Compositions And Methods To Control Insect Pests
HU; XU ; et al.
U.S. patent application number 15/736369 was filed with the patent office on 2018-06-07 for compositions and methods to control insect pests. This patent application is currently assigned to PIONEER HI-BRED INTERNATIONAL, INC.. The applicant listed for this patent is E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.. Invention is credited to XU HU, ADANE KASSA, BLISS MARIE KERNODLE, ALBERT L. LU, MOLLY MARY MCMAHON, XIPING NIU, JAMES KEVIN PRESNAIL, NINA RICHTMAN, JONATHAN WILLIAM ROBESON.
| Application Number | 20180155739 15/736369 |
| Document ID | / |
| Family ID | 56204060 |
| Filed Date | 2018-06-07 |
| United States Patent Application | 20180155739 |
| Kind Code | A1 |
| HU; XU ; et al. | June 7, 2018 |
COMPOSITIONS AND METHODS TO CONTROL INSECT PESTS
Abstract
Methods and compositions are provided which employ a silencing element that, when ingested by a plant insect pest, such as Coleopteran, Hemiptera, or Lepidopteran plant pest, including a Diabrotica, Leptinotarsa, Phyllotreta, Acyrthosiphan, Bemisia, Halyomorpha, Nezara, or Spodoptera plant pest, decrease the expression of a target sequence in the pest. Disclosed are various target polynucleotides set forth in any one of SEQ ID NOS: 1-53 or 107-254 disclosed herein, or variants and fragments thereof, or complements thereof, wherein a decrease in expression of one or more of the sequences in the target pest controls the pest and pest population by insect sterilization (i.e., male and female sterility, reduction of sperm count, egg production and viability) and mating-based sterile insect technique (SIT). Plants, plant parts, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.
| Inventors: | HU; XU; (JOHNSTON, IA) ; KERNODLE; BLISS MARIE; (JOHNSTON, IA) ; MCMAHON; MOLLY MARY; (DES MOINES, IA) ; KASSA; ADANE; (JOHNSTON, IA) ; NIU; XIPING; (JOHNSTON, IA) ; PRESNAIL; JAMES KEVIN; (ST LOUIS, MO) ; LU; ALBERT L.; (WEST DES MOINES, IA) ; RICHTMAN; NINA; (JOHNSTON, IA) ; ROBESON; JONATHAN WILLIAM; (URBANDALE, IA) | ||||||||||
| Applicant: |
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| Assignee: | PIONEER HI-BRED INTERNATIONAL,
INC. JOHNSTON IA E. I. DU PONT DE NEMOURS AND COMPANY WILMINGTON DE |
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| Family ID: | 56204060 | ||||||||||
| Appl. No.: | 15/736369 | ||||||||||
| Filed: | June 16, 2016 | ||||||||||
| PCT Filed: | June 16, 2016 | ||||||||||
| PCT NO: | PCT/US2016/037748 | ||||||||||
| 371 Date: | December 14, 2017 |
Related U.S. Patent Documents
| Application Number | Filing Date | Patent Number | ||
|---|---|---|---|---|
| 62180504 | Jun 16, 2015 | |||
| 62272994 | Dec 30, 2015 | |||
| Current U.S. Class: | 1/1 |
| Current CPC Class: | C07K 14/43563 20130101; C12N 15/8218 20130101; A01N 65/00 20130101; C07K 14/43536 20130101; A01N 63/30 20200101; C12N 15/8286 20130101; C12N 2310/14 20130101; A01N 37/46 20130101; Y02A 40/146 20180101; A01N 63/10 20200101; C12N 2310/531 20130101; C12N 15/113 20130101; Y02A 40/162 20180101 |
| International Class: | C12N 15/82 20060101 C12N015/82; C12N 15/113 20060101 C12N015/113 |
Claims
1. A silencing element comprising at least one double-stranded RNA region, at least one strand of which comprises a polynucleotide that is complementary to: (a) the nucleotide sequence comprising any one of SEQ ID NOS: 1-53 or 107-253; or variants and fragments thereof, and complements thereof; (b) the nucleotide sequence comprising at least 90% sequence identity to any one of nucleotides SEQ ID NOS: 1-53 or 107-253; or variants and fragments thereof, and complements thereof; or (c) the nucleotide sequence comprising at least 19 consecutive nucleotides of any one of SEQ ID NOS: 1-53 or 107-253; or variants and fragments thereof, and complements thereof; wherein the silencing element has sterilization activity against an insect plant pest.
2. (canceled)
3. The silencing element of claim 1, wherein the insect plant pest is a Coleoptera plant pest.
4. The silencing element of claim 3, wherein the Coleoptera plant pest is a Diabrotica plant pest.
5. The silencing element of claim 4, wherein the Diabrotica plant pest comprises D. virgifera virgifera, D. virgifera zeae, D. speciosa, D. barberi, D. virgifera zeae, or D. undecimpunctata howardi.
6-19. (canceled)
20. The silencing element of claim 1, wherein the silencing element comprises a hairpin loop.
21. The silencing element of claim 20, wherein the silencing element comprises, a first segment, a second segment, and a third segment, wherein a. the first segment comprises at least about 19 nucleotides having at least 90% sequence complementarity to a sequence set forth in any one of SEQ ID NOS: 1-53 or 107-253; or variants and fragments, and complements thereof; or the first segment consists of at least 19 nucleotides having at least 90% sequence complementarity to a sequence set forth in any one of SEQ ID NOS: 1-53 or 107-253; b. the second segment comprises a loop of sufficient length to allow the silencing element to be transcribed as a hairpin RNA; and, c. the third segment comprises at least about 19 nucleotides having at least 85% complementarity to the first segment; wherein the second segment that is not complementary a sequence set forth in any one of SEQ ID NOS: 1-53 or 107-253, or to the other segments of the construct; and wherein the first and third segments form at least a partially double-stranded region.
22-25. (canceled)
26. The silencing element of claim 21, wherein the first segment is complementary to a Coleoptera insect species; and wherein the third segment is complementary to a different Coleoptera insect species.
27. (canceled)
28. (canceled)
29. A DNA construct comprising a polynucleotide encoding the silencing element of claim 1.
30. An expression construct comprising a DNA construct of claim 29.
31. The expression cassette of claim 30, wherein the polynucleotide is operably linked to a heterologous promoter.
32. The expression cassette of claim 30, wherein the polynucleotide is flanked by a first operably linked convergent promoter at one terminus of the polynucleotide and a second operably linked convergent promoter at the opposing terminus of the polynucleotide, wherein the first and the second convergent promoters are capable of driving expression of the silencing element.
33. A host cell comprising the DNA construct of claim 29.
34. The host cell of claim 33, wherein the host cell is a bacterial cell.
35. The host cell of claim 34, wherein the bacterial cell is an inactivated bacterial cell.
36. A host cell comprising the expression construct of claim 30.
37. (canceled)
38. (canceled)
39. A composition comprising the ribonucleic acid construct of claim 1.
40. The composition of claim 39, further comprising an agriculturally acceptable carrier.
41. The composition of claim 39, further comprising a herbicide compound, an insecticide, a fungicide, a nematocide, an agriculturally-acceptable carrier, and/or a bacteria, or combinations thereof.
42. The composition of claim 39, wherein the composition is in liquid form, solid form, or gel form.
43. (canceled)
44. The composition of claim 42, wherein the solid form is a pellet, a powder, an aggregate, or a molded article.
45. A plant cell having stably incorporated into its genome a heterologous polynucleotide encoding a silencing element, wherein the polynucleotide comprises: a. the nucleotide sequence comprising any one of SEQ ID NOS: 1-53 or 107-253; or variants and fragments thereof, and complements thereof; b. the nucleotide sequence comprising at least 90% sequence identity to any one of nucleotides SEQ ID NOS: 1-53 or 107-253; or variants and fragments thereof, and complements thereof; or c. the nucleotide sequence comprising at least 19 consecutive nucleotides of any one of SEQ ID NOS: 1-53 or 107-253; or variants and fragments thereof, and complements thereof; wherein the silencing element decreases the fertility of an insect plant pest.
46. (canceled)
47. The plant cell of claim 45, wherein the insect plant pest is a Coleoptera plant pest.
48. The plant cell of claim 47, wherein the Coleoptera plant pest is a Diabrotica plant pest.
49. The plant cell of claim 48, wherein the Diabrotica plant pest comprises D. virgifera virgifera, D. virgifera zeae, D. speciosa, D. barberi, D. virgifera zeae, or D. undecimpunctata howardi.
50.-63. (canceled)
64. The plant cell of claim 45, wherein the plant cell further comprises an expression cassette, wherein the expression cassette comprises the heterologous polynucleotide encoding a silencing element.
65. The plant cell of claim 45, wherein the silencing element expresses a double stranded RNA.
66. The plant cell of claim 45, wherein the silencing element expresses a hairpin RNA.
67. The plant cell of claim 45, wherein the polynucleotide is operably linked to a heterologous promoter.
68. The plant cell of claim 45, wherein the plant cell is from a monocot.
69. The plant cell of claim 68, wherein the monocot is maize, barley, millet, wheat or rice.
70. The plant cell of claim 45, wherein the plant cell is from a dicot.
71. The plant cell of claim 70, wherein the dicot is kale, cauliflower, broccoli, mustard plant, cabbage, pea, clover, alfalfa, broad bean, tomato, cassava, soybean, canola, alfalfa, sunflower, safflower, tobacco, Arabidopsis, or cotton.
72. A plant or plant part comprising the plant cell of claim 45.
73. A transgenic seed from the plant of claim 72.
74. A method for controlling a plant insect pest comprising feeding to a plant insect pest a composition comprising a silencing element, wherein the silencing element controls the plant pest, wherein the silencing element comprises a sequence complementary to: a. the nucleotide sequence comprising any one of SEQ ID NOS: 1-53 or 107-253; or variants and fragments thereof, and complements thereof; b. the nucleotide sequence comprising at least 90% sequence identity to any one of nucleotides SEQ ID NOS: 1-53 or 107-253; or variants and fragments thereof, and complements thereof; or c. the nucleotide sequence comprising at least 19 consecutive nucleotides of any one of SEQ ID NOS: 1-53 or 107-253; or variants and fragments thereof, and complements thereof; or wherein the silencing element has insect sterilization activity against the plant pest.
75. The method of claim 74, wherein the composition comprises a plant or plant part having stably incorporated into its genome a polynucleotide encoding the silencing element.
76. The method of claim 74, wherein the silencing element comprises a double stranded RNA.
77. The method of claim 74, wherein the silencing element comprises a hairpin RNA.
78. The method of claim 75, wherein the polynucleotide encoding silencing element is operably linked to a heterologous promoter.
79. (canceled)
80. The method of claim 74, wherein the silencing element comprises, a first segment, a second segment, and a third segment, wherein a. the first segment comprises at least about 19 nucleotides having at least 90% sequence complementarity to a sequence set forth in any one of SEQ ID NOS: 1-53 or 107-253; or variants and fragments, and complements thereof; b. the second segment comprises a loop of sufficient length to allow the silencing element to be transcribed as a hairpin RNA; and, c. the third segment comprises at least about 19 nucleotides having at least 85% complementarity to the first segment; wherein the second segment that is not complementary a sequence set forth in any one of SEQ ID NOS: 1-53 or 107-253; or to the other segments of the construct; and wherein the first and third segments form at least a partially double-stranded region.
81-84. (canceled)
85. The method of claim 80, wherein the first segment is complementary to a Coleoptera insect species; and wherein the third segment is complementary to a different Coleoptera insect species.
86. (canceled)
87. (canceled)
88. The method of claim 74, wherein the plant is a monocot.
89. The method of claim 88, wherein the monocot is maize, barley, millet, wheat or rice.
90. The method of claim 74, wherein the plant is a dicot.
91. The method of claim 90, wherein the dicot is kale, cauliflower, broccoli, mustard plant, cabbage, pea, clover, alfalfa, broad bean, tomato, cassava, soybean, canola, alfalfa, sunflower, safflower, tobacco, Arabidopsis, or cotton.
92-110. (canceled)
Description
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of International Application Number PCT/US2016/037748 filed Jun. 16, 2016, which claims the benefit of U.S. Provisional Application No. 62/180,504, filed Jun. 16, 2015, and U.S. Provisional Application No. 62/272,994, filed Dec. 30, 2015, which are hereby incorporated herein in its entirety by reference.
REFERENCE TO SEQUENCE LISTING SUBMITTED ELECTRONICALLY
[0002] A sequence listing having the file name "6030WOPCT_SequenceList.txt" created on May 19, 2016 and having a size of 531 kilobytes is filed in computer readable form concurrently with the specification. The sequence listing is part of the specification and is herein incorporated by reference in its entirety.
FIELD OF THE INVENTION
[0003] The present invention relates generally to methods of molecular biology and gene silencing to control pests.
BACKGROUND
[0004] Plant insect pests are a serious problem in agriculture. They destroy millions of acres of staple crops such as corn, soybeans, peas, and cotton. Yearly, plant insect pests cause over $100 billion dollars in crop damage in the U.S. alone. In an ongoing seasonal battle, farmers must apply billions of gallons of synthetic pesticides to combat these pests. Other methods employed in the past delivered insecticidal activity by microorganisms or genes derived from microorganisms expressed in transgenic plants. For example, certain species of microorganisms of the genus Bacillus are known to possess pesticidal activity against a broad range of insect pests including Lepidoptera, Diptera, Coleoptera, Hemiptera, and others. In fact, microbial pesticides, particularly those obtained from Bacillus strains, have played an important role in agriculture as alternatives to chemical pest control. Agricultural scientists have developed crop plants with enhanced insect resistance by genetically engineering crop plants to produce insecticidal proteins from Bacillus. For example, corn and cotton plants genetically engineered to produce Cry toxins (see, e.g., Aronson (2002) Cell Mol. Life Sci. 59(3):417-425; Schnepf et al. (1998) Microbiol. Mol. Biol. Rev. 62(3):775-806) are now widely used in agriculture and have provided the farmer with an alternative to traditional insect-control methods. However, in some instances these Bt insecticidal proteins may only protect plants from a relatively narrow range of pests. Evolving insect resistance has also presented an issue (Gassmann et al. (2014) PNAS 111(14):5141-6). Thus, novel insect control compositions remain desirable.
BRIEF SUMMARY
[0005] Methods and compositions are provided which employ a silencing element that, when ingested by a plant insect pest, such as Coleopteran, Hemiptera, or Lepidopteran plant pest, including a Diabrotica, Leptinotarsa, Phyllotreta, Acyrthosiphan, Bemisia, Halyomorpha, Nezara, or Spodoptera plant pest, is capable of decreasing the expression of a target sequence in the pest. In certain embodiments, the decrease in expression of the target sequence controls the ability of the pest to reproduce, and thereby the methods and compositions are capable of limiting damage to a plant or the spread of insect pests. Described herein are various target polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or variants or fragments thereof, or complements thereof, that modulate the expression of one or more of the sequences in the target pest RNAs involved in pest reproduction and fecundity. Accordingly, the various target polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or variants or fragments thereof, or complements thereof, are useful in methods described herein to control target pests by insect sterilization and release of sterile target pests, i.e., sterile insect technique ("SIT"). Also provided are silencing elements, which when ingested by the pest, decrease the level of expression of one or more of the target polynucleotides. Further provided are constructs encoding silencing elements and host cells comprising constructs encoding silencing elements. Plants, plant parts, plant cells, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof are also provided. Also provided are formulations of sprayable silencing agents for topical applications to pest insects or substrates where pest insects may be found.
[0006] In another embodiment, a method for controlling a plant insect pest, such as a Coleopteran, Hemiptera, or Lepidopteran plant pest, including a Diabrotica, Leptinotarsa, Phyllotreta, Acyrthosiphan, Bemisia, Halyomorpha, Nezara, or Spodoptera plant pest, is provided. The method comprises feeding to a plant insect pest a composition comprising a silencing element, wherein the silencing element, when ingested by the pest, reduces the level of a target sequence in the pest and thereby controls the pest. Further provided are methods to protect a plant from a plant insect pest. Such methods comprise introducing into the plant or plant part a disclosed silencing element. When the plant expressing the silencing element is ingested by the pest, the level of the target sequence is decreased and the pest is controlled.
BRIEF DESCRIPTION OF THE DRAWINGS
[0007] FIGS. 1A-1D show representative data pertaining to sterilization of adult Western Corn Rootworm ("WCRW") following ingestion of an artificial diet comprising a dsRNA construct comprising a target nucleotide sequence of SEQ ID NO.: 4. FIG. 1A shows the total number of eggs produced within 13-14 days by treatment and age group. For the younger female group, 50 pairs of male and female beetles were used, and for the older female group 50 mated female beetles were used. FIG. 1B shows the average number of eggs produced per female/day during 13-14 day oviposition period by treatment and age group. The box plot graph is produced by Spotfire program indicating 4 quartiles, average, and 95% confidence interval of the mean. FIG. 1C shows the effect of various treatments, as indicated in the figure, on overall average egg hatch rate. Data represents 13-14 days egg collection period; n=6 replication/treatment/day; 5-45 eggs/replication depending on the day (p<0.001). FIG. 1D shows gene suppression analysis in WCRW adult beetles 8 days after treatment of female and male insects for younger age group and 4 days after treatment of female insects for older age group. Relative expression of VgR is shown from 4 individual insects for each treatment using the DV-RPS10 gene as reference and untreated older beetle as normalizer. Box plot shows 4 quartiles, average, median, and 95% confidence interval of the mean by treatment and age group.
[0008] FIGS. 2A-2B show representative data pertaining to sterilization of WCRW following feeding 3.sup.rd instar larvae with an artificial diet comprising a dsRNA construct comprising a target nucleotide sequence of SEQ ID NO.: 4. FIG. 2A shows the average numbers and viable eggs produced per female. Eggs from 15-42 female adult beetles were counted for each treatment. The number in the box shows average numbers of eggs or viable eggs/female. The box plot shows 4 quartiles, average, median, and 95% confidence interval of the mean for each treatment. For the VgR dsRNA exposed group, viable egg production remain very low throughout the study period. Treatment with VgR dsRNA did not affect adult emergence. Mortality of adult beetles due to VgR dsRNA larval exposure was negligible. FIG. 2B shows VgR gene suppression analysis in 4 10-day old beetles and more than 15 28-day old beetles at Days 40 and 58 after treatment, respectively. Box plot of relative expression by qRTPCR shows 4 quartiles, average, median, and 95% confidence interval of the mean for each treatment in 10 and 28 day old beetles. Untreated 3.sup.rd instar larvae were used as normalizer.
[0009] FIGS. 3A-3C show data pertaining to the dose response of WCRW sterilization and gene suppression in WCRW following exposure to an artificial diet comprising a dsRNA comprising a target nucleotide sequence of SEQ ID NO.: 3. FIG. 3A shows the total number of eggs and eggs/female produced during 18 days study period in response to VgR dsRNA doses. Eggs were collected and counted over 18 day oviposition period. Viable eggs/female and net reduction in fecundity (%) are indicated in the last two columns. Net reduction in fecundity (NRF) of VgR dsRNA treated females relative to control (water exposed females) was estimated using the formula described in the Examples. FIG. 3B shows a box plot of percentage of overall egg hatch rates by dose. Data represents 18 days egg collection period; n=1-4 replication/treatment/day; 5-478 eggs/replication depending on the day and availability of eggs. FIG. 3C shows a box plot of relative expression of VgR Day 6 after dsVgR treatment at different doses. Untreated beetles were used as normalizer.
[0010] FIGS. 4A-4B show data pertaining to VgR gene suppression following ingestion of various VgR dsRNA fragments. FIG. 4A shows schematic depiction of the VgR fragments and amplicons of qRTPCR assays (indicated by dashed circles) on VgR coding DNA sequence ("CDS"). FIG. 4B shows a box plot of relative VgR expression 6 days after treatment with dsVgR fragments and controls (ddH2O and dsGUS) using 5'-qRTPCR assay. 4 quartiles, average (horizontal solid line), median (horizontal dash line), and 95% confidence interval of the mean are shown. Similar results were also obtained with Mid- and 3'-qRTPCR assays. Data were normalized to results obtained from untreated 3rd instar larvae.
[0011] FIGS. 5A-5D show data pertaining to VgR fragment screen using gene suppression analysis. FIG. 5A shows a schematic depiction of the VgR fragments used in screen for gene suppression analysis. FIGS. 5B-5D shows representative gene analysis for the indicated VgR fragments using results obtained in three experiments. In each experiment, treatments by water, GUS, and VgR fragment 1 (SEQ ID NO.:3) were included as controls. Data were normalized to beetles treated with water. Two qRTPCR assays (5'- and Mid-qRTPCR assays) were used to avoid overlapping of VgR fragment and PCR amplicon.
[0012] FIGS. 6A-6B show data pertaining to VgR gene suppression in beetles ingesting transgenic plants expressing VgR dsRNA constructs as indicated in the figure. VgR expression in planta is indicated at the bottom of each figure. FIG. 6A shows data in plants at about the V4 growth stage which were infested with at least 14 young female beetles in cages. The plant type is as indicated in the figure, with "NTG" indicating non-transgenic control plants; "Frag1" indicates transgenic plants expressing a silencing element comprising VgR-Frag1 (SEQ ID NO.: 3); "Frag2" indicates transgenic plants expressing a silencing element comprising VgR-Frag2 (SEQ ID NO.: 4), and "Frag3" indicates transgenic plants expressing a silencing element comprising VgR-Frag3 (SEQ ID NO.: 5), Beetles were collected 8 days after feeding for gene suppression analysis. Data were normalized to data from beetles ingesting the NTG control. FIG. 6B shows data obtained from individual R1 maize plants were infested with more than 6 young female beetles in cages. Beetles were collected 12 days after feeding. Each fragment and control is represented by 2 plants used for feeding and more than 12 insects used in gene suppression analysis.
[0013] FIG. 7 shows data pertaining to a fecundity assessment of VgR T1 adult beetle exposure bioassay. For each construct 2-4 events were tested. Each cage received an oviposition dish daily and/or at interval of 2-4 days and eggs were subsequently processed.
[0014] FIG. 8 shows data pertaining to fecundity assessment of VgR T1 larval exposure bioassay. For each event three replicate cages containing at least 8-14 pairs of male and female beetles were arranged. Each cage received oviposition dish every 5 days, and eggs were processed
[0015] FIGS. 9A-9B show data pertaining to WCRW adult sterilization bioassay and gene suppression by DV-BOULE-FRAG1 (SEQ ID NO: 164) dsRNA treatment. FIG. 9A shows the total number of eggs and fertile eggs produced per female; average egg hatch rate with standard error of the mean; reduction in total egg production per female and net reduction in fecundity of female beetles relative to water control. FIG. 9B shows gene expression in beetles after BOULE dsRNA treatment. Relative expression by qRTPCR assay was described in previous examples. The box plot shows four quartiles, average (horizontal dash line), median (horizontal solid line), and 95% confidence interval of the mean are shown.
[0016] FIG. 10 shows data pertaining to beetle counts from larval exposure to BOULE FRAG1 (SEQ ID NO: 164) dsRNA-expressing T1 transgenic plants. The box plot shows four quartiles, average (horizontal dash line), median (horizontal solid line), and 95% confidence interval of the mean. Average expression levels of the BOULE dsRNA fragment in planta for each event were determined in root samples using in vitro transcription (IVT) product as control.
[0017] FIGS. 11A-11C show data pertaining to WCRW larval exposure to BOULE transgenic T1 plants causing adult sterilization. FIG. 11A shows the effect of larval exposure to transgenic plants (expressing DV-BOULE-FRAG1, SEQ ID NO: 164) on the overall average egg production per female and average viable eggs produced per female from emerged beetles. Line in each bar represents the standard error of the mean (.+-.SEM) and the same color bars followed by the same upper or lower case letters are not statistically different. FIG. 11B shows the effect of larval exposure to transgenic plants (expressing DV-BOULE-FRAG1, SEQ ID NO: 164) on hatch rate of eggs obtained from the emerged beetles. The box plot shows four quartiles, average (horizontal white line) and 95% confidence interval of the mean (vertical black line). The average and the corresponding standard error of the means ((.+-.SEM) are indicated at the bottom of the box plot. For each treatment egg hatch test was performed for 5 batches of eggs and a total of at least 1200-1285 eggs per treatment were assessed for viability. FIG. 11C indicates the effect of larval exposure to transgenic plants (expressing DV-BOULE-FRAG1, SEQ ID NO: 164) on net reduction in fecundity of emerged adult beetles relative to NTG control. The box plot shows four quartiles, average (horizontal black line) and 95% confidence interval of the mean (vertical black line). The average and the corresponding standard error of the mean are indicated at the bottom of the box plot.
[0018] FIG. 12 shows data pertaining to 3rd instar sterilization bioassay of dsRNA targeting DV-CUL3-FRAG1, DV-NCLB-FRAG1, and DV-MAEL-FRAG1 dsRNA (SEQ ID No.: 44, 45, and 46 respectively) at 1 ppm. The average total number of eggs produced per female, the average number of viable eggs produced per female, the average egg hatch rate; average reduction in egg production and net reduction in fecundity (both relative to water control) are shown. For each parameter the respective standard error of the mean are presented.
DETAILED DESCRIPTION
[0019] The disclosures herein will be described more fully hereinafter with reference to the accompanying drawings, in which some, but not all possible embodiments are shown. Indeed, disclosures may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein; rather, these embodiments are provided so that this disclosure will satisfy applicable legal requirements.
[0020] Many modifications and other embodiments disclosed herein will come to mind to one skilled in the art to which the disclosed compositions and methods pertain having the benefit of the teachings presented in the foregoing descriptions and the associated drawings. Therefore, it is to be understood that the disclosures are not to be limited to the specific embodiments disclosed and that modifications and other embodiments are intended to be included within the scope of the appended claims. Although specific terms are employed herein, they are used in a generic and descriptive sense only and not for purposes of limitation.
[0021] It is also to be understood that the terminology used herein is for the purpose of describing particular aspects only and is not intended to be limiting. As used in the specification and in the claims, the term "comprising" can include the aspect of "consisting of." Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the disclosed compositions and methods belong. In this specification and in the claims which follow, reference will be made to a number of terms which shall be defined herein.
[0022] As used herein the singular forms "a", "and", and "the" include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to "a cell" includes a plurality of such cells and reference to "the protein" includes reference to one or more proteins and equivalents thereof known to those skilled in the art, and so forth. All technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this invention belongs unless clearly indicated otherwise.
I. Overview
[0023] Methods and compositions are provided which employ one or more silencing elements that, when ingested by a plant insect pest, such as Coleopteran, Hemiptera, or Lepidopteran plant pest, including a Diabrotica, Leptinotarsa, Phyllotreta, Acyrthosiphan, Bemisia, Halyomorpha, Nezara, or Spodoptera plant pest, are capable of decreasing the expression of a target sequence in the pest. In certain embodiments, the decrease in expression of the target sequence controls the ability of the pest to reproduce, and thereby the methods and compositions are capable of limiting damage to a plant or the spread of insect pests. Disclosed herein are target polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or variants and fragments thereof, and complements thereof. Silencing elements comprising sequences, complementary sequences, active fragments or variants of these target polynucleotides are provided which, when ingested by or when contacting the pest, decrease the expression of one or more of the target sequences and thereby controls the pest population via, for example, insect sterilization or through the application of sterile insect technique (SIT; i.e., the silencing elements are associated with sterilization activity). In some embodiments, a transgenic plant comprising a polynucleotide encoding silencing elements are provided which, when ingested by or when contacting the pest, decrease the expression of one or more of the target sequences and thereby controls the pest population via, for example, insect sterilization or through the application of SIT.
[0024] In one embodiment, a method relates to producing sterile insects; releasing sterile insects into the environment in very large numbers (about 10 to 100 times the number of native insects) in order to mate with the native insects that are present in the environment, wherein the native female that mates with a sterile male produce infertile eggs. In a further embodiment, releasing sterile insects is repeated one or more times, wherein the number of native insects decreases and the ratio of sterile to native insects increases, driving the native population size downwards.
[0025] It is understood that target pest RNAs can be involved in one or more of male and/or female sterility, reduction of sperm count, egg production (fecundity), gender ratios, rates of fertilization (fertility), maturation of sexual organs, and sperm or egg viability. SIT has been used to control insect population by mating-based approach through release of sterile insects of one or both genders. In one embodiment, SIT comprises release of large number of sterile male insects that search for and mate with wild females, thereby preventing offspring. SIT using different schemes to generate sterile insects has been reported to control mosquito populations such as Anopheles or Aedes (e.g., see Whyard, et al. (2015) Parasit. Vectors, 8:96; Benedict, M. Q. and A. S. Robinson (2003) Trends Parasitol. 19(8):349; and Nolan, et al. (2011) Genetica 139:33). SIT has been field evaluated for population control of Aedes aegypti in Brazil (Carvalho, D. O. (2015), PLoS. 9(7): e0003864). Dengue, chikungunya, and now Zika virus are all transmitted by Aedes aegypti, one of the most widespread disease-carrying vectors on the globe.
[0026] In one embodiment, a method relates to producing sterile insects; releasing sterile insects into an environment in about 0.5, 1, 5, 10, 20, 30, 50, 60, 70, 90, to 100 times the number of native insects, wherein the sterile insects mate with the native insects that are present in the environment, and wherein the native female that mates with a sterile male produce infertile eggs. In a further embodiment, releasing sterile insects is repeated one or more times, wherein the number of native insects decreases and the ratio of sterile to native insects increases, driving the native population size downwards.
[0027] In one embodiment, compositions and methods are provided which employ a ribonucleic acid construct comprising at least one double-stranded RNA region, at least one strand of which comprises a polynucleotide that is complementary to: (a) a nucleotide sequence comprising a sequence of an RNA transcript expressed in a target pest, wherein the down-regulation of the RNA transcript results in increased sterility in the target; or variants and fragments thereof, and complements of said nucleotide sequence; (b) the nucleotide sequence comprising at least 90% sequence identity to said nucleotide sequence; or variants and fragments thereof, and complements thereof; or (c) the nucleotide sequence comprising at least 19 consecutive nucleotides of said nucleotide sequence; or variants and fragments thereof, and complements thereof; wherein the polynucleotide encodes a silencing element having sterilization activity against an insect plant pest.
[0028] In further embodiment, compositions and methods are provided which employ a ribonucleic acid construct comprising at least one double-stranded RNA region, at least one strand of which comprises a polynucleotide that is complementary to: (a) a nucleotide sequence comprising a sequence of an RNA transcript expressed in a Coleopteran pest, wherein the down-regulation of the RNA transcript results in increased sterility in the target; or variants and fragments thereof, and complements of said nucleotide sequence; (b) the nucleotide sequence comprising at least 90% sequence identity to said nucleotide sequence; or variants and fragments thereof, and complements thereof; or (c) the nucleotide sequence comprising at least 19 consecutive nucleotides of said nucleotide sequence; or variants and fragments thereof, and complements thereof; wherein the polynucleotide encodes a silencing element having sterilization activity against an insect plant pest.
[0029] In another embodiment, compositions and methods are provided which employ a ribonucleic acid construct comprising at least one double-stranded RNA region, at least one strand of which comprises a polynucleotide that is complementary to: (a) the nucleotide sequence comprising any one of SEQ ID NOS: 1-53 or 107-254; or variants and fragments thereof, and complements thereof; (b) the nucleotide sequence comprising at least 90% sequence identity to any one of nucleotides SEQ ID NOS: 1-53 or 107-254; or variants and fragments thereof, and complements thereof; or (c) the nucleotide sequence comprising at least 19 consecutive nucleotides of any one of SEQ ID NOS: 1-53 or 107-254; or variants and fragments thereof, and complements thereof; wherein the polynucleotide encodes a silencing element having sterilization activity against an insect plant pest.
[0030] As used herein, "VgR protein" or "vitellogenin receptor protein" refers to a family of large (180-214 kDa), membrane-bound proteins, and include proteins such as the VgR protein having the sequence of SEQ ID NO.: 106, and variants, homologs, and mutants thereof. It is believed that these proteins bind with high affinity to vitellogenin (K.sub.d values of about 30-180 nM) and are involved in the cellular uptake of vitellogenin. VgR protein is typically expressed in ovarian tissue. As used herein, "BOULE" refers to a family of genes that encode a RNA binding protein with a highly conserved RRM (RNA recognition motif) domain and at least one DAZ (deleted in azoospermia) repeat of 24 amino acids rich in Asn, Tyr, and Gln residues. Deletion or mutations of BOULE in fly usually severely impair spermatogenesis. BOULE is required for meiotic entry and germline differentiation at the transition between G2 and M phases of meiosis. BOULE is typically expressed in germline cells.
[0031] As used herein, "VgR mRNA" or "vitellogenin receptor mRNA" refers to a messenger RNA transcript that when translated provides a VgR protein, or a variant, homolog, or mutant protein thereof.
[0032] As used herein, by "controlling a plant insect pest" or "controls a plant insect pest" is intended any effect on a plant insect pest that results in limiting the damage that the pest causes. Controlling a plant insect pest includes, but is not limited to, killing the pest, inhibiting development of the pest, altering fertility or growth of the pest in such a manner that the pest provides less damage to the plant, or in a manner for decreasing the number of offspring produced, producing less fit pests, including offspring, producing pests more susceptible to predator attack, producing pests more susceptible to other insecticidal proteins, or deterring the pests from eating the plant.
[0033] Reducing the level of expression of the target polynucleotide or the polypeptide encoded thereby, in the pest results in the suppression, control, and/or killing the invading pest. In one embodiment, reducing the level of expression of the target sequence of the pest will reduce the pest damage by at least about 2% to at least about 6%, at least about 5% to about 50%, at least about 10% to about 60%, at least about 30% to about 70%, at least about 40% to about 80%, or at least about 50% to about 90% or greater. Hence, methods disclosed herein can be utilized to control pests, including but not limited to, Coleopteran plant insect pests or a Diabrotica plant pest.
[0034] Certain assays measuring the control of a plant insect pest are commonly known in the art, as are methods to record nodal injury score. See, for example, Oleson et al. (2005) J. Econ. Entomol. 98:1-8. Other assay methods are provided in the examples below.
[0035] Disclosed herein are compositions and methods for protecting plants from a plant insect pest, or inducing resistance in a plant to a plant insect pest, such as Coleopteran plant pests or Diabrotica plant pests or other plant insect pests. Plant insect pests include insects selected from the orders Coleoptera, Diptera, Hymenoptera, Lepidoptera, Mallophaga, Homoptera, Hemiptera Orthroptera, Thysanoptera, Dermaptera, Isoptera, Anoplura, Siphonaptera, Trichoptera, etc., particularly Lepidoptera and Coleoptera.
[0036] Those skilled in the art will recognize that not all compositions are equally effective against all pests. Disclosed compositions, including the silencing elements disclosed herein, display activity against plant insect pests, which may include economically important agronomic, forest, greenhouse, nursery ornamentals, food and fiber, public and animal health, domestic and commercial structure, household and stored product pests.
[0037] As used herein "Coleopteran plant pest" is used to refer to any member of the Coleoptera order. Other plant insect pests that may be targeted by the methods and compositions disclosed herein, but are not limited to Mexican Bean Beetle (Epilachna varivestis), and Colorado potato beetle (Leptinotarsa decemlineata).
[0038] As used herein, the term "Diabrotica plant pest" is used to refer to any member of the Diabrotica genus. Accordingly, the compositions and methods are also useful in protecting plants against any Diabrotica plant pest including, for example, Diabrotica adelpha; Diabrotica amecameca; Diabrotica balteata; Diabrotica barberi; Diabrotica biannularis; Diabrotica cristata; Diabrotica decempunctata; Diabrotica dissimilis; Diabrotica lemniscata; Diabrotica limitata (including, for example, Diabrotica limitata quindecimpuncata); Diabrotica longicornis; Diabrotica nummularis; Diabrotica porracea; Diabrotica scutellata; Diabrotica sexmaculata; Diabrotica speciosa (including, for example, Diabrotica speciosa speciosa); Diabrotica tibialis; Diabrotica undecimpunctata (including, for example, Southern corn rootworm (Diabrotica undecimpunctata), Diabrotica undecimpunctata duodecimnotata; Diabrotica undecimpunctata howardi (spotted cucumber beetle); Diabrotica undecimpunctata undecimpunctata (western spotted cucumber beetle)); Diabrotica virgifera (including, for example, Diabrotica virgifera virgifera (western corn rootworm) and Diabrotica virgifera zeae (Mexican corn rootworm)); Diabrotica viridula; Diabrotica wartensis; Diabrotica sp. JJG335; Diabrotica sp. JJG336; Diabrotica sp. JJG341; Diabrotica sp. JJG356; Diabrotica sp. JJG362; and, Diabrotica sp. JJG365.
[0039] In certain embodiments, the Diabrotica plant pest comprises D. virgifera virgifera, D. barberi, D. virgifera zeae, D. speciosa, or D. undecimpunctata howardi.
[0040] Larvae of the order Lepidoptera include, but are not limited to, armyworms, cutworms, loopers and heliothines in the family Noctuidae Spodoptera frugiperda JE Smith (fall armyworm); S. exigua Hubner (beet armyworm); S. litura Fabricius (tobacco cutworm, cluster caterpillar); Mamestra configurata Walker (bertha armyworm); M. brassicae Linnaeus (cabbage moth); Agrotis ipsilon Hufnagel (black cutworm); A. orthogonia Morrison (western cutworm); A. subterranea Fabricius (granulate cutworm); Alabama argillacea Hubner (cotton leaf worm); Trichoplusia ni Hubner (cabbage looper); Pseudoplusia includens Walker (soybean looper); Anticarsia gemmatalis Hubner (velvetbean caterpillar); Hypena scabra Fabricius (green cloverworm); Heliothis virescens Fabricius (tobacco budworm); Pseudaletia unipuncta Haworth (armyworm); Athetis mindara Barnes and Mcdunnough (rough skinned cutworm); Euxoa messoria Harris (darksided cutworm); Earias insulana Boisduval (spiny bollworm); E. vittella Fabricius (spotted bollworm); Helicoverpa armigera Hubner (American bollworm); H. zea Boddie (corn earworm or cotton bollworm); Melanchra picta Harris (zebra caterpillar); Egira (Xylomyges) curialis Grote (citrus cutworm); borers, casebearers, webworms, coneworms, and skeletonizers from the family Pyralidae Ostrinia nubilalis Hubner (European corn borer); Amyelois transitella Walker (naval orangeworm); Anagasta kuehniella Zeller (Mediterranean flour moth); Cadra cautella Walker (almond moth); Chilo suppressalis Walker (rice stem borer); C. partellus, (sorghum borer); Corcyra cephalonica Stainton (rice moth); Crambus caliginosellus Clemens (corn root webworm); C. teterrellus Zincken (bluegrass webworm); Cnaphalocrocis medinalis Guenee (rice leaf roller); Desmia funeralis Hubner (grape leaffolder); Diaphania hyalinata Linnaeus (melon worm); D. nitidalis Stoll (pickleworm); Diatraea grandiosella Dyar (southwestern corn borer), D. saccharalis Fabricius (surgarcane borer); Eoreuma loftini Dyar (Mexican rice borer); Ephestia elutella Hubner (tobacco (cacao) moth); Galleria mellonella Linnaeus (greater wax moth); Herpetogramma licarsisalis Walker (sod webworm); Homoeosoma electellum Hulst (sunflower moth); Elasmopalpus lignosellus Zeller (lesser cornstalk borer); Achroia grisella Fabricius (lesser wax moth); Loxostege sticticalis Linnaeus (beet webworm); Orthaga thyrisalis Walker (tea tree web moth); Maruca testulalis Geyer (bean pod borer); Plodia interpunctella Hubner (Indian meal moth); Scirpophaga incertulas Walker (yellow stem borer); Udea rubigalis Guenee (celery leaftier); and leafrollers, budworms, seed worms and fruit worms in the family Tortricidae Acleris gloverana Walsingham (Western blackheaded budworm); A. variana Fernald (Eastern blackheaded budworm); Archips argyrospila Walker (fruit tree leaf roller); A. rosana Linnaeus (European leaf roller); and other Archips species, Adoxophyes orana Fischer von Rosslerstamm (summer fruit tortrix moth); Cochylis hospes Walsingham (banded sunflower moth); Cydia latiferreana Walsingham (filbertworm); C. pomonella Linnaeus (coding moth); Platynota flavedana Clemens (variegated leafroller); P. stultana Walsingham (omnivorous leafroller); Lobesia botrana Denis & Schiffermullier (European grape vine moth); Spilonota ocellana Denis & Schiffermuller (eyespotted bud moth); Endopiza viteana Clemens (grape berry moth); Eupoecilia ambiguella Hubner (vine moth); Bonagota salubricola Meyrick (Brazilian apple leafroller); Grapholita molesta Busck (oriental fruit moth); Suleima helianthana Riley (sunflower bud moth); Argyrotaenia spp.; Choristoneura spp.
[0041] Selected other agronomic pests in the order Lepidoptera include, but are not limited to, Alsophila pometaria Harris (fall cankerworm); Anarsia lineatella Zeller (peach twig borer); Anisota senatoria J. E. Smith (orange striped oakworm); Antheraea pernyi Guerin-Meneville (Chinese Oak Tussah Moth); Bombyx mori Linnaeus (Silkworm); Bucculatrix thurberiella Busck (cotton leaf perforator); Colias eurytheme Boisduval (alfalfa caterpillar); Datana integerrima Grote & Robinson (walnut caterpillar); Dendrolimus sibiricus Tschetwerikov (Siberian silk moth), Ennomos subsignaria Hubner (elm spanworm); Erannis tiliaria Harris (linden looper); Euproctis chrysorrhoea Linnaeus (browntail moth); Harrisina americana Guerin-Meneville (grapeleaf skeletonizer); Hemileuca oliviae Cockrell (range caterpillar); Hyphantria cunea Drury (fall webworm); Keiferia lycopersicella Walsingham (tomato pinworm); Lambdina fiscellaria fiscellaria Hulst (Eastern hemlock looper); L. fiscellaria lugubrosa Hulst (Western hemlock looper); Leucoma salicis Linnaeus (satin moth); Lymantria dispar Linnaeus (gypsy moth); Manduca quinquemaculata Haworth (five spotted hawk moth, tomato hornworm); M. sexta Haworth (tomato hornworm, tobacco hornworm); Operophtera brumata Linnaeus (winter moth); Paleacrita vernata Peck (spring cankerworm); Papilio cresphontes Cramer (giant swallowtail orange dog); Phryganidia californica Packard (California oakworm); Phyllocnistis citrella Stainton (citrus leafminer); Phyllonorycter blancardella Fabricius (spotted tentiform leafminer); Pieris brassicae Linnaeus (large white butterfly); P. rapae Linnaeus (small white butterfly); P. napi Linnaeus (green veined white butterfly); Platyptilia carduidactyla Riley (artichoke plume moth); Plutella xylostella Linnaeus (diamondback moth); Pectinophora gossypiella Saunders (pink bollworm); Pontia protodice Boisduval and Leconte (Southern cabbageworm); Sabulodes aegrotata Guenee (omnivorous looper); Schizura concinna J. E. Smith (red humped caterpillar); Sitotroga cerealella Olivier (Angoumois grain moth); Thaumetopoea pityocampa Schiffermuller (pine processionary caterpillar); Tineola bisselliella Hummel (webbing clothesmoth); Tuta absoluta Meyrick (tomato leafminer); Yponomeuta padella Linnaeus (ermine moth); Heliothis subflexa Guenee; Malacosoma spp. and Orgyia spp.
[0042] Of interest are larvae and adults of the order Coleoptera including weevils from the families Anthribidae, Bruchidae and Curculionidae (including, but not limited to: Anthonomus grandis Boheman (boll weevil); Lissorhoptrus oryzophilus Kuschel (rice water weevil); Sitophilus granarius Linnaeus (granary weevil); S. oryzae Linnaeus (rice weevil); Hypera punctata Fabricius (clover leaf weevil); Cylindrocopturus adspersus LeConte (sunflower stem weevil); Smicronyx fulvus LeConte (red sunflower seed weevil); S. sordidus LeConte (gray sunflower seed weevil); Sphenophorus maidis Chittenden (maize billbug)); flea beetles, cucumber beetles, rootworms, leaf beetles, potato beetles and leafminers in the family Chrysomelidae (including, but not limited to: Leptinotarsa decemlineata Say (Colorado potato beetle); Diabrotica virgifera virgifera LeConte (western corn rootworm); D. barberi Smith and Lawrence (northern corn rootworm); D. undecimpunctata howardi Barber (southern corn rootworm); Chaetocnema pulicaria Melsheimer (corn flea beetle); Phyllotreta cruciferae Goeze (Crucifer flea beetle); Phyllotreta striolata (stripped flea beetle); Colaspis brunnea Fabricius (grape colaspis); Oulema melanopus Linnaeus (cereal leaf beetle); Zygogramma exclamationis Fabricius (sunflower beetle)); beetles from the family Coccinellidae (including, but not limited to: Epilachna varivestis Mulsant (Mexican bean beetle)); chafers and other beetles from the family Scarabaeidae (including, but not limited to: Popillia japonica Newman (Japanese beetle); Cyclocephala borealis Arrow (northern masked chafer, white grub); C. immaculata Olivier (southern masked chafer, white grub); Rhizotrogus majalis Razoumowsky (European chafer); Phyllophaga crinita Burmeister (white grub); Ligyrus gibbosus De Geer (carrot beetle)); carpet beetles from the family Dermestidae; wireworms from the family Elateridae, Eleodes spp., Melanotus spp.; Conoderus spp.; Limonius spp.; Agriotes spp.; Ctenicera spp.; Aeolus spp.; bark beetles from the family Scolytidae and beetles from the family Tenebrionidae.
[0043] Adults and immatures of the order Diptera are of interest, including leafminers Agromyza parvicornis Loew (corn blotch leafminer); midges (including, but not limited to: Contarinia sorghicola Coquillett (sorghum midge); Mayetiola destructor Say (Hessian fly); Sitodiplosis mosellana Gain (wheat midge); Neolasioptera murtfeldtiana Felt, (sunflower seed midge)); fruit flies (Tephritidae), Oscinella frit Linnaeus (fruit flies); maggots (including, but not limited to: Delia platura Meigen (seedcorn maggot); D. coarctate Fallen (wheat bulb fly) and other Delia spp., Meromyza americana Fitch (wheat stem maggot); Musca domestica Linnaeus (house flies); Fannia canicularis Linnaeus, F. femoralis Stein (lesser house flies); Stomoxys calcitrans Linnaeus (stable flies)); face flies, horn flies, blow flies, Chrysomya spp.; Phormia spp. and other muscoid fly pests, horse flies Tabanus spp.; bot flies Gastrophilus spp.; Oestrus spp.; cattle grubs Hypoderma spp.; deer flies Chrysops spp.; Melophagus ovinus Linnaeus (keds) and other Brachycera, mosquitoes Aedes spp.; Anopheles spp.; Culex spp.; black flies Prosimulium spp.; Simulium spp.; biting midges, sand flies, sciarids, and other Nematocera.
[0044] Included as insects of interest are adults and nymphs of the orders Hemiptera and Homoptera such as, but not limited to, adelgids from the family Adelgidae, plant bugs from the family Miridae, cicadas from the family Cicadidae, leafhoppers, Empoasca spp.; from the family Cicadellidae, planthoppers from the families Cixiidae, Flatidae, Fulgoroidea, Issidae and Delphacidae, treehoppers from the family Membracidae, psyllids from the family Psyllidae, whiteflies from the family
[0045] Aleyrodidae, aphids from the family Aphididae, phylloxera from the family Phylloxeridae, mealybugs from the family Pseudococcidae, scales from the families Asterolecanidae, Coccidae, Dactylopiidae, Diaspididae, Eriococcidae Ortheziidae, Phoenicococcidae and Margarodidae, lace bugs from the family Tingidae, stink bugs from the family Pentatomidae, cinch bugs, Blissus spp.; and other seed bugs from the family Lygaeidae, spittlebugs from the family Cercopidae squash bugs from the family Coreidae and red bugs and cotton stainers from the family Pyrrhocoridae.
[0046] Agronomically important members from the order Homoptera further include, but are not limited to: Acyrthisiphon pisum Harris (pea aphid); Aphis craccivora Koch (cowpea aphid); A. fabae Scopoli (black bean aphid); A. gossypii Glover (cotton aphid, melon aphid); A. maidiradicis Forbes (corn root aphid); A. pomi De Geer (apple aphid); A. spiraecola Patch (spirea aphid); Aulacorthum solani Kaltenbach (foxglove aphid); Chaetosiphon fragaefolii Cockerell (strawberry aphid); Diuraphis noxia Kurdjumov/Mordvilko (Russian wheat aphid); Dysaphis plantaginea Paaserini (rosy apple aphid); Eriosoma lanigerum Hausmann (woolly apple aphid); Brevicoryne brassicae Linnaeus (cabbage aphid); Hyalopterus pruni Geoffroy (mealy plum aphid); Lipaphis erysimi Kaltenbach (turnip aphid); Metopolophium dirrhodum Walker (cereal aphid); Macrosiphum euphorbiae Thomas (potato aphid); Myzus persicae Sulzer (peach-potato aphid, green peach aphid); Nasonovia ribisnigri Mosley (lettuce aphid); Pemphigus spp. (root aphids and gall aphids); Rhopalosiphum maidis Fitch (corn leaf aphid); R. padi Linnaeus (bird cherry-oat aphid); Schizaphis graminum Rondani (greenbug); Sipha flava Forbes (yellow sugarcane aphid); Sitobion avenae Fabricius (English grain aphid); Therioaphis maculata Buckton (spotted alfalfa aphid); Toxoptera aurantii Boyer de Fonscolombe (black citrus aphid) and T. citricida Kirkaldy (brown citrus aphid); Adelges spp. (adelgids); Phylloxera devastatrix Pergande (pecan phylloxera); Bemisia tabaci Gennadius (tobacco whitefly, sweetpotato whitefly); B. argentifolii Bellows & Perring (silverleaf whitefly); Dialeurodes citri Ashmead (citrus whitefly); Trialeurodes abutiloneus (bandedwinged whitefly) and T. vaporariorum Westwood (greenhouse whitefly); Empoasca fabae Harris (potato leafhopper); Laodelphax striatellus Fallen (smaller brown planthopper); Macrolestes quadrilineatus Forbes (aster leafhopper); Nephotettix cinticeps Uhler (green leafhopper); N. nigropictus Stal (rice leafhopper); Nilaparvata lugens Stal (brown planthopper); Peregrinus maidis Ashmead (corn planthopper); Sogatella furcifera Horvath (white-backed planthopper); Sogatodes orizicola Muir (rice delphacid); Typhlocyba pomaria McAtee (white apple leafhopper); Erythroneoura spp. (grape leafhoppers); Magicicada septendecim Linnaeus (periodical cicada); Icerya purchasi Maskell (cottony cushion scale); Quadraspidiotus perniciosus Comstock (San Jose scale); Planococcus citri Risso (citrus mealybug); Pseudococcus spp. (other mealybug complex); Cacopsylla pyricola Foerster (pear psylla); Trioza diospyri Ashmead (persimmon psylla).
