaagttt
atggcctctt tctgtagaaa atcaaattgg accttgtgga 2340gaaccgaatc
gatgcgcgcc acaccttgaa tggaatcctg atttacactg ttcctgcaga
2400gacggggaaa aatgtgcaca tcattctcat catttctctt tggacattga
tgttggatgt 2460acagacttaa atgaggactt aggtgtatgg gtgatattca
agattaagac gcaagatggc 2520cacgcacgac tagggaatct agagtttctc
gaagagaaac cattattagg agaagcacta 2580gctcgtgtga aaagagcgga
gaaaaaatgg agagacaaac gcgaaacatt acaattggaa 2640acaactatcg
tttataaaga ggcaaaagaa tctgtagatg ctttatttgt aaactctcaa
2700tatgatagat tacaagcgga tacgaacatc gcgatgattc atgcggcaga
taaacgcgtt 2760catagaattc gagaagcgta tctgccggag ctgtctgtga
ttccgggtgt caatgcggct 2820atttttgaag aattagaaga gcgtattttc
actgcatttt ccctatatga tgcgagaaat 2880attattaaaa atggcgattt
caataatggc ttattatgct ggaacgtgaa agggcatgta 2940gaggtagaag
aacaaaacaa tcaccgttca gtcctggtta tcccagaatg ggaggcagaa
3000gtgtcacaag aggttcgtgt ctgtccaggt cgtggctata tccttcgtgt
tacagcgtac 3060aaagagggat atggagaagg ttgcgtaacg atccatgaga
tcgagaacaa tacagacgaa 3120ctgaaattca acaactgtgt agaagaggaa
gtatatccaa acaacacggt aacgtgtatt 3180aattatactg cgactcaaga
agaatatgag ggtacgtaca cttctcgtaa tcgaggatat 3240gacgaagcct
atggtaataa cccttccgta ccagctgatt atgcgtcagt ctatgaagaa
3300aaatcgtata cagatagacg aagagagaat ccttgtgaat ctaacagagg
atatggagat 3360tacacaccac taccagctgg ttatgtaaca aaggaattag
agtacttccc agagaccgat 3420aaggtatgga ttgagattgg agaaacagaa
ggaacattca tcgtggacag cgtggaatta 3480ctccttatgg aggaatag
349881165PRTArtificial SequenceAmino acid sequence of the
engineered insecticidal protein Cry1Da1_5. 8Met Glu Ile Asn Asn Gln
Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser1 5 10 15Asn Pro Lys Glu Ile
Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 20 25 30Thr Val Ala Asp
Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 35 40 45Phe Val Pro
Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 50 55 60Gly Phe
Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu65 70 75
80Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile
85 90 95Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg
Ala 100 105 110Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu
Arg Glu Glu 115 120 125Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala
Leu Ile Thr Ala Ile 130 135 140Pro Leu Phe Arg Val Gln Asn Tyr Glu
Val Ala Leu Leu Ser Val Tyr145 150 155 160Val Gln Ala Ala Asn Leu
His Leu Ser Ile Leu Arg Asp Val Ser Val 165 170 175Phe Gly Glu Arg
Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 180 185 190Ser Asp
Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 195 200
205Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp
210 215 220Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser
Val Leu225 230 235 240Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile
Arg Thr Tyr Pro Ile 245 250 255Gln Thr Ala Thr Gln Leu Thr Arg Glu
Val Tyr Leu Asp Leu Pro Phe 260 265 270Ile Asn Glu Asn Leu Ser Pro
Ala Ala Ser Tyr Pro Thr Phe Ser Ala 275 280 285Ala Glu Ser Ala Ile
Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 290 295 300Ser Phe Thr
Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp Gly305 310 315
320Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile
325 330 335Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro
Val Thr 340 345 350Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr
Leu Ser Tyr Arg 355 360 365Thr Gly Leu Asp Asn Ser Asn Pro Val Ala
Gly Ile Glu Gly Val Glu 370 375 380Phe Gln Asn Thr Ile Ser Arg Ser
Ile Tyr Arg Lys Ser Gly Pro Ile385 390 395 400Asp Ser Phe Ser Glu
Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 405 410 415Ile Gly Tyr
Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 420 425 430Ser
Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 435 440
445Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro
450 455 460Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile
Lys Gly465 470 475 480Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg
Asn Ser Met Gly Glu 485 490 495Leu Gly Thr Leu Arg Val Thr Phe Thr
Gly Arg Leu Pro Gln Ser Tyr 500 505 510Tyr Ile Arg Phe Arg Tyr Ala
Ser Val Ala Asn Arg Ser Gly Thr Phe 515 520 525Arg Tyr Ser Gln Pro
Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 530 535 540Asp Ala Gly
Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu545 550 555
560Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr
565 570 575Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro
Val Thr 580 585 590Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala
Gln Lys Val Val 595 600 605Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu
Gly Leu Lys Thr Asp Val 610 615 620Thr Asp Tyr His Ile Asp Gln Val
Ser Asn Leu Val Ala Cys Leu Ser625 630 635 640Asp Glu Phe Cys Leu
Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys 645 650 655His Ala Lys
Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 660 665 670Phe
Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser Thr 675 680
685Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val
690 695 700Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu
Tyr Gln705 710 715 720Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr
Arg Tyr Gln Leu Arg 725 730 735Gly Tyr Ile Glu Asp Ser Gln Asp Leu
Glu Ile Tyr Leu Ile Arg Tyr 740 745 750Asn Ala Lys His Glu Ile Val
Asn Val Pro Gly Thr Gly Ser Leu Trp 755 760 765Pro Leu Ser Val Glu
Asn Gln Ile Gly Pro Cys Gly Glu Pro Asn Arg 770 775 780Cys Ala Pro
His Leu Glu Trp Asn Pro Asp Leu His Cys Ser Cys Arg785 790 795
800Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile
805 810 815Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp
Val Ile 820 825 830Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu
Gly Asn Leu Glu 835 840 845Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu
Ala Leu Ala Arg Val Lys 850 855 860Arg Ala Glu Lys Lys Trp Arg Asp
Lys Arg Glu Thr Leu Gln Leu Glu865 870 875 880Thr Thr Ile Val Tyr
Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe 885 890 895Val Asn Ser
Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala Met 900 905 910Ile
His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr Leu 915 920
925Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu
930 935 940Leu Glu Glu Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala
Arg Asn945 950 955 960Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu
Leu Cys Trp Asn Val 965 970 975Lys Gly His Val Glu Val Glu Glu Gln
Asn Asn His Arg Ser Val Leu 980 985 990Val Ile Pro Glu Trp Glu Ala
Glu Val Ser Gln Glu Val Arg Val Cys 995 1000 1005Pro Gly Arg Gly
Tyr Ile Leu Arg Val Thr Ala Tyr Lys Glu Gly 1010 1015 1020Tyr Gly
Glu Gly Cys Val Thr Ile His Glu Ile Glu Asn Asn Thr 1025 1030
1035Asp Glu Leu Lys Phe Asn Asn Cys Val Glu Glu Glu Val Tyr Pro
1040 1045 1050Asn Asn Thr Val Thr Cys Ile Asn Tyr Thr Ala Thr Gln
Glu Glu 1055 1060 1065Tyr Glu Gly Thr Tyr Thr Ser Arg Asn Arg Gly
Tyr Asp Glu Ala 1070 1075 1080Tyr Gly Asn Asn Pro Ser Val Pro Ala
Asp Tyr Ala Ser Val Tyr 1085 1090 1095Glu Glu Lys Ser Tyr Thr Asp
Arg Arg Arg Glu Asn Pro Cys Glu 1100 1105 1110Ser Asn Arg Gly Tyr
Gly Asp Tyr Thr Pro Leu Pro Ala Gly Tyr 1115 1120 1125Val Thr Lys
Glu Leu Glu Tyr Phe Pro Glu Thr Asp Lys Val Trp 1130 1135 1140Ile
Glu Ile Gly Glu Thr Glu Gly Thr Phe Ile Val Asp Ser Val 1145 1150
1155Glu Leu Leu Leu Met Glu Glu 1160 116593498DNAArtificial
SequenceNucleotide sequence used for expression in a bacterial cell
encoding Cry1Da1_6. 9atggaaataa ataatcaaaa ccaatgtgtg ccttacaatt
gtttaagtaa tcctaaggag 60ataatattag gcgaggaaag gctagaaaca gggaatactg
tagcagacat ttcattaggg 120cttattaatt ttctatattc taattttgta
ccaggaggag gatttatagt aggtttacta 180gaattaatat ggggatttat
agggccttcg caatgggata tttttttagc tcaaattgag 240caattgatta
gtcaaagaat agaagaattt gctaggaatc aggcaatttc aagattggag
300gggctaagca atctttataa ggtctatgtt agagcgttta gcgactggga
gaaagatcct 360actaatcctg ctttaaggga agaaatgcgt atacaattta
atgacatgaa tagtgctctc 420ataacggcta ttccactttt tagagttcaa
aattatgaag ttgctctttt atctgtatat 480gttcaagccg caaacttaca
tttatctatt ttaagggatg tttcagtttt cggagaaaga 540tggggatatg
atacagcgac tatcaataat cgctatagtg atctgactag ccttattcat
600gtttatacta accattgtgt ggatacgtat aatcagggat taaggcgttt
ggaaggtcgt 660tttcttagcg attggattgt atataatcgt ttccggagac
aattgacaat ttcagtatta 720gatattgttg cgttttttcc aaattatgat
attagaacat atccaattca aacagctact 780cagctaacga gggaagtcta
tctggattta ccttttatta atgaaaatct ttctcctgca 840gcaaaatatc
caaccttttc agctgctgaa agtgctataa ttagaagtcc tcatttagta
900gactttttaa atagctttac catttataca gatagtctgg cacgttctgc
atattgggga 960gggcacttgg taaattcttt ccgcacagga accactacta
atttgataag atccccttta 1020tatggaaggg aaggaaatac agagcgcccc
gtaactatta ccgcatcacc tagcgtacca 1080atatttagaa cactttcata
tccaacaggc cttgacaatt caaatcctgt agctggaatc 1140gagggagtgg
aattccaaaa tactataagt agaagtatct atcgtaaaag cggtccaata
1200gattctttta gtgaattacc acctcaagat gccagcgtat ctcctgcaat
tgggtatagt 1260caccgtttat gccatgcaac atttttagaa cggattagtg
gaccaagaat agcaggcacc 1320gtattttctt ggacacaccg tagtgccagc
cctactaacg aagtaagtcc atctagaatt 1380acacaaattc catgggtaaa
ggcgcatact cttgcatctg gtgcctccgt cattaaaggt 1440cctggattta
caggtggaga tattctgact aggaatagta tgggcgagct ggggacctta
1500cgagtaacct tcacaggaag attaccacaa agttattata tacgtttccg
ttatgcttcg 1560gtagcaaata ggagtggtac atttagatat tcacagccac
cttcgtatgg aatttcattt 1620ccaaaaacta tggacgcagg tgaaccacta
acatctcgtt cgttcgctca tacaacactc 1680ttcactccaa taaccttttc
acgagctcaa gaagaatttg atctatacat ccaatcgggt 1740gtttatatag
atcgaattga atttataccg gttactgcaa catttgaggc agaatatgat
1800ttagaaagag cgcaaaaggt ggtgaatgcc ctgtttacgt ctacaaacca
actagggcta 1860aaaacagatg tgacggatta tcatattgat caggtatcca
atctagttgc gtgtttatcg 1920gatgaatttt gtctggatga aaagagagaa
ttgtccgaga aagttaaaca tgcaaagcga 1980ctcagtgatg agcggaattt
acttcaagat ccaaacttca gagggatcaa taggcaacca 2040gaccgtggct
ggagaggaag tacggatatt actatccaag gaggagatga cgtattcaaa
2100gagaattacg ttacgctacc gggtaccttt gatgagtgct atccaacgta
tttatatcaa 2160aaaatagatg agtcgaaatt aaaagcctat acccgttatc
aattaagagg gtatatcgaa 2220gatagtcaag acttagaaat ctatttaatt
cgttacaatg caaaacacga aatagtaaat 2280gtaccaggta caggaagttt
atggcctctt tctgtagaaa atcaaattgg accttgtgga 2340gaaccgaatc
gatgcgcgcc acaccttgaa tggaatcctg atttacactg ttcctgcaga
2400gacggggaaa aatgtgcaca tcattctcat catttctctt tggacattga
tgttggatgt 2460acagacttaa atgaggactt aggtgtatgg gtgatattca
agattaagac gcaagatggc 2520cacgcacgac tagggaatct agagtttctc
gaagagaaac cattattagg agaagcacta 2580gctcgtgtga aaagagcgga
gaaaaaatgg agagacaaac gcgaaacatt acaattggaa 2640acaactatcg
tttataaaga ggcaaaagaa tctgtagatg ctttatttgt aaactctcaa
2700tatgatagat tacaagcgga tacgaacatc gcgatgattc atgcggcaga
taaacgcgtt 2760catagaattc gagaagcgta tctgccggag ctgtctgtga
ttccgggtgt caatgcggct 2820atttttgaag aattagaaga gcgtattttc
actgcatttt ccctatatga tgcgagaaat 2880attattaaaa atggcgattt
caataatggc ttattatgct ggaacgtgaa agggcatgta 2940gaggtagaag
aacaaaacaa tcaccgttca gtcctggtta tcccagaatg ggaggcagaa
3000gtgtcacaag aggttcgtgt ctgtccaggt cgtggctata tccttcgtgt
tacagcgtac 3060aaagagggat atggagaagg ttgcgtaacg atccatgaga
tcgagaacaa tacagacgaa 3120ctgaaattca acaactgtgt agaagaggaa
gtatatccaa acaacacggt aacgtgtatt 3180aattatactg cgactcaaga
agaatatgag ggtacgtaca cttctcgtaa tcgaggatat 3240gacgaagcct
atggtaataa cccttccgta ccagctgatt atgcgtcagt ctatgaagaa
3300aaatcgtata cagatagacg aagagagaat ccttgtgaat ctaacagagg
atatggagat 3360tacacaccac taccagctgg ttatgtaaca aaggaattag
agtacttccc agagaccgat 3420aaggtatgga ttgagattgg agaaacagaa
ggaacattca tcgtggacag cgtggaatta 3480ctccttatgg aggaatag
3498101165PRTArtificial SequenceAmino acid sequence of the
engineered insecticidal protein Cry1Da1_6. 10Met Glu Ile Asn Asn
Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser1 5 10 15Asn Pro Lys Glu
Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 20 25 30Thr Val Ala
Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 35 40 45Phe Val
Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 50 55 60Gly
Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu65 70 75
80Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile
85 90 95Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg
Ala 100 105 110Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu
Arg Glu Glu 115 120 125Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala
Leu Ile Thr Ala Ile 130 135 140Pro Leu Phe Arg Val Gln Asn Tyr Glu
Val Ala Leu Leu Ser Val Tyr145 150 155 160Val Gln Ala Ala Asn Leu
His Leu Ser Ile Leu Arg Asp Val Ser Val 165 170 175Phe Gly Glu Arg
Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 180 185 190Ser Asp
Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 195 200
205Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp
210 215 220Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser
Val Leu225 230 235 240Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile
Arg Thr Tyr Pro Ile 245 250 255Gln Thr Ala Thr Gln Leu Thr Arg Glu
Val Tyr Leu Asp Leu Pro Phe 260 265 270Ile Asn Glu Asn Leu Ser Pro
Ala Ala Lys Tyr Pro Thr Phe Ser Ala 275 280 285Ala Glu Ser Ala Ile
Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 290 295 300Ser Phe Thr
Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp Gly305 310 315
320Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile
325 330 335Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro
Val Thr 340 345 350Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr
Leu Ser Tyr Pro 355 360 365Thr Gly Leu Asp Asn Ser Asn Pro Val Ala
Gly Ile Glu Gly Val Glu 370 375 380Phe Gln Asn Thr Ile Ser Arg Ser
Ile Tyr Arg Lys Ser Gly Pro Ile385 390 395 400Asp Ser Phe Ser Glu
Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 405 410 415Ile Gly Tyr
Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 420 425 430Ser
Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 435 440
445Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro
450 455 460Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile
Lys Gly465 470 475 480Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg
Asn Ser Met Gly Glu 485 490 495Leu Gly Thr Leu Arg Val Thr Phe Thr
Gly Arg Leu Pro Gln Ser Tyr 500 505 510Tyr Ile Arg Phe Arg Tyr Ala
Ser Val Ala Asn Arg Ser Gly Thr Phe 515 520 525Arg Tyr Ser Gln Pro
Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 530 535 540Asp Ala Gly
Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu545 550 555
560Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr
565 570 575Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro
Val Thr 580 585 590Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala
Gln Lys Val Val 595 600 605Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu
Gly Leu Lys Thr Asp Val 610 615 620Thr Asp Tyr His Ile Asp Gln Val
Ser Asn Leu Val Ala Cys Leu Ser625 630 635 640Asp Glu Phe Cys Leu
Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys 645 650 655His Ala Lys
Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 660 665 670Phe
Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser Thr 675 680
685Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val
690 695 700Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu
Tyr Gln705 710 715 720Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr
Arg Tyr Gln Leu Arg 725 730 735Gly Tyr Ile Glu Asp Ser Gln Asp Leu
Glu Ile Tyr Leu Ile Arg Tyr 740 745 750Asn Ala Lys His Glu Ile Val
Asn Val Pro Gly Thr Gly Ser Leu Trp 755 760 765Pro Leu Ser Val Glu
Asn Gln Ile Gly Pro Cys Gly Glu Pro Asn Arg 770 775 780Cys Ala Pro
His Leu Glu Trp Asn Pro Asp Leu His Cys Ser Cys Arg785 790 795
800Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile
805 810 815Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp
Val Ile 820 825 830Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu
Gly Asn Leu Glu 835 840 845Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu
Ala Leu Ala Arg Val Lys 850 855 860Arg Ala Glu Lys Lys Trp Arg Asp
Lys Arg Glu Thr Leu Gln Leu Glu865 870 875 880Thr Thr Ile Val Tyr
Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe 885 890 895Val Asn Ser
Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala Met 900 905 910Ile
His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr Leu 915 920
925Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu
930 935 940Leu Glu Glu Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala
Arg Asn945 950 955 960Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu
Leu Cys Trp Asn Val 965 970 975Lys Gly His Val Glu Val Glu Glu Gln
Asn Asn His Arg Ser Val Leu 980 985 990Val Ile Pro Glu Trp Glu Ala
Glu Val Ser Gln Glu Val Arg Val Cys 995 1000 1005Pro Gly Arg Gly
Tyr Ile Leu Arg Val Thr Ala Tyr Lys Glu Gly 1010 1015 1020Tyr Gly
Glu Gly Cys Val Thr Ile His Glu Ile Glu Asn Asn Thr 1025 1030
1035Asp Glu Leu Lys Phe Asn Asn Cys Val Glu Glu Glu Val Tyr Pro
1040 1045 1050Asn Asn Thr Val Thr Cys Ile Asn Tyr Thr Ala Thr Gln
Glu Glu 1055 1060 1065Tyr Glu Gly Thr Tyr Thr Ser Arg Asn Arg Gly
Tyr Asp Glu Ala 1070 1075 1080Tyr Gly Asn Asn Pro Ser Val Pro Ala
Asp Tyr Ala Ser Val Tyr 1085 1090 1095Glu Glu Lys Ser Tyr Thr Asp
Arg Arg Arg Glu Asn Pro Cys Glu 1100 1105 1110Ser Asn Arg Gly Tyr
Gly Asp Tyr Thr Pro Leu Pro Ala Gly Tyr 1115 1120 1125Val Thr Lys
Glu Leu Glu Tyr Phe Pro Glu Thr Asp Lys Val Trp 1130 1135 1140Ile
Glu Ile Gly Glu Thr Glu Gly Thr Phe Ile Val Asp Ser Val 1145 1150
1155Glu Leu Leu Leu Met Glu Glu 1160 1165113498DNAArtificial
SequenceNucleotide sequence used for expression in a bacterial cell
encoding Cry1Da1_7. 11atggaaataa ataatcaaaa ccaatgtgtg ccttacaatt
gtttaagtaa tcctaaggag 60ataatattag gcgaggaaag gctagaaaca gggaatactg
tagcagacat ttcattaggg 120cttattaatt ttctatattc taattttgta
ccaggaggag gatttatagt aggtttacta 180gaattaatat ggggatttat
agggccttcg caatgggata tttttttagc tcaaattgag 240caattgatta
gtcaaagaat agaagaattt gctaggaatc aggcaatttc aagattggag
300gggctaagca atctttataa ggtctatgtt agagcgttta gcgactggga
gaaagatcct 360actaatcctg ctttaaggga agaaatgcgt atacaattta
atgacatgaa tagtgctctc 420ataacggcta ttccactttt tagagttcaa
aattatgaag ttgctctttt atctgtatat 480gttcaagccg caaacttaca
tttatctatt ttaagggatg tttcagtttt cggagaaaga 540tggggatatg
atacagcgac tatcaataat cgctatagtg atctgactag ccttattcat
600gtttatacta accattgtgt ggatacgtat aatcagggat taaggcgttt
ggaaggtcgt 660tttcttagcg attggattgt atataatcgt ttccggagac
aattgacaat ttcagtatta 720gatattgttg cgttttttcc aaattatgat
attagaacat atccaattca aacagctact 780cagctaacga gggaagtcta
tctggattta ccttttatta atgaaaatct ttctcctgca 840gcagtatatc
caaccttttc agctgctgaa agtgctataa ttagaagtcc tcatttagta
900gactttttaa atagctttac catttataca gatagtctgg cacgttctgc
atattgggga 960gggcacttgg taaattcttt ccgcacagga accactacta
atttgataag atccccttta 1020tatggaaggg aaggaaatac agagcgcccc
gtaactatta ccgcatcacc tagcgtacca 1080atatttagaa cactttcata
tccaacaggc cttgacaatt caaatcctgt agctggaatc 1140gagggagtgg
aattccaaaa tactataagt agaagtatct atcgtaaaag cggtccaata
1200gattctttta gtgaattacc acctcaagat gccagcgtat ctcctgcaat
tgggtatagt 1260caccgtttat gccatgcaac atttttagaa cggattagtg
gaccaagaat agcaggcacc 1320gtattttctt ggacacaccg tagtgccagc
cctactaacg aagtaagtcc atctagaatt 1380acacaaattc catgggtaaa
ggcgcatact cttgcatctg gtgcctccgt cattaaaggt 1440cctggattta
caggtggaga tattctgact aggaatagta tgggcgagct ggggacctta
1500cgagtaacct tcacaggaag attaccacaa agttattata tacgtttccg
ttatgcttcg 1560gtagcaaata ggagtggtac atttagatat tcacagccac
cttcgtatgg aatttcattt 1620ccaaaaacta tggacgcagg tgaaccacta
acatctcgtt cgttcgctca tacaacactc 1680ttcactccaa taaccttttc
acgagctcaa gaagaatttg atctatacat ccaatcgggt 1740gtttatatag
atcgaattga atttataccg gttactgcaa catttgaggc agaatatgat
1800ttagaaagag cgcaaaaggt ggtgaatgcc ctgtttacgt ctacaaacca
actagggcta 1860aaaacagatg tgacggatta tcatattgat caggtatcca
atctagttgc gtgtttatcg 1920gatgaatttt gtctggatga aaagagagaa
ttgtccgaga aagttaaaca tgcaaagcga 1980ctcagtgatg agcggaattt
acttcaagat ccaaacttca gagggatcaa taggcaacca 2040gaccgtggct
ggagaggaag tacggatatt actatccaag gaggagatga cgtattcaaa
2100gagaattacg ttacgctacc gggtaccttt gatgagtgct atccaacgta
tttatatcaa 2160aaaatagatg agtcgaaatt aaaagcctat acccgttatc
aattaagagg gtatatcgaa 2220gatagtcaag acttagaaat ctatttaatt
cgttacaatg caaaacacga aatagtaaat 2280gtaccaggta caggaagttt
atggcctctt tctgtagaaa atcaaattgg accttgtgga 2340gaaccgaatc
gatgcgcgcc acaccttgaa tggaatcctg atttacactg ttcctgcaga
2400gacggggaaa aatgtgcaca tcattctcat catttctctt tggacattga
tgttggatgt 2460acagacttaa atgaggactt aggtgtatgg gtgatattca
agattaagac gcaagatggc 2520cacgcacgac tagggaatct agagtttctc
gaagagaaac cattattagg agaagcacta 2580gctcgtgtga aaagagcgga
gaaaaaatgg agagacaaac gcgaaacatt acaattggaa 2640acaactatcg
tttataaaga ggcaaaagaa tctgtagatg ctttatttgt aaactctcaa
2700tatgatagat tacaagcgga tacgaacatc gcgatgattc atgcggcaga
taaacgcgtt 2760catagaattc gagaagcgta tctgccggag ctgtctgtga
ttccgggtgt caatgcggct 2820atttttgaag aattagaaga gcgtattttc