[0047] Agronomically important species of interest from the order Hemiptera include, but are not limited to: Acrosternum hilare Say (green stink bug); Anasa tristis De Geer (squash bug); Blissus leucopterus leucopterus Say (chinch bug); Corythuca gossypii Fabricius (cotton lace bug); Cyrtopeltis modesta Distant (tomato bug); Dysdercus suturellus Herrich-Schiffer (cotton stainer); Euschistus servus Say (brown stink bug); E. variolarius Palisot de Beauvois (one-spotted stink bug); Graptostethus spp. (complex of seed bugs); Leptoglossus corculus Say (leaf-footed pine seed bug); Lygus lineolaris Palisot de Beauvois (tarnished plant bug); L. Hesperus Knight (Western tarnished plant bug); L. pratensis Linnaeus (common meadow bug); L. rugulipennis Poppius (European tarnished plant bug); Lygocoris pabulinus Linnaeus (common green capsid); Nezara viridula Linnaeus (southern green stink bug); Oebalus pugnax Fabricius (rice stink bug); Oncopeltus fasciatus Dallas (large milkweed bug); Pseudatomoscelis seriatus Reuter (cotton fleahopper).
[0048] Furthermore, embodiments may be effective against Hemiptera such, Calocoris norvegicus Gmelin (strawberry bug); Orthops campestris Linnaeus; Plesiocoris rugicollis Fallen (apple capsid); Cyrtopeltis modestus Distant (tomato bug); Cyrtopeltis notatus Distant (suckfly); Spanagonicus albofasciatus Reuter (whitemarked fleahopper); Diaphnocoris chlorionis Say (honeylocust plant bug); Labopidicola allii Knight (onion plant bug); Pseudatomoscelis seriatus Reuter (cotton fleahopper); Adelphocoris rapidus Say (rapid plant bug); Poecilocapsus lineatus Fabricius (four-lined plant bug); Nysius ericae Schilling (false chinch bug); Nysius raphanus Howard (false chinch bug); Nezara viridula Linnaeus (Southern green stink bug); Eurygaster spp.; Coreidae spp.; Pyrrhocoridae spp.; Tinidae spp.; Blostomatidae spp.; Reduviidae spp. and Cimicidae spp.
[0049] Also included are adults and larvae of the order Acari (mites) such as Aceria tosichella Keifer (wheat curl mite); Petrobia latens Muller (brown wheat mite); spider mites and red mites in the family Tetranychidae, Panonychus ulmi Koch (European red mite); Tetranychus urticae Koch (two spotted spider mite); (T. mcdanieli McGregor (McDaniel mite); T. cinnabarinus Boisduval (carmine spider mite); T. turkestani Ugarov & Nikolski (strawberry spider mite); flat mites in the family Tenuipalpidae, Brevipalpus lewisi McGregor (citrus flat mite); rust and bud mites in the family Eriophyidae and other foliar feeding mites and mites important in human and animal health, i.e., dust mites in the family Epidermoptidae, follicle mites in the family Demodicidae, grain mites in the family Glycyphagidae, ticks in the order Ixodidae. Ixodes scapularis Say (deer tick); I. holocyclus Neumann (Australian paralysis tick); Dermacentor variabilis Say (American dog tick); Amblyomma americanum Linnaeus (lone star tick) and scab and itch mites in the families Psoroptidae, Pyemotidae and Sarcoptidae.
[0050] Insect pests of the order Thysanura are of interest, such as Lepisma saccharina Linnaeus (silverfish); Thermobia domestica Packard (firebrat).
[0051] Insect pests of interest include the superfamily of stink bugs and other related insects including but not limited to species belonging to the family Pentatomidae (Nezara viridula, Halyomorpha halys, Piezodorus guildini, Euschistus servus, Acrosternum hilare, Euschistus heros, Euschistus tristigmus, Acrosternum hilare, Dichelops furcatus, Dichelops melacanthus, and Bagrada hilaris (Bagrada Bug)), the family Plataspidae (Megacopta cribraria--Bean plataspid) and the family Cydnidae (Scaptocoris castanea--Root stink bug) and Lepidoptera species including but not limited to: diamond-back moth, e.g., Helicoverpa zea Boddie; soybean looper, e.g., Pseudoplusia includens Walker and velvet bean caterpillar e.g., Anticarsia gemmatalis Hubner.
II. Target Sequences
[0052] As used herein, a "target sequence" or "target polynucleotide" comprises any sequence in the pest that one desires to reduce the level of expression thereof. In certain embodiments, decreasing the level of expression of the target sequence in the pest controls the pest. For instance, the target sequence may be essential for growth and development. Non-limiting examples of target sequences include a polynucleotide set forth in SEQ ID NOS.: 1-53 or 107-254, or variants and fragments thereof, and complements thereof. As exemplified elsewhere herein, decreasing the level of expression of one or more of these target sequences in a Coleopteran plant pest or a Diabrotica plant pest controls the pest.
III. Silencing Elements
[0053] By "silencing element" is intended a polynucleotide which when contacted by or ingested by a plant insect pest, is capable of reducing or eliminating the level or expression of a target polynucleotide or the polypeptide encoded thereby. Accordingly, it is to be understood that "silencing element," as used herein, comprises polynucleotides such as RNA constructs, double stranded RNA (dsRNA), hairpin RNA, and sense and/or antisense RNA. In one embodiment, the silencing element employed can reduce or eliminate the expression level of the target sequence by influencing the level of the target RNA transcript or, alternatively, by influencing translation and thereby affecting the level of the encoded polypeptide. Methods to assay for functional silencing elements that are capable of reducing or eliminating the level of a sequence of interest are disclosed elsewhere herein. A single polynucleotide employed in the disclosed methods can comprise one or more silencing elements to the same or different target polynucleotides. The silencing element can be produced in vivo (i.e., in a host cell such as a plant or microorganism) or in vitro.
[0054] In certain embodiments, a silencing element may comprise a chimeric construction molecule comprising two or more disclosed sequences or portions thereof. For example, the chimeric construction may be a hairpin or dsRNA as disclosed herein. A chimera may comprise two or more disclosed sequences or portions thereof. In one embodiment, a chimera contemplates two complementary sequences set forth herein, or portions thereof, having some degree of mismatch between the complementary sequences such that the two sequences are not perfect complements of one another. Providing at least two different sequences in a single silencing element may allow for targeting multiple genes using one silencing element and/or for example, one expression cassette. Targeting multiple genes may allow for slowing or reducing the possibility of resistance by the pest. In addition, providing multiple targeting ability in one expressed molecule may reduce the expression burden of the transformed plant or plant product, or provide topical treatments that are capable of targeting multiple hosts with one application.
[0055] In certain embodiments, while the silencing element controls pests, preferably the silencing element has no effect on the normal plant or plant part.
[0056] As discussed in further detail below, silencing elements can include, but are not limited to, a sense suppression element, an antisense suppression element, a double stranded RNA, a siRNA, an amiRNA, a miRNA, or a hairpin suppression element. In an embodiment, silencing elements may comprise a chimera where two or more disclosed sequences or active fragments or variants, or complements thereof, are found in the same RNA molecule. In various embodiments, a disclosed sequence or active fragment or variant, or complement thereof, may be present as more than one copy in a DNA construct, silencing element, DNA molecule or RNA molecule. In a hairpin or dsRNA molecule, the location of a sense or antisense sequence in the molecule, for example, in which sequence is transcribed first or is located on a particular terminus of the RNA molecule, is not limiting to the disclosed sequences, and the dsRNA is not to be limited by disclosures herein of a particular location for such a sequence. Non-limiting examples of silencing elements that can be employed to decrease expression of these target sequences comprise fragments or variants of the sense or antisense sequence, or alternatively consists of the sense or antisense sequence, of a sequence set forth in SEQ ID NOS.: 1-53 or 107-254, or variants and fragments thereof, and complements thereof. The silencing element can further comprise additional sequences that advantageously effect transcription and/or the stability of a resulting transcript. For example, the silencing elements can comprise at least one thymine residue at the 3' end. This can aid in stabilization. Thus, the silencing elements can have at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more thymine residues at the 3' end. As discussed in further detail below, enhancer suppressor elements can also be employed in conjunction with the silencing elements disclosed herein.
[0057] By "reduces" or "reducing" the expression level of a polynucleotide or a polypeptide encoded thereby is intended to mean, the polynucleotide or polypeptide level of the target sequence is statistically lower than the polynucleotide level or polypeptide level of the same target sequence in an appropriate control pest which is not exposed to (i.e., has not ingested or come into contact with) the silencing element. In particular embodiments, methods and/or compositions disclosed herein reduce the polynucleotide level and/or the polypeptide level of the target sequence in a plant insect pest to less than 95%, less than 90%, less than 80%, less than 70%, less than 60%, less than 50%, less than 40%, less than 30%, less than 20%, less than 10%, or less than 5% of the polynucleotide level, or the level of the polypeptide encoded thereby, of the same target sequence in an appropriate control pest. In some embodiments, a silencing element has substantial sequence identity to the target polynucleotide, typically greater than about 65% sequence identity, greater than about 85% sequence identity, about 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity. Furthermore, a silencing element can be complementary to a portion of the target polynucleotide. Generally, sequences of at least 15, 16, 17, 18, 19, 20, 22, 25, 50, 100, 200, 300, 400, 450 continuous nucleotides or greater of the sequence set forth in any of SEQ ID NOS.: 1-53 or 107-254, or variants and fragments thereof, and complements thereof may be used. Methods to assay for the level of the RNA transcript, the level of the encoded polypeptide, or the activity of the polynucleotide or polypeptide are discussed elsewhere herein.
[0058] i. Sense Suppression Elements
[0059] As used herein, a "sense suppression element" comprises a polynucleotide designed to express an RNA molecule corresponding to at least a part of a target messenger RNA in the "sense" orientation. Expression of the RNA molecule comprising the sense suppression element reduces or eliminates the level of the target polynucleotide or the polypeptide encoded thereby. The polynucleotide comprising the sense suppression element may correspond to all or part of the sequence of the target polynucleotide, all or part of the 5' and/or 3' untranslated region of the target polynucleotide, all or part of the coding sequence of the target polynucleotide, or all or part of both the coding sequence and the untranslated regions of the target polynucleotide.
[0060] Typically, a sense suppression element has substantial sequence identity to the target polynucleotide, typically greater than about 65% sequence identity, greater than about 85% sequence identity, about 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity. See, U.S. Pat. Nos. 5,283,184 and 5,034,323; herein incorporated by reference. The sense suppression element can be any length so long as it allows for the suppression of the targeted sequence. The sense suppression element can be, for example, 15, 16, 17, 18, 19, 20, 22, 25, 30, 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 600, 700, 900, 1000, 1100, 1200, 1300 nucleotides or longer of the target polynucleotides set forth in any of SEQ ID NOS.: 1-53 or 107-254, or variants and fragments thereof, and complements thereof. In other embodiments, the sense suppression element can be, for example, about 15-25, 19-35, 19-50, 25-100, 100-150, 150-200, 200-250, 250-300, 300-350, 350-400, 450-500, 500-550, 550-600, 600-650, 650-700, 700-750, 750-800, 800-850, 850-900, 900-950, 950-1000, 1000-1050, 1050-1100, 1100-1200, 1200-1300, 1300-1400, 1400-1500, 1500-1600, 1600-1700, 1700-1800 nucleotides or longer of the target polynucleotides set forth in any of SEQ ID NOS.: 1-53 or 107-254, or variants and fragments thereof, and complements thereof.
[0061] ii. Antisense Suppression Elements
[0062] As used herein, an "antisense suppression element" comprises a polynucleotide which is designed to express an RNA molecule complementary to all or part of a target messenger RNA. Expression of the antisense RNA suppression element reduces or eliminates the level of the target polynucleotide. The polynucleotide for use in antisense suppression may correspond to all or part of the complement of the sequence encoding the target polynucleotide, all or part of the complement of the 5' and/or 3' untranslated region of the target polynucleotide, all or part of the complement of the coding sequence of the target polynucleotide, or all or part of the complement of both the coding sequence and the untranslated regions of the target polynucleotide. In addition, the antisense suppression element may be fully complementary (i.e., 100% identical to the complement of the target sequence) or partially complementary (i.e., less than 100% identical to the complement of the target sequence) to the target polynucleotide. In certain embodiments, the antisense suppression element comprises at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence complementarity to the target polynucleotide. Antisense suppression may be used to inhibit the expression of multiple proteins in the same plant. See, for example, U.S. Pat. No. 5,942,657. Furthermore, the antisense suppression element can be complementary to a portion of the target polynucleotide. Generally, sequences of at least 15, 16, 17, 18, 19, 20, 22, 25, 50, 100, 200, 300, 400, 450 nucleotides or greater of the sequence set forth in any of SEQ ID NOS.: 1-53 or 107-254, or variants and fragments thereof, and complements thereof may be used. Methods for using antisense suppression to inhibit the expression of endogenous genes in plants are described, for example, in Liu et al (2002) Plant Physiol. 129:1732-1743 and U.S. Pat. No. 5,942,657, which is herein incorporated by reference.
[0063] iii. Double Stranded RNA Suppression Element
[0064] A "double stranded RNA silencing element" or "dsRNA," comprises at least one transcript that is capable of forming a dsRNA either before or after ingestion by a plant insect pest. Thus, a "dsRNA silencing element" includes a dsRNA, a transcript or polyribonucleotide capable of forming a dsRNA or more than one transcript or polyribonucleotide capable of forming a dsRNA. "Double stranded RNA" or "dsRNA" refers to a polyribonucleotide structure formed either by a single self-complementary RNA molecule or a polyribonucleotide structure formed by the expression of at least two distinct RNA strands. The dsRNA molecule(s) employed in the disclosed methods and compositions mediate the reduction of expression of a target sequence, for example, by mediating RNA interference "RNAi" or gene silencing in a sequence-specific manner In various embodiments, the dsRNA is capable of reducing or eliminating the level or expression of a target polynucleotide or the polypeptide encoded thereby in a plant insect pest.
[0065] The dsRNA can reduce or eliminate the expression level of the target sequence by influencing the level of the target RNA transcript, by influencing translation and thereby affecting the level of the encoded polypeptide, or by influencing expression at the pre-transcriptional level (i.e., via the modulation of chromatin structure, methylation pattern, etc., to alter gene expression). For example, see Verdel et al. (2004) Science 303:672-676; Pal-Bhadra et al. (2004) Science 303:669-672; Allshire (2002) Science 297:1818-1819; Volpe et al. (2002) Science 297:1833-1837; Jenuwein (2002) Science 297:2215-2218; and Hall et al. (2002) Science 297:2232-2237. Methods to assay for functional dsRNA that are capable of reducing or eliminating the level of a sequence of interest are disclosed elsewhere herein. Accordingly, as used herein, the term "dsRNA" is meant to encompass other terms used to describe nucleic acid molecules that are capable of mediating RNA interference or gene silencing, including, for example, short-interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), hairpin RNA, short hairpin RNA (shRNA), post-transcriptional gene silencing RNA (ptgsRNA), and others.
[0066] In certain embodiments, at least one strand of the duplex or double-stranded region of the dsRNA shares sufficient sequence identity or sequence complementarity to the target polynucleotide to allow the dsRNA to reduce the level of expression of the target sequence. In some embodiments, a dsRNA has substantial sequence identity to the target polynucleotide, typically greater than about 65% sequence identity, greater than about 85% sequence identity, about 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity. Furthermore, a dsRNA element can be complementary to a portion of the target polynucleotide. Generally, sequences of at least 15, 16, 17, 18, 19, 20, 22, 25, 50, 100, 200, 300, 400, 450 nucleotides or greater of the sequence set forth in any of SEQ ID NOS.: 1-53 or 107-254, or variants and fragments thereof, and complements thereof may be used. As used herein, the strand that is complementary to the target polynucleotide is the "antisense strand" and the strand homologous to the target polynucleotide is the "sense strand."
[0067] In another embodiment, the dsRNA comprises a hairpin RNA. A hairpin RNA comprises an RNA molecule that is capable of folding back onto itself to form a double stranded structure. Multiple structures can be employed as hairpin elements. In certain embodiments, the dsRNA suppression element comprises a hairpin element which comprises in the following order, a first segment, a second segment, and a third segment, where the first and the third segment share sufficient complementarity to allow the transcribed RNA to form a double-stranded stem-loop structure.
[0068] The "second segment" of the hairpin comprises a "loop" or a "loop region." These terms are used synonymously herein and are to be construed broadly to comprise any nucleotide sequence that confers enough flexibility to allow self-pairing to occur between complementary regions of a polynucleotide (i.e., segments 1 and 3 which form the stem of the hairpin). For example, in some embodiments, the loop region may be substantially single stranded and act as a spacer between the self-complementary regions of the hairpin stem-loop. In some embodiments, the loop region can comprise a random or nonsense nucleotide sequence and thus not share sequence identity to a target polynucleotide. In other embodiments, the loop region comprises a sense or an antisense RNA sequence or fragment thereof that shares identity to a target polynucleotide. See, for example, International Patent Publication No. WO 02/00904. In certain embodiments, the loop sequence can include an intron sequence, a sequence derived from an intron sequence, a sequence homologous to an intron sequence, or a modified intron sequence. The intron sequence can be one found in the same or a different species from which segments 1 and 3 are derived. In certain embodiments, the loop region can be optimized to be as short as possible while still providing enough intramolecular flexibility to allow the formation of the base-paired stem region. Accordingly, the loop sequence is generally less than 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100, 50, 25, 20, 19, 18, 17, 16, 15, 10 nucleotides or less.
[0069] The "first" and the "third" segment of the hairpin RNA molecule comprise the base-paired stem of the hairpin structure. The first and the third segments are inverted repeats of one another and share sufficient complementarity to allow the formation of the base-paired stem region. In certain embodiments, the first and the third segments are fully complementary to one another. Alternatively, the first and the third segment may be partially complementary to each other so long as they are capable of hybridizing to one another to form a base-paired stem region. The amount of complementarity between the first and the third segment can be calculated as a percentage of the entire segment. Thus, the first and the third segment of the hairpin RNA generally share at least 50%, 60%, 70%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, up to and including 100% complementarity.
[0070] The first and the third segment are at least about 1000, 500, 475, 450, 425, 400, 375, 350, 325, 300, 250, 225, 200, 175, 150, 125, 100, 75, 60, 50, 40, 30, 25, 22, 20, 19, 18, 17, 16, 15 or 10 nucleotides in length. In certain embodiments, the length of the first and/or the third segment is about 10-100 nucleotides, about 10 to about 75 nucleotides, about 10 to about 50 nucleotides, about 10 to about 40 nucleotides, about 10 to about 35 nucleotides, about 10 to about 30 nucleotides, about 10 to about 25 nucleotides, about 10 to about 19 nucleotides, about 10 to about 20 nucleotides, about 19 to about 50 nucleotides, about 50 nucleotides to about 100 nucleotides, about 100 nucleotides to about 150 nucleotides, about 100 nucleotides to about 300 nucleotides, about 150 nucleotides to about 200 nucleotides, about 200 nucleotides to about 250 nucleotides, about 250 nucleotides to about 300 nucleotides, about 300 nucleotides to about 350 nucleotides, about 350 nucleotides to about 400 nucleotides, about 400 nucleotide to about 500 nucleotides, about 600 nt, about 700 nt, about 800 nt, about 900 nt, about 1000 nt, about 1100 nt, about 1200 nt, 1300 nt, 1400 nt, 1500 nt, 1600 nt, 1700 nt, 1800 nt, 1900 nt, 2000 nt or longer. In other embodiments, the length of the first and/or the third segment comprises at least 10-19 nucleotides, 10-20 nucleotides; 19-35 nucleotides, 20-35 nucleotides; 30-45 nucleotides; 40-50 nucleotides; 50-100 nucleotides; 100-300 nucleotides; about 500-700 nucleotides; about 700-900 nucleotides; about 900-1100 nucleotides; about 1300-1500 nucleotides; about 1500-1700 nucleotides; about 1700-1900 nucleotides; about 1900-2100 nucleotides; about 2100-2300 nucleotides; or about 2300-2500 nucleotides. See, for example, International Publication No. WO 02/00904.
[0071] The disclosed hairpin molecules or double-stranded RNA molecules may have more than one disclosed sequence or active fragments or variants, or complements thereof, found in the same portion of the RNA molecule. For example, in a chimeric hairpin structure, the first segment of a hairpin molecule comprises two polynucleotide sections, each with a different disclosed sequence. For example, reading from one terminus of the hairpin, the first segment is composed of sequences from two separate genes (A followed by B). This first segment is followed by the second segment, the loop portion of the hairpin. The loop segment is followed by the third segment, where the complementary strands of the sequences in the first segment are found (B* followed by A*) in forming the stem-loop, hairpin structure, the stem contains SeqA-A* at the distal end of the stem and SeqB-B* proximal to the loop region.
[0072] In certain embodiments, the first and the third segment comprise at least 20 nucleotides having at least 85% complementary to the first segment. In still other embodiments, the first and the third segments which form the stem-loop structure of the hairpin comprise 3' or 5' overhang regions having unpaired nucleotide residues.
[0073] In certain embodiments, the sequences used in the first, the second, and/or the third segments comprise domains that are designed to have sufficient sequence identity to a target polynucleotide of interest and thereby have the ability to decrease the level of expression of the target polynucleotide. The specificity of the inhibitory RNA transcripts is therefore generally conferred by these domains of the silencing element. Thus, in some embodiments, the first, second and/or third segment of the silencing element comprise a domain having at least 10, at least 15, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 40, at least 50, at least 100, at least 200, at least 300, at least 500, at least 1000, or more than 1000 nucleotides that share sufficient sequence identity to the target polynucleotide to allow for a decrease in expression levels of the target polynucleotide when expressed in an appropriate cell. In other embodiments, the domain is between about 15 to 50 nucleotides, about 19-35 nucleotides, about 20-35 nucleotides, about 25-50 nucleotides, about 19 to 75 nucleotides, about 20 to 75 nucleotides, about 40-90 nucleotides about 15-100 nucleotides, 10-100 nucleotides, about 10 to about 75 nucleotides, about 10 to about 50 nucleotides, about 10 to about 40 nucleotides, about 10 to about 35 nucleotides, about 10 to about 30 nucleotides, about 10 to about 25 nucleotides, about 10 to about 20 nucleotides, about 10 to about 19 nucleotides, about 50 nucleotides to about 100 nucleotides, about 100 nucleotides to about 150 nucleotides, about 150 nucleotides to about 200 nucleotides, about 200 nucleotides to about 250 nucleotides, about 250 nucleotides to about 300 nucleotides, about 300 nucleotides to about 350 nucleotides, about 350 nucleotides to about 400 nucleotides, about 400 nucleotide to about 500 nucleotides or longer. In other embodiments, the length of the first and/or the third segment comprises at least 10-20 nucleotides, at least 10-19 nucleotides, 20-35 nucleotides, 30-45 nucleotides, 40-50 nucleotides, 50-100 nucleotides, or about 100-300 nucleotides.
[0074] In certain embodiments, a domain of the first, the second, and/or the third segment has 100% sequence identity to the target polynucleotide. In other embodiments, the domain of the first, the second and/or the third segment having homology to the target polynucleotide have at least 50%, 60%, 70%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or greater sequence identity to a region of the target polynucleotide. The sequence identity of the domains of the first, the second and/or the third segments complementary to a target polynucleotide need only be sufficient to decrease expression of the target polynucleotide of interest. See, for example, Chuang and Meyerowitz (2000) Proc. Natl. Acad. Sci. USA 97:4985-4990; Stoutjesdijk et al. (2002) Plant Physiol. 129:1723-1731; Waterhouse and Helliwell (2003) Nat. Rev. Genet. 4:29-38; Pandolfini et al. BMC Biotechnology 3:7, and U.S. Patent Publication No. 20030175965; each of which is herein incorporated by reference. A transient assay for the efficiency of hpRNA constructs to silence gene expression in vivo has been described by Panstruga et al. (2003) Mol. Biol. Rep. 30:135-140.
[0075] The amount of complementarity shared between the first, second, and/or third segment and the target polynucleotide or the amount of complementarity shared between the first segment and the third segment (i.e., the stem of the hairpin structure) may vary depending on the organism in which gene expression is to be controlled. Some organisms or cell types may require exact pairing or 100% identity, while other organisms or cell types may tolerate some mismatching. In some cells, for example, a single nucleotide mismatch in the targeting sequence abrogates the ability to suppress gene expression. In these cells, the disclosed suppression cassettes can be used to target the suppression of mutant genes, for example, oncogenes whose transcripts comprise point mutations and therefore they can be specifically targeted using the methods and compositions disclosed herein without altering the expression of the remaining wild-type allele. In other organisms, holistic sequence variability may be tolerated as long as some 22 nt region of the sequence is represented in 100% homology between target polynucleotide and the suppression cassette.
[0076] Any region of the target polynucleotide can be used to design a domain of the silencing element that shares sufficient sequence identity to allow expression of the hairpin transcript to decrease the level of the target polynucleotide. For instance, a domain may be designed to share sequence identity to the 5' untranslated region of the target polynucleotide(s), the 3' untranslated region of the target polynucleotide(s), exonic regions of the target polynucleotide(s), intronic regions of the target polynucleotide(s), and any combination thereof. In certain embodiments, a domain of the silencing element shares sufficient identity, homology, or is complementary to at least about 15, 16, 17, 18, 19, 20, 22, 25 or 30 consecutive nucleotides from about nucleotides 1-50, 25-75, 75-125, 50-100, 125-175, 175-225, 100-150, 150-200, 200-250, 225-275, 275-325, 250-300, 325-375, 375-425, 300-350, 350-400, 425-475, 400-450, 475-525, 450-500, 525-575, 575-625, 550-600, 625-675, 675-725, 600-650, 625-675, 675-725, 650-700, 725-825, 825-875, 750-800, 875-925, 925-975, 850-900, 925-975, 975-1025, 950-1000, 1000-1050, 1025-1075, 1075-1125, 1050-1100, 1125-1175, 1100-1200, 1175-1225, 1225-1275, 1200-1300, 1325-1375, 1375-1425, 1300-1400, 1425-1475, 1475-1525, 1400-1500, 1525-1575, 1575-1625, 1625-1675, 1675-1725, 1725-1775, 1775-1825, 1825-1875, 1875-1925, 1925-1975, 1975-2025, 2025-2075, 2075-2125, 2125-2175, 2175-2225, 1500-1600, 1600-1700, 1700-1800, 1800-1900, 1900-2000 of the target sequence. In some instances to optimize the siRNA sequences employed in the hairpin, the synthetic oligodeoxyribonucleotide/RNAse H method can be used to determine sites on the target mRNA that are in a conformation that is susceptible to RNA silencing. See, for example, Vickers et al. (2003) J. Biol. Chem 278:7108-7118 and Yang et al. (2002) Proc. Natl. Acad. Sci. USA 99:9442-9447, herein incorporated by reference. These studies indicate that there is a significant correlation between the RNase-H-sensitive sites and sites that promote efficient siRNA-directed mRNA degradation.
[0077] The hairpin silencing element may also be designed such that the sense sequence or the antisense sequence do not correspond to a target polynucleotide. In this embodiment, the sense and antisense sequence flank a loop sequence that comprises a nucleotide sequence corresponding to all or part of the target polynucleotide. Thus, it is the loop region that determines the specificity of the RNA interference. See, for example, WO 02/00904.
[0078] In addition, transcriptional gene silencing (TGS) may be accomplished through use of a hairpin suppression element where the inverted repeat of the hairpin shares sequence identity with the promoter region of a target polynucleotide to be silenced. See, for example, Aufsatz et al. (2002) PNAS 99 (Suppl. 4):16499-16506 and Mette et al. (2000) EMBO J 19(19):5194-5201.
[0079] In other embodiments, the silencing element can comprise a small RNA (sRNA). sRNAs can comprise both micro RNA (miRNA) and short-interfering RNA (siRNA) (Meister and Tuschl (2004) Nature 431:343-349 and Bonetta et al. (2004) Nature Methods 1:79-86). miRNAs are regulatory agents comprising about 19 to about 24 ribonucleotides in length which are highly efficient at inhibiting the expression of target polynucleotides. See, for example Javier et al. (2003) Nature 425: 257-263. For miRNA interference, the silencing element can be designed to express a dsRNA molecule that forms a hairpin structure or partially base-paired structure containing a 19, 20, 21, 22, 23, 24 or 25 nucleotide sequence that is complementary to the target polynucleotide of interest. The miRNA can be synthetically made, or transcribed as a longer RNA which is subsequently cleaved to produce the active miRNA. Specifically, the miRNA can comprise 19 nucleotides of the sequence having homology to a target polynucleotide in sense orientation and 19 nucleotides of a corresponding antisense sequence that is complementary to the sense sequence. The miRNA can be an "artificial miRNA" or "amiRNA" which comprises a miRNA sequence that is synthetically designed to silence a target sequence.
[0080] When expressing an miRNA the final (mature) miRNA is present in a duplex in a precursor backbone structure, the two strands being referred to as the miRNA (the strand that will eventually base pair with the target) and miRNA*(star sequence). It has been demonstrated that miRNAs can be transgenically expressed and target genes of interest for efficient silencing (Highly specific gene silencing by artificial microRNAs in Arabidopsis Schwab R, Ossowski S, Riester M, Warthmann N, Weigel D. Plant Cell. 2006 May; 18(5):1121-33. Epub 2006 Mar. 10; and Expression of artificial microRNAs in transgenic Arabidopsis thaliana confers virus resistance. Niu Q W, Lin S S, Reyes J L, Chen K C, Wu H W, Yeh S D, Chua N H. Nat Biotechnol. 2006 November; 24(11):1420-8. Epub 2006 Oct. 22. Erratum in: Nat Biotechnol. 2007 February; 25(2):254.).
[0081] The silencing element for miRNA interference comprises a miRNA primary sequence. The miRNA primary sequence comprises a DNA sequence having the miRNA and star sequences separated by a loop as well as additional sequences flanking this region that are important for processing. When expressed as an RNA, the structure of the primary miRNA is such as to allow for the formation of a hairpin RNA structure that can be processed into a mature miRNA. In some embodiments, the miRNA backbone comprises a genomic or cDNA miRNA precursor sequence, wherein said sequence comprises a native primary in which a heterologous (artificial) mature miRNA and star sequence are inserted.
[0082] As used herein, a "star sequence" is the sequence within a miRNA precursor backbone that is complementary to the miRNA and forms a duplex with the miRNA to form the stem structure of a hairpin RNA. In some embodiments, the star sequence can comprise less than 100% complementarity to the miRNA sequence. Alternatively, the star sequence can comprise at least 99%, 98%, 97%, 96%, 95%, 90%, 85%, 80% or lower sequence complementarity to the miRNA sequence as long as the star sequence has sufficient complementarity to the miRNA sequence to form a double stranded structure. In still further embodiments, the star sequence comprises a sequence having 1, 2, 3, 4, 5 or more mismatches with the miRNA sequence and still has sufficient complementarity to form a double stranded structure with the miRNA sequence resulting in production of miRNA and suppression of the target sequence.
[0083] The miRNA precursor backbones can be from any plant. In some embodiments, the miRNA precursor backbone is from a monocot. In other embodiments, the miRNA precursor backbone is from a dicot. In further embodiments, the backbone is from maize or soybean. MicroRNA precursor backbones have been described previously. For example, US20090155910A1 (WO 2009/079532) discloses the following soybean miRNA precursor backbones: 156c, 159, 166b, 168c, 396b and 398b, and US20090155909A1 (WO 2009/079548) discloses the following maize miRNA precursor backbones: 159c, 164h, 168a, 169r, and 396h.
[0084] Thus, the primary miRNA can be altered to allow for efficient insertion of heterologous miRNA and star sequences within the miRNA precursor backbone. In such instances, the miRNA segment and the star segment of the miRNA precursor backbone are replaced with the heterologous miRNA and the heterologous star sequences, designed to target any sequence of interest, using a PCR technique and cloned into an expression construct. It is recognized that there could be alterations to the position at which the artificial miRNA and star sequences are inserted into the backbone. Detailed methods for inserting the miRNA and star sequence into the miRNA precursor backbone are described in, for example, US Patent Applications 20090155909A1 and US20090155910A1.
[0085] When designing a miRNA sequence and star sequence, various design choices can be made. See, for example, Schwab R, et al. (2005) Dev Cell 8: 517-27. In non-limiting embodiments, the miRNA sequences disclosed herein can have a "U" at the 5'-end, a "C" or "G" at the 19th nucleotide position, and an "A" or "U" at the 10th nucleotide position. In other embodiments, the miRNA design is such that the miRNA have a high free delta-G as calculated using the ZipFold algorithm (Markham, N. R. & Zuker, M. (2005) Nucleic Acids Res. 33: W577-W581.) Optionally, a one base pair change can be added within the 5' portion of the miRNA so that the sequence differs from the target sequence by one nucleotide.
[0086] The methods and compositions disclosed herein employ DNA constructs that when transcribed "form" a silencing element, such as a dsRNA molecule. The methods and compositions also may comprise a host cell comprising the DNA construct encoding a silencing element. In another embodiment, The methods and compositions also may comprise a transgenic plant comprising the DNA construct encoding a silencing element. Accordingly, the heterologous polynucleotide being expressed need not form the dsRNA by itself, but can interact with other sequences in the plant cell or in the pest gut after ingestion to allow the formation of the dsRNA. For example, a chimeric polynucleotide that can selectively silence the target polynucleotide can be generated by expressing a chimeric construct comprising the target sequence for a miRNA or siRNA to a sequence corresponding to all or part of the gene or genes to be silenced. In this embodiment, the dsRNA is "formed" when the target for the miRNA or siRNA interacts with the miRNA present in the cell. The resulting dsRNA can then reduce the level of expression of the gene or genes to be silenced. See, for example, US Application Publication 2007-0130653, entitled "Methods and Compositions for Gene Silencing". The construct can be designed to have a target for an endogenous miRNA or alternatively, a target for a heterologous and/or synthetic miRNA can be employed in the construct. If a heterologous and/or synthetic miRNA is employed, it can be introduced into the cell on the same nucleotide construct as the chimeric polynucleotide or on a separate construct. As discussed elsewhere herein, any method can be used to introduce the construct comprising the heterologous miRNA.
IV. Variants and Fragments
[0087] By "fragment" is intended a portion of the polynucleotide or a portion of the amino acid sequence and hence protein encoded thereby. Fragments of a polynucleotide may encode protein fragments that retain the biological activity of the native protein. Alternatively, fragments of a polynucleotide that are useful as a silencing element do not need to encode fragment proteins that retain biological activity. Thus, fragments of a nucleotide sequence may range from at least about 10, about 15, about 16, about 17, about 18, about 19, nucleotides, about 20 nucleotides, about 22 nucleotides, about 50 nucleotides, about 75 nucleotides, about 100 nucleotides, 200 nucleotides, 300 nucleotides, 400 nucleotides, 500 nucleotides, 600 nucleotides, 700 nucleotides and up to and including one nucleotide less than the full-length polynucleotide employed. Alternatively, fragments of a nucleotide sequence may range from 1-50, 25-75, 75-125, 50-100, 125-175, 175-225, 100-150, 100-300, 150-200, 200-250, 225-275, 275-325, 250-300, 325-375, 375-425, 300-350, 350-400, 425-475, 400-450, 475-525, 450-500, 525-575, 575-625, 550-600, 625-675, 675-725, 600-650, 625-675, 675-725, 650-700, 725-825, 825-875, 750-800, 875-925, 925-975, 850-900, 925-975, 975-1025, 950-1000, 1000-1050, 1025-1075, 1075-1125, 1050-1100, 1125-1175, 1100-1200, 1175-1225, 1225-1275, 1200-1300, 1325-1375, 1375-1425, 1300-1400, 1425-1475, 1475-1525, 1400-1500, 1525-1575, 1575-1625, 1625-1675, 1675-1725, 1725-1775, 1775-1825, 1825-1875, 1875-1925, 1925-1975, 1975-2025, 2025-2075, 2075-2125, 2125-2175, 2175-2225, 1500-1600, 1600-1700, 1700-1800, 1800-1900, 1900-2000 of any one of SEQ ID NOS.: 1-53 or 107-254, or variants and fragments thereof, and complements thereof. Methods to assay for the activity of a desired silencing element are described elsewhere herein.
[0088] "Variants" is intended to mean substantially similar sequences. For polynucleotides, a variant comprises a deletion and/or addition of one or more nucleotides at one or more internal sites within the native polynucleotide and/or a substitution of one or more nucleotides at one or more sites in the native polynucleotide. A variant of a polynucleotide that is useful as a silencing element will retain the ability to reduce expression of the target polynucleotide and, in some embodiments, thereby control a plant insect pest of interest. As used herein, a "native" polynucleotide or polypeptide comprises a naturally occurring nucleotide sequence or amino acid sequence, respectively. For polynucleotides, conservative variants include those sequences that, because of the degeneracy of the genetic code, encode the amino acid sequence of one of the disclosed polypeptides. Variant polynucleotides also include synthetically derived polynucleotide, such as those generated, for example, by using site-directed mutagenesis, but continue to retain the desired activity. Generally, variants of a particular disclosed polynucleotide (i.e., a silencing element) will have at least about 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity to that particular polynucleotide as determined by sequence alignment programs and parameters described elsewhere herein.
[0089] Variants of a particular disclosed polynucleotide (i.e., the reference polynucleotide) can also be evaluated by comparison of the percent sequence identity between the polypeptide encoded by a variant polynucleotide and the polypeptide encoded by the reference polynucleotide. Percent sequence identity between any two polypeptides can be calculated using sequence alignment programs and parameters described elsewhere herein. Where any given pair of disclosed polynucleotides employed is evaluated by comparison of the percent sequence identity shared by the two polypeptides they encode, the percent sequence identity between the two encoded polypeptides is at least about 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence identity.
[0090] The following terms are used to describe the sequence relationships between two or more polynucleotides or polypeptides: (a) "reference sequence", (b) "comparison window", (c) "sequence identity", and, (d) "percentage of sequence identity."
[0091] (a) As used herein, "reference sequence" is a defined sequence used as a basis for sequence comparison. A reference sequence may be a subset or the entirety of a specified sequence; for example, as a segment of a full-length cDNA or gene sequence, or the complete cDNA or gene sequence.
[0092] (b) As used herein, "comparison window" makes reference to a contiguous and specified segment of a polynucleotide sequence, wherein the polynucleotide sequence in the comparison window may comprise additions or deletions (i.e., gaps) compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two polynucleotides. Generally, the comparison window is at least 20 contiguous nucleotides in length, and optionally can be 30, 40, 50, 100, or longer. Those of skill in the art understand that to avoid a high similarity to a reference sequence due to inclusion of gaps in the polynucleotide sequence a gap penalty is typically introduced and is subtracted from the number of matches.
[0093] Unless otherwise stated, sequence identity/similarity values provided herein refer to the value obtained using GAP Version 10 using the following parameters: % identity and % similarity for a nucleotide sequence using GAP Weight of 50 and Length Weight of 3, and the nwsgapdna.cmp scoring matrix; % identity and % similarity for an amino acid sequence using GAP Weight of 8 and Length Weight of 2, and the BLOSUM62 scoring matrix; or any equivalent program thereof. By "equivalent program" is intended any sequence comparison program that, for any two sequences in question, generates an alignment having identical nucleotide or amino acid residue matches and an identical percent sequence identity when compared to the corresponding alignment generated by GAP Version 10.
[0094] (c) As used herein, "sequence identity" or "identity" in the context of two polynucleotides or polypeptide sequences makes reference to the residues in the two sequences that are the same when aligned for maximum correspondence over a specified comparison window. When percentage of sequence identity is used in reference to proteins it is recognized that residue positions which are not identical often differ by conservative amino acid substitutions, where amino acid residues are substituted for other amino acid residues with similar chemical properties (e.g., charge or hydrophobicity) and therefore do not change the functional properties of the molecule. When sequences differ in conservative substitutions, the percent sequence identity may be adjusted upwards to correct for the conservative nature of the substitution. Sequences that differ by such conservative substitutions are said to have "sequence similarity" or "similarity". Means for making this adjustment are well known to those of skill in the art. Typically this involves scoring a conservative substitution as a partial rather than a full mismatch, thereby increasing the percentage sequence identity. Thus, for example, where an identical amino acid is given a score of 1 and a non-conservative substitution is given a score of zero, a conservative substitution is given a score between zero and 1. The scoring of conservative substitutions is calculated, e.g., as implemented in the program PC/GENE (Intelligenetics, Mountain View, Calif.).
[0095] (d) As used herein, "percentage of sequence identity" means the value determined by comparing two optimally aligned sequences over a comparison window, wherein the portion of the polynucleotide sequence in the comparison window may comprise additions or deletions (i.e., gaps) as compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two sequences. The percentage is calculated by determining the number of positions at which the identical nucleic acid base or amino acid residue occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the window of comparison, and multiplying the result by 100 to yield the percentage of sequence identity.
[0096] A method is further provided for identifying a silencing element from the target polynucleotides set forth in SEQ ID NOS.: 1-53 or 107-254, or variants and fragments thereof, and complements thereof. Such methods comprise obtaining a candidate fragment of any one of SEQ ID NOS.: 1-53 or 107-254, or variants and fragments thereof, and complements thereof, which is of sufficient length to act as a silencing element and thereby reduce the expression of the target polynucleotide and/or control a desired pest; expressing said candidate polynucleotide fragment in an appropriate expression cassette to produce a candidate silencing element and determining is said candidate polynucleotide fragment has the activity of a silencing element and thereby reduce the expression of the target polynucleotide and/or controls a desired pest. Methods of identifying such candidate fragments based on the desired pathway for suppression, in light of the teachings provided herein, are known. For example, various bioinformatics programs can be employed to identify the region of the target polynucleotides that could be exploited to generate a silencing element. See, for example, Elbahir et al. (2001) Genes and Development 15:188-200, Schwartz et al. (2003) Cell 115:199-208, Khvorova et al. (2003) Cell 115:209-216. See also, siRNA at Whitehead (jura.wi.mit.edu/bioc/siRNAext/) which calculates the binding energies for both sense and antisense siRNAs. See, also genscript.com/ssl-bin/app/rnai?op=known; Block-iT.TM. RNAi designer from Invitrogen and GenScript siRNA Construct Builder. In various aspects, it is to be understand that the term " . . . SEQ ID NOS.: 1-53 or 107-254, or variants or fragments thereof, or complements thereof . . . " is intended to mean that the disclosed sequences comprise SEQ ID NOS.: 1-53 or 107-254, and/or fragments of SEQ ID NOS.: 1-53 or 107-254, and/or variants of SEQ ID NOS.: 1-53 or 107-254, and/or the complements of SEQ ID NOS.: 1-53 or 107-254, the variants of SEQ ID NOS.: 1-53 or 107-254, and/or the fragments of SEQ ID NOS.: 1-53 or 107-254, individually (or) or inclusive of some or all listed sequences.
V. DNA Constructs
[0097] The use of the term "polynucleotide" is not intended to be limiting to polynucleotides comprising DNA. Those of ordinary skill in the art will recognize that polynucleotides can comprise ribonucleotides and combinations of ribonucleotides and deoxyribonucleotides. Such deoxyribonucleotides and ribonucleotides include both naturally occurring molecules and synthetic analogues. The disclosed polynucleotides also encompass all forms of sequences including, but not limited to, single-stranded forms, double-stranded forms, hairpins, stem-and-loop structures, and the like.
[0098] The polynucleotide encoding the silencing element or in certain embodiments employed in the disclosed methods and compositions can be provided in expression cassettes for expression in a plant or organism of interest. It is recognized that multiple silencing elements including multiple identical silencing elements, multiple silencing elements targeting different regions of the target sequence, or multiple silencing elements from different target sequences can be used. In this embodiment, it is recognized that each silencing element may be encoded by a single or separate cassette, DNA construct, or vector. As discussed, any means of providing the silencing element is contemplated. A plant or plant cell can be transformed with a single cassette comprising DNA encoding one or more silencing elements or separate cassettes encoding a silencing element can be used to transform a plant or plant cell or host cell. Likewise, a plant transformed with one component can be subsequently transformed with the second component. One or more DNA constructs encoding silencing elements can also be brought together by sexual crossing. That is, a first plant comprising one component is crossed with a second plant comprising the second component. Progeny plants from the cross will comprise both components.
[0099] The expression cassette can include 5' and 3' regulatory sequences operably linked to the polynucleotide of the invention. "Operably linked" is intended to mean a functional linkage between two or more elements. For example, an operable linkage between a polynucleotide of the invention and a regulatory sequence (i.e., a promoter) is a functional link that allows for expression of the polynucleotide disclosed herein. Operably linked elements may be contiguous or non-contiguous. When used to refer to the joining of two protein coding regions, by operably linked is intended that the coding regions are in the same reading frame. The cassette may additionally contain at least one additional polynucleotide to be cotransformed into the organism. Alternatively, the additional polypeptide(s) can be provided on multiple expression cassettes. Expression cassettes can be provided with a plurality of restriction sites and/or recombination sites for insertion of the polynucleotide to be under the transcriptional regulation of the regulatory regions. The expression cassette may additionally contain selectable marker genes.
[0100] The expression cassette can include in the 5'-3' direction of transcription, a transcriptional and translational initiation region (i.e., a promoter), a polynucleotide encoding the silencing element employed in the methods and compositions of the invention, and a transcriptional and translational termination region (i.e., termination region) functional in plants. In other embodiment, the double stranded RNA is expressed from a suppression cassette. Such a cassette can comprise two convergent promoters that drive transcription of an operably linked silencing element. "Convergent promoters" refers to promoters that are oriented on either terminus of the operably linked polynucleotide encoding the silencing element such that each promoter drives transcription of the silencing element in opposite directions, yielding two transcripts. In such embodiments, the convergent promoters allow for the transcription of the sense and anti-sense strand and thus allow for the formation of a dsRNA. Such a cassette may also comprise two divergent promoters that drive transcription of one or more operably linked polynucleotides encoding the silencing elements. "Divergent promoters" refers to promoters that are oriented in opposite directions of each other, driving transcription of the one or more polynucleotides encoding the silencing elements in opposite directions. In such embodiments, the divergent promoters allow for the transcription of the sense and antisense strands and allow for the formation of a dsRNA. In such embodiments, the divergent promoters also allow for the transcription of at least two separate hairpin RNAs. In another embodiment, one cassette comprising two or more polynucleotides encoding the silencing elements under the control of two separate promoters in the same orientation is present in a construct. In another embodiment, two or more individual cassettes, each comprising at least one polynucleotide encoding the silencing element under the control of a promoter, are present in a construct in the same orientation.
[0101] The regulatory regions (i.e., promoters, transcriptional regulatory regions, and translational termination regions) and/or the polynucleotides disclosed herein may be native/analogous to the host cell or to each other. Alternatively, the regulatory regions and/or the polynucleotide disclosed herein may be heterologous to the host cell or to each other. As used herein, "heterologous" in reference to a sequence is a sequence that originates from a foreign species, or, if from the same species, is substantially modified from its native form in composition and/or genomic locus by deliberate human intervention. For example, a promoter operably linked to a heterologous polynucleotide is from a species different from the species from which the polynucleotide was derived, or, if from the same/analogous species, one or both are substantially modified from their original form and/or genomic locus, or the promoter is not the native promoter for the operably linked polynucleotide. As used herein, a chimeric gene comprises a coding sequence operably linked to a transcription initiation region that is heterologous to the coding sequence.
[0102] The termination region may be native with the transcriptional initiation region, may be native with the operably linked polynucleotide encoding the silencing element, may be native with the plant host, or may be derived from another source (i.e., foreign or heterologous) to the promoter, the polynucleotide encoding the silencing element, the plant host, or any combination thereof. Convenient termination regions are available from the Ti-plasmid of A. tumefaciens, such as the octopine synthase and nopaline synthase termination regions. See also Guerineau et al. (1991) Mol. Gen. Genet. 262:141-144; Proudfoot (1991) Cell 64:671-674; Sanfacon et al. (1991) Genes Dev. 5:141-149; Mogen et al. (1990) Plant Cell 2:1261-1272; Munroe et al. (1990) Gene 91:151-158; Ballas et al. (1989) Nucleic Acids Res. 17:7891-7903; and Joshi et al. (1987) Nucleic Acids Res. 15:9627-9639.
[0103] Additional sequence modifications are known to enhance gene expression in a cellular host. These include elimination of sequences encoding spurious polyadenylation signals, exon-intron splice site signals, transposon-like repeats, and other such well-characterized sequences that may be deleterious to gene expression. The G-C content of the sequence may be adjusted to levels average for a given cellular host, as calculated by reference to known genes expressed in the host cell. When possible, the sequence is modified to avoid predicted hairpin secondary mRNA structures.
[0104] In preparing the expression cassette, the various DNA fragments may be manipulated, so as to provide for the DNA sequences in the proper orientation and, as appropriate, in the proper reading frame. Toward this end, adapters or linkers may be employed to join the DNA fragments or other manipulations may be involved to provide for convenient restriction sites, removal of superfluous DNA, removal of restriction sites, or the like. For this purpose, in vitro mutagenesis, primer repair, restriction, annealing, resubstitutions, e.g., transitions and transversions, may be involved.
[0105] A number of promoters can be used in the practice of the invention. The promoters can be selected based on the desired outcome. The nucleic acids can be combined with constitutive, tissue-preferred, inducible, or other promoters for expression in the host organism.
[0106] Such constitutive promoters include, for example, the core promoter of the Rsyn7 promoter and other constitutive promoters disclosed in WO 99/43838 and U.S. Pat. No. 6,072,050; the core CaMV 35S promoter (Odell et al. (1985) Nature 313:810-812); rice actin (McElroy et al. (1990) Plant Cell 2:163-171); ubiquitin (Christensen et al. (1989) Plant Mol. Biol. 12:619-632 and Christensen et al. (1992) Plant Mol. Biol. 18:675-689); pEMU (Last et al. (1991) Theor. Appl. Genet. 81:581-588); MAS (Velten et al. (1984) EMBO J. 3:2723-2730); ALS promoter (U.S. Pat. No. 5,659,026), and the like. Other constitutive promoters include, for example, U.S. Pat. Nos. 5,608,149; 5,608,144; 5,604,121; 5,569,597; 5,466,785; 5,399,680; 5,268,463; 5,608,142; and 6,177,611.