actgcatttt ccctatatga tgcgagaaat 2880attattaaaa atggcgattt
caataatggc ttattatgct ggaacgtgaa agggcatgta 2940gaggtagaag
aacaaaacaa tcaccgttca gtcctggtta tcccagaatg ggaggcagaa
3000gtgtcacaag aggttcgtgt ctgtccaggt cgtggctata tccttcgtgt
tacagcgtac 3060aaagagggat atggagaagg ttgcgtaacg atccatgaga
tcgagaacaa tacagacgaa 3120ctgaaattca acaactgtgt agaagaggaa
gtatatccaa acaacacggt aacgtgtatt 3180aattatactg cgactcaaga
agaatatgag ggtacgtaca cttctcgtaa tcgaggatat 3240gacgaagcct
atggtaataa cccttccgta ccagctgatt atgcgtcagt ctatgaagaa
3300aaatcgtata cagatagacg aagagagaat ccttgtgaat ctaacagagg
atatggagat 3360tacacaccac taccagctgg ttatgtaaca aaggaattag
agtacttccc agagaccgat 3420aaggtatgga ttgagattgg agaaacagaa
ggaacattca tcgtggacag cgtggaatta 3480ctccttatgg aggaatag
3498121165PRTArtificial sequenceAmino acid sequence of the
engineered insecticidal protein Cry1Da1_7. 12Met Glu Ile Asn Asn
Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser1 5 10 15Asn Pro Lys Glu
Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 20 25 30Thr Val Ala
Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 35 40 45Phe Val
Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 50 55 60Gly
Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu65 70 75
80Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile
85 90 95Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg
Ala 100 105 110Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu
Arg Glu Glu 115 120 125Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala
Leu Ile Thr Ala Ile 130 135 140Pro Leu Phe Arg Val Gln Asn Tyr Glu
Val Ala Leu Leu Ser Val Tyr145 150 155 160Val Gln Ala Ala Asn Leu
His Leu Ser Ile Leu Arg Asp Val Ser Val 165 170 175Phe Gly Glu Arg
Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 180 185 190Ser Asp
Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 195 200
205Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp
210 215 220Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser
Val Leu225 230 235 240Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile
Arg Thr Tyr Pro Ile 245 250 255Gln Thr Ala Thr Gln Leu Thr Arg Glu
Val Tyr Leu Asp Leu Pro Phe 260 265 270Ile Asn Glu Asn Leu Ser Pro
Ala Ala Val Tyr Pro Thr Phe Ser Ala 275 280 285Ala Glu Ser Ala Ile
Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 290 295 300Ser Phe Thr
Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp Gly305 310 315
320Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile
325 330 335Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro
Val Thr 340 345 350Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr
Leu Ser Tyr Pro 355 360 365Thr Gly Leu Asp Asn Ser Asn Pro Val Ala
Gly Ile Glu Gly Val Glu 370
375 380Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro
Ile385 390 395 400Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser
Val Ser Pro Ala 405 410 415Ile Gly Tyr Ser His Arg Leu Cys His Ala
Thr Phe Leu Glu Arg Ile 420 425 430Ser Gly Pro Arg Ile Ala Gly Thr
Val Phe Ser Trp Thr His Arg Ser 435 440 445Ala Ser Pro Thr Asn Glu
Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 450 455 460Trp Val Lys Ala
His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly465 470 475 480Pro
Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 485 490
495Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr
500 505 510Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly
Thr Phe 515 520 525Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe
Pro Lys Thr Met 530 535 540Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser
Phe Ala His Thr Thr Leu545 550 555 560Phe Thr Pro Ile Thr Phe Ser
Arg Ala Gln Glu Glu Phe Asp Leu Tyr 565 570 575Ile Gln Ser Gly Val
Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 580 585 590Ala Thr Phe
Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val Val 595 600 605Asn
Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp Val 610 615
620Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu
Ser625 630 635 640Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser
Glu Lys Val Lys 645 650 655His Ala Lys Arg Leu Ser Asp Glu Arg Asn
Leu Leu Gln Asp Pro Asn 660 665 670Phe Arg Gly Ile Asn Arg Gln Pro
Asp Arg Gly Trp Arg Gly Ser Thr 675 680 685Asp Ile Thr Ile Gln Gly
Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 690 695 700Thr Leu Pro Gly
Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln705 710 715 720Lys
Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 725 730
735Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr
740 745 750Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser
Leu Trp 755 760 765Pro Leu Ser Val Glu Asn Gln Ile Gly Pro Cys Gly
Glu Pro Asn Arg 770 775 780Cys Ala Pro His Leu Glu Trp Asn Pro Asp
Leu His Cys Ser Cys Arg785 790 795 800Asp Gly Glu Lys Cys Ala His
His Ser His His Phe Ser Leu Asp Ile 805 810 815Asp Val Gly Cys Thr
Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile 820 825 830Phe Lys Ile
Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu 835 840 845Phe
Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val Lys 850 855
860Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln Leu
Glu865 870 875 880Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu Ser Val
Asp Ala Leu Phe 885 890 895Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala
Asp Thr Asn Ile Ala Met 900 905 910Ile His Ala Ala Asp Lys Arg Val
His Arg Ile Arg Glu Ala Tyr Leu 915 920 925Pro Glu Leu Ser Val Ile
Pro Gly Val Asn Ala Ala Ile Phe Glu Glu 930 935 940Leu Glu Glu Arg
Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn945 950 955 960Ile
Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn Val 965 970
975Lys Gly His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser Val Leu
980 985 990Val Ile Pro Glu Trp Glu Ala Glu Val Ser Gln Glu Val Arg
Val Cys 995 1000 1005Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr Ala
Tyr Lys Glu Gly 1010 1015 1020Tyr Gly Glu Gly Cys Val Thr Ile His
Glu Ile Glu Asn Asn Thr 1025 1030 1035Asp Glu Leu Lys Phe Asn Asn
Cys Val Glu Glu Glu Val Tyr Pro 1040 1045 1050Asn Asn Thr Val Thr
Cys Ile Asn Tyr Thr Ala Thr Gln Glu Glu 1055 1060 1065Tyr Glu Gly
Thr Tyr Thr Ser Arg Asn Arg Gly Tyr Asp Glu Ala 1070 1075 1080Tyr
Gly Asn Asn Pro Ser Val Pro Ala Asp Tyr Ala Ser Val Tyr 1085 1090
1095Glu Glu Lys Ser Tyr Thr Asp Arg Arg Arg Glu Asn Pro Cys Glu
1100 1105 1110Ser Asn Arg Gly Tyr Gly Asp Tyr Thr Pro Leu Pro Ala
Gly Tyr 1115 1120 1125Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu Thr
Asp Lys Val Trp 1130 1135 1140Ile Glu Ile Gly Glu Thr Glu Gly Thr
Phe Ile Val Asp Ser Val 1145 1150 1155Glu Leu Leu Leu Met Glu Glu
1160 1165133420DNAArtificial sequenceNucleotide sequence used for
expression in a bacterial cell encoding TIC844. 13atggagatca
acaaccagaa ccagtgcgtc ccgtacaact gcctgagcaa ccctaaggag 60atcatcctgg
gtgaggaacg cctggagacc ggcaacaccg tagccgacat tagcctgggc
120ctcatcaact tcctctacag caacttcgtg cccggcggtg gcttcatcgt
gggcctcctg 180gagcttatct ggggcttcat cggcccgtcc cagtgggaca
tcttcctcgc ccagatcgag 240caactgatca gccagcggat cgaggagttc
gctaggaacc aggccatctc ccgcctggag 300ggactctcca acctctacaa
ggtgtacgtg cgcgcgttca gcgactggga gaaggacccg 360accaacccgg
ccctccgcga ggaaatgcgt atccagttca acgatatgaa ctcggccctc
420atcaccgcca tcccgctctt ccgcgtgcag aactacgagg tggccctcct
gtccgtgtac 480gttcaagccg ccaacctcca cctctccatc ctccgcgacg
tgagcgtgtt cggcgagcgc 540tggggctacg acaccgccac catcaacaac
cgctactccg acctcacctc cctcatccac 600gtttacacca accactgcgt
ggacacgtac aaccagggcc tccgccgcct ggagggccgc 660ttcctctccg
actggatcgt gtacaaccgc ttccgccgcc agctcaccat ctccgtcctg
720gacatcgtcg ccttctttcc caactacgac atccgcacct accctatcca
gaccgccacc 780cagctcaccc gcgaggtcta cctcgacctc ccgttcatca
acgagaacct cagcccggcc 840gccagctacc cgaccttctc cgccgctgag
tccgccatca ttcgcagccc gcacctcgtg 900gacttcctca actccttcac
catctacacc gactccctcg cccgctacgc ctactggggc 960ggtcacctcg
tgaactcctt ccgcaccggc accactacca acctcatccg cagcccgctc
1020tacggccgcg agggcaacac cgagcgcccg gtgaccatca ccgccagccc
gagcgtgccc 1080atcttccgca ccctcagcta catcaccggc ctggacaaca
gcaaccctgt ggcgggcatc 1140gagggcgtgg agttccagaa caccatctcc
aggagcatct accgcaagag cggccctatc 1200gacagcttca gcgagctgcc
tcctcaggac gccagcgtga gccctgccat cggctacagc 1260cacaggctgt
gccacgccac cttcctggag cgcatcagcg gccctcgcat cgcgggcacc
1320gtgttctcgt ggacccaccg cagcgcctct cctacgaacg aggtgtctcc
tagtcgcatc 1380acccagatcc cttgggtcaa ggcccacacc ctggctagtg
gcgctagtgt catcaagggc 1440cctggcttca ccggtggtga catcctgacc
aggaactcta tgggcgagct gggcactctg 1500agggtcactt tcactggccg
cctgcctcag tcttactaca tccgcttccg ctacgctagt 1560gtcgctaacc
gctctggtac tttccgctac tctcagcctc cgtcttacgg tatctctttc
1620cctaagacta tggacgctgg tgagcctctg accagtagga gcttcgctca
cactactctg 1680ttcactccta tcactttctc tagggctcag gaggagttcg
acctgtacat ccagtctggt 1740gtgtacatcg acaggatcga gttcatcccc
gtgaccgcca cgttcgaggc cgagtacgac 1800cttgagcgcg cccagaaggc
tgtcaatgag ctcttcacgt ccagcaatca gatcggcctg 1860aagaccgacg
tcactgacta ccacatcgac caagtctcca acctcgtgga gtgcctctcc
1920gatgagttct gcctcgacga gaagaaggag ctgtccgaga aggtgaagca
tgccaagcgt 1980ctcagcgacg agaggaatct cctccaggac cccaatttcc
gcggcatcaa caggcagctc 2040gaccgcggct ggcgcggcag caccgacatc
acgatccagg gcggcgacga tgtgttcaag 2100gagaactacg tgactctcct
gggcactttc gacgagtgct accctaccta cttgtaccag 2160aagatcgatg
agtccaagct caaggcttac actcgctacc agctccgcgg ctacatcgaa
2220gacagccaag acctcgagat ttacctgatc cgctacaacg ccaagcacga
gaccgtcaac 2280gtgcccggta ctggttccct ctggccgctg agcgccccca
gcccgatcgg caagtgtgcc 2340caccacagcc accacttctc cttggacatc
gatgtgggct gcaccgacct gaacgaggac 2400ctcggagtct gggtcatctt
caagatcaag acccaggacg gccacgcgcg cctgggcaac 2460ctggagttcc
tcgaggagaa gcccctggtc ggtgaggctc tggccagggt caagagggct
2520gagaagaagt ggagggacaa gcgcgagaag ctcgagtggg agaccaacat
cgtttacaag 2580gaggccaagg agagcgtcga cgccctgttc gtgaactccc
agtacgaccg cctgcaggcc 2640gacaccaaca tcgccatgat ccacgctgcc
gacaagaggg tgcacagcat tcgcgaggcc 2700tacctgcctg agctgtccgt
gatccctggt gtgaacgctg ccatctttga ggagctggag 2760ggccgcatct
ttaccgcatt ctccctgtac gacgcccgca acgtgatcaa gaacggtgac
2820ttcaacaatg gcctcagctg ctggaacgtc aagggccacg tggacgtcga
ggaacagaac 2880aaccaccgct ccgtcctggt cgtcccagag tgggaggctg
aggtctccca agaggtccgc 2940gtctgcccag gccgcggcta cattctcagg
gtcaccgctt acaaggaggg ctacggtgag 3000ggctgtgtga ccatccacga
gatcgagaac aacaccgacg agcttaagtt ctccaactgc 3060gtggaggagg
aggtgtaccc aaacaacacc gttacttgca acgactacac cgccacccag
3120gaggagtacg agggcaccta cacttccagg aacaggggct acgatggtgc
ctacgagagc 3180aacagcagcg ttcctgctga ctacgcttcc gcctacgagg
agaaggccta cacggatggc 3240cgcagggaca acccttgcga gagcaaccgc
ggctacggcg actacactcc cctgcccgcc 3300ggctacgtta ccaaggagct
ggagtacttc ccggagactg acaaggtgtg gatcgagatc 3360ggcgagaccg
agggcacctt catcgtggac agcgtggagc tgctcctgat ggaggagtag
3420141139PRTArtificial sequenceAmino acid sequence of the chimeric
protein TIC844. 14Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr
Asn Cys Leu Ser1 5 10 15Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg
Leu Glu Thr Gly Asn 20 25 30Thr Val Ala Asp Ile Ser Leu Gly Leu Ile
Asn Phe Leu Tyr Ser Asn 35 40 45Phe Val Pro Gly Gly Gly Phe Ile Val
Gly Leu Leu Glu Leu Ile Trp 50 55 60Gly Phe Ile Gly Pro Ser Gln Trp
Asp Ile Phe Leu Ala Gln Ile Glu65 70 75 80Gln Leu Ile Ser Gln Arg
Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 85 90 95Ser Arg Leu Glu Gly
Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 100 105 110Phe Ser Asp
Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 115 120 125Met
Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 130 135
140Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val
Tyr145 150 155 160Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg
Asp Val Ser Val 165 170 175Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala
Thr Ile Asn Asn Arg Tyr 180 185 190Ser Asp Leu Thr Ser Leu Ile His
Val Tyr Thr Asn His Cys Val Asp 195 200 205Thr Tyr Asn Gln Gly Leu
Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 210 215 220Trp Ile Val Tyr
Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu225 230 235 240Asp
Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 245 250
255Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe
260 265 270Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe
Ser Ala 275 280 285Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val
Asp Phe Leu Asn 290 295 300Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala
Arg Tyr Ala Tyr Trp Gly305 310 315 320Gly His Leu Val Asn Ser Phe
Arg Thr Gly Thr Thr Thr Asn Leu Ile 325 330 335Arg Ser Pro Leu Tyr
Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 340 345 350Ile Thr Ala
Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Ile 355 360 365Thr
Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 370 375
380Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro
Ile385 390 395 400Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser
Val Ser Pro Ala 405 410 415Ile Gly Tyr Ser His Arg Leu Cys His Ala
Thr Phe Leu Glu Arg Ile 420 425 430Ser Gly Pro Arg Ile Ala Gly Thr
Val Phe Ser Trp Thr His Arg Ser 435 440 445Ala Ser Pro Thr Asn Glu
Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 450 455 460Trp Val Lys Ala
His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly465 470 475 480Pro
Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 485 490
495Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr
500 505 510Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly
Thr Phe 515 520 525Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe
Pro Lys Thr Met 530 535 540Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser
Phe Ala His Thr Thr Leu545 550 555 560Phe Thr Pro Ile Thr Phe Ser
Arg Ala Gln Glu Glu Phe Asp Leu Tyr 565 570 575Ile Gln Ser Gly Val
Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 580 585 590Ala Thr Phe
Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val 595 600 605Asn
Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val 610 615
620Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu
Ser625 630 635 640Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser
Glu Lys Val Lys 645 650 655His Ala Lys Arg Leu Ser Asp Glu Arg Asn
Leu Leu Gln Asp Pro Asn 660 665 670Phe Arg Gly Ile Asn Arg Gln Leu
Asp Arg Gly Trp Arg Gly Ser Thr 675 680 685Asp Ile Thr Ile Gln Gly
Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 690 695 700Thr Leu Leu Gly
Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln705 710 715 720Lys
Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 725 730
735Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr
740 745 750Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser
Leu Trp 755 760 765Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Ala
His His Ser His 770 775 780His Phe Ser Leu Asp Ile Asp Val Gly Cys
Thr Asp Leu Asn Glu Asp785 790 795 800Leu Gly Val Trp Val Ile Phe
Lys Ile Lys Thr Gln Asp Gly His Ala 805 810 815Arg Leu Gly Asn Leu
Glu Phe Leu Glu Glu Lys Pro Leu Val Gly Glu 820 825 830Ala Leu Ala
Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg 835 840 845Glu
Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu 850 855
860Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln
Ala865 870 875 880Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys
Arg Val His Ser 885 890 895Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser
Val Ile Pro Gly Val Asn 900 905 910Ala Ala Ile Phe Glu Glu Leu Glu
Gly Arg Ile Phe Thr Ala Phe Ser 915 920 925Leu Tyr Asp Ala Arg Asn
Val Ile Lys Asn Gly Asp Phe Asn Asn Gly 930 935 940Leu Ser Cys Trp
Asn Val Lys Gly His Val Asp Val Glu Glu Gln Asn945 950 955 960Asn
His Arg Ser Val Leu Val Val Pro Glu Trp Glu Ala Glu Val Ser 965 970
975Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr
980 985 990Ala Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile His
Glu Ile 995 1000 1005Glu Asn Asn Thr Asp Glu Leu Lys Phe Ser Asn
Cys Val Glu Glu 1010 1015 1020Glu Val Tyr Pro Asn Asn Thr Val Thr
Cys Asn Asp Tyr Thr Ala 1025 1030 1035Thr Gln Glu Glu Tyr Glu Gly
Thr Tyr Thr Ser Arg Asn Arg Gly 1040 1045 1050Tyr Asp Gly Ala Tyr
Glu Ser Asn Ser Ser Val Pro Ala Asp Tyr 1055 1060 1065Ala Ser Ala
Tyr Glu Glu Lys Ala Tyr Thr Asp Gly Arg Arg Asp 1070 1075 1080Asn
Pro Cys Glu Ser Asn Arg Gly Tyr Gly Asp
Tyr Thr Pro Leu 1085 1090 1095Pro Ala Gly Tyr Val Thr Lys Glu Leu
Glu Tyr Phe Pro Glu Thr 1100 1105 1110Asp Lys Val Trp Ile Glu Ile
Gly Glu Thr Glu Gly Thr Phe Ile 1115 1120 1125Val Asp Ser Val Glu
Leu Leu Leu Met Glu Glu 1130 1135153420DNAArtificial
sequenceNucleotide sequence used for expression in a bacterial cell
encoding TIC844_2. 15atggaaataa ataatcaaaa ccaatgtgtg ccttacaatt
gtttaagtaa tcctaaggag 60ataatattag gcgaggaaag gctagaaaca gggaatactg
tagcagacat ttcattaggg 120cttattaatt ttctatattc taattttgta
ccaggaggag gatttatagt aggtttacta 180gaattaatat ggggatttat
agggccttcg caatgggata tttttttagc tcaaattgag 240caattgatta
gtcaaagaat agaagaattt gctaggaatc aggcaatttc aagattggag
300gggctaagca atctttataa ggtctatgtt agagcgttta gcgactggga
gaaagatcct 360actaatcctg ctttaaggga agaaatgcgt atacaattta
atgacatgaa tagtgctctc 420ataacggcta ttccactttt tagagttcaa
aattatgaag ttgctctttt atctgtatat 480gttcaagccg caaacttaca
tttatctatt ttaagggatg tttcagtttt cggagaaaga 540tggggatatg
atacagcgac tatcaataat cgctatagtg atctgactag ccttattcat
600gtttatacta accattgtgt ggatacgtat aatcagggat taaggcgttt
ggaaggtcgt 660tttcttagcg attggattgt atataatcgt ttccggagac
aattgacaat ttcagtatta 720gatattgttg cgttttttcc aaattatgat
attagaacat atccaattca aacagctact 780cagctaacga gggaagtcta
tctggattta ccttttatta atgaaaatct ttctcctgca 840gcaagctatc
caaccttttc agctgctgaa agtgctataa ttagaagtcc tcatttagta
900gactttttaa atagctttac catttataca gatagtctgg cacgttctgc
atattgggga 960gggcacttgg taaattcttt ccgcacagga accactacta
atttgataag atccccttta 1020tatggaaggg aaggaaatac agagcgcccc
gtaactatta ccgcatcacc tagcgtacca 1080atatttagaa cactttcata
tattacaggc cttgacaatt cacatcctgt agctggaatc 1140gagggagtgg
aattccaaaa tactataagt agaagtatct atcgtaaaag cggtccaata
1200gattctttta gtgaattacc acctcaagat gccagcgtat ctcctgcaat
tgggtatagt 1260caccgtttat gccatgcaac atttttagaa cggttaagtg
gaccaagaat agcaggcacc 1320gtattttctt ggacacaccg tagtgccagc
cctactaacg aagtaagtcc atctagaatt 1380acacaaattc catgggtaaa
ggcgcatact cttgcatctg gtgcctccgt cattaaaggt 1440cctggattta
caggtggaga tattctgact aggaatagta tgggcgagct ggggacctta
1500cgagtaacct tcacaggaag attaccacaa agttattata tacgtttccg
ttatgcttcg 1560gtagcaaata ggagtggtac atttagatat tcacagccac
cttcgtatgg aatttcattt 1620ccaaaaacta tggacgcagg tgaaccacta
acatctcgtt cgttcgctca tacaacactc 1680ttcactccaa taaccttttc
acgagctcaa gaagaatttg atctatacat ccaatcgggt 1740gtttatatag
atcgaattga atttataccg gttactgcaa catttgaggc agaatatgat
1800ttagaaagag cacaaaaggc ggtgaatgag ctgtttactt cttccaatca
aatcgggtta 1860aaaacagatg tgacggatta tcatattgat caagtatcca
atttagttga gtgtttatct 1920gatgaatttt gtctggatga aaaaaaagaa
ttgtccgaga aagtcaaaca tgcgaagcga 1980cttagtgatg agcggaattt
acttcaagat ccaaacttta gagggatcaa tagacaacta 2040gaccgtggct
ggagaggaag tacggatatt accatccaag gaggcgatga cgtattcaaa
2100gagaattacg ttacgctatt gggtaccttt gatgagtgct atccaacgta
tttatatcaa 2160aaaatagatg agtcgaaatt aaaagcctat acccgttacc
aattaagagg gtatatcgaa 2220gatagtcaag acttagaaat ctatttaatt
cgctacaatg ccaaacacga aacagtaaat 2280gtgccaggta cgggttcctt
atggccgctt tcagccccaa gtccaatcgg aaaatgtgcc 2340catcattccc
atcatttctc cttggacatt gatgttggat gtacagactt aaatgaggac
2400ttaggtgtat gggtgatatt caagattaag acgcaagatg gccatgcaag
actaggaaat 2460ctagaatttc tcgaagagaa accattagta ggagaagcac
tagctcgtgt gaaaagagcg 2520gagaaaaaat ggagagacaa acgtgaaaaa
ttggaatggg aaacaaatat tgtttataaa 2580gaggcaaaag aatctgtaga
tgctttattt gtaaactctc aatatgatag attacaagcg 2640gataccaaca
tcgcgatgat tcatgcggca gataaacgcg ttcatagcat tcgagaagct
2700tatctgcctg agctgtctgt gattccgggt gtcaatgcgg ctatttttga
agaattagaa 2760gggcgtattt tcactgcatt ctccctatat gatgcgagaa
atgtcattaa aaatggtgat 2820tttaataatg gcttatcctg ctggaacgtg
aaagggcatg tagatgtaga agaacaaaac 2880aaccaccgtt cggtccttgt
tgttccggaa tgggaagcag aagtgtcaca agaagttcgt 2940gtctgtccgg
gtcgtggcta tatccttcgt gtcacagcgt acaaggaggg atatggagaa
3000ggttgcgtaa ccattcatga gatcgagaac aatacagacg aactgaagtt
tagcaactgt 3060gtagaagagg aagtatatcc aaacaacacg gtaacgtgta
atgattatac tgcgactcaa 3120gaagaatatg agggtacgta cacttctcgt
aatcgaggat atgacggagc ctatgaaagc 3180aattcttctg taccagctga
ttatgcatca gcctatgaag aaaaagcata tacagatgga 3240cgaagagaca
atccttgtga atctaacaga ggatatgggg attacacacc actaccagct
3300ggctatgtga caaaagaatt agagtacttc ccagaaaccg ataaggtatg
gattgagatc 3360ggagaaacgg aaggaacatt catcgtggac agcgtggaat
tacttcttat ggaggaatag 3420161139PRTArtificial sequenceAmino acid
sequence of the engineered insecticidal chimeric protein TIC844_2.
16Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser1
5 10 15Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly
Asn 20 25 30Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr
Ser Asn 35 40 45Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu
Leu Ile Trp 50 55 60Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu
Ala Gln Ile Glu65 70 75 80Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe
Ala Arg Asn Gln Ala Ile 85 90 95Ser Arg Leu Glu Gly Leu Ser Asn Leu
Tyr Lys Val Tyr Val Arg Ala 100 105 110Phe Ser Asp Trp Glu Lys Asp
Pro Thr Asn Pro Ala Leu Arg Glu Glu 115 120 125Met Arg Ile Gln Phe
Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 130 135 140Pro Leu Phe
Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr145 150 155
160Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val
165 170 175Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn
Arg Tyr 180 185 190Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn
His Cys Val Asp 195 200 205Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu
Gly Arg Phe Leu Ser Asp 210 215 220Trp Ile Val Tyr Asn Arg Phe Arg
Arg Gln Leu Thr Ile Ser Val Leu225 230 235 240Asp Ile Val Ala Phe
Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 245 250 255Gln Thr Ala
Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 260 265 270Ile
Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser Ala 275 280
285Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn
290 295 300Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr
Trp Gly305 310 315 320Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr
Thr Thr Asn Leu Ile 325 330 335Arg Ser Pro Leu Tyr Gly Arg Glu Gly
Asn Thr Glu Arg Pro Val Thr 340 345 350Ile Thr Ala Ser Pro Ser Val
Pro Ile Phe Arg Thr Leu Ser Tyr Ile 355 360 365Thr Gly Leu Asp Asn
Ser His Pro Val Ala Gly Ile Glu Gly Val Glu 370 375 380Phe Gln Asn
Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile385 390 395
400Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala
405 410 415Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu
Arg Leu 420 425 430Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp
Thr His Arg Ser 435 440 445Ala Ser Pro Thr Asn Glu Val Ser Pro Ser
Arg Ile Thr Gln Ile Pro 450 455 460Trp Val Lys Ala His Thr Leu Ala
Ser Gly Ala Ser Val Ile Lys Gly465 470 475 480Pro Gly Phe Thr Gly
Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 485 490 495Leu Gly Thr
Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 500 505 510Tyr
Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 515 520
525Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met
530 535 540Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr
Thr Leu545 550 555 560Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu
Glu Phe Asp Leu Tyr 565 570 575Ile Gln Ser Gly Val Tyr Ile Asp Arg
Ile Glu Phe Ile Pro Val Thr 580 585 590Ala Thr Phe Glu Ala Glu Tyr
Asp Leu Glu Arg Ala Gln Lys Ala Val 595 600 605Asn Glu Leu Phe Thr
Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val 610 615 620Thr Asp Tyr
His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser625 630 635
640Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys
645 650 655His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp
Pro Asn 660 665 670Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp
Arg Gly Ser Thr 675 680 685Asp Ile Thr Ile Gln Gly Gly Asp Asp Val
Phe Lys Glu Asn Tyr Val 690 695 700Thr Leu Leu Gly Thr Phe Asp Glu
Cys Tyr Pro Thr Tyr Leu Tyr Gln705 710 715 720Lys Ile Asp Glu Ser
Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 725 730 735Gly Tyr Ile
Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 740 745 750Asn
Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp 755 760
765Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Ala His His Ser His
770 775 780His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn
Glu Asp785 790 795 800Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr
Gln Asp Gly His Ala 805 810 815Arg Leu Gly Asn Leu Glu Phe Leu Glu
Glu Lys Pro Leu Val Gly Glu 820 825 830Ala Leu Ala Arg Val Lys Arg
Ala Glu Lys Lys Trp Arg Asp Lys Arg 835 840 845Glu Lys Leu Glu Trp
Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu 850 855 860Ser Val Asp
Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala865 870 875
880Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg Val His Ser
885 890 895Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly
Val Asn 900 905 910Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile Phe
Thr Ala Phe Ser 915 920 925Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn
Gly Asp Phe Asn Asn Gly 930 935 940Leu Ser Cys Trp Asn Val Lys Gly
His Val Asp Val Glu Glu Gln Asn945 950 955 960Asn His Arg Ser Val
Leu Val Val Pro Glu Trp Glu Ala Glu Val Ser 965 970 975Gln Glu Val
Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr 980 985 990Ala
Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile His Glu Ile 995
1000 1005Glu Asn Asn Thr Asp Glu Leu Lys Phe Ser Asn Cys Val Glu
Glu 1010 1015 1020Glu Val Tyr Pro Asn Asn Thr Val Thr Cys Asn Asp
Tyr Thr Ala 1025 1030 1035Thr Gln Glu Glu Tyr Glu Gly Thr Tyr Thr
Ser Arg Asn Arg Gly 1040 1045 1050Tyr Asp Gly Ala Tyr Glu Ser Asn
Ser Ser Val Pro Ala Asp Tyr 1055 1060 1065Ala Ser Ala Tyr Glu Glu
Lys Ala Tyr Thr Asp Gly Arg Arg Asp 1070 1075 1080Asn Pro Cys Glu
Ser Asn Arg Gly Tyr Gly Asp Tyr Thr Pro Leu 1085 1090 1095Pro Ala
Gly Tyr Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu Thr 1100 1105
1110Asp Lys Val Trp Ile Glu Ile Gly Glu Thr Glu Gly Thr Phe Ile
1115 1120 1125Val Asp Ser Val Glu Leu Leu Leu Met Glu Glu 1130
1135173420DNAArtificial sequenceNucleotide sequence used for
expression in a bacterial cell encoding TIC844_4. 17atggaaataa
ataatcaaaa ccaatgtgtg ccttacaatt gtttaagtaa tcctaaggag 60ataatattag
gcgaggaaag gctagaaaca gggaatactg tagcagacat ttcattaggg
120cttattaatt ttctatattc taattttgta ccaggaggag gatttatagt
aggtttacta 180gaattaatat ggggatttat agggccttcg caatgggata
tttttttagc tcaaattgag 240caattgatta gtcaaagaat agaagaattt
gctaggaatc aggcaatttc aagattggag 300gggctaagca atctttataa
ggtctatgtt agagcgttta gcgactggga gaaagatcct 360actaatcctg
ctttaaggga agaaatgcgt atacaattta atgacatgaa tagtgctctc
420ataacggcta ttccactttt tagagttcaa aattatgaag ttgctctttt
atctgtatat 480gttcaagccg caaacttaca tttatctatt ttaagggatg
tttcagtttt cggagaaaga 540tggggatatg atacagcgac tatcaataat
cgctatagtg atctgactag ccttattcat 600gtttatacta accattgtgt
ggatacgtat aatcagggat taaggcgttt ggaaggtcgt 660tttcttagcg
attggattgt atataatcgt ttccggagac aattgacaat ttcagtatta
720gatattgttg cgttttttcc aaattatgat attagaacat atccaattca
aacagctact 780cagctaacga gggaagtcta tctggattta ccttttatta
atgaaaatct ttctcctgca 840gcaagctatc caaccttttc agctgctgaa
agtgctataa ttagaagtcc tcatttagta 900gactttttaa atagctttac
catttataca gatagtctgg cacgtagtgc atattgggga 960gggcacttgg
taaattcttt ccgcacagga accactacta atttgataag atccccttta
1020tatggaaggg aaggaaatac agagcgcccc gtaactatta ccgcatcacc
tagcgtacca 1080atatttagaa cactttcata tattacaggc cttgacaatt
caaatcctgt agctggaatc 1140gagggagtgg aattccaaaa tactataagt
agaagtatct atcgtaaaag cggtccaata 1200gattctttta gtgaattacc
acctcaagat gccagcgtat ctcctgcaat tgggtatagt 1260caccgtttat
gccatgcaac atttttagaa cggattagtg gaccaagaat agcaggcacc
1320gtattttctt ggacacaccg tagtgccagc cctactaacg aagtaagtcc
atctagaatt 1380acacaaattc catgggtaaa ggcgcatact cttgcatctg
gtgcctccgt cattaaaggt 1440cctggattta caggtggaga tattctgact
aggaatagta tgggcgagct ggggacctta 1500cgagtaacct tcacaggaag
attaccacaa agttattata tacgtttccg ttatgcttcg 1560gtagcaaata
ggagtggtac atttagatat tcacagccac cttcgtatgg aatttcattt
1620ccaaaaacta tggacgcagg tgaaccacta acatctcgtt cgttcgctca
tacaacactc 1680ttcactccaa taaccttttc acgagctcaa gaagaatttg
atctatacat ccaatcgggt 1740gtttatatag atcgaattga atttataccg
gttactgcaa catttgaggc agaatatgat 1800ttagaaagag cacaaaaggc
ggtgaatgag ctgtttactt cttccaatca aatcgggtta 1860aaaacagatg
tgacggatta tcatattgat caagtatcca atttagttga gtgtttatct
1920gatgaatttt gtctggatga aaaaaaagaa ttgtccgaga aagtcaaaca
tgcgaagcga 1980cttagtgatg agcggaattt acttcaagat ccaaacttta
gagggatcaa tagacaacta 2040gaccgtggct ggagaggaag tacggatatt
accatccaag gaggcgatga cgtattcaaa 2100gagaattacg ttacgctatt
gggtaccttt gatgagtgct atccaacgta tttatatcaa 2160aaaatagatg
agtcgaaatt aaaagcctat acccgttacc aattaagagg gtatatcgaa
2220gatagtcaag acttagaaat ctatttaatt cgctacaatg ccaaacacga
aacagtaaat 2280gtgccaggta cgggttcctt atggccgctt tcagccccaa
gtccaatcgg aaaatgtgcc 2340catcattccc atcatttctc cttggacatt
gatgttggat gtacagactt aaatgaggac 2400ttaggtgtat gggtgatatt
caagattaag acgcaagatg gccatgcaag actaggaaat 2460ctagaatttc
tcgaagagaa accattagta ggagaagcac tagctcgtgt gaaaagagcg
2520gagaaaaaat ggagagacaa acgtgaaaaa ttggaatggg aaacaaatat
tgtttataaa 2580gaggcaaaag aatctgtaga tgctttattt gtaaactctc
aatatgatag attacaagcg 2640gataccaaca tcgcgatgat tcatgcggca
gataaacgcg ttcatagcat tcgagaagct 2700tatctgcctg agctgtctgt
gattccgggt gtcaatgcgg ctatttttga agaattagaa 2760gggcgtattt
tcactgcatt ctccctatat gatgcgagaa atgtcattaa aaatggtgat
2820tttaataatg gcttatcctg ctggaacgtg aaagggcatg tagatgtaga
agaacaaaac 2880aaccaccgtt cggtccttgt tgttccggaa tgggaagcag
aagtgtcaca agaagttcgt 2940gtctgtccgg gtcgtggcta tatccttcgt
gtcacagcgt acaaggaggg atatggagaa 3000ggttgcgtaa ccattcatga
gatcgagaac aatacagacg aactgaagtt tagcaactgt 3060gtagaagagg
aagtatatcc aaacaacacg gtaacgtgta atgattatac tgcgactcaa
3120gaagaatatg agggtacgta cacttctcgt aatcgaggat atgacggagc
ctatgaaagc 3180aattcttctg taccagctga ttatgcatca gcctatgaag
aaaaagcata tacagatgga 3240cgaagagaca atccttgtga atctaacaga
ggatatgggg attacacacc actaccagct 3300ggctatgtga caaaagaatt
agagtacttc ccagaaaccg ataaggtatg gattgagatc 3360ggagaaacgg
aaggaacatt catcgtggac agcgtggaat tacttcttat ggaggaatag
3420181139PRTArtificial sequenceAmino acid sequence of the
engineered insecticidal chimeric protein TIC844_4. 18Met Glu Ile
Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser1 5 10 15Asn Pro
Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 20 25 30Thr
Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 35 40
45Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp
50 55 60Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile
Glu65 70 75 80Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn
Gln Ala Ile 85 90 95Ser
Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 100 105
110Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu
115 120 125Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr
Ala Ile 130 135 140Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu
Leu Ser Val Tyr145 150 155 160Val Gln Ala Ala Asn Leu His Leu Ser
Ile Leu Arg Asp Val Ser Val 165 170 175Phe Gly Glu Arg Trp Gly Tyr
Asp Thr Ala Thr Ile Asn Asn Arg Tyr 180 185 190Ser Asp Leu Thr Ser
Leu Ile His Val Tyr Thr Asn His Cys Val Asp 195 200 205Thr Tyr Asn
Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 210 215 220Trp
Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu225 230
235 240Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro
Ile 245 250 255Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp
Leu Pro Phe 260 265 270Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr
Pro Thr Phe Ser Ala 275 280 285Ala Glu Ser Ala Ile Ile Arg Ser Pro
His Leu Val Asp Phe Leu Asn 290 295 300Ser Phe Thr Ile Tyr Thr Asp
Ser Leu Ala Arg Ser Ala Tyr Trp Gly305 310 315 320Gly His Leu Val
Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 325 330 335Arg Ser
Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 340 345
350Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Ile
355 360 365Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly
Val Glu 370 375 380Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys
Ser Gly Pro Ile385 390 395 400Asp Ser Phe Ser Glu Leu Pro Pro Gln
Asp Ala Ser Val Ser Pro Ala 405 410 415Ile Gly Tyr Ser His Arg Leu
Cys His Ala Thr Phe Leu Glu Arg Ile 420 425 430Ser Gly Pro Arg Ile
Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 435 440 445Ala Ser Pro
Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 450 455 460Trp
Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly465 470
475 480Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly
Glu 485 490 495Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro
Gln Ser Tyr 500 505 510Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn
Arg Ser Gly Thr Phe 515 520 525Arg Tyr Ser Gln Pro Pro Ser Tyr Gly
Ile Ser Phe Pro Lys Thr Met 530 535 540Asp Ala Gly Glu Pro Leu Thr
Ser Arg Ser Phe Ala His Thr Thr Leu545 550 555 560Phe Thr Pro Ile
Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 565 570 575Ile Gln
Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 580 585
590Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val
595 600 605Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr
Asp Val 610 615 620Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val
Glu Cys Leu Ser625 630 635 640Asp Glu Phe Cys Leu Asp Glu Lys Lys
Glu Leu Ser Glu Lys Val Lys 645 650 655His Ala Lys Arg Leu Ser Asp
Glu Arg Asn Leu Leu Gln Asp Pro Asn 660 665 670Phe Arg Gly Ile Asn
Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr 675 680 685Asp Ile Thr
Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 690 695 700Thr
Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln705 710
715 720Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu
Arg 725 730 735Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu
Ile Arg Tyr 740 745 750Asn Ala Lys His Glu Thr Val Asn Val Pro Gly
Thr Gly Ser Leu Trp 755 760 765Pro Leu Ser Ala Pro Ser Pro Ile Gly
Lys Cys Ala His His Ser His 770 775 780His Phe Ser Leu Asp Ile Asp
Val Gly Cys Thr Asp Leu Asn Glu Asp785 790 795 800Leu Gly Val Trp
Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala 805 810 815Arg Leu
Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu Val Gly Glu 820 825
830Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg
835 840 845Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala
Lys Glu 850 855 860Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp
Arg Leu Gln Ala865 870 875 880Asp Thr Asn Ile Ala Met Ile His Ala
Ala Asp Lys Arg Val His Ser 885 890 895Ile Arg Glu Ala Tyr Leu Pro
Glu Leu Ser Val Ile Pro Gly Val Asn 900 905 910Ala Ala Ile Phe Glu
Glu Leu Glu Gly Arg Ile Phe Thr Ala Phe Ser 915 920 925Leu Tyr Asp
Ala Arg Asn Val Ile Lys Asn Gly Asp Phe Asn Asn Gly 930 935 940Leu
Ser Cys Trp Asn Val Lys Gly His Val Asp Val Glu Glu Gln Asn945 950
955 960Asn His Arg Ser Val Leu Val Val Pro Glu Trp Glu Ala Glu Val
Ser 965 970 975Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu
Arg Val Thr 980 985 990Ala Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val
Thr Ile His Glu Ile 995 1000 1005Glu Asn Asn Thr Asp Glu Leu Lys
Phe Ser Asn Cys Val Glu Glu 1010 1015 1020Glu Val Tyr Pro Asn Asn
Thr Val Thr Cys Asn Asp Tyr Thr Ala 1025 1030 1035Thr Gln Glu Glu
Tyr Glu Gly Thr Tyr Thr Ser Arg Asn Arg Gly 1040 1045 1050Tyr Asp
Gly Ala Tyr Glu Ser Asn Ser Ser Val Pro Ala Asp Tyr 1055 1060
1065Ala Ser Ala Tyr Glu Glu Lys Ala Tyr Thr Asp Gly Arg Arg Asp
1070 1075 1080Asn Pro Cys Glu Ser Asn Arg Gly Tyr Gly Asp Tyr Thr
Pro Leu 1085 1090 1095Pro Ala Gly Tyr Val Thr Lys Glu Leu Glu Tyr
Phe Pro Glu Thr 1100 1105 1110Asp Lys Val Trp Ile Glu Ile Gly Glu
Thr Glu Gly Thr Phe Ile 1115 1120 1125Val Asp Ser Val Glu Leu Leu
Leu Met Glu Glu 1130 1135193420DNAArtificial sequenceNucleotide
sequence used for expression in a bacterial cell encoding TIC844_5.
19atggaaataa ataatcaaaa ccaatgtgtg ccttacaatt gtttaagtaa tcctaaggag
60ataatattag gcgaggaaag gctagaaaca gggaatactg tagcagacat ttcattaggg
120cttattaatt ttctatattc taattttgta ccaggaggag gatttatagt
aggtttacta 180gaattaatat ggggatttat agggccttcg caatgggata
tttttttagc tcaaattgag 240caattgatta gtcaaagaat agaagaattt
gctaggaatc aggcaatttc aagattggag 300gggctaagca atctttataa
ggtctatgtt agagcgttta gcgactggga gaaagatcct 360actaatcctg
ctttaaggga agaaatgcgt atacaattta atgacatgaa tagtgctctc
420ataacggcta ttccactttt tagagttcaa aattatgaag ttgctctttt
atctgtatat 480gttcaagccg caaacttaca tttatctatt ttaagggatg
tttcagtttt cggagaaaga 540tggggatatg atacagcgac tatcaataat
cgctatagtg atctgactag ccttattcat 600gtttatacta accattgtgt
ggatacgtat aatcagggat taaggcgttt ggaaggtcgt 660tttcttagcg
attggattgt atataatcgt ttccggagac aattgacaat ttcagtatta
720gatattgttg cgttttttcc aaattatgat attagaacat atccaattca
aacagctact 780cagctaacga gggaagtcta tctggattta ccttttatta
atgaaaatct ttctcctgca 840gcaaaatatc caaccttttc agctgctgaa
agtgctataa ttagaagtcc tcatttagta 900gactttttaa atagctttac
catttataca gatagtctgg cacgttctgc atattgggga 960gggcacttgg
taaattcttt ccgcacagga accactacta atttgataag atccccttta
1020tatggaaggg aaggaaatac agagcgcccc gtaactatta ccgcatcacc
tagcgtacca 1080atatttagaa cactttcata tccaacaggc cttgacaatt
caaatcctgt agctggaatc 1140gagggagtgg aattccaaaa tactataagt
agaagtatct atcgtaaaag cggtccaata 1200gattctttta gtgaattacc
acctcaagat gccagcgtat ctcctgcaat tgggtatagt 1260caccgtttat
gccatgcaac atttttagaa cggattagtg gaccaagaat agcaggcacc
1320gtattttctt ggacacaccg tagtgccagc cctactaacg aagtaagtcc
atctagaatt 1380acacaaattc catgggtaaa ggcgcatact cttgcatctg
gtgcctccgt cattaaaggt 1440cctggattta caggtggaga tattctgact
aggaatagta tgggcgagct ggggacctta 1500cgagtaacct tcacaggaag
attaccacaa agttattata tacgtttccg ttatgcttcg 1560gtagcaaata
ggagtggtac atttagatat tcacagccac cttcgtatgg aatttcattt
1620ccaaaaacta tggacgcagg tgaaccacta acatctcgtt cgttcgctca
tacaacactc 1680ttcactccaa taaccttttc acgagctcaa gaagaatttg
atctatacat ccaatcgggt 1740gtttatatag atcgaattga atttataccg
gttactgcaa catttgaggc agaatatgat 1800ttagaaagag cacaaaaggc
ggtgaatgag ctgtttactt cttccaatca aatcgggtta 1860aaaacagatg
tgacggatta tcatattgat caagtatcca atttagttga gtgtttatct
1920gatgaatttt gtctggatga aaaaaaagaa ttgtccgaga aagtcaaaca
tgcgaagcga 1980cttagtgatg agcggaattt acttcaagat ccaaacttta
gagggatcaa tagacaacta 2040gaccgtggct ggagaggaag tacggatatt
accatccaag gaggcgatga cgtattcaaa 2100gagaattacg ttacgctatt
gggtaccttt gatgagtgct atccaacgta tttatatcaa 2160aaaatagatg
agtcgaaatt aaaagcctat acccgttacc aattaagagg gtatatcgaa
2220gatagtcaag acttagaaat ctatttaatt cgctacaatg ccaaacacga
aacagtaaat 2280gtgccaggta cgggttcctt atggccgctt tcagccccaa
gtccaatcgg aaaatgtgcc 2340catcattccc atcatttctc cttggacatt
gatgttggat gtacagactt aaatgaggac 2400ttaggtgtat gggtgatatt
caagattaag acgcaagatg gccatgcaag actaggaaat 2460ctagaatttc
tcgaagagaa accattagta ggagaagcac tagctcgtgt gaaaagagcg
2520gagaaaaaat ggagagacaa acgtgaaaaa ttggaatggg aaacaaatat
tgtttataaa 2580gaggcaaaag aatctgtaga tgctttattt gtaaactctc
aatatgatag attacaagcg 2640gataccaaca tcgcgatgat tcatgcggca
gataaacgcg ttcatagcat tcgagaagct 2700tatctgcctg agctgtctgt
gattccgggt gtcaatgcgg ctatttttga agaattagaa 2760gggcgtattt
tcactgcatt ctccctatat gatgcgagaa atgtcattaa aaatggtgat
2820tttaataatg gcttatcctg ctggaacgtg aaagggcatg tagatgtaga
agaacaaaac 2880aaccaccgtt cggtccttgt tgttccggaa tgggaagcag
aagtgtcaca agaagttcgt 2940gtctgtccgg gtcgtggcta tatccttcgt
gtcacagcgt acaaggaggg atatggagaa 3000ggttgcgtaa ccattcatga
gatcgagaac aatacagacg aactgaagtt tagcaactgt 3060gtagaagagg
aagtatatcc aaacaacacg gtaacgtgta atgattatac tgcgactcaa
3120gaagaatatg agggtacgta cacttctcgt aatcgaggat atgacggagc
ctatgaaagc 3180aattcttctg taccagctga ttatgcatca gcctatgaag
aaaaagcata tacagatgga 3240cgaagagaca atccttgtga atctaacaga
ggatatgggg attacacacc actaccagct 3300ggctatgtga caaaagaatt
agagtacttc ccagaaaccg ataaggtatg gattgagatc 3360ggagaaacgg
aaggaacatt catcgtggac agcgtggaat tacttcttat ggaggaatag
3420201139PRTArtificial sequenceAmino acid sequence of the
engineered insecticidal chimeric protein TIC844_5. 20Met Glu Ile
Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser1 5 10 15Asn Pro
Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 20 25 30Thr
Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 35 40
45Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp
50 55 60Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile
Glu65 70 75 80Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn
Gln Ala Ile 85 90 95Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val
Tyr Val Arg Ala 100 105 110Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn
Pro Ala Leu Arg Glu Glu 115 120 125Met Arg Ile Gln Phe Asn Asp Met
Asn Ser Ala Leu Ile Thr Ala Ile 130 135 140Pro Leu Phe Arg Val Gln
Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr145 150 155 160Val Gln Ala
Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 165 170 175Phe
Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 180 185
190Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp
195 200 205Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu
Ser Asp 210 215 220Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr
Ile Ser Val Leu225 230 235 240Asp Ile Val Ala Phe Phe Pro Asn Tyr
Asp Ile Arg Thr Tyr Pro Ile 245 250 255Gln Thr Ala Thr Gln Leu Thr
Arg Glu Val Tyr Leu Asp Leu Pro Phe 260 265 270Ile Asn Glu Asn Leu
Ser Pro Ala Ala Lys Tyr Pro Thr Phe Ser Ala 275 280 285Ala Glu Ser
Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 290 295 300Ser
Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp Gly305 310
315 320Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu
Ile 325 330 335Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg
Pro Val Thr 340 345 350Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg
Thr Leu Ser Tyr Pro 355 360 365Thr Gly Leu Asp Asn Ser Asn Pro Val
Ala Gly Ile Glu Gly Val Glu 370 375 380Phe Gln Asn Thr Ile Ser Arg
Ser Ile Tyr Arg Lys Ser Gly Pro Ile385 390 395 400Asp Ser Phe Ser
Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 405 410 415Ile Gly
Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 420 425
430Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser
435 440 445Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln
Ile Pro 450 455 460Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser
Val Ile Lys Gly465 470 475 480Pro Gly Phe Thr Gly Gly Asp Ile Leu
Thr Arg Asn Ser Met Gly Glu 485 490 495Leu Gly Thr Leu Arg Val Thr
Phe Thr Gly Arg Leu Pro Gln Ser Tyr 500 505 510Tyr Ile Arg Phe Arg
Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 515 520 525Arg Tyr Ser
Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 530 535 540Asp
Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu545 550
555 560Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu
Tyr 565 570 575Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile
Pro Val Thr 580 585 590Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg
Ala Gln Lys Ala Val 595 600 605Asn Glu Leu Phe Thr Ser Ser Asn Gln
Ile Gly Leu Lys Thr Asp Val 610 615 620Thr Asp Tyr His Ile Asp Gln
Val Ser Asn Leu Val Glu Cys Leu Ser625 630 635 640Asp Glu Phe Cys
Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys 645 650 655His Ala
Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 660 665
670Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr
675 680 685Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn
Tyr Val 690 695 700Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr
Tyr Leu Tyr Gln705 710 715 720Lys Ile Asp Glu Ser Lys Leu Lys Ala
Tyr Thr Arg Tyr Gln Leu Arg 725 730 735Gly Tyr Ile Glu Asp Ser Gln
Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 740 745 750Asn Ala Lys His Glu
Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp 755 760 765Pro Leu Ser
Ala Pro Ser Pro Ile Gly Lys Cys Ala His His Ser His 770 775 780His
Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp785 790
795 800Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His
Ala 805 810 815Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu
Val Gly Glu 820 825 830Ala Leu Ala Arg Val Lys
Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg 835 840 845Glu Lys Leu Glu
Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu 850 855 860Ser Val
Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala865 870 875
880Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg Val His Ser
885 890 895Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly
Val Asn 900 905 910Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile Phe
Thr Ala Phe Ser 915 920 925Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn
Gly Asp Phe Asn Asn Gly 930 935 940Leu Ser Cys Trp Asn Val Lys Gly
His Val Asp Val Glu Glu Gln Asn945 950 955 960Asn His Arg Ser Val
Leu Val Val Pro Glu Trp Glu Ala Glu Val Ser 965 970 975Gln Glu Val
Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr 980 985 990Ala
Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile His Glu Ile 995