[0107] Depending on the desired outcome, it may be beneficial to express the gene from an inducible promoter. An inducible promoter, for instance, a pathogen-inducible promoter could also be employed. Such promoters include those from pathogenesis-related proteins (PR proteins), which are induced following infection by a pathogen; e.g., PR proteins, SAR proteins, beta-1,3-glucanase, chitinase, etc. See, for example, Redolfi et al. (1983) Neth. J. Plant Pathol. 89:245-254; Uknes et al. (1992) Plant Cell 4:645-656; and Van Loon (1985) Plant Mol. Virol. 4:111-116. See also WO 99/43819.
[0108] Additionally, as pathogens find entry into plants through wounds or insect damage, a wound-inducible promoter may be used in the constructions of the invention. Such wound-inducible promoters include potato proteinase inhibitor (pin II) gene (Ryan (1990) Ann. Rev. Phytopath. 28:425-449; Duan et al. (1996) Nature Biotechnology 14:494-498); wun1 and wun2, U.S. Pat. No. 5,428,148; winl and wing (Stanford et al. (1989) Mol. Gen. Genet. 215:200-208); systemin (McGurl et al. (1992) Science 225:1570-1573); WIP1 (Rohmeier et al. (1993) Plant Mol. Biol. 22:783-792; Eckelkamp et al. (1993) FEBS Letters 323:73-76); MPI gene (Corderok et al. (1994) Plant J. 6(2):141-150); and the like.
[0109] Additionally, pathogen-inducible promoters may be employed in the methods and nucleotide constructs of the embodiments. Such pathogen-inducible promoters include those from pathogenesis-related proteins (PR proteins), which are induced following infection by a pathogen; e.g., PR proteins, SAR proteins, beta-1,3-glucanase, chitinase, etc. See, for example, Redolfi et al. (1983) Neth. J. Plant Pathol. 89: 245-254; Uknes et al. (1992) Plant Cell 4: 645-656; and Van Loon (1985) Plant Mol. Virol. 4: 111-116. See also WO 99/43819.
[0110] Of interest are promoters that are expressed locally at or near the site of pathogen infection. See, for example, Marineau et al. (1987) Plant Mol. Biol. 9:335-342; Matton et al. (1989) Molecular Plant-Microbe Interactions 2:325-331; Somsisch et al. (1986) Proc. Natl. Acad. Sci. USA 83:2427-2430; Somsisch et al. (1988) Mol. Gen. Genet. 2:93-98; and Yang (1996) Proc. Natl. Acad. Sci. USA 93:14972-14977. See also, Chen et al. (1996) Plant J. 10:955-966; Zhang et al. (1994) Proc. Natl. Acad. Sci. USA 91:2507-2511; Warner et al. (1993) Plant J. 3:191-201; Siebertz et al. (1989) Plant Cell 1:961-968; U.S. Pat. No. 5,750,386 (nematode-inducible). Of particular interest is the inducible promoter for the maize PRms gene, whose expression is induced by the pathogen Fusarium moniliforme (see, for example, Cordero et al. (1992) Physiol. Mol. Plant Path. 41:189-200).
[0111] Chemical-regulated promoters can be used to modulate the expression of a gene in a plant through the application of an exogenous chemical regulator. Depending upon the objective, the promoter may be a chemical-inducible promoter, where application of the chemical induces gene expression, or a chemical-repressible promoter, where application of the chemical represses gene expression. Chemical-inducible promoters are known in the art and include, but are not limited to, the maize In2-2 promoter, which is activated by benzenesulfonamide herbicide safeners, the maize GST promoter, which is activated by hydrophobic electrophilic compounds that are used as pre-emergent herbicides, and the tobacco PR-la promoter, which is activated by salicylic acid. Other chemical-regulated promoters of interest include steroid-responsive promoters (see, for example, the glucocorticoid-inducible promoter in Schena et al. (1991) Proc. Natl. Acad. Sci. USA 88:10421-10425 and McNellis et al. (1998) Plant J. 14(2):247-257) and tetracycline-inducible and tetracycline-repressible promoters (see, for example, Gatz et al. (1991) Mol. Gen. Genet. 227:229-237, and U.S. Pat. Nos. 5,814,618 and 5,789,156).
[0112] Tissue-preferred promoters can be utilized to target enhanced expression within a particular plant tissue. Tissue-preferred promoters include Yamamoto et al. (1997) Plant J. 12(2):255-265; Kawamata et al. (1997) Plant Cell Physiol. 38(7):792-803; Hansen et al. (1997) Mol. Gen Genet. 254(3):337-343; Russell et al. (1997) Transgenic Res. 6(2):157-168; Rinehart et al. (1996) Plant Physiol. 112(3):1331-1341; Van Camp et al. (1996) Plant Physiol. 112(2):525-535; Canevascini et al. (1996) Plant Physiol. 112(2):513-524; Yamamoto et al. (1994) Plant Cell Physiol. 35(5):773-778; Lam (1994) Results Probl. Cell Differ. 20:181-196; Orozco et al. (1993) Plant Mol Biol. 23(6):1129-1138; Matsuoka et al. (1993) Proc Natl. Acad. Sci. USA 90(20):9586-9590; and Guevara-Garcia et al. (1993) Plant J. 4(3):495-505. Such promoters can be modified, if necessary, for weak expression.
[0113] Leaf-preferred promoters are known in the art. See, for example, Yamamoto et al. (1997) Plant J. 12(2):255-265; Kwon et al. (1994) Plant Physiol. 105:357-67; Yamamoto et al. (1994) Plant Cell Physiol. 35(5):773-778; Gotor et al. (1993) Plant J. 3:509-18; Orozco et al. (1993) Plant Mol. Biol. 23(6):1129-1138; and Matsuoka et al. (1993) Proc. Natl. Acad. Sci. USA 90(20):9586-9590.
[0114] Root-preferred promoters are known and can be selected from the many available from the literature or isolated de novo from various compatible species. See, for example, Hire et al. (1992) Plant Mol. Biol. 20(2):207-218 (soybean root-specific glutamine synthetase gene); Keller and Baumgartner (1991) Plant Cell 3(10):1051-1061 (root-specific control element in the GRP 1.8 gene of French bean); Sanger et al. (1990) Plant Mol. Biol. 14(3):433-443 (root-specific promoter of the mannopine synthase (MAS) gene of Agrobacterium tumefaciens); and Miao et al. (1991) Plant Cell 3(1):11-22 (full-length cDNA clone encoding cytosolic glutamine synthetase (GS), which is expressed in roots and root nodules of soybean). See also Bogusz et al. (1990) Plant Cell 2(7):633-641, where two root-specific promoters isolated from hemoglobin genes from the nitrogen-fixing nonlegume Parasponia andersonii and the related non-nitrogen-fixing nonlegume Trema tomentosa are described. The promoters of these genes were linked to a .beta.-glucuronidase reporter gene and introduced into both the nonlegume Nicotiana tabacum and the legume Lotus corniculatus, and in both instances root-specific promoter activity was preserved. Leach and Aoyagi (1991) describe their analysis of the promoters of the highly expressed rolC and rolD root-inducing genes of Agrobacterium rhizogenes (see Plant Science (Limerick) 79(1):69-76). They concluded that enhancer and tissue-preferred DNA determinants are dissociated in those promoters. Teeri et al. (1989) used gene fusion to lacZ to show that the Agrobacterium T-DNA gene encoding octopine synthase is especially active in the epidermis of the root tip and that the TR2' gene is root specific in the intact plant and stimulated by wounding in leaf tissue, an especially desirable combination of characteristics for use with an insecticidal or larvicidal gene (see EMBO J. 8(2):343-350). The TR1' gene, fused to nptII (neomycin phosphotransferase II) showed similar characteristics. Additional root-preferred promoters include the VfENOD-GRP3 gene promoter (Kuster et al. (1995) Plant Mol. Biol. 29(4):759-772); and rolB promoter (Capana et al. (1994) Plant Mol. Biol. 25(4):681-691. See also U.S. Pat. Nos. 5,837,876; 5,750,386; 5,633,363; 5,459,252; 5,401,836; 5,110,732; and 5,023,179.
[0115] Seed-preferred" promoters include both "seed-specific" promoters (those promoters active during seed development such as promoters of seed storage proteins) as well as "seed-germinating" promoters (those promoters active during seed germination). See Thompson et al. (1989) BioEssays 10:108. Such seed-preferred promoters include, but are not limited to, Cim1 (cytokinin-induced message); cZ19B1 (maize 19 kDa zein); and milps (myo-inositol-1-phosphate synthase) (see U.S. Pat. No. 6,225,529, herein incorporated by reference). Gamma-zein and Glob-1 are endosperm-specific promoters. For dicots, seed-specific promoters include, but are not limited to, bean .quadrature.-phaseolin, napin, .quadrature.-conglycinin, soybean lectin, cruciferin, and the like. For monocots, seed-specific promoters include, but are not limited to, maize 15 kDa zein, 22 kDa zein, 27 kDa zein, g-zein, waxy, shrunken 1, shrunken 2, globulin 1, etc. See also WO 00/12733, where seed-preferred promoters from end1 and end2 genes are disclosed. A promoter that has "preferred" expression in a particular tissue is expressed in that tissue to a greater degree than in at least one other plant tissue. Some tissue-preferred promoters show expression almost exclusively in the particular tissue.
[0116] In an embodiment, the plant-expressed promoter is a vascular-specific promoter such as a phloem-specific promoter. A "vascular-specific" promoter, as used herein, is a promoter which is at least expressed in vascular cells, or a promoter which is preferentially expressed in vascular cells. Expression of a vascular-specific promoter need not be exclusively in vascular cells, expression in other cell types or tissues is possible. A "phloem-specific promoter" as used herein, is a plant-expressible promoter which is at least expressed in phloem cells, or a promoter which is preferentially expressed in phloem cells.
[0117] Expression of a phloem-specific promoter need not be exclusively in phloem cells, expression in other cell types or tissues, e.g., xylem tissue, is possible. In one embodiment of this invention, a phloem-specific promoter is a plant-expressible promoter at least expressed in phloem cells, wherein the expression in non-phloem cells is more limited (or absent) compared to the expression in phloem cells. Examples of suitable vascular-specific or phloem-specific promoters in accordance with this invention include but are not limited to the promoters selected from the group consisting of: the SCSV3, SCSV4, SCSV5, and SCSV7 promoters (Schunmann et al. (2003) Plant Functional Biology 30:453-60; the rolC gene promoter of Agrobacterium rhizogenes (Kiyokawa et al. (1994) Plant Physiology 104:801-02; Pandolfini et al. (2003) BioMedCentral (BMC) Biotechnology 3:7, (www.biomedcentral.com/1472-6750/3/7); Graham et al. (1997) Plant Mol. Biol. 33:729-35; Guivarc'h et al. (1996); Almon et al. (1997) Plant Physiol. 115:1599-607; the rolA gene promoter of Agrobacterium rhizogenes (Dehio et al. (1993) Plant Mol. Biol. 23:1199-210); the promoter of the Agrobacterium tumefaciens T-DNA gene 5 (Korber et al. (1991) EMBO J. 10:3983-91); the rice sucrose synthase RSs1 gene promoter (Shi et al. (1994) J. Exp. Bot. 45:623-31); the CoYMV or Commelina yellow mottle badnavirus promoter (Medberry et al. (1992) Plant Cell 4:185-92; Zhou et al. (1998) Chin. J. Biotechnol. 14:9-16); the CFDV or coconut foliar decay virus promoter (Rohde et al. (1994) Plant Mol. Biol. 27:623-28; Hehn and Rhode (1998) J. Gen. Virol. 79:1495-99); the RTBV or rice tungro bacilliform virus promoter (Yin and Beachy (1995) Plant J. 7:969-80; Yin et al. (1997) Plant J. 12:1179-80); the pea glutamin synthase GS3A gene (Edwards et al. (1990) Proc. Natl. Acad. Sci. USA 87:3459-63; Brears et al. (1991) Plant J. 1:235-44); the inv CD111 and inv CD141 promoters of the potato invertase genes (Hedley et al. (2000) J. Exp. Botany 51:817-21); the promoter isolated from Arabidopsis shown to have phloem-specific expression in tobacco by Kertbundit et al. (1991) Proc. Natl. Acad. Sci. USA 88:5212-16); the VAHOX1 promoter region (Tornero et al. (1996) Plant J. 9:639-48); the pea cell wall invertase gene promoter (Zhang et al. (1996) Plant Physiol. 112:1111-17); the promoter of the endogenous cotton protein related to chitinase of US published patent application 20030106097, an acid invertase gene promoter from carrot (Ramloch-Lorenz et al. (1993) The Plant J. 4:545-54); the promoter of the sulfate transporter gene, Sultr1; 3 (Yoshimoto et al. (2003) Plant Physiol. 131:1511-17); a promoter of a sucrose synthase gene (Nolte and Koch (1993) Plant Physiol. 101:899-905); and the promoter of a tobacco sucrose transporter gene (Kuhn et al. (1997) Science 275-1298-1300).
[0118] Possible promoters also include the Black Cherry promoter for Prunasin Hydrolase (PH DL1.4 PRO) (U.S. Pat. No. 6,797,859), Thioredoxin H promoter from cucumber and rice (Fukuda A et al. (2005). Plant Cell Physiol. 46(11):1779-86), Rice (RSs1) (Shi, T. Wang et al. (1994). J. Exp. Bot. 45(274): 623-631) and maize sucrose synthase-1 promoters (Yang., N-S. et al. (1990) PNAS 87:4144-4148), PP2 promoter from pumpkin Guo, H. et al. (2004) Transgenic Research 13:559-566), At SUC2 promoter (Truernit, E. et al. (1995) Planta 196(3):564-70., At SAM-1 (S-adenosylmethionine synthetase) (Mijnsbrugge K V. et al. (1996) Plant Cell. Physiol. 37(8): 1108-1115), and the Rice tungro bacilliform virus (RTBV) promoter (Bhattacharyya-Pakrasi et al. (1993) Plant J. 4(1):71-79).
[0119] Where low level expression is desired, weak promoters will be used. Generally, the term "weak promoter" as used herein refers to a promoter that drives expression of a coding sequence at a low level. By low level expression at levels of about 1/1000 transcripts to about 1/100,000 transcripts to about 1/500,000 transcripts is intended. Alternatively, it is recognized that the term "weak promoters" also encompasses promoters that drive expression in only a few cells and not in others to give a total low level of expression. Where a promoter drives expression at unacceptably high levels, portions of the promoter sequence can be deleted or modified to decrease expression levels.
[0120] Such weak constitutive promoters include, for example the core promoter of the Rsyn7 promoter (WO 99/43838 and U.S. Pat. No. 6,072,050), the core 35S CaMV promoter, and the like. Other constitutive promoters include, for example, those disclosed in U.S. Pat. Nos. 5,608,149; 5,608,144; 5,604,121; 5,569,597; 5,466,785; 5,399,680; 5,268,463; 5,608,142; and 6,177,611.
[0121] The expression cassette can also comprise a selectable marker gene for the selection of transformed cells. Selectable marker genes are utilized for the selection of transformed cells or tissues. Marker genes include genes encoding antibiotic resistance, such as those encoding neomycin phosphotransferase II (NEO) and hygromycin phosphotransferase (HPT), as well as genes conferring resistance to herbicidal compounds, such as glufosinate ammonium, bromoxynil, imidazolinones, and 2,4-dichlorophenoxyacetate (2,4-D). Additional selectable markers include phenotypic markers such as .beta.-galactosidase and fluorescent proteins such as green fluorescent protein (GFP) (Su et al. (2004) Biotechnol Bioeng 85:610-9 and Fetter et al. (2004) Plant Cell 16:215-28), cyan florescent protein (CYP) (Bolte et al. (2004) J. Cell Science 117:943-54 and Kato et al. (2002) Plant Physiol 129:913-42), and yellow florescent protein (PhiYFP.TM. from Evrogen, see, Bolte et al. (2004) J. Cell Science 117:943-54). For additional selectable markers, see generally, Yarranton (1992) Curr. Opin. Biotech. 3:506-511; Christopherson et al. (1992) Proc. Natl. Acad. Sci. USA 89:6314-6318; Yao et al. (1992) Cell 71:63-72; Reznikoff (1992) Mol. Microbiol. 6:2419-2422; Barkley et al. (1980) in The Operon, pp. 177-220; Hu et al. (1987) Cell 48:555-566; Brown et al. (1987) Cell 49:603-612; Figge et al. (1988) Cell 52:713-722; Deuschle et al. (1989) Proc. Natl. Acad. Sci. USA 86:5400-5404; Fuerst et al. (1989) Proc. Natl. Acad. Sci. USA 86:2549-2553; Deuschle et al. (1990) Science 248:480-483; Gossen (1993) Ph.D. Thesis, University of Heidelberg; Reines et al. (1993) Proc. Natl. Acad. Sci. USA 90:1917-1921; Labow et al. (1990) Mol. Cell. Biol. 10:3343-3356; Zambretti et al. (1992) Proc. Natl. Acad. Sci. USA 89:3952-3956; Bairn et al. (1991) Proc. Natl. Acad. Sci. USA 88:5072-5076; Wyborski et al. (1991) Nucleic Acids Res. 19:4647-4653; Hillenand-Wissman (1989) Topics Mol. Struc. Biol. 10:143-162; Degenkolb et al. (1991) Antimicrob. Agents Chemother. 35:1591-1595; Kleinschnidt et al. (1988) Biochemistry 27:1094-1104; Bonin (1993) Ph.D. Thesis, University of Heidelberg; Gossen et al. (1992) Proc. Natl. Acad. Sci. USA 89:5547-5551; Oliva et al. (1992) Antimicrob. Agents Chemother. 36:913-919; Hlavka et al. (1985) Handbook of Experimental Pharmacology, Vol. 78 (Springer-Verlag, Berlin); Gill et al. (1988) Nature 334:721-724. The above list of selectable marker genes is not meant to be limiting. Any selectable marker gene can be used with the compositions and methods described herein.
VI. Compositions Comprising Silencing Elements
[0122] One or more of the polynucleotides comprising the silencing element may be provided as an external composition such as a spray or powder to the plant, plant part, seed, a plant insect pest, or an area of cultivation. In another example, a plant is transformed with a DNA construct or expression cassette for expression of at least one silencing element. In either composition, the silencing element, when ingested by an insect, can reduce the level of a target pest sequence and thereby control the pest (i.e., a Coleopteran plant pest including a Diabrotica plant pest, such as, D. virgifera virgifera, D. barberi, D. virgifera zeae, D. speciosa, or D. undecimpunctata howardi). It is recognized that the composition may comprise a cell (such as plant cell or a bacterial cell), in which a polynucleotide encoding the silencing element is stably incorporated into the genome and operably linked to promoters active in the cell. Compositions comprising a mixture of cells, some cells expressing at least one silencing element are also encompassed. In other embodiments, compositions comprising the silencing elements are not contained in a cell. In such embodiments, the composition can be applied to an area inhabited by a plant insect pest. In one embodiment, the composition is applied externally to a plant (i.e., by spraying a field or area of cultivation) to protect the plant from the pest. Methods of applying nucleotides in such a manner are known to those of skill in the art.
[0123] A composition disclosed herein may further be formulated as bait. In this embodiment, the compositions comprise a food substance or an attractant which enhances the attractiveness of the composition to the pest.
[0124] A composition comprising the silencing element may be formulated in an agriculturally suitable and/or environmentally acceptable carrier. Such carriers may be any material that the animal, plant or environment to be treated can tolerate. Furthermore, the carrier must be such that the composition remains effective at controlling a plant insect pest. Examples of such carriers include water, saline, Ringer's solution, dextrose or other sugar solutions, Hank's solution, and other aqueous physiologically balanced salt solutions, phosphate buffer, bicarbonate buffer and Tris buffer. In addition, the composition may include compounds that increase the half-life of a composition. Various insecticidal formulations can also be found in, for example, US Publications 2008/0275115, 2008/0242174, 2008/0027143, 2005/0042245, and 2004/0127520.
[0125] It is recognized that the polynucleotides comprising sequences encoding the silencing element may be used to transform organisms to provide for host organism production of these components, and subsequent application of the host organism to the environment of the target pest(s). Such host organisms include baculoviruses, bacteria, and the like. In this manner, the combination of polynucleotides encoding the silencing element may be introduced via a suitable vector into a microbial host, and said host applied to the environment, or to plants or animals.
[0126] The term "introduced" in the context of inserting a nucleic acid into a cell, means "transfection" or "transformation" or "transduction" and includes reference to the incorporation of a nucleic acid into a eukaryotic or prokaryotic cell where the nucleic acid may be stably incorporated into the genome of the cell (e.g., chromosome, plasmid, plastid, or mitochondrial DNA), converted into an autonomous replicon, or transiently expressed (e.g., transfected mRNA).
[0127] Microbial hosts that are known to occupy the "phytosphere" (phylloplane, phyllosphere, rhizosphere, and/or rhizoplana) of one or more crops of interest may be selected. These microorganisms are selected so as to be capable of successfully competing in the particular environment with the wild-type microorganisms, provide for stable maintenance and expression of the sequences encoding the silencing element, and desirably, provide for improved protection of the components from environmental degradation and inactivation.
[0128] Such microorganisms include bacteria, algae, and fungi. Of particular interest are microorganisms such as bacteria, e.g., Pseudomonas, Erwinia, Serratia, Klebsiella, Xanthomonas, Streptomyces, Rhizobium, Rhodopseudomonas, Methylius, Agrobacterium, Acetobacter, Lactobacillus, Arthrobacter, Azotobacter, Leuconostoc, and Alcaligenes, fungi, particularly yeast, e.g., Saccharomyces, Cryptococcus, Kluyveromyces, Sporobolomyces, Rhodotorula, and Aureobasidium. Of particular interest are such phytosphere bacterial species as Pseudomonas syringae, Pseudomonas fluorescens, Serratia marcescens, Acetobacter xylinum, Agro bacteria, Rhodopseudomonas spheroides, Xanthomonas campestris, Rhizobium melioti, Alcaligenes entrophus, Clavibacter xyli and Azotobacter vinlandir, and phytosphere yeast species such as Rhodotorula rubra, R. glutinis, R. marina, R. aurantiaca, Cryptococcus albidus, C. diffluens, C. laurentii, Saccharomyces rosei, S. pretoriensis, S. cerevisiae, Sporobolomyces rosues, S. odorus, Kluyveromyces veronae, and Aureobasidium pollulans. Of particular interest are the pigmented microorganisms.
[0129] A number of ways are available for introducing the polynucleotide comprising the silencing element into the microbial host under conditions that allow for stable maintenance and expression of such nucleotide encoding sequences. For example, expression cassettes can be constructed which include the nucleotide constructs of interest operably linked with the transcriptional and translational regulatory signals for expression of the nucleotide constructs, and a nucleotide sequence homologous with a sequence in the host organism, whereby integration will occur, and/or a replication system that is functional in the host, whereby integration or stable maintenance will occur.
[0130] Transcriptional and translational regulatory signals include, but are not limited to, promoters, transcriptional initiation start sites, operators, activators, enhancers, other regulatory elements, ribosomal binding sites, an initiation codon, termination signals, and the like. See, for example, U.S. Pat. Nos. 5,039,523 and 4,853,331; EP 0480762A2; Sambrook et al. (2000); Molecular Cloning: A Laboratory Manual (3.sup.rd edition; Cold Spring Harbor Laboratory Press, Plainview, N.Y.); Davis et al. (1980) Advanced Bacterial Genetics (Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.); and the references cited therein.
[0131] Suitable host cells include the prokaryotes and the lower eukaryotes, such as fungi. Illustrative prokaryotes, both Gram-negative and Gram-positive, include Enterobacteriaceae, such as Escherichia, Erwinia, Shigella, Salmonella, and Proteus; Bacillaceae; Rhizobiceae, such as Rhizobium; Spirillaceae, such as photobacterium, Zymomonas, Serratia, Aeromonas, Vibrio, Desulfovibrio, Spirillum; Lactobacillaceae; Pseudomonadaceae, such as Pseudomonas and Acetobacter; Azotobacteraceae and Nitrobacteraceae. Among eukaryotes are fungi, such as Phycomycetes and Ascomycetes, which includes yeast, such as Saccharomyces and Schizosaccharomyces; and Basidiomycetes yeast, such as Rhodotorula, Aureobasidium, Sporobolomyces, and the like.
[0132] Characteristics of particular interest in selecting a host cell may include ease of introducing the coding sequence into the host, availability of expression systems, efficiency of expression, stability in the host, and the presence of auxiliary genetic capabilities. Characteristics of interest for use as a pesticide microcapsule include protective qualities, such as thick cell walls, pigmentation, and intracellular packaging or formation of inclusion bodies; leaf affinity; lack of mammalian toxicity; attractiveness to pests for ingestion; and the like. Other considerations include ease of formulation and handling, economics, storage stability, and the like.
[0133] Host organisms of particular interest include yeast, such as Rhodotorula spp., Aureobasidium spp., Saccharomyces spp., and Sporobolomyces spp., phylloplane organisms such as Pseudomonas spp., Erwinia spp., and Flavobacterium spp., and other such organisms, including Pseudomonas aeruginosa, Pseudomonas fluorescens, Saccharomyces cerevisiae, Bacillus thuringiensis, Escherichia coli, Bacillus subtilis, and the like.
[0134] The sequences encoding the silencing elements encompassed by the invention may be introduced into microorganisms that multiply on plants (epiphytes) to deliver these components to potential target pests. Epiphytes, for example, can be gram-positive or gram-negative bacteria.
[0135] A silencing element may be fermented in a bacterial host and the resulting bacteria processed and used as a microbial spray in the same manner that Bacillus thuringiensis strains have been used as insecticidal sprays. Any suitable microorganism can be used for this purpose. By way of example, Pseudomonas has been used to express Bacillus thuringiensis endotoxins as encapsulated proteins and the resulting cells processed and sprayed as an insecticide Gaertner et al. (1993), in Advanced Engineered Pesticides, ed. L. Kim (Marcel Decker, Inc.).
[0136] Alternatively, the components of the invention are produced by introducing heterologous genes into a cellular host. Expression of the heterologous sequences results, directly or indirectly, in the intracellular production of a silencing element. These compositions may then be formulated in accordance with conventional techniques for application to the environment hosting a target pest, e.g., soil, water, and foliage of plants. See, for example, EPA 0192319, and the references cited therein.
[0137] A transformed microorganism can be formulated with an acceptable carrier into separate or combined compositions that are, for example, a suspension, a solution, an emulsion, a dusting powder, a dispersible granule, a wettable powder, and an emulsifiable concentrate, an aerosol, an impregnated granule, an adjuvant, a coatable paste, and also encapsulations in, for example, polymer substances.
[0138] Such compositions disclosed above may be obtained by the addition of a surface-active agent, an inert carrier, a preservative, a humectant, a feeding stimulant, an attractant, an encapsulating agent, a binder, an emulsifier, a dye, a UV protectant, a buffer, a flow agent or fertilizers, micronutrient donors, or other preparations that influence plant growth. One or more agrochemicals including, but not limited to, herbicides, insecticides, fungicides, bactericides, nematicides, molluscicides, acaracides, plant growth regulators, harvest aids, and fertilizers, can be combined with carriers, surfactants or adjuvants customarily employed in the art of formulation or other components to facilitate product handling and application for particular target pests. Suitable carriers and adjuvants can be solid or liquid and correspond to the substances ordinarily employed in formulation technology, e.g., natural or regenerated mineral substances, solvents, dispersants, wetting agents, tackifiers, binders, or fertilizers. The active ingredients (i.e., at least one silencing element) are normally applied in the form of compositions and can be applied to the crop area, plant, or seed to be treated. For example, the compositions may be applied to grain in preparation for or during storage in a grain bin or silo, etc. The compositions may be applied simultaneously or in succession with other compounds. Methods of applying an active ingredient or a composition that contains at least one silencing element include, but are not limited to, foliar application, seed coating, and soil application. The number of applications and the rate of application depend on the intensity of infestation by the corresponding pest.
[0139] Suitable surface-active agents include, but are not limited to, anionic compounds such as a carboxylate of, for example, a metal; carboxylate of a long chain fatty acid; an N-acylsarcosinate; mono- or di-esters of phosphoric acid with fatty alcohol ethoxylates or salts of such esters; fatty alcohol sulfates such as sodium dodecyl sulfate, sodium octadecyl sulfate, or sodium cetyl sulfate; ethoxylated fatty alcohol sulfates; ethoxylated alkylphenol sulfates; lignin sulfonates; petroleum sulfonates; alkyl aryl sulfonates such as alkyl-benzene sulfonates or lower alkylnaphtalene sulfonates, e.g., butyl-naphthalene sulfonate; salts of sulfonated naphthalene-formaldehyde condensates; salts of sulfonated phenol-formaldehyde condensates; more complex sulfonates such as the amide sulfonates, e.g., the sulfonated condensation product of oleic acid and N-methyl taurine; or the dialkyl sulfosuccinates, e.g., the sodium sulfonate or dioctyl succinate. Non-ionic agents include condensation products of fatty acid esters, fatty alcohols, fatty acid amides or fatty-alkyl- or alkenyl-substituted phenols with ethylene oxide, fatty esters of polyhydric alcohol ethers, e.g., sorbitan fatty acid esters, condensation products of such esters with ethylene oxide, e.g., polyoxyethylene sorbitan fatty acid esters, block copolymers of ethylene oxide and propylene oxide, acetylenic glycols such as 2,4,7,9-tetraethyl-5-decyn-4,7-diol, or ethoxylated acetylenic glycols. Examples of a cationic surface-active agent include, for instance, an aliphatic mono-, di-, or polyamine such as an acetate, naphthenate or oleate; or oxygen-containing amine such as an amine oxide of polyoxyethylene alkylamine; an amide-linked amine prepared by the condensation of a carboxylic acid with a di- or polyamine; or a quaternary ammonium salt.
[0140] Examples of inert materials include, but are not limited to, inorganic minerals such as kaolin, phyllosilicates, carbonates, sulfates, phosphates, or botanical materials such as cork, powdered corncobs, peanut hulls, rice hulls, and walnut shells.
[0141] The compositions comprising a silencing element may be in a suitable form for direct application or as a concentrate of primary composition that requires dilution with a suitable quantity of water or other dilutant before application.
[0142] The compositions (including the transformed microorganisms) may be applied to the environment of an insect pest (such as a Coleoptera plant pest or a Diabrotica plant pest) by, for example, spraying, atomizing, dusting, scattering, coating or pouring, introducing into or on the soil, introducing into irrigation water, by seed treatment or general application or dusting at the time when the pest has begun to appear or before the appearance of pests as a protective measure. For example, the composition(s) and/or transformed microorganism(s) may be mixed with grain to protect the grain during storage. It is generally important to obtain good control of pests in the early stages of plant growth, as this is the time when the plant can be most severely damaged. The compositions can conveniently contain another insecticide if this is thought necessary. In an embodiment of the invention, the composition(s) is applied directly to the soil, at a time of planting, in granular form of a composition of a carrier and dead cells of a Bacillus strain or transformed microorganism of the invention. Another embodiment is a granular form of a composition comprising an agrochemical such as, for example, an herbicide, an insecticide, a fertilizer, in an inert carrier, and dead cells of a Bacillus strain or transformed microorganism of the invention.
VII. Plants, Plant Parts, and Methods of Introducing Sequences into Plants
[0143] In one embodiment, the methods of the invention involve introducing a polynucleotide into a plant. "Introducing" is intended to mean presenting to the plant the polynucleotide in such a manner that the sequence gains access to the interior of a cell of the plant. The methods of the invention do not depend on a particular method for introducing a sequence into a plant, only that the polynucleotide or polypeptides gains access to the interior of at least one cell of the plant. Methods for introducing polynucleotides into plants are known in the art including, but not limited to, stable transformation methods, transient transformation methods, and virus-mediated methods.
[0144] "Stable transformation" is intended to mean that the nucleotide construct introduced into a plant integrates into the genome of the plant and is capable of being inherited by the progeny thereof. "Transient transformation" is intended to mean that a polynucleotide is introduced into the plant and does not integrate into the genome of the plant or a polypeptide is introduced into a plant.
[0145] Transformation protocols as well as protocols for introducing polypeptides or polynucleotide sequences into plants may vary depending on the type of plant or plant cell, i.e., monocot or dicot, targeted for transformation. Suitable methods of introducing polypeptides and polynucleotides into plant cells include microinjection (Crossway et al. (1986) Biotechniques 4:320-334), electroporation (Riggs et al. (1986) Proc. Natl. Acad. Sci. USA 83:5602-5606, Agrobacterium-mediated transformation (U.S. Pat. No. 5,563,055 and U.S. Pat. No. 5,981,840), direct gene transfer (Paszkowski et al. (1984) EMBO J. 3:2717-2722), and ballistic particle acceleration (see, for example, U.S. Pat. No. 4,945,050; U.S. Pat. No. 5,879,918; U.S. Pat. Nos. 5,886,244; and, 5,932,782; Tomes et al. (1995) in Plant Cell, Tissue, and Organ Culture: Fundamental Methods, ed. Gamborg and Phillips (Springer-Verlag, Berlin); McCabe et al. (1988) Biotechnology 6:923-926); and Lec1 transformation (WO 00/28058). Also see Weissinger et al. (1988) Ann. Rev. Genet. 22:421-477; Sanford et al. (1987) Particulate Science and Technology 5:27-37 (onion); Christou et al. (1988) Plant Physiol. 87:671-674 (soybean); McCabe et al. (1988) Bio/Technology 6:923-926 (soybean); Finer and McMullen (1991) In Vitro Cell Dev. Biol. 27P:175-182 (soybean); Singh et al. (1998) Theor. Appl. Genet. 96:319-324 (soybean); Datta et al. (1990) Biotechnology 8:736-740 (rice); Klein et al. (1988) Proc. Natl. Acad. Sci. USA 85:4305-4309 (maize); Klein et al. (1988) Biotechnology 6:559-563 (maize); U.S. Pat. Nos. 5,240,855; 5,322,783; and, 5,324,646; Klein et al. (1988) Plant Physiol. 91:440-444 (maize); Fromm et al. (1990) Biotechnology 8:833-839 (maize); Hooykaas-Van Slogteren et al. (1984) Nature (London) 311:763-764; U.S. Pat. No. 5,736,369 (cereals); Bytebier et al. (1987) Proc. Natl. Acad. Sci. USA 84:5345-5349 (Liliaceae); De Wet et al. (1985) in The Experimental Manipulation of Ovule Tissues, ed. Chapman et al. (Longman, New York), pp. 197-209 (pollen); Kaeppler et al. (1990) Plant Cell Reports 9:415-418 and Kaeppler et al. (1992) Theor. Appl. Genet. 84:560-566 (whisker-mediated transformation); D'Halluin et al. (1992) Plant Cell 4:1495-1505 (electroporation); Li et al. (1993) Plant Cell Reports 12:250-255 and Christou and Ford (1995) Annals of Botany 75:407-413 (rice); Osjoda et al. (1996) Nature Biotechnology 14:745-750 (maize via Agrobacterium tumefaciens).
[0146] In certain embodiments, a silencing element disclosed herein may be provided to a plant using a variety of transient transformation methods. Such transient transformation methods include, but are not limited to, the introduction of the protein or variants or fragments thereof directly into the plant or the introduction of the transcript into the plant. Such methods include, for example, microinjection or particle bombardment. See, for example, Crossway et al. (1986) Mol Gen. Genet. 202:179-185; Nomura et al. (1986) Plant Sci. 44:53-58; Hepler et al. (1994) Proc. Natl. Acad. Sci. 91: 2176-2180 and Hush et al. (1994) The Journal of Cell Science 107:775-784, all of which are herein incorporated by reference. Alternatively, polynucleotides can be transiently transformed into the plant using techniques known in the art. Such techniques include viral vector systems and the precipitation of the polynucleotide in a manner that precludes subsequent release of the DNA. Such methods include the use of particles coated with polyethylimine (PEI; Sigma #P3143).
[0147] In other embodiments, the polynucleotides disclosed herein may be introduced into plants by contacting plants with a virus or viral nucleic acids. Generally, such methods involve incorporating a nucleotide construct of the invention within a viral DNA or RNA molecule. Further, it is recognized that promoters may also encompass promoters utilized for transcription by viral RNA polymerases. Methods for introducing polynucleotides into plants and expressing a protein encoded therein, involving viral DNA or RNA molecules, are known in the art. See, for example, U.S. Pat. Nos. 5,889,191, 5,889,190, 5,866,785, 5,589,367, 5,316,931, and Porta et al. (1996) Molecular Biotechnology 5:209-221.
[0148] Methods are known in the art for the targeted insertion of a polynucleotide at a specific location in the plant genome. In one embodiment, the insertion of the polynucleotide at a desired genomic location is achieved using a site-specific recombination system. See, for example, WO99/25821, WO99/25854, WO99/25840, WO99/25855, and WO99/25853. Briefly, the polynucleotides disclosed herein may be contained in transfer cassette flanked by two non-recombinogenic recombination sites. The transfer cassette is introduced into a plant having stably incorporated into its genome a target site which is flanked by two non-recombinogenic recombination sites that correspond to the sites of the transfer cassette. An appropriate recombinase is provided and the transfer cassette is integrated at the target site. The polynucleotide of interest is thereby integrated at a specific chromosomal position in the plant genome.
[0149] The cells that have been transformed may be grown into plants in accordance with conventional ways. See, for example, McCormick et al. (1986) Plant Cell Reports 5:81-84. These plants may then be grown, and either pollinated with the same transformed strain or different strains, and the resulting progeny having constitutive expression of the desired phenotypic characteristic identified. Two or more generations may be grown to ensure that expression of the desired phenotypic characteristic is stably maintained and inherited and then seeds harvested to ensure expression of the desired phenotypic characteristic has been achieved. In this manner, the compositions and methods described herein provide transformed seeds (also referred to as "transgenic seed") having a polynucleotide disclosed herein, for example, an expression cassette, stably incorporated into their genome.
[0150] As used herein, the term plant includes plant cells, plant protoplasts, plant cell tissue cultures from which plants can be regenerated, plant calli, plant clumps, and plant cells that are intact in plants or parts of plants such as embryos, pollen, ovules, seeds, leaves, flowers, branches, fruit, kernels, ears, cobs, husks, stalks, roots, root tips, anthers, and the like. Grain is intended to mean the mature seed produced by commercial growers for purposes other than growing or reproducing the species. Progeny, variants, and mutants of the regenerated plants are also included within the scope of the invention, provided that these parts comprise the introduced polynucleotides.
[0151] The compositions and methods described herein may be used for transformation of any plant species, including, but not limited to, monocots and dicots. Examples of plant species of interest include, but are not limited to, corn (Zea mays), Brassica sp. (e.g., B. napus, B. rapa, B. juncea), particularly those Brassica species useful as sources of seed oil, alfalfa (Medicago sativa), rice (Oryza sativa), rye (Secale cereale), sorghum (Sorghum bicolor, Sorghum vulgare), millet (e.g., pearl millet (Pennisetum glaucum), proso millet (Panicum miliaceum), foxtail millet (Setaria italica), finger millet (Eleusine coracana)), sunflower (Helianthus annuus), safflower (Carthamus tinctorius), wheat (Triticum aestivum), soybean (Glycine max), tobacco (Nicotiana tabacum), potato (Solanum tuberosum), peanuts (Arachis hypogaea), cotton (Gossypium barbadense, Gossypium hirsutum), sweet potato (Ipomoea batatus), cassava (Manihot esculenta), coffee (Coffea spp.), coconut (Cocos nucifera), pineapple (Ananas comosus), citrus trees (Citrus spp.), cocoa (Theobroma cacao), tea (Camellia sinensis), banana (Musa spp.), avocado (Persea americana), fig (Ficus casica), guava (Psidium guajava), mango (Mangifera indica), olive (Olea europaea), papaya (Carica papaya), cashew (Anacardium occidentale), macadamia (Macadamia integrifolia), almond (Prunus amygdalus), sugar beets (Beta vulgaris), sugarcane (Saccharum spp.), oats, barley, vegetables, ornamentals, and conifers.
[0152] Vegetables include tomatoes (Lycopersicon esculentum), lettuce (e.g., Lactuca sativa), green beans (Phaseolus vulgaris), lima beans (Phaseolus limensis), peas (Lathyrus spp.), and members of the genus Cucumis such as cucumber (C. sativus), cantaloupe (C. cantalupensis), and musk melon (C. melo). Ornamentals include azalea (Rhododendron spp.), hydrangea (Macrophylla hydrangea), hibiscus (Hibiscus rosasanensis), roses (Rosa spp.), tulips (Tulipa spp.), daffodils (Narcissus spp.), petunias (Petunia hybrida), carnation (Dianthus caryophyllus), poinsettia (Euphorbia pulcherrima), and chrysanthemum.
[0153] Conifers that may be employed in practicing the compositions and methods described herein include, for example, pines such as loblolly pine (Pinus taeda), slash pine (Pinus elliotii), ponderosa pine (Pinus ponderosa), lodgepole pine (Pinus contorta), and Monterey pine (Pinus radiata); Douglas-fir (Pseudotsuga menziesii); Western hemlock (Tsuga canadensis); Sitka spruce (Picea glauca); redwood (Sequoia sempervirens); true firs such as silver fir (Abies amabilis) and balsam fir (Abies balsamea); and cedars such as Western red cedar (Thuja plicata) and Alaska yellow-cedar (Chamaecyparis nootkatensis). In certain embodiments, the compositions and methods described herein can be used with plants such as crop plants (for example, corn, alfalfa, sunflower, Brassica, soybean, cotton, safflower, peanut, sorghum, wheat, millet, tobacco, etc.). In other embodiments, corn and soybean plants and sugarcane plants are optimal, and in yet other embodiments corn plants are optimal.
[0154] Other plants of interest include grain plants that provide seeds of interest, oil-seed plants, and leguminous plants. Seeds of interest include grain seeds, such as corn, wheat, barley, rice, sorghum, rye, etc. Oil-seed plants include cotton, soybean, safflower, sunflower, Brassica, maize, alfalfa, palm, coconut, etc. Leguminous plants include beans and peas. Beans include guar, locust bean, fenugreek, soybean, garden beans, cowpea, mungbean, lima bean, fava bean, lentils, chickpea, etc.
VIII. Stacking of Traits in Transgenic Plant
[0155] Transgenic plants may comprise a stack of one or more target polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or variants or fragments thereof, or complements thereof, as disclosed herein with one or more additional polynucleotides resulting in the production or suppression of multiple polypeptide sequences. Transgenic plants comprising stacks of polynucleotide sequences can be obtained by either or both of traditional breeding methods or through genetic engineering methods. These methods include, but are not limited to, breeding individual lines each comprising a polynucleotide of interest, transforming a transgenic plant comprising an expression construct comprising various target polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or encoding silencing elements directed to such target sequence variants or fragments thereof, or complements thereof, as disclosed herein with a subsequent gene and co-transformation of genes into a single plant cell. As used herein, the term "stacked" includes having the multiple traits present in the same plant (i.e., both traits are incorporated into the nuclear genome, one trait is incorporated into the nuclear genome and one trait is incorporated into the genome of a plastid or both traits are incorporated into the genome of a plastid). In one non-limiting example, "stacked traits" comprise a molecular stack where the sequences are physically adjacent to each other. A trait, as used herein, refers to the phenotype derived from a particular sequence or groups of sequences. Co-transformation of polynucleotides can be carried out using single transformation vectors comprising multiple polynucleotides or polynucleotides carried separately on multiple vectors. If the sequences are stacked by genetically transforming the plants, the polynucleotide sequences of interest can be combined at any time and in any order. The traits can be introduced simultaneously in a co-transformation protocol with the polynucleotides of interest provided by any combination of transformation cassettes. For example, if two sequences will be introduced, the two sequences can be contained in separate transformation cassettes (trans) or contained on the same transformation cassette (cis). Expression of the sequences can be driven by the same promoter or by different promoters. It is further recognized that polynucleotide sequences can be stacked at a desired genomic location using a site-specific recombination system. See, for example, WO 1999/25821, WO 1999/25854, WO 1999/25840, WO 1999/25855 and WO 1999/25853.
[0156] In some embodiments the various target polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, silencing elements directed to such target sequences, and variants or fragments thereof, or complements thereof, as disclosed herein, alone or stacked with one or more additional insect resistance traits can be stacked with one or more additional input traits (e.g., herbicide resistance, fungal resistance, virus resistance, stress tolerance, disease resistance, male sterility, stalk strength, and the like) or output traits (e.g., increased yield, modified starches, improved oil profile, balanced amino acids, high lysine or methionine, increased digestibility, improved fiber quality, drought resistance, and the like). Thus, the polynucleotide embodiments can be used to provide a complete agronomic package of improved crop quality with the ability to flexibly and cost effectively control any number of agronomic pests.
[0157] Transgenes useful for stacking include, but are not limited to, to those as described herein below.
[0158] i. Transgenes that Confer Resistance to Insects or Disease
[0159] (A) Plant disease resistance genes. Plant defenses are often activated by specific interaction between the product of a disease resistance gene (R) in the plant and the product of a corresponding avirulence (Avr) gene in the pathogen. A plant variety can be transformed with cloned resistance gene to engineer plants that are resistant to specific pathogen strains. See, for example, Jones, et al., (1994) Science 266:789 (cloning of the tomato Cf-9 gene for resistance to Cladosporium fulvum); Martin, et al., (1993) Science 262:1432 (tomato Pto gene for resistance to Pseudomonas syringae pv. tomato encodes a protein kinase); Mindrinos, et al., (1994) Cell 78:1089 (Arabidopsis RSP2 gene for resistance to Pseudomonas syringae), McDowell and Woffenden, (2003) Trends Biotechnol. 21(4):178-83 and Toyoda, et al., (2002) Transgenic Res. 11(6):567-82. A plant resistant to a disease is one that is more resistant to a pathogen as compared to the wild type plant.
[0160] (B) Genes encoding a Bacillus thuringiensis protein, a derivative thereof or a synthetic polypeptide modeled thereon. See, for example, Geiser, et al., (1986) Gene 48:109, who disclose the cloning and nucleotide sequence of a Bt delta-endotoxin gene. Moreover, DNA molecules encoding delta-endotoxin genes can be purchased from American Type Culture Collection (Rockville, Md.), for example, under ATCC.RTM. Accession Numbers 40098, 67136, 31995 and 31998. Other non-limiting examples of Bacillus thuringiensis transgenes being genetically engineered are given in the following patents and patent applications and hereby are incorporated by reference for this purpose: U.S. Pat. Nos. 5,188,960; 5,689,052; 5,880,275; 5,986,177; 6,023,013, 6,060,594, 6,063,597, 6,077,824, 6,620,988, 6,642,030, 6,713,259, 6,893,826, 7,105,332; 7,179,965, 7,208,474; 7,227,056, 7,288,643, 7,323,556, 7,329,736, 7,449,552, 7,468,278, 7,510,878, 7,521,235, 7,544,862, 7,605,304, 7,696,412, 7,629,504, 7,705,216, 7,772,465, 7,790,846, 7,858,849 and WO 1991/14778; WO 1999/31248; WO 2001/12731; WO 1999/24581 and WO 1997/40162.
[0161] Genes encoding pesticidal proteins may also be stacked including but are not limited to: insecticidal proteins from Pseudomonas sp. such as PSEEN3174 (Monalysin, (2011) PLoS Pathogens, 7:1-13), from Pseudomonas protegens strain CHAO and Pf-5 (previously fluorescens) (Pechy-Tarr, (2008) Environmental Microbiology 10:2368-2386: GenBank Accession No. EU400157); from Pseudomonas Taiwanensis (Liu, et al., (2010) J. Agric. Food Chem. 58:12343-12349) and from Pseudomonas pseudoalcligenes (Zhang, et al., (2009) Annals of Microbiology 59:45-50 and Li, et al., (2007) Plant Cell Tiss. Organ Cult. 89:159-168); insecticidal proteins from Photorhabdus sp. and Xenorhabdus sp. (Hinchliffe, et al., (2010) The Open Toxinology Journal 3:101-118 and Morgan, et al., (2001) Applied and Envir. Micro. 67:2062-2069), U.S. Pat. No. 6,048,838, and U.S. Pat. No. 6,379,946; a PIP-1 polypeptide of US Patent Publication US20140007292; an AfIP-1A and/or AfIP-1B polypeptide of US Patent Publication US20140033361; a PHI-4 polypeptide of US Patent Publication US20140274885 and US20160040184; a PIP-47 polypeptide of PCT Publication Number WO2015/023846, a PIP-72 polypeptide of PCT Publication Number WO2015/038734; a PtIP-50 polypeptide and a PtIP-65 polypeptide of PCT Publication Number WO2015/120270; a PtIP-83 polypeptide of PCT Publication Number WO2015/120276; a PtIP-96 polypeptide of PCT Serial Number PCT/US15/55502; and .delta.-endotoxins including, but not limited to, the Cry1, Cry2, Cry3, Cry4, Cry5, Cry6, Cry7, Cry8, Cry9, Cry10, Cry11, Cry12, Cry13, Cry14, Cry15, Cry16, Cry17, Cry18, Cry19, Cry20, Cry21, Cry22, Cry23, Cry24, Cry25, Cry26, Cry27, Cry 28, Cry 29, Cry 30, Cry31, Cry32, Cry33, Cry34, Cry35, Cry36, Cry37, Cry38, Cry39, Cry40, Cry41, Cry42, Cry43, Cry44, Cry45, Cry 46, Cry47, Cry49, Cry 51 and Cry55 classes of .delta.-endotoxin genes and the B. thuringiensis cytolytic Cyt1 and Cyt2 genes. Members of these classes of B. thuringiensis insecticidal proteins include, but are not limited to Cry1Aa1 (Accession # AAA22353); Cry1Aa2 (Accession # Accession # AAA22552); Cry1Aa3 (Accession # BAA00257); Cry1Aa4 (Accession # CAA31886); Cry1Aa5 (Accession # BAA04468); Cry1Aa6 (Accession # AAA86265); Cry1Aa7 (Accession # AAD46139); Cry1Aa8 (Accession #126149); Cry1Aa9 (Accession # BAA77213); Cry1Aa10 (Accession # AAD55382); Cry1Aa11 (Accession # CAA70856); Cry1Aa12 (Accession # AAP80146); Cry1Aa13 (Accession # AAM44305); Cry1Aa14 (Accession # AAP40639); Cry1Aa15 (Accession # AAY66993); Cry1Aa16 (Accession # HQ439776); Cry1Aa17 (Accession # HQ439788); Cry1Aa18 (Accession # HQ439790); Cry1Aa19 (Accession # HQ685121); Cry1Aa20 (Accession # JF340156); Cry1Aa21 (Accession # JN651496); Cry1Aa22 (Accession # KC158223); Cry1Ab1 (Accession # AAA22330); Cry1Ab2 (Accession # AAA22613); Cry1Ab3 (Accession # AAA22561); Cry1Ab4 (Accession # BAA00071); Cry1Ab5 (Accession # CAA28405); Cry1Ab6 (Accession # AAA22420); Cry1Ab7 (Accession # CAA31620); Cry1Ab8 (Accession # AAA22551); Cry1Ab9 (Accession # CAA38701); Cry1Ab10 (Accession # A29125); Cry1Ab11 (Accession #112419); Cry1Ab12 (Accession # AAC64003); Cry1Ab13 (Accession # AAN76494); Cry1Ab14 (Accession # AAG16877); Cry1Ab15 (Accession # AAO13302); Cry1Ab16 (Accession # AAK55546); Cry1Ab17 (Accession # AAT46415); Cry1Ab18 (Accession # AAQ88259); Cry1Ab19 (Accession # AAW31761); Cry1Ab20 (Accession # ABB72460); Cry1Ab21 (Accession # ABS18384); Cry1Ab22 (Accession # ABW87320); Cry1Ab23 (Accession # HQ439777); Cry1Ab24 (Accession # HQ439778); Cry1Ab25 (Accession # HQ685122); Cry1Ab26 (Accession # HQ847729); Cry1Ab27 (Accession # JN135249); Cry1Ab28 (Accession # JN135250); Cry1Ab29 (Accession # JN135251); Cry1Ab30 (Accession # JN135252); Cry1Ab31 (Accession # JN135253); Cry1Ab32 (Accession # JN135254); Cry1Ab33 (Accession # AAS93798); Cry1Ab34 (Accession # KC156668); Cry1Ab-like (Accession # AAK14336); Cry1Ab-like (Accession # AAK14337); Cry1Ab-like (Accession # AAK14338); Cry1Ab-like (Accession # ABG88858); Cry1Ac1 (Accession # AAA22331); Cry1Ac2 (Accession # AAA22338); Cry1Ac3 (Accession # CAA38098); Cry1Ac4 (Accession # AAA73077); Cry1Ac5 (Accession # AAA22339); Cry1Ac6 (Accession # AAA86266); Cry1Ac7 (Accession # AAB46989); Cry1Ac8 (Accession # AAC44841); Cry1Ac9 (Accession # AAB49768); Cry1Ac10 (Accession # CAA05505); Cry1Ac11 (Accession # CAA10270); Cry1Ac12 (Accession #112418); Cry1Ac13 (Accession # AAD38701); Cry1Ac14 (Accession # AAQ06607); Cry1Ac15 (Accession # AAN07788); Cry1Ac16 (Accession # AAU87037); Cry1Ac17 (Accession # AAX18704); Cry1Ac18 (Accession # AAY88347); Cry1Ac19 (Accession # ABD37053); Cry1Ac20 (Accession # ABB89046); Cry1Ac21 (Accession # AAY66992); Cry1Ac22 (Accession # ABZ01836); Cry1Ac23 (Accession # CAQ30431); Cry1Ac24 (Accession # ABL01535); Cry1Ac25 (Accession # FJ513324); Cry1Ac26 (Accession # FJ617446); Cry1Ac27 (Accession # FJ617447); Cry1Ac28 (Accession # ACM90319); Cry1Ac29 (Accession # DQ438941); Cry1Ac30 (Accession # GQ227507); Cry1Ac31 (Accession # GU446674); Cry1Ac32 (Accession # HM061081); Cry1Ac33 (Accession # GQ866913); Cry1Ac34 (Accession # HQ230364); Cry1Ac35 (Accession # JF340157); Cry1Ac36 (Accession # JN387137); Cry1Ac37 (Accession # JQ317685); Cry1Ad1 (Accession # AAA22340); Cry1Ad2 (Accession # CAA01880); Cry1Ae1 (Accession # AAA22410); Cry1Af1 (Accession # AAB82749); Cry1Ag1 (Accession # AAD46137); Cry1Ah1 (Accession # AAQ14326); Cry1Ah2 (Accession # ABB76664); Cry1Ah3 (Accession # HQ439779); Cry1Ai1 (Accession # AA039719); Cry1Ai2 (Accession # HQ439780); Cry1A-like (Accession # AAK14339); Cry1Ba1 (Accession # CAA29898); Cry1Ba2 (Accession # CAA65003); Cry1Ba3 (Accession # AAK63251); Cry1Ba4 (Accession # AAK51084); Cry1Ba5 (Accession # ABO20894); Cry1Ba6 (Accession # ABL60921); Cry1Ba7 (Accession # HQ439781); Cry1Bb1 (Accession # AAA22344); Cry1Bb2 (Accession # HQ439782); Cry1Bc1 (Accession # CAA86568); Cry1Bd1 (Accession # AAD10292); Cry1Bd2 (Accession # AAM93496); Cry1Be1 (Accession # AAC32850); Cry1Be2 (Accession # AAQ52387); Cry1Be3 (Accession # ACV96720); Cry1Be4 (Accession # HM070026); Cry1Bf1 (Accession # CAC50778); Cry1Bf2 (Accession # AAQ52380); Cry1Bg1 (Accession # AA039720); Cry1Bh1 (Accession # HQ589331); Cry1Bi1 (Accession # KC156700); Cry1Ca1 (Accession # CAA30396); Cry1Ca2 (Accession # CAA31951); Cry1Ca3 (Accession # AAA22343); Cry1Ca4 (Accession # CAA01886); Cry1Ca5 (Accession # CAA65457); Cry1Ca6 [1] (Accession # AAF37224); Cry1Ca7 (Accession # AAG50438); Cry1Ca8 (Accession # AAM00264); Cry1Ca9 (Accession # AAL79362); Cry1Ca10 (Accession # AAN16462); Cry1Ca11 (Accession # AAX53094); Cry1Ca12 (Accession # HM070027); Cry1Ca13 (Accession # HQ412621); Cry1Ca14 (Accession # JN651493); Cry1Cb1 (Accession # M97880); Cry1Cb2 (Accession # AAG35409); Cry1Cb3 (Accession # ACD50894); Cry1Cb-like (Accession # AAX63901); Cry1Da1 (Accession # CAA38099); Cry1Da2 (Accession #176415); Cry1Da3 (Accession # HQ439784); Cry1Db1 (Accession # CAA80234); Cry1Db2 (Accession # AAK48937); Cry1Dc1 (Accession # ABK35074); Cry1Ea1 (Accession # CAA37933); Cry1Ea2 (Accession # CAA39609); Cry1Ea3 (Accession # AAA22345); Cry1Ea4 (Accession # AAD04732); Cry1Ea5 (Accession # A15535); Cry1Ea6 (Accession # AAL50330); Cry1Ea7 (Accession # AAW72936); Cry1Ea8 (Accession # ABX11258); Cry1Ea9 (Accession # HQ439785); Cry1Ea10 (Accession # ADR00398); Cry1Ea11 (Accession # JQ652456); Cry1Eb1 (Accession # AAA22346); Cry1Fa1 (Accession # AAA22348); Cry1Fa2 (Accession # AAA22347); Cry1Fa3 (Accession # HM070028); Cry1Fa4 (Accession # HM439638); Cry1Fb1 (Accession # CAA80235); Cry1Fb2 (Accession # BAA25298); Cry1Fb3 (Accession # AAF21767); Cry1Fb4 (Accession # AAC10641); Cry1Fb5 (Accession # AA013295); Cry1Fb6 (Accession # ACD50892); Cry1Fb7 (Accession # ACD50893); Cry1Ga1 (Accession # CAA80233); Cry1Ga2 (Accession # CAA70506); Cry1Gb1 (Accession # AAD10291); Cry1Gb2 (Accession # AA013756); Cry1Gc1 (Accession # AAQ52381); Cry1Ha1 (Accession # CAA80236); Cry1Hb1 (Accession # AAA79694); Cry1Hb2 (Accession # HQ439786); Cry1H-like (Accession # AAF01213); Cry1Ia1 (Accession # CAA44633); Cry1Ia2 (Accession # AAA22354); Cry1Ia3 (Accession # AAC36999); Cry1Ia4 (Accession # AAB00958); Cry1Ia5 (Accession # CAA70124); Cry1Ia6 (Accession # AAC26910); Cry1Ia7 (Accession # AAM73516); Cry1Ia8 (Accession # AAK66742); Cry1Ia9 (Accession # AAQ08616); Cry1Ia10 (Accession # AAP86782); Cry1Ia11 (Accession # CAC85964); Cry1Ia12 (Accession # AAV53390); Cry1Ia13 (Accession # ABF83202); Cry1Ia14 (Accession # ACG63871); Cry1Ia15 (Accession # FJ617445); Cry1Ia16 (Accession # FJ617448); Cry1Ia17 (Accession # GU989199); Cry1Ia18 (Accession # ADK23801); Cry1Ia19 (Accession # HQ439787); Cry1Ia20 (Accession # JQ228426); Cry1Ia2l (Accession # JQ228424); Cry1Ia22 (Accession # JQ228427); Cry1Ia23 (Accession # JQ228428); Cry1Ia24 (Accession # JQ228429); Cry1Ia25 (Accession # JQ228430); Cry1Ia26 (Accession # JQ228431); Cry1Ia27 (Accession # JQ228432); Cry1Ia28 (Accession # JQ228433); Cry1Ia29 (Accession # JQ228434); Cry1Ia30 (Accession # JQ317686); Cry1Ia3l (Accession # JX944038); Cry1Ia32 (Accession # JX944039); Cry1Ia33 (Accession # JX944040); Cry1Ib1 (Accession # AAA82114); Cry1Ib2 (Accession # ABW88019); Cry1Ib3 (Accession # ACD75515); Cry1Ib4 (Accession # HM051227); Cry1Ib5 (Accession # HM070028); Cry1Ib6 (Accession # ADK38579); Cry1Ib7 (Accession # JN571740); Cry1Ib8 (Accession # JN675714); Cry1Ib9 (Accession # JN675715); Cry1Ib10 (Accession # JN675716); Cry1Ib11 (Accession # JQ228423); Cry1Ic1 (Accession # AAC62933); Cry1Ic2 (Accession # AAE71691); Cry1Id1 (Accession # AAD44366); Cry1Id2 (Accession # JQ228422); Cry1Ie1 (Accession # AAG43526); Cry1Ie2 (Accession # HM439636); Cry1Ie3 (Accession # KC156647); Cry1Ie4 (Accession # KCI56681); Cry1If1 (Accession # AAQ52382); Cry1Ig1 (Accession # KC156701); Cry1I-like (Accession # AAC31094); Cry1I-like (Accession # ABG88859); Cry1Ja1 (Accession # AAA22341); Cry1Ja2 (Accession # HM070030); Cry1Ja3 (Accession # JQ228425); Cry1Jb1 (Accession # AAA98959); Cry1Jc1 (Accession # AAC31092); Cry1Jc2 (Accession # AAQ52372); Cry1Jd1 (Accession # CAC50779); Cry1Ka1 (Accession # AAB00376); Cry1Ka2 (Accession # HQ439783); Cry1La1 (Accession # AAS60191); Cry1La2 (Accession # HM070031); Cry1Ma1 (Accession # FJ884067); Cry1Ma2 (Accession # KC156659); Cry1Na1 (Accession # KC156648); Cry1Nb1 (Accession # KC156678); Cry1-like (Accession # AAC31091); Cry2Aa1 (Accession # AAA22335); Cry2Aa2 (Accession # AAA83516); Cry2Aa3 (Accession # D86064); Cry2Aa4 (Accession # AAC04867); Cry2Aa5 (Accession # CAA10671); Cry2Aa6 (Accession # CAA10672); Cry2Aa7 (Accession # CAA10670); Cry2Aa8 (Accession # AA013734); Cry2Aa9 (Accession # AA013750); Cry2Aa10 (Accession # AAQ04263); Cry2Aa11 (Accession # AAQ52384); Cry2Aa12 (Accession # ABI83671); Cry2Aa13 (Accession # ABL01536); Cry2Aa14 (Accession # ACF04939); Cry2Aa15 (Accession # JN426947); Cry2Ab1 (Accession # AAA22342); Cry2Ab2 (Accession # CAA39075); Cry2Ab3 (Accession # AAG36762); Cry2Ab4 (Accession # AA013296); Cry2Ab5 (Accession # AAQ04609); Cry2Ab6 (Accession # AAP59457); Cry2Ab7 (Accession # AAZ66347); Cry2Ab8 (Accession # ABC95996); Cry2Ab9 (Accession # ABC74968); Cry2Ab10 (Accession # EF157306); Cry2Ab11 (Accession # CAM84575); Cry2Ab12 (Accession # ABM21764); Cry2Ab13 (Accession # ACG76120); Cry2Ab14 (Accession # ACG76121); Cry2Ab15 (Accession # HM037126); Cry2Ab16 (Accession # GQ866914); Cry2Ab17 (Accession # HQ439789); Cry2Ab18 (Accession # JN135255); Cry2Ab19 (Accession # JN135256); Cry2Ab20 (Accession # JN135257); Cry2Ab21 (Accession # JN135258); Cry2Ab22 (Accession # JN135259); Cry2Ab23 (Accession # JN135260); Cry2Ab24 (Accession # JN135261); Cry2Ab25 (Accession # JN415485); Cry2Ab26 (Accession # JN426946); Cry2Ab27 (Accession # JN415764); Cry2Ab28 (Accession # JN651494); Cry2Ac1 (Accession # CAA40536); Cry2Ac2 (Accession # AAG35410); Cry2Ac3 (Accession # AAQ52385); Cry2Ac4 (Accession # ABC95997); Cry2Ac5 (Accession # ABC74969); Cry2Ac6 (Accession # ABC74793); Cry2Ac7 (Accession # CAL18690); Cry2Ac8 (Accession # CAM09325); Cry2Ac9 (Accession # CAM09326); Cry2Ac10 (Accession # ABN15104); Cry2Ac11 (Accession # CAM83895); Cry2Ac12 (Accession # CAM83896); Cry2Ad1 (Accession # AAF09583); Cry2Ad2 (Accession # ABC86927); Cry2Ad3 (Accession # CAK29504); Cry2Ad4 (Accession # CAM32331); Cry2Ad5 (Accession # CA078739); Cry2Ae1 (Accession # AAQ52362); Cry2Af1 (Accession # AB030519); Cry2Af2 (Accession # GQ866915); Cry2Ag1 (Accession # ACH91610); Cry2Ah1 (Accession # EU939453); Cry2Ah2 (Accession # ACL80665); Cry2Ah3 (Accession # GU073380); Cry2Ah4 (Accession # KC156702); Cry2Ai1 (Accession # FJ788388); Cry2Aj (Accession #); Cry2Ak1 (Accession # KC156660); Cry2Ba1 (Accession # KC156658); Cry3Aa1 (Accession # AAA22336); Cry3Aa2 (Accession # AAA22541); Cry3Aa3 (Accession # CAA68482); Cry3Aa4 (Accession # AAA22542); Cry3Aa5 (Accession # AAA50255); Cry3Aa6 (Accession # AAC43266); Cry3Aa7 (Accession # CAB41411); Cry3Aa8 (Accession # AAS79487); Cry3Aa9 (Accession # AAW05659); Cry3Aa10 (Accession # AAU29411); Cry3Aa11 (Accession # AAW82872); Cry3Aa12 (Accession # ABY49136); Cry3Ba1 (Accession # CAA34983); Cry3Ba2 (Accession # CAA00645); Cry3Ba3 (Accession # JQ397327); Cry3Bb1 (Accession # AAA22334); Cry3Bb2 (Accession # AAA74198); Cry3Bb3 (Accession #115475); Cry3Ca1 (Accession # CAA42469); Cry4Aa1 (Accession # CAA68485); Cry4Aa2 (Accession # BAA00179); Cry4Aa3 (Accession # CAD30148); Cry4Aa4 (Accession # AFB18317); Cry4A-like (Accession # AAY96321); Cry4Ba1 (Accession # CAA30312); Cry4Ba2 (Accession # CAA30114); Cry4Ba3 (Accession # AAA22337); Cry4Ba4 (Accession # BAA00178); Cry4Ba5 (Accession # CAD30095); Cry4Ba-like (Accession # ABC47686); Cry4Ca1 (Accession # EU646202); Cry4Cb1 (Accession # FJ403208); Cry4Cb2 (Accession # FJ597622); Cry4Cc1 (Accession # FJ403207); Cry5Aa1 (Accession # AAA67694); Cry5Ab1 (Accession # AAA67693); Cry5Ac1 (Accession #134543); Cry5Ad1 (Accession # ABQ82087); Cry5Ba1 (Accession # AAA68598); Cry5Ba2 (Accession # ABW88931); Cry5Ba3 (Accession # AFJ04417); Cry5Ca1 (Accession # HM461869); Cry5Ca2 (Accession # ZP_04123426); Cry5Da1 (Accession # HM461870); Cry5Da2 (Accession # ZP_04123980); Cry5Ea1 (Accession # HM485580); Cry5Ea2 (Accession # ZP_04124038); Cry6Aa1 (Accession # AAA22357); Cry6Aa2 (Accession # AAM46849); Cry6Aa3 (Accession # ABH03377); Cry6Ba1 (Accession # AAA22358); Cry7Aa1 (Accession # AAA22351); Cry7Ab1 (Accession # AAA21120); Cry7Ab2 (Accession # AAA21121); Cry7Ab3 (Accession # ABX24522); Cry7Ab4 (Accession # EU380678); Cry7Ab5 (Accession # ABX79555); Cry7Ab6 (Accession # ACI44005); Cry7Ab7 (Accession # ADB89216); Cry7Ab8 (Accession # GU145299); Cry7Ab9 (Accession # ADD92572); Cry7Ba1 (Accession # ABB70817); Cry7Bb1 (Accession # KC156653); Cry7Ca1 (Accession # ABR67863); Cry7Cb1 (Accession # KC156698); Cry7Da1 (Accession # ACQ99547); Cry7Da2 (Accession # HM572236); Cry7Da3 (Accession # KC156679); Cry7Ea1 (Accession # HM035086); Cry7Ea2 (Accession # HM132124); Cry7Ea3 (Accession # EEM19403); Cry7Fa1 (Accession # HM035088); Cry7Fa2 (Accession # EEM19090); Cry7Fb1 (Accession # HM572235); Cry7Fb2 (Accession # KC156682); Cry7Ga1 (Accession # HM572237); Cry7Ga2 (Accession # KC156669); Cry7Gb1 (Accession # KC156650); Cry7Gc1 (Accession # KC156654); Cry7Gd1 (Accession # KC156697); Cry7Ha1 (Accession # KC156651); Cry7Ia1 (Accession # KC156665); Cry7Ja1 (Accession # KC156671); Cry7Ka1 (Accession # KC156680); Cry7Kb1 (Accession # BAM99306); Cry7La1 (Accession # BAM99307); Cry8Aa1 (Accession # AAA21117); Cry8Ab1 (Accession # EU044830); Cry8Ac1 (Accession # KC156662); Cry8Ada (Accession # KC156684); Cry8Ba1 (Accession # AAA21118); Cry8Bb1 (Accession # CAD57542); Cry8Bc1 (Accession # CAD57543); Cry8Ca1 (Accession # AAA21119); Cry8Ca2 (Accession # AAR98783); Cry8Ca3 (Accession # EU625349); Cry8Ca4 (Accession # ADB54826); Cry8Da1 (Accession # BAC07226); Cry8Da2 (Accession # BD133574); Cry8Da3 (Accession # BD133575); Cry8Db1 (Accession # BAF93483); Cry8Ea1 (Accession # AAQ73470); Cry8Ea2 (Accession # EU047597); Cry8Ea3 (Accession # KC855216); Cry8Fa1 (Accession # AAT48690); Cry8Fa2 (Accession # HQ174208); Cry8Fa3 (Accession # AFH78109); Cry8Ga1 (Accession # AAT46073); Cry8Ga2 (Accession # ABC42043); Cry8Ga3 (Accession # FJ198072); Cry8Ha1 (Accession # AAW81032); Cry8Ia1 (Accession # EU381044); Cry8Ia2 (Accession # GU073381); Cry8Ia3 (Accession # HM044664); Cry8Ia4 (Accession # KC156674); Cry8Ib1 (Accession # GU325772); Cry8Ib2 (Accession # KC156677); Cry8Ja1 (Accession # EU625348); Cry8Ka1 (Accession # FJ422558); Cry8Ka2 (Accession #
ACN87262); Cry8Kb1 (Accession # HM123758); Cry8Kb2 (Accession # KC156675); Cry8La1 (Accession # GU325771); Cry8Ma1 (Accession # HM044665); Cry8Ma2 (Accession # EEM86551); Cry8Ma3 (Accession # HM210574); Cry8Na1 (Accession # HM640939); Cry8Pa1 (Accession # HQ388415); Cry8Qa1 (Accession # HQ441166); Cry8Qa2 (Accession # KC152468); Cry8Ra1 (Accession # AFP87548); Cry8Sa1 (Accession # JQ740599); Cry8Ta1 (Accession # KC156673); Cry8-like (Accession # FJ770571); Cry8-like (Accession # ABS53003); Cry9Aa1 (Accession # CAA41122); Cry9Aa2 (Accession # CAA41425); Cry9Aa3 (Accession # GQ249293); Cry9Aa4 (Accession # GQ249294); Cry9Aa5 (Accession # JX174110); Cry9Aa like (Accession # AAQ52376); Cry9Ba1 (Accession # CAA52927); Cry9Ba2 (Accession # GU299522); Cry9Bb1 (Accession # AAV28716); Cry9Ca1 (Accession # CAA85764); Cry9Ca2 (Accession # AAQ52375); Cry9Da1 (Accession # BAA19948); Cry9Da2 (Accession # AAB97923); Cry9Da3 (Accession # GQ249293); Cry9Da4 (Accession # GQ249297); Cry9Db1 (Accession # AAX78439); Cry9Dc1 (Accession # KC156683); Cry9Ea1 (Accession # BAA34908); Cry9Ea2 (Accession # AA012908); Cry9Ea3 (Accession # ABM21765); Cry9Ea4 (Accession # ACE88267); Cry9Ea5 (Accession # ACF04743); Cry9Ea6 (Accession # ACG63872); Cry9Ea7 (Accession # FJ380927); Cry9Ea8 (Accession # GQ249292); Cry9Ea9 (Accession # JN651495); Cry9Eb1 (Accession # CAC50780); Cry9Eb2 (Accession # GQ249298); Cry9Eb3 (Accession # KC156646); Cry9Ec1 (Accession # AAC63366); Cry9Ed1 (Accession # AAX78440); Cry9Ee1 (Accession # GQ249296); Cry9Ee2 (Accession # KC156664); Cry9Fa1 (Accession # KC156692); Cry9Ga1 (Accession # KC156699); Cry9-like (Accession # AAC63366); Cry10Aa1 (Accession # AAA22614); Cry10Aa2 (Accession # E00614); Cry10Aa3 (Accession # CAD30098); Cry10Aa4 (Accession # AFB18318); Cry10A-like (Accession # DQ167578); Cry11Aa1 (Accession # AAA22352); Cry11Aa2 (Accession # AAA22611); Cry11Aa3 (Accession # CAD30081); Cry11Aa4 (Accession # AFB18319); Cry11Aa-like (Accession # DQ166531); Cry11Ba1 (Accession # CAA60504); Cry11Bb1 (Accession # AAC97162); Cry11Bb2 (Accession # HM068615); Cry12Aa1 (Accession # AAA22355); Cry13Aa1 (Accession # AAA22356); Cry14Aa1 (Accession # AAA21516); Cry14Ab1 (Accession # KC156652); Cry11Aa1 (Accession # AAA22333); Cry16Aa1 (Accession # CAA63860); Cry11Aa1 (Accession # CAA67841); Cry11Aa1 (Accession # CAA67506); Cry18Ba1 (Accession # AAF89667); Cry18Ca1 (Accession # AAF89668); Cry19Aa1 (Accession # CAA68875); Cry19Ba1 (Accession # BAA32397); Cry19Ca1 (Accession # AFM37572); Cry20Aa1 (Accession # AAB93476); Cry20Ba1 (Accession # ACS93601); Cry20Ba2 (Accession # KC156694); Cry20-like (Accession # GQ144333); Cry21Aa1 (Accession #132932); Cry21Aa2 (Accession #166477); Cry21Ba1 (Accession # BAC06484); Cry21Ca1 (Accession # JF521577); Cry21Ca2 (Accession # KC156687); Cry21Da1 (Accession # JF521578); Cry22Aa1 (Accession #134547); Cry22Aa2 (Accession # CAD43579); Cry22Aa3 (Accession # ACD93211); Cry22Ab1 (Accession # AAK50456); Cry22Ab2 (Accession # CAD43577); Cry22Ba1 (Accession # CAD43578); Cry22Bb1 (Accession # KC156672); Cry23Aa1 (Accession # AAF76375); Cry24Aa1 (Accession # AAC61891); Cry24Ba1 (Accession # BAD32657); Cry24Ca1 (Accession # CAJ43600); Cry25Aa1 (Accession # AAC61892); Cry26Aa1 (Accession # AAD25075); Cry27Aa1 (Accession # BAA82796); Cry28Aa1 (Accession # AAD24189); Cry28Aa2 (Accession # AAG00235); Cry29Aa1 (Accession # CAC80985); Cry30Aa1 (Accession # CAC80986); Cry30Ba1 (Accession # BAD00052); Cry30Ca1 (Accession # BAD67157); Cry30Ca2 (Accession # ACU24781); Cry30Da1 (Accession # EF095955); Cry30Db1 (Accession # BAE80088); Cry30Ea1 (Accession # ACC95445); Cry30Ea2 (Accession # FJ499389); Cry30Fa1 (Accession # ACI22625); Cry30Ga1 (Accession # ACG60020); Cry30Ga2 (Accession # HQ638217); Cry31Aa1 (Accession # BAB11757); Cry31Aa2 (Accession # AAL87458); Cry31Aa3 (Accession # BAE79808); Cry31Aa4 (Accession # BAF32571); Cry31Aa5 (Accession # BAF32572); Cry31Aa6 (Accession # BAI44026); Cry31Ab1 (Accession # BAE79809); Cry31Ab2 (Accession # BAF32570); Cry31Ac1 (Accession # BAF34368); Cry31Ac2 (Accession # AB731600); Cry31Ad1 (Accession # BAI44022); Cry32Aa1 (Accession # AAG36711); Cry32Aa2 (Accession # GU063849); Cry32Ab1 (Accession # GU063850); Cry32Ba1 (Accession # BAB78601); Cry32Ca1 (Accession # BAB78602); Cry32Cb1 (Accession # KC156708); Cry32Da1 (Accession # BAB78603); Cry32Ea1 (Accession # GU324274); Cry32Ea2 (Accession # KC156686); Cry32Eb1 (Accession # KC156663); Cry32Fa1 (Accession # KC156656); Cry32Ga1 (Accession # KC156657); Cry32Ha1 (Accession # KC156661); Cry32Hb1 (Accession # KC156666); Cry32Ia1 (Accession # KC156667); Cry32Ja1 (Accession # KC156685); Cry32Ka1 (Accession # KC156688); Cry32La1 (Accession # KC156689); Cry32Ma1 (Accession # KC156690); Cry32Mb1 (Accession # KC156704); Cry32Na1 (Accession # KC156691); Cry32Oa1 (Accession # KC156703); Cry32Pa1 (Accession # KC156705); Cry32Qa1 (Accession # KC156706); Cry32Ra1 (Accession # KC156707); Cry32Sa1 (Accession # KC156709); Cry32Ta1 (Accession # KC156710); Cry32Ua1 (Accession # KC156655); Cry33Aa1 (Accession # AAL26871); Cry34Aa1 (Accession # AAG50341); Cry34Aa2 (Accession # AAK64560); Cry34Aa3 (Accession # AAT29032); Cry34Aa4 (Accession # AAT29030); Cry34Ab1 (Accession # AAG41671); Cry34Ac1 (Accession # AAG50118); Cry34Ac2 (Accession # AAK64562); Cry34Ac3 (Accession # AAT29029); Cry34Ba1 (Accession # AAK64565); Cry34Ba2 (Accession # AAT29033); Cry34Ba3 (Accession # AAT29031); Cry35Aa1 (Accession # AAG50342); Cry35Aa2 (Accession # AAK64561); Cry35Aa3 (Accession # AAT29028); Cry35Aa4 (Accession # AAT29025); Cry35Ab1 (Accession # AAG41672); Cry35Ab2 (Accession # AAK64563); Cry35Ab3 (Accession # AY536891); Cry35Ac1 (Accession # AAG50117); Cry35Ba1 (Accession # AAK64566); Cry35Ba2 (Accession # AAT29027); Cry35Ba3 (Accession # AAT29026); Cry36Aa1 (Accession # AAK64558); Cry37Aa1 (Accession # AAF76376); Cry38Aa1 (Accession # AAK64559); Cry39Aa1 (Accession # BAB72016); Cry40Aa1 (Accession # BAB72018); Cry40Ba1 (Accession # BAC77648); Cry40Ca1 (Accession # EU381045); Cry40Da1 (Accession # ACF15199); Cry41Aa1 (Accession # BAD35157); Cry41Ab1 (Accession # BAD35163); Cry41Ba1 (Accession # HM461871); Cry41Ba2 (Accession # ZP_04099652); Cry42Aa1 (Accession # BAD35166); Cry43Aa1 (Accession # BAD15301); Cry43Aa2 (Accession # BAD95474); Cry43Ba1 (Accession # BAD15303); Cry43Ca1 (Accession # KC156676); Cry43Cb1 (Accession # KC156695); Cry43Cc1 (Accession # KC156696); Cry43-like (Accession # BAD15305); Cry44Aa (Accession # BAD08532); Cry45Aa (Accession # BAD22577); Cry46Aa (Accession # BAC79010); Cry46Aa2 (Accession # BAG68906); Cry46Ab (Accession # BAD35170); Cry47Aa (Accession # AAY24695); Cry48Aa (Accession # CAJ18351); Cry48Aa2 (Accession # CAJ86545); Cry48Aa3 (Accession # CAJ86546); Cry48Ab (Accession # CAJ86548); Cry48Ab2 (Accession # CAJ86549); Cry49Aa (Accession # CAH56541); Cry49Aa2 (Accession # CAJ86541); Cry49Aa3 (Accession # CAJ86543); Cry49Aa4 (Accession # CAJ86544); Cry49Ab1 (Accession # CAJ86542); Cry50Aa1 (Accession # BAE86999); Cry50Ba1 (Accession # GU446675); Cry50Ba2 (Accession # GU446676); Cry51Aa1 (Accession # ABI14444); Cry51Aa2 (Accession # GU570697); Cry52Aa1 (Accession # EF613489); Cry52Ba1 (Accession # FJ361760); Cry53Aa1 (Accession # EF633476); Cry53Ab1 (Accession # FJ361759); Cry54Aa1 (Accession # ACA52194); Cry54Aa2 (Accession # GQ140349); Cry54Ba1 (Accession # GU446677); Cry55Aa1 (Accession # ABW88932); Cry54Ab1 (Accession # JQ916908); Cry55Aa2 (Accession # AAE33526); Cry56Aa1 (Accession # ACU57499); Cry56Aa2 (Accession # GQ483512); Cry56Aa3 (Accession # JX025567); Cry57Aa1 (Accession # ANC87261); Cry58Aa1 (Accession # ANC87260); Cry59Ba1 (Accession # JN790647); Cry59Aa1 (Accession # ACR43758); Cry60Aa1 (Accession # ACU24782); Cry60Aa2 (Accession # EAO57254); Cry60Aa3 (Accession # EEM99278); Cry60Ba1 (Accession # GU810818); Cry60Ba2 (Accession # EAO57253); Cry60Ba3 (Accession # EEM99279); Cry61Aa1 (Accession # HM035087); Cry61Aa2 (Accession # HM132125); Cry61Aa3 (Accession # EEM19308); Cry62Aa1 (Accession # HM054509); Cry63Aa1 (Accession # BAI44028); Cry64Aa1 (Accession # BAJ05397); Cry65Aa1 (Accession # HM461868); Cry65Aa2 (Accession # ZP_04123838); Cry66Aa1 (Accession # HM485581); Cry66Aa2 (Accession # ZP_04099945); Cry67Aa1 (Accession # HM485582); Cry67Aa2 (Accession # ZP_04148882); Cry68Aa1 (Accession # HQ113114); Cry69Aa1 (Accession # HQ401006); Cry69Aa2 (Accession # JQ821388); Cry69Ab1 (Accession # JN209957); Cry70Aa1 (Accession # JN646781); Cry70Ba1 (Accession # ADO51070); Cry70Bb1 (Accession # EEL67276); Cry71Aa1 (Accession # JX025568); Cry72Aa1 (Accession # JX025569).
[0162] Examples of .delta.-endotoxins also include but are not limited to Cry1A proteins of U.S. Pat. Nos. 5,880,275 and 7,858,849; a DIG-3 or DIG-11 toxin (N-terminal deletion of .alpha.-helix 1 and/or .alpha.-helix 2 variants of Cry proteins such as Cry1A) of U.S. Pat. Nos. 8,304,604 and 8,304,605, Cry1B of U.S. patent application Ser. No. 10/525,318; Cry1C of U.S. Pat. No. 6,033,874; Cry1F of U.S. Pat. Nos. 5,188,960, 6,218,188; Cry1A/F chimeras of U.S. Pat. Nos. 7,070,982; 6,962,705 and 6,713,063); a Cry2 protein such as Cry2Ab protein of U.S. Pat. No. 7,064,249); a Cry3A protein including but not limited to an engineered hybrid insecticidal protein (eHIP) created by fusing unique combinations of variable regions and conserved blocks of at least two different Cry proteins (US Patent Application Publication Number 2010/0017914); a Cry4 protein; a Cry5 protein; a Cry6 protein; Cry8 proteins of U.S. Pat. Nos. 7,329,736, 7,449,552, 7,803,943, 7,476,781, 7,105,332, 7,378,499 and 7,462,760; a Cry9 protein such as such as members of the Cry9A, Cry9B, Cry9C, Cry9D, Cry9E, and Cry9F families; a Cry15 protein of Naimov, et al., (2008) Applied and Environmental Microbiology 74:7145-7151; a Cry22, a Cry34Ab1 protein of U.S. Pat. Nos. 6,127,180, 6,624,145 and 6,340,593; a CryET33 and CryET34 protein of U.S. Pat. Nos. 6,248,535, 6,326,351, 6,399,330, 6,949,626, 7,385,107 and 7,504,229; a CryET33 and CryET34 homologs of US Patent Publication Number 2006/0191034, 2012/0278954, and PCT Publication Number WO 2012/139004; a Cry35Ab1 protein of U.S. Pat. Nos. 6,083,499, 6,548,291 and 6,340,593; a Cry46 protein, a Cry 51 protein, a Cry binary toxin; a TIC901 or related toxin; TIC807 of US 2008/0295207; ET29, ET37, TIC809, TIC810, TIC812, TIC127, TIC128 of PCT US 2006/033867; TIC1100, TIC 860, a TIC867, a TIC868, TIC869, and TIC836 of US Patent Publication Number 2016/0108428. AXMI-027, AXMI-036, and AXMI-038 of U.S. Pat. No. 8,236,757; AXMI-031, AXMI-039, AXMI-040, AXMI-049 of U.S. Pat. No. 7,923,602; AXMI-018, AXMI-020, and AXMI-021 of WO 2006/083891; AXMI-010 of WO 2005/038032; AXMI-003 of WO 2005/021585; AXMI-008 of US 2004/0250311; AXMI-006 of US 2004/0216186; AXMI-007 of US 2004/0210965; AXMI-009 of US 2004/0210964; AXMI-014 of US 2004/0197917; AXMI-004 of US 2004/0197916; AXMI-028 and AXMI-029 of WO 2006/119457; AXMI-007, AXMI-008, AXMI-0080rf2, AXMI-009, AXMI-014 and AXMI-004 of WO 2004/074462; AXMI-150 of U.S. Pat. No. 8,084,416; AXMI-205 of US20110023184; AXMI-011, AXMI-012, AXMI-013, AXMI-015, AXMI-019, AXMI-044, AXMI-037, AXMI-043, AXMI-033, AXMI-034, AXMI-022, AXMI-023, AXMI-041, AXMI-063, and AXMI-064 of US 2011/0263488; AXMI-R1 and related proteins of US 2010/0197592; AXMI221Z, AXMI222z, AXMI223z, AXMI224z and AXMI225z of WO 2011/103248; AXMI218, AXMI219, AXMI220, AXMI226, AXMI227, AXMI228, AXMI229, AXMI230, and AXMI231 of WO11/103247; AXMI-115, AXMI-113, AXMI-005, AXMI-163 and AXMI-184 of U.S. Pat. No. 8,334,431; AXMI-001, AXMI-002, AXMI-030, AXMI-035, and AXMI-045 of US 2010/0298211; AXMI-066 and AXMI-076 of US20090144852; AXMI128, AXMI130, AXMI131, AXMI133, AXMI140, AXMI141, AXMI142, AXMI143, AXMI144, AXMI146, AXMI148, AXMI149, AXMI152, AXMI153, AXMI154, AXMI155, AXMI156, AXMI157, AXMI158, AXMI162, AXMI165, AXMI166, AXMI167, AXMI168, AXMI169, AXMI170, AXMI171, AXMI172, AXMI173, AXMI174, AXMI175, AXMI176, AXMI177, AXMI178, AXMI179, AXMI180, AXMI181, AXMI182, AXMI185, AXMI186, AXMI187, AXMI188, AXMI189 of U.S. Pat. No. 8,318,900; AXMI079, AXMI080, AXMI081, AXMI082, AXMI091, AXMI092, AXMI096, AXMI097, AXMI098, AXMI099, AXMI100, AXMI101, AXMI102, AXMI103, AXMI104, AXMI107, AXMI108, AXMI109, AXMI110, AXMI111, AXMI112, AXMI114, AXMI116, AXMI117, AXMI118, AXMI119, AXMI120, AXMI121, AXMI122, AXMI123, AXMI124, AXMI1257, AXMI1268, AXMI127, AXMI129, AXMI164, AXMI151, AXMI161, AXMI183, AXMI132, AXMI138, AXMI137 of US 2010/0005543; and Cry proteins such as Cry1A and Cry3A having modified proteolytic sites of U.S. Pat. No. 8,319,019; a Cry1Ac, Cry2Aa and Cry1Ca toxin protein from Bacillus thuringiensis strain VBTS 2528 of US Patent Application Publication Number 2011/0064710, and an IP1B of PCT publication number WO 2016/061197. Other Cry proteins are well known to one skilled in the art (see, Crickmore, et al., "Bacillus thuringiensis toxin nomenclature" (2011), at lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/which can be accessed on the world-wide web using the "www" prefix). The insecticidal activity of Cry proteins is well known to one skilled in the art (for review, see, van Frannkenhuyzen, (2009) J. Invert. Path. 101:1-16). The use of Cry proteins as transgenic plant traits is well known to one skilled in the art and Cry-transgenic plants including but not limited to Cry1Ac, Cry1Ac+Cry2Ab, Cry1Ab, Cry1A.105, Cry1F, Cry1Fa2, Cry1F+Cry1Ac, Cry2Ab, Cry3A, mCry3A, Cry3Bb1, Cry34Ab1, Cry35Ab1, Vip3A, mCry3A, Cry9c and CBI-Bt have received regulatory approval (see, Sanahuja, (2011) Plant Biotech Journal 9:283-300 and the CERA (2010) GM Crop Database Center for Environmental Risk Assessment (CERA), ILSI Research Foundation, Washington D.C. at cera-gmc.org/index.php?action=gm_crop_database which can be accessed on the world-wide web using the "www" prefix). More than one pesticidal proteins well known to one skilled in the art can also be expressed in plants such as Vip3Ab & Cry1Fa (US2012/0317682), Cry1BE & Cry1F (US2012/0311746), Cry1CA & Cry1AB (US2012/0311745), Cry1F & CryCa (US2012/0317681), Cry1DA & Cry1BE (US2012/0331590), Cry1DA & Cry1Fa (US2012/0331589), Cry1AB & Cry1BE (US2012/0324606), and Cry1Fa & Cry2Aa, Cry1I or Cry1E (US2012/0324605)); Cry34Ab/35Ab and Cry6Aa (US20130167269); Cry34Ab/VCry35Ab & Cry3Aa (US20130167268); Cry3A and Cry1Ab or Vip3Aa (US20130116170); and Cry1F, Cry34Ab1, and Cry35Ab1 (PCT/US2010/060818). Pesticidal proteins also include insecticidal lipases including lipid acyl hydrolases of U.S. Pat. No. 7,491,869, and cholesterol oxidases such as from Streptomyces (Purcell et al. (1993) Biochem Biophys Res Commun 15:1406-1413). Pesticidal proteins also include VIP (vegetative insecticidal proteins) toxins of U.S. Pat. Nos. 5,877,012, 6,107,279, 6,137,033, 7,244,820, 7,615,686, and 8,237,020, and the like. Other VIP proteins are well known to one skilled in the art (see, lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/vip.html which can be accessed on the world-wide web using the "www" prefix). Pesticidal proteins also include toxin complex (TC) proteins, obtainable from organisms such as Xenorhabdus, Photorhabdus and Paenibacillus (see, U.S. Pat. Nos. 7,491,698 and 8,084,418). Some TC proteins have "stand alone" insecticidal activity and other TC proteins enhance the activity of the stand-alone toxins produced by the same given organism. The toxicity of a "stand-alone" TC protein (from Photorhabdus, Xenorhabdus or Paenibacillus, for example) can be enhanced by one or more TC protein "potentiators" derived from a source organism of a different genus. There are three main types of TC proteins. As referred to herein, Class A proteins ("Protein A") are stand-alone toxins. Class B proteins ("Protein B") and Class C proteins ("Protein C") enhance the toxicity of Class A proteins. Examples of Class A proteins are TcbA, TcdA, XptA1 and XptA2. Examples of Class B proteins are TcaC, TcdB, XptB1Xb and XptC1Wi. Examples of Class C proteins are TccC, XptC1Xb and XptB1Wi. Pesticidal proteins also include spider, snake and scorpion venom proteins. Examples of spider venom peptides include but are not limited to lycotoxin-1 peptides and mutants thereof (U.S. Pat. No. 8,334,366).
[0163] (C) A polynucleotide encoding an insect-specific hormone or pheromone such as an ecdysteroid and juvenile hormone, a variant thereof, a mimetic based thereon or an antagonist or agonist thereof. See, for example, the disclosure by Hammock, et al., (1990) Nature 344:458, of baculovirus expression of cloned juvenile hormone esterase, an inactivator of juvenile hormone.
[0164] (D) A polynucleotide encoding an insect-specific peptide which, upon expression, disrupts the physiology of the affected pest. For example, see the disclosures of, Regan, (1994) J. Biol. Chem. 269:9 (expression cloning yields DNA coding for insect diuretic hormone receptor); Pratt, et al., (1989) Biochem. Biophys. Res. Comm 163:1243 (an allostatin is identified in Diploptera puntata); Chattopadhyay, et al., (2004) Critical Reviews in Microbiology 30(1):33-54; Zjawiony, (2004) J Nat Prod 67(2):300-310; Carlini and Grossi-de-Sa, (2002) Toxicon 40(11):1515-1539; Ussuf, et al., (2001) Curr Sci. 80(7):847-853 and Vasconcelos and Oliveira, (2004) Toxicon 44(4):385-403. See also, U.S. Pat. No. 5,266,317 to Tomalski, et al., who disclose genes encoding insect-specific toxins.
[0165] (E) A polynucleotide encoding an enzyme responsible for a hyperaccumulation of a monoterpene, a sesquiterpene, a steroid, hydroxamic acid, a phenylpropanoid derivative or another non-protein molecule with insecticidal activity.
[0166] (F) A polynucleotide encoding an enzyme involved in the modification, including the post-translational modification, of a biologically active molecule; for example, a glycolytic enzyme, a proteolytic enzyme, a lipolytic enzyme, a nuclease, a cyclase, a transaminase, an esterase, a hydrolase, a phosphatase, a kinase, a phosphorylase, a polymerase, an elastase, a chitinase and a glucanase, whether natural or synthetic. See, PCT Application WO 1993/02197 in the name of Scott, et al., which discloses the nucleotide sequence of a callase gene. DNA molecules which contain chitinase-encoding sequences can be obtained, for example, from the ATCC under Accession Numbers 39637 and 67152. See also, Kramer, et al., (1993) Insect Biochem. Molec. Biol. 23:691, who teach the nucleotide sequence of a cDNA encoding tobacco hookworm chitinase and Kawalleck, et al., (1993) Plant Molec. Biol. 21:673, who provide the nucleotide sequence of the parsley ubi4-2 polyubiquitin gene, and U.S. Pat. Nos. 6,563,020; 7,145,060 and 7,087,810.
[0167] (G) A polynucleotide encoding a molecule that stimulates signal transduction. For example, see the disclosure by Botella, et al., (1994) Plant Molec. Biol. 24:757, of nucleotide sequences for mung bean calmodulin cDNA clones, and Griess, et al., (1994) Plant Physiol. 104:1467, who provide the nucleotide sequence of a maize calmodulin cDNA clone.
[0168] (H) A polynucleotide encoding a hydrophobic moment peptide. See, PCT Application WO 1995/16776 and U.S. Pat. No. 5,580,852 disclosure of peptide derivatives of Tachyplesin which inhibit fungal plant pathogens) and PCT Application WO 1995/18855 and U.S. Pat. No. 5,607,914 (teaches synthetic antimicrobial peptides that confer disease resistance).
[0169] (I) A polynucleotide encoding a membrane permease, a channel former or a channel blocker. For example, see the disclosure by Jaynes, et al., (1993) Plant Sci. 89:43, of heterologous expression of a cecropin-beta lytic peptide analog to render transgenic tobacco plants resistant to Pseudomonas solanacearum.
[0170] (J) A gene encoding a viral-invasive protein or a complex toxin derived therefrom. For example, the accumulation of viral coat proteins in transformed plant cells imparts resistance to viral infection and/or disease development effected by the virus from which the coat protein gene is derived, as well as by related viruses. See, Beachy, et al., (1990) Ann. Rev. Phytopathol. 28:451. Coat protein-mediated resistance has been conferred upon transformed plants against alfalfa mosaic virus, cucumber mosaic virus, tobacco streak virus, potato virus X, potato virus Y, tobacco etch virus, tobacco rattle virus and tobacco mosaic virus. Id.
[0171] (K) A gene encoding an insect-specific antibody or an immunotoxin derived therefrom. Thus, an antibody targeted to a critical metabolic function in the insect gut would inactivate an affected enzyme, killing the insect. Cf. Taylor, et al., Abstract #497, SEVENTH INT'L SYMPOSIUM ON MOLECULAR PLANT-MICROBE INTERACTIONS (Edinburgh, Scotland, 1994) (enzymatic inactivation in transgenic tobacco via production of single-chain antibody fragments).
[0172] (L) A gene encoding a virus-specific antibody. See, for example, Tavladoraki, et al., (1993) Nature 366:469, who show that transgenic plants expressing recombinant antibody genes are protected from virus attack.
[0173] (M) A polynucleotide encoding a developmental-arrestive protein produced in nature by a pathogen or a parasite. Thus, fungal endo alpha-1,4-D-polygalacturonases facilitate fungal colonization and plant nutrient release by solubilizing plant cell wall homo-alpha-1,4-D-galacturonase. See, Lamb, et al., (1992) Bio/Technology 10:1436. The cloning and characterization of a gene which encodes a bean endopolygalacturonase-inhibiting protein is described by Toubart, et al., (1992) Plant J. 2:367.
[0174] (N) A polynucleotide encoding a developmental-arrestive protein produced in nature by a plant. For example, Logemann, et al., (1992) Bio/Technology 10:305, have shown that transgenic plants expressing the barley ribosome-inactivating gene have an increased resistance to fungal disease.
[0175] (O) Genes involved in the Systemic Acquired Resistance (SAR) Response and/or the pathogenesis related genes. Briggs, (1995) Current Biology 5(2), Pieterse and Van Loon, (2004) Curr. Opin. Plant Bio. 7(4):456-64 and Somssich, (2003) Cell 113(7):815-6.
[0176] (P) Antifungal genes (Cornelissen and Melchers, (1993) Pl. Physiol. 101:709-712 and Parijs, et al., (1991) Planta 183:258-264 and Bushnell, et al., (1998) Can. J. of Plant Path. 20(2):137-149. Also see, U.S. patent application Ser. Nos. 09/950,933; 11/619,645; 11/657,710; 11/748,994; 11/774,121 and U.S. Pat. Nos. 6,891,085 and 7,306,946. LysM Receptor-like kinases for the perception of chitin fragments as a first step in plant defense response against fungal pathogens (US 2012/0110696).
[0177] (Q) Detoxification genes, such as for fumonisin, beauvericin, moniliformin and zearalenone and their structurally related derivatives. For example, see, U.S. Pat. Nos. 5,716,820; 5,792,931; 5,798,255; 5,846,812; 6,083,736; 6,538,177; 6,388,171 and 6,812,380.
[0178] (R) A polynucleotide encoding a Cystatin and cysteine proteinase inhibitors. See, U.S. Pat. No. 7,205,453.
[0179] (S) Defensin genes. See, WO 2003/000863 and U.S. Pat. Nos. 6,911,577; 6,855,865; 6,777,592 and 7,238,781.
[0180] (T) Genes conferring resistance to nematodes. See, e.g., PCT Application WO 1996/30517; PCT Application WO 1993/19181, WO 2003/033651 and Urwin, et al., (1998) Planta 204:472-479, Williamson, (1999) Curr Opin Plant Bio. 2(4):327-31; U.S. Pat. Nos. 6,284,948 and 7,301,069 and miR164 genes (WO 2012/058266).
[0181] (U) Genes that confer resistance to Phytophthora Root Rot, such as the Rps 1, Rps 1-a, Rps 1-b, Rps 1-c, Rps 1-d, Rps 1-e, Rps 1-k, Rps 2, Rps 3-a, Rps 3-b, Rps 3-c, Rps 4, Rps 5, Rps 6, Rps 7 and other Rps genes. See, for example, Shoemaker, et al., Phytophthora Root Rot Resistance Gene Mapping in Soybean, Plant Genome IV Conference, San Diego, Calif. (1995).
[0182] (V) Genes that confer resistance to Brown Stem Rot, such as described in U.S. Pat. No. 5,689,035 and incorporated by reference for this purpose.
[0183] (W) Genes that confer resistance to Colletotrichum, such as described in US Patent Application Publication US 2009/0035765 and incorporated by reference for this purpose. This includes the Rcg locus that may be utilized as a single locus conversion.
[0184] (X) Some embodiments relate to down-regulation of expression of target genes in insect pest species by interfering ribonucleic acid (RNA) molecules. PCT Publication WO 2007/074405 describes methods of inhibiting expression of target genes in invertebrate pests including Colorado potato beetle. PCT Publication WO 2005/110068 describes methods of inhibiting expression of target genes in invertebrate pests including in particular Western corn rootworm as a means to control insect infestation. Furthermore, PCT Publication WO 2009/091864 describes compositions and methods for the suppression of target genes from insect pest species including pests from the Lygus genus.