1000 1005Glu Asn Asn Thr Asp Glu Leu Lys Phe Ser Asn Cys Val Glu
Glu 1010 1015 1020Glu Val Tyr Pro Asn Asn Thr Val Thr Cys Asn Asp
Tyr Thr Ala 1025 1030 1035Thr Gln Glu Glu Tyr Glu Gly Thr Tyr Thr
Ser Arg Asn Arg Gly 1040 1045 1050Tyr Asp Gly Ala Tyr Glu Ser Asn
Ser Ser Val Pro Ala Asp Tyr 1055 1060 1065Ala Ser Ala Tyr Glu Glu
Lys Ala Tyr Thr Asp Gly Arg Arg Asp 1070 1075 1080Asn Pro Cys Glu
Ser Asn Arg Gly Tyr Gly Asp Tyr Thr Pro Leu 1085 1090 1095Pro Ala
Gly Tyr Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu Thr 1100 1105
1110Asp Lys Val Trp Ile Glu Ile Gly Glu Thr Glu Gly Thr Phe Ile
1115 1120 1125Val Asp Ser Val Glu Leu Leu Leu Met Glu Glu 1130
1135213420DNAArtificial sequenceNucleotide sequence used for
expression in a bacterial cell encoding TIC844_6. 21atggaaataa
ataatcaaaa ccaatgtgtg ccttacaatt gtttaagtaa tcctaaggag 60ataatattag
gcgaggaaag gctagaaaca gggaatactg tagcagacat ttcattaggg
120cttattaatt ttctatattc taattttgta ccaggaggag gatttatagt
aggtttacta 180gaattaatat ggggatttat agggccttcg caatgggata
tttttttagc tcaaattgag 240caattgatta gtcaaagaat agaagaattt
gctaggaatc aggcaatttc aagattggag 300gggctaagca atctttataa
ggtctatgtt agagcgttta gcgactggga gaaagatcct 360actaatcctg
ctttaaggga agaaatgcgt atacaattta atgacatgaa tagtgctctc
420ataacggcta ttccactttt tagagttcaa aattatgaag ttgctctttt
atctgtatat 480gttcaagccg caaacttaca tttatctatt ttaagggatg
tttcagtttt cggagaaaga 540tggggatatg atacagcgac tatcaataat
cgctatagtg atctgactag ccttattcat 600gtttatacta accattgtgt
ggatacgtat aatcagggat taaggcgttt ggaaggtcgt 660tttcttagcg
attggattgt atataatcgt ttccggagac aattgacaat ttcagtatta
720gatattgttg cgttttttcc aaattatgat attagaacat atccaattca
aacagctact 780cagctaacga gggaagtcta tctggattta ccttttatta
atgaaaatct ttctcctgca 840gcaagctatc caaccttttc agctgctgaa
agtgctataa ttagaagtcc tcatttagta 900gactttttaa atagctttac
catttataca gatagtctgg cacgttatgc atattgggga 960gggcacttgg
taaattcttt ccgcacagga accactacta atttgataag atccccttta
1020tatggaaggg aaggaaatac agagcgcccc gtaactatta ccgcatcacc
tagcgtacca 1080atatttagaa cactttcata tattacaggc cttgacaatc
gtaatcctgt agctggaatc 1140gagggagtgg aattccaaaa tactataagt
agaagtatct atcgtaaaag cggtccaata 1200gattctttta gtgaattacc
acctcaagat gccagcgtat ctcctgcaat tgggtatagt 1260caccgtttat
gccatgcaac atttttagaa cggattagtg gaccaagaat agcaggcacc
1320gtattttctt ggacacaccg tagtgccagc cctactaacg aagtaagtcc
atctagaatt 1380acacaaattc catgggtaaa ggcgcatact cttgcatctg
gtgcctccgt cattaaaggt 1440cctggattta caggtggaga tattctgact
aggaatagta tgggcgagct ggggacctta 1500cgagtaacct tcacaggaag
attaccacaa agttattata tacgtttccg ttatgcttcg 1560gtagcaaata
ggagtggtac atttagatat tcacagccac cttcgtatgg aatttcattt
1620ccaaaaacta tggacgcagg tgaaccacta acatctcgtt cgttcgctca
tacaacactc 1680ttcactccaa taaccttttc acgagctcaa gaagaatttg
atctatacat ccaatcgggt 1740gtttatatag atcgaattga atttataccg
gttactgcaa catttgaggc agaatatgat 1800ttagaaagag cacaaaaggc
ggtgaatgag ctgtttactt cttccaatca aatcgggtta 1860aaaacagatg
tgacggatta tcatattgat caagtatcca atttagttga gtgtttatct
1920gatgaatttt gtctggatga aaaaaaagaa ttgtccgaga aagtcaaaca
tgcgaagcga 1980cttagtgatg agcggaattt acttcaagat ccaaacttta
gagggatcaa tagacaacta 2040gaccgtggct ggagaggaag tacggatatt
accatccaag gaggcgatga cgtattcaaa 2100gagaattacg ttacgctatt
gggtaccttt gatgagtgct atccaacgta tttatatcaa 2160aaaatagatg
agtcgaaatt aaaagcctat acccgttacc aattaagagg gtatatcgaa
2220gatagtcaag acttagaaat ctatttaatt cgctacaatg ccaaacacga
aacagtaaat 2280gtgccaggta cgggttcctt atggccgctt tcagccccaa
gtccaatcgg aaaatgtgcc 2340catcattccc atcatttctc cttggacatt
gatgttggat gtacagactt aaatgaggac 2400ttaggtgtat gggtgatatt
caagattaag acgcaagatg gccatgcaag actaggaaat 2460ctagaatttc
tcgaagagaa accattagta ggagaagcac tagctcgtgt gaaaagagcg
2520gagaaaaaat ggagagacaa acgtgaaaaa ttggaatggg aaacaaatat
tgtttataaa 2580gaggcaaaag aatctgtaga tgctttattt gtaaactctc
aatatgatag attacaagcg 2640gataccaaca tcgcgatgat tcatgcggca
gataaacgcg ttcatagcat tcgagaagct 2700tatctgcctg agctgtctgt
gattccgggt gtcaatgcgg ctatttttga agaattagaa 2760gggcgtattt
tcactgcatt ctccctatat gatgcgagaa atgtcattaa aaatggtgat
2820tttaataatg gcttatcctg ctggaacgtg aaagggcatg tagatgtaga
agaacaaaac 2880aaccaccgtt cggtccttgt tgttccggaa tgggaagcag
aagtgtcaca agaagttcgt 2940gtctgtccgg gtcgtggcta tatccttcgt
gtcacagcgt acaaggaggg atatggagaa 3000ggttgcgtaa ccattcatga
gatcgagaac aatacagacg aactgaagtt tagcaactgt 3060gtagaagagg
aagtatatcc aaacaacacg gtaacgtgta atgattatac tgcgactcaa
3120gaagaatatg agggtacgta cacttctcgt aatcgaggat atgacggagc
ctatgaaagc 3180aattcttctg taccagctga ttatgcatca gcctatgaag
aaaaagcata tacagatgga 3240cgaagagaca atccttgtga atctaacaga
ggatatgggg attacacacc actaccagct 3300ggctatgtga caaaagaatt
agagtacttc ccagaaaccg ataaggtatg gattgagatc 3360ggagaaacgg
aaggaacatt catcgtggac agcgtggaat tacttcttat ggaggaatag
3420221139PRTArtificial sequenceAmino acid sequence of the
engineered insecticidal chimeric protein TIC844_6. 22Met Glu Ile
Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser1 5 10 15Asn Pro
Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 20 25 30Thr
Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 35 40
45Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp
50 55 60Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile
Glu65 70 75 80Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn
Gln Ala Ile 85 90 95Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val
Tyr Val Arg Ala 100 105 110Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn
Pro Ala Leu Arg Glu Glu 115 120 125Met Arg Ile Gln Phe Asn Asp Met
Asn Ser Ala Leu Ile Thr Ala Ile 130 135 140Pro Leu Phe Arg Val Gln
Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr145 150 155 160Val Gln Ala
Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 165 170 175Phe
Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 180 185
190Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp
195 200 205Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu
Ser Asp 210 215 220Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr
Ile Ser Val Leu225 230 235 240Asp Ile Val Ala Phe Phe Pro Asn Tyr
Asp Ile Arg Thr Tyr Pro Ile 245 250 255Gln Thr Ala Thr Gln Leu Thr
Arg Glu Val Tyr Leu Asp Leu Pro Phe 260 265 270Ile Asn Glu Asn Leu
Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser Ala 275 280 285Ala Glu Ser
Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 290 295 300Ser
Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Ala Tyr Trp Gly305 310
315 320Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu
Ile 325 330 335Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg
Pro Val Thr 340 345 350Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg
Thr Leu Ser Tyr Ile 355 360 365Thr Gly Leu Asp Asn Arg Asn Pro Val
Ala Gly Ile Glu Gly Val Glu 370 375 380Phe Gln Asn Thr Ile Ser Arg
Ser Ile Tyr Arg Lys Ser Gly Pro Ile385 390 395 400Asp Ser Phe Ser
Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 405 410 415Ile Gly
Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 420 425
430Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser
435 440 445Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln
Ile Pro 450 455 460Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser
Val Ile Lys Gly465 470 475 480Pro Gly Phe Thr Gly Gly Asp Ile Leu
Thr Arg Asn Ser Met Gly Glu 485 490 495Leu Gly Thr Leu Arg Val Thr
Phe Thr Gly Arg Leu Pro Gln Ser Tyr 500 505 510Tyr Ile Arg Phe Arg
Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 515 520 525Arg Tyr Ser
Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 530 535 540Asp
Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu545 550
555 560Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu
Tyr 565 570 575Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile
Pro Val Thr 580 585 590Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg
Ala Gln Lys Ala Val 595 600 605Asn Glu Leu Phe Thr Ser Ser Asn Gln
Ile Gly Leu Lys Thr Asp Val 610 615 620Thr Asp Tyr His Ile Asp Gln
Val Ser Asn Leu Val Glu Cys Leu Ser625 630 635 640Asp Glu Phe Cys
Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys 645 650 655His Ala
Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 660 665
670Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr
675 680 685Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn
Tyr Val 690 695 700Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr
Tyr Leu Tyr Gln705 710 715 720Lys Ile Asp Glu Ser Lys Leu Lys Ala
Tyr Thr Arg Tyr Gln Leu Arg 725 730 735Gly Tyr Ile Glu Asp Ser Gln
Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 740 745 750Asn Ala Lys His Glu
Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp 755 760 765Pro Leu Ser
Ala Pro Ser Pro Ile Gly Lys Cys Ala His His Ser His 770 775 780His
Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp785 790
795 800Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His
Ala 805 810 815Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu
Val Gly Glu 820 825 830Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys
Trp Arg Asp Lys Arg 835 840 845Glu Lys Leu Glu Trp Glu Thr Asn Ile
Val Tyr Lys Glu Ala Lys Glu 850 855 860Ser Val Asp Ala Leu Phe Val
Asn Ser Gln Tyr Asp Arg Leu Gln Ala865 870 875 880Asp Thr Asn Ile
Ala Met Ile His Ala Ala Asp Lys Arg Val His Ser 885 890 895Ile Arg
Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn 900 905
910Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile Phe Thr Ala Phe Ser
915 920 925Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn Gly Asp Phe Asn
Asn Gly 930 935 940Leu Ser Cys Trp Asn Val Lys Gly His Val Asp Val
Glu Glu Gln Asn945 950 955 960Asn His Arg Ser Val Leu Val Val Pro
Glu Trp Glu Ala Glu Val Ser 965 970 975Gln Glu Val Arg Val Cys Pro
Gly Arg Gly Tyr Ile Leu Arg Val Thr 980 985 990Ala Tyr Lys Glu Gly
Tyr Gly Glu Gly Cys Val Thr Ile His Glu Ile 995 1000 1005Glu Asn
Asn Thr Asp Glu Leu Lys Phe Ser Asn Cys Val Glu Glu 1010 1015
1020Glu Val Tyr Pro Asn Asn Thr Val Thr Cys Asn Asp Tyr Thr Ala
1025 1030 1035Thr Gln Glu Glu Tyr Glu Gly Thr Tyr Thr Ser Arg Asn
Arg Gly 1040 1045 1050Tyr Asp Gly Ala Tyr Glu Ser Asn Ser Ser Val
Pro Ala Asp Tyr 1055 1060 1065Ala Ser Ala Tyr Glu Glu Lys Ala Tyr
Thr Asp Gly Arg Arg Asp 1070 1075 1080Asn Pro Cys Glu Ser Asn Arg
Gly Tyr Gly Asp Tyr Thr Pro Leu 1085 1090 1095Pro Ala Gly Tyr Val
Thr Lys Glu Leu Glu Tyr Phe Pro Glu Thr 1100 1105 1110Asp Lys Val
Trp Ile Glu Ile Gly Glu Thr Glu Gly Thr Phe Ile 1115 1120 1125Val
Asp Ser Val Glu Leu Leu Leu Met Glu Glu 1130
1135233420DNAArtificial sequenceNucleotide sequence used for
expression in a bacterial cell encoding TIC844_7. 23atggaaataa
ataatcaaaa ccaatgtgtg ccttacaatt gtttaagtaa tcctaaggag 60ataatattag
gcgaggaaag gctagaaaca gggaatactg tagcagacat ttcattaggg
120cttattaatt ttctatattc taattttgta ccaggaggag gatttatagt
aggtttacta 180gaattaatat ggggatttat agggccttcg caatgggata
tttttttagc tcaaattgag 240caattgatta gtcaaagaat agaagaattt
gctaggaatc aggcaatttc aagattggag 300gggctaagca atctttataa
ggtctatgtt agagcgttta gcgactggga gaaagatcct 360actaatcctg
ctttaaggga agaaatgcgt atacaattta atgacatgaa tagtgctctc
420ataacggcta ttccactttt tagagttcaa aattatgaag ttgctctttt
atctgtatat 480gttcaagccg caaacttaca tttatctatt ttaagggatg
tttcagtttt cggagaaaga 540tggggatatg atacagcgac tatcaataat
cgctatagtg atctgactag ccttattcat 600gtttatacta accattgtgt
ggatacgtat aatcagggat taaggcgttt ggaaggtcgt 660tttcttagcg
attggattgt atataatcgt ttccggagac aattgacaat ttcagtatta
720gatattgttg cgttttttcc aaattatgat attagaacat atccaattca
aacagctact 780cagctaacga gggaagtcta tctggattta ccttttatta
atgaaaatct ttctcctgca 840gcaagctatc caaccttttc agctgctgaa
agtgctataa ttagaagtcc tcatttagta 900gactttttaa atagctttac
catttataca gatagtctgg cacgttctgc atattgggga 960gggcacttgg
taaattcttt ccgcacagga accactacta atttgataag atccccttta
1020tatggaaggg aaggaaatac agagcgcccc gtaactatta ccgcatcacc
tagcgtacca 1080atatttagaa cactttcata tagaacaggc cttgacaatt
caaatcctgt agctggaatc 1140gagggagtgg aattccaaaa tactataagt
agaagtatct atcgtaaaag cggtccaata 1200gattctttta gtgaattacc
acctcaagat gccagcgtat ctcctgcaat tgggtatagt 1260caccgtttat
gccatgcaac atttttagaa cggattagtg gaccaagaat agcaggcacc
1320gtattttctt ggacacaccg tagtgccagc cctactaacg aagtaagtcc
atctagaatt 1380acacaaattc catgggtaaa ggcgcatact cttgcatctg
gtgcctccgt cattaaaggt 1440cctggattta caggtggaga tattctgact
aggaatagta tgggcgagct ggggacctta 1500cgagtaacct tcacaggaag
attaccacaa agttattata tacgtttccg ttatgcttcg 1560gtagcaaata
ggagtggtac atttagatat tcacagccac cttcgtatgg aatttcattt
1620ccaaaaacta tggacgcagg tgaaccacta acatctcgtt cgttcgctca
tacaacactc 1680ttcactccaa taaccttttc acgagctcaa gaagaatttg
atctatacat ccaatcgggt 1740gtttatatag atcgaattga atttataccg
gttactgcaa catttgaggc agaatatgat 1800ttagaaagag cacaaaaggc
ggtgaatgag ctgtttactt cttccaatca aatcgggtta 1860aaaacagatg
tgacggatta tcatattgat caagtatcca atttagttga gtgtttatct
1920gatgaatttt gtctggatga aaaaaaagaa ttgtccgaga aagtcaaaca
tgcgaagcga 1980cttagtgatg agcggaattt acttcaagat ccaaacttta
gagggatcaa tagacaacta 2040gaccgtggct ggagaggaag tacggatatt
accatccaag gaggcgatga cgtattcaaa 2100gagaattacg ttacgctatt
gggtaccttt gatgagtgct atccaacgta tttatatcaa 2160aaaatagatg
agtcgaaatt aaaagcctat acccgttacc aattaagagg gtatatcgaa
2220gatagtcaag acttagaaat ctatttaatt cgctacaatg ccaaacacga
aacagtaaat 2280gtgccaggta cgggttcctt atggccgctt tcagccccaa
gtccaatcgg aaaatgtgcc 2340catcattccc atcatttctc cttggacatt
gatgttggat gtacagactt aaatgaggac 2400ttaggtgtat gggtgatatt
caagattaag acgcaagatg gccatgcaag actaggaaat 2460ctagaatttc
tcgaagagaa accattagta ggagaagcac tagctcgtgt gaaaagagcg
2520gagaaaaaat ggagagacaa
acgtgaaaaa ttggaatggg aaacaaatat tgtttataaa 2580gaggcaaaag
aatctgtaga tgctttattt gtaaactctc aatatgatag attacaagcg
2640gataccaaca tcgcgatgat tcatgcggca gataaacgcg ttcatagcat
tcgagaagct 2700tatctgcctg agctgtctgt gattccgggt gtcaatgcgg
ctatttttga agaattagaa 2760gggcgtattt tcactgcatt ctccctatat
gatgcgagaa atgtcattaa aaatggtgat 2820tttaataatg gcttatcctg
ctggaacgtg aaagggcatg tagatgtaga agaacaaaac 2880aaccaccgtt
cggtccttgt tgttccggaa tgggaagcag aagtgtcaca agaagttcgt
2940gtctgtccgg gtcgtggcta tatccttcgt gtcacagcgt acaaggaggg
atatggagaa 3000ggttgcgtaa ccattcatga gatcgagaac aatacagacg
aactgaagtt tagcaactgt 3060gtagaagagg aagtatatcc aaacaacacg
gtaacgtgta atgattatac tgcgactcaa 3120gaagaatatg agggtacgta
cacttctcgt aatcgaggat atgacggagc ctatgaaagc 3180aattcttctg
taccagctga ttatgcatca gcctatgaag aaaaagcata tacagatgga
3240cgaagagaca atccttgtga atctaacaga ggatatgggg attacacacc
actaccagct 3300ggctatgtga caaaagaatt agagtacttc ccagaaaccg
ataaggtatg gattgagatc 3360ggagaaacgg aaggaacatt catcgtggac
agcgtggaat tacttcttat ggaggaatag 3420241139PRTArtificial
sequenceAmino acid sequence of the engineered insecticidal chimeric
protein TIC844_7. 24Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr
Asn Cys Leu Ser1 5 10 15Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg
Leu Glu Thr Gly Asn 20 25 30Thr Val Ala Asp Ile Ser Leu Gly Leu Ile
Asn Phe Leu Tyr Ser Asn 35 40 45Phe Val Pro Gly Gly Gly Phe Ile Val
Gly Leu Leu Glu Leu Ile Trp 50 55 60Gly Phe Ile Gly Pro Ser Gln Trp
Asp Ile Phe Leu Ala Gln Ile Glu65 70 75 80Gln Leu Ile Ser Gln Arg
Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 85 90 95Ser Arg Leu Glu Gly
Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 100 105 110Phe Ser Asp
Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 115 120 125Met
Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 130 135
140Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val
Tyr145 150 155 160Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg
Asp Val Ser Val 165 170 175Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala
Thr Ile Asn Asn Arg Tyr 180 185 190Ser Asp Leu Thr Ser Leu Ile His
Val Tyr Thr Asn His Cys Val Asp 195 200 205Thr Tyr Asn Gln Gly Leu
Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 210 215 220Trp Ile Val Tyr
Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu225 230 235 240Asp
Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 245 250
255Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe
260 265 270Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe
Ser Ala 275 280 285Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val
Asp Phe Leu Asn 290 295 300Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala
Arg Ser Ala Tyr Trp Gly305 310 315 320Gly His Leu Val Asn Ser Phe
Arg Thr Gly Thr Thr Thr Asn Leu Ile 325 330 335Arg Ser Pro Leu Tyr
Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 340 345 350Ile Thr Ala
Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Arg 355 360 365Thr
Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 370 375
380Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro
Ile385 390 395 400Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser
Val Ser Pro Ala 405 410 415Ile Gly Tyr Ser His Arg Leu Cys His Ala
Thr Phe Leu Glu Arg Ile 420 425 430Ser Gly Pro Arg Ile Ala Gly Thr
Val Phe Ser Trp Thr His Arg Ser 435 440 445Ala Ser Pro Thr Asn Glu
Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 450 455 460Trp Val Lys Ala
His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly465 470 475 480Pro
Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 485 490
495Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr
500 505 510Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly
Thr Phe 515 520 525Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe
Pro Lys Thr Met 530 535 540Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser
Phe Ala His Thr Thr Leu545 550 555 560Phe Thr Pro Ile Thr Phe Ser
Arg Ala Gln Glu Glu Phe Asp Leu Tyr 565 570 575Ile Gln Ser Gly Val
Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 580 585 590Ala Thr Phe
Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val 595 600 605Asn
Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val 610 615
620Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu
Ser625 630 635 640Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser
Glu Lys Val Lys 645 650 655His Ala Lys Arg Leu Ser Asp Glu Arg Asn
Leu Leu Gln Asp Pro Asn 660 665 670Phe Arg Gly Ile Asn Arg Gln Leu
Asp Arg Gly Trp Arg Gly Ser Thr 675 680 685Asp Ile Thr Ile Gln Gly
Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 690 695 700Thr Leu Leu Gly
Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln705 710 715 720Lys
Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 725 730
735Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr
740 745 750Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser
Leu Trp 755 760 765Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Ala
His His Ser His 770 775 780His Phe Ser Leu Asp Ile Asp Val Gly Cys
Thr Asp Leu Asn Glu Asp785 790 795 800Leu Gly Val Trp Val Ile Phe
Lys Ile Lys Thr Gln Asp Gly His Ala 805 810 815Arg Leu Gly Asn Leu
Glu Phe Leu Glu Glu Lys Pro Leu Val Gly Glu 820 825 830Ala Leu Ala
Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg 835 840 845Glu
Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu 850 855
860Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln
Ala865 870 875 880Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys
Arg Val His Ser 885 890 895Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser
Val Ile Pro Gly Val Asn 900 905 910Ala Ala Ile Phe Glu Glu Leu Glu
Gly Arg Ile Phe Thr Ala Phe Ser 915 920 925Leu Tyr Asp Ala Arg Asn
Val Ile Lys Asn Gly Asp Phe Asn Asn Gly 930 935 940Leu Ser Cys Trp
Asn Val Lys Gly His Val Asp Val Glu Glu Gln Asn945 950 955 960Asn
His Arg Ser Val Leu Val Val Pro Glu Trp Glu Ala Glu Val Ser 965 970
975Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr
980 985 990Ala Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile His
Glu Ile 995 1000 1005Glu Asn Asn Thr Asp Glu Leu Lys Phe Ser Asn
Cys Val Glu Glu 1010 1015 1020Glu Val Tyr Pro Asn Asn Thr Val Thr
Cys Asn Asp Tyr Thr Ala 1025 1030 1035Thr Gln Glu Glu Tyr Glu Gly
Thr Tyr Thr Ser Arg Asn Arg Gly 1040 1045 1050Tyr Asp Gly Ala Tyr
Glu Ser Asn Ser Ser Val Pro Ala Asp Tyr 1055 1060 1065Ala Ser Ala
Tyr Glu Glu Lys Ala Tyr Thr Asp Gly Arg Arg Asp 1070 1075 1080Asn
Pro Cys Glu Ser Asn Arg Gly Tyr Gly Asp Tyr Thr Pro Leu 1085 1090
1095Pro Ala Gly Tyr Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu Thr
1100 1105 1110Asp Lys Val Trp Ile Glu Ile Gly Glu Thr Glu Gly Thr
Phe Ile 1115 1120 1125Val Asp Ser Val Glu Leu Leu Leu Met Glu Glu
1130 1135253420DNAArtificial sequenceNucleotide sequence used for
expression in a bacterial cell encoding TIC844_8. 25atggaaataa
ataatcaaaa ccaatgtgtg ccttacaatt gtttaagtaa tcctaaggag 60ataatattag
gcgaggaaag gctagaaaca gggaatactg tagcagacat ttcattaggg
120cttattaatt ttctatattc taattttgta ccaggaggag gatttatagt
aggtttacta 180gaattaatat ggggatttat agggccttcg caatgggata
tttttttagc tcaaattgag 240caattgatta gtcaaagaat agaagaattt
gctaggaatc aggcaatttc aagattggag 300gggctaagca atctttataa
ggtctatgtt agagcgttta gcgactggga gaaagatcct 360actaatcctg
ctttaaggga agaaatgcgt atacaattta atgacatgaa tagtgctctc
420ataacggcta ttccactttt tagagttcaa aattatgaag ttgctctttt
atctgtatat 480gttcaagccg caaacttaca tttatctatt ttaagggatg
tttcagtttt cggagaaaga 540tggggatatg atacagcgac tatcaataat
cgctatagtg atctgactag ccttattcat 600gtttatacta accattgtgt
ggatacgtat aatcagggat taaggcgttt ggaaggtcgt 660tttcttagcg
attggattgt atataatcgt ttccggagac aattgacaat ttcagtatta
720gatattgttg cgttttttcc aaattatgat attagaacat atccaattca
aacagctact 780cagctaacga gggaagtcta tctggattta ccttttatta
atgaaaatct ttctcctgca 840gcagtatatc caaccttttc agctgctgaa
agtgctataa ttagaagtcc tcatttagta 900gactttttaa atagctttac
catttataca gatagtctgg cacgttctgc atattgggga 960gggcacttgg
taaattcttt ccgcacagga accactacta atttgataag atccccttta
1020tatggaaggg aaggaaatac agagcgcccc gtaactatta ccgcatcacc
tagcgtacca 1080atatttagaa cactttcata tccaacaggc cttgacaatt
caaatcctgt agctggaatc 1140gagggagtgg aattccaaaa tactataagt
agaagtatct atcgtaaaag cggtccaata 1200gattctttta gtgaattacc
acctcaagat gccagcgtat ctcctgcaat tgggtatagt 1260caccgtttat
gccatgcaac atttttagaa cggattagtg gaccaagaat agcaggcacc
1320gtattttctt ggacacaccg tagtgccagc cctactaacg aagtaagtcc
atctagaatt 1380acacaaattc catgggtaaa ggcgcatact cttgcatctg
gtgcctccgt cattaaaggt 1440cctggattta caggtggaga tattctgact
aggaatagta tgggcgagct ggggacctta 1500cgagtaacct tcacaggaag
attaccacaa agttattata tacgtttccg ttatgcttcg 1560gtagcaaata
ggagtggtac atttagatat tcacagccac cttcgtatgg aatttcattt
1620ccaaaaacta tggacgcagg tgaaccacta acatctcgtt cgttcgctca
tacaacactc 1680ttcactccaa taaccttttc acgagctcaa gaagaatttg
atctatacat ccaatcgggt 1740gtttatatag atcgaattga atttataccg
gttactgcaa catttgaggc agaatatgat 1800ttagaaagag cacaaaaggc
ggtgaatgag ctgtttactt cttccaatca aatcgggtta 1860aaaacagatg
tgacggatta tcatattgat caagtatcca atttagttga gtgtttatct
1920gatgaatttt gtctggatga aaaaaaagaa ttgtccgaga aagtcaaaca
tgcgaagcga 1980cttagtgatg agcggaattt acttcaagat ccaaacttta
gagggatcaa tagacaacta 2040gaccgtggct ggagaggaag tacggatatt
accatccaag gaggcgatga cgtattcaaa 2100gagaattacg ttacgctatt
gggtaccttt gatgagtgct atccaacgta tttatatcaa 2160aaaatagatg
agtcgaaatt aaaagcctat acccgttacc aattaagagg gtatatcgaa
2220gatagtcaag acttagaaat ctatttaatt cgctacaatg ccaaacacga
aacagtaaat 2280gtgccaggta cgggttcctt atggccgctt tcagccccaa
gtccaatcgg aaaatgtgcc 2340catcattccc atcatttctc cttggacatt
gatgttggat gtacagactt aaatgaggac 2400ttaggtgtat gggtgatatt
caagattaag acgcaagatg gccatgcaag actaggaaat 2460ctagaatttc
tcgaagagaa accattagta ggagaagcac tagctcgtgt gaaaagagcg
2520gagaaaaaat ggagagacaa acgtgaaaaa ttggaatggg aaacaaatat
tgtttataaa 2580gaggcaaaag aatctgtaga tgctttattt gtaaactctc
aatatgatag attacaagcg 2640gataccaaca tcgcgatgat tcatgcggca
gataaacgcg ttcatagcat tcgagaagct 2700tatctgcctg agctgtctgt
gattccgggt gtcaatgcgg ctatttttga agaattagaa 2760gggcgtattt
tcactgcatt ctccctatat gatgcgagaa atgtcattaa aaatggtgat
2820tttaataatg gcttatcctg ctggaacgtg aaagggcatg tagatgtaga
agaacaaaac 2880aaccaccgtt cggtccttgt tgttccggaa tgggaagcag
aagtgtcaca agaagttcgt 2940gtctgtccgg gtcgtggcta tatccttcgt
gtcacagcgt acaaggaggg atatggagaa 3000ggttgcgtaa ccattcatga
gatcgagaac aatacagacg aactgaagtt tagcaactgt 3060gtagaagagg
aagtatatcc aaacaacacg gtaacgtgta atgattatac tgcgactcaa
3120gaagaatatg agggtacgta cacttctcgt aatcgaggat atgacggagc
ctatgaaagc 3180aattcttctg taccagctga ttatgcatca gcctatgaag
aaaaagcata tacagatgga 3240cgaagagaca atccttgtga atctaacaga
ggatatgggg attacacacc actaccagct 3300ggctatgtga caaaagaatt
agagtacttc ccagaaaccg ataaggtatg gattgagatc 3360ggagaaacgg
aaggaacatt catcgtggac agcgtggaat tacttcttat ggaggaatag
3420261139PRTArtificial sequenceAmino acid sequence of the