[0185] Nucleic acid molecules including silencing elements for targeting the vacuolar ATPase H subunit, useful for controlling a coleopteran pest population and infestation as described in US Patent Application Publication 2012/0198586. PCT Publication WO 2012/055982 describes ribonucleic acid (RNA or double stranded RNA) that inhibits or down regulates the expression of a target gene that encodes: an insect ribosomal protein such as the ribosomal protein L19, the ribosomal protein L40 or the ribosomal protein S27A; an insect proteasome subunit such as the Rpn6 protein, the Pros 25, the Rpn2 protein, the proteasome beta 1 subunit protein or the Pros beta 2 protein; an insect .beta.-coatomer of the COPI vesicle, the .gamma.-coatomer of the COPI vesicle, the .beta.'-coatomer protein or the .zeta.-coatomer of the COPI vesicle; an insect Tetraspanine 2 A protein which is a putative transmembrane domain protein; an insect protein belonging to the actin family such as Actin 5C; an insect ubiquitin-5E protein; an insect Sec23 protein which is a GTPase activator involved in intracellular protein transport; an insect crinkled protein which is an unconventional myosin which is involved in motor activity; an insect crooked neck protein which is involved in the regulation of nuclear alternative mRNA splicing; an insect vacuolar H+-ATPase G-subunit protein and an insect Tbp-1 such as Tat-binding protein. PCT publication WO 2007/035650 describes ribonucleic acid (RNA or double stranded RNA) that inhibits or down regulates the expression of a target gene that encodes Snf7. US Patent Application publication 2011/0054007 describes polynucleotide silencing elements targeting RPS10. US Patent Application publication 2014/0275208 and US2015/0257389 describe polynucleotide silencing elements targeting RyanR and PAT3. PCT publications WO 2016/060911, WO 2016/060912, WO 2016/060913, and WO 2016/060914 describe polynucleotide silencing elements targeting COPI coatomer subunit nucleic acid molecules that confer resistance to Coleopteran and Hemipteran pests. US Patent Application Publications 2012/029750, US 20120297501, and 2012/0322660 describe interfering ribonucleic acids (RNA or double stranded RNA) that functions upon uptake by an insect pest species to down-regulate expression of a target gene in said insect pest, wherein the RNA comprises at least one silencing element wherein the silencing element is a region of double-stranded RNA comprising annealed complementary strands, one strand of which comprises or consists of a sequence of nucleotides which is at least partially complementary to a target nucleotide sequence within the target gene. US Patent Application Publication 2012/0164205 describe potential targets for interfering double stranded ribonucleic acids for inhibiting invertebrate pests including: a Chd3 Homologous Sequence, a Beta-Tubulin Homologous Sequence, a 40 kDa V-ATPase Homologous Sequence, a EF1.alpha. Homologous Sequence, a 26S Proteosome Subunit p28 Homologous Sequence, a Juvenile Hormone Epoxide Hydrolase Homologous Sequence, a Swelling Dependent Chloride Channel Protein Homologous Sequence, a Glucose-6-Phosphate 1-Dehydrogenase Protein Homologous Sequence, an Act42A Protein Homologous Sequence, a ADP-Ribosylation Factor 1 Homologous Sequence, a Transcription Factor IIB Protein Homologous Sequence, a Chitinase Homologous Sequences, a Ubiquitin Conjugating Enzyme Homologous Sequence, a Glyceraldehyde-3-Phosphate Dehydrogenase Homologous Sequence, an Ubiquitin B Homologous Sequence, a Juvenile Hormone Esterase Homolog, and an Alpha Tubuliln Homologous Sequence.
[0186] ii. Transgenes that Confer Resistance to a Herbicide.
[0187] (A) A polynucleotide encoding resistance to a herbicide that inhibits the growing point or meristem, such as an imidazolinone or a sulfonylurea. Exemplary genes in this category code for mutant ALS and AHAS enzyme as described, for example, by Lee, et al., (1988) EMBO J. 7:1241 and Mild, et al., (1990) Theor. Appl. Genet. 80:449, respectively. See also, U.S. Pat. Nos. 5,605,011; 5,013,659; 5,141,870; 5,767,361; 5,731,180; 5,304,732; 4,761,373; 5,331,107; 5,928,937 and 5,378,824; U.S. patent application Ser. No. 11/683,737 and International Publication WO 1996/33270.
[0188] (B) A polynucleotide encoding a protein for resistance to Glyphosate (resistance imparted by mutant 5-enolpyruvl-3-phosphikimate synthase (EPSP) and aroA genes, respectively) and other phosphono compounds such as glufosinate (phosphinothricin acetyl transferase (PAT) and Streptomyces hygroscopicus phosphinothricin acetyl transferase (bar) genes), and pyridinoxy or phenoxy proprionic acids and cyclohexones (ACCase inhibitor-encoding genes). See, for example, U.S. Pat. No. 4,940,835 to Shah, et al., which discloses the nucleotide sequence of a form of EPSPS which can confer glyphosate resistance. U.S. Pat. No. 5,627,061 to Barry, et al., also describes genes encoding EPSPS enzymes. See also, U.S. Pat. Nos. 6,566,587; 6,338,961; 6,248,876 B1; 6,040,497; 5,804,425; 5,633,435; 5,145,783; 4,971,908; 5,312,910; 5,188,642; 5,094,945, 4,940,835; 5,866,775; 6,225,114 B1; 6,130,366; 5,310,667; 4,535,060; 4,769,061; 5,633,448; 5,510,471; Re. 36,449; RE 37,287 E and 5,491,288 and International Publications EP 1173580; WO 2001/66704; EP 1173581 and EP 1173582.
[0189] Glyphosate resistance is also imparted to plants that express a gene encoding a glyphosate oxido-reductase enzyme as described more fully in U.S. Pat. Nos. 5,776,760 and 5,463,175. In addition glyphosate resistance can be imparted to plants by the over expression of genes encoding glyphosate N-acetyltransferase. See, for example, U.S. Pat. Nos. 7,462,481; 7,405,074 and US Patent Application Publication Number US 2008/0234130. A DNA molecule encoding a mutant aroA gene can be obtained under ATCC Accession Number 39256, and the nucleotide sequence of the mutant gene is disclosed in U.S. Pat. No. 4,769,061 to Comai. EP Application Number 0 333 033 to Kumada, et al., and U.S. Pat. No. 4,975,374 to Goodman, et al., disclose nucleotide sequences of glutamine synthetase genes which confer resistance to herbicides such as L-phosphinothricin. The nucleotide sequence of a phosphinothricin-acetyl-transferase gene is provided in EP Application Numbers 0 242 246 and 0 242 236 to Leemans, et al.; De Greef, et al., (1989) Bio/Technology 7:61, describe the production of transgenic plants that express chimeric bar genes coding for phosphinothricin acetyl transferase activity. See also, U.S. Pat. Nos. 5,969,213; 5,489,520; 5,550,318; 5,874,265; 5,919,675; 5,561,236; 5,648,477; 5,646,024; 6,177,616 B1 and 5,879,903. Exemplary genes conferring resistance to phenoxy proprionic acids and cyclohexones, such as sethoxydim and haloxyfop, are the Acc1-S1, Acc1-S2 and Acc1-S3 genes described by Marshall, et al., (1992) Theor. Appl. Genet. 83:435.
[0190] (C) A polynucleotide encoding a protein for resistance to herbicide that inhibits photosynthesis, such as a triazine (psbA and gs+ genes) and a benzonitrile (nitrilase gene). Przibilla, et al., (1991) Plant Cell 3:169, describe the transformation of Chlamydomonas with plasmids encoding mutant psbA genes. Nucleotide sequences for nitrilase genes are disclosed in U.S. Pat. No. 4,810,648 to Stalker and DNA molecules containing these genes are available under ATCC Accession Numbers 53435, 67441 and 67442. Cloning and expression of DNA coding for a glutathione S-transferase is described by Hayes, et al., (1992) Biochem. J. 285:173.
[0191] (D) A polynucleotide encoding a protein for resistance to Acetohydroxy acid synthase, which has been found to make plants that express this enzyme resistant to multiple types of herbicides, has been introduced into a variety of plants (see, e.g., Hattori, et al., (1995) Mol Gen Genet. 246:419). Other genes that confer resistance to herbicides include: a gene encoding a chimeric protein of rat cytochrome P4507A1 and yeast NADPH-cytochrome P450 oxidoreductase (Shiota, et al., (1994) Plant Physiol 106:17), genes for glutathione reductase and superoxide dismutase (Aono, et al., (1995) Plant Cell Physiol 36:1687) and genes for various phosphotransferases (Datta, et al., (1992) Plant Mol Biol 20:619).
[0192] (E) A polynucleotide encoding resistance to a herbicide targeting Protoporphyrinogen oxidase (protox) which is necessary for the production of chlorophyll. The protox enzyme serves as the target for a variety of herbicidal compounds. These herbicides also inhibit growth of all the different species of plants present, causing their total destruction. The development of plants containing altered protox activity which are resistant to these herbicides are described in U.S. Pat. Nos. 6,288,306 B1; 6,282,837 B1 and 5,767,373 and International Publication WO 2001/12825.
[0193] (F) The aad-1 gene (originally from Sphingobium herbicidovorans) encodes the aryloxyalkanoate dioxygenase (AAD-1) protein. The trait confers tolerance to 2,4-dichlorophenoxyacetic acid and aryloxyphenoxypropionate (commonly referred to as "fop" herbicides such as quizalofop) herbicides. The aad-1 gene, itself, for herbicide tolerance in plants was first disclosed in WO 2005/107437 (see also, US 2009/0093366). The aad-12 gene, derived from Delftia acidovorans, which encodes the aryloxyalkanoate dioxygenase (AAD-12) protein that confers tolerance to 2,4-dichlorophenoxyacetic acid and pyridyloxyacetate herbicides by deactivating several herbicides with an aryloxyalkanoate moiety, including phenoxy auxin (e.g., 2,4-D, MCPA), as well as pyridyloxy auxins (e.g., fluoroxypyr, triclopyr).
[0194] (G) A polynucleotide encoding a herbicide resistant dicamba monooxygenase disclosed in US Patent Application Publication 2003/0135879 for imparting dicamba tolerance.
[0195] (H) A polynucleotide molecule encoding bromoxynil nitrilase (Bxn) disclosed in U.S. Pat. No. 4,810,648 for imparting bromoxynil tolerance.
[0196] (I) A polynucleotide molecule encoding phytoene (crtl) described in Misawa, et al., (1993) Plant J. 4:833-840 and in Misawa, et al., (1994) Plant J. 6:481-489 for norflurazon tolerance.
[0197] iii. Transgenes that Confer or Contribute to an Altered Grain Characteristic
[0198] (A) Altered fatty acids, for example, by (1) Down-regulation of stearoyl-ACP to increase stearic acid content of the plant. See, Knultzon, et al., (1992) Proc. Natl. Acad. Sci. USA 89:2624 and WO 1999/64579 (Genes to Alter Lipid Profiles in Corn); (2) Elevating oleic acid via FAD-2 gene modification and/or decreasing linolenic acid via FAD-3 gene modification (see, U.S. Pat. Nos. 6,063,947; 6,323,392; 6,372,965 and WO 1993/11245); (3) Altering conjugated linolenic or linoleic acid content, such as in WO 2001/12800; (4) Altering LEC1, AGP, Dekl, Superall, mil ps, various Ipa genes such as Ipa1, Ipa3, hpt or hggt. For example, see, WO 2002/42424, WO 1998/22604, WO 2003/011015, WO 2002/057439, WO 2003/011015, U.S. Pat. Nos. 6,423,886, 6,197,561, 6,825,397 and US Patent Application Publication Numbers US 2003/0079247, US 2003/0204870 and Rivera-Madrid, et al., (1995) Proc. Natl. Acad. Sci. 92:5620-5624; (5) Genes encoding delta-8 desaturase for making long-chain polyunsaturated fatty acids (U.S. Pat. Nos. 8,058,571 and 8,338,152), delta-9 desaturase for lowering saturated fats (U.S. Pat. No. 8,063,269), Primula delta 6-desaturase for improving omega-3 fatty acid profiles; (6) Isolated nucleic acids and proteins associated with lipid and sugar metabolism regulation, in particular, lipid metabolism protein (LMP) used in methods of producing transgenic plants and modulating levels of seed storage compounds including lipids, fatty acids, starches or seed storage proteins and use in methods of modulating the seed size, seed number, seed weights, root length and leaf size of plants (EP 2404499); (7) Altering expression of a High-Level Expression of Sugar-Inducible 2 (HSI2) protein in the plant to increase or decrease expression of HSI2 in the plant. Increasing expression of HSI2 increases oil content while decreasing expression of HSI2 decreases abscisic acid sensitivity and/or increases drought resistance (US Patent Application Publication Number 2012/0066794); (8) Expression of cytochrome b5 (Cb5) alone or with FAD2 to modulate oil content in plant seed, particularly to increase the levels of omega-3 fatty acids and improve the ratio of omega-6 to omega-3 fatty acids (US Patent Application Publication Number 2011/0191904); and (9) Nucleic acid molecules encoding wrinkled1-like polypeptides for modulating sugar metabolism (U.S. Pat. No. 8,217,223).
[0199] (B) Altered phosphorus content, for example, by the (1) introduction of a phytase-encoding gene would enhance breakdown of phytate, adding more free phosphate to the transformed plant. For example, see, Van Hartingsveldt, et al., (1993) Gene 127:87, for a disclosure of the nucleotide sequence of an Aspergillus niger phytase gene; and (2) modulating a gene that reduces phytate content. In maize, this, for example, could be accomplished, by cloning and then re-introducing DNA associated with one or more of the alleles, such as the LPA alleles, identified in maize mutants characterized by low levels of phytic acid, such as in WO 2005/113778 and/or by altering inositol kinase activity as in WO 2002/059324, US Patent Application Publication Number 2003/0009011, WO 2003/027243, US Patent Application Publication Number 2003/0079247, WO 1999/05298, U.S. Pat. No. 6,197,561, U.S. Pat. No. 6,291,224, U.S. Pat. No. 6,391,348, WO 2002/059324, US Patent Application Publication Number 2003/0079247, WO 1998/45448, WO 1999/55882, WO 2001/04147.
[0200] (C) Altered carbohydrates affected, for example, by altering a gene for an enzyme that affects the branching pattern of starch or, a gene altering thioredoxin such as NTR and/or TRX (see, U.S. Pat. No. 6,531,648. which is incorporated by reference for this purpose) and/or a gamma zein knock out or mutant such as cs27 or TUSC27 or en27 (see, U.S. Pat. No. 6,858,778 and US Patent Application Publication Number 2005/0160488, US Patent Application Publication Number 2005/0204418, which are incorporated by reference for this purpose). See, Shiroza, et al., (1988) J. Bacteriol. 170:810 (nucleotide sequence of Streptococcus mutant fructosyltransferase gene), Steinmetz, et al., (1985) Mol. Gen. Genet. 200:220 (nucleotide sequence of Bacillus subtilis levansucrase gene), Pen, et al., (1992) Bio/Technology 10:292 (production of transgenic plants that express Bacillus licheniformis alpha-amylase), Elliot, et al., (1993) Plant Molec. Biol. 21:515 (nucleotide sequences of tomato invertase genes), Sogaard, et al., (1993) J. Biol. Chem. 268:22480 (site-directed mutagenesis of barley alpha-amylase gene) and Fisher, et al., (1993) Plant Physiol. 102:1045 (maize endosperm starch branching enzyme II), WO 1999/10498 (improved digestibility and/or starch extraction through modification of UDP-D-xylose 4-epimerase, Fragile 1 and 2, Ref1, HCHL, C4H), U.S. Pat. No. 6,232,529 (method of producing high oil seed by modification of starch levels (AGP)). The fatty acid modification genes mentioned herein may also be used to affect starch content and/or composition through the interrelationship of the starch and oil pathways.
[0201] (D) Altered antioxidant content or composition, such as alteration of tocopherol or tocotrienols. For example, see, U.S. Pat. No. 6,787,683, US Patent Application Publication Number 2004/0034886 and WO 2000/68393 involving the manipulation of antioxidant levels and WO 2003/082899 through alteration of a homogentisate geranyl geranyl transferase (hggt).
[0202] (E) Altered essential seed amino acids. For example, see, U.S. Pat. No. 6,127,600 (method of increasing accumulation of essential amino acids in seeds), U.S. Pat. No. 6,080,913 (binary methods of increasing accumulation of essential amino acids in seeds), U.S. Pat. No. 5,990,389 (high lysine), WO 1999/40209 (alteration of amino acid compositions in seeds), WO 1999/29882 (methods for altering amino acid content of proteins), U.S. Pat. No. 5,850,016 (alteration of amino acid compositions in seeds), WO 1998/20133 (proteins with enhanced levels of essential amino acids), U.S. Pat. No. 5,885,802 (high methionine), U.S. Pat. No. 5,885,801 (high threonine), U.S. Pat. No. 6,664,445 (plant amino acid biosynthetic enzymes), U.S. Pat. No. 6,459,019 (increased lysine and threonine), U.S. Pat. No. 6,441,274 (plant tryptophan synthase beta subunit), U.S. Pat. No. 6,346,403 (methionine metabolic enzymes), U.S. Pat. No. 5,939,599 (high sulfur), U.S. Pat. No. 5,912,414 (increased methionine), WO 1998/56935 (plant amino acid biosynthetic enzymes), WO 1998/45458 (engineered seed protein having higher percentage of essential amino acids), WO 1998/42831 (increased lysine), U.S. Pat. No. 5,633,436 (increasing sulfur amino acid content), U.S. Pat. No. 5,559,223 (synthetic storage proteins with defined structure containing programmable levels of essential amino acids for improvement of the nutritional value of plants), WO 1996/01905 (increased threonine), WO 1995/15392 (increased lysine), US Patent Application Publication Number 2003/0163838, US Patent Application Publication Number 2003/0150014, US Patent Application Publication Number 2004/0068767, U.S. Pat. No. 6,803,498, WO 2001/79516.
[0203] iv. Genes that Control Male-Sterility
[0204] There are several methods of conferring genetic male sterility available, such as multiple mutant genes at separate locations within the genome that confer male sterility, as disclosed in U.S. Pat. Nos. 4,654,465 and 4,727,219 to Brar, et al., and chromosomal translocations as described by Patterson in U.S. Pat. Nos. 3,861,709 and 3,710,511. In addition to these methods, Albertsen, et al., U.S. Pat. No. 5,432,068, describe a system of nuclear male sterility which includes: identifying a gene which is critical to male fertility; silencing this native gene which is critical to male fertility; removing the native promoter from the essential male fertility gene and replacing it with an inducible promoter; inserting this genetically engineered gene back into the plant; and thus creating a plant that is male sterile because the inducible promoter is not "on" resulting in the male fertility gene not being transcribed. Fertility is restored by inducing or turning "on", the promoter, which in turn allows the gene that confers male fertility to be transcribed. Non-limiting examples include: (A) Introduction of a deacetylase gene under the control of a tapetum-specific promoter and with the application of the chemical N--Ac-PPT (WO 2001/29237); (B) Introduction of various stamen-specific promoters (WO 1992/13956, WO 1992/13957); and (C) Introduction of the barnase and the barstar gene (Paul, et al., (1992) Plant Mol. Biol. 19:611-622). For additional examples of nuclear male and female sterility systems and genes, see also, U.S. Pat. Nos. 5,859,341; 6,297,426; 5,478,369; 5,824,524; 5,850,014 and 6,265,640.
[0205] v. Genes that Create a Site for Site Specific DNA Integration.
[0206] This includes the introduction of FRT sites that may be used in the FLP/FRT system and/or Lox sites that may be used in the Cre/Loxp system. For example, see, Lyznik, et al., (2003) Plant Cell Rep 21:925-932 and WO 1999/25821. Other systems that may be used include the Gln recombinase of phage Mu (Maeser, et al., (1991) Vicki Chandler, The Maize Handbook ch. 118 (Springer-Verlag 1994), the Pin recombinase of E. coli (Enomoto, et al., 1983) and the R/RS system of the pSRi plasmid (Araki, et al., 1992).
[0207] vi. Genes that Affect Abiotic Stress Resistance
[0208] Including but not limited to flowering, ear and seed development, enhancement of nitrogen utilization efficiency, altered nitrogen responsiveness, drought resistance or tolerance, cold resistance or tolerance and salt resistance or tolerance and increased yield under stress. Non-limiting examples include: (A) For example, see: WO 2000/73475 where water use efficiency is altered through alteration of malate; U.S. Pat. Nos. 5,892,009, 5,965,705, 5,929,305, 5,891,859, 6,417,428, 6,664,446, 6,706,866, 6,717,034, 6,801,104, WO 2000/060089, WO 2001/026459, WO 2001/035725, WO 2001/034726, WO 2001/035727, WO 2001/036444, WO 2001/036597, WO 2001/036598, WO 2002/015675, WO 2002/017430, WO 2002/077185, WO 2002/079403, WO 2003/013227, WO 2003/013228, WO 2003/014327, WO 2004/031349, WO 2004/076638, WO 199809521; (B) WO 199938977 describing genes, including CBF genes and transcription factors effective in mitigating the negative effects of freezing, high salinity and drought on plants, as well as conferring other positive effects on plant phenotype; (C) US Patent Application Publication Number 2004/0148654 and WO 2001/36596 where abscisic acid is altered in plants resulting in improved plant phenotype such as increased yield and/or increased tolerance to abiotic stress; (D) WO 2000/006341, WO 2004/090143, U.S. Pat. Nos. 7,531,723 and 6,992,237 where cytokinin expression is modified resulting in plants with increased stress tolerance, such as drought tolerance, and/or increased yield. Also see, WO 2002/02776, WO 2003/052063, JP 2002/281975, U.S. Pat. No. 6,084,153, WO 2001/64898, U.S. Pat. No. 6,177,275 and U.S. Pat. No. 6,107,547 (enhancement of nitrogen utilization and altered nitrogen responsiveness); (E) For ethylene alteration, see, US Patent Application Publication Number 2004/0128719, US Patent Application Publication Number 2003/0166197 and WO 2000/32761; (F) For plant transcription factors or transcriptional regulators of abiotic stress, see, e.g., US Patent Application Publication Number 2004/0098764 or US Patent Application Publication Number 2004/0078852; (G) Genes that increase expression of vacuolar pyrophosphatase such as AVP1 (U.S. Pat. No. 8,058,515) for increased yield; nucleic acid encoding a HSFA4 or a HSFA5 (Heat Shock Factor of the class A4 or A5) polypeptides, an oligopeptide transporter protein (OPT4-like) polypeptide; a plastochron2-like (PLA2-like) polypeptide or a Wuschel related homeobox 1-like (WOX1-like) polypeptide (U. Patent Application Publication Number US 2011/0283420); (H) Down regulation of polynucleotides encoding poly (ADP-ribose) polymerase (PARP) proteins to modulate programmed cell death (U.S. Pat. No. 8,058,510) for increased vigor; (I) Polynucleotide encoding DTP21 polypeptides for conferring drought resistance (US Patent Application Publication Number US 2011/0277181); (J) Nucleotide sequences encoding ACC Synthase 3 (ACS3) proteins for modulating development, modulating response to stress, and modulating stress tolerance (US Patent Application Publication Number US 2010/0287669); (K) Polynucleotides that encode proteins that confer a drought tolerance phenotype (DTP) for conferring drought resistance (WO 2012/058528); (L) Tocopherol cyclase (TC) genes for conferring drought and salt tolerance (US Patent Application Publication Number 2012/0272352); (M) CAAX amino terminal family proteins for stress tolerance (U.S. Pat. No. 8,338,661); (N) Mutations in the SALT encoding gene have increased stress tolerance, including increased drought resistant (US Patent Application Publication Number 2010/0257633); (O) Expression of a nucleic acid sequence encoding a polypeptide selected from the group consisting of: GRF polypeptide, RAA1-like polypeptide, SYR polypeptide, ARKL polypeptide, and YTP polypeptide increasing yield-related traits (US Patent Application Publication Number 2011/0061133); and (P) Modulating expression in a plant of a nucleic acid encoding a Class III Trehalose Phosphate Phosphatase (TPP) polypeptide for enhancing yield-related traits in plants, particularly increasing seed yield (US Patent Application Publication Number 2010/0024067).
[0209] Other genes and transcription factors that affect plant growth and agronomic traits such as yield, flowering, plant growth and/or plant structure, can be introduced or introgressed into plants, see e.g., WO 1997/49811 (LHY), WO 1998/56918 (ESD4), WO 1997/10339 and U.S. Pat. No. 6,573,430 (TFL), U.S. Pat. No. 6,713,663 (FT), WO 1996/14414 (CON), WO 1996/38560, WO 2001/21822 (VRN1), WO 2000/44918 (VRN2), WO 1999/49064 (GI), WO 2000/46358 (FR1), WO 1997/29123, U.S. Pat. No. 6,794,560, U.S. Pat. No. 6,307,126 (GAI), WO 1999/09174 (D8 and Rht) and WO 2004/076638 and WO 2004/031349 (transcription factors).
[0210] vii. Genes that Confer Increased Yield
[0211] Non-limiting examples of genes that confer increased yield are: (A) A transgenic crop plant transformed by a 1-AminoCyclopropane-1-Carboxylate Deaminase-like Polypeptide (ACCDP) coding nucleic acid, wherein expression of the nucleic acid sequence in the crop plant results in the plant's increased root growth, and/or increased yield, and/or increased tolerance to environmental stress as compared to a wild type variety of the plant (U.S. Pat. No. 8,097,769); (B) Over-expression of maize zinc finger protein gene (Zm-ZFP1) using a seed preferred promoter has been shown to enhance plant growth, increase kernel number and total kernel weight per plant (US Patent Application Publication Number 2012/0079623); (C) Constitutive over-expression of maize lateral organ boundaries (LOB) domain protein (Zm-LOBDP1) has been shown to increase kernel number and total kernel weight per plant (US Patent Application Publication Number 2012/0079622); (D) Enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a VIM1 (Variant in Methylation 1)-like polypeptide or a VTC2-like (GDP-L-galactose phosphorylase) polypeptide or a DUF1685 polypeptide or an ARF6-like (Auxin Responsive Factor) polypeptide (WO 2012/038893); (E) Modulating expression in a plant of a nucleic acid encoding a Ste20-like polypeptide or a homologue thereof gives plants having increased yield relative to control plants (EP 2431472); and (F) Genes encoding nucleoside diphosphatase kinase (NDK) polypeptides and homologs thereof for modifying the plant's root architecture (US Patent Application Publication Number 2009/0064373).
IX. Methods of Use
[0212] Methods disclosed herein comprise methods for controlling a plant insect pest, such as a Coleopteran, Hemiptera, or Lepidopteran plant pest, including a Diabrotica, Leptinotarsa, Phyllotreta, Acyrthosiphan, Bemisia, Halyomorpha, Nezara, or Spodoptera plant pest. In one embodiment, the method comprises feeding or applying to a plant insect pest a composition comprising a silencing element disclosed herein, wherein said silencing element, when ingested or contacted by a plant insect pest (i.e., but not limited to, a Coleopteran plant pest including a Diabrotica plant pest, such as, D. virgifera virgifera, D. barberi, D. virgifera zeae, D. speciosa, or D. undecimpunctata howardi), reduces the level of a target polynucleotide of the pest and thereby controls the pest. The pest can be fed the silencing element in a variety of ways. The silencing element may be fed to male, female, or both sexes of a pest. For example, in an embodiment, a polynucleotide encoding a silencing element, i.e., a silencing element targeting one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, is introduced into a plant. As the plant pest feeds on the plant or part thereof expressing these sequences, the silencing element is delivered to the pest at larval, adult, or at any or all developmental stages. In one embodiment, the methods and compositions described herein further comprise a transgenic plant comprising a silencing element disclosed herein, wherein the silencing element has sterilization activity at larval, adult or at any or all developmental stages. When the silencing element is delivered to the plant in this manner, it is recognized that the silencing element can be expressed constitutively or alternatively, it may be produced in a stage-specific manner by employing the various inducible or tissue-preferred or developmentally regulated promoters that are discussed elsewhere herein. In certain embodiments, the silencing element is expressed in the roots, stalk or stem, leaf including pedicel, xylem and phloem, fruit or reproductive tissue, silk, flowers and all parts therein or any combination thereof. Sterile insects may result from exposure to silencing elements in this manner and hence sterilize insects of opposite the sex through competitive mating or SIT.
[0213] In another method, a composition comprising at least one silencing element disclosed herein is applied to a plant. In such embodiments, the silencing element may be formulated in an agronomically suitable and/or environmentally acceptable carrier, which is preferably, suitable for dispersal in fields. In some embodiments, silencing elements targeting different insect stages, pathways, and sexes may be combined for sterility and insecticidal activities. In one embodiment, the silencing elements disclosed herein may be mixed with pesticidal chemicals by tank mix. In addition, the carrier may also include compounds that increase the half-life of the composition. In certain embodiments, the composition comprising the silencing element is formulated in such a manner such that it persists in the environment for a length of time sufficient to allow it to be delivered to a plant insect pest. In such embodiments, the composition can be applied to an area inhabited by a plant insect pest. In one embodiment, the composition is applied externally to a plant (i.e., by spraying a field) to protect the plant from pests. Sterile insects that result from exposure to silencing elements may sterilize insects of opposite sex through competitive mating or SIT.
[0214] In certain embodiments, the disclosed polynucleotides or constructs can be stacked with any combination of polynucleotide sequences of interest in order to create plants with a desired trait. A trait, as used herein, refers to the phenotype derived from a particular sequence or groups of sequences. For example, the polynucleotides described herein may be stacked with any other polynucleotides encoding polypeptides having pesticidal and/or insecticidal activity, such as other Bacillus thuringiensis toxic proteins (described in U.S. Pat. Nos. 5,366,892; 5,747,450; 5,737,514; 5,723,756; 5,593,881; and Geiser et al. (1986) Gene 48:109), lectins (Van Damme et al. (1994) Plant Mol. Biol. 24:825, pentin (described in U.S. Pat. No. 5,981,722), and the like. The combinations generated may also include multiple copies of any one of the polynucleotides of interest. The polynucleotides described herein can also be stacked with any other gene or combination of genes to produce plants with a variety of desired trait combinations including, but not limited to, traits desirable for animal feed such as high oil genes (e.g., U.S. Pat. No. 6,232,529); balanced amino acids (e.g., hordothionins (U.S. Pat. Nos. 5,990,389; 5,885,801; 5,885,802; and 5,703,409); barley high lysine (Williamson et al. (1987) Eur. J. Biochem. 165:99-106; and WO 98/20122) and high methionine proteins (Pedersen et al. (1986) J. Biol. Chem. 261:6279; Kirihara et al. (1988) Gene 71:359; and Musumura et al. (1989) Plant Mol. Biol. 12:123)); increased digestibility (e.g., modified storage proteins (U.S. application Ser. No. 10/053,410, filed Nov. 7, 2001); and thioredoxins (U.S. application Ser. No. 10/005,429, filed Dec. 3, 2001)).
[0215] Disclosed polynucleotides can also be stacked with traits desirable for disease or herbicide resistance (e.g., fumonisin detoxification genes (U.S. Pat. No. 5,792,931); avirulence and disease resistance genes (Jones et al. (1994) Science 266:789; Martin et al. (1993) Science 262:1432; Mindrinos et al. (1994) Cell 78:1089); acetolactate synthase (ALS) mutants that lead to herbicide resistance such as the S4 and/or Hra mutations; inhibitors of glutamine synthase such as phosphinothricin or basta (e.g., bar gene); and glyphosate resistance (EPSPS gene)); and traits desirable for processing or process products such as high oil (e.g., U.S. Pat. No. 6,232,529); modified oils (e.g., fatty acid desaturase genes (U.S. Pat. No. 5,952,544; WO 94/11516)); modified starches (e.g., ADPG pyrophosphorylases (AGPase), starch synthases (SS), starch branching enzymes (SBE), and starch debranching enzymes (SDBE)); and polymers or bioplastics (e.g., U.S. Pat. No. 5,602,321; beta-ketothiolase, polyhydroxybutyrate synthase, and acetoacetyl-CoA reductase (Schubert et al. (1988) J. Bacteriol. 170:5837-5847) facilitate expression of polyhydroxyalkanoates (PHAs)); the disclosures of which are herein incorporated by reference. One could also combine the polynucleotides with polynucleotides providing agronomic traits such as male sterility (e.g., see U.S. Pat. No. 5,583,210), stalk strength, drought resistance (e.g., U.S. Pat. No. 7,786,353), flowering time, or transformation technology traits such as cell cycle regulation or gene targeting (e.g., WO 99/61619, WO 00/17364, and WO 99/25821).
[0216] These stacked combinations can be created by any method including, but not limited to, cross-breeding plants by any conventional or TopCross methodology, or genetic transformation. If the sequences are stacked by genetically transforming the plants (i.e., molecular stacks), the polynucleotide sequences of interest can be combined at any time and in any order. For example, a transgenic plant comprising one or more desired traits can be used as the target to introduce further traits by subsequent transformation. The traits can be introduced simultaneously in a co-transformation protocol with the polynucleotides of interest provided by any combination of transformation cassettes. For example, if two sequences will be introduced, the two sequences can be contained in separate transformation cassettes (trans) or contained on the same transformation cassette (cis). Expression of the sequences can be driven by the same promoter or by different promoters. In certain cases, it may be desirable to introduce a transformation cassette that will suppress the expression of the polynucleotide of interest. This may be combined with any combination of other suppression cassettes or overexpression cassettes to generate the desired combination of traits in the plant. It is further recognized that polynucleotide sequences can be stacked at a desired genomic location using a site-specific recombination system. See, for example, WO99/25821, WO99/25854, WO99/25840, WO99/25855, and WO99/25853.
X. Insect Resistance Management Methods
[0217] Methods disclosed herein comprise methods for controlling a plant insect pest, such as a Coleopteran, Hemiptera, or Lepidopteran plant pest, including a Diabrotica, Leptinotarsa, Phyllotreta, Acyrthosiphan, Bemisia, Halyomorpha, Nezara, or Spodoptera plant pest, such as insect resistance management. Insect resistance management (IRM) is the term used to describe practices aimed at reducing the potential for insect pests to become resistant to a pesticide. Maintenance of Bt (or other pesticidal protein, chemical, or biological) IRM is of great importance because of the threat insect resistance poses to the future use of Bt plant-incorporated protectants and Bt technology as a whole. Specific IRM strategies, such as the high dose/structured refuge strategy, delay insect resistance to specific Bt proteins produced in corn, cotton, and potatoes. However, such strategies result in portions of crops being left susceptible to one or more pests in order to ensure that non-resistant insects develop and become available to mate with any resistant pests produced in protected crops. Accordingly, from a farmer/producer's perspective, it is highly desirable to have as small a refuge as possible and yet still manage insect resistance, in order that the greatest yield be obtained while still maintaining the efficacy of the pest control method used, whether Bt, chemical, some other method, or combinations thereof.
[0218] An often used IRM strategy is the planting of a refuge (a portion of the total acreage using non-Bt/pesticidal trait seed), as it is commonly-believed that this will delay the development of insect resistance to pesticidal traits by maintaining insect susceptibility. The theoretical basis of the refuge strategy for delaying resistance hinges on the assumption that the frequency and recessiveness of insect resistance is inversely proportional to pest susceptibility; resistance will be rare and recessive only when pests are very susceptible to the toxin, and conversely resistance will be more frequent and less recessive when pests are not very susceptible. Furthermore, the strategy assumes that resistance to Bt is recessive and is conferred by a single locus with two alleles resulting in three genotypes: susceptible homozygotes (SS), heterozygotes (RS), and resistant homozygotes (RR). It also assumes that there will be a low initial resistance allele frequency and that there will be extensive random mating between resistant and susceptible adults. Under ideal circumstances, only rare RR individuals will survive a pesticidal toxin produced by the crop. Both SS and RS individuals will be susceptible to the pesticidal toxin. A structured refuge is a non-Bt/pesticidal trait portion of a grower's field or set of fields that provides for the production of susceptible (SS) insects that may randomly mate with rare resistant (RR) insects surviving the pesticidal trait crop, which may be a Bt trait crop, to produce susceptible RS heterozygotes that will be killed by the Bt/pesticidal trait crop. An integrated refuge is a certain portion of randomly planted non-Bt/pesticidal trait portion of a grower's field or set of fields that provides for the production of susceptible (SS) insects that may randomly mate with rare resistant (RR) insects surviving the pesticidal trait crop to produce susceptible RS heterozygotes that will be killed by the pesticidal trait crop Each refuge strategy will remove resistant (R) alleles from the insect populations and delay the evolution of resistance.
[0219] Another strategy to reduce the need for refuge is the pyramiding of traits with different modes of action against a target insect pest. For example, Bt toxins that have different modes of action stacked in one transgenic plant are able to have reduced refuge requirements. Different modes of action in a stacked combination also maintains the durability of each trait, as resistance is slower to develop to each trait.
[0220] Currently, the size, placement, and management of the refuge are often considered critical to the success of refuge strategies to mitigate insect resistance to the Bt/pesticidal trait produced in corn, cotton, soybean, and other crops. Because of the decrease in yield in refuge planting areas, some farmers choose to eschew the refuge requirements, and others do not follow the size and/or placement requirements. These issues result in either no refuge or less effective refuge, and a corresponding risk of the increase in the development of resistance pests.
[0221] Accordingly, there remains a need for methods for managing pest resistance in a plot of pest resistant crop plants. It would be useful to provide an improved method for the protection of plants, especially corn or other crop plants, from feeding damage by pests. It would be particularly useful if such a method would reduce the required application rate of conventional chemical pesticides, and also if it would limit the number of separate field operations that were required for crop planting and cultivation. In addition, it would be useful to have a method of deploying a transgenic refuge that eliminates the above-described problems with regard to compliance that dilute or remove the efficacy of many resistance management strategies.
[0222] One embodiment relates to a method of reducing the development of resistant pests comprising providing a plant protection composition to a plant (Bt toxin, transgenic insecticidal protein, other insecticidal proteins, chemical insecticides, insecticidal biological entomopathogens, etc.) and contacting the plant pest with a silencing element, i.e., of a silencing element targeting one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, wherein the silencing element, i.e., of a silencing element targeting one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, produces a decrease in expression of one or more of the sequences in the target pest and controls the pest and pest population by insect sterilization or SIT.
[0223] A further embodiment relates to a method of increasing the durability of plant pest compositions comprising providing a plant protection composition to a plant (Bt toxin, transgenic insecticidal protein, other insecticidal proteins, chemical insecticides, insecticidal biological entomopathogens etc.) and contacting a plant pest with the sterilization silencing element, i.e., of one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or silencing elements targeting said polynucleotides, produces a decrease in expression of one or more of the sequences in the target pest and controls the pest and pest population by insect sterilization or sterile insect technique. In another embodiment, the refuge planted as a strip, a block, or integrated with the trait seed comprises a plant further comprising a sterilization silencing element (for example, a silencing element targeting one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254).
[0224] In a still further embodiment, the refuge required may be reduced or eliminated by the presence of a sterilization silencing element applied to the non-refuge plants. In another embodiment, the refuge or non-refuge may include a silencing element, i.e., of one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or silencing elements targeting said polynucleotides, as a spray, bait, lure, or as a different transgenic plant.
[0225] In a further embodiment, a pest insect is feed a diet comprising one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or silencing elements targeting said polynucleotides, and said insects are released onto plants at 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 days following feeding. In a still further embodiment, the pest insect is a female pest insect. In a yet further embodiment, the pest insect is a pest insect, and the pest insect is fed during a larval or adult stage. Insect sterilization may result from male or female sterility, mating of sterile insects, reduction of sperm count, egg production or viability.
[0226] In certain embodiments, the compositions and methods disclosed herein, targeting a sterile gene via RNAi technology, and stacking a polynucleotide encoding a silencing element disclosed herein with an insecticidal protein in a transgenic plant may provide effective control of Coleoptera and potentially extend the durability of Coleopteran insecticidal traits. The extended durability may be a consequence of minimizing the transmission of resistance alleles from Coleopteran beetles that were able to complete their developmental life cycle while feeding on transgenic roots expressing a stack of an insecticidal protein(s) and a RNAi sterility trait disclosed herein.
[0227] Current IRM strategy requires a high dose of Bt toxins to minimize insect resistance development. Due to phyto-toxicity, it can be difficult to achieve the required high dose. Integrated pest management (IPM) by different means of insect control may be used to delay insect resistance exposed to a sub-optimal dose of protein toxin, such as a Bt toxin. RNAi mediated SIT may be deployed as part of an IPM strategy.
[0228] As used herein, the term "pesticidal" is used to refer to a toxic effect against a pest (e.g., CRW), and includes activity of either, or both, an externally supplied pesticide and/or an agent that is produced by the crop plants. As used herein, the term "different mode of pesticidal action" includes the pesticidal effects of one or more resistance traits, whether introduced into the crop plants by transformation or traditional breeding methods, such as binding of a pesticidal toxin produced by the crop plants to different binding sites (i.e., different toxin receptors and/or different sites on the same toxin receptor) in the gut membranes of corn rootworms or through RNA interference.
XI. Application Methods
[0229] In one embodiment, one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or silencing elements targeting said polynucleotide sequences, and compositions comprising said sequences can be applied directly to the seed. For example, one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or silencing elements targeting said polynucleotide sequences, used in the compositions and methods disclosed herein can be applied without additional components and without having been diluted.
[0230] In one embodiment, sprays, baits, lures, attractants, and seed treatments can comprise one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or silencing elements targeting said polynucleotide sequences, and compositions comprising said sequences.
[0231] In another embodiment, one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or silencing elements targeting said polynucleotide sequences, and compositions comprising said sequences are applied to the seed in the form of a suitable formulation. Suitable formulations and methods for the treatment of seed are known to the person skilled in the art and are described, for example, in the following documents: U.S. Pat. No. 4,272,417 A, U.S. Pat. No. 4,245,432 A, U.S. Pat. No. 4,808,430 A, U.S. Pat. No. 5,876,739 A, US 2003/0176428 A1, WO 2002/080675 A1, WO 2002/028186 A2.
[0232] The one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or silencing elements targeting said polynucleotide sequences, and compositions comprising said sequences can be converted into customary seed dressing formulations, such as solutions, emulsions, suspensions, powders, foams, slurries or other coating materials for seed, and also ULV formulations. These formulations are prepared in a known manner by mixing the one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or silencing elements targeting said polynucleotide sequences, and compositions comprising said sequences with customary additives, such as, for example, customary extenders and also solvents or diluents, colorants, wetting agents, dispersants, emulsifiers, defoamers, preservatives, secondary thickeners, adhesives, gibberellins and water as well.
[0233] In another embodiment, suitable colorants that may be present in the seed dressing formulations include all colorants customary for such purposes. Use may be made both of pigments, of sparing solubility in water, and of dyes, which are soluble in water. Examples that may be mentioned include the colorants known under the designations Rhodamine B, C.I. Pigment Red 112, and C.I. Solvent Red 1
[0234] In another embodiment, suitable wetting agents that may be present in the seed dressing formulations include all substances that promote wetting and are customary in the formulation of active agrochemical substances. With preference it is possible to use alkylnaphthalene-sulphonates, such as diisopropyl- or diisobutylnaphthalene-sulphonates.
[0235] In still another embodiment, suitable dispersants and/or emulsifiers that may be present in the seed dressing formulations include all nonionic, anionic, and cationic dispersants that are customary in the formulation of active agrochemical substances. In one embodiment, nonionic or anionic dispersants or mixtures of nonionic or anionic dispersants can be used. In one embodiment, nonionic dispersants include but are not limited to ethylene oxide-propylene oxide block polymers, alkylphenol polyglycol ethers, and tristyrylphenol polyglycol ethers, and their phosphated or sulphated derivatives.
[0236] In still another embodiment, defoamers that may be present in the seed dressing formulations to be used according to the invention include all foam-inhibiting compounds that are customary in the formulation of agrochemically active compounds including, but not limited, to silicone defoamers, magnesium stearate, silicone emulsions, long-chain alcohols, fatty acids and their salts and also organofluorine compounds and mixtures thereof.
[0237] In still another embodiment, secondary thickeners that may be present in the seed dressing formulations include all compounds which can be used for such purposes in agrochemical compositions, including but not limited to cellulose derivatives, acrylic acid derivatives, polysaccharides, such as xanthan gum or Veegum, modified clays, phyllosilicates, such as attapulgite and bentonite, and also finely divided silicic acids.
[0238] Suitable adhesives that may be present in the seed dressing formulations to be used according to the invention include all customary binders which can be used in seed dressings. Polyvinylpyrrolidone, polyvinyl acetate, polyvinyl alcohol and tylose may be mentioned as being preferred.
[0239] In another embodiment, one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or silencing elements targeting said polynucleotide sequences, and compositions comprising said sequences is applied to soil in a first application step, applied to seed in a second application, and to applied to the foliar region of a plant in a third application.
[0240] As used herein, applying one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or a complement thereof, an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or a complement thereof, or silencing elements targeting said polynucleotide sequences, and compositions comprising said sequences to a seed, a plant, or plant part includes contacting the seed, plant, or plant part directly and/or indirectly with the one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or silencing elements targeting said polynucleotide sequences, and compositions comprising said sequences. In one embodiment, one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or silencing elements targeting said polynucleotide sequences, and compositions comprising said sequences can be directly applied as a spray, a rinse, or a powder, or any combination thereof.
[0241] In another aspect, one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or silencing elements targeting said polynucleotide sequences, and compositions comprising said sequences can be applied directly to a plant or plant part as a powder. As used herein, a powder is a dry or nearly dry bulk solid composed of a large number of very fine particles that may flow freely when shaken or tilted. A dry or nearly dry powder composition disclosed herein preferably contains a low percentage of water, such as, for example, in various aspects, less than 5%, less than 2.5%, or less than 1% by weight.
[0242] In a further embodiment, one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or silencing elements targeting said polynucleotide sequences, may be introduced in a bacteria, a yeast, or fungus by transformation techniques known to the skilled artisan, and said transformed bacteria, yeast, or fungus applied to a plant, soil that the plant is growing in, to a hydroponic medium, seed, or any applied per any of the foregoing application methods as described herein above.
[0243] In one embodiment, the one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or silencing elements targeting said polynucleotide sequences, and compositions comprising said sequences may be formulated by encapsulation technology to improve stability. In one embodiment the encapsulation technology may comprise a bead polymer for timed release over time. In one embodiment, the encapsulated one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or silencing elements targeting said polynucleotide sequences, and compositions comprising said sequences may be applied in a separate application of beads in-furrow to the seeds. In another embodiment, the encapsulated one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or silencing elements targeting said polynucleotide sequences, and compositions comprising said sequences may be co-applied along with seeds simultaneously.
[0244] The coating agent usable for the sustained release microparticles of an encapsulation embodiment may be a substance which is useful for coating the microgranular form with the substance to be supported thereon. Any coating agent which can form a coating difficulty permeable for the supported substance may be used in general, without any particular limitation. For example, higher saturated fatty acid, wax, thermoplastic resin, thermosetting resin and the like may be used.
[0245] Examples of useful higher saturated fatty acid include stearic acid, zinc stearate, stearic acid amide and ethylenebis-stearic acid amide; those of wax include synthetic waxes such as polyethylene wax, carbon wax, Hoechst wax, and fatty acid ester; natural waxes such as carnauba wax, bees wax and Japan wax; and petroleum waxes such as paraffin wax and petrolatum. Examples of thermoplastic resin include polyolefins such as polyethylene, polypropylene, polybutene and polystyrene; vinyl polymers such as polyvinyl acetate, polyvinyl chloride, polyvinylidene chloride, polyacrylic acid, polymethacrylic acid, polyacrylate and polymethacrylate; diene polymers such as butadiene polymer, isoprene polymer, chloroprene polymer, butadiene-styrene copolymer, ethylene-propylene-diene copolymer, styrene-isoprene copolymer, MMA-butadiene copolymer and acrylonitrile-butadiene copolymer; polyolefin copolymers such as ethylene-propylene copolymer, butene-ethylene copolymer, butene-propylene copolymer, ethylene-vinyl acetate copolymer, ethylene-acrylic acid copolymer, styreneacrylic acid copolymer, ethylene-methacrylic acid copolymer, ethylene-methacrylic ester copolymer, ethylene-carbon monoxide copolymer, ethylene-vinyl acetate-carbon monoxide copolymer, ethylene-vinyl acetate-vinyl chloride copolymer and ethylene-vinyl acetate-acrylic copolymer; and vinyl chloride copolymers such as vinyl chloride-vinyl acetate copolymer and vinylidene chloride-vinyl chloride copolymer. Examples of thermosetting resin include polyurethane resin, epoxy resin, alkyd resin, unsaturated polyester resin, phenolic resin, urea-melamine resin, urea resin and silicone resin. Of those, thermoplastic acrylic ester resin, butadienestyrene copolymer resin, thermosetting polyurethane resin and epoxy resin are preferred, and among the preferred resins, particularly thermosetting polyurethane resin is preferred. These coating agents can be used either singly or in combination of two or more kinds.
[0246] In one embodiment, one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or silencing elements targeting said polynucleotide sequences, and compositions comprising said sequences can be formulated to further comprise an entomopathogen. The methods and compositions of the disclosure, in one embodiment relate to a composition comprising one or more one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or silencing elements targeting said polynucleotide sequences, and compositions comprising said sequences and one or more biocontrol agents. As used herein, the term "biocontrol agent" ("BCA") includes one or more bacteria, fungi or yeasts, protozoas, viruses, entomopathogenic nematodes, and botanical extracts, or products produced by microorganisms including proteins or secondary metabolite, and innoculants that have one or both of the following characteristics: (1) inhibits or reduces plant infestation and/or growth of pathogens, pests, or insects, including but not limited to pathogenic fungi, bacteria, and nematodes, as well as arthropod pests such as insects, arachnids, chilopods, diplopods, or that inhibits plant infestation and/or growth of a combination of plant pathogens, pests, or insects; (2) improves plant performance; (3) improves plant yield; (4) improves plant vigor; and (5) improves plant health.
XII. Knockout of Target Genes Using Cas/CRISPR
[0247] In one embodiment, one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, an expression construct comprising a sequence as set forth in SEQ ID NOS.: 1-53 or 107-254, or complements thereof, or silencing elements targeting said polynucleotides, and compositions comprising said sequences, can be can be introduced into the genome of a plant using genome editing technologies, or previously introduced polynucleotides encoding a silencing element disclosed herein in the genome of a plant may be edited using genome editing technologies. For example, the disclosed polynucleotides can be introduced into a desired location in the genome of a plant through the use of double-stranded break technologies such as TALENs, meganucleases, zinc finger nucleases, CRISPR-Cas, and the like. For example, the disclosed polynucleotides can be introduced into a desired location in a genome using a CRISPR-Cas system, for the purpose of site-specific insertion. The desired location in a plant genome can be any desired target site for insertion, such as a genomic region amenable for breeding or may be a target site located in a genomic window with an existing trait of interest. Existing traits of interest could be either an endogenous trait or a previously introduced trait.