engineered insecticidal chimeric protein TIC844_8. 26Met Glu Ile
Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser1 5 10 15Asn Pro
Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 20 25 30Thr
Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 35 40
45Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp
50 55 60Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile
Glu65 70 75 80Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn
Gln Ala Ile 85 90 95Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val
Tyr Val Arg Ala 100 105 110Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn
Pro Ala Leu Arg Glu Glu 115 120 125Met Arg Ile Gln Phe Asn Asp Met
Asn Ser Ala Leu Ile Thr Ala Ile 130 135 140Pro Leu Phe Arg Val Gln
Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr145 150 155 160Val Gln Ala
Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 165 170 175Phe
Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 180 185
190Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp
195 200 205Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu
Ser Asp 210 215 220Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr
Ile Ser Val Leu225 230 235 240Asp Ile Val Ala Phe Phe Pro Asn Tyr
Asp Ile Arg Thr Tyr Pro Ile 245 250 255Gln Thr Ala Thr Gln Leu Thr
Arg Glu Val Tyr Leu Asp Leu Pro Phe 260 265 270Ile Asn Glu Asn Leu
Ser Pro Ala Ala Val Tyr Pro Thr Phe Ser Ala 275 280 285Ala Glu Ser
Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 290 295 300Ser
Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp Gly305 310
315 320Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu
Ile 325 330 335Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg
Pro Val Thr 340 345 350Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg
Thr Leu Ser Tyr Pro 355 360 365Thr Gly Leu Asp Asn Ser Asn Pro Val
Ala Gly Ile Glu Gly Val Glu 370 375 380Phe Gln Asn Thr Ile Ser Arg
Ser Ile Tyr Arg Lys Ser Gly Pro Ile385 390 395 400Asp Ser Phe Ser
Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 405 410 415Ile Gly
Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 420 425
430Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser
435 440 445Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln
Ile Pro 450 455 460Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser
Val Ile Lys Gly465 470 475 480Pro Gly Phe Thr Gly Gly Asp Ile Leu
Thr Arg Asn Ser Met Gly Glu 485 490 495Leu Gly Thr Leu Arg Val Thr
Phe Thr Gly Arg Leu Pro Gln Ser Tyr 500 505 510Tyr Ile Arg Phe Arg
Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 515 520 525Arg Tyr Ser
Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 530 535 540Asp
Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu545 550
555 560Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu
Tyr 565 570 575Ile Gln Ser Gly Val
Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 580 585 590Ala Thr Phe
Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val 595 600 605Asn
Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val 610 615
620Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu
Ser625 630 635 640Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser
Glu Lys Val Lys 645 650 655His Ala Lys Arg Leu Ser Asp Glu Arg Asn
Leu Leu Gln Asp Pro Asn 660 665 670Phe Arg Gly Ile Asn Arg Gln Leu
Asp Arg Gly Trp Arg Gly Ser Thr 675 680 685Asp Ile Thr Ile Gln Gly
Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 690 695 700Thr Leu Leu Gly
Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln705 710 715 720Lys
Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 725 730
735Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr
740 745 750Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser
Leu Trp 755 760 765Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Ala
His His Ser His 770 775 780His Phe Ser Leu Asp Ile Asp Val Gly Cys
Thr Asp Leu Asn Glu Asp785 790 795 800Leu Gly Val Trp Val Ile Phe
Lys Ile Lys Thr Gln Asp Gly His Ala 805 810 815Arg Leu Gly Asn Leu
Glu Phe Leu Glu Glu Lys Pro Leu Val Gly Glu 820 825 830Ala Leu Ala
Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg 835 840 845Glu
Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu 850 855
860Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln
Ala865 870 875 880Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys
Arg Val His Ser 885 890 895Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser
Val Ile Pro Gly Val Asn 900 905 910Ala Ala Ile Phe Glu Glu Leu Glu
Gly Arg Ile Phe Thr Ala Phe Ser 915 920 925Leu Tyr Asp Ala Arg Asn
Val Ile Lys Asn Gly Asp Phe Asn Asn Gly 930 935 940Leu Ser Cys Trp
Asn Val Lys Gly His Val Asp Val Glu Glu Gln Asn945 950 955 960Asn
His Arg Ser Val Leu Val Val Pro Glu Trp Glu Ala Glu Val Ser 965 970
975Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr
980 985 990Ala Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile His
Glu Ile 995 1000 1005Glu Asn Asn Thr Asp Glu Leu Lys Phe Ser Asn
Cys Val Glu Glu 1010 1015 1020Glu Val Tyr Pro Asn Asn Thr Val Thr
Cys Asn Asp Tyr Thr Ala 1025 1030 1035Thr Gln Glu Glu Tyr Glu Gly
Thr Tyr Thr Ser Arg Asn Arg Gly 1040 1045 1050Tyr Asp Gly Ala Tyr
Glu Ser Asn Ser Ser Val Pro Ala Asp Tyr 1055 1060 1065Ala Ser Ala
Tyr Glu Glu Lys Ala Tyr Thr Asp Gly Arg Arg Asp 1070 1075 1080Asn
Pro Cys Glu Ser Asn Arg Gly Tyr Gly Asp Tyr Thr Pro Leu 1085 1090
1095Pro Ala Gly Tyr Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu Thr
1100 1105 1110Asp Lys Val Trp Ile Glu Ile Gly Glu Thr Glu Gly Thr
Phe Ile 1115 1120 1125Val Asp Ser Val Glu Leu Leu Leu Met Glu Glu
1130 1135273498DNAArtificial sequenceSynthetic DNA sequence
designed for plant expression encoding Cry1Da1. 27atggagatca
acaaccagaa ccagtgcgtc ccgtacaact gcctgagcaa ccctaaggag 60atcatcctgg
gtgaggaacg cctggagacc ggcaacaccg tagccgacat tagcctgggc
120ctcatcaact tcctctacag caacttcgtg cccggcggtg gcttcatcgt
gggcctcctg 180gagcttatct ggggcttcat cggcccgtcc cagtgggaca
tcttcctcgc ccagatcgag 240caactgatca gccagcggat cgaggagttc
gctaggaacc aggccatctc ccgcctggag 300ggactctcca acctctacaa
ggtgtacgtg cgcgcgttca gcgactggga gaaggacccg 360accaacccgg
ccctccgcga ggaaatgcgt atccagttca acgatatgaa ctcggccctc
420atcaccgcca tcccgctctt ccgcgtgcag aactacgagg tggccctcct
gtccgtgtac 480gttcaagccg ccaacctcca cctctccatc ctccgcgacg
tgagcgtgtt cggcgagcgc 540tggggctacg acaccgccac catcaacaac
cgctactccg acctcacctc cctcatccac 600gtttacacca accactgcgt
ggacacgtac aaccagggcc tccgccgcct ggagggccgc 660ttcctctccg
actggatcgt gtacaaccgc ttccgccgcc agctcaccat ctccgtcctg
720gacatcgtcg ccttctttcc caactacgac atccgcacct accctatcca
gaccgccacc 780cagctcaccc gcgaggtcta cctcgacctc ccgttcatca
acgagaacct cagcccggcc 840gccagctacc cgaccttctc cgccgctgag
tccgccatca ttcgcagccc gcacctcgtg 900gacttcctca actccttcac
catctacacc gactccctcg cccgctacgc ctactggggc 960ggtcacctcg
tgaactcctt ccgcaccggc accactacca acctcatccg cagcccgctc
1020tacggccgcg agggcaacac cgagcgcccg gtgaccatca ccgccagccc
gagcgtgccc 1080atcttccgca ccctcagcta catcaccggc ctggacaaca
gcaaccctgt ggcgggcatc 1140gagggcgtgg agttccagaa caccatctcc
aggagcatct accgcaagag cggccctatc 1200gacagcttca gcgagctgcc
tcctcaggac gccagcgtga gccctgccat cggctacagc 1260cacaggctgt
gccacgccac cttcctggag cgcatcagcg gccctcgcat cgcgggcacc
1320gtgttctcgt ggacccaccg cagcgcctct cctacgaacg aggtgtctcc
tagtcgcatc 1380acccagatcc cttgggtcaa ggcccacacc ctggctagtg
gcgctagtgt catcaagggc 1440cctggcttca ccggtggtga catcctgacc
aggaactcta tgggcgagct gggcactctg 1500agggtcactt tcactggccg
cctgcctcag tcttactaca tccgcttccg ctacgctagt 1560gtcgctaacc
gctctggtac tttccgctac tctcagcctc cgtcttacgg tatctctttc
1620cctaagacta tggacgctgg tgagcctctg accagtagga gcttcgctca
cactactctg 1680ttcactccta tcactttctc tagggctcag gaggagttcg
acctgtacat ccagtctggt 1740gtgtacatcg acaggatcga gttcatcccc
gtgaccgcca cgttcgaggc cgagtacgac 1800cttgagcgcg cccagaaggt
ggtgaacgcc ctcttcacta gcactaacca gctaggcctg 1860aagactgacg
tgaccgacta ccacatcgac caagtgagca acctagtggc ctgcctctcc
1920gacgagttct gcctcgacga gaagcgcgag ctgtccgaga aggtgaagca
cgccaagcgc 1980ctctccgacg agcgcaacct gctccaggac cccaacttca
ggggcatcaa caggcagccc 2040gaccgcggct ggcgcggctc caccgacatc
accatccagg gcggtgacga cgtattcaag 2100gagaactacg ttaccctccc
cggcaccttc gacgagtgtt accccaccta cctctaccag 2160aagatcgacg
agtccaagct gaaggcctac acccgctacc agctccgcgg ctacatcgag
2220gactcccagg acctggaaat ctacctcatc cgctacaacg ccaagcacga
gatcgtgaac 2280gtgcctggca ccggcagcct ctggcctctc agcgtggaga
accagatcgg cccttgcggc 2340gagcctaacc gctgcgcccc tcacctcgag
tggaaccctg acctccactg ctcgtgcagg 2400gacggcgaga agtgcgccca
ccatagccac cacttctctc tggacatcga cgtgggctgc 2460accgacctga
acgaggacct gggcgtgtgg gttatcttca agatcaagac ccaggacggt
2520cacgccaggc tgggtaacct ggagttcctt gaggaaaagc ctctgctggg
tgaggccctg 2580gccagggtca agagggctga gaagaaatgg agggataaga
gggagaccct gcagctggag 2640accactatcg tctacaagga ggctaaggag
tctgtcgatg ctctgttcgt caactctcag 2700tacgatagac tgcaagctga
taccaacatc gctatgatcc acgctgcgga taagcgggtc 2760caccggatcc
gggaggctta ccttccggag ctttctgtca tcccgggtgt caacgctgcg
2820atcttcgagg aacttgagga acggatcttc actgcgttta gtctttacga
tgcgcggaac 2880atcatcaaga acggggactt caacaatggt ctgctgtgct
ggaacgtcaa gggtcatgtc 2940gaggtcgagg aacaaaacaa tcatcgtagt
gtccttgtca ttcctgagtg ggaggcggag 3000gtctctcaag aggtccgtgt
ttgcccgggg cgtgggtaca ttcttcgtgt tactgcgtac 3060aaggaggggt
acggggaggg gtgcgttact attcatgaga ttgagaacaa tactgatgag
3120cttaagttca acaattgtgt tgaggaggag gtttacccga acaatactgt
tacgtgcatc 3180aactacacgg caacgcaaga ggaatacgag gggacgtaca
cctcgcgtaa tagagggtat 3240gatgaggcgt acggaaacaa cccgtcggtt
ccagcagatt atgcctcggt ttatgaggag 3300aagtcgtaca cggatagacg
acgcgagaat ccatgtgagt caaatcgagg atacggagat 3360tacacaccat
taccagcagg atacgttaca aaggagttgg aatacttccc ggaaacagat
3420aaagtttgga ttgaaatcgg agaaacagaa ggaacattca tcgtcgactc
agtagaattg 3480ttgttgatgg aagaatga 3498281165PRTArtificial
sequenceAmino acid sequence of Cry1Da1 encoded by a synthetic DNA
sequence. 28Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys
Leu Ser1 5 10 15Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu
Thr Gly Asn 20 25 30Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe
Leu Tyr Ser Asn 35 40 45Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu
Leu Glu Leu Ile Trp 50 55 60Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile
Phe Leu Ala Gln Ile Glu65 70 75 80Gln Leu Ile Ser Gln Arg Ile Glu
Glu Phe Ala Arg Asn Gln Ala Ile 85 90 95Ser Arg Leu Glu Gly Leu Ser
Asn Leu Tyr Lys Val Tyr Val Arg Ala 100 105 110Phe Ser Asp Trp Glu
Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 115 120 125Met Arg Ile
Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 130 135 140Pro
Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr145 150
155 160Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser
Val 165 170 175Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn
Asn Arg Tyr 180 185 190Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr
Asn His Cys Val Asp 195 200 205Thr Tyr Asn Gln Gly Leu Arg Arg Leu
Glu Gly Arg Phe Leu Ser Asp 210 215 220Trp Ile Val Tyr Asn Arg Phe
Arg Arg Gln Leu Thr Ile Ser Val Leu225 230 235 240Asp Ile Val Ala
Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 245 250 255Gln Thr
Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 260 265
270Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser Ala
275 280 285Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe
Leu Asn 290 295 300Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr
Ala Tyr Trp Gly305 310 315 320Gly His Leu Val Asn Ser Phe Arg Thr
Gly Thr Thr Thr Asn Leu Ile 325 330 335Arg Ser Pro Leu Tyr Gly Arg
Glu Gly Asn Thr Glu Arg Pro Val Thr 340 345 350Ile Thr Ala Ser Pro
Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Ile 355 360 365Thr Gly Leu
Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 370 375 380Phe
Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile385 390
395 400Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro
Ala 405 410 415Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu
Glu Arg Ile 420 425 430Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser
Trp Thr His Arg Ser 435 440 445Ala Ser Pro Thr Asn Glu Val Ser Pro
Ser Arg Ile Thr Gln Ile Pro 450 455 460Trp Val Lys Ala His Thr Leu
Ala Ser Gly Ala Ser Val Ile Lys Gly465 470 475 480Pro Gly Phe Thr
Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 485 490 495Leu Gly
Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 500 505
510Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe
515 520 525Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys
Thr Met 530 535 540Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala
His Thr Thr Leu545 550 555 560Phe Thr Pro Ile Thr Phe Ser Arg Ala
Gln Glu Glu Phe Asp Leu Tyr 565 570 575Ile Gln Ser Gly Val Tyr Ile
Asp Arg Ile Glu Phe Ile Pro Val Thr 580 585 590Ala Thr Phe Glu Ala
Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val Val 595 600 605Asn Ala Leu
Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp Val 610 615 620Thr
Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu Ser625 630
635 640Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val
Lys 645 650 655His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln
Asp Pro Asn 660 665 670Phe Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly
Trp Arg Gly Ser Thr 675 680 685Asp Ile Thr Ile Gln Gly Gly Asp Asp
Val Phe Lys Glu Asn Tyr Val 690 695 700Thr Leu Pro Gly Thr Phe Asp
Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln705 710 715 720Lys Ile Asp Glu
Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 725 730 735Gly Tyr
Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 740 745
750Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser Leu Trp
755 760 765Pro Leu Ser Val Glu Asn Gln Ile Gly Pro Cys Gly Glu Pro
Asn Arg 770 775 780Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu His
Cys Ser Cys Arg785 790 795 800Asp Gly Glu Lys Cys Ala His His Ser
His His Phe Ser Leu Asp Ile 805 810 815Asp Val Gly Cys Thr Asp Leu
Asn Glu Asp Leu Gly Val Trp Val Ile 820 825 830Phe Lys Ile Lys Thr
Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu 835 840 845Phe Leu Glu
Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val Lys 850 855 860Arg
Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln Leu Glu865 870
875 880Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu
Phe 885 890 895Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn
Ile Ala Met 900 905 910Ile His Ala Ala Asp Lys Arg Val His Arg Ile
Arg Glu Ala Tyr Leu 915 920 925Pro Glu Leu Ser Val Ile Pro Gly Val
Asn Ala Ala Ile Phe Glu Glu 930 935 940Leu Glu Glu Arg Ile Phe Thr
Ala Phe Ser Leu Tyr Asp Ala Arg Asn945 950 955 960Ile Ile Lys Asn
Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn Val 965 970 975Lys Gly
His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser Val Leu 980 985
990Val Ile Pro Glu Trp Glu Ala Glu Val Ser Gln Glu Val Arg Val Cys
995 1000 1005Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr Ala Tyr Lys
Glu Gly 1010 1015 1020Tyr Gly Glu Gly Cys Val Thr Ile His Glu Ile
Glu Asn Asn Thr 1025 1030 1035Asp Glu Leu Lys Phe Asn Asn Cys Val
Glu Glu Glu Val Tyr Pro 1040 1045 1050Asn Asn Thr Val Thr Cys Ile
Asn Tyr Thr Ala Thr Gln Glu Glu 1055 1060 1065Tyr Glu Gly Thr Tyr
Thr Ser Arg Asn Arg Gly Tyr Asp Glu Ala 1070 1075 1080Tyr Gly Asn
Asn Pro Ser Val Pro Ala Asp Tyr Ala Ser Val Tyr 1085 1090 1095Glu
Glu Lys Ser Tyr Thr Asp Arg Arg Arg Glu Asn Pro Cys Glu 1100 1105
1110Ser Asn Arg Gly Tyr Gly Asp Tyr Thr Pro Leu Pro Ala Gly Tyr
1115 1120 1125Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu Thr Asp Lys
Val Trp 1130 1135 1140Ile Glu Ile Gly Glu Thr Glu Gly Thr Phe Ile
Val Asp Ser Val 1145 1150 1155Glu Leu Leu Leu Met Glu Glu 1160
1165293501DNAArtificial sequenceSynthetic DNA sequence designed for
plant expression encoding Cry1Da1 with an additional Alanine
residue inserted at position 2. 29atggctgaga tcaacaacca gaaccagtgc
gtcccgtaca actgcctgag caaccctaag 60gagatcatcc tgggtgagga acgcctggag
accggcaaca ccgtagccga cattagcctg 120ggcctcatca acttcctcta
cagcaacttc gtgcccggcg gtggcttcat cgtgggcctc 180ctggagctta
tctggggctt catcggcccg tcccagtggg acatcttcct cgcccagatc
240gagcaactga tcagccagcg gatcgaggag ttcgctagga accaggccat
ctcccgcctg 300gagggactct ccaacctcta caaggtgtac gtgcgcgcgt
tcagcgactg ggagaaggac 360ccgaccaacc cggccctccg cgaggaaatg
cgtatccagt tcaacgatat gaactcggcc 420ctcatcaccg ccatcccgct
cttccgcgtg cagaactacg aggtggccct cctgtccgtg 480tacgttcaag
ccgccaacct ccacctctcc atcctccgcg acgtgagcgt gttcggcgag
540cgctggggct acgacaccgc caccatcaac aaccgctact ccgacctcac
ctccctcatc 600cacgtttaca ccaaccactg cgtggacacg tacaaccagg
gcctccgccg cctggagggc 660cgcttcctct
ccgactggat cgtgtacaac cgcttccgcc gccagctcac catctccgtc
720ctggacatcg tcgccttctt tcccaactac gacatccgca cctaccctat
ccagaccgcc 780acccagctca cccgcgaggt ctacctcgac ctcccgttca
tcaacgagaa cctcagcccg 840gccgccagct acccgacctt ctccgccgct
gagtccgcca tcattcgcag cccgcacctc 900gtggacttcc tcaactcctt
caccatctac accgactccc tcgcccgcta cgcctactgg 960ggcggtcacc
tcgtgaactc cttccgcacc ggcaccacta ccaacctcat ccgcagcccg
1020ctctacggcc gcgagggcaa caccgagcgc ccggtgacca tcaccgccag
cccgagcgtg 1080cccatcttcc gcaccctcag ctacatcacc ggcctggaca
acagcaaccc tgtggcgggc 1140atcgagggcg tggagttcca gaacaccatc
tccaggagca tctaccgcaa gagcggccct 1200atcgacagct tcagcgagct
gcctcctcag gacgccagcg tgagccctgc catcggctac 1260agccacaggc
tgtgccacgc caccttcctg gagcgcatca gcggccctcg catcgcgggc
1320accgtgttct cgtggaccca ccgcagcgcc tctcctacga acgaggtgtc
tcctagtcgc 1380atcacccaga tcccttgggt caaggcccac accctggcta
gtggcgctag tgtcatcaag 1440ggccctggct tcaccggtgg tgacatcctg
accaggaact ctatgggcga gctgggcact 1500ctgagggtca ctttcactgg
ccgcctgcct cagtcttact acatccgctt ccgctacgct 1560agtgtcgcta
accgctctgg tactttccgc tactctcagc ctccgtctta cggtatctct
1620ttccctaaga ctatggacgc tggtgagcct ctgaccagta ggagcttcgc
tcacactact 1680ctgttcactc ctatcacttt ctctagggct caggaggagt
tcgacctgta catccagtct 1740ggtgtgtaca tcgacaggat cgagttcatc
cccgtgaccg ccacgttcga ggccgagtac 1800gaccttgagc gcgcccagaa
ggtggtgaac gccctcttca ctagcactaa ccagctaggc 1860ctgaagactg
acgtgaccga ctaccacatc gaccaagtga gcaacctagt ggcctgcctc
1920tccgacgagt tctgcctcga cgagaagcgc gagctgtccg agaaggtgaa
gcacgccaag 1980cgcctctccg acgagcgcaa cctgctccag gaccccaact
tcaggggcat caacaggcag 2040cccgaccgcg gctggcgcgg ctccaccgac
atcaccatcc agggcggtga cgacgtattc 2100aaggagaact acgttaccct
ccccggcacc ttcgacgagt gttaccccac ctacctctac 2160cagaagatcg
acgagtccaa gctgaaggcc tacacccgct accagctccg cggctacatc
2220gaggactccc aggacctgga aatctacctc atccgctaca acgccaagca
cgagatcgtg 2280aacgtgcctg gcaccggcag cctctggcct ctcagcgtgg
agaaccagat cggcccttgc 2340ggcgagccta accgctgcgc ccctcacctc
gagtggaacc ctgacctcca ctgctcgtgc 2400agggacggcg agaagtgcgc
ccaccatagc caccacttct ctctggacat cgacgtgggc 2460tgcaccgacc
tgaacgagga cctgggcgtg tgggttatct tcaagatcaa gacccaggac
2520ggtcacgcca ggctgggtaa cctggagttc cttgaggaaa agcctctgct
gggtgaggcc 2580ctggccaggg tcaagagggc tgagaagaaa tggagggata
agagggagac cctgcagctg 2640gagaccacta tcgtctacaa ggaggctaag
gagtctgtcg atgctctgtt cgtcaactct 2700cagtacgata gactgcaagc
tgataccaac atcgctatga tccacgctgc ggataagcgg 2760gtccaccgga
tccgggaggc ttaccttccg gagctttctg tcatcccggg tgtcaacgct
2820gcgatcttcg aggaacttga ggaacggatc ttcactgcgt ttagtcttta
cgatgcgcgg 2880aacatcatca agaacgggga cttcaacaat ggtctgctgt
gctggaacgt caagggtcat 2940gtcgaggtcg aggaacaaaa caatcatcgt
agtgtccttg tcattcctga gtgggaggcg 3000gaggtctctc aagaggtccg
tgtttgcccg gggcgtgggt acattcttcg tgttactgcg 3060tacaaggagg
ggtacgggga ggggtgcgtt actattcatg agattgagaa caatactgat
3120gagcttaagt tcaacaattg tgttgaggag gaggtttacc cgaacaatac
tgttacgtgc 3180atcaactaca cggcaacgca agaggaatac gaggggacgt
acacctcgcg taatagaggg 3240tatgatgagg cgtacggaaa caacccgtcg
gttccagcag attatgcctc ggtttatgag 3300gagaagtcgt acacggatag
acgacgcgag aatccatgtg agtcaaatcg aggatacgga 3360gattacacac
cattaccagc aggatacgtt acaaaggagt tggaatactt cccggaaaca
3420gataaagttt ggattgaaat cggagaaaca gaaggaacat tcatcgtcga
ctcagtagaa 3480ttgttgttga tggaagaatg a 3501301166PRTArtificial
sequenceAmino acid sequence of Cry1Da1 encoded by a synthetic DNA
sequence wherein an additional Alanine residue has been inserted at
position 2. 30Met Ala Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr
Asn Cys Leu1 5 10 15Ser Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg
Leu Glu Thr Gly 20 25 30Asn Thr Val Ala Asp Ile Ser Leu Gly Leu Ile
Asn Phe Leu Tyr Ser 35 40 45Asn Phe Val Pro Gly Gly Gly Phe Ile Val
Gly Leu Leu Glu Leu Ile 50 55 60Trp Gly Phe Ile Gly Pro Ser Gln Trp
Asp Ile Phe Leu Ala Gln Ile65 70 75 80Glu Gln Leu Ile Ser Gln Arg
Ile Glu Glu Phe Ala Arg Asn Gln Ala 85 90 95Ile Ser Arg Leu Glu Gly
Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg 100 105 110Ala Phe Ser Asp
Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu 115 120 125Glu Met
Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala 130 135
140Ile Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser
Val145 150 155 160Tyr Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu
Arg Asp Val Ser 165 170 175Val Phe Gly Glu Arg Trp Gly Tyr Asp Thr
Ala Thr Ile Asn Asn Arg 180 185 190Tyr Ser Asp Leu Thr Ser Leu Ile
His Val Tyr Thr Asn His Cys Val 195 200 205Asp Thr Tyr Asn Gln Gly
Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser 210 215 220Asp Trp Ile Val
Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val225 230 235 240Leu
Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro 245 250
255Ile Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro
260 265 270Phe Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr
Phe Ser 275 280 285Ala Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu
Val Asp Phe Leu 290 295 300Asn Ser Phe Thr Ile Tyr Thr Asp Ser Leu
Ala Arg Tyr Ala Tyr Trp305 310 315 320Gly Gly His Leu Val Asn Ser
Phe Arg Thr Gly Thr Thr Thr Asn Leu 325 330 335Ile Arg Ser Pro Leu
Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val 340 345 350Thr Ile Thr
Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr 355 360 365Ile
Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val 370 375
380Glu Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly
Pro385 390 395 400Ile Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala
Ser Val Ser Pro 405 410 415Ala Ile Gly Tyr Ser His Arg Leu Cys His
Ala Thr Phe Leu Glu Arg 420 425 430Ile Ser Gly Pro Arg Ile Ala Gly
Thr Val Phe Ser Trp Thr His Arg 435 440 445Ser Ala Ser Pro Thr Asn
Glu Val Ser Pro Ser Arg Ile Thr Gln Ile 450 455 460Pro Trp Val Lys
Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys465 470 475 480Gly
Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly 485 490
495Glu Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser
500 505 510Tyr Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser
Gly Thr 515 520 525Phe Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser
Phe Pro Lys Thr 530 535 540Met Asp Ala Gly Glu Pro Leu Thr Ser Arg
Ser Phe Ala His Thr Thr545 550 555 560Leu Phe Thr Pro Ile Thr Phe
Ser Arg Ala Gln Glu Glu Phe Asp Leu 565 570 575Tyr Ile Gln Ser Gly
Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val 580 585 590Thr Ala Thr
Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val 595 600 605Val
Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp 610 615
620Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys
Leu625 630 635 640Ser Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu
Ser Glu Lys Val 645 650 655Lys His Ala Lys Arg Leu Ser Asp Glu Arg
Asn Leu Leu Gln Asp Pro 660 665 670Asn Phe Arg Gly Ile Asn Arg Gln
Pro Asp Arg Gly Trp Arg Gly Ser 675 680 685Thr Asp Ile Thr Ile Gln
Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr 690 695 700Val Thr Leu Pro
Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr705 710 715 720Gln
Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu 725 730
735Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg
740 745 750Tyr Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly
Ser Leu 755 760 765Trp Pro Leu Ser Val Glu Asn Gln Ile Gly Pro Cys
Gly Glu Pro Asn 770 775 780Arg Cys Ala Pro His Leu Glu Trp Asn Pro
Asp Leu His Cys Ser Cys785 790 795 800Arg Asp Gly Glu Lys Cys Ala
His His Ser His His Phe Ser Leu Asp 805 810 815Ile Asp Val Gly Cys
Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val 820 825 830Ile Phe Lys
Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu 835 840 845Glu
Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val 850 855
860Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln
Leu865 870 875 880Glu Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu Ser
Val Asp Ala Leu 885 890 895Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln
Ala Asp Thr Asn Ile Ala 900 905 910Met Ile His Ala Ala Asp Lys Arg
Val His Arg Ile Arg Glu Ala Tyr 915 920 925Leu Pro Glu Leu Ser Val
Ile Pro Gly Val Asn Ala Ala Ile Phe Glu 930 935 940Glu Leu Glu Glu
Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg945 950 955 960Asn
Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn 965 970
975Val Lys Gly His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser Val
980 985 990Leu Val Ile Pro Glu Trp Glu Ala Glu Val Ser Gln Glu Val
Arg Val 995 1000 1005Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr
Ala Tyr Lys Glu 1010 1015 1020Gly Tyr Gly Glu Gly Cys Val Thr Ile
His Glu Ile Glu Asn Asn 1025 1030 1035Thr Asp Glu Leu Lys Phe Asn
Asn Cys Val Glu Glu Glu Val Tyr 1040 1045 1050Pro Asn Asn Thr Val
Thr Cys Ile Asn Tyr Thr Ala Thr Gln Glu 1055 1060 1065Glu Tyr Glu
Gly Thr Tyr Thr Ser Arg Asn Arg Gly Tyr Asp Glu 1070 1075 1080Ala
Tyr Gly Asn Asn Pro Ser Val Pro Ala Asp Tyr Ala Ser Val 1085 1090
1095Tyr Glu Glu Lys Ser Tyr Thr Asp Arg Arg Arg Glu Asn Pro Cys
1100 1105 1110Glu Ser Asn Arg Gly Tyr Gly Asp Tyr Thr Pro Leu Pro
Ala Gly 1115 1120 1125Tyr Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu
Thr Asp Lys Val 1130 1135 1140Trp Ile Glu Ile Gly Glu Thr Glu Gly
Thr Phe Ile Val Asp Ser 1145 1150 1155Val Glu Leu Leu Leu Met Glu
Glu 1160 1165313501DNAArtificial sequenceSynthetic DNA sequence
designed for plant expression encoding Cry1Da1_3 with an additional
Alanine residue inserted at position 2. 31atggctgaga tcaacaacca
gaaccagtgc gtcccgtaca actgcctgag caaccctaag 60gagatcatcc tgggtgagga
acgcctggag accggcaaca ccgtagccga cattagcctg 120ggcctcatca
acttcctcta cagcaacttc gtgcccggcg gtggcttcat cgtgggcctc
180ctggagctta tctggggctt catcggcccg tcccagtggg acatcttcct
cgcccagatc 240gagcaactga tcagccagcg gatcgaggag ttcgctagga
accaggccat ctcccgcctg 300gagggactct ccaacctcta caaggtgtac
gtgcgcgcgt tcagcgactg ggagaaggac 360ccgaccaacc cggccctccg
cgaggaaatg cgtatccagt tcaacgatat gaactcggcc 420ctcatcaccg
ccatcccgct cttccgcgtg cagaactacg aggtggccct cctgtccgtg
480tacgttcaag ccgccaacct ccacctctcc atcctccgcg acgtgagcgt
gttcggcgag 540cgctggggct acgacaccgc caccatcaac aaccgctact
ccgacctcac ctccctcatc 600cacgtttaca ccaaccactg cgtggacacg
tacaaccagg gcctccgccg cctggagggc 660cgcttcctct ccgactggat
cgtgtacaac cgcttccgcc gccagctcac catctccgtc 720ctggacatcg
tcgccttctt tcccaactac gacatccgca cctaccctat ccagaccgcc
780acccagctca cccgcgaggt ctacctcgac ctcccgttca tcaacgagaa
cctcagcccg 840gccgccagct acccgacctt ctccgccgct gagtccgcca
tcattcgcag cccgcacctc 900gtggacttcc tcaactcctt caccatctac
accgactccc tcgcccgcag cgcctactgg 960ggcggtcacc tcgtgaactc
cttccgcacc ggcaccacta ccaacctcat ccgcagcccg 1020ctctacggcc
gcgagggcaa caccgagcgc ccggtgacca tcaccgccag cccgagcgtg
1080cccatcttcc gcaccctcag ctacatcacc ggcctggaca acagcaaccc
tgtggcgggc 1140atcgagggcg tggagttcca gaacaccatc tccaggagca
tctaccgcaa gagcggccct 1200atcgacagct tcagcgagct gcctcctcag
gacgccagcg tgagccctgc catcggctac 1260agccacaggc tgtgccacgc
caccttcctg gagcgcatca gcggccctcg catcgcgggc 1320accgtgttct
cgtggaccca ccgcagcgcc tctcctacga acgaggtgtc tcctagtcgc
1380atcacccaga tcccttgggt caaggcccac accctggcta gtggcgctag
tgtcatcaag 1440ggccctggct tcaccggtgg tgacatcctg accaggaact
ctatgggcga gctgggcact 1500ctgagggtca ctttcactgg ccgcctgcct
cagtcttact acatccgctt ccgctacgct 1560agtgtcgcta accgctctgg
tactttccgc tactctcagc ctccgtctta cggtatctct 1620ttccctaaga
ctatggacgc tggtgagcct ctgaccagta ggagcttcgc tcacactact
1680ctgttcactc ctatcacttt ctctagggct caggaggagt tcgacctgta
catccagtct 1740ggtgtgtaca tcgacaggat cgagttcatc cccgtgaccg
ccacgttcga ggccgagtac 1800gaccttgagc gcgcccagaa ggtggtgaac
gccctcttca ctagcactaa ccagctaggc 1860ctgaagactg acgtgaccga
ctaccacatc gaccaagtga gcaacctagt ggcctgcctc 1920tccgacgagt
tctgcctcga cgagaagcgc gagctgtccg agaaggtgaa gcacgccaag
1980cgcctctccg acgagcgcaa cctgctccag gaccccaact tcaggggcat
caacaggcag 2040cccgaccgcg gctggcgcgg ctccaccgac atcaccatcc
agggcggtga cgacgtattc 2100aaggagaact acgttaccct ccccggcacc
ttcgacgagt gttaccccac ctacctctac 2160cagaagatcg acgagtccaa
gctgaaggcc tacacccgct accagctccg cggctacatc 2220gaggactccc
aggacctgga aatctacctc atccgctaca acgccaagca cgagatcgtg
2280aacgtgcctg gcaccggcag cctctggcct ctcagcgtgg agaaccagat
cggcccttgc 2340ggcgagccta accgctgcgc ccctcacctc gagtggaacc
ctgacctcca ctgctcgtgc 2400agggacggcg agaagtgcgc ccaccatagc
caccacttct ctctggacat cgacgtgggc 2460tgcaccgacc tgaacgagga
cctgggcgtg tgggttatct tcaagatcaa gacccaggac 2520ggtcacgcca
ggctgggtaa cctggagttc cttgaggaaa agcctctgct gggtgaggcc
2580ctggccaggg tcaagagggc tgagaagaaa tggagggata agagggagac
cctgcagctg 2640gagaccacta tcgtctacaa ggaggctaag gagtctgtcg
atgctctgtt cgtcaactct 2700cagtacgata gactgcaagc tgataccaac
atcgctatga tccacgctgc ggataagcgg 2760gtccaccgga tccgggaggc
ttaccttccg gagctttctg tcatcccggg tgtcaacgct 2820gcgatcttcg
aggaacttga ggaacggatc ttcactgcgt ttagtcttta cgatgcgcgg
2880aacatcatca agaacgggga cttcaacaat ggtctgctgt gctggaacgt
caagggtcat 2940gtcgaggtcg aggaacaaaa caatcatcgt agtgtccttg
tcattcctga gtgggaggcg 3000gaggtctctc aagaggtccg tgtttgcccg
gggcgtgggt acattcttcg tgttactgcg 3060tacaaggagg ggtacgggga
ggggtgcgtt actattcatg agattgagaa caatactgat 3120gagcttaagt
tcaacaattg tgttgaggag gaggtttacc cgaacaatac tgttacgtgc
3180atcaactaca cggcaacgca agaggaatac gaggggacgt acacctcgcg
taatagaggg 3240tatgatgagg cgtacggaaa caacccgtcg gttccagcag
attatgcctc ggtttatgag 3300gagaagtcgt acacggatag acgacgcgag
aatccatgtg agtcaaatcg aggatacgga 3360gattacacac cattaccagc
aggatacgtt acaaaggagt tggaatactt cccggaaaca 3420gataaagttt
ggattgaaat cggagaaaca gaaggaacat tcatcgtcga ctcagtagaa
3480ttgttgttga tggaagaatg a 3501321166PRTArtificial sequenceAmino
acid sequence of the engineered insecticidal protein Cry1Da1_3
encoded by a synthetic DNA sequence wherein an additional Alanine
residue has been inserted at position 2. 32Met Ala Glu Ile Asn Asn
Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu1 5 10 15Ser Asn Pro Lys Glu
Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly 20 25 30Asn Thr Val Ala
Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser 35 40 45Asn Phe Val
Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile 50 55 60Trp Gly
Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile65 70 75
80Glu Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala
85 90 95Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val
Arg 100 105 110Ala Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala
Leu Arg Glu 115 120 125Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser
Ala Leu Ile Thr Ala 130 135 140Ile Pro Leu Phe Arg Val Gln Asn Tyr
Glu Val Ala Leu Leu Ser Val145 150 155 160Tyr Val Gln Ala Ala Asn
Leu His Leu Ser Ile Leu Arg Asp Val Ser
165 170 175Val Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn
Asn Arg 180 185 190Tyr Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr
Asn His Cys Val 195 200 205Asp Thr Tyr Asn Gln Gly Leu Arg Arg Leu
Glu Gly Arg Phe Leu Ser 210 215 220Asp Trp Ile Val Tyr Asn Arg Phe
Arg Arg Gln Leu Thr Ile Ser Val225 230 235 240Leu Asp Ile Val Ala
Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro 245 250 255Ile Gln Thr
Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro 260 265 270Phe
Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser 275 280
285Ala Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu
290 295 300Asn Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala
Tyr Trp305 310 315 320Gly Gly His Leu Val Asn Ser Phe Arg Thr Gly
Thr Thr Thr Asn Leu 325 330 335Ile Arg Ser Pro Leu Tyr Gly Arg Glu
Gly Asn Thr Glu Arg Pro Val 340 345 350Thr Ile Thr Ala Ser Pro Ser
Val Pro Ile Phe Arg Thr Leu Ser Tyr 355 360 365Ile Thr Gly Leu Asp
Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val 370 375 380Glu Phe Gln
Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro385 390 395
400Ile Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro
405 410 415Ala Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu
Glu Arg 420 425 430Ile Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser
Trp Thr His Arg 435 440 445Ser Ala Ser Pro Thr Asn Glu Val Ser Pro
Ser Arg Ile Thr Gln Ile 450 455 460Pro Trp Val Lys Ala His Thr Leu
Ala Ser Gly Ala Ser Val Ile Lys465 470 475 480Gly Pro Gly Phe Thr
Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly 485 490 495Glu Leu Gly
Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser 500 505 510Tyr
Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr 515 520
525Phe Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr
530 535 540Met Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His
Thr Thr545 550 555 560Leu Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln
Glu Glu Phe Asp Leu 565 570 575Tyr Ile Gln Ser Gly Val Tyr Ile Asp
Arg Ile Glu Phe Ile Pro Val 580 585 590Thr Ala Thr Phe Glu Ala Glu
Tyr Asp Leu Glu Arg Ala Gln Lys Val 595 600 605Val Asn Ala Leu Phe
Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp 610 615 620Val Thr Asp
Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu625 630 635
640Ser Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val
645 650 655Lys His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln
Asp Pro 660 665 670Asn Phe Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly
Trp Arg Gly Ser 675 680 685Thr Asp Ile Thr Ile Gln Gly Gly Asp Asp
Val Phe Lys Glu Asn Tyr 690 695 700Val Thr Leu Pro Gly Thr Phe Asp
Glu Cys Tyr Pro Thr Tyr Leu Tyr705 710 715 720Gln Lys Ile Asp Glu
Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu 725 730 735Arg Gly Tyr
Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg 740 745 750Tyr
Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser Leu 755 760
765Trp Pro Leu Ser Val Glu Asn Gln Ile Gly Pro Cys Gly Glu Pro Asn
770 775 780Arg Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu His Cys
Ser Cys785 790 795 800Arg Asp Gly Glu Lys Cys Ala His His Ser His
His Phe Ser Leu Asp 805 810 815Ile Asp Val Gly Cys Thr Asp Leu Asn
Glu Asp Leu Gly Val Trp Val 820 825 830Ile Phe Lys Ile Lys Thr Gln
Asp Gly His Ala Arg Leu Gly Asn Leu 835 840 845Glu Phe Leu Glu Glu
Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val 850 855 860Lys Arg Ala
Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln Leu865 870 875
880Glu Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu
885 890 895Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn
Ile Ala 900 905 910Met Ile His Ala Ala Asp Lys Arg Val His Arg Ile
Arg Glu Ala Tyr 915 920 925Leu Pro Glu Leu Ser Val Ile Pro Gly Val
Asn Ala Ala Ile Phe Glu 930 935 940Glu Leu Glu Glu Arg Ile Phe Thr
Ala Phe Ser Leu Tyr Asp Ala Arg945 950 955 960Asn Ile Ile Lys Asn
Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn 965 970 975Val Lys Gly
His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser Val 980 985 990Leu
Val Ile Pro Glu Trp Glu Ala Glu Val Ser Gln Glu Val Arg Val 995
1000 1005Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr Ala Tyr Lys
Glu 1010 1015 1020Gly Tyr Gly Glu Gly Cys Val Thr Ile His Glu Ile
Glu Asn Asn 1025 1030 1035Thr Asp Glu Leu Lys Phe Asn Asn Cys Val
Glu Glu Glu Val Tyr 1040 1045 1050Pro Asn Asn Thr Val Thr Cys Ile
Asn Tyr Thr Ala Thr Gln Glu 1055 1060 1065Glu Tyr Glu Gly Thr Tyr
Thr Ser Arg Asn Arg Gly Tyr Asp Glu 1070 1075 1080Ala Tyr Gly Asn
Asn Pro Ser Val Pro Ala Asp Tyr Ala Ser Val 1085 1090 1095Tyr Glu
Glu Lys Ser Tyr Thr Asp Arg Arg Arg Glu Asn Pro Cys 1100 1105
1110Glu Ser Asn Arg Gly Tyr Gly Asp Tyr Thr Pro Leu Pro Ala Gly
1115 1120 1125Tyr Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu Thr Asp
Lys Val 1130 1135 1140Trp Ile Glu Ile Gly Glu Thr Glu Gly Thr Phe
Ile Val Asp Ser 1145 1150 1155Val Glu Leu Leu Leu Met Glu Glu 1160
1165333501DNAArtificial sequenceSynthetic DNA sequence designed for
plant expression encoding Cry1Da1_4 with an additional Alanine
residue inserted at position 2. 33atggctgaga tcaacaacca gaaccagtgc
gtcccgtaca actgcctgag caaccctaag 60gagatcatcc tgggtgagga acgcctggag
accggcaaca ccgtagccga cattagcctg 120ggcctcatca acttcctcta
cagcaacttc gtgcccggcg gtggcttcat cgtgggcctc 180ctggagctta
tctggggctt catcggcccg tcccagtggg acatcttcct cgcccagatc
240gagcaactga tcagccagcg gatcgaggag ttcgctagga accaggccat
ctcccgcctg 300gagggactct ccaacctcta caaggtgtac gtgcgcgcgt
tcagcgactg ggagaaggac 360ccgaccaacc cggccctccg cgaggaaatg
cgtatccagt tcaacgatat gaactcggcc 420ctcatcaccg ccatcccgct
cttccgcgtg cagaactacg aggtggccct cctgtccgtg 480tacgttcaag
ccgccaacct ccacctctcc atcctccgcg acgtgagcgt gttcggcgag
540cgctggggct acgacaccgc caccatcaac aaccgctact ccgacctcac
ctccctcatc 600cacgtttaca ccaaccactg cgtggacacg tacaaccagg
gcctccgccg cctggagggc 660cgcttcctct ccgactggat cgtgtacaac
cgcttccgcc gccagctcac catctccgtc 720ctggacatcg tcgccttctt
tcccaactac gacatccgca cctaccctat ccagaccgcc 780acccagctca
cccgcgaggt ctacctcgac ctcccgttca tcaacgagaa cctcagcccg
840gccgccagct acccgacctt ctccgccgct gagtccgcca tcattcgcag
cccgcacctc 900gtggacttcc tcaactcctt caccatctac accgactccc
tcgcccgcta cgcctactgg 960ggcggtcacc tcgtgaactc cttccgcacc
ggcaccacta ccaacctcat ccgcagcccg 1020ctctacggcc gcgagggcaa
caccgagcgc ccggtgacca tcaccgccag cccgagcgtg 1080cccatcttcc
gcaccctcag ctacatcacc ggcctggaca acaggaaccc tgtggcgggc
1140atcgagggcg tggagttcca gaacaccatc tccaggagca tctaccgcaa
gagcggccct 1200atcgacagct tcagcgagct gcctcctcag gacgccagcg
tgagccctgc catcggctac 1260agccacaggc tgtgccacgc caccttcctg
gagcgcatca gcggccctcg catcgcgggc 1320accgtgttct cgtggaccca
ccgcagcgcc tctcctacga acgaggtgtc tcctagtcgc 1380atcacccaga
tcccttgggt caaggcccac accctggcta gtggcgctag tgtcatcaag
1440ggccctggct tcaccggtgg tgacatcctg accaggaact ctatgggcga
gctgggcact 1500ctgagggtca ctttcactgg ccgcctgcct cagtcttact
acatccgctt ccgctacgct 1560agtgtcgcta accgctctgg tactttccgc
tactctcagc ctccgtctta cggtatctct 1620ttccctaaga ctatggacgc
tggtgagcct ctgaccagta ggagcttcgc tcacactact 1680ctgttcactc
ctatcacttt ctctagggct caggaggagt tcgacctgta catccagtct
1740ggtgtgtaca tcgacaggat cgagttcatc cccgtgaccg ccacgttcga
ggccgagtac 1800gaccttgagc gcgcccagaa ggtggtgaac gccctcttca
ctagcactaa ccagctaggc 1860ctgaagactg acgtgaccga ctaccacatc
gaccaagtga gcaacctagt ggcctgcctc 1920tccgacgagt tctgcctcga
cgagaagcgc gagctgtccg agaaggtgaa gcacgccaag 1980cgcctctccg
acgagcgcaa cctgctccag gaccccaact tcaggggcat caacaggcag
2040cccgaccgcg gctggcgcgg ctccaccgac atcaccatcc agggcggtga
cgacgtattc 2100aaggagaact acgttaccct ccccggcacc ttcgacgagt
gttaccccac ctacctctac 2160cagaagatcg acgagtccaa gctgaaggcc
tacacccgct accagctccg cggctacatc 2220gaggactccc aggacctgga
aatctacctc atccgctaca acgccaagca cgagatcgtg 2280aacgtgcctg
gcaccggcag cctctggcct ctcagcgtgg agaaccagat cggcccttgc
2340ggcgagccta accgctgcgc ccctcacctc gagtggaacc ctgacctcca
ctgctcgtgc 2400agggacggcg agaagtgcgc ccaccatagc caccacttct
ctctggacat cgacgtgggc 2460tgcaccgacc tgaacgagga cctgggcgtg
tgggttatct tcaagatcaa gacccaggac 2520ggtcacgcca ggctgggtaa
cctggagttc cttgaggaaa agcctctgct gggtgaggcc 2580ctggccaggg
tcaagagggc tgagaagaaa tggagggata agagggagac cctgcagctg
2640gagaccacta tcgtctacaa ggaggctaag gagtctgtcg atgctctgtt
cgtcaactct 2700cagtacgata gactgcaagc tgataccaac atcgctatga
tccacgctgc ggataagcgg 2760gtccaccgga tccgggaggc ttaccttccg
gagctttctg tcatcccggg tgtcaacgct 2820gcgatcttcg aggaacttga
ggaacggatc ttcactgcgt ttagtcttta cgatgcgcgg 2880aacatcatca
agaacgggga cttcaacaat ggtctgctgt gctggaacgt caagggtcat
2940gtcgaggtcg aggaacaaaa caatcatcgt agtgtccttg tcattcctga
gtgggaggcg 3000gaggtctctc aagaggtccg tgtttgcccg gggcgtgggt
acattcttcg tgttactgcg 3060tacaaggagg ggtacgggga ggggtgcgtt
actattcatg agattgagaa caatactgat 3120gagcttaagt tcaacaattg
tgttgaggag gaggtttacc cgaacaatac tgttacgtgc 3180atcaactaca
cggcaacgca agaggaatac gaggggacgt acacctcgcg taatagaggg
3240tatgatgagg cgtacggaaa caacccgtcg gttccagcag attatgcctc
ggtttatgag 3300gagaagtcgt acacggatag acgacgcgag aatccatgtg
agtcaaatcg aggatacgga 3360gattacacac cattaccagc aggatacgtt
acaaaggagt tggaatactt cccggaaaca 3420gataaagttt ggattgaaat
cggagaaaca gaaggaacat tcatcgtcga ctcagtagaa 3480ttgttgttga
tggaagaatg a 3501341166PRTArtificial sequenceAmino acid sequence of
the engineered insecticidal protein Cry1Da1_4 encoded by a
synthetic DNA sequence wherein an additional Alanine residue has
been inserted at position 2. 34Met Ala Glu Ile Asn Asn Gln Asn Gln
Cys Val Pro Tyr Asn Cys Leu1 5 10 15Ser Asn Pro Lys Glu Ile Ile Leu
Gly Glu Glu Arg Leu Glu Thr Gly 20 25 30Asn Thr Val Ala Asp Ile Ser
Leu Gly Leu Ile Asn Phe Leu Tyr Ser 35 40 45Asn Phe Val Pro Gly Gly
Gly Phe Ile Val Gly Leu Leu Glu Leu Ile 50 55 60Trp Gly Phe Ile Gly
Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile65 70 75 80Glu Gln Leu
Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala 85 90 95Ile Ser
Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg 100 105
110Ala Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu
115 120 125Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile
Thr Ala 130 135 140Ile Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala
Leu Leu Ser Val145 150 155 160Tyr Val Gln Ala Ala Asn Leu His Leu
Ser Ile Leu Arg Asp Val Ser 165 170 175Val Phe Gly Glu Arg Trp Gly
Tyr Asp Thr Ala Thr Ile Asn Asn Arg 180 185 190Tyr Ser Asp Leu Thr
Ser Leu Ile His Val Tyr Thr Asn His Cys Val 195 200 205Asp Thr Tyr
Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser 210 215 220Asp
Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val225 230
235 240Leu Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr
Pro 245 250 255Ile Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu
Asp Leu Pro 260 265 270Phe Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser
Tyr Pro Thr Phe Ser 275 280 285Ala Ala Glu Ser Ala Ile Ile Arg Ser
Pro His Leu Val Asp Phe Leu 290 295 300Asn Ser Phe Thr Ile Tyr Thr
Asp Ser Leu Ala Arg Tyr Ala Tyr Trp305 310 315 320Gly Gly His Leu
Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu 325 330 335Ile Arg
Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val 340 345
350Thr Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr
355 360 365Ile Thr Gly Leu Asp Asn Arg Asn Pro Val Ala Gly Ile Glu
Gly Val 370 375 380Glu Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg
Lys Ser Gly Pro385 390 395 400Ile Asp Ser Phe Ser Glu Leu Pro Pro
Gln Asp Ala Ser Val Ser Pro 405 410 415Ala Ile Gly Tyr Ser His Arg
Leu Cys His Ala Thr Phe Leu Glu Arg 420 425 430Ile Ser Gly Pro Arg
Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg 435 440 445Ser Ala Ser
Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile 450 455 460Pro
Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys465 470
475 480Gly Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met
Gly 485 490 495Glu Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu
Pro Gln Ser 500 505 510Tyr Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala
Asn Arg Ser Gly Thr 515 520 525Phe Arg Tyr Ser Gln Pro Pro Ser Tyr
Gly Ile Ser Phe Pro Lys Thr 530 535 540Met Asp Ala Gly Glu Pro Leu
Thr Ser Arg Ser Phe Ala His Thr Thr545 550 555 560Leu Phe Thr Pro
Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu 565 570 575Tyr Ile
Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val 580 585
590Thr Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val
595 600 605Val Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys
Thr Asp 610 615 620Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu
Val Ala Cys Leu625 630 635 640Ser Asp Glu Phe Cys Leu Asp Glu Lys
Arg Glu Leu Ser Glu Lys Val 645 650 655Lys His Ala Lys Arg Leu Ser
Asp Glu Arg Asn Leu Leu Gln Asp Pro 660 665 670Asn Phe Arg Gly Ile
Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser 675 680 685Thr Asp Ile
Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr 690 695 700Val
Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr705 710
715 720Gln Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln
Leu 725 730 735Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr
Leu Ile Arg 740 745 750Tyr Asn Ala Lys His Glu Ile Val Asn Val Pro
Gly Thr Gly Ser Leu 755 760 765Trp Pro Leu Ser Val Glu Asn Gln Ile
Gly Pro Cys Gly Glu Pro Asn 770 775 780Arg Cys Ala Pro His Leu Glu
Trp Asn Pro Asp Leu His Cys Ser Cys785 790 795 800Arg Asp Gly Glu
Lys Cys Ala His His Ser His His Phe Ser Leu Asp 805 810 815Ile Asp
Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val 820 825
830Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala
Arg Leu Gly Asn Leu 835 840 845Glu Phe Leu Glu Glu Lys Pro Leu Leu
Gly Glu Ala Leu Ala Arg Val 850 855 860Lys Arg Ala Glu Lys Lys Trp
Arg Asp Lys Arg Glu Thr Leu Gln Leu865 870 875 880Glu Thr Thr Ile
Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu 885 890 895Phe Val
Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala 900 905
910Met Ile His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr
915 920 925Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile
Phe Glu 930 935 940Glu Leu Glu Glu Arg Ile Phe Thr Ala Phe Ser Leu
Tyr Asp Ala Arg945 950 955 960Asn Ile Ile Lys Asn Gly Asp Phe Asn
Asn Gly Leu Leu Cys Trp Asn 965 970 975Val Lys Gly His Val Glu Val
Glu Glu Gln Asn Asn His Arg Ser Val 980 985 990Leu Val Ile Pro Glu
Trp Glu Ala Glu Val Ser Gln Glu Val Arg Val 995 1000 1005Cys Pro
Gly Arg Gly Tyr Ile Leu Arg Val Thr Ala Tyr Lys Glu 1010 1015
1020Gly Tyr Gly Glu Gly Cys Val Thr Ile His Glu Ile Glu Asn Asn
1025 1030 1035Thr Asp Glu Leu Lys Phe Asn Asn Cys Val Glu Glu Glu
Val Tyr 1040 1045 1050Pro Asn Asn Thr Val Thr Cys Ile Asn Tyr Thr
Ala Thr Gln Glu 1055 1060 1065Glu Tyr Glu Gly Thr Tyr Thr Ser Arg
Asn Arg Gly Tyr Asp Glu 1070 1075 1080Ala Tyr Gly Asn Asn Pro Ser
Val Pro Ala Asp Tyr Ala Ser Val 1085 1090 1095Tyr Glu Glu Lys Ser
Tyr Thr Asp Arg Arg Arg Glu Asn Pro Cys 1100 1105 1110Glu Ser Asn
Arg Gly Tyr Gly Asp Tyr Thr Pro Leu Pro Ala Gly 1115 1120 1125Tyr
Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu Thr Asp Lys Val 1130 1135
1140Trp Ile Glu Ile Gly Glu Thr Glu Gly Thr Phe Ile Val Asp Ser
1145 1150 1155Val Glu Leu Leu Leu Met Glu Glu 1160
1165353501DNAArtificial sequenceSynthetic DNA sequence designed for
plant expression encoding Cry1Da1_5 with an additional Alanine
residue inserted at position 2. 35atggctgaga tcaacaacca gaaccagtgc
gtcccgtaca actgcctgag caaccctaag 60gagatcatcc tgggtgagga acgcctggag
accggcaaca ccgtagccga cattagcctg 120ggcctcatca acttcctcta
cagcaacttc gtgcccggcg gtggcttcat cgtgggcctc 180ctggagctta
tctggggctt catcggcccg tcccagtggg acatcttcct cgcccagatc
240gagcaactga tcagccagcg gatcgaggag ttcgctagga accaggccat
ctcccgcctg 300gagggactct ccaacctcta caaggtgtac gtgcgcgcgt
tcagcgactg ggagaaggac 360ccgaccaacc cggccctccg cgaggaaatg
cgtatccagt tcaacgatat gaactcggcc 420ctcatcaccg ccatcccgct
cttccgcgtg cagaactacg aggtggccct cctgtccgtg 480tacgttcaag
ccgccaacct ccacctctcc atcctccgcg acgtgagcgt gttcggcgag
540cgctggggct acgacaccgc caccatcaac aaccgctact ccgacctcac
ctccctcatc 600cacgtttaca ccaaccactg cgtggacacg tacaaccagg
gcctccgccg cctggagggc 660cgcttcctct ccgactggat cgtgtacaac
cgcttccgcc gccagctcac catctccgtc 720ctggacatcg tcgccttctt
tcccaactac gacatccgca cctaccctat ccagaccgcc 780acccagctca
cccgcgaggt ctacctcgac ctcccgttca tcaacgagaa cctcagcccg
840gccgccagct acccgacctt ctccgccgct gagtccgcca tcattcgcag
cccgcacctc 900gtggacttcc tcaactcctt caccatctac accgactccc
tcgcccgcag cgcctactgg 960ggcggtcacc tcgtgaactc cttccgcacc
ggcaccacta ccaacctcat ccgcagcccg 1020ctctacggcc gcgagggcaa
caccgagcgc ccggtgacca tcaccgccag cccgagcgtg 1080cccatcttcc
gcaccctcag ctaccgcacc ggcctggaca acagcaaccc tgtggcgggc
1140atcgagggcg tggagttcca gaacaccatc tccaggagca tctaccgcaa
gagcggccct 1200atcgacagct tcagcgagct gcctcctcag gacgccagcg
tgagccctgc catcggctac 1260agccacaggc tgtgccacgc caccttcctg
gagcgcatca gcggccctcg catcgcgggc 1320accgtgttct cgtggaccca
ccgcagcgcc tctcctacga acgaggtgtc tcctagtcgc 1380atcacccaga
tcccttgggt caaggcccac accctggcta gtggcgctag tgtcatcaag
1440ggccctggct tcaccggtgg tgacatcctg accaggaact ctatgggcga
gctgggcact 1500ctgagggtca ctttcactgg ccgcctgcct cagtcttact
acatccgctt ccgctacgct 1560agtgtcgcta accgctctgg tactttccgc
tactctcagc ctccgtctta cggtatctct 1620ttccctaaga ctatggacgc
tggtgagcct ctgaccagta ggagcttcgc tcacactact 1680ctgttcactc
ctatcacttt ctctagggct caggaggagt tcgacctgta catccagtct
1740ggtgtgtaca tcgacaggat cgagttcatc cccgtgaccg ccacgttcga
ggccgagtac 1800gaccttgagc gcgcccagaa ggtggtgaac gccctcttca
ctagcactaa ccagctaggc 1860ctgaagactg acgtgaccga ctaccacatc
gaccaagtga gcaacctagt ggcctgcctc 1920tccgacgagt tctgcctcga
cgagaagcgc gagctgtccg agaaggtgaa gcacgccaag 1980cgcctctccg
acgagcgcaa cctgctccag gaccccaact tcaggggcat caacaggcag
2040cccgaccgcg gctggcgcgg ctccaccgac atcaccatcc agggcggtga