[0248] In another aspect, where the disclosed polynucleotide encoding a silencing element has previously been introduced into a genome, genome editing technologies may be used to alter or modify the introduced polynucleotide sequence. Site specific modifications that can be introduced into the disclosed polynucleotide encoding a silencing element compositions include those produced using any method for introducing site specific modification, including, but not limited to, through the use of gene repair oligonucleotides (e.g. US Publication 2013/0019349), or through the use of double-stranded break technologies such as TALENs, meganucleases, zinc finger nucleases, CRISPR-Cas, and the like. Such technologies can be used to modify the previously introduced polynucleotide through the insertion, deletion or substitution of nucleotides within the introduced polynucleotide.
[0249] Alternatively, double-stranded break technologies can be used to add additional nucleotide sequences to the introduced polynucleotide. Additional sequences that may be added include, additional expression elements, such as enhancer and promoter sequences. In another embodiment, genome editing technologies may be used to position additional insecticidally-active proteins in close proximity to the disclosed polynucleotide compositions disclosed herein within the genome of a plant, in order to generate molecular stacks of insecticidally-active proteins. An "altered target site," "altered target sequence." "modified target site," and "modified target sequence" are used interchangeably herein and refer to a target sequence as disclosed herein that comprises at least one alteration when compared to non-altered target sequence. Such "alterations" include, for example: (i) replacement of at least one nucleotide, (ii) a deletion of at least one nucleotide, (iii) an insertion of at least one nucleotide, or (iv) any combination of (i)-(iii).
[0250] In one embodiment, the methods comprise creating an insect, or colony thereof, wherein the target gene is edited so that it is no longer function, thereby creating a sterile insect. The polynucleotide sequence of the target gene can be used to knockout the target gene polynucleotide in an insect by means known to those skilled in the art, including, but not limited to TALENs, meganucleases, zinc finger nucleases, CRISPR-Cas, and the like. See Ma et al (2014), Scientific Reports, 4: 4489; Daimon et al (2013), Development, Growth, and Differentiation, 56(1): 14-25; and Eggleston et al (2001) BMC Genetics, 2:11. One embodiment comprises an insect with an edited polynucleotide of one or more polynucleotides as set forth in SEQ ID NOS.: 1-53 wherein the edit produces a decrease in expression of or a nonfunctional polypeptide and controls the pest and pest population by insect sterilization and sterile insect technique.
[0251] All publications and patent applications mentioned in the specification are indicative of the level of those skilled in the art to which this invention pertains. All publications and patent applications are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference.
[0252] Although the foregoing embodiments have been described in some detail by way of illustration and example for purposes of clarity of understanding, certain changes and modifications may be practiced within the scope of the appended claims.
[0253] The following examples are offered by way of illustration and not by way of limitation.
EXPERIMENTAL
Example 1: Nucleic Acid Sequences
[0254] Nucleic acid sequences disclosed herein comprise the following nucleic acid sequences. Certain sequences are exemplary and were shown to have insect sterilization activity against corn rootworms using the assay methods described in Examples 2, 3, and 6 as set forth below. Such sequences or their complements can be used in the methods as described herein above and below. Methods for making inhibitory sequences are known in the art. DNA constructs, vectors, transgenic cells, plants, seeds or products described herein may comprise one or more of the following nucleic acid or amino acid sequences, or a portion of one or more of the disclosed sequences. Non-limiting examples of target polynucleotides are set forth below in Table 1, or variants and fragments thereof, and complements thereof, including, for example, SEQ ID NOS.: 1-53 or 107-254, and variants and fragments thereof, and complements thereof. The list of sequences referred to herein, SEQ ID NOS.: 1-53 and 107-254, is included herein below.
TABLE-US-00001 TABLE 1 VgR RNAi target fragments. SEQ ID NO. Species Common name Fragment ID 1 Diabrotica virgifera Western Corn Rootworm Transcript* virgifera 2 Diabrotica virgifera Western Corn Rootworm ORF** virgifera 3 Diabrotica virgifera Western Corn Rootworm DV-VGR-Frag1 virgifera 4 Diabrotica virgifera Western Corn Rootworm DV-VGR-Frag2 virgifera 5 Diabrotica virgifera Western Corn Rootworm DV-VGR-Frag3 virgifera 6 Diabrotica virgifera Western Corn Rootworm DV-VGR-Frag4 virgifera 7 Diabrotica virgifera Western Corn Rootworm DV-VGR-Frag5 virgifera 8 Diabrotica virgifera Western Corn Rootworm DV-VGR-Frag6 virgifera 9 Diabrotica virgifera Western Corn Rootworm DV-VGR-Frag7 virgifera 10 Diabrotica virgifera Western Corn Rootworm DV-VGR-Frag8 virgifera 11 Diabrotica virgifera Western Corn Rootworm DV-VGR-Frag9 virgifera 12 Diabrotica virgifera Western Corn Rootworm DV-VGR-Frag10 virgifera 13 Diabrotica virgifera Western Corn Rootworm DV-VGR-Frag11 virgifera 14 Diabrotica virgifera Western Corn Rootworm DV-VGR-Frag12 virgifera 15 Diabrotica virgifera Western Corn Rootworm DV-VGR-Frag13 virgifera 16 Diabrotica virgifera Western Corn Rootworm DV-VGR-Frag14 virgifera 17 Diabrotica virgifera Western Corn Rootworm DV-VGR-Frag15 virgifera 18 Diabrotica virgifera Western Corn Rootworm DV-VGR-Frag16 virgifera 19 Diabrotica Southern Corn Rootworm ORF undecimpunctata 20 Diabrotica Southern Corn Rootworm Transcript undecimpunctata 21 Leptinotarsa Colorado Potato Beetle ORF decemlineata 22 Leptinotarsa Colorado Potato Beetle Transcript decemlineata 23 Phyllotreta striolata Striped Flea Beetle ORF 24 Phyllotreta striolata Striped Flea Beetle Transcript 25 Halyomorpha halys Brown Marmorated Stink ORF Bug 26 Halyomorpha halys Brown Marmorated Stink Transcript Bug 27 Acyrthosiphon pisum Pea Aphid ORF 28 Acyrthosiphon pisum Pea Aphid Transcript 29 Bemisia tabaci Silverleaf Whitefly ORF 30 Bemisia tabaci Silverleaf Whitefly Transcript 31 Spodoptera litura Cotton Leafworm ORF 32 Spodoptera litura Cotton Leafworm Transcript 33 Phyllotreta cruciferae Crucifer Flea Beetle ORF 34 Phyllotreta cruciferae Crucifer Flea Beetle Transcript 35 Nezara viridula Southern Green Stink Bug ORF 36 Diabrotica virgifera Western Corn Rootworm DV-CUL3 virgifera 37 Diabrotica virgifera Western Corn Rootworm DV-NCLB virgifera 38 Diabrotica virgifera Western Corn Rootworm DV-MAEL virgifera 39 Diabrotica virgifera Western Corn Rootworm DV-GUDU virgifera 40 Diabrotica virgifera Western Corn Rootworm DV-GSKT virgifera 41 Diabrotica virgifera Western Corn Rootworm DV-WTS virgifera 42 Diabrotica virgifera Western Corn Rootworm DV-CASP virgifera 43 Diabrotica virgifera Western Corn Rootworm DV-CYCA virgifera 44 Diabrotica virgifera Western Corn Rootworm DV-CUL3-FRAG1 virgifera 45 Diabrotica virgifera Western Corn Rootworm DV-NCLB-FRAG1 virgifera 46 Diabrotica virgifera Western Corn Rootworm DV-MAEL-FRAG1 virgifera 47 Diabrotica virgifera Western Corn Rootworm DV-GUDU-FRAG1 virgifera 48 Diabrotica virgifera Western Corn Rootworm DV-GSKT-FRAG1 virgifera 49 Diabrotica virgifera Western Corn Rootworm DV-WTS-FRAG1 virgifera 50 Diabrotica virgifera Western Corn Rootworm DV-CASP-FRAG1 virgifera 51 Diabrotica virgifera Western Corn Rootworm DV-CYCA-FRAG1 virgifera 52 Spodoptera frugiperda Fall Armyworm ORF 53 Spodoptera frugiperda Fall Armyworm Transcript 107 Diabrotica virgifera Western Corn Rootworm DV-ADE2 virgifera 108 Diabrotica virgifera Western Corn Rootworm DV-HANG virgifera 109 Diabrotica virgifera Western Corn Rootworm DV-KL3 virgifera 110 Diabrotica virgifera Western Corn Rootworm DV-PORIN virgifera 111 Diabrotica virgifera Western Corn Rootworm DV-SU(VAR)205 virgifera 112 Diabrotica virgifera Western Corn Rootworm DV-PARK virgifera 113 Diabrotica virgifera Western Corn Rootworm DV-POE virgifera 114 Diabrotica virgifera Western Corn Rootworm DV-MBD-LIKE virgifera 115 Diabrotica virgifera Western Corn Rootworm DV-PGLYM78 virgifera 116 Diabrotica virgifera Western Corn Rootworm DV-HIRA virgifera 117 Diabrotica virgifera Western Corn Rootworm DV-PUF virgifera 118 Diabrotica virgifera Western Corn Rootworm DV-TUD virgifera 119 Diabrotica virgifera Western Corn Rootworm DV-FAF virgifera 120 Diabrotica virgifera Western Corn Rootworm DV-NUP44A virgifera 121 Diabrotica virgifera Western Corn Rootworm DV-GEK virgifera 122 Diabrotica virgifera Western Corn Rootworm DV-HTS virgifera 123 Diabrotica virgifera Western Corn Rootworm DV-CDK7 virgifera 124 Diabrotica virgifera Western Corn Rootworm DV-DLG1 virgifera 125 Diabrotica virgifera Western Corn Rootworm DV-DM virgifera 126 Diabrotica virgifera Western Corn Rootworm DV-EGG virgifera 127 Diabrotica virgifera Western Corn Rootworm DV-HRG virgifera 128 Diabrotica virgifera Western Corn Rootworm DV-MR virgifera 129 Diabrotica virgifera Western Corn Rootworm DV-CG17083 virgifera 130 Diabrotica virgifera Western Corn Rootworm DV-CG3565 virgifera 131 Diabrotica virgifera Western Corn Rootworm DV-CYCB virgifera 132 Diabrotica virgifera Western Corn Rootworm DV-KNRL virgifera 133 Diabrotica virgifera Western Corn Rootworm DV-MEI virgifera 134 Diabrotica virgifera Western Corn Rootworm DV-TWE virgifera 135 Diabrotica virgifera Western Corn Rootworm DV-BOULE virgifera 136 Diabrotica virgifera Western Corn Rootworm DV-ADE2-FRAG1 virgifera 137 Diabrotica virgifera Western Corn Rootworm DV-HANG-FRAG1 virgifera 138 Diabrotica virgifera Western Corn Rootworm DV-KL3-FRAG1 virgifera 139 Diabrotica virgifera Western Corn Rootworm DV-PORIN-FRAG1 virgifera 140 Diabrotica virgifera Western Corn Rootworm DV-SU(VAR)205-FRAG1 virgifera 141 Diabrotica virgifera Western Corn Rootworm DV-PARK-FRAG1 virgifera 142 Diabrotica virgifera Western Corn Rootworm DV-POE-FRAG1 virgifera 143 Diabrotica virgifera Western Corn Rootworm DV-MBD-LIKE-FRAG1 virgifera 144 Diabrotica virgifera Western Corn Rootworm DV-PGLYM78-FRAG1 virgifera 145 Diabrotica virgifera Western Corn Rootworm DV-HIRA-FRAG1 virgifera 146 Diabrotica virgifera Western Corn Rootworm DV-PUF-FRAG1 virgifera 147 Diabrotica virgifera Western Corn Rootworm DV-TUD-FRAG1 virgifera 148 Diabrotica virgifera Western Corn Rootworm DV-FAF-FRAG1 virgifera 149 Diabrotica virgifera Western Corn Rootworm DV-NUP44A-FRAG1 virgifera 150 Diabrotica virgifera Western Corn Rootworm DV-GEK-FRAG1 virgifera 151 Diabrotica virgifera Western Corn Rootworm DV-HTS-FRAG1 virgifera 152 Diabrotica virgifera Western Corn Rootworm DV-CDK7-FRAG1 virgifera 153 Diabrotica virgifera Western Corn Rootworm DV-DLG1-FRAG1 virgifera 154 Diabrotica virgifera Western Corn Rootworm DV-DM-FRAG1 virgifera 155 Diabrotica virgifera Western Corn Rootworm DV-EGG-FRAG1 virgifera 156 Diabrotica virgifera Western Corn Rootworm DV-HRG-FRAG1 virgifera 157 Diabrotica virgifera Western Corn Rootworm DV-MR-FRAG1 virgifera 158 Diabrotica virgifera Western Corn Rootworm DV-CG17083-FRAG1 virgifera 159 Diabrotica virgifera Western Corn Rootworm DV-CG3565-FRAG1 virgifera 160 Diabrotica virgifera Western Corn Rootworm DV-CYCB-FRAG1 virgifera 161 Diabrotica virgifera Western Corn Rootworm DV-KNRL-FRAG1 virgifera 162 Diabrotica virgifera Western Corn Rootworm DV-MEI-FRAG1 virgifera 163 Diabrotica virgifera Western Corn Rootworm DV-TWE-FRAG1 virgifera 164 Diabrotica virgifera Western Corn Rootworm DV-BOULE-FRAG1 virgifera 165 Diabrotica virgifera Western Corn Rootworm DV-REPH virgifera 166 Diabrotica virgifera Western Corn Rootworm DV-ARMI virgifera 167 Diabrotica virgifera Western Corn Rootworm DV-LOQS virgifera 168 Diabrotica virgifera Western Corn Rootworm DV-SCNY virgifera 169 Diabrotica virgifera Western Corn Rootworm DV-AGO3 virgifera 170 Diabrotica virgifera Western Corn Rootworm DV-DIA virgifera 171 Diabrotica virgifera Western Corn Rootworm DV-DNC virgifera 172 Diabrotica virgifera Western Corn Rootworm DV-CHI virgifera 173 Diabrotica virgifera Western Corn Rootworm DV-SXL virgifera 174 Diabrotica virgifera Western Corn Rootworm DV-SLGA virgifera 175 Diabrotica virgifera Western Corn Rootworm DV-PAPLA1 virgifera 176 Diabrotica virgifera Western Corn Rootworm DV-REPH-FRAG1 virgifera 177 Diabrotica virgifera Western Corn Rootworm DV-ARMI-FRAG1 virgifera 178 Diabrotica virgifera Western Corn Rootworm DV-LOQS-FRAG1 virgifera 179 Diabrotica virgifera Western Corn Rootworm DV-SCNY-FRAG1 virgifera 180 Diabrotica virgifera Western Corn Rootworm DV-AGO3-FRAG1 virgifera 181 Diabrotica virgifera Western Corn Rootworm DV-DIA-FRAG1 virgifera 182 Diabrotica virgifera Western Corn Rootworm DV-DNC-FRAG1
virgifera 183 Diabrotica virgifera Western Corn Rootworm DV-CHI-FRAG1 virgifera 184 Diabrotica virgifera Western Corn Rootworm DV-SXL-FRAG1 virgifera 185 Diabrotica virgifera Western Corn Rootworm DV-SLGA-FRAG1 virgifera 186 Diabrotica virgifera Western Corn Rootworm DV-PAPLA1-FRAG1 virgifera 187 Diabrotica Southern Corn Rootworm DU-BOULE undecimpunctata 188 Leptinotarsa Colorado Potato Beetle LD-BOULE decemlineata 189 Phyllotreta cruciferae Crucifer Flea Beetle PC-BOULE 190 Phyllotreta striolata Striped Flea Beetle PS-BOULE 191 Vibidia duodecimguttata 12-Spotted Ladybeetle VD-BOULE 192 Orius insidiosus Insidious Flower Bug OI-BOULE 193 Lygus hesperus Western Plant Bug LH-BOULE 194 Megacopta cribraria Kudzu Bug MC-BOULE 195 Euschistus servus Brown Stink Bug ES-BOULE 196 Nezara viridula Southern Green Stink Bug NV-BOULE 197 Helicoverpa zea Corn Earworm HZ-BOULE 198 Ostrinia nubilalis European Corn Borer ON-BOULE 199 Spodoptera frugiperda Fall Armyworm SF-BOULE 200 Diabrotica Southern Corn Rootworm DU-MAEL undecimpunctata 201 Leptinotarsa Colorado Potato Beetle LD-MAEL decemlineata 202 Phyllotreta striolata Striped Flea Beetle PS-MAEL 203 Phyllotreta cruciferae Crucifer Flea Beetle PC-MAEL 204 Epilachna varivestis Mexican Bean Beetle EV-MAEL 205 Tribolium castaneum Red Flour Beetle TC-MAEL 206 Vibidia duodecimguttata 12-Spotted Ladybeetle VD-MAEL 207 Helicoverpa zea Corn Earworm HZ-MAEL 208 Megacopta cribraria Kudzu Bug MC-MAEL 209 Nezara viridula Southern Green Stink Bug NV-MAEL 210 Euschistus servus Brown Stink Bug ES-MAEL 211 Orius insidiosus Insidious Flower Bug OI-MAEL 212 Manduca sexta Hornworm MS-MAEL 213 Spodoptera frugiperda Fall Armyworm SF-MAEL 214 Ostrinia nubilalis European Corn Borer ON-MAEL 215 Lygus hesperus Western Plant Bug LH-MAEL 216 Pectinophora gossypiella Pink Bollworm PG-MAEL 217 Diabrotica barberi Northern Corn Rootworm DB-NCLB 218 Diabrotica Southern Corn Rootworm DU-NCLB undecimpunctata 219 Phyllotreta striolata Striped Flea Beetle PS-NCLB 220 Phyllotreta cruciferae Crucifer Flea Beetle PC-NCLB 221 Leptinotarsa Colorado Potato Beetle LD-NCLB decemlineata 222 Tribolium castaneum Red Flour Beetle TC-NCLB 223 Epilachna varivestis Mexican Bean Beetle EV-NCLB 224 Vibidia duodecimguttata 12-Spotted Ladybeetle VD-NCLB 225 Pectinophora gossypiella Pink Bollworm PG-NCLB 226 Spodoptera frugiperda Fall Armyworm SF-NCLB 227 Ostrinia nubilalis European Corn Borer ON-NCLB 228 Manduca sexta Hornworm MS-NCLB 229 Helicoverpa zea Corn Earworm HZ-NCLB 230 Megacopta cribraria Kudzu Bug MC-NCLB 231 Nezara viridula Southern Green Stink Bug NV-NCLB 232 Diabrotica virgifera Western Corn Rootworm DV-BOULE-FRAG2 virgifera 233 Diabrotica virgifera Western Corn Rootworm DV-BOULE-FRAG3 virgifera 234 Diabrotica virgifera Western Corn Rootworm DV-BOULE-FRAG4 virgifera 235 Diabrotica virgifera Western Corn Rootworm DV-BOULE-FRAG5 virgifera 236 Diabrotica virgifera Western Corn Rootworm DV-BOULE-FRAG6 virgifera 237 Diabrotica virgifera Western Corn Rootworm DV-BOULE-FRAG7 virgifera 238 Diabrotica virgifera Western Corn Rootworm DV-BOULE-FRAG8 virgifera 239 Diabrotica virgifera Western Corn Rootworm DV-BOULE-FRAG9 virgifera 240 Diabrotica virgifera Western Corn Rootworm DV-MAEL-FRAG2 virgifera 241 Diabrotica virgifera Western Corn Rootworm DV-MAEL-FRAG3 virgifera 242 Diabrotica virgifera Western Corn Rootworm DV-MAEL-FRAG4 virgifera 243 Diabrotica virgifera Western Corn Rootworm DV-MAEL-FRAG5 virgifera 244 Diabrotica virgifera Western Corn Rootworm DV-MAEL-FRAG6 virgifera 245 Diabrotica virgifera Western Corn Rootworm DV-MAEL-FRAG7 virgifera 246 Diabrotica virgifera Western Corn Rootworm DV-MAEL-FRAG8 virgifera 247 Diabrotica virgifera Western Corn Rootworm DV-MAEL-FRAG9 virgifera 248 Diabrotica virgifera Western Corn Rootworm DV-MAEL-FRAG10 virgifera 249 Diabrotica virgifera Western Corn Rootworm DV-NCLB-FRAG2 virgifera 250 Diabrotica virgifera Western Corn Rootworm DV-NCLB-FRAG3 virgifera 251 Diabrotica virgifera Western Corn Rootworm DV-NCLB-FRAG4 virgifera 252 Diabrotica virgifera Western Corn Rootworm DV-NCLB-FRAG5 virgifera 253 Diabrotica virgifera Western Corn Rootworm DV-NCLB-FRAG6 virgifera 254 Diabrotica virgifera Western Corn Rootworm DV-RPS10 virgifera *"Transcipt" indicates that the sequence is a full-length mRNA transcript sequence. **"ORF" indicates that the sequence corresponds to the open reading frame sequence.
Example 2: Western Corn Rootworm (WCRW) Adult Sterilization by VgR dsRNA
[0255] Artificial diet for WCRW adults was prepared using a modified protocol (Rangasamy M et al. (2012). Pest Manag. Sci. 68(4):587-91; and Nowatzki T M, et al. (2006) J Econ. Entomol. 99(3):927-30). The modified diet was designed for use in the diet incorporated bioassay described herein below (25 .mu.l test sample:75 .mu.l prepared diet) and was produced using standard 96-well micro-titer plates. WCRW adults consumed significant proportions of the diet within 24 hours, and control mortality remains <15% during the 2-3 week study period. For all the bioassays described herein, WCRW life stages (adults and larvae) were kept under standard conditions, for example, Jackson, J. J. (1986), pp 25-48. In Krysan J. L. and Miller T. A. (eds): Methods for the Study of pest Diabrotica. Springer-Verlag, New York. 260 pp, and Branson, T. F., et al. (1988), J. Econ. Entomal. 81(1): 410-414 (1988).
[0256] Beetles from the same batch were categorized in to two groups. The first test group consisted of male and females of <10 days old (range 2-5 days old) (hereinafter referred to as "younger females"). The younger females were in their preoviposition period (the period before oviposition of the first eggs). The second group consisted of >11 days old and mated females (hereinafter referred to as "older females") and they were in oviposition period (females are ready to lay eggs). For the younger female group 100 beetles (50 females and 50 males) and for the older female group 50 mated females were arranged for each treatment. The following three treatments were compared 1) sterile DI water (control); 2) GFP dsRNA (negative control, GenBank Accession # AY233272.1; SEQ ID NO: 104 herein); and 3) VgR dsRNA fragment 2 (SEQ ID NO: 4). The bioassay was carried out using a diet incorporation methodology. Test samples of GFP dsRNA and VgR dsRNA were prepared separately and 25 .mu.l of the respective samples were incorporated into 75 .mu.l of modified WCRW adult artificial diet per well in 96-well micro-titer plates for a final concentration of 100 ppm. For control 25 .mu.l of sterile DI water was incorporated into 75 .mu.l of modified WCRW adult artificial diet per well.
[0257] The effects on individual WCRW adult beetle were confined using individual wells of 32 cell trays (C-D International, Pitman, N.J.) provided with single diet pill (mixture of 25 .mu.l test sample and 75 .mu.l of modified WCRW adult artificial diet) for 24 hours. After 24 hour, treated adults were collected, counted and transferred to their respective holding cages and provided standard SCRW dry adult diet and water source until the end of the study period (22-25 days).
[0258] WCRW eggs were collected daily for 13-14 days starting from 24 hour or 7 days after exposure for the older and younger female group, respectively. Eggs were collected using oviposition dish. Collected eggs were incubated in a heat and humidity controlled growth chamber (25.degree. C., 65%.+-.5% relative humidity (RH)) with controlled light/dark cycles (16 hr light:8 hr darkness) for 12-14 days before processing.
[0259] Several small aliquots of egg-agar suspensions were dispensed onto a hatch plate (petri-dish containing 2% water agar and two layers of filter paper) for counting and/or hatch test depending on the number of eggs obtained for a given day. For the egg hatch test, samples (1-6) each containing 25 .mu.l of egg-agar suspension were dispensed onto the hatch plate as described above and the lids secured with micropore tape to avoid larval escape. Total number of eggs in each 25 .mu.l sample was counted prior incubation. Egg hatch plates were then incubated in a heat and humidity controlled growth chamber (25.degree. C., 65%.+-.5% RH) with controlled light/dark cycles (16 hr light:8 hr darkness) for three days. Egg hatch was counted over three days period by counting the number of eggs showing larval emergence hole. For each treatment, four treated female and male beetles (younger female group) and four females (older female group) were sampled for gene suppression at 4 and 8 days after exposure for the older and younger female group respectively.
[0260] For dsRNA in vitro transcript ("IVT") production, PCR was performed using target specific forward and reverse primers (see Table 2 below) with a T7 promoter sequence at the 5' end of each primer. The dsRNA samples were produced from PCR template using Ambion Megascript High Yield Transcription Kit (Thermo Fisher Scientific, Grand Island, N.Y.). An agarose gel was run to check for yield and product size. For real time qRTPCR assay, total RNA was extracted with MirVana miRNA Isolation Kit, treated by TURBO DNase Kit, assayed by SuperScript.RTM. III Platinum.RTM. One-Step qRT-PCR Kit with ROX according to manufacturer's instructions (Thermo Fisher Scientific). Relative expression was derived by delta delta Ct method (Livak, K. J. and T. D. Schmittgen (2001). Methods 25(4): 402-408) using WCRW RPS10 as reference (i.e., SEQ ID NO: 8 in US 2011/0054007; also SEQ ID NOs.: 102 and 103, ORF and transcript, respectively, herein).
TABLE-US-00002 TABLE 2 Primer Sequences IVT Production. Forward Primer Reverse Primer Fragment ID SEQ ID NO. SEQ ID NO. DV-VGR-FRAG1 54 55 DV-VGR-FRAG2 56 57 DV-VGR-FRAG3 58 59 DV-VGR-FRAG4 60 61 DV-VGR-FRAG5 62 63 DV-VGR-FRAG6 64 65 DV-VGR-FRAG7 66 67 DV-VGR-FRAG8 68 69 DV-VGR-FRAG9 70 71 DV-VGR-FRAG10 72 73 DV-VGR-FRAG11 74 75 DV-VGR-FRAG12 76 77 DV-VGR-FRAG13 78 79 DV-VGR-FRAG14 80 81 DV-VGR-FRAG15 82 83 DV-VGR-FRAG16 84 85 GFP 86 87 GUS 88 89
[0261] Data obtained using these methods are shown in FIGS. 1A-1D. In particular, FIG. 1A shows the total number of eggs produced within 13-14 days by treatment and age group. In the experiment shown in FIG. 1A, the younger female group contained 50 pairs of male and female beetles, whereas the older female group had 50 mated female beetles. The data in FIG. 1A show that ingestion of the VgR dsRNA significantly reduced the total number of eggs produced during the test period. FIG. 1B shows the average number of eggs produced per female/day during 13-14 day oviposition period by treatment and age group. The box plot shows 4 quartiles, average, and 95% confidence interval of the mean. The data show that in both younger and older females, ingestion of the VgR dsRNA reduced the average number of eggs produced per day. FIG. 1C shows the effect of various treatments on overall average egg hatch rate. Data represents 13-14 days egg collection period; n=6 replication/treatment/day; 5-45 eggs/replication depending on the day (p<0.001). Gene suppression analysis is shown in FIG. 1D for analysis carried out on WCRW adult beetles 8 days after treatment of female and male insects for younger age group and 4 days after treatment of female insects for older age group. Relative expression of VgR is shown from 4 individual insects for each treatment using WCRW RPS10 gene as reference and untreated older beetle as normalizer. The box plot shows 4 quartiles, average, median, and 95% confidence interval of the mean by treatment and age group.
Example 3: WCRW Sterilization by Treatment of 3rd Instar Larvae with VgR dsRNA
[0262] The effect of treatment of larva on WCRW sterilization by VgR dsRNA (VgR dsRNA fragment 2) was assessed. The study was carried out using 3rd instar larvae that were harvested from corn mats and acclimatized on standard WCRW larval diet for 24 h. About 192 larvae were exposed to water and 75 ppm VgR dsRNA fragment 2 (SEQ ID NO: 4) for 1 day using the diet incorporation method described above (25 .mu.l dsRNA and 75 .mu.l artificial WCRW larval diet). Treated larvae were placed in pupation medium for 15 days. Emerged adults were collected, counted, and transferred to their respective holding cages and provided standard SCRW dry adult diet with a water source until the end of the study period (22-25 days). Beetle holding cages were kept at room temperature (usually from 22-25.degree. C.) with no RH control. No intentional light/dark control but cages were getting roughly 16:8 Dark and light condition. Beetle holding cages were cleaned maintained twice a week and each time the beetles received new food and water agar. Adult beetles were kept for a total of 26 days. Each cage received oviposition dishes after 10 days preoviposition period and eggs were collected over a period of for 16 days oviposition period, and processed following the method described above.
[0263] Representative data for this study are shown in FIGS. 2A and 2B. The average total number of eggs produced per female and the average number of viable eggs produced per female are shown in FIG. 2A. Eggs from 15-42 female adult beetles were counted for each indicated treatment. The box plot of shows 4 quartiles, average, median, and 95% confidence interval of the mean for each treatment. The data show that for the VgR dsRNA exposed group, the viable egg production remained very low throughout the study period. It should be noted that treatment with VgR dsRNA did not affect adult emergence, and that mortality of adult beetles in the VgR dsRNA group was negligible.
[0264] Representative data for VgR gene suppression analysis is shown in FIG. 2B. The data were obtained for 10-day old (n=4) and 28-day old (n=15) beetles, which represents day 40 and day 58, respectively, following treatment of the 3.sup.rd instar larvae. The box plot of relative expression by qRTPCR shows 4 quartiles, average, median, and 95% confidence interval of the mean for each treatment in 10 and 28 day old beetles. The data were normalized to untreated 3rd instar larvae. The data show decreasesd relative expression of VgR in both age groups.
Example 4: Dose Response of WCRW Sterilization and Gene Suppression by VgR dsRNA Treatment
[0265] The dose response effect of dsRNA treatment was determined in younger and older adult females. The older female group (>11 days old) was collected and exposed VgR dsRNA using the diet incorporation methodology described above. The treatment groups were exposed for 24 hours. The VgR dsRNA was complementary to SEQ ID NO: 3, and the concentrations tested were as follows: 0, 0.01 ppm, 0.1 ppm, 1 ppm, 10 ppm and 75 ppm. The treatment groups consisted of about 40-48 females for each dose level. Egg production was assessed starting 24 hours after exposure and continued for 18 days. For each treatment, the total number of female beetles used for egg production varies from 40-48 (days 1-6) and 20-28 (days 7-18). Eggs were handled and processed following the methods described above. Six day after exposure 20 treated females were retrieved from each treatment and were used for gene suppression analysis.
[0266] The details for each dose treatment group (e.g. number of total eggs analyzed, number of viable eggs, and net reduction in fecundity) are given in FIG. 3A. Eggs were collected and counted over the 18 day oviposition period. The net reduction in fecundity (NRF) of VgR dsRNA-treated females relative to control (water exposed females) was estimated using the following formula:
NRF (%)=[1-(NVEt/NVEwc)]*100,
where "NRF" represents the net reduction in fecundity as a percent; "NVEt" represents the number of viable eggs in the treatment group; and "NVEwc" represents the number of viable eggs in water (control) treated group. The data show a significant reduction in egg production after 10 days of exposure to the VgR dsRNA (see eggs/female day 10-18 in FIG. 3A). The data further show that egg production and viability of eggs were negatively correlated with VgR dsRNA doses. The net reduction in fecundity was positively correlated with increased vgR dsRNA doses. FIG. 3B shows a box plot of percentage of overall egg hatch rates by dose for the 18 day egg collection period; n=1-4 replication/treatment/day; 5-478 eggs/replication depending on the day and availability of eggs. The data in FIG. 3C show a box plot of relative expression of VgR at day 6 after VgR dsRNA treatment at different doses. The data in FIG. 3C show the correlation of increasing dose with a larger decrease in expression of VgR. The treatment group data were normalized to the expression for untreated beetles.
Example 5: Gene Suppression Analysis of VgR Fragments
[0267] The effect of five distinct VgR dsRNA target fragments was assessed. FIG. 4A shows schematically the relative position of the different fragments tested aligned against SEQ ID NO: 2. The target fragments tested were as follows: Frag1 is VgR fragment 1 (SEQ ID NO: 3); Frag2 is VgR fragment 2 (SEQ ID NO: 4); Frag3 is VgR fragment 3 (SEQ ID NO: 5); Frag4 is VgR fragment 4 (SEQ ID NO: 6); and Frag5 is VgR fragment 5 (SEQ ID NO: 7). Each VgR dsRNA fragment was tested using the diet incorporation methodology described above with WCRW female beetles with the VgR dsRNA at 100 ppm in the diet plug. The beetles were treated individually for one day and fed with standard diet with no dsRNA for an additional six days. The individual beetles were then collected and flash frozen in liquid nitrogen. For the qRTPCR assays, at least 3 insects were used for each treatment group using the primer sequences indicated in Table 3 below. FIG. 4B shows a box plot of the relative VgR expression at day 6 after treatment with the indicated dsVgR fragments or control treatment (i.e., ddH2O and dsGUS (SEQ ID NO: 105, herein), as indicated, replacing the VgR dsRNA in the diet) using 5' qRTPCR assay. The box plot shows four quartiles: average (horizontal solid line), median (horizontal dash line), and 95% confidence interval of the mean are shown. Similar results were also obtained with Mid- and 3'-qRTPCR assays. The data in the treatment groups were normalized to data obtained from qRTPCR from untreated 3rd instar larvae.
TABLE-US-00003 TABLE 3 Primer Sequences Gene Suppression Analysis. Forward Reverse Amplicon qRTPCR Primer Primer Amplicon Length Assay ID SEQ ID NO. SEQ ID NO. SEQ ID NO. (nc) DV-VgR 5' 90 91 92 147 DV-VgR mid 93 94 95 140 DV-VgR 3' 96 97 98 78 DV-RPS10 99 100 101 77
Example 6: WCRW VgR Fragment Screen by Gene Suppression Analysis
[0268] Additional VgR dsRNA fragments covering the entirety of the coding DNA sequence of SEQ ID NO: 2 were assessed for ability to suppress expression of VgR. The fragments tested are shown aligned against SEQ ID NO: 2 in FIG. 5A, and the various sequence names correspond to the fragment ID shown in Table 1. Each VgR dsRNA fragment was tested using the diet incorporation methodology described above with WCRW female beetles with the VgR dsRNA at 100 ppm in the diet plug. The beetles were treated individually for one day and fed with standard diet with no dsRNA for 6 additional days. The individual beetles were then collected and flash frozen in liquid nitrogen. For the qRTPCR assays, at least 6 insects were used for each treatment group. FIGS. 5B-5D box plots of the relative VgR expression at day 6 after treatment with the indicated dsVgR fragments or control treatment (i.e., ddH2O and dsGUS, as indicated, replacing the VgR dsRNA in the diet) using 5' qRTPCR assay. The data in the treatment groups were normalized to data obtained from qRTPCR from water treated beetles. Two qRTPCR assays (5'- and Mid-qRTPCR assays) were used to avoid overlapping of VgR fragment and PCR amplicon.
Example 7: Agrobacterium-Mediated Transformation of Maize
[0269] For Agrobacterium-mediated transformation of maize with a silencing element of the invention, the method of Zhao is employed (U.S. Pat. No. 5,981,840, and PCT patent publication WO98/32326; the contents of which are hereby incorporated by reference). Such as a construct can, for example, express a long double stranded RNA of the target sequence set forth in table 1. Such a construct can be linked to a promoter. Briefly, immature embryos are isolated from maize and the embryos contacted with a suspension of Agrobacterium, where the bacteria are capable of transferring the polynucleotide comprising the silencing element to at least one cell of at least one of the immature embryos (step 1: the infection step). In this step the immature embryos are immersed in an Agrobacterium suspension for the initiation of inoculation. The embryos are co-cultured for a time with the Agrobacterium (step 2: the co-cultivation step). The immature embryos are cultured on solid medium following the infection step. Following this co-cultivation period an optional "resting" step is contemplated. In this resting step, the embryos are incubated in the presence of at least one antibiotic known to inhibit the growth of Agrobacterium without the addition of a selective agent for plant transformants (step 3: resting step). The immature embryos are cultured on solid medium with antibiotic, but without a selecting agent, for elimination of Agrobacterium and for a resting phase for the infected cells. Next, inoculated embryos are cultured on medium containing a selective agent and growing transformed callus is recovered (step 4: the selection step). The immature embryos are cultured on solid medium with a selective agent resulting in the selective growth of transformed cells. The callus is then regenerated into plants (step 5: the regeneration step), and calli grown on selective medium are cultured on solid medium to regenerate the plants.
Example 8: WCRW VgR Transgenic Feeding Bioassay
[0270] Transgenic maize plants for VgR Frag1, Frag2 and Frag3 were generated using the methods described herein above and used in adult feeding bioassays for gene suppression analysis in WCRW beetles as described herein above. The expression levels of the VgR fragments in planta were determined in leaf samples using in vitro transcription (IVT) products as controls. The expression analyses were carried out according to manufacturer's instruction (Quantigene 2.0 Assay, Affymetrix, Santa Clara, Calif. 95051). The average VgR fragment expression level (pg VgR fragment/mg fresh plant weight) in leaves is indicated at below each graph in FIGS. 6A and 6B. FIG. 6A shows data obtained from three young plants at about the V4 growth stage for either a non-transgenic control (NTG) or the indicated transgenic planted expressing the indicated fragment VgR Frag1 (SEQ ID NO: 3), Frag2 (SEQ ID NO: 4), and Frag3 (SEQ ID NO: 5). The test plants were infested with at least 14 young female beetles in cages. The beetles were collected at 8 days after feeding and used for gene suppression analysis. Data were normalized to expression levels obtained in beetles exposed NTG plants. FIG. 6B shows results obtained using either non-transgenic control plants or transgenic plants expressing the indicated VgR dsRNA fragment. The individual R1 maize plants were infested with at least 6 young female beetles in cages. Beetles were collected 12 days after feeding for VgR expression analysis. Each fragment and control is represented by 2 plants used for feeding and more than 12 insects used in gene suppression analysis, and data were normalized to data obtained from non-transgenic control plants exposed under similar conditions.
Example 9: WCRW Adult VgR Transgenic Exposure Bioassay
[0271] At least 32 pairs of newly emerged adult beetles were exposed to 8 days to above ground plant part of T1 transgenic events or non-transgenic (NTG) control plants. Beetles were recollected and at least 13-37 female beetles were arranged in a cage for each treatment and maintained for 15 days for fecundity assessment. Transgenic constructs expressed VgR Frag1 (SEQ ID NO: 3), Frag2 (SEQ ID NO: 4), or Frag3 (SEQ ID NO: 5). For each construct 2-4 events were tested (FIG. 7). Each cage received oviposition dish daily and/or at interval of 2-4 days and eggs were processed following the method described in Example 2.
Example 10: WCRW Larval VgR Transgenic Exposure Bioassay
[0272] Maize T1 plants expressing silencing elements (targeting SEQ ID NOs: 3, 4, and 5) were transplanted from culture plates into greenhouse flats containing Fafard Superfine potting mix. Three positive individual plants (of same event) were transplanted and maintained in a greenhouse (80.degree. F., 15 hr light:9 hr darkness) and watered as needed. When the plants reached the V2 leaf stage, each pot was infested with 200 non-diapausing D. virgifera virgifera eggs. Plants were monitored daily for first beetle emergence. The number of adult D. virgifera virgifera that emerged from each pot was determined in the greenhouse in a similar manner as described by Meihls et al. (2008) PNAS 105: 19177-19182. Adult beetles emerged from T1 transgenic events and non-transgenic (NTG) control plants as in Example 9 were collected every 2 or 3 days and maintained for 15 days for fecundity assessment. For each event three replicate cages containing at least 8-14 pairs of male and female beetles were arranged. Each cage received oviposition dish every 5 days and eggs were processed following the method described in Example 2 (FIG. 8).
Example 11: WCRW Adult Exposed Sterilization Bioassay and Gene Suppression by Treatment of dsRNA Targeting BOULE
[0273] Virgin adult beetles were obtained by rearing 3.sup.rd instar larvae individually in a 50 mL falcon tube containing the pupation medium. Beetles were sexed upon emergence; starved for 24 hours and exposed at 100 ppm of dsRNA targeting the BOULE target gene (DV-BOULE-FRAG1, SEQ ID NO: 164) or controls (sterile water or GUS dsRNA) using diet incorporated method for one day. Treated beetles were provided untreated diet and kept in solitary confinement for additional 5 days. At least 12 treated beetles of mixed sex were collected in liquid nitrogen for gene suppression analysis. At least 14 pairs (male and female) were arranged for each treatment for subsequent mating and fecundity assessment. Due to delay in mating, oviposition was begun 19 days after emergence and egg production was assessed for 10 days. As shown in FIG. 9A, high dose adult exposure to dsRNA targeting BOULE (BOULE dsRNA, SEQ ID NO: 164) did not affect egg production and hatch rate of the eggs. FIG. 9B shows gene expression in beetles after BOULE dsRNA (SEQ ID NO: 164) treatment. Relative expression by qRTPCR assay was performed as in previous examples.
Example 12: WCRW Beetle Counts from Larval Exposure to T1 Transgenic Plants Expressing dsRNA Targeting BOULE
[0274] Three maize seedlings of V2 leaf stage T1 transgenic events expressing dsRNA targeting BOULE using DV-BOULE-FRAG1 (SEQ ID NO: 164) and non-transgenic (NTG) control plants were infested with 200 WCR eggs per pot in greenhouse. Approximately one month after infestation, beetles emerged from each pot were captured every 2 or 3 days over 10 days. Average beetle numbers for the total and each sex (Male and Female) from at least 9 pots per event and 10 NTG control pots are shown. The box plot shows four quartiles, average (horizontal dash line), median (horizontal solid line), and 95% confidence interval of the mean. The results suggest that exposure of WCRW larvae to transgenic events expressing DV-BOULE FRAG1 (SEQ ID NO: 164) dsRNA did not affect adult emergence pattern when compared to NTG plants (FIG. 10). Average expression levels of the BOULE fragment in planta for each event were determined in root samples using in vitro transcription (IVT) product as control as described in Example 8.
Example 13: WCRW Larval Exposure to Transgenic T1 Plants Expressing dsRNA Targeting BOULE Caused Adult Sterilization
[0275] Beetles emerged from T1 transgenic events expressing DV-BOULE-FRAG1 (SEQ ID NO: 164) dsRNA and non-transgenic (NTG) control plants were maintained for 25 days for fecundity assessment. For each event three replicate cages containing at least 8-14 pairs of male and female beetles were arranged. Each cage received oviposition dish every 5 days and eggs were processed following the method described in Example 2 except that egg hatch duration was extended up to 8 days. FIG. 11A shows the effect of larval exposure to transgenic plants expressing DV-BOULE-FRAG1 (SEQ ID NO: 164) dsRNA on the overall average egg production per female and average viable eggs produced per female from emerged beetles. FIG. 11B shows the effect of larval exposure to transgenic plants expressing DV-BOULE-FRAG1 (SEQ ID NO: 164) dsRNA on hatch rate of eggs obtained from the emerged beetles. FIG. 11C indicates the effect of larval exposure to transgenic plants expressing DV-BOULE-FRAG1 (SEQ ID NO: 164) dsRNA on net reduction in fecundity of emerged adult beetles relative to NTG control.
Example 14: WCRW 3.sup.rd Instar Sterilization Bioassay of Exposure to dsRNA Targeting MAEL, NCLB and CUL3 at 1 ppm
[0276] Over 400 3rd instar larvae were exposed to 1 ppm of the respective dsRNA samples (DV-MAEL-FRAG1, SEQ ID NO: 46; DV-NCLB-FRAG1, SEQ ID NO: 45; and DV-CUL3-FRAG1, SEQ ID NO: 44) following the method described on Example 3. Water and GUS treatment were included as experimental controls. Treated larvae were separated into four groups, each containing about 95 to 100 treated larvae and placed in pupation medium for 15 days. Emerged adults were collected, counted, and transferred to their respective holding cages and handled as described in Example 3. After 10 days of oviposition period the number beetles in each replicate cage was adjusted to at least 12-16 pairs (male and female) and fecundity was assessed for 15 days. At least 2-3 replicate cages were setup for each treatment. The average total number of eggs produced per female, the average number of viable eggs produced per female, the average egg hatch rate; average reduction in egg production and net reduction in fecundity (both relative to water control) are shown in FIG. 12.
Example 15: WCRW Sterile Gene Screening by 3.sup.rd Instar Sterilization Bioassay at 50 ppm
[0277] The effect of treatment of larvae on WCRW sterilization by dsRNA targeting various genes of interest (GOI) was assessed using 3.sup.rd instar. A set of 8-14 dsRNA samples including water and GUS control was tested at a time. The study was carried out using 10 day old 3rd instar larvae that were harvested from corn mats and acclimatized on standard WCRW larval diet for 24 hours. Diet incorporation method was used for exposing larvae to 50 ppm final concentration of the respective test dsRNA samples in 2 mL per well of 6-well plate. A total of three 6-well plates were prepared for each sample and about 312 larvae were exposed to water or 50 ppm of target dsRNA fragment (GOI) for 1 day (.about.104 larvae/plate; 16-18 larvae/well). After exposure, .about.10 3.sup.rd instar larvae were sampled for gene suppression analysis and the remaining treated larvae were placed in pupation medium for 15 days. Emerged adults were collected, counted, and transferred to their respective holding cages and provided standard SCRW dry adult diet with a water source (water agar) until the end of the study period (.about.26 days). Beetle holding cages were kept at room temperature (usually from 22-25.degree. C.); 16:8 dark and light condition with no relative humidity control. After 10 days of preoviposition period, for each treatment the number of male and female pairs was adjusted to 16-20 pairs depending on beetle density and each cage received oviposition dishes and eggs were collected over a period of for 15 days oviposition period, and processed following the method described in Example 2. A set of three males and three females were sampled 10 and 25 days after emergence for gene suppression. Table 4 shows a consolidated summary of egg production, egg hatch and reduction in egg production and fecundity for active WCRW gene targets. Column 1 indicates GOI (gene of interest), column 2 and 3 indicate total egg production/female and total viable eggs/female respectively during the 15 days egg production period; column 4-5 indicate cumulative average egg hatch (.+-.SEM); column 6 and 7 indicates average reduction in egg production (%) (.+-.SEM); column 8 and 9 indicate average net reduction in fecundity (%) (.+-.SEM). Note that values for controls (water and GUS) are cumulative average of 11 independent experiments, each run for the duration of 15 days of egg production period.
TABLE-US-00004 TABLE 4 WCRW sterile gene screening by 3rd instar sterilization bioassay at 50 ppm* Total No. Avg. Egg Reduction in egg Net reduction Total No. fertile Eggs/ hatch (%) .+-. production (%) .+-. in fecundity (%) .+-. SEQ ID Target Gene Eggs/female female (SEM) (SEM) (SEM) NO: MEI 0 0 0.0 0.0 100.0 0.0 100.0 0.0 133 KNRL 96 0 0.5 0.3 59.6 9.4 99.6 0.1 132 TUD 7 1 15.0 0.8 95.5 0.8 98.6 0.2 118 CG3565 172 2 1.4 0.4 28.0 10.7 98.0 0.3 130 CG17083 160 3 1.7 0.6 32.8 20.5 97.7 0.7 129 DM 96 8 8.7 2.7 73.5 6.0 95.7 1.0 125 CYCA 26 8 29.9 7.6 89.5 2.1 93.6 1.3 43 HIRA 63 5 7.6 1.8 57.5 6.5 93.1 1.1 116 Poe 41 8 16.8 2.9 74.2 7.2 91.9 2.2 113 EGG 52 20 38.7 2.9 85.8 4.7 89.8 3.4 126 MR 45 21 45.9 3.5 87.6 2.2 89.4 1.8 128 HANG 129 22 17.3 2.6 54.0 6.2 84.3 2.1 108 HTS 125 14 10.8 2.5 23.5 13.0 81.9 3.1 122 GSKT 82 26 32.3 3.4 67.7 4.8 80.6 2.9 40 ADE2 70 28 40.3 8.3 75.1 7.0 80.1 5.6 107 DLG1 202 39 19.3 4.8 44.3 7.1 79.9 2.6 124 SU(VAR)205 132 32 24.3 4.7 52.7 9.3 77.3 4.4 111 CDK7 148 45 30.1 3.6 59.2 7.0 77.1 3.9 123 HRG 200 50 25.2 4.5 45.0 9.4 74.2 4.4 127 MBD-like 85 17 20.4 3.4 41.2 11.9 74.0 5.3 114 11NUP44A 112 29 26.1 2.8 46.2 12.5 70.4 6.9 120 CASP 150 27 18.0 4.8 13.4 12.4 70.2 4.3 42 FAF 101 30 29.8 3.5 51.5 7.9 69.5 5.0 119 TWE 257 38 14.9 1.7 -7.6 10.3 67.4 3.1 134 PORIN 105 46 44.2 4.8 62.5 4.6 67.1 4.0 110 PARK 64 31 46.3 4.0 60.1 6.4 66.8 5.4 112 PGLYM78 80 22 27.8 4.4 44.5 13.5 66.5 8.2 115 WTS 103 46 44.7 3.6 59.5 5.9 66.4 4.9 41 PUF 89 25 28.3 3.9 40.3 8.3 64.2 5.0 117 KL3 130 55 42.4 7.8 53.4 8.7 60.8 7.3 109 GUDU 211 55 26.2 3.0 16.9 6.2 59.6 3.0 39 CYCB 214 49 22.7 2.6 10.2 6.9 58.6 3.2 131 GEK 136 36 26.2 2.3 17.4 9.9 52.6 5.7 121 REPH 183 72 39.2 4.0 27.7 12.5 47.3 9.1 165 ARMI 316 108 34.2 4.4 12.9 10.9 44.5 7.0 166 loqs 148 42 28.4 3.9 9.8 15.8 44.1 9.8 167 SCNY 165 72 43.5 3.2 31.0 5.1 39.0 4.5 168 AGO3 186 46 24.9 2.5 -13.7 14.0 38.1 7.6 169 DIA 288 124 43.0 4.9 20.8 9.9 36.5 7.9 170 DNC 274 101 37.0 3.3 9.5 21.5 36.1 15.2 171 Chi 269 103 38.2 3.8 11.3 18.3 35.3 13.3 172 SXL 188 66 35.2 3.6 10.2 13.7 33.3 10.2 173 SLGA 199 94 47.1 3.9 21.5 8.1 31.3 7.1 174 PAPLA1 150 53 35.4 2.7 8.7 11.6 29.4 8.9 175 GUS* 187 88 43.8 1.3 12.1 4.3 16.8 5.1 H20* 217 110 50.0 1.0 *Column 1 indicates GOI (gene of interest), column 2 and 3 indicate total egg production/female and total viable eggs/female respectively during the 15 days egg production period; column 4-5 indicate cumulative average egg hatch (.+-. SEM); column 6 and 7 indicates average reduction in egg production (%) (.+-. SEM); column 8 and 9 indicate average net reduction in fecundity (%) (.+-. SEM). Note that values for controls (water and GUS) are cumulative average of 11 independent experiments, each run for the duration of 15 days of egg production period.