cgacgtattc 2100aaggagaact acgttaccct ccccggcacc ttcgacgagt
gttaccccac ctacctctac 2160cagaagatcg acgagtccaa gctgaaggcc
tacacccgct accagctccg cggctacatc 2220gaggactccc aggacctgga
aatctacctc atccgctaca acgccaagca cgagatcgtg 2280aacgtgcctg
gcaccggcag cctctggcct ctcagcgtgg agaaccagat cggcccttgc
2340ggcgagccta accgctgcgc ccctcacctc gagtggaacc ctgacctcca
ctgctcgtgc 2400agggacggcg agaagtgcgc ccaccatagc caccacttct
ctctggacat cgacgtgggc 2460tgcaccgacc tgaacgagga cctgggcgtg
tgggttatct tcaagatcaa gacccaggac 2520ggtcacgcca ggctgggtaa
cctggagttc cttgaggaaa agcctctgct gggtgaggcc 2580ctggccaggg
tcaagagggc tgagaagaaa tggagggata agagggagac cctgcagctg
2640gagaccacta tcgtctacaa ggaggctaag gagtctgtcg atgctctgtt
cgtcaactct 2700cagtacgata gactgcaagc tgataccaac atcgctatga
tccacgctgc ggataagcgg 2760gtccaccgga tccgggaggc ttaccttccg
gagctttctg tcatcccggg tgtcaacgct 2820gcgatcttcg aggaacttga
ggaacggatc ttcactgcgt ttagtcttta cgatgcgcgg 2880aacatcatca
agaacgggga cttcaacaat ggtctgctgt gctggaacgt caagggtcat
2940gtcgaggtcg aggaacaaaa caatcatcgt agtgtccttg tcattcctga
gtgggaggcg 3000gaggtctctc aagaggtccg tgtttgcccg gggcgtgggt
acattcttcg tgttactgcg 3060tacaaggagg ggtacgggga ggggtgcgtt
actattcatg agattgagaa caatactgat 3120gagcttaagt tcaacaattg
tgttgaggag gaggtttacc cgaacaatac tgttacgtgc 3180atcaactaca
cggcaacgca agaggaatac gaggggacgt acacctcgcg taatagaggg
3240tatgatgagg cgtacggaaa caacccgtcg gttccagcag attatgcctc
ggtttatgag 3300gagaagtcgt acacggatag acgacgcgag aatccatgtg
agtcaaatcg aggatacgga 3360gattacacac cattaccagc aggatacgtt
acaaaggagt tggaatactt cccggaaaca 3420gataaagttt ggattgaaat
cggagaaaca gaaggaacat tcatcgtcga ctcagtagaa 3480ttgttgttga
tggaagaatg a 3501361166PRTArtificial sequenceAmino acid sequence of
the engineered insecticidal protein Cry1Da1_5 encoded by a
synthetic DNA sequence wherein an additional Alanine residue has
been inserted at position 2. 36Met Ala Glu Ile Asn Asn Gln Asn Gln
Cys Val Pro Tyr Asn Cys Leu1 5 10 15Ser Asn Pro Lys Glu Ile Ile Leu
Gly Glu Glu Arg Leu Glu Thr Gly 20 25 30Asn Thr Val Ala Asp Ile Ser
Leu Gly Leu Ile Asn Phe Leu Tyr Ser 35 40 45Asn Phe Val Pro Gly Gly
Gly Phe Ile Val Gly Leu Leu Glu Leu Ile 50 55 60Trp Gly Phe Ile Gly
Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile65 70 75 80Glu Gln Leu
Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala 85 90 95Ile Ser
Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg 100 105
110Ala Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu
115 120 125Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile
Thr Ala 130 135 140Ile Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala
Leu Leu Ser Val145 150 155 160Tyr Val Gln Ala Ala Asn Leu His Leu
Ser Ile Leu Arg Asp Val Ser 165 170 175Val Phe Gly Glu Arg Trp Gly
Tyr Asp Thr Ala Thr Ile Asn Asn Arg 180 185 190Tyr Ser Asp Leu Thr
Ser Leu Ile His Val Tyr Thr Asn His Cys Val 195 200 205Asp Thr Tyr
Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser 210 215 220Asp
Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val225 230
235 240Leu Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr
Pro 245 250 255Ile Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu
Asp Leu Pro 260 265 270Phe Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser
Tyr Pro Thr Phe Ser 275 280 285Ala Ala Glu Ser Ala Ile Ile Arg Ser
Pro His Leu Val Asp Phe Leu 290 295 300Asn Ser Phe Thr Ile Tyr Thr
Asp Ser Leu Ala Arg Ser Ala Tyr Trp305 310 315 320Gly Gly His Leu
Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu 325 330 335Ile Arg
Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val 340 345
350Thr Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr
355 360 365Arg Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu
Gly Val 370 375 380Glu Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg
Lys Ser Gly Pro385 390 395 400Ile Asp Ser Phe Ser Glu Leu Pro Pro
Gln Asp Ala Ser Val Ser Pro 405 410 415Ala Ile Gly Tyr Ser His Arg
Leu Cys His Ala Thr Phe Leu Glu Arg 420 425 430Ile Ser Gly Pro Arg
Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg 435 440 445Ser Ala Ser
Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile 450 455 460Pro
Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys465 470
475 480Gly Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met
Gly 485 490 495Glu Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu
Pro Gln Ser 500 505 510Tyr Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala
Asn Arg Ser Gly Thr 515 520 525Phe Arg Tyr Ser Gln Pro Pro Ser Tyr
Gly Ile Ser Phe Pro Lys Thr 530 535 540Met Asp Ala Gly Glu Pro Leu
Thr Ser Arg Ser Phe Ala His Thr Thr545 550 555 560Leu Phe Thr Pro
Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu 565 570 575Tyr Ile
Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val 580 585
590Thr Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val
595 600 605Val Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys
Thr Asp 610 615 620Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu
Val Ala Cys Leu625 630 635 640Ser Asp Glu Phe Cys Leu Asp Glu Lys
Arg Glu Leu Ser Glu Lys Val 645 650 655Lys His Ala Lys Arg Leu Ser
Asp Glu Arg Asn Leu Leu Gln Asp Pro 660 665 670Asn Phe Arg Gly Ile
Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser 675 680 685Thr Asp Ile
Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr 690 695 700Val
Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr705 710
715 720Gln Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln
Leu 725 730 735Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr
Leu Ile Arg 740 745 750Tyr Asn Ala Lys His Glu Ile Val Asn Val Pro
Gly Thr Gly Ser Leu 755 760 765Trp Pro Leu Ser Val Glu Asn Gln Ile
Gly Pro Cys Gly Glu Pro Asn 770 775 780Arg Cys Ala Pro His Leu Glu
Trp Asn Pro Asp Leu His Cys Ser Cys785 790 795 800Arg Asp Gly Glu
Lys Cys Ala His His Ser His His Phe Ser Leu Asp 805 810 815Ile Asp
Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val 820 825
830Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu
835 840 845Glu Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala
Arg Val 850 855 860Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu
Thr Leu Gln Leu865 870 875 880Glu Thr Thr Ile Val Tyr Lys Glu Ala
Lys Glu Ser Val Asp Ala Leu 885 890 895Phe Val Asn Ser Gln Tyr Asp
Arg Leu Gln Ala Asp Thr Asn Ile Ala 900 905 910Met Ile His Ala Ala
Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr 915 920 925Leu Pro Glu
Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu 930 935 940Glu
Leu Glu Glu Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg945 950
955 960Asn Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp
Asn 965 970 975Val Lys Gly His Val Glu Val Glu Glu Gln Asn Asn His
Arg Ser Val 980 985 990Leu Val Ile Pro Glu Trp Glu Ala Glu Val Ser
Gln Glu Val Arg Val 995 1000 1005Cys Pro Gly Arg Gly Tyr Ile Leu
Arg Val Thr Ala Tyr Lys Glu 1010 1015 1020Gly Tyr Gly Glu Gly Cys
Val Thr Ile His Glu Ile Glu Asn Asn 1025 1030 1035Thr Asp Glu Leu
Lys Phe Asn Asn Cys Val Glu Glu Glu Val Tyr 1040 1045 1050Pro Asn
Asn Thr Val Thr Cys Ile Asn Tyr Thr Ala Thr Gln Glu 1055 1060
1065Glu Tyr Glu Gly Thr Tyr Thr Ser Arg Asn Arg Gly Tyr Asp Glu
1070 1075 1080Ala Tyr Gly Asn Asn Pro Ser Val Pro Ala Asp Tyr Ala
Ser Val 1085 1090 1095Tyr Glu Glu Lys Ser Tyr Thr Asp Arg Arg Arg
Glu Asn Pro Cys 1100 1105 1110Glu Ser Asn Arg Gly Tyr Gly Asp Tyr
Thr Pro Leu Pro Ala Gly 1115 1120 1125Tyr Val Thr Lys Glu Leu Glu
Tyr Phe Pro Glu Thr Asp Lys Val 1130 1135 1140Trp Ile Glu Ile Gly
Glu Thr Glu Gly Thr Phe Ile Val Asp Ser 1145 1150 1155Val Glu Leu
Leu Leu Met Glu Glu 1160 1165373501DNAArtificial sequenceSynthetic
DNA sequence designed for plant expression encoding Cry1Da1_6 with
an additional Alanine residue inserted at position 2. 37atggctgaga
tcaacaacca gaaccagtgc gtcccgtaca actgcctgag caaccctaag 60gagatcatcc
tgggtgagga acgcctggag accggcaaca ccgtagccga cattagcctg
120ggcctcatca acttcctcta cagcaacttc gtgcccggcg gtggcttcat
cgtgggcctc 180ctggagctta tctggggctt catcggcccg tcccagtggg
acatcttcct cgcccagatc 240gagcaactga tcagccagcg gatcgaggag
ttcgctagga accaggccat ctcccgcctg 300gagggactct ccaacctcta
caaggtgtac gtgcgcgcgt tcagcgactg ggagaaggac 360ccgaccaacc
cggccctccg cgaggaaatg cgtatccagt tcaacgatat gaactcggcc
420ctcatcaccg ccatcccgct cttccgcgtg cagaactacg aggtggccct
cctgtccgtg 480tacgttcaag ccgccaacct ccacctctcc atcctccgcg
acgtgagcgt gttcggcgag 540cgctggggct acgacaccgc caccatcaac
aaccgctact ccgacctcac ctccctcatc 600cacgtttaca ccaaccactg
cgtggacacg tacaaccagg gcctccgccg cctggagggc 660cgcttcctct
ccgactggat cgtgtacaac cgcttccgcc gccagctcac catctccgtc
720ctggacatcg tcgccttctt tcccaactac gacatccgca cctaccctat
ccagaccgcc 780acccagctca cccgcgaggt ctacctcgac ctcccgttca
tcaacgagaa cctcagcccg 840gccgccaagt acccgacctt ctccgccgct
gagtccgcca tcattcgcag cccgcacctc 900gtggacttcc tcaactcctt
caccatctac accgactccc tcgcccgcag cgcctactgg 960ggcggtcacc
tcgtgaactc cttccgcacc ggcaccacta ccaacctcat ccgcagcccg
1020ctctacggcc gcgagggcaa caccgagcgc ccggtgacca tcaccgccag
cccgagcgtg 1080cccatcttcc gcaccctcag ctaccccacc ggcctggaca
acagcaaccc tgtggcgggc 1140atcgagggcg tggagttcca gaacaccatc
tccaggagca tctaccgcaa gagcggccct 1200atcgacagct tcagcgagct
gcctcctcag gacgccagcg tgagccctgc catcggctac 1260agccacaggc
tgtgccacgc caccttcctg gagcgcatca gcggccctcg catcgcgggc
1320accgtgttct cgtggaccca ccgcagcgcc tctcctacga acgaggtgtc
tcctagtcgc 1380atcacccaga tcccttgggt caaggcccac accctggcta
gtggcgctag tgtcatcaag 1440ggccctggct tcaccggtgg tgacatcctg
accaggaact ctatgggcga gctgggcact 1500ctgagggtca ctttcactgg
ccgcctgcct cagtcttact acatccgctt ccgctacgct 1560agtgtcgcta
accgctctgg tactttccgc tactctcagc ctccgtctta cggtatctct
1620ttccctaaga ctatggacgc tggtgagcct ctgaccagta ggagcttcgc
tcacactact 1680ctgttcactc ctatcacttt ctctagggct caggaggagt
tcgacctgta catccagtct 1740ggtgtgtaca tcgacaggat cgagttcatc
cccgtgaccg ccacgttcga ggccgagtac 1800gaccttgagc gcgcccagaa
ggtggtgaac gccctcttca ctagcactaa ccagctaggc 1860ctgaagactg
acgtgaccga ctaccacatc gaccaagtga
gcaacctagt ggcctgcctc 1920tccgacgagt tctgcctcga cgagaagcgc
gagctgtccg agaaggtgaa gcacgccaag 1980cgcctctccg acgagcgcaa
cctgctccag gaccccaact tcaggggcat caacaggcag 2040cccgaccgcg
gctggcgcgg ctccaccgac atcaccatcc agggcggtga cgacgtattc
2100aaggagaact acgttaccct ccccggcacc ttcgacgagt gttaccccac
ctacctctac 2160cagaagatcg acgagtccaa gctgaaggcc tacacccgct
accagctccg cggctacatc 2220gaggactccc aggacctgga aatctacctc
atccgctaca acgccaagca cgagatcgtg 2280aacgtgcctg gcaccggcag
cctctggcct ctcagcgtgg agaaccagat cggcccttgc 2340ggcgagccta
accgctgcgc ccctcacctc gagtggaacc ctgacctcca ctgctcgtgc
2400agggacggcg agaagtgcgc ccaccatagc caccacttct ctctggacat
cgacgtgggc 2460tgcaccgacc tgaacgagga cctgggcgtg tgggttatct
tcaagatcaa gacccaggac 2520ggtcacgcca ggctgggtaa cctggagttc
cttgaggaaa agcctctgct gggtgaggcc 2580ctggccaggg tcaagagggc
tgagaagaaa tggagggata agagggagac cctgcagctg 2640gagaccacta
tcgtctacaa ggaggctaag gagtctgtcg atgctctgtt cgtcaactct
2700cagtacgata gactgcaagc tgataccaac atcgctatga tccacgctgc
ggataagcgg 2760gtccaccgga tccgggaggc ttaccttccg gagctttctg
tcatcccggg tgtcaacgct 2820gcgatcttcg aggaacttga ggaacggatc
ttcactgcgt ttagtcttta cgatgcgcgg 2880aacatcatca agaacgggga
cttcaacaat ggtctgctgt gctggaacgt caagggtcat 2940gtcgaggtcg
aggaacaaaa caatcatcgt agtgtccttg tcattcctga gtgggaggcg
3000gaggtctctc aagaggtccg tgtttgcccg gggcgtgggt acattcttcg
tgttactgcg 3060tacaaggagg ggtacgggga ggggtgcgtt actattcatg
agattgagaa caatactgat 3120gagcttaagt tcaacaattg tgttgaggag
gaggtttacc cgaacaatac tgttacgtgc 3180atcaactaca cggcaacgca
agaggaatac gaggggacgt acacctcgcg taatagaggg 3240tatgatgagg
cgtacggaaa caacccgtcg gttccagcag attatgcctc ggtttatgag
3300gagaagtcgt acacggatag acgacgcgag aatccatgtg agtcaaatcg
aggatacgga 3360gattacacac cattaccagc aggatacgtt acaaaggagt
tggaatactt cccggaaaca 3420gataaagttt ggattgaaat cggagaaaca
gaaggaacat tcatcgtcga ctcagtagaa 3480ttgttgttga tggaagaatg a
3501381166PRTArtificial sequenceAmino acid sequence of the
engineered insecticidal protein Cry1Da1_6 encoded by a synthetic
DNA sequence wherein an additional Alanine residue has been
inserted at position 2. 38Met Ala Glu Ile Asn Asn Gln Asn Gln Cys
Val Pro Tyr Asn Cys Leu1 5 10 15Ser Asn Pro Lys Glu Ile Ile Leu Gly
Glu Glu Arg Leu Glu Thr Gly 20 25 30Asn Thr Val Ala Asp Ile Ser Leu
Gly Leu Ile Asn Phe Leu Tyr Ser 35 40 45Asn Phe Val Pro Gly Gly Gly
Phe Ile Val Gly Leu Leu Glu Leu Ile 50 55 60Trp Gly Phe Ile Gly Pro
Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile65 70 75 80Glu Gln Leu Ile
Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala 85 90 95Ile Ser Arg
Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg 100 105 110Ala
Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu 115 120
125Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala
130 135 140Ile Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu
Ser Val145 150 155 160Tyr Val Gln Ala Ala Asn Leu His Leu Ser Ile
Leu Arg Asp Val Ser 165 170 175Val Phe Gly Glu Arg Trp Gly Tyr Asp
Thr Ala Thr Ile Asn Asn Arg 180 185 190Tyr Ser Asp Leu Thr Ser Leu
Ile His Val Tyr Thr Asn His Cys Val 195 200 205Asp Thr Tyr Asn Gln
Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser 210 215 220Asp Trp Ile
Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val225 230 235
240Leu Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro
245 250 255Ile Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp
Leu Pro 260 265 270Phe Ile Asn Glu Asn Leu Ser Pro Ala Ala Lys Tyr
Pro Thr Phe Ser 275 280 285Ala Ala Glu Ser Ala Ile Ile Arg Ser Pro
His Leu Val Asp Phe Leu 290 295 300Asn Ser Phe Thr Ile Tyr Thr Asp
Ser Leu Ala Arg Ser Ala Tyr Trp305 310 315 320Gly Gly His Leu Val
Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu 325 330 335Ile Arg Ser
Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val 340 345 350Thr
Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr 355 360
365Pro Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val
370 375 380Glu Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser
Gly Pro385 390 395 400Ile Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp
Ala Ser Val Ser Pro 405 410 415Ala Ile Gly Tyr Ser His Arg Leu Cys
His Ala Thr Phe Leu Glu Arg 420 425 430Ile Ser Gly Pro Arg Ile Ala
Gly Thr Val Phe Ser Trp Thr His Arg 435 440 445Ser Ala Ser Pro Thr
Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile 450 455 460Pro Trp Val
Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys465 470 475
480Gly Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly
485 490 495Glu Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro
Gln Ser 500 505 510Tyr Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn
Arg Ser Gly Thr 515 520 525Phe Arg Tyr Ser Gln Pro Pro Ser Tyr Gly
Ile Ser Phe Pro Lys Thr 530 535 540Met Asp Ala Gly Glu Pro Leu Thr
Ser Arg Ser Phe Ala His Thr Thr545 550 555 560Leu Phe Thr Pro Ile
Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu 565 570 575Tyr Ile Gln
Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val 580 585 590Thr
Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val 595 600
605Val Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp
610 615 620Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala
Cys Leu625 630 635 640Ser Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu
Leu Ser Glu Lys Val 645 650 655Lys His Ala Lys Arg Leu Ser Asp Glu
Arg Asn Leu Leu Gln Asp Pro 660 665 670Asn Phe Arg Gly Ile Asn Arg
Gln Pro Asp Arg Gly Trp Arg Gly Ser 675 680 685Thr Asp Ile Thr Ile
Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr 690 695 700Val Thr Leu
Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr705 710 715
720Gln Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu
725 730 735Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu
Ile Arg 740 745 750Tyr Asn Ala Lys His Glu Ile Val Asn Val Pro Gly
Thr Gly Ser Leu 755 760 765Trp Pro Leu Ser Val Glu Asn Gln Ile Gly
Pro Cys Gly Glu Pro Asn 770 775 780Arg Cys Ala Pro His Leu Glu Trp
Asn Pro Asp Leu His Cys Ser Cys785 790 795 800Arg Asp Gly Glu Lys
Cys Ala His His Ser His His Phe Ser Leu Asp 805 810 815Ile Asp Val
Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val 820 825 830Ile
Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu 835 840
845Glu Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val
850 855 860Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu
Gln Leu865 870 875 880Glu Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu
Ser Val Asp Ala Leu 885 890 895Phe Val Asn Ser Gln Tyr Asp Arg Leu
Gln Ala Asp Thr Asn Ile Ala 900 905 910Met Ile His Ala Ala Asp Lys
Arg Val His Arg Ile Arg Glu Ala Tyr 915 920 925Leu Pro Glu Leu Ser
Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu 930 935 940Glu Leu Glu
Glu Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg945 950 955
960Asn Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn
965 970 975Val Lys Gly His Val Glu Val Glu Glu Gln Asn Asn His Arg
Ser Val 980 985 990Leu Val Ile Pro Glu Trp Glu Ala Glu Val Ser Gln
Glu Val Arg Val 995 1000 1005Cys Pro Gly Arg Gly Tyr Ile Leu Arg
Val Thr Ala Tyr Lys Glu 1010 1015 1020Gly Tyr Gly Glu Gly Cys Val
Thr Ile His Glu Ile Glu Asn Asn 1025 1030 1035Thr Asp Glu Leu Lys
Phe Asn Asn Cys Val Glu Glu Glu Val Tyr 1040 1045 1050Pro Asn Asn
Thr Val Thr Cys Ile Asn Tyr Thr Ala Thr Gln Glu 1055 1060 1065Glu
Tyr Glu Gly Thr Tyr Thr Ser Arg Asn Arg Gly Tyr Asp Glu 1070 1075
1080Ala Tyr Gly Asn Asn Pro Ser Val Pro Ala Asp Tyr Ala Ser Val
1085 1090 1095Tyr Glu Glu Lys Ser Tyr Thr Asp Arg Arg Arg Glu Asn
Pro Cys 1100 1105 1110Glu Ser Asn Arg Gly Tyr Gly Asp Tyr Thr Pro
Leu Pro Ala Gly 1115 1120 1125Tyr Val Thr Lys Glu Leu Glu Tyr Phe
Pro Glu Thr Asp Lys Val 1130 1135 1140Trp Ile Glu Ile Gly Glu Thr
Glu Gly Thr Phe Ile Val Asp Ser 1145 1150 1155Val Glu Leu Leu Leu
Met Glu Glu 1160 1165393501DNAArtificial sequenceSynthetic DNA
sequence designed for plant expression encoding Cry1Da1_7 with an
additional Alanine residue inserted at position 2. 39atggctgaga
tcaacaacca gaaccagtgc gtcccgtaca actgcctgag caaccctaag 60gagatcatcc
tgggtgagga acgcctggag accggcaaca ccgtagccga cattagcctg
120ggcctcatca acttcctcta cagcaacttc gtgcccggcg gtggcttcat
cgtgggcctc 180ctggagctta tctggggctt catcggcccg tcccagtggg
acatcttcct cgcccagatc 240gagcaactga tcagccagcg gatcgaggag
ttcgctagga accaggccat ctcccgcctg 300gagggactct ccaacctcta
caaggtgtac gtgcgcgcgt tcagcgactg ggagaaggac 360ccgaccaacc
cggccctccg cgaggaaatg cgtatccagt tcaacgatat gaactcggcc
420ctcatcaccg ccatcccgct cttccgcgtg cagaactacg aggtggccct
cctgtccgtg 480tacgttcaag ccgccaacct ccacctctcc atcctccgcg
acgtgagcgt gttcggcgag 540cgctggggct acgacaccgc caccatcaac
aaccgctact ccgacctcac ctccctcatc 600cacgtttaca ccaaccactg
cgtggacacg tacaaccagg gcctccgccg cctggagggc 660cgcttcctct
ccgactggat cgtgtacaac cgcttccgcc gccagctcac catctccgtc
720ctggacatcg tcgccttctt tcccaactac gacatccgca cctaccctat
ccagaccgcc 780acccagctca cccgcgaggt ctacctcgac ctcccgttca
tcaacgagaa cctcagcccg 840gccgccgtct acccgacctt ctccgccgct
gagtccgcca tcattcgcag cccgcacctc 900gtggacttcc tcaactcctt
caccatctac accgactccc tcgcccgcag cgcctactgg 960ggcggtcacc
tcgtgaactc cttccgcacc ggcaccacta ccaacctcat ccgcagcccg
1020ctctacggcc gcgagggcaa caccgagcgc ccggtgacca tcaccgccag
cccgagcgtg 1080cccatcttcc gcaccctcag ctaccccacc ggcctggaca
acagcaaccc tgtggcgggc 1140atcgagggcg tggagttcca gaacaccatc
tccaggagca tctaccgcaa gagcggccct 1200atcgacagct tcagcgagct
gcctcctcag gacgccagcg tgagccctgc catcggctac 1260agccacaggc
tgtgccacgc caccttcctg gagcgcatca gcggccctcg catcgcgggc
1320accgtgttct cgtggaccca ccgcagcgcc tctcctacga acgaggtgtc
tcctagtcgc 1380atcacccaga tcccttgggt caaggcccac accctggcta
gtggcgctag tgtcatcaag 1440ggccctggct tcaccggtgg tgacatcctg
accaggaact ctatgggcga gctgggcact 1500ctgagggtca ctttcactgg
ccgcctgcct cagtcttact acatccgctt ccgctacgct 1560agtgtcgcta
accgctctgg tactttccgc tactctcagc ctccgtctta cggtatctct
1620ttccctaaga ctatggacgc tggtgagcct ctgaccagta ggagcttcgc
tcacactact 1680ctgttcactc ctatcacttt ctctagggct caggaggagt
tcgacctgta catccagtct 1740ggtgtgtaca tcgacaggat cgagttcatc
cccgtgaccg ccacgttcga ggccgagtac 1800gaccttgagc gcgcccagaa
ggtggtgaac gccctcttca ctagcactaa ccagctaggc 1860ctgaagactg
acgtgaccga ctaccacatc gaccaagtga gcaacctagt ggcctgcctc
1920tccgacgagt tctgcctcga cgagaagcgc gagctgtccg agaaggtgaa
gcacgccaag 1980cgcctctccg acgagcgcaa cctgctccag gaccccaact
tcaggggcat caacaggcag 2040cccgaccgcg gctggcgcgg ctccaccgac
atcaccatcc agggcggtga cgacgtattc 2100aaggagaact acgttaccct
ccccggcacc ttcgacgagt gttaccccac ctacctctac 2160cagaagatcg
acgagtccaa gctgaaggcc tacacccgct accagctccg cggctacatc
2220gaggactccc aggacctgga aatctacctc atccgctaca acgccaagca
cgagatcgtg 2280aacgtgcctg gcaccggcag cctctggcct ctcagcgtgg
agaaccagat cggcccttgc 2340ggcgagccta accgctgcgc ccctcacctc
gagtggaacc ctgacctcca ctgctcgtgc 2400agggacggcg agaagtgcgc
ccaccatagc caccacttct ctctggacat cgacgtgggc 2460tgcaccgacc
tgaacgagga cctgggcgtg tgggttatct tcaagatcaa gacccaggac
2520ggtcacgcca ggctgggtaa cctggagttc cttgaggaaa agcctctgct
gggtgaggcc 2580ctggccaggg tcaagagggc tgagaagaaa tggagggata
agagggagac cctgcagctg 2640gagaccacta tcgtctacaa ggaggctaag
gagtctgtcg atgctctgtt cgtcaactct 2700cagtacgata gactgcaagc
tgataccaac atcgctatga tccacgctgc ggataagcgg 2760gtccaccgga
tccgggaggc ttaccttccg gagctttctg tcatcccggg tgtcaacgct
2820gcgatcttcg aggaacttga ggaacggatc ttcactgcgt ttagtcttta
cgatgcgcgg 2880aacatcatca agaacgggga cttcaacaat ggtctgctgt
gctggaacgt caagggtcat 2940gtcgaggtcg aggaacaaaa caatcatcgt
agtgtccttg tcattcctga gtgggaggcg 3000gaggtctctc aagaggtccg
tgtttgcccg gggcgtgggt acattcttcg tgttactgcg 3060tacaaggagg
ggtacgggga ggggtgcgtt actattcatg agattgagaa caatactgat
3120gagcttaagt tcaacaattg tgttgaggag gaggtttacc cgaacaatac
tgttacgtgc 3180atcaactaca cggcaacgca agaggaatac gaggggacgt
acacctcgcg taatagaggg 3240tatgatgagg cgtacggaaa caacccgtcg
gttccagcag attatgcctc ggtttatgag 3300gagaagtcgt acacggatag
acgacgcgag aatccatgtg agtcaaatcg aggatacgga 3360gattacacac
cattaccagc aggatacgtt acaaaggagt tggaatactt cccggaaaca
3420gataaagttt ggattgaaat cggagaaaca gaaggaacat tcatcgtcga
ctcagtagaa 3480ttgttgttga tggaagaatg a 3501401166PRTArtificial
sequenceAmino acid sequence of the engineered insecticidal protein
Cry1Da1_7 encoded by a synthetic DNA sequence wherein an additional
Alanine residue has been inserted at position 2. 40Met Ala Glu Ile
Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu1 5 10 15Ser Asn Pro
Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly 20 25 30Asn Thr
Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser 35 40 45Asn
Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile 50 55
60Trp Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile65
70 75 80Glu Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln
Ala 85 90 95Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr
Val Arg 100 105 110Ala Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro
Ala Leu Arg Glu 115 120 125Glu Met Arg Ile Gln Phe Asn Asp Met Asn
Ser Ala Leu Ile Thr Ala 130 135 140Ile Pro Leu Phe Arg Val Gln Asn
Tyr Glu Val Ala Leu Leu Ser Val145 150 155 160Tyr Val Gln Ala Ala
Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser 165 170 175Val Phe Gly
Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg 180 185 190Tyr
Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val 195 200
205Asp Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser
210 215 220Asp Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile
Ser Val225 230 235 240Leu Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp
Ile Arg Thr Tyr Pro 245 250 255Ile Gln Thr Ala Thr Gln Leu Thr Arg
Glu Val Tyr Leu Asp Leu Pro 260 265 270Phe Ile Asn Glu Asn Leu Ser
Pro Ala Ala Val Tyr Pro Thr Phe Ser 275 280 285Ala Ala Glu Ser Ala
Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu 290 295 300Asn Ser Phe
Thr Ile Tyr Thr Asp Ser Leu Ala Arg Ser Ala Tyr Trp305 310 315
320Gly Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu
325 330 335Ile Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg
Pro Val 340 345 350Thr Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg
Thr Leu Ser Tyr 355 360 365Pro
Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val 370 375
380Glu Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly
Pro385 390 395 400Ile Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala
Ser Val Ser Pro 405 410 415Ala Ile Gly Tyr Ser His Arg Leu Cys His
Ala Thr Phe Leu Glu Arg 420 425 430Ile Ser Gly Pro Arg Ile Ala Gly
Thr Val Phe Ser Trp Thr His Arg 435 440 445Ser Ala Ser Pro Thr Asn
Glu Val Ser Pro Ser Arg Ile Thr Gln Ile 450 455 460Pro Trp Val Lys
Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys465 470 475 480Gly
Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly 485 490
495Glu Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser
500 505 510Tyr Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser
Gly Thr 515 520 525Phe Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser
Phe Pro Lys Thr 530 535 540Met Asp Ala Gly Glu Pro Leu Thr Ser Arg
Ser Phe Ala His Thr Thr545 550 555 560Leu Phe Thr Pro Ile Thr Phe
Ser Arg Ala Gln Glu Glu Phe Asp Leu 565 570 575Tyr Ile Gln Ser Gly
Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val 580 585 590Thr Ala Thr
Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val 595 600 605Val
Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp 610 615
620Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys
Leu625 630 635 640Ser Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu
Ser Glu Lys Val 645 650 655Lys His Ala Lys Arg Leu Ser Asp Glu Arg
Asn Leu Leu Gln Asp Pro 660 665 670Asn Phe Arg Gly Ile Asn Arg Gln
Pro Asp Arg Gly Trp Arg Gly Ser 675 680 685Thr Asp Ile Thr Ile Gln
Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr 690 695 700Val Thr Leu Pro
Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr705 710 715 720Gln
Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu 725 730
735Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg
740 745 750Tyr Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly
Ser Leu 755 760 765Trp Pro Leu Ser Val Glu Asn Gln Ile Gly Pro Cys
Gly Glu Pro Asn 770 775 780Arg Cys Ala Pro His Leu Glu Trp Asn Pro
Asp Leu His Cys Ser Cys785 790 795 800Arg Asp Gly Glu Lys Cys Ala
His His Ser His His Phe Ser Leu Asp 805 810 815Ile Asp Val Gly Cys
Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val 820 825 830Ile Phe Lys
Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu 835 840 845Glu
Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val 850 855
860Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln
Leu865 870 875 880Glu Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu Ser
Val Asp Ala Leu 885 890 895Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln
Ala Asp Thr Asn Ile Ala 900 905 910Met Ile His Ala Ala Asp Lys Arg
Val His Arg Ile Arg Glu Ala Tyr 915 920 925Leu Pro Glu Leu Ser Val
Ile Pro Gly Val Asn Ala Ala Ile Phe Glu 930 935 940Glu Leu Glu Glu
Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg945 950 955 960Asn
Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn 965 970
975Val Lys Gly His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser Val
980 985 990Leu Val Ile Pro Glu Trp Glu Ala Glu Val Ser Gln Glu Val
Arg Val 995 1000 1005Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr
Ala Tyr Lys Glu 1010 1015 1020Gly Tyr Gly Glu Gly Cys Val Thr Ile
His Glu Ile Glu Asn Asn 1025 1030 1035Thr Asp Glu Leu Lys Phe Asn
Asn Cys Val Glu Glu Glu Val Tyr 1040 1045 1050Pro Asn Asn Thr Val
Thr Cys Ile Asn Tyr Thr Ala Thr Gln Glu 1055 1060 1065Glu Tyr Glu
Gly Thr Tyr Thr Ser Arg Asn Arg Gly Tyr Asp Glu 1070 1075 1080Ala
Tyr Gly Asn Asn Pro Ser Val Pro Ala Asp Tyr Ala Ser Val 1085 1090
1095Tyr Glu Glu Lys Ser Tyr Thr Asp Arg Arg Arg Glu Asn Pro Cys
1100 1105 1110Glu Ser Asn Arg Gly Tyr Gly Asp Tyr Thr Pro Leu Pro
Ala Gly 1115 1120 1125Tyr Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu
Thr Asp Lys Val 1130 1135 1140Trp Ile Glu Ile Gly Glu Thr Glu Gly
Thr Phe Ile Val Asp Ser 1145 1150 1155Val Glu Leu Leu Leu Met Glu
Glu 1160 1165413423DNAArtificial sequenceSynthetic DNA sequence
designed for plant expression encoding TIC844 with an additional
Alanine residue inserted at position 2. 41atggctgaga tcaacaacca
gaaccagtgc gtcccgtaca actgcctgag caaccctaag 60gagatcatcc tgggtgagga
acgcctggag accggcaaca ccgtagccga cattagcctg 120ggcctcatca
acttcctcta cagcaacttc gtgcccggcg gtggcttcat cgtgggcctc
180ctggagctta tctggggctt catcggcccg tcccagtggg acatcttcct
cgcccagatc 240gagcaactga tcagccagcg gatcgaggag ttcgctagga
accaggccat ctcccgcctg 300gagggactct ccaacctcta caaggtgtac
gtgcgcgcgt tcagcgactg ggagaaggac 360ccgaccaacc cggccctccg
cgaggaaatg cgtatccagt tcaacgatat gaactcggcc 420ctcatcaccg
ccatcccgct cttccgcgtg cagaactacg aggtggccct cctgtccgtg
480tacgttcaag ccgccaacct ccacctctcc atcctccgcg acgtgagcgt
gttcggcgag 540cgctggggct acgacaccgc caccatcaac aaccgctact
ccgacctcac ctccctcatc 600cacgtttaca ccaaccactg cgtggacacg
tacaaccagg gcctccgccg cctggagggc 660cgcttcctct ccgactggat
cgtgtacaac cgcttccgcc gccagctcac catctccgtc 720ctggacatcg
tcgccttctt tcccaactac gacatccgca cctaccctat ccagaccgcc
780acccagctca cccgcgaggt ctacctcgac ctcccgttca tcaacgagaa
cctcagcccg 840gccgccagct acccgacctt ctccgccgct gagtccgcca
tcattcgcag cccgcacctc 900gtggacttcc tcaactcctt caccatctac
accgactccc tcgcccgcta cgcctactgg 960ggcggtcacc tcgtgaactc
cttccgcacc ggcaccacta ccaacctcat ccgcagcccg 1020ctctacggcc
gcgagggcaa caccgagcgc ccggtgacca tcaccgccag cccgagcgtg
1080cccatcttcc gcaccctcag ctacatcacc ggcctggaca acagcaaccc
tgtggcgggc 1140atcgagggcg tggagttcca gaacaccatc tccaggagca
tctaccgcaa gagcggccct 1200atcgacagct tcagcgagct gcctcctcag
gacgccagcg tgagccctgc catcggctac 1260agccacaggc tgtgccacgc
caccttcctg gagcgcatca gcggccctcg catcgcgggc 1320accgtgttct
cgtggaccca ccgcagcgcc tctcctacga acgaggtgtc tcctagtcgc
1380atcacccaga tcccttgggt caaggcccac accctggcta gtggcgctag
tgtcatcaag 1440ggccctggct tcaccggtgg tgacatcctg accaggaact
ctatgggcga gctgggcact 1500ctgagggtca ctttcactgg ccgcctgcct
cagtcttact acatccgctt ccgctacgct 1560agtgtcgcta accgctctgg
tactttccgc tactctcagc ctccgtctta cggtatctct 1620ttccctaaga
ctatggacgc tggtgagcct ctgaccagta ggagcttcgc tcacactact
1680ctgttcactc ctatcacttt ctctagggct caggaggagt tcgacctgta
catccagtct 1740ggtgtgtaca tcgacaggat cgagttcatc cccgtgaccg
ccacgttcga ggccgagtac 1800gaccttgagc gcgcccagaa ggctgtcaat
gagctcttca cgtccagcaa tcagatcggc 1860ctgaagaccg acgtcactga
ctaccacatc gaccaagtct ccaacctcgt ggagtgcctc 1920tccgatgagt
tctgcctcga cgagaagaag gagctgtccg agaaggtgaa gcatgccaag
1980cgtctcagcg acgagaggaa tctcctccag gaccccaatt tccgcggcat
caacaggcag 2040ctcgaccgcg gctggcgcgg cagcaccgac atcacgatcc
agggcggcga cgatgtgttc 2100aaggagaact acgtgactct cctgggcact
ttcgacgagt gctaccctac ctacttgtac 2160cagaagatcg atgagtccaa
gctcaaggct tacactcgct accagctccg cggctacatc 2220gaagacagcc
aagacctcga gatttacctg atccgctaca acgccaagca cgagaccgtc
2280aacgtgcccg gtactggttc cctctggccg ctgagcgccc ccagcccgat
cggcaagtgt 2340gcccaccaca gccaccactt ctccttggac atcgatgtgg
gctgcaccga cctgaacgag 2400gacctcggag tctgggtcat cttcaagatc
aagacccagg acggccacgc gcgcctgggc 2460aacctggagt tcctcgagga
gaagcccctg gtcggtgagg ctctggccag ggtcaagagg 2520gctgagaaga
agtggaggga caagcgcgag aagctcgagt gggagaccaa catcgtttac
2580aaggaggcca aggagagcgt cgacgccctg ttcgtgaact cccagtacga
ccgcctgcag 2640gccgacacca acatcgccat gatccacgct gccgacaaga
gggtgcacag cattcgcgag 2700gcctacctgc ctgagctgtc cgtgatccct
ggtgtgaacg ctgccatctt tgaggagctg 2760gagggccgca tctttaccgc
attctccctg tacgacgccc gcaacgtgat caagaacggt 2820gacttcaaca
atggcctcag ctgctggaac gtcaagggcc acgtggacgt cgaggaacag
2880aacaaccacc gctccgtcct ggtcgtccca gagtgggagg ctgaggtctc
ccaagaggtc 2940cgcgtctgcc caggccgcgg ctacattctc agggtcaccg
cttacaagga gggctacggt 3000gagggctgtg tgaccatcca cgagatcgag
aacaacaccg acgagcttaa gttctccaac 3060tgcgtggagg aggaggtgta
cccaaacaac accgttactt gcaacgacta caccgccacc 3120caggaggagt
acgagggcac ctacacttcc aggaacaggg gctacgatgg tgcctacgag
3180agcaacagca gcgttcctgc tgactacgct tccgcctacg aggagaaggc
ctacacggat 3240ggccgcaggg acaacccttg cgagagcaac cgcggctacg
gcgactacac tcccctgccc 3300gccggctacg ttaccaagga gctggagtac
ttcccggaga ctgacaaggt gtggatcgag 3360atcggcgaga ccgagggcac
cttcatcgtg gacagcgtgg agctgctcct gatggaggag 3420tag
3423421140PRTArtificial sequenceAmino acid sequence of the chimeric
protein TIC844 encoded by a synthetic DNA sequence wherein an
additional Alanine residue has been inserted at position 2. 42Met
Ala Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu1 5 10
15Ser Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly
20 25 30Asn Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr
Ser 35 40 45Asn Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu
Leu Ile 50 55 60Trp Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu
Ala Gln Ile65 70 75 80Glu Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe
Ala Arg Asn Gln Ala 85 90 95Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu
Tyr Lys Val Tyr Val Arg 100 105 110Ala Phe Ser Asp Trp Glu Lys Asp
Pro Thr Asn Pro Ala Leu Arg Glu 115 120 125Glu Met Arg Ile Gln Phe
Asn Asp Met Asn Ser Ala Leu Ile Thr Ala 130 135 140Ile Pro Leu Phe
Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val145 150 155 160Tyr
Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser 165 170
175Val Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg
180 185 190Tyr Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His
Cys Val 195 200 205Asp Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly
Arg Phe Leu Ser 210 215 220Asp Trp Ile Val Tyr Asn Arg Phe Arg Arg
Gln Leu Thr Ile Ser Val225 230 235 240Leu Asp Ile Val Ala Phe Phe
Pro Asn Tyr Asp Ile Arg Thr Tyr Pro 245 250 255Ile Gln Thr Ala Thr
Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro 260 265 270Phe Ile Asn
Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser 275 280 285Ala
Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu 290 295
300Asn Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Ala Tyr
Trp305 310 315 320Gly Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr
Thr Thr Asn Leu 325 330 335Ile Arg Ser Pro Leu Tyr Gly Arg Glu Gly
Asn Thr Glu Arg Pro Val 340 345 350Thr Ile Thr Ala Ser Pro Ser Val
Pro Ile Phe Arg Thr Leu Ser Tyr 355 360 365Ile Thr Gly Leu Asp Asn
Ser Asn Pro Val Ala Gly Ile Glu Gly Val 370 375 380Glu Phe Gln Asn
Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro385 390 395 400Ile
Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro 405 410
415Ala Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg
420 425 430Ile Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr
His Arg 435 440 445Ser Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg
Ile Thr Gln Ile 450 455 460Pro Trp Val Lys Ala His Thr Leu Ala Ser
Gly Ala Ser Val Ile Lys465 470 475 480Gly Pro Gly Phe Thr Gly Gly
Asp Ile Leu Thr Arg Asn Ser Met Gly 485 490 495Glu Leu Gly Thr Leu
Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser 500 505 510Tyr Tyr Ile
Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr 515 520 525Phe
Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr 530 535
540Met Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr
Thr545 550 555 560Leu Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu
Glu Phe Asp Leu 565 570 575Tyr Ile Gln Ser Gly Val Tyr Ile Asp Arg
Ile Glu Phe Ile Pro Val 580 585 590Thr Ala Thr Phe Glu Ala Glu Tyr
Asp Leu Glu Arg Ala Gln Lys Ala 595 600 605Val Asn Glu Leu Phe Thr
Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp 610 615 620Val Thr Asp Tyr
His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu625 630 635 640Ser
Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val 645 650
655Lys His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro
660 665 670Asn Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg
Gly Ser 675 680 685Thr Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe
Lys Glu Asn Tyr 690 695 700Val Thr Leu Leu Gly Thr Phe Asp Glu Cys
Tyr Pro Thr Tyr Leu Tyr705 710 715 720Gln Lys Ile Asp Glu Ser Lys
Leu Lys Ala Tyr Thr Arg Tyr Gln Leu 725 730 735Arg Gly Tyr Ile Glu
Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg 740 745 750Tyr Asn Ala
Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu 755 760 765Trp
Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Ala His His Ser 770 775
780His His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn
Glu785 790 795 800Asp Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr
Gln Asp Gly His 805 810 815Ala Arg Leu Gly Asn Leu Glu Phe Leu Glu
Glu Lys Pro Leu Val Gly 820 825 830Glu Ala Leu Ala Arg Val Lys Arg
Ala Glu Lys Lys Trp Arg Asp Lys 835 840 845Arg Glu Lys Leu Glu Trp
Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys 850 855 860Glu Ser Val Asp
Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln865 870 875 880Ala
Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg Val His 885 890
895Ser Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val
900 905 910Asn Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile Phe Thr
Ala Phe 915 920 925Ser Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn Gly
Asp Phe Asn Asn 930 935 940Gly Leu Ser Cys Trp Asn Val Lys Gly His
Val Asp Val Glu Glu Gln945 950 955 960Asn Asn His Arg Ser Val Leu
Val Val Pro Glu Trp Glu Ala Glu Val 965 970 975Ser Gln Glu Val Arg
Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val 980 985 990Thr Ala Tyr
Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile His Glu 995 1000
1005Ile Glu Asn Asn Thr Asp Glu Leu Lys Phe Ser Asn Cys Val Glu
1010 1015 1020Glu Glu Val Tyr Pro Asn Asn Thr Val Thr Cys Asn Asp
Tyr Thr 1025 1030 1035Ala Thr Gln Glu Glu Tyr Glu Gly Thr Tyr
Thr
Ser Arg Asn Arg 1040 1045 1050Gly Tyr Asp Gly Ala Tyr Glu Ser Asn
Ser Ser Val Pro Ala Asp 1055 1060 1065Tyr Ala Ser Ala Tyr Glu Glu
Lys Ala Tyr Thr Asp Gly Arg Arg 1070 1075 1080Asp Asn Pro Cys Glu
Ser Asn Arg Gly Tyr Gly Asp Tyr Thr Pro 1085 1090 1095Leu Pro Ala
Gly Tyr Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu 1100 1105 1110Thr
Asp Lys Val Trp Ile Glu Ile Gly Glu Thr Glu Gly Thr Phe 1115 1120
1125Ile Val Asp Ser Val Glu Leu Leu Leu Met Glu Glu 1130 1135
1140433423DNAArtificialSynthetic DNA sequence designed for plant
expression encoding TIC844_8 with an additional Alanine residue
inserted at position 2. 43atggctgaga tcaacaacca gaaccagtgc
gtcccgtaca actgcctgag caaccctaag 60gagatcatcc tgggtgagga acgcctggag
accggcaaca ccgtagccga cattagcctg 120ggcctcatca acttcctcta
cagcaacttc gtgcccggcg gtggcttcat cgtgggcctc 180ctggagctta
tctggggctt catcggcccg tcccagtggg acatcttcct cgcccagatc
240gagcaactga tcagccagcg gatcgaggag ttcgctagga accaggccat
ctcccgcctg 300gagggactct ccaacctcta caaggtgtac gtgcgcgcgt
tcagcgactg ggagaaggac 360ccgaccaacc cggccctccg cgaggaaatg
cgtatccagt tcaacgatat gaactcggcc 420ctcatcaccg ccatcccgct
cttccgcgtg cagaactacg aggtggccct cctgtccgtg 480tacgttcaag
ccgccaacct ccacctctcc atcctccgcg acgtgagcgt gttcggcgag
540cgctggggct acgacaccgc caccatcaac aaccgctact ccgacctcac
ctccctcatc 600cacgtttaca ccaaccactg cgtggacacg tacaaccagg
gcctccgccg cctggagggc 660cgcttcctct ccgactggat cgtgtacaac
cgcttccgcc gccagctcac catctccgtc 720ctggacatcg tcgccttctt
tcccaactac gacatccgca cctaccctat ccagaccgcc 780acccagctca
cccgcgaggt ctacctcgac ctcccgttca tcaacgagaa cctcagcccg
840gccgccgtct acccgacctt ctccgccgct gagtccgcca tcattcgcag
cccgcacctc 900gtggacttcc tcaactcctt caccatctac accgactccc
tcgcccgcag cgcctactgg 960ggcggtcacc tcgtgaactc cttccgcacc
ggcaccacta ccaacctcat ccgcagcccg 1020ctctacggcc gcgagggcaa
caccgagcgc ccggtgacca tcaccgccag cccgagcgtg 1080cccatcttcc
gcaccctcag ctaccccacc ggcctggaca acagcaaccc tgtggcgggc
1140atcgagggcg tggagttcca gaacaccatc tccaggagca tctaccgcaa
gagcggccct 1200atcgacagct tcagcgagct gcctcctcag gacgccagcg
tgagccctgc catcggctac 1260agccacaggc tgtgccacgc caccttcctg
gagcgcatca gcggccctcg catcgcgggc 1320accgtgttct cgtggaccca
ccgcagcgcc tctcctacga acgaggtgtc tcctagtcgc 1380atcacccaga
tcccttgggt caaggcccac accctggcta gtggcgctag tgtcatcaag
1440ggccctggct tcaccggtgg tgacatcctg accaggaact ctatgggcga
gctgggcact 1500ctgagggtca ctttcactgg ccgcctgcct cagtcttact
acatccgctt ccgctacgct 1560agtgtcgcta accgctctgg tactttccgc
tactctcagc ctccgtctta cggtatctct 1620ttccctaaga ctatggacgc
tggtgagcct ctgaccagta ggagcttcgc tcacactact 1680ctgttcactc
ctatcacttt ctctagggct caggaggagt tcgacctgta catccagtct
1740ggtgtgtaca tcgacaggat cgagttcatc cccgtgaccg ccacgttcga
ggccgagtac 1800gaccttgagc gcgcccagaa ggctgtcaat gagctcttca
cgtccagcaa tcagatcggc 1860ctgaagaccg acgtcactga ctaccacatc
gaccaagtct ccaacctcgt ggagtgcctc 1920tccgatgagt tctgcctcga
cgagaagaag gagctgtccg agaaggtgaa gcatgccaag 1980cgtctcagcg
acgagaggaa tctcctccag gaccccaatt tccgcggcat caacaggcag
2040ctcgaccgcg gctggcgcgg cagcaccgac atcacgatcc agggcggcga
cgatgtgttc 2100aaggagaact acgtgactct cctgggcact ttcgacgagt
gctaccctac ctacttgtac 2160cagaagatcg atgagtccaa gctcaaggct
tacactcgct accagctccg cggctacatc 2220gaagacagcc aagacctcga
gatttacctg atccgctaca acgccaagca cgagaccgtc 2280aacgtgcccg
gtactggttc cctctggccg ctgagcgccc ccagcccgat cggcaagtgt
2340gcccaccaca gccaccactt ctccttggac atcgatgtgg gctgcaccga
cctgaacgag 2400gacctcggag tctgggtcat cttcaagatc aagacccagg
acggccacgc gcgcctgggc 2460aacctggagt tcctcgagga gaagcccctg
gtcggtgagg ctctggccag ggtcaagagg 2520gctgagaaga agtggaggga
caagcgcgag aagctcgagt gggagaccaa catcgtttac 2580aaggaggcca
aggagagcgt cgacgccctg ttcgtgaact cccagtacga ccgcctgcag
2640gccgacacca acatcgccat gatccacgct gccgacaaga gggtgcacag
cattcgcgag 2700gcctacctgc ctgagctgtc cgtgatccct ggtgtgaacg
ctgccatctt tgaggagctg 2760gagggccgca tctttaccgc attctccctg
tacgacgccc gcaacgtgat caagaacggt 2820gacttcaaca atggcctcag
ctgctggaac gtcaagggcc acgtggacgt cgaggaacag 2880aacaaccacc
gctccgtcct ggtcgtccca gagtgggagg ctgaggtctc ccaagaggtc
2940cgcgtctgcc caggccgcgg ctacattctc agggtcaccg cttacaagga
gggctacggt 3000gagggctgtg tgaccatcca cgagatcgag aacaacaccg
acgagcttaa gttctccaac 3060tgcgtggagg aggaggtgta cccaaacaac
accgttactt gcaacgacta caccgccacc 3120caggaggagt acgagggcac
ctacacttcc aggaacaggg gctacgatgg tgcctacgag 3180agcaacagca
gcgttcctgc tgactacgct tccgcctacg aggagaaggc ctacacggat
3240ggccgcaggg acaacccttg cgagagcaac cgcggctacg gcgactacac
tcccctgccc 3300gccggctacg ttaccaagga gctggagtac ttcccggaga
ctgacaaggt gtggatcgag 3360atcggcgaga ccgagggcac cttcatcgtg
gacagcgtgg agctgctcct gatggaggag 3420tag
3423441140PRTArtificialAmino acid sequence of the engineered
insecticidal chimeric protein TIC844_8 encoded by a synthetic DNA
sequence wherein an additional Alanine residue has been inserted at
position 2. 44Met Ala Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr
Asn Cys Leu1 5 10 15Ser Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg
Leu Glu Thr Gly 20 25 30Asn Thr Val Ala Asp Ile Ser Leu Gly Leu Ile
Asn Phe Leu Tyr Ser 35 40 45Asn Phe Val Pro Gly Gly Gly Phe Ile Val
Gly Leu Leu Glu Leu Ile 50 55 60Trp Gly Phe Ile Gly Pro Ser Gln Trp
Asp Ile Phe Leu Ala Gln Ile65 70 75 80Glu Gln Leu Ile Ser Gln Arg
Ile Glu Glu Phe Ala Arg Asn Gln Ala 85 90 95Ile Ser Arg Leu Glu Gly
Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg 100 105 110Ala Phe Ser Asp
Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu 115 120 125Glu Met
Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala 130 135
140Ile Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser
Val145 150 155 160Tyr Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu
Arg Asp Val Ser 165 170 175Val Phe Gly Glu Arg Trp Gly Tyr Asp Thr
Ala Thr Ile Asn Asn Arg 180 185 190Tyr Ser Asp Leu Thr Ser Leu Ile
His Val Tyr Thr Asn His Cys Val 195 200 205Asp Thr Tyr Asn Gln Gly
Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser 210 215 220Asp Trp Ile Val
Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val225 230 235 240Leu
Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro 245 250
255Ile Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro
260 265 270Phe Ile Asn Glu Asn Leu Ser Pro Ala Ala Val Tyr Pro Thr
Phe Ser 275 280 285Ala Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu
Val Asp Phe Leu 290 295 300Asn Ser Phe Thr Ile Tyr Thr Asp Ser Leu
Ala Arg Ser Ala Tyr Trp305 310 315 320Gly Gly His Leu Val Asn Ser
Phe Arg Thr Gly Thr Thr Thr Asn Leu 325 330 335Ile Arg Ser Pro Leu
Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val 340 345 350Thr Ile Thr
Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr 355 360 365Pro
Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val 370 375
380Glu Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly
Pro385 390 395 400Ile Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala
Ser Val Ser Pro 405 410 415Ala Ile Gly Tyr Ser His Arg Leu Cys His
Ala Thr Phe Leu Glu Arg 420 425 430Ile Ser Gly Pro Arg Ile Ala Gly
Thr Val Phe Ser Trp Thr His Arg 435 440 445Ser Ala Ser Pro Thr Asn
Glu Val Ser Pro Ser Arg Ile Thr Gln Ile 450 455 460Pro Trp Val Lys
Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys465 470 475 480Gly
Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly 485 490
495Glu Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser
500 505 510Tyr Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser
Gly Thr 515 520 525Phe Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser
Phe Pro Lys Thr 530 535 540Met Asp Ala Gly Glu Pro Leu Thr Ser Arg
Ser Phe Ala His Thr Thr545 550 555 560Leu Phe Thr Pro Ile Thr Phe
Ser Arg Ala Gln Glu Glu Phe Asp Leu 565 570 575Tyr Ile Gln Ser Gly
Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val 580 585 590Thr Ala Thr
Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala 595 600 605Val
Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp 610 615
620Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys
Leu625 630 635 640Ser Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu
Ser Glu Lys Val 645 650 655Lys His Ala Lys Arg Leu Ser Asp Glu Arg
Asn Leu Leu Gln Asp Pro 660 665 670Asn Phe Arg Gly Ile Asn Arg Gln
Leu Asp Arg Gly Trp Arg Gly Ser 675 680 685Thr Asp Ile Thr Ile Gln
Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr 690 695 700Val Thr Leu Leu
Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr705 710 715 720Gln
Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu 725 730
735Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg
740 745 750Tyr Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly
Ser Leu 755 760 765Trp Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys
Ala His His Ser 770 775 780His His Phe Ser Leu Asp Ile Asp Val Gly
Cys Thr Asp Leu Asn Glu785 790 795 800Asp Leu Gly Val Trp Val Ile
Phe Lys Ile Lys Thr Gln Asp Gly His 805 810 815Ala Arg Leu Gly Asn
Leu Glu Phe Leu Glu Glu Lys Pro Leu Val Gly 820 825 830Glu Ala Leu
Ala Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys 835 840 845Arg
Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys 850 855
860Glu Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu
Gln865 870 875 880Ala Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp
Lys Arg Val His 885 890 895Ser Ile Arg Glu Ala Tyr Leu Pro Glu Leu
Ser Val Ile Pro Gly Val 900 905 910Asn Ala Ala Ile Phe Glu Glu Leu
Glu Gly Arg Ile Phe Thr Ala Phe 915 920 925Ser Leu Tyr Asp Ala Arg
Asn Val Ile Lys Asn Gly Asp Phe Asn Asn 930 935 940Gly Leu Ser Cys
Trp Asn Val Lys Gly His Val Asp Val Glu Glu Gln945 950 955 960Asn
Asn His Arg Ser Val Leu Val Val Pro Glu Trp Glu Ala Glu Val 965 970
975Ser Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val
980 985 990Thr Ala Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile
His Glu 995 1000 1005Ile Glu Asn Asn Thr Asp Glu Leu Lys Phe Ser
Asn Cys Val Glu 1010 1015 1020Glu Glu Val Tyr Pro Asn Asn Thr Val
Thr Cys Asn Asp Tyr Thr 1025 1030 1035Ala Thr Gln Glu Glu Tyr Glu
Gly Thr Tyr Thr Ser Arg Asn Arg 1040 1045 1050Gly Tyr Asp Gly Ala
Tyr Glu Ser Asn Ser Ser Val Pro Ala Asp 1055 1060 1065Tyr Ala Ser
Ala Tyr Glu Glu Lys Ala Tyr Thr Asp Gly Arg Arg 1070 1075 1080Asp
Asn Pro Cys Glu Ser Asn Arg Gly Tyr Gly Asp Tyr Thr Pro 1085 1090
1095Leu Pro Ala Gly Tyr Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu
1100 1105 1110Thr Asp Lys Val Trp Ile Glu Ile Gly Glu Thr Glu Gly
Thr Phe 1115 1120 1125Ile Val Asp Ser Val Glu Leu Leu Leu Met Glu
Glu 1130 1135 1140
uspto.report is an independent third-party trademark research tool that is not affiliated, endorsed, or sponsored by the United States Patent and Trademark Office (USPTO) or any other governmental organization. The information provided by uspto.report is based on publicly available data at the time of writing and is intended for informational purposes only.
While we strive to provide accurate and up-to-date information, we do not guarantee the accuracy, completeness, reliability, or suitability of the information displayed on this site. The use of this site is at your own risk. Any reliance you place on such information is therefore strictly at your own risk.
All official trademark data, including owner information, should be verified by visiting the official USPTO website at www.uspto.gov. This site is not intended to replace professional legal advice and should not be used as a substitute for consulting with a legal professional who is knowledgeable about trademark law.