Sequence CWU 1
1
25415612DNADiabrotica virgifera 1tggtcagttc gtcctttaaa actgtgcgtg
ttaaactttt tgaatatctt gtaaagtatg 60ttaattttgt tctttgtgct ttcaacgtta
ttaaatgcag tcgtttcaac gaataataat 120gattgccctc cacatcagtt
tagatgcaca aattcaaaat gtatagactt tcaacaaaga 180tgtgatggtt
ccgacaattg tggagatgga tcggatgaac tggattgtga tcataaatgt
240catgaacatt ttttcaattg tcgaaatgga agatgtataa gcgaagcatt
cctgtgtgat 300ggtgaaaatg attgtaataa ctttttggat gaaaaagact
gtaagggtca aacggtgcta 360actctcgaaa ctgatggcca ttgcgaagat
ggtcattgga agtgtgccga taaattgtgt 420attccacttg acatggtatg
taatggcgca cctgaatgtt tggatggatc agatgaaact 480attggatgca
gtaacaagat cgtttgtgat ggatttaaat gtaaaaatgg ccattgcata
540ccaactgaat gggcttgcga cggtcttgat gattgtggtg ataaaactga
tgaacaagat 600tgcgcaaacc acgtaccact tgatcaatgc actttggata
aaagaaaatt cttgtgtagt 660aacaataaaa cttgcataga tcttcactcg
gtctgtgata ataagttaga ttgtcccgat 720tattctgatg aaggaaaaca
gtgcaattcg tctgcaatat catgtaacaa taacaaatgt 780tctcatacat
gtatatcact tccaacgggt ccaaaatgtc tgtgtccgaa tggttaccat
840acgcttgatg attcgaattg tttggatatt aatgaatgta caacttatgg
aatttgtgac 900caaaaatgta gaaatcttcc tggatcatac gaatgctact
gtgatgataa gtacacactc 960caagccgata aaaaaacatg taaagcaact
ggcggaagcg gaataatgat ctttagttcc 1020aagcaggaaa ttcgagcttt
aaccttagat tctttagatt attttacagt ggctaaagat 1080ttaaatcaag
tagtgggaat agcatacgat ggacatcata tttactggac agacgtattt
1140actggacatg aaactatatc gaggtctatt gaagatggat cggaaaggga
ggtattggta 1200acatctggac tcagtctacc agaggatcta gcgtatgatt
ggttaactgg taatatttat 1260ttcaccgatg ctctcaaaca acatgttgga
gtgtgttcca atgatggtca ccattgtaca 1320gttctagtca acaaagatat
tcgaaaacca cgtgggattg ttgttaatgt ggaagctgga 1380gatatgtatt
ggtctgattg gggtgtacca gcagcaattg gatattcact tatggatgga
1440tcccaggaca aaccgttcgt caccaataat atacactggc ccaatggttt
ggctctagat 1500caacctaact ctagacttta ttggactgat gctaaaaaga
tgacactaga aagtattaat 1560ttagacggaa ctgatcaaag gatcgttttg
gaaggaatag tgaaacatcc atatgccatc 1620gctgtgtttg aaaacaaact
ttattggtca gattgggact cccataccat tcaaacttgt 1680gataagttta
atggtaaaaa tcatcataca ctcgtggaag aacataaaaa tttaatctat
1740ggaatcagta tttttcacta tgctctcgaa aagcgtctag tgaatccttg
tgagcatgct 1800tcctgtagtg acatttgttt attgaaggct cagtcatatg
cttgtgcgtg cccagagaat 1860aaagttttgg atacggatgg tcatacgtgc
aaagaagtac aaccaacgca agcacttgta 1920ggaggaactg gacacgtgtt
ggtatcaatt aaacatcaat ttctaggcaa acacgatgta 1980acattcctac
cagacctggc gaaacacgta ggaagcttag catttgatag ttataaaaac
2040attttgtatg taagcgattt ggaaacaaaa agtatagttg cgcttaacat
gaacatcgga 2100attagcaaaa ctcttgagat cgacggtctt ggcaaagtta
cttcaatgga ttttgatcca 2160aaaagtaata atttatacat ttgcgatacg
gagaggctag tcgtagaaat aattgatatg 2220aataatctag aaagaaaaat
aattgtccat gacacatttg gagaaactcc tgaaagcatt 2280gcgttggtac
cggaagatgg gatcatgttt gttagcttta aacaagggaa attgggaaat
2340agccacatcg accgtttttt catggatggt actggaagaa ctcacgtcat
cgaaaacggt 2400ttagtgggcc cagttcgtgt agtatacgac cgcagtcttt
atagaatttt ctttgccgat 2460ctaagtactg gggtaataga atctactagc
gcggccggtg acgatagaca tcattttcgt 2520actctgacta cccatccagt
aagtatagca gttttgaaag atgacctatt gtggacaaat 2580cttgactcca
aggatttata ttggtctgaa aaaagatcta gcgagtctta tgagaagaaa
2640attacgatag gttttaaaga ggataacgtc aacatccact tagtgtcagt
aacacgtaaa 2700caattagaaa tcaatagctg tcgtgttaat aataatggat
gtagtcatct ttgtcttcaa 2760tcccataaat cgatagtttg tgcatgtcca
gctggatggg agctatcggc aaatggtttt 2820acatgtaata aaagggtaac
ctgcgacaat aaagagatgc tttgtcctca ttccaatact 2880tgcgttctga
agagcttaag gtgcaatggt tttaaagact gcgcatttgg tgaagatgaa
2940tgggactgcc aaactgtttc ccaatgttta cctggccagt ataaatgcga
cgatggacag 3000tgcatcagtg aagatttggt ctgcaatcat agttatgact
gtaaagataa gtctgatgag 3060tatggatgtg cagataaaaa caaaaaacta
ggatgtcctc ccggacattt tacctgtaaa 3120agctccgaat gcatatctga
acgctttgta tgtgatgctt tccatgattg cgatgatgga 3180tcagatgaac
taaactgcga aaataatatt tgtttggaat ctcagtttag gtgtgatgtc
3240ggaacttgca taccaaaaga ttgggaatgt gatggagaat atgactgtac
tgatagttca 3300gatgagcatt gttcttcaga agtttgtcag tctaactatt
ttaagtgtga caacaacaga 3360tgtatcgatc ctaagcttca gtgcgatggg
tttgatgact gcggtgatca ctctgatgaa 3420aaattcgaga aatgtttgca
tcactcgaaa gcaccaaaat gtacattgga agaattcgcg 3480tgtattacta
atacttccat ttgtcttcca aaatcagcta aatgtaacgg aacgtcagaa
3540tgtccgaata acgaagacga aaaggattgt tcaaaatgtg aagaagatga
atttgagtgc 3600aagcataagc attatagaga atgtattcct aggtcctgga
tatgtgatgg tacagatgat 3660tgcggtgata atagtgatga gagtatggaa
acctgttctt caaaacttaa acctgcggca 3720gataactttg ctgttactga
ctcgtgtcta accggatata gatgtcatag cggtgcatgc 3780ataaatatga
cctcagtatg caataataac cacgattgtt ttgatggctc tgatgaagat
3840ggcctctgtt cttcatcttg tgtcggtgtg aaaaatcctt gcaatcaaat
atgcgtaaaa 3900acaccatctg gaccaagatg tgaatgtaaa cctggatata
agttgttagg agatggaaaa 3960acttgtattg atgataatga atgccaaacg
gatccaccaa tctgtagtca gctatgtcac 4020aataaagaag gaggctattc
ttgcgattgt tacaataatt tccttttaag ttctaataaa 4080aaatcttgta
aagctcacgg accaagaatg accatatact tagtgattta cggaaaccaa
4140atccgtcacc taattcctaa atcaaacaca atggctgttc tttacaccaa
tccaatgata 4200aaaatatcca gcttggatac tctggttaaa ccaaaactaa
tcttcttcag ttcttatgaa 4260actcaagcta tttataaact tgataccacc
accaacatga tgcactacat cagaaacgta 4320ggtttcccta gaacaattgc
agttgattgg tcaactcaaa acatttatta cttcgatact 4380gatgttaatg
gccggtccat tagtgtttgc agttttgaag aaaagtgcgc taagttgata
4440aatattgaat cgcctcgaca tgtaactgcc ttagctgttg attccgtcaa
taaattgctt 4500ttctacgttt tgaggaattg gtgggtatta gaaacaccaa
gcttcgtaat atacagtgta 4560aacttggatg gatctaatag acaagaaata
gtaaaaacaa caacgggcaa tgttgaagat 4620ataactttcg atattaacaa
gaaactttta tactatacta acaaacaaga tgaaagtata 4680cacgaagtaa
gctacaaagg aggaccggtt aaaactgtat tttcaaatat ctctcaacca
4740gaaggactta aattttttga gaaccaatta tactatactg ttgcaactgg
ttatatagtc 4800agttgtaagc tatatggaga cagatcctgc aatcccagat
ataaattaca cacgtttgcc 4860agtggtcaat ttgttattga gcaggaatcg
cttcagccta aagtagaaaa tgtttgtact 4920ggacacaaat gtccatactt
atgtgtacca gcagaatcag gatatagatg tctctgtcat 4980aacgggaaag
ttagtaatca ttcagaaatt tgtggtgaaa acgatgataa tcagtcagga
5040aataatcaca aatttgctgt acatactaca ccaatacaga ctgcatccga
taaacattca 5100ggagcagtgg cctcagcaat attaattcca ctactattag
tattccttgg agcagtattt 5160tattatttgc taaaaaggag aggtgatact
ggattaaata taagcatgcg attttataac 5220ccactctacg gaaaacctgt
ccgtgaagac caaaaacaaa tccttaaacc aggacaacac 5280gagtacacaa
atccgataat tgtggccgaa gaacatgagg accacgcgaa agatgctagc
5340agaatgctaa acgatagttg tgtttgttaa gtttgtatct ttttgttacg
tcattctttg 5400ttttatgttt tatataatct tataaacgtt gttttaatct
tttgaagtgt attaaaagca 5460aaatgactaa ttccattgtt gaacatagca
atctatatta acatcaaaat agatacaaat 5520tattatttat ctcttatatc
aaacagtaga attaaaactt aagaggaaac agtggcgatc 5580aacaggtggc
gaaggcgcgt tccaggattg cg 561225313DNADiabrotica virgifera
2atgttaattt tgttctttgt gctttcaacg ttattaaatg cagtcgtttc aacgaataat
60aatgattgcc ctccacatca gtttagatgc acaaattcaa aatgtataga ctttcaacaa
120agatgtgatg gttccgacaa ttgtggagat ggatcggatg aactggattg
tgatcataaa 180tgtcatgaac attttttcaa ttgtcgaaat ggaagatgta
taagcgaagc attcctgtgt 240gatggtgaaa atgattgtaa taactttttg
gatgaaaaag actgtaaggg tcaaacggtg 300ctaactctcg aaactgatgg
ccattgcgaa gatggtcatt ggaagtgtgc cgataaattg 360tgtattccac
ttgacatggt atgtaatggc gcacctgaat gtttggatgg atcagatgaa
420actattggat gcagtaacaa gatcgtttgt gatggattta aatgtaaaaa
tggccattgc 480ataccaactg aatgggcttg cgacggtctt gatgattgtg
gtgataaaac tgatgaacaa 540gattgcgcaa accacgtacc acttgatcaa
tgcactttgg ataaaagaaa attcttgtgt 600agtaacaata aaacttgcat
agatcttcac tcggtctgtg ataataagtt agattgtccc 660gattattctg
atgaaggaaa acagtgcaat tcgtctgcaa tatcatgtaa caataacaaa
720tgttctcata catgtatatc acttccaacg ggtccaaaat gtctgtgtcc
gaatggttac 780catacgcttg atgattcgaa ttgtttggat attaatgaat
gtacaactta tggaatttgt 840gaccaaaaat gtagaaatct tcctggatca
tacgaatgct actgtgatga taagtacaca 900ctccaagccg ataaaaaaac
atgtaaagca actggcggaa gcggaataat gatctttagt 960tccaagcagg
aaattcgagc tttaacctta gattctttag attattttac agtggctaaa
1020gatttaaatc aagtagtggg aatagcatac gatggacatc atatttactg
gacagacgta 1080tttactggac atgaaactat atcgaggtct attgaagatg
gatcggaaag ggaggtattg 1140gtaacatctg gactcagtct accagaggat
ctagcgtatg attggttaac tggtaatatt 1200tatttcaccg atgctctcaa
acaacatgtt ggagtgtgtt ccaatgatgg tcaccattgt 1260acagttctag
tcaacaaaga tattcgaaaa ccacgtggga ttgttgttaa tgtggaagct
1320ggagatatgt attggtctga ttggggtgta ccagcagcaa ttggatattc
acttatggat 1380ggatcccagg acaaaccgtt cgtcaccaat aatatacact
ggcccaatgg tttggctcta 1440gatcaaccta actctagact ttattggact
gatgctaaaa agatgacact agaaagtatt 1500aatttagacg gaactgatca
aaggatcgtt ttggaaggaa tagtgaaaca tccatatgcc 1560atcgctgtgt
ttgaaaacaa actttattgg tcagattggg actcccatac cattcaaact
1620tgtgataagt ttaatggtaa aaatcatcat acactcgtgg aagaacataa
aaatttaatc 1680tatggaatca gtatttttca ctatgctctc gaaaagcgtc
tagtgaatcc ttgtgagcat 1740gcttcctgta gtgacatttg tttattgaag
gctcagtcat atgcttgtgc gtgcccagag 1800aataaagttt tggatacgga
tggtcatacg tgcaaagaag tacaaccaac gcaagcactt 1860gtaggaggaa
ctggacacgt gttggtatca attaaacatc aatttctagg caaacacgat
1920gtaacattcc taccagacct ggcgaaacac gtaggaagct tagcatttga
tagttataaa 1980aacattttgt atgtaagcga tttggaaaca aaaagtatag
ttgcgcttaa catgaacatc 2040ggaattagca aaactcttga gatcgacggt
cttggcaaag ttacttcaat ggattttgat 2100ccaaaaagta ataatttata
catttgcgat acggagaggc tagtcgtaga aataattgat 2160atgaataatc
tagaaagaaa aataattgtc catgacacat ttggagaaac tcctgaaagc
2220attgcgttgg taccggaaga tgggatcatg tttgttagct ttaaacaagg
gaaattggga 2280aatagccaca tcgaccgttt tttcatggat ggtactggaa
gaactcacgt catcgaaaac 2340ggtttagtgg gcccagttcg tgtagtatac
gaccgcagtc tttatagaat tttctttgcc 2400gatctaagta ctggggtaat
agaatctact agcgcggccg gtgacgatag acatcatttt 2460cgtactctga
ctacccatcc agtaagtata gcagttttga aagatgacct attgtggaca
2520aatcttgact ccaaggattt atattggtct gaaaaaagat ctagcgagtc
ttatgagaag 2580aaaattacga taggttttaa agaggataac gtcaacatcc
acttagtgtc agtaacacgt 2640aaacaattag aaatcaatag ctgtcgtgtt
aataataatg gatgtagtca tctttgtctt 2700caatcccata aatcgatagt
ttgtgcatgt ccagctggat gggagctatc ggcaaatggt 2760tttacatgta
ataaaagggt aacctgcgac aataaagaga tgctttgtcc tcattccaat
2820acttgcgttc tgaagagctt aaggtgcaat ggttttaaag actgcgcatt
tggtgaagat 2880gaatgggact gccaaactgt ttcccaatgt ttacctggcc
agtataaatg cgacgatgga 2940cagtgcatca gtgaagattt ggtctgcaat
catagttatg actgtaaaga taagtctgat 3000gagtatggat gtgcagataa
aaacaaaaaa ctaggatgtc ctcccggaca ttttacctgt 3060aaaagctccg
aatgcatatc tgaacgcttt gtatgtgatg ctttccatga ttgcgatgat
3120ggatcagatg aactaaactg cgaaaataat atttgtttgg aatctcagtt
taggtgtgat 3180gtcggaactt gcataccaaa agattgggaa tgtgatggag
aatatgactg tactgatagt 3240tcagatgagc attgttcttc agaagtttgt
cagtctaact attttaagtg tgacaacaac 3300agatgtatcg atcctaagct
tcagtgcgat gggtttgatg actgcggtga tcactctgat 3360gaaaaattcg
agaaatgttt gcatcactcg aaagcaccaa aatgtacatt ggaagaattc
3420gcgtgtatta ctaatacttc catttgtctt ccaaaatcag ctaaatgtaa
cggaacgtca 3480gaatgtccga ataacgaaga cgaaaaggat tgttcaaaat
gtgaagaaga tgaatttgag 3540tgcaagcata agcattatag agaatgtatt
cctaggtcct ggatatgtga tggtacagat 3600gattgcggtg ataatagtga
tgagagtatg gaaacctgtt cttcaaaact taaacctgcg 3660gcagataact
ttgctgttac tgactcgtgt ctaaccggat atagatgtca tagcggtgca
3720tgcataaata tgacctcagt atgcaataat aaccacgatt gttttgatgg
ctctgatgaa 3780gatggcctct gttcttcatc ttgtgtcggt gtgaaaaatc
cttgcaatca aatatgcgta 3840aaaacaccat ctggaccaag atgtgaatgt
aaacctggat ataagttgtt aggagatgga 3900aaaacttgta ttgatgataa
tgaatgccaa acggatccac caatctgtag tcagctatgt 3960cacaataaag
aaggaggcta ttcttgcgat tgttacaata atttcctttt aagttctaat
4020aaaaaatctt gtaaagctca cggaccaaga atgaccatat acttagtgat
ttacggaaac 4080caaatccgtc acctaattcc taaatcaaac acaatggctg
ttctttacac caatccaatg 4140ataaaaatat ccagcttgga tactctggtt
aaaccaaaac taatcttctt cagttcttat 4200gaaactcaag ctatttataa
acttgatacc accaccaaca tgatgcacta catcagaaac 4260gtaggtttcc
ctagaacaat tgcagttgat tggtcaactc aaaacattta ttacttcgat
4320actgatgtta atggccggtc cattagtgtt tgcagttttg aagaaaagtg
cgctaagttg 4380ataaatattg aatcgcctcg acatgtaact gccttagctg
ttgattccgt caataaattg 4440cttttctacg ttttgaggaa ttggtgggta
ttagaaacac caagcttcgt aatatacagt 4500gtaaacttgg atggatctaa
tagacaagaa atagtaaaaa caacaacggg caatgttgaa 4560gatataactt
tcgatattaa caagaaactt ttatactata ctaacaaaca agatgaaagt
4620atacacgaag taagctacaa aggaggaccg gttaaaactg tattttcaaa
tatctctcaa 4680ccagaaggac ttaaattttt tgagaaccaa ttatactata
ctgttgcaac tggttatata 4740gtcagttgta agctatatgg agacagatcc
tgcaatccca gatataaatt acacacgttt 4800gccagtggtc aatttgttat
tgagcaggaa tcgcttcagc ctaaagtaga aaatgtttgt 4860actggacaca
aatgtccata cttatgtgta ccagcagaat caggatatag atgtctctgt
4920cataacggga aagttagtaa tcattcagaa atttgtggtg aaaacgatga
taatcagtca 4980ggaaataatc acaaatttgc tgtacatact acaccaatac
agactgcatc cgataaacat 5040tcaggagcag tggcctcagc aatattaatt
ccactactat tagtattcct tggagcagta 5100ttttattatt tgctaaaaag
gagaggtgat actggattaa atataagcat gcgattttat 5160aacccactct
acggaaaacc tgtccgtgaa gaccaaaaac aaatccttaa accaggacaa
5220cacgagtaca caaatccgat aattgtggcc gaagaacatg aggaccacgc
gaaagatgct 5280agcagaatgc taaacgatag ttgtgtttgt taa
53133236DNADiabrotica virgifera 3tgttaatttt gttctttgtg ctttcaacgt
tattaaatgc agtcgtttca acgaataata 60atgattgccc tccacatcag tttagatgca
caaattcaaa atgtatagac tttcaacaaa 120gatgtgatgg ttccgacaat
tgtggagatg gatcggatga actggattgt gatcataaat 180gtcatgaaca
ttttttcaat tgtcgaaatg gaagatgtat aagcgaagca ttcctg
2364155DNADiabrotica virgifera 4gctttcaacg ttattaaatg cagtcgtttc
aacgaataat aatgattgcc ctccacatca 60gtttagatgc acaaattcaa aatgtataga
ctttcaacaa agatgtgatg gttccgacaa 120ttgtggagat ggatcggatg
aactggattg tgatc 1555250DNADiabrotica virgifera 5gtcaccaata
atatacactg gcccaatggt ttggctctag atcaacctaa ctctagactt 60tattggactg
atgctaaaaa gatgacacta gaaagtatta atttagacgg aactgatcaa
120aggatcgttt tggaaggaat agtgaaacat ccatatgcca tcgctgtgtt
tgaaaacaaa 180ctttattggt cagattggga ctcccatacc attcaaactt
gtgataagtt taatggtaaa 240aatcatcata 2506155DNADiabrotica virgifera
6agaggataac gtcaacatcc acttagtgtc agtaacacgt aaacaattag aaatcaatag
60ctgtcgtgtt aataataatg gatgtagtca tctttgtctt caatcccata aatcgatagt
120ttgtgcatgt ccagctggat gggagctatc ggcaa 1557155DNADiabrotica
virgifera 7aatattaatt ccactactat tagtattcct tggagcagta ttttattatt
tgctaaaaag 60gagaggtgat actggattaa atataagcat gcgattttat aacccactct
acggaaaacc 120tgtccgtgaa gaccaaaaac aaatccttaa accag
1558400DNADiabrotica virgifera 8cctgtgtgat ggtgaaaatg attgtaataa
ctttttggat gaaaaagact gtaagggtca 60aacggtgcta actctcgaaa ctgatggcca
ttgcgaagat ggtcattgga agtgtgccga 120taaattgtgt attccacttg
acatggtatg taatggcgca cctgaatgtt tggatggatc 180agatgaaact
attggatgca gtaacaagat cgtttgtgat ggatttaaat gtaaaaatgg
240ccattgcata ccaactgaat gggcttgcga cggtcttgat gattgtggtg
ataaaactga 300tgaacaagat tgcgcaaacc acgtaccact tgatcaatgc
actttggata aaagaaaatt 360cttgtgtagt aacaataaaa cttgcataga
tcttcactcg 4009400DNADiabrotica virgifera 9gtctgtgata ataagttaga
ttgtcccgat tattctgatg aaggaaaaca gtgcaattcg 60tctgcaatat catgtaacaa
taacaaatgt tctcatacat gtatatcact tccaacgggt 120ccaaaatgtc
tgtgtccgaa tggttaccat acgcttgatg attcgaattg tttggatatt
180aatgaatgta caacttatgg aatttgtgac caaaaatgta gaaatcttcc
tggatcatac 240gaatgctact gtgatgataa gtacacactc caagccgata
aaaaaacatg taaagcaact 300ggcggaagcg gaataatgat ctttagttcc
aagcaggaaa ttcgagcttt aaccttagat 360tctttagatt attttacagt
ggctaaagat ttaaatcaag 40010368DNADiabrotica virgifera 10tagtgggaat
agcatacgat ggacatcata tttactggac agacgtattt actggacatg 60aaactatatc
gaggtctatt gaagatggat cggaaaggga ggtattggta acatctggac
120tcagtctacc agaggatcta gcgtatgatt ggttaactgg taatatttat
ttcaccgatg 180ctctcaaaca acatgttgga gtgtgttcca atgatggtca
ccattgtaca gttctagtca 240acaaagatat tcgaaaacca cgtgggattg
ttgttaatgt ggaagctgga gatatgtatt 300ggtctgattg gggtgtacca
gcagcaattg gatattcact tatggatgga tcccaggaca 360aaccgttc
36811475DNADiabrotica virgifera 11cactcgtgga agaacataaa aatttaatct
atggaatcag tatttttcac tatgctctcg 60aaaagcgtct agtgaatcct tgtgagcatg
cttcctgtag tgacatttgt ttattgaagg 120ctcagtcata tgcttgtgcg
tgcccagaga ataaagtttt ggatacggat ggtcatacgt 180gcaaagaagt
acaaccaacg caagcacttg taggaggaac tggacacgtg ttggtatcaa
240ttaaacatca atttctaggc aaacacgatg taacattcct accagacctg
gcgaaacacg 300taggaagctt agcatttgat agttataaaa acattttgta
tgtaagcgat ttggaaacaa 360aaagtatagt tgcgcttaac atgaacatcg
gaattagcaa aactcttgag atcgacggtc 420ttggcaaagt tacttcaatg
gattttgatc caaaaagtaa taatttatac atttg 47512474DNADiabrotica
virgifera 12cgatacggag aggctagtcg tagaaataat tgatatgaat aatctagaaa
gaaaaataat 60tgtccatgac acatttggag aaactcctga aagcattgcg ttggtaccgg
aagatgggat 120catgtttgtt agctttaaac aagggaaatt gggaaatagc
cacatcgacc gttttttcat 180ggatggtact ggaagaactc acgtcatcga
aaacggttta gtgggcccag ttcgtgtagt 240atacgaccgc agtctttata
gaattttctt tgccgatcta agtactgggg taatagaatc 300tactagcgcg
gccggtgacg atagacatca ttttcgtact ctgactaccc atccagtaag
360tatagcagtt ttgaaagatg acctattgtg gacaaatctt gactccaagg
atttatattg 420gtctgaaaaa agatctagcg agtcttatga gaagaaaatt
acgataggtt ttaa 47413500DNADiabrotica virgifera 13atggttttac
atgtaataaa agggtaacct gcgacaataa agagatgctt tgtcctcatt 60ccaatacttg
cgttctgaag agcttaaggt gcaatggttt taaagactgc gcatttggtg
120aagatgaatg ggactgccaa actgtttccc aatgtttacc tggccagtat
aaatgcgacg 180atggacagtg catcagtgaa gatttggtct gcaatcatag
ttatgactgt aaagataagt 240ctgatgagta tggatgtgca gataaaaaca
aaaaactagg atgtcctccc ggacatttta 300cctgtaaaag ctccgaatgc
atatctgaac gctttgtatg tgatgctttc catgattgcg 360atgatggatc
agatgaacta aactgcgaaa
ataatatttg tttggaatct cagtttaggt 420gtgatgtcgg aacttgcata
ccaaaagatt gggaatgtga tggagaatat gactgtactg 480atagttcaga
tgagcattgt 50014500DNADiabrotica virgifera 14tcttcagaag tttgtcagtc
taactatttt aagtgtgaca acaacagatg tatcgatcct 60aagcttcagt gcgatgggtt
tgatgactgc ggtgatcact ctgatgaaaa attcgagaaa 120tgtttgcatc
actcgaaagc accaaaatgt acattggaag aattcgcgtg tattactaat
180acttccattt gtcttccaaa atcagctaaa tgtaacggaa cgtcagaatg
tccgaataac 240gaagacgaaa aggattgttc aaaatgtgaa gaagatgaat
ttgagtgcaa gcataagcat 300tatagagaat gtattcctag gtcctggata
tgtgatggta cagatgattg cggtgataat 360agtgatgaga gtatggaaac
ctgttcttca aaacttaaac ctgcggcaga taactttgct 420gttactgact
cgtgtctaac cggatataga tgtcatagcg gtgcatgcat aaatatgacc
480tcagtatgca ataataacca 50015251DNADiabrotica virgifera
15cgattgtttt gatggctctg atgaagatgg cctctgttct tcatcttgtg tcggtgtgaa
60aaatccttgc aatcaaatat gcgtaaaaac accatctgga ccaagatgtg aatgtaaacc
120tggatataag ttgttaggag atggaaaaac ttgtattgat gataatgaat
gccaaacgga 180tccaccaatc tgtagtcagc tatgtcacaa taaagaagga
ggctattctt gcgattgtta 240caataatttc c 25116140DNADiabrotica
virgifera 16aaagtataca cgaagtaagc tacaaaggag gaccggttaa aactgtattt
tcaaatatct 60ctcaaccaga aggacttaaa ttttttgaga accaattata ctatactgtt
gcaactggtt 120atatagtcag ttgtaagcta 14017500DNADiabrotica virgifera
17tatggagaca gatcctgcaa tcccagatat aaattacaca cgtttgccag tggtcaattt
60gttattgagc aggaatcgct tcagcctaaa gtagaaaatg tttgtactgg acacaaatgt
120ccatacttat gtgtaccagc agaatcagga tatagatgtc tctgtcataa
cgggaaagtt 180agtaatcatt cagaaatttg tggtgaaaac gatgataatc
agtcaggaaa taatcacaaa 240tttgctgtac atactacacc aatacagact
gcatccgata aacattcagg agcagtggcc 300tcagcaatat taattccact
actattagta ttccttggag cagtatttta ttatttgcta 360aaaaggagag
gtgatactgg attaaatata agcatgcgat tttataaccc actctacgga
420aaacctgtcc gtgaagacca aaaacaaatc cttaaaccag gacaacacga
gtacacaaat 480ccgataattg tggccgaaga 50018488DNADiabrotica virgifera
18tcttgtaaag ctcacggacc aagaatgacc atatacttag tgatttacgg aaaccaaatc
60cgtcacctaa ttcctaaatc aaacacaatg gctgttcttt acaccaatcc aatgataaaa
120atatccagct tggatactct ggttaaacca aaactaatct tcttcagttc
ttatgaaact 180caagctattt ataaacttga taccaccacc aacatgatgc
actacatcag aaacgtaggt 240ttccctagaa caattgcagt tgattggtca
actcaaaaca tttattactt cgatactgat 300gttaatggcc ggtccattag
tgtttgcagt tttgaagaaa agtgcgctaa gttgataaat 360attgaatcgc
ctcgacatgt aactgcctta gctgttgatt ccgtcaataa attgcttttc
420tacgttttga ggaattggtg ggtattagaa acaccaagct tcgtaatata
cagtgtaaac 480ttggatgg 488195313DNADiabrotica undecimpunctata
19atgttaattt tgttttttgt gctttccacg ttattaaatg cagtcgtttc aacgaataac
60aatgattgtc ttcaacatca gttcagatgc acaaattcaa aatgtataga ttttcagcaa
120agatgtgatg gttccgataa ttgtggggat ggatcggatg aactggagtg
tgatcataaa 180tgtcatgaac attttttcga ttgtcgaaat gggagatgta
taagcgaagc attcgtatgt 240gatggtgaaa atgattgtaa taactttttg
gatgaaaaag actgtaagga taaaacgcta 300actctcgaaa ctgatcagca
ttgtgaagat ggtcattgga agtgtactga tagattgtgt 360attccaaatg
actgggtgtg taatggcgta cctgaatgtt tggatggatc agatgaaact
420attggatgta gcagcaagat cgattgtgat gggtttaaat gtaaaaatgg
tcattgtata 480ccaaatgaat ggaattgcga tggtgttgac gattgcggcg
ataaaactga tgaacaagat 540tgtgaagacc acgtgccact tgatcaatgc
actttggata aaagaaaatt cttgtgtagc 600aacaataaaa cttgcatcga
tcttcactca gtctgtgata ataagtttga ttgtcccgat 660tattctgatg
aaggaaaact ttgcaattcg tctacagtat tatgtaagaa taacaaatgt
720tcgcacacat gtatcccact tccaacgggt acaaaatgtc tgtgtccgaa
tggataccat 780acgattgatg attcgaaatg tttggatatt aatgaatgta
caatttatgg tatatgcgac 840caaaaatgtc gaaatcttcc tggatcatac
gaatgctact gtgatgataa atacaaactc 900caagctgata aaagaacttg
taaagcaatt ggaggaagcg gaataatgat ctttagttcc 960aagcaggaaa
ttagagcttt aaccttggat tctttagatt attttacagt ggctaaagat
1020ttaaaacaag tagtcggaat agcatacgat ggaaatcata tttattggac
agacgtattt 1080actggacatg aaactatatc gaggtctatt gaagatggat
cacagagaga ggtattggta 1140acatctggat taagtttacc agaggatcta
gcatttgatt ggttaactca taatatttat 1200tttaccgatg ctctgaaaca
acatgttgga gtatgttcca atgatggtaa ccattgtaca 1260gtactagtca
acaaagatat tcgaaaacca cgtgggattg ttgttaatgt ggaagctgga
1320gatatgtatt ggtctgattg gggagtacca gcagcaattg gatattcact
tatggatggt 1380tcccatgaca gaccgtttgt caccaataat atacactggc
ccaatggttt ggctctagat 1440caacctaact ctagacttta ttggactgat
gccaaaaaga tgacattaga aagtattaat 1500ttagacggaa ctgatcaaag
gattgttttg gaaggaatcg tgaaacatcc ctatgccatc 1560gctgtgtttg
aaaacaaatt gtattggtca gattgggact cccatagcat tcaaacttgt
1620gataagttcg atggtaaaaa tcaccataca ctcgtagaag aacataaaaa
tctaatctat 1680ggaatcagta tctttcacta tgctctcgaa aagcgtctag
tgaatccttg tgaccatact 1740tcctgtagtg atatttgtct attgaaggct
cagtcatatg cttgtgcgtg cccggaaaat 1800aaaattttag atacggatgg
tcatacgtgc aaagaaatac aaccaacgca agcacttgtt 1860ggaggaactg
gacatgtttt agtgtcaatc aaacatcaat ttctaggcaa acacgacgta
1920acattcctac cagacctggt gaaacacgta ggaagcttag cgtatgacag
ttataaaaaa 1980gttttgtatg ttagcgattt ggaaacaaaa agtatagttg
agcttaacat ggttacaggc 2040gttagcaaaa ctcttgatat cagcggtatt
ggcagagtta cttcaatgga ttttgatcca 2100aaaagtaata atttatacat
ttgcgatacg gaaaggctgg ttgtagaaat tattgatatg 2160aatagtcaag
aaagaaaaat aattgttcac gacacttttg gagaaagtcc tgaaagcatt
2220gctttggtac cggaagatgg tatcatgttt gtaagtttta aacaaggaaa
atcaggaaat 2280agtcacatcg atcgtttatt catggatggt accggaagaa
ctcacgtcat cgaaagcgga 2340ttagtgggcc cagtccatgt agtgtatgac
cacagtcttt atagaatttt ctttgcggat 2400ctaagtactg gtgttatcga
atctacgagt gtgaccggtg acgatagaca tcattttcgt 2460actctgacta
ctcatccagt aagtttagcg gttttgaaag atgatatatt gtggacaaat
2520attgactcca aggatttata ttggtctgaa aaaagatcta gtgagtctta
tgagaagaaa 2580attactttag gttttaaaga agataacgtt aatattcact
tagtatcagt gacacataaa 2640caattagaaa ttaatggttg ccgtgttaat
aataatggat gcagtcatct ttgtcttcaa 2700tcccacaaat caataatttg
tgcatgtcca gctggatggg agctatcggc aaatggtttt 2760acatgtaata
aaaggttaac ctgcgacaat aaagagatgc tttgtcctca ttccaatact
2820tgcattctaa agagcttaag gtgcaatggt tttaaagact gcgcatttgg
tgaagatgaa 2880tgggattgtc aagctgcttc cgaatgttta cctggtcagt
ataaatgcga cgatggacag 2940tgcatcagtg aagatttggt ctgcaatcat
agttatgact gtaaagataa gtcggacgag 3000tatggatgtg cagataaaaa
gaagaaacta gggtgtcctc ccggtcattt tacctgtaag 3060agctctgaat
gtatatcaga acgattttta tgtgatggtt atcatgattg cgatgatgga
3120tcagatgaat ttaattgcga gaataatatt tgtttggaat ctcagttcag
gtgtgatgtc 3180ggaacttgca taccaaaaga ttgggaatgc gatggagaat
atgactgtac tgataattca 3240gatgagcact gttcttcaga aggttgtcgc
tccgactatt tcaagtgtga taacaacaga 3300tgtatcgatc ctaaacttca
ttgcgatggg tttgatgact gcggtgacca ctctgatgaa 3360aaattcgagc
aatgtttgca tcactcgaaa gcaccaaaat gtacattgga ggaattcgca
3420tgcaccacta atacttccat ttgtcttcca caatcagcta aatgtaacgg
aacatcagaa 3480tgcccgaata aagaagacga aaaggattgt tcaaaatgcg
aagaagatga atttgagtgt 3540aagaataagc attacagaga atgtattcct
agatcctggt tatgtgatgg cgcagatgat 3600tgcggtgata atagtgatga
gaatatggaa acatgttctt caaaactcaa aactctggga 3660aataatactg
ctgttgctga tccgtgtcta accggatata gatgtcgcag cggtgcatgc
3720ataaatatga cttcagtatg caataataaa cacgattgtt atgatggctc
tgatgaagat 3780ggactctgtt cttcatcttg tgtcggtgta aaaaatccct
gcaatcaaat atgcgtaaaa 3840acaccatctg gaccaagatg tgaatgtaga
ctgggatata aattgctagg agacggaaaa 3900acgtgtatcg atgataatga
atgccaaacg gatcccccaa tctgtagtca gttatgtcac 3960aataaagaag
gaggttattc ttgtgattgt tacaataatt tccttttaag ttctaataaa
4020aaatcttgta aggctcacgg accacgaatg accatatact tagtgattta
cggaaacgaa 4080atccgtcaac taattcctaa atcaaataca atggctgttg
tttacaccaa tccaatgata 4140aaaatctcca gcttggatac tctgattaaa
ccaaaactaa tcttctttgg ttcttatgaa 4200actcaagcta tttataaact
ggataccacc acaaacatga tgcactacat cagaaacgta 4260ggttttccta
gaaaaattgc agttgattgg tccactcaaa acatttatta cttcgatact
4320gattataatg gccggtccat tagtgtttgc agttttgaag aaaagtgtgc
taagttaata 4380aatattgaat cgcctcgaca tgtatctgcc ttagctgttg
attccgttaa taaaaaactt 4440ttctacgttt tgaggaattg gtgggtatta
gaaacaccaa gcttcgtaat atacagtgta 4500aacttggatg gatctaatag
acaagaaata gtaaaaacaa caacaggtaa tgttgaagat 4560ataactttcg
atattaacaa gaaactttta tactacacta ataaacaaga tgaaagtata
4620tatgaagtaa actacaaagg aggacctgtt aaaactgtat tttcaaacat
ctctcaacca 4680gaaggtctta aatttttcga aaatcagtta tactatactg
ttgcaactgg ttatatagtc 4740agttgtaagc tatatggaga caaatcttgt
catcccagat ataaattaca cacttttgcc 4800agtggtcaat ttattattga
gcaggaatcg cttcagccta aaaccgaaaa tgtttgtact 4860ggacacaaat
gtccatactt atgtgtacca gcagaaacag gttatagatg tctttgccat
4920gacgggaaag ttagtaatca ttcagaaatt tgtggcgaaa atgatgataa
tcagtcagga 4980aataatcaca agtttgctgt acacactaca ccattacaaa
cggcatccga taaacactct 5040ggagcagtgg cctcagcaat attaattcca
atactcttgg tattccttgg agcagtattt 5100tattatgtgc taaaaaagaa
aggtgatact ggcttaaata taagcatgag attttataac 5160ccactttacg
gaaaacctgt cgaagaccaa aaaccaatcc ttcagccagg acaacacgag
5220tacacaaatc cgatcattgt ggcagaagaa agaagtcaag aggaccatgc
gaaagatgct 5280agcagaatgc taaacgatag ttgtgtttgt taa
5313205730DNADiabrotica undecimpunctata 20tttggccagt tcatcagtta
aaactgtgag tgctacattt tttgaatatc tcgtaaagta 60tgttaatttt gttttttgtg
ctttccacgt tattaaatgc agtcgtttca acgaataaca 120atgattgtct
tcaacatcag ttcagatgca caaattcaaa atgtatagat tttcagcaaa
180gatgtgatgg ttccgataat tgtggggatg gatcggatga actggagtgt
gatcataaat 240gtcatgaaca ttttttcgat tgtcgaaatg ggagatgtat
aagcgaagca ttcgtatgtg 300atggtgaaaa tgattgtaat aactttttgg
atgaaaaaga ctgtaaggat aaaacgctaa 360ctctcgaaac tgatcagcat
tgtgaagatg gtcattggaa gtgtactgat agattgtgta 420ttccaaatga
ctgggtgtgt aatggcgtac ctgaatgttt ggatggatca gatgaaacta
480ttggatgtag cagcaagatc gattgtgatg ggtttaaatg taaaaatggt
cattgtatac 540caaatgaatg gaattgcgat ggtgttgacg attgcggcga
taaaactgat gaacaagatt 600gtgaagacca cgtgccactt gatcaatgca
ctttggataa aagaaaattc ttgtgtagca 660acaataaaac ttgcatcgat
cttcactcag tctgtgataa taagtttgat tgtcccgatt 720attctgatga
aggaaaactt tgcaattcgt ctacagtatt atgtaagaat aacaaatgtt
780cgcacacatg tatcccactt ccaacgggta caaaatgtct gtgtccgaat
ggataccata 840cgattgatga ttcgaaatgt ttggatatta atgaatgtac
aatttatggt atatgcgacc 900aaaaatgtcg aaatcttcct ggatcatacg
aatgctactg tgatgataaa tacaaactcc 960aagctgataa aagaacttgt
aaagcaattg gaggaagcgg aataatgatc tttagttcca 1020agcaggaaat
tagagcttta accttggatt ctttagatta ttttacagtg gctaaagatt
1080taaaacaagt agtcggaata gcatacgatg gaaatcatat ttattggaca
gacgtattta 1140ctggacatga aactatatcg aggtctattg aagatggatc
acagagagag gtattggtaa 1200catctggatt aagtttacca gaggatctag
catttgattg gttaactcat aatatttatt 1260ttaccgatgc tctgaaacaa
catgttggag tatgttccaa tgatggtaac cattgtacag 1320tactagtcaa
caaagatatt cgaaaaccac gtgggattgt tgttaatgtg gaagctggag
1380atatgtattg gtctgattgg ggagtaccag cagcaattgg atattcactt
atggatggtt 1440cccatgacag accgtttgtc accaataata tacactggcc
caatggtttg gctctagatc 1500aacctaactc tagactttat tggactgatg
ccaaaaagat gacattagaa agtattaatt 1560tagacggaac tgatcaaagg
attgttttgg aaggaatcgt gaaacatccc tatgccatcg 1620ctgtgtttga
aaacaaattg tattggtcag attgggactc ccatagcatt caaacttgtg
1680ataagttcga tggtaaaaat caccatacac tcgtagaaga acataaaaat
ctaatctatg 1740gaatcagtat ctttcactat gctctcgaaa agcgtctagt
gaatccttgt gaccatactt 1800cctgtagtga tatttgtcta ttgaaggctc
agtcatatgc ttgtgcgtgc ccggaaaata 1860aaattttaga tacggatggt
catacgtgca aagaaataca accaacgcaa gcacttgttg 1920gaggaactgg
acatgtttta gtgtcaatca aacatcaatt tctaggcaaa cacgacgtaa
1980cattcctacc agacctggtg aaacacgtag gaagcttagc gtatgacagt
tataaaaaag 2040ttttgtatgt tagcgatttg gaaacaaaaa gtatagttga
gcttaacatg gttacaggcg 2100ttagcaaaac tcttgatatc agcggtattg
gcagagttac ttcaatggat tttgatccaa 2160aaagtaataa tttatacatt
tgcgatacgg aaaggctggt tgtagaaatt attgatatga 2220atagtcaaga
aagaaaaata attgttcacg acacttttgg agaaagtcct gaaagcattg
2280ctttggtacc ggaagatggt atcatgtttg taagttttaa acaaggaaaa
tcaggaaata 2340gtcacatcga tcgtttattc atggatggta ccggaagaac
tcacgtcatc gaaagcggat 2400tagtgggccc agtccatgta gtgtatgacc
acagtcttta tagaattttc tttgcggatc 2460taagtactgg tgttatcgaa
tctacgagtg tgaccggtga cgatagacat cattttcgta 2520ctctgactac
tcatccagta agtttagcgg ttttgaaaga tgatatattg tggacaaata
2580ttgactccaa ggatttatat tggtctgaaa aaagatctag tgagtcttat
gagaagaaaa 2640ttactttagg ttttaaagaa gataacgtta atattcactt
agtatcagtg acacataaac 2700aattagaaat taatggttgc cgtgttaata
ataatggatg cagtcatctt tgtcttcaat 2760cccacaaatc aataatttgt
gcatgtccag ctggatggga gctatcggca aatggtttta 2820catgtaataa
aaggttaacc tgcgacaata aagagatgct ttgtcctcat tccaatactt
2880gcattctaaa gagcttaagg tgcaatggtt ttaaagactg cgcatttggt
gaagatgaat 2940gggattgtca agctgcttcc gaatgtttac ctggtcagta
taaatgcgac gatggacagt 3000gcatcagtga agatttggtc tgcaatcata
gttatgactg taaagataag tcggacgagt 3060atggatgtgc agataaaaag
aagaaactag ggtgtcctcc cggtcatttt acctgtaaga 3120gctctgaatg
tatatcagaa cgatttttat gtgatggtta tcatgattgc gatgatggat
3180cagatgaatt taattgcgag aataatattt gtttggaatc tcagttcagg
tgtgatgtcg 3240gaacttgcat accaaaagat tgggaatgcg atggagaata
tgactgtact gataattcag 3300atgagcactg ttcttcagaa ggttgtcgct
ccgactattt caagtgtgat aacaacagat 3360gtatcgatcc taaacttcat
tgcgatgggt ttgatgactg cggtgaccac tctgatgaaa 3420aattcgagca
atgtttgcat cactcgaaag caccaaaatg tacattggag gaattcgcat
3480gcaccactaa tacttccatt tgtcttccac aatcagctaa atgtaacgga
acatcagaat 3540gcccgaataa agaagacgaa aaggattgtt caaaatgcga
agaagatgaa tttgagtgta 3600agaataagca ttacagagaa tgtattccta
gatcctggtt atgtgatggc gcagatgatt 3660gcggtgataa tagtgatgag
aatatggaaa catgttcttc aaaactcaaa actctgggaa 3720ataatactgc
tgttgctgat ccgtgtctaa ccggatatag atgtcgcagc ggtgcatgca
3780taaatatgac ttcagtatgc aataataaac acgattgtta tgatggctct
gatgaagatg 3840gactctgttc ttcatcttgt gtcggtgtaa aaaatccctg
caatcaaata tgcgtaaaaa 3900caccatctgg accaagatgt gaatgtagac
tgggatataa attgctagga gacggaaaaa 3960cgtgtatcga tgataatgaa
tgccaaacgg atcccccaat ctgtagtcag ttatgtcaca 4020ataaagaagg
aggttattct tgtgattgtt acaataattt ccttttaagt tctaataaaa
4080aatcttgtaa ggctcacgga ccacgaatga ccatatactt agtgatttac
ggaaacgaaa 4140tccgtcaact aattcctaaa tcaaatacaa tggctgttgt
ttacaccaat ccaatgataa 4200aaatctccag cttggatact ctgattaaac
caaaactaat cttctttggt tcttatgaaa 4260ctcaagctat ttataaactg
gataccacca caaacatgat gcactacatc agaaacgtag 4320gttttcctag
aaaaattgca gttgattggt ccactcaaaa catttattac ttcgatactg
4380attataatgg ccggtccatt agtgtttgca gttttgaaga aaagtgtgct
aagttaataa 4440atattgaatc gcctcgacat gtatctgcct tagctgttga
ttccgttaat aaaaaacttt 4500tctacgtttt gaggaattgg tgggtattag
aaacaccaag cttcgtaata tacagtgtaa 4560acttggatgg atctaataga
caagaaatag taaaaacaac aacaggtaat gttgaagata 4620taactttcga
tattaacaag aaacttttat actacactaa taaacaagat gaaagtatat
4680atgaagtaaa ctacaaagga ggacctgtta aaactgtatt ttcaaacatc
tctcaaccag 4740aaggtcttaa atttttcgaa aatcagttat actatactgt
tgcaactggt tatatagtca 4800gttgtaagct atatggagac aaatcttgtc
atcccagata taaattacac acttttgcca 4860gtggtcaatt tattattgag
caggaatcgc ttcagcctaa aaccgaaaat gtttgtactg 4920gacacaaatg
tccatactta tgtgtaccag cagaaacagg ttatagatgt ctttgccatg
4980acgggaaagt tagtaatcat tcagaaattt gtggcgaaaa tgatgataat
cagtcaggaa 5040ataatcacaa gtttgctgta cacactacac cattacaaac
ggcatccgat aaacactctg 5100gagcagtggc ctcagcaata ttaattccaa
tactcttggt attccttgga gcagtatttt 5160attatgtgct aaaaaagaaa
ggtgatactg gcttaaatat aagcatgaga ttttataacc 5220cactttacgg
aaaacctgtc gaagaccaaa aaccaatcct tcagccagga caacacgagt
5280acacaaatcc gatcattgtg gcagaagaaa gaagtcaaga ggaccatgcg
aaagatgcta 5340gcagaatgct aaacgatagt tgtgtttgtt aactttgtat
ctttttgtta cgtcattctt 5400tgttttatgt tttatataat cttataaacg
ttgttttaat cttttgaagt gtattaaaag 5460taaattaatt cttacacttc
acaaatatta aattcgttga aaattgaatt gttgaacata 5520acaatctata
ttatacctac atcaaaatag atacaaatga atatttatta taacgctata
5580tcgagcagta gagtttaacc ttaataccaa taaataggtg taacgggtgc
atttgacaat 5640atcaaattat ttattataga ataggctaaa tgcataatac
tttatatttt tataattata 5700ttttattaaa attttatgaa ctttaaaaaa
5730215295DNALeptinotarsa decemlineata 21atgtatgttc ttttggttct
gggagctgta gcatcctcat cagctttctt ggaatcgcta 60atctccctga acgactgccc
tccggctcag tttgcttgca ggaatggaaa atgcgtggat 120aaaactttga
gatgcaatgg attcaacaac tgtttggatg gatctgatga aatggattgt
180gaattgtatt tatgcaaaga gccgagatat tatcgatgta agaataaaag
atgtatcagc 240aaattatttg tttgcgatgg agaaaatgat tgcgaggatt
tttctgatga agtcgaatgt 300gagaacttta agatgtcaga tcacgtgaac
acaacatgcg aaaaaggcca ctggcagtgc 360acagataaac tgtgcatacc
aaatgaatgg gtgtgcaatg gagaatcaga ttgtttagat 420ggatcagatg
aaggaatagg gtgcagctcg aaaatagagt gcgatggatt caaatgtaaa
480aatggtcact gtattcccaa cgaatggata tgcgacaaca acaatgactg
tcacgataat 540agtgacgagg aggattgtga aaatcacgtt cctatcaaca
agtgcacact ggataatcga 600aaatttcttt gctctaacaa taaaacctgc
attgacctag ttcttacgtg cgatggccat 660cctaactgcc cggatggatc
tgacgaggga cccctttgca aatcggcagt ttcttgccaa 720aatcacgggt
gctctcacga atgtttccct ttgcctacag gaccgttatg tttatgccct
780cctggatatc ttactgagaa tgataaacgt tgccaggata tcaacgagtg
cgaacagttt 840gatatttgtg atcaaaagtg caggaatctg ccgggatcct
atgaatgtta ttgcgatcat 900aaatatttgc ttaaagaaga taaaagaact
tgcaaagctg tgggtggaga agctttaatg 960gtatttagtt ccaaatccga
aataagagca tatgccttgg aatccgagtt atattttccg 1020atagtacaaa
agttgaagca agtagtcggc gttgattatg acggccacca tatctactgg
1080acggatattt ttgcagagca tgagagtatc gcacgaacca ctgaggatgg
atcaaaacga 1140gagattctga ttacttccgg acttggttta ccagaagatt
tagctgttga ttggctcaca 1200ggaaatattt atttcacgga tgctgaaaag
gcgcatatag gcgtttgtaa cagtcatgga 1260actcactgta ccgttttggt
gaacaaagat gtcaatagac ccagaggact ggtactgaat 1320gttgaagagg
gagacatgta ctggtctgat tggggaaaac cgtccaaaat tgctcattct
1380ctgatggatg gttcaaatga cagagcattc atatctacag atattcattg
gcctaatggg 1440ctagccctgg atcaatctaa
ccaaaggctt tattggactg atgctaaaaa aatgaccctg 1500gaaagcattc
gattggacgg aacggataga aggattgtgt tggaaggggt cgtgaaacat
1560ccttatgcca ttgcggtgtt cgaaaataaa ttgtactggt cagattggtc
aactcgttcc 1620atccaaactt gtgacaaatt tactggcaaa gatcatcaca
ctctggttaa agaagataag 1680tatatatatg gaatcagcat attccattca
gctctccaca gaagacttga taatccttgt 1740gcattgtcct actgtagcga
tatttgcttg ctgaagggaa atacctacag ctgtgcctgc 1800cctgagaata
aacttctggg aagtgacaag cattcatgta aagatattgg acccacagaa
1860acgttgatcg ctgggactca agatagttta gttcatatag agcacaaatt
tttgggaaaa 1920catgatgtca cgtatctgcc cagtatagtt aaaaaaattg
gttgcttggc ttatgatagt 1980attaataact cactactgat cagcgacttg
gagttgaaaa aaatcgttaa cttggatttg 2040gatgatggaa tcaataaagt
tcttgaaatt gatggattag gaagtataac agcaatggat 2100tatgatgcta
aaggaaataa cctctatgta tgtgataggg agaaacgaac cttggaagta
2160atcagtctca atactttgtc aaaaaaaatt cttctgaact tcatgaacgg
ggaaattcca 2220gaagctgtgg ctgtggttcc ggatgaaggg gtgatgtttg
tcagtttgag agaagcagaa 2280ggtacatttt gtcatttgga tcgcttctat
atggacggaa cgggaaggac acacacctta 2340gaagatcatc tgataggccc
tatatcacta cgctatgacc atgatttgaa cagagttttc 2400tttgctgatg
ctggtactgg attgatagaa tcaactagtg ttgaaggaga cgatcgacat
2460gatttccgaa ggctaacaac ccacccctct agcatcacaa ctcttaaaaa
tgatctattc 2520tgggtgaacg aaaactccaa gcgtttgtat tgggcagaga
agaaaagcat ttcaatgtat 2580aacagaaaaa ttaccctaga tctctccaga
gaaccagaga aactccatct aatatcagta 2640acccccagaa aaaatgtgtt
gagcgcttgt tggctcaaca acaatggatg cagtcacctt 2700tgcctcatgt
cgcacaagac aacggtttgc gcttgtccaa ctggctacga actatccgac
2760gaccacagga cttgcatccg acgcgtgcat tgcgactctt ctgaattctt
ttgccctcag 2820tctaacgtct gcgtcatgaa gagcttaaga tgcaattcac
atcaggactg tgcatttgga 2880gaagatgaac tgaactgtga cataaacaga
tgcacaaccg ggttcttccg ttgcgataat 2940ggcgattgca tcaaagaaca
gctagtgtgc gaccatcatt acgactgcag ggataaatct 3000gacgaagata
attgcgctga gaagaagaaa cacgtgggat gtccgccaaa gcactttaga
3060tgcgggaatg gagactgcat ttcagataga ttcatatgcg atggttataa
agattgtgag 3120gatagctcgg atgaaaaggg ctgcgagaag atgttctgtt
cggataccga atttagatgc 3180gattctggaa catgtattcc tagtagctgg
gagtgcgacc acgaatatga ttgctcagat 3240tcctccgacg aacatcccgg
atgtgcttcc atcacctgtt ctgcaaccat gttcacttgt 3300ggaaatggca
aatgcctcga taaaagtctt gtctgcgaca aaagcgatga ctgtggcgat
3360aattcagacg aaacgtcctg cttcaaacgc ccctcctctt gccttccgca
agaattttct 3420tgtccttcga atacctctat ctgcgtccct atgtcagcca
agtgcaatgg atccgtcgag 3480tgtcctaagg gagaagacga ggaagaatgt
ggcgactgca tgccggatga gttccgctgt 3540ctcaacaagc aatgcattgc
ggcacaatgg atgtgcgatg gcatcaacga ctgtgatgac 3600ggcagtgatg
aaactgctga aggttgctcg cagaagaata aaaccacggg ggagatgtcc
3660gtggttcata ctgcctgtga aaatggattc aggtgtaaaa atggagattg
cttggatatg 3720aaactggtgt gtaatggaaa tcacgattgt tacgatggtt
ctgatgagga tggactgtgt 3780tcaacttcct gtgaaggatc agccaatcga
tgttctcaaa tatgcaataa aactccaatg 3840ggaccgtctt gcgagtgtag
atctgggttt atattgatgg gcaatggggt cagctgcatg 3900gatttgaatg
aatgccagaa tcatccacca gtgtgcagtc aaatttgcca caatcaggac
3960ggcggtttca aatgcgattg ttatgatggc tttttactga gatccgacaa
gaaatcttgc 4020aaagcagaag gaaatccgat gtcgctcatt ttcagtgaat
atgggcaaat aagggaactc 4080tcgcaattga ctaatatctt gtctgttgta
tttgccactc ctgcaccaaa aataactggc 4140ctagacgtac ttgtcaatcc
aagaaccatc tatttcagca tggaggaaac ttccacgatt 4200cacaaaatcg
atatggcatc aaaaacgaga catttcataa ggaatattgg acaacctcag
4260gacattgctg ttgattggtc tactcacaac atctattact ataacacgga
caccaactcg 4320aaatctatca gtatttgtag tttcgaagaa aagtgcgcga
agttgattga tgttgacatg 4380cacagacaag tggcaggatt ggttgtcgac
tctgtcaaca aatttttatt ttatgctttg 4440aacagttggt acgtgttcaa
ttcccccagt tacgttatct acaggtgcaa cttggatgga 4500actgagaaga
cggaaataat gaaaactaca gatgggtttg tatccgacat cacttttgac
4560cacaacagta ggatattgta ctacatggat atgtacttgg gtcaaataaa
taagataacc 4620tatgatggta gtttcaaaac tccaatttat tccaatatca
cccgatcctc agggcttaag 4680ttcttcgaga accgattata ctattctacg
aatgggggat ttgtttcagt ttgcaggtta 4740tacggatttc tgagatgtga
tagtttcagg cttcacaatg atgcaataga tttcttcact 4800attctacaag
agacgcttca gccgagtgtg ggtaatcctt gcaagaacca caattgctcc
4860aacttatgcg ttccagctca gtttggatat aaatgtctct gccaggatgg
aacgcttcta 4920tctggaccag gaagttgtcc aattaatgag aataccccag
agtccgacaa aaaatatact 4980gttcactcgg tggcgatgac acaggctggt
cctgaggagg gaagttcggg tactgtagct 5040gttgccatca cgatacctct
tctggtgata ttatttgctt tgggagccat ttactttatc 5100aagaaaaagc
atgcaggagc tttgaatgtc aggatgagat tttatactcc tcatttcgga
5160aggaccgttc aagatgaaaa tcctattcta aagcctggtc aacatgaata
cacaaaccct 5220gttcatttta ataaagaaga taatgaatta actctagaaa
cagcacctat caaactgaat 5280gatgcctcga atgtg
5295225690DNALeptinotarsa decemlineata 22gctctcgtag ttagcccttc
tgtgttgtga aaattgtttc attttatcag tattcatcct 60tagaaattga tatttttttc
atatggtttt caaccgagat tgattactaa atatttgaga 120atgtatgttc
ttttggttct gggagctgta gcatcctcat cagctttctt ggaatcgcta
180atctccctga acgactgccc tccggctcag tttgcttgca ggaatggaaa
atgcgtggat 240aaaactttga gatgcaatgg attcaacaac tgtttggatg
gatctgatga aatggattgt 300gaattgtatt tatgcaaaga gccgagatat
tatcgatgta agaataaaag atgtatcagc 360aaattatttg tttgcgatgg
agaaaatgat tgcgaggatt tttctgatga agtcgaatgt 420gagaacttta
agatgtcaga tcacgtgaac acaacatgcg aaaaaggcca ctggcagtgc
480acagataaac tgtgcatacc aaatgaatgg gtgtgcaatg gagaatcaga
ttgtttagat 540ggatcagatg aaggaatagg gtgcagctcg aaaatagagt
gcgatggatt caaatgtaaa 600aatggtcact gtattcccaa cgaatggata
tgcgacaaca acaatgactg tcacgataat 660agtgacgagg aggattgtga
aaatcacgtt cctatcaaca agtgcacact ggataatcga 720aaatttcttt
gctctaacaa taaaacctgc attgacctag ttcttacgtg cgatggccat
780cctaactgcc cggatggatc tgacgaggga cccctttgca aatcggcagt
ttcttgccaa 840aatcacgggt gctctcacga atgtttccct ttgcctacag
gaccgttatg tttatgccct 900cctggatatc ttactgagaa tgataaacgt
tgccaggata tcaacgagtg cgaacagttt 960gatatttgtg atcaaaagtg
caggaatctg ccgggatcct atgaatgtta ttgcgatcat 1020aaatatttgc
ttaaagaaga taaaagaact tgcaaagctg tgggtggaga agctttaatg
1080gtatttagtt ccaaatccga aataagagca tatgccttgg aatccgagtt
atattttccg 1140atagtacaaa agttgaagca agtagtcggc gttgattatg
acggccacca tatctactgg 1200acggatattt ttgcagagca tgagagtatc
gcacgaacca ctgaggatgg atcaaaacga 1260gagattctga ttacttccgg
acttggttta ccagaagatt tagctgttga ttggctcaca 1320ggaaatattt
atttcacgga tgctgaaaag gcgcatatag gcgtttgtaa cagtcatgga
1380actcactgta ccgttttggt gaacaaagat gtcaatagac ccagaggact
ggtactgaat 1440gttgaagagg gagacatgta ctggtctgat tggggaaaac
cgtccaaaat tgctcattct 1500ctgatggatg gttcaaatga cagagcattc
atatctacag atattcattg gcctaatggg 1560ctagccctgg atcaatctaa
ccaaaggctt tattggactg atgctaaaaa aatgaccctg 1620gaaagcattc
gattggacgg aacggataga aggattgtgt tggaaggggt cgtgaaacat
1680ccttatgcca ttgcggtgtt cgaaaataaa ttgtactggt cagattggtc
aactcgttcc 1740atccaaactt gtgacaaatt tactggcaaa gatcatcaca
ctctggttaa agaagataag 1800tatatatatg gaatcagcat attccattca
gctctccaca gaagacttga taatccttgt 1860gcattgtcct actgtagcga
tatttgcttg ctgaagggaa atacctacag ctgtgcctgc 1920cctgagaata
aacttctggg aagtgacaag cattcatgta aagatattgg acccacagaa
1980acgttgatcg ctgggactca agatagttta gttcatatag agcacaaatt
tttgggaaaa 2040catgatgtca cgtatctgcc cagtatagtt aaaaaaattg
gttgcttggc ttatgatagt 2100attaataact cactactgat cagcgacttg
gagttgaaaa aaatcgttaa cttggatttg 2160gatgatggaa tcaataaagt
tcttgaaatt gatggattag gaagtataac agcaatggat 2220tatgatgcta
aaggaaataa cctctatgta tgtgataggg agaaacgaac cttggaagta
2280atcagtctca atactttgtc aaaaaaaatt cttctgaact tcatgaacgg
ggaaattcca 2340gaagctgtgg ctgtggttcc ggatgaaggg gtgatgtttg
tcagtttgag agaagcagaa 2400ggtacatttt gtcatttgga tcgcttctat
atggacggaa cgggaaggac acacacctta 2460gaagatcatc tgataggccc
tatatcacta cgctatgacc atgatttgaa cagagttttc 2520tttgctgatg
ctggtactgg attgatagaa tcaactagtg ttgaaggaga cgatcgacat
2580gatttccgaa ggctaacaac ccacccctct agcatcacaa ctcttaaaaa
tgatctattc 2640tgggtgaacg aaaactccaa gcgtttgtat tgggcagaga
agaaaagcat ttcaatgtat 2700aacagaaaaa ttaccctaga tctctccaga
gaaccagaga aactccatct aatatcagta 2760acccccagaa aaaatgtgtt
gagcgcttgt tggctcaaca acaatggatg cagtcacctt 2820tgcctcatgt
cgcacaagac aacggtttgc gcttgtccaa ctggctacga actatccgac
2880gaccacagga cttgcatccg acgcgtgcat tgcgactctt ctgaattctt
ttgccctcag 2940tctaacgtct gcgtcatgaa gagcttaaga tgcaattcac
atcaggactg tgcatttgga 3000gaagatgaac tgaactgtga cataaacaga
tgcacaaccg ggttcttccg ttgcgataat 3060ggcgattgca tcaaagaaca
gctagtgtgc gaccatcatt acgactgcag ggataaatct 3120gacgaagata
attgcgctga gaagaagaaa cacgtgggat gtccgccaaa gcactttaga
3180tgcgggaatg gagactgcat ttcagataga ttcatatgcg atggttataa
agattgtgag 3240gatagctcgg atgaaaaggg ctgcgagaag atgttctgtt
cggataccga atttagatgc 3300gattctggaa catgtattcc tagtagctgg
gagtgcgacc acgaatatga ttgctcagat 3360tcctccgacg aacatcccgg
atgtgcttcc atcacctgtt ctgcaaccat gttcacttgt 3420ggaaatggca
aatgcctcga taaaagtctt gtctgcgaca aaagcgatga ctgtggcgat
3480aattcagacg aaacgtcctg cttcaaacgc ccctcctctt gccttccgca
agaattttct 3540tgtccttcga atacctctat ctgcgtccct atgtcagcca
agtgcaatgg atccgtcgag 3600tgtcctaagg gagaagacga ggaagaatgt
ggcgactgca tgccggatga gttccgctgt 3660ctcaacaagc aatgcattgc
ggcacaatgg atgtgcgatg gcatcaacga ctgtgatgac 3720ggcagtgatg
aaactgctga aggttgctcg cagaagaata aaaccacggg ggagatgtcc
3780gtggttcata ctgcctgtga aaatggattc aggtgtaaaa atggagattg
cttggatatg 3840aaactggtgt gtaatggaaa tcacgattgt tacgatggtt
ctgatgagga tggactgtgt 3900tcaacttcct gtgaaggatc agccaatcga
tgttctcaaa tatgcaataa aactccaatg 3960ggaccgtctt gcgagtgtag
atctgggttt atattgatgg gcaatggggt cagctgcatg 4020gatttgaatg
aatgccagaa tcatccacca gtgtgcagtc aaatttgcca caatcaggac
4080ggcggtttca aatgcgattg ttatgatggc tttttactga gatccgacaa
gaaatcttgc 4140aaagcagaag gaaatccgat gtcgctcatt ttcagtgaat
atgggcaaat aagggaactc 4200tcgcaattga ctaatatctt gtctgttgta
tttgccactc ctgcaccaaa aataactggc 4260ctagacgtac ttgtcaatcc
aagaaccatc tatttcagca tggaggaaac ttccacgatt 4320cacaaaatcg
atatggcatc aaaaacgaga catttcataa ggaatattgg acaacctcag
4380gacattgctg ttgattggtc tactcacaac atctattact ataacacgga
caccaactcg 4440aaatctatca gtatttgtag tttcgaagaa aagtgcgcga
agttgattga tgttgacatg 4500cacagacaag tggcaggatt ggttgtcgac
tctgtcaaca aatttttatt ttatgctttg 4560aacagttggt acgtgttcaa
ttcccccagt tacgttatct acaggtgcaa cttggatgga 4620actgagaaga
cggaaataat gaaaactaca gatgggtttg tatccgacat cacttttgac
4680cacaacagta ggatattgta ctacatggat atgtacttgg gtcaaataaa
taagataacc 4740tatgatggta gtttcaaaac tccaatttat tccaatatca
cccgatcctc agggcttaag 4800ttcttcgaga accgattata ctattctacg
aatgggggat ttgtttcagt ttgcaggtta 4860tacggatttc tgagatgtga
tagtttcagg cttcacaatg atgcaataga tttcttcact 4920attctacaag
agacgcttca gccgagtgtg ggtaatcctt gcaagaacca caattgctcc
4980aacttatgcg ttccagctca gtttggatat aaatgtctct gccaggatgg
aacgcttcta 5040tctggaccag gaagttgtcc aattaatgag aataccccag
agtccgacaa aaaatatact 5100gttcactcgg tggcgatgac acaggctggt
cctgaggagg gaagttcggg tactgtagct 5160gttgccatca cgatacctct
tctggtgata ttatttgctt tgggagccat ttactttatc 5220aagaaaaagc
atgcaggagc tttgaatgtc aggatgagat tttatactcc tcatttcgga
5280aggaccgttc aagatgaaaa tcctattcta aagcctggtc aacatgaata
cacaaaccct 5340gttcatttta ataaagaaga taatgaatta actctagaaa
cagcacctat caaactgaat 5400gatgcctcga atgtgtgata tttaataagt
ttctttgggt tttacttttt atattgttgt 5460tttatcgttt gtataagttt
tatcgatgtt aaatattgaa atgttatatt tttaccgtta 5520tagttcataa
atgaagagga atgcttcaga gtgaatattg aatttgaagt gtgatattga
5580tagcattgtt atacatttag ctattacaca ggatgaacaa tgtacacatc
ctccatatat 5640ttattatcat tgaaaaatga ataaatcatt ttaatagtac
ctactgaaaa 5690234413DNAPhyllotreta striolata 23atgttactcg
tattgctcct gggagcagta acagtctctt cgtcagcagt tttcccaccc 60aaaggaaatt
gcacctccga acaattccaa tgctccgatc gaagatgcat aagcctcaaa
120gccagatgcg acttgataat ggattgcccg gacggatcgg acgaattgga
atgcgatttg 180cagctctgcg atgagccgga gttcttccgc tgtaaaaata
acaaatgcat cagtcacgat 240ttggtctgcg ataaaatgaa cgattgcgac
gacttctccg acgagttgaa ctgccaaagc 300ttccagtctt tggtggcgtc
ggcttgcgag gagggcacct ggaggtgcgc cgataaattg 360tgcataccca
acgattgggt gtgcgacggc acgccggaat gcatcgacgg atccgacgaa
420accatcggtt gcagcaggca aatcgactgc gacgggttcc gctgcaagaa
caagcattgc 480gtgcccaaag agtgggtctg cgaccatcac aacgattgca
tcgataacag cgacgaagag 540aactgcgagg accacgtccc catcgaccag
tgcacgttgg acaagcgcaa gttcctctgc 600cacaacaacg tgacgtgcat
cgatacggaa tcggtttgcg acggacacaa ccactgtccg 660gacaattcgg
acgaatcgcc caattgcaac gcgtccgcca aggcgtgccg gggcgatcac
720aagtgctcgc acgtttgcat accgctgccc gccggcccca cgtgcctctg
ccccctcggc 780taccacacgc tcgacgagaa gaactgcgag gacatcaacg
aatgcgaaac atacggtata 840tgcgatcaaa tgtgccggaa cacgccgggc
tcgtacgagt gctactgcga caaggcgtac 900catttggcgg atgacaagaa
aacctgcaag gccgatggcg aggtgggcgt catggtgttc 960agttcgaagg
atcagatcag agcggtggcg ttgaatttga ccagttacat cctcgtcgct
1020aagaacttga agaaagtggt gggcgtcgat ttcgacggtc gcagggtcta
ctggacggag 1080atatacgggg gctacgagaa catcgcccgg agcgccgagg
acgggtcgca aaaggaggtt 1140ttagtaacgg ccggtataga tctgccggag
gatttggcca tcgactggtt gacgggtaac 1200atttatttca ccgatggcga
gaagaaacac ataggagttt gctcgcccga cgggacccat 1260tgcgctgttt
taatcaataa ggacatcgcg aaacccagag gcatcgttct taacgtcttg
1320gacggcgaca tgtattggac ggattgggaa gaaccgtcgc gaatcggtta
ctcattaatg 1380gacggatcaa atgataagcc cttcgtagaa aaagacattc
attggcccaa cggtttagca 1440ttggatcatc ctaatagcag actgtactgg
acggatgcta agaagatgac gttggaaagc 1500atccgtttag atggaactga
tcgaaggata atattagaag atatcgtaaa gcatccgtac 1560gccatagcgg
tattcgaaga taaattgtac tggtcggatt gggcgacgcg caccatccag
1620cagtgcgaca aattcgacgg gaagaatcac cggacgatga tcgaagagaa
ggaactcatc 1680tacggtatca gcatatacca tccggcgcaa cagtcgcgca
gcgtaaatcc ctgcgagacg 1740gccctgtgca gcgacctgtg cttgctgaaa
ggtacatcgt acagctgcgc ttgtccgcag 1800aacaaggtcc tacagcccga
cggaatgttt tgtaaagaat tgtcgcctat agagagtctc 1860atagccgctc
atcgggacat gttggtacac atcgagcatc cgattctcgg caggcatgtt
1920gttacagctc taccgggcgc cactggagac attgaaagct tggcattcga
tagcttcaaa 1980aacgtactgt acatcagcga tttcaagact aagagaatca
ccactttgca catgaagacc 2040ggtaccagca aagtaatgga tatacccgat
ttgggtagaa tcaacgctat ggatttcgat 2100tccaaaagca acaatcttta
catctgcgat tcggtccgca aagtggtaga agtaatcagt 2160atcaacacga
tggctcggcg aattttaatt cacgacacgt taggagagta cccgcaaagt
2220atcgcgttag ttcccgacga aggggtgatg tttataagct tcggaggcga
gggacgcagc 2280cacatcgatc gcttcttcat ggacggcacc ggccgaacgc
acgccatcga caccaaactg 2340acggggcccg tcgtgctcgc gtacgacgcc
gatttgcaca gggtcttctt cgccgacgcc 2400gccaacggcg tcatcgaatc
caccagcgtc gacggcgacg atcgcatcca cttcagaacc 2460gtcgacacgc
accccaccag cctggtggtc ctcaaagacg acgtgttctg gatgaacgcc
2520cgctcgaagc agctctactg gacgtcgaag aaagtcccct cgaactacga
caagaaaatc 2580acgctcgcct tccccgacga tccggacaag gtgcacctgg
cgtcggtgac gtcgcgccgc 2640aaggagacca acctctgtcg catcaacaac
aacggttgca gccacttgtg cgtccaatcg 2700cagaaatcga tcgtgtgccg
atgtccgatc ggttgggaac tgaaacagga caatcgcacg 2760tgcgccaagc
gagtgggttg catcagcgac gaatttctgt gcgcgcagtc caacgcgtgc
2820atcgtgaaga gcctgcgttg cgacggccgg aaggattgcc tcttcggcga
agacgaggac 2880gattgcgcgg cgacgaagac gtgcggcgac gggcagttcc
gatgcgccga cggcgattgc 2940atcgccgaag ggctggcgtg caacctgcgc
tacgattgca aggataaatc ggacgagcac 3000ggttgcggcg acgtgatgaa
cggcacgcga tgcgcgcccg atcacttcgc ctgctcgaac 3060ggcgaatgca
tcagcgggca tttccactgc gacggcgtct ccgattgcac ggacaatacg
3120gacgagttgc actgtcagac gaaggagtgc aatgtaacgc agtttaggtg
cgattcgggc 3180gcttgtattc ccaaggagtg ggagtgcgat caggattacg
attgcgcgga taattccgat 3240gaacattgcg atacggtatg cccggcgagc
cacttcaaat gcgacaacgg cctgtgcgtg 3300gataagaaat tattgtgcga
cggtttcgac aattgcggcg accattccga cgagaagatt 3360cacatgtgtc
accagagggc cccgcacaat tgcaccgcct acgagacggc ctgctcgtcc
3420aacgcttcca tttgcattcc tttgggagcg aaatgcgacg gcaaatccga
ctgtcccaag 3480cacgaggacg agatcggctg cgccatgtgc gccgacgagg
atttcgaatg ccgcagcaaa 3540cacctcaagg actgcattcc gagattgtgg
ttgtgcgacg gcgagaacga ttgcggcgac 3600aacagcgacg aggacttggt
gatgtgcagt aaaaagaaca agaccacgtt cgctccggcc 3660caacaaccct
gcaccgacgg cttcagatgc gacaacggcg tttgcatcaa ctctacgttg
3720ctctgtaacg gcaaacacga ctgctacgac ggctccgacg aaggaggctc
ctgctccggg 3780tcctgcgacg gccccaagaa tccctgcaac cacgtctgcg
tcaagacgcc gaagggaccg 3840aagtgccggt gcagaccggg ctaccggttg
atgggcgacg gtaaaacgtg cgtcgacgtg 3900aacgagtgcg aggccgatcc
gccgatatgc agccaacttt gccgcaacaa agatggcggt 3960tacacgtgcg
attgtttcca aaacttttat ttgaggaaga acatgaaatc gtgcaaagcg
4020gccggttccg acatgttgat ctacttcaac gtgaacggtg acgaaatcta
cgaaatctcg 4080ccgaagaaca attcgatgag cgtcgtccag gacgggtcga
tgttgaagat ctcgagtctg 4140gccgccgtgg tggactcgaa cgagctgttc
tacagcgtac ccgacaccgg cgccgtctac 4200aagctcgatc cggccaccaa
aaccgttcgc tacatcgaga acttgggcga gcccaaactg 4260atagcggtgg
attgggcgac gcgtaatgtt tattattacg acgccgaatc ggacgcgaaa
4320tcgattggag tgtgcaattt cgaggagaag tgcgtcaaat tgatcgacgt
cgatgctcat 4380cggcaggtgt ccgccttgac cgtggattca taa
4413245529DNAPhyllotreta striolata 24aactcgtgtt acttaccttt
gaaaacgatc agtttcacgt ggcctgctcg aaataattga 60gagaatgtta ctcgtattgc
tcctgggagc agtaacagtc tcttcgtcag cagttttccc 120acccaaagga
aattgcacct ccgaacaatt ccaatgctcc gatcgaagat gcataagcct
180caaagccaga tgcgacttga taatggattg cccggacgga tcggacgaat
tggaatgcga 240tttgcagctc tgcgatgagc cggagttctt ccgctgtaaa
aataacaaat gcatcagtca 300cgatttggtc tgcgataaaa tgaacgattg
cgacgacttc tccgacgagt tgaactgcca 360aagcttccag tctttggtgg
cgtcggcttg cgaggagggc acctggaggt gcgccgataa 420attgtgcata
cccaacgatt gggtgtgcga cggcacgccg gaatgcatcg acggatccga
480cgaaaccatc ggttgcagca ggcaaatcga ctgcgacggg ttccgctgca
agaacaagca 540ttgcgtgccc aaagagtggg tctgcgacca tcacaacgat
tgcatcgata acagcgacga 600agagaactgc gaggaccacg tccccatcga
ccagtgcacg ttggacaagc gcaagttcct 660ctgccacaac aacgtgacgt
gcatcgatac ggaatcggtt tgcgacggac acaaccactg 720tccggacaat
tcggacgaat cgcccaattg caacgcgtcc gccaaggcgt gccggggcga
780tcacaagtgc tcgcacgttt gcataccgct gcccgccggc cccacgtgcc
tctgccccct 840cggctaccac acgctcgacg agaagaactg cgaggacatc
aacgaatgcg aaacatacgg 900tatatgcgat caaatgtgcc ggaacacgcc
gggctcgtac gagtgctact
gcgacaaggc 960gtaccatttg gcggatgaca agaaaacctg caaggccgat
ggcgaggtgg gcgtcatggt 1020gttcagttcg aaggatcaga tcagagcggt
ggcgttgaat ttgaccagtt acatcctcgt 1080cgctaagaac ttgaagaaag
tggtgggcgt cgatttcgac ggtcgcaggg tctactggac 1140ggagatatac
gggggctacg agaacatcgc ccggagcgcc gaggacgggt cgcaaaagga
1200ggttttagta acggccggta tagatctgcc ggaggatttg gccatcgact
ggttgacggg 1260taacatttat ttcaccgatg gcgagaagaa acacatagga
gtttgctcgc ccgacgggac 1320ccattgcgct gttttaatca ataaggacat
cgcgaaaccc agaggcatcg ttcttaacgt 1380cttggacggc gacatgtatt
ggacggattg ggaagaaccg tcgcgaatcg gttactcatt 1440aatggacgga
tcaaatgata agcccttcgt agaaaaagac attcattggc ccaacggttt
1500agcattggat catcctaata gcagactgta ctggacggat gctaagaaga
tgacgttgga 1560aagcatccgt ttagatggaa ctgatcgaag gataatatta
gaagatatcg taaagcatcc 1620gtacgccata gcggtattcg aagataaatt
gtactggtcg gattgggcga cgcgcaccat 1680ccagcagtgc gacaaattcg
acgggaagaa tcaccggacg atgatcgaag agaaggaact 1740catctacggt
atcagcatat accatccggc gcaacagtcg cgcagcgtaa atccctgcga
1800gacggccctg tgcagcgacc tgtgcttgct gaaaggtaca tcgtacagct
gcgcttgtcc 1860gcagaacaag gtcctacagc ccgacggaat gttttgtaaa
gaattgtcgc ctatagagag 1920tctcatagcc gctcatcggg acatgttggt
acacatcgag catccgattc tcggcaggca 1980tgttgttaca gctctaccgg
gcgccactgg agacattgaa agcttggcat tcgatagctt 2040caaaaacgta
ctgtacatca gcgatttcaa gactaagaga atcaccactt tgcacatgaa
2100gaccggtacc agcaaagtaa tggatatacc cgatttgggt agaatcaacg
ctatggattt 2160cgattccaaa agcaacaatc tttacatctg cgattcggtc
cgcaaagtgg tagaagtaat 2220cagtatcaac acgatggctc ggcgaatttt
aattcacgac acgttaggag agtacccgca 2280aagtatcgcg ttagttcccg
acgaaggggt gatgtttata agcttcggag gcgagggacg 2340cagccacatc
gatcgcttct tcatggacgg caccggccga acgcacgcca tcgacaccaa
2400actgacgggg cccgtcgtgc tcgcgtacga cgccgatttg cacagggtct
tcttcgccga 2460cgccgccaac ggcgtcatcg aatccaccag cgtcgacggc
gacgatcgca tccacttcag 2520aaccgtcgac acgcacccca ccagcctggt
ggtcctcaaa gacgacgtgt tctggatgaa 2580cgcccgctcg aagcagctct
actggacgtc gaagaaagtc ccctcgaact acgacaagaa 2640aatcacgctc
gccttccccg acgatccgga caaggtgcac ctggcgtcgg tgacgtcgcg
2700ccgcaaggag accaacctct gtcgcatcaa caacaacggt tgcagccact
tgtgcgtcca 2760atcgcagaaa tcgatcgtgt gccgatgtcc gatcggttgg
gaactgaaac aggacaatcg 2820cacgtgcgcc aagcgagtgg gttgcatcag
cgacgaattt ctgtgcgcgc agtccaacgc 2880gtgcatcgtg aagagcctgc
gttgcgacgg ccggaaggat tgcctcttcg gcgaagacga 2940ggacgattgc
gcggcgacga agacgtgcgg cgacgggcag ttccgatgcg ccgacggcga
3000ttgcatcgcc gaagggctgg cgtgcaacct gcgctacgat tgcaaggata
aatcggacga 3060gcacggttgc ggcgacgtga tgaacggcac gcgatgcgcg
cccgatcact tcgcctgctc 3120gaacggcgaa tgcatcagcg ggcatttcca
ctgcgacggc gtctccgatt gcacggacaa 3180tacggacgag ttgcactgtc
agacgaagga gtgcaatgta acgcagttta ggtgcgattc 3240gggcgcttgt
attcccaagg agtgggagtg cgatcaggat tacgattgcg cggataattc
3300cgatgaacat tgcgatacgg tatgcccggc gagccacttc aaatgcgaca
acggcctgtg 3360cgtggataag aaattattgt gcgacggttt cgacaattgc
ggcgaccatt ccgacgagaa 3420gattcacatg tgtcaccaga gggccccgca
caattgcacc gcctacgaga cggcctgctc 3480gtccaacgct tccatttgca
ttcctttggg agcgaaatgc gacggcaaat ccgactgtcc 3540caagcacgag
gacgagatcg gctgcgccat gtgcgccgac gaggatttcg aatgccgcag
3600caaacacctc aaggactgca ttccgagatt gtggttgtgc gacggcgaga
acgattgcgg 3660cgacaacagc gacgaggact tggtgatgtg cagtaaaaag
aacaagacca cgttcgctcc 3720ggcccaacaa ccctgcaccg acggcttcag
atgcgacaac ggcgtttgca tcaactctac 3780gttgctctgt aacggcaaac
acgactgcta cgacggctcc gacgaaggag gctcctgctc 3840cgggtcctgc
gacggcccca agaatccctg caaccacgtc tgcgtcaaga cgccgaaggg
3900accgaagtgc cggtgcagac cgggctaccg gttgatgggc gacggtaaaa
cgtgcgtcga 3960cgtgaacgag tgcgaggccg atccgccgat atgcagccaa
ctttgccgca acaaagatgg 4020cggttacacg tgcgattgtt tccaaaactt
ttatttgagg aagaacatga aatcgtgcaa 4080agcggccggt tccgacatgt
tgatctactt caacgtgaac ggtgacgaaa tctacgaaat 4140ctcgccgaag
aacaattcga tgagcgtcgt ccaggacggg tcgatgttga agatctcgag
4200tctggccgcc gtggtggact cgaacgagct gttctacagc gtacccgaca
ccggcgccgt 4260ctacaagctc gatccggcca ccaaaaccgt tcgctacatc
gagaacttgg gcgagcccaa 4320actgatagcg gtggattggg cgacgcgtaa
tgtttattat tacgacgccg aatcggacgc 4380gaaatcgatt ggagtgtgca
atttcgagga gaagtgcgtc aaattgatcg acgtcgatgc 4440tcatcggcag
gtgtccgcct tgaccgtgga ttcataaatt gatcgacgtc gatgctcatc
4500gacaggtgtc cgccttggcc gtggattcgt acaacaaggt tttgttctat
gtgctgagat 4560cgtggacgat attcgcatcg cccagttacg ttttgtacaa
aaccgatttg gacggtagca 4620acgtggtgga actgatcaaa acaaatatag
gtaacgtgga acacatcacc tacgatttga 4680acaaacgcca gttgtacttc
cacgacgaaa acacccatca aatcaacacg atcggctaca 4740acgggggcaa
cgtcaaaccg ctcttctaca atgtatcatc actgatcgaa gggctcaaac
4800tgttcgaaga caatttgtac tatctgcgcc aggacgggtt cctcattagg
tgtcagttgt 4860tcgaagaggc cgtctgtcac tacggtttca atttgcacag
tctggccagg gacgatttcg 4920tcatctcgca ccgatccctt cagcccttgg
tcgaaaacgt ctgcgacggt cacagttgcc 4980cttacatgtg cgtggccgga
cgagagggct acaagtgcat ttgccacgat ggctcgacta 5040ctggcgaatg
cggtacacca caagacgaca acggtgcgaa caaattcaaa gtaaccacca
5100ttgaaggaaa atccaattct tcggcgttct tgtcgggctt cggggtggta
ctcgtgttac 5160tcgcttgcgt catgtttata gttgggttgt attacttgct
gaagaagaga agcgcagaaa 5220acagtacagc agatttaagt attgtccgtt
ttcaaaatcc gctttacgga agaccggtgg 5280aagacgagaa accgatcttg
gaacccggca agcacgagta cgtgaattgt ttgtacgaac 5340acaaaacgga
tggcggcgaa aacagcaatg cggaacccaa aacgtttaac gtctgtgatg
5400ataatttgtg ttaatttatt gtttttttta tgttttacgt taattaagtg
cattatttga 5460ttgttaattc tatttataac acattattat gtatatacaa
aatttgttaa atatttaata 5520aatatagag 5529255226DNAHalyomorpha halys
25atgctgtcgc cacgatatag gttaaaattt tccaacgatt tgaagttttt gtttgcgact
60atattaatcg ctttggtcac ttctactgaa cagcatgata cagattgtga tgaaaatttc
120ttcagatgcc gcaatggtag atgtatatct ctcagcttta tttgcgatgg
ttacgaagac 180tgcggccatg gggacatgtc tgatgaagaa aattgccatt
tgaccactac cccagcagtg 240aaaccgtgca ataaagatga atatcagtgc
aaagataggc tgtgcattcc ttcgttatgg 300gtctgcgatg gtgaatcaga
ctgttcggat ggcagcgatg agactcatgg atgtagtgag 360gcgacctgtg
acggttttca gtgccacagc aaatcttgta tccccaaaga gtgggagtgc
420gatggcgtac gtgattgtcc agataactca gacgaacagt tctgtgctaa
gaaaccagtg 480agcactgaag aatgcaatat tcatgaaaat ggtttcatgt
gccatgatag tatgaaatgc 540attgaactgg aagaagtctg caacagtcac
cctgattgcg atgatggttc tgatgaaggt 600ggtatgtgcc tcaatccgaa
caaaacagat ttatgctcta atctgaaatg tcccacaaat 660caaggctgta
taatattacc cgagggcccg gtatgtactg aggtgtgcaa gaaaggatac
720cacttcgcca gtggttcttg ttttgatatc gatgaatgta taacatttgg
aaggtgtgat 780caaatatgta taaacacaaa tggaggctac aattgctcct
gcattgaagg ctatagattt 840gataatgatt tgaaaaaatg caaagctaga
ggaagtgaag gtatactatt tttcacttca 900aatcaagaaa taagaggata
ttatttagac tccgaaatct actttaaagt agttgaaaat 960ctaccacatg
caacaggagt agcatatgat ggtgttaatg tttactggac aacagtagca
1020gatgaggaag aaacaattgt caaagcagct gaaaatggtg aaaatcagaa
aataattgta 1080acttctggta ttggcagtcc tgaagatcta gcagttgatt
gggttacaaa aaatgtatac 1140ttcacggatt ctaaattaaa gtatgtaggt
gtttgttcca atgatggata ctattgcctt 1200atactccatg aagatcaagt
tgagaaacca cgtgctattg ttctgcattc atcagaggga 1260ctgatgtatt
ggtctgactg gggaacagaa cctgccatta tgcgttctgg aatggatgga
1320tctaatgtta tcaaattcat aaaagataac attcattggc caaatggatt
agccattgac 1380tatgggacaa attatatata ttgggttgat gctaaagaag
caaaaattga atgtgtgaaa 1440ctagatggaa ctgacagaag aaaagtacca
tccagtgctg tgaaacatcc cttttctatt 1500gatatttttg aagatagaat
ctactggagt gactgggaca gtgaccaaat tgtatcctgc 1560aataaatttt
ctggtaaaga ttgccataca ataataaagg aaagagatca caagatttat
1620ggtatccatg tataccatcc tagcatgata aaagaagtta ttaatccatg
ctttgatgtt 1680caatgctctg atatatgctt aatagctcca aaaaatgcag
ataatgagtt ggaatattct 1740tgtgcttgcc catatcacaa aaaacttagc
agagatcatc atacatgtat tgaggataaa 1800ccacttcaaa ctataattgt
gggttctgga cagcagatct tcaaaatcca acacaaaaaa 1860tttggaggat
ttgtttcaga ccattttaaa ctaacaatat taaggaaaat aggtgctcta
1920gcttatgatc caaattcaga taaaatcatt gttagtgatt tggttagacg
aaaaattttt 1980tcagtcgact ggaaaactct agagtcagag ccattaattg
aacacaacat tggtagagtt 2040gttggtatgg atgttgacta ttatgaaaat
aatctatatt ggatcgatga tgagaaaaaa 2100actattgaag ttatgaacct
acaaaacaaa caccgattaa cactaatacg agatctaaat 2160gaaggtctta
atgatatagc attagtgtta gaacatgggt ttatgtttgt cgctctttca
2220acatttgaag gagcacacat tgatagaata tcaatggatg ggcgacatac
ttcaaggatt 2280catgttattg aagacaaatt atttggccca ttgtctctca
gctatgatcc taaacttgag 2340cgtctgttct ggtctgacca aatgggaggt
gaaatagctt ccacaggtgt tgatgggatt 2400gatcgacata tttttaaaga
aggacattct ggtccagtag atgtggcaat tactgaatcc 2460gaagtgtttt
ggcttggcta tggagccaga aaagtctttt ggtcaaataa gtttgatgga
2520tcgcttacaa aaagatttct tttagactca cttgatgaaa ctgataatat
gaaaatcatt 2580ggattaaata aaggtttaag aaaggaagca agtgcttgta
attccctcgg aaactgcagc 2640catgtttgtt tgctttcaac acctatgggg
atttgtgcat gtcctgatgg aatggagctt 2700ggatttgata gaaaaacctg
ccaaaaattg cttgcatgta aagaaggaca atacaaatgt 2760accactggag
aatgtatttc aaaatccatg cgatgtaatg gtagaccaga ttgtaggctt
2820ggtgatgatg aagagtattg ttctataatt tgttcaacaa acaagttcgc
ttgcatagat 2880ggttctagtt gtatagaaca atctcaaaaa tgtgattcta
aagttgactg caatgatggt 2940tctgatgaaa aatattgtga atcaaatcga
aattgtactg gaaatgaatt ccaatgtacg 3000tcaggagaat gtgttcctaa
agtggcacag tgtgatggca tgagtgattg cagtgatggt 3060agtgatgaaa
gcagttgtaa tactttcact tgcaaaacca attacttccg ttgcaacaca
3120ggaaattgta taccaagctc atgggaatgt gacagtcaga ttgactgtag
cgacggttct 3180gatgaaggtc ctaaatgttc tgagattgaa ggatgtggtg
aggatcagtt tacttgtgat 3240aatggtcact gcatttcaca tgttttggtg
tgtaaccatg aagatgattg tgaagactca 3300tctgatgaaa aatattgtta
ctttccaaaa accaaaaaaa attatgaaaa tgaagaaaat 3360aagactaagg
gcttatcaaa ggattgccag tttttatgtc cttcagattt aaaaatatgt
3420ttgcctgcat cagggagatg taatggtact tcagaatgcc cagacggaga
agatgaactt 3480cgttgcaaca agtgttcaga ggaagaattc acttgttctt
ctagtaagcg atgtttgcca 3540atgacatggg tttgtgacaa tgtcagtgac
tgtgaagacc agtccgatga aatgcattgt 3600cttaatggaa ccctggctgt
acatgatttg tataatcttc agtgtgatgg tttattctgc 3660aaagataata
aaaaatgcat tccattaaat aaactgtgtg attctcatat ggattgtaat
3720gatgggtctg atgaaaaagg tctttgtggt aagggatgtg aacatgctgc
atgtcatcac 3780aaatgtaaag agacacccat gggacctagg tgtgaatgtt
taagtggttt tactctagaa 3840ggcgatggga gggcatgtaa tgatataaat
gaatgtgaaa aactgtcttc ttgctctcaa 3900tattgccata attctgttgg
cagttacagt tgttcatgca tggattataa ctttcaacta 3960agatctgata
agattaggtg caaagcaaaa ggaactgaaa tgaagtatta ctttgccaca
4020aaaaaagaaa taaaatataa atcacaatcc ttacagcaaa ctgttgtagt
atatcaatca 4080cagcgtgata tggatatgaa aggaatggat tttgatatga
atagtgatgt aatattttgg 4140acttcagaga ttagtggtta tttgtacaaa
gttgatatta aatctaaaaa tgtcactcaa 4200attacaaatc tcattagacc
aactaaagta tcatatgact gggttactgg taatatttat 4260gtccttgaaa
acttcaaaat tattagagtt tgtaacttta aagctaaact ttgttcagca
4320atatacacag ctaagaatgg tataaacata gaaactattg ttcttgatcc
aaaatcaaga 4380atcatgttct ggtctgaaag taaatggtta atgagccaaa
caccaaatag tacacttcaa 4440caggcctcaa tgagtgcaga aaatccaagt
gtagtgctaa gttttgattt tagtccagtt 4500aatgatattg tagttgatca
ttttcacaag gttgtttact ggtcaaaccc cattgataat 4560aaaatagaaa
gatgtacata tgatggaaaa gacagaacta tagtagtgcg tagtgagtat
4620cctccaagag acttggtact atttgaagat tatttgtact gggtaaatga
tcaaaaacaa 4680tctcacttta ttcgcccaga agcagaagga actattacta
agtatggcct gtatggatca 4740gtgtacatga aacatgaatc agtaaggctt
ttaccttctt catcctataa tgaaattaaa 4800gctttccaag tttcacagtc
tgcactccag cctgaaggaa taaaccactg cagtaaaatt 4860acttgtaatt
acctgtgtat aagcaatgaa agagaaccag tatgtttctg tgaagatggc
4920ttaaaagtga aacctggtga tatatgctac aatcatactg ggatacattt
caaagcttac 4980gaaacagaaa atgtagcaga gagtagttca gtattgacat
ggtttttctg gattgttggg 5040ttagttctag catctggact tggttatgga
agttattatt ttgtagctaa ctacgttcag 5100aaaaataaaa tacacccacg
gttcagtgta ttttttcctg ttggccgttc tgagactcat 5160agagaacaag
gatctcaggt tcatcaattt gaaaatccta cgtatagtga ggtcattgtg 5220gtataa
5226265718DNAHalyomorpha halys 26gctgaacctc tgtaccttgg atcttcagtt
cccatttcat tttaccaagg cttccagtta 60gcttcgaact atgctgtcgc cacgatatag
gttaaaattt tccaacgatt tgaagttttt 120gtttgcgact atattaatcg
ctttggtcac ttctactgaa cagcatgata cagattgtga 180tgaaaatttc
ttcagatgcc gcaatggtag atgtatatct ctcagcttta tttgcgatgg
240ttacgaagac tgcggccatg gggacatgtc tgatgaagaa aattgccatt
tgaccactac 300cccagcagtg aaaccgtgca ataaagatga atatcagtgc
aaagataggc tgtgcattcc 360ttcgttatgg gtctgcgatg gtgaatcaga
ctgttcggat ggcagcgatg agactcatgg 420atgtagtgag gcgacctgtg
acggttttca gtgccacagc aaatcttgta tccccaaaga 480gtgggagtgc
gatggcgtac gtgattgtcc agataactca gacgaacagt tctgtgctaa
540gaaaccagtg agcactgaag aatgcaatat tcatgaaaat ggtttcatgt
gccatgatag 600tatgaaatgc attgaactgg aagaagtctg caacagtcac
cctgattgcg atgatggttc 660tgatgaaggt ggtatgtgcc tcaatccgaa
caaaacagat ttatgctcta atctgaaatg 720tcccacaaat caaggctgta
taatattacc cgagggcccg gtatgtactg aggtgtgcaa 780gaaaggatac
cacttcgcca gtggttcttg ttttgatatc gatgaatgta taacatttgg
840aaggtgtgat caaatatgta taaacacaaa tggaggctac aattgctcct
gcattgaagg 900ctatagattt gataatgatt tgaaaaaatg caaagctaga
ggaagtgaag gtatactatt 960tttcacttca aatcaagaaa taagaggata
ttatttagac tccgaaatct actttaaagt 1020agttgaaaat ctaccacatg
caacaggagt agcatatgat ggtgttaatg tttactggac 1080aacagtagca
gatgaggaag aaacaattgt caaagcagct gaaaatggtg aaaatcagaa
1140aataattgta acttctggta ttggcagtcc tgaagatcta gcagttgatt
gggttacaaa 1200aaatgtatac ttcacggatt ctaaattaaa gtatgtaggt
gtttgttcca atgatggata 1260ctattgcctt atactccatg aagatcaagt
tgagaaacca cgtgctattg ttctgcattc 1320atcagaggga ctgatgtatt
ggtctgactg gggaacagaa cctgccatta tgcgttctgg 1380aatggatgga
tctaatgtta tcaaattcat aaaagataac attcattggc caaatggatt
1440agccattgac tatgggacaa attatatata ttgggttgat gctaaagaag
caaaaattga 1500atgtgtgaaa ctagatggaa ctgacagaag aaaagtacca
tccagtgctg tgaaacatcc 1560cttttctatt gatatttttg aagatagaat
ctactggagt gactgggaca gtgaccaaat 1620tgtatcctgc aataaatttt
ctggtaaaga ttgccataca ataataaagg aaagagatca 1680caagatttat
ggtatccatg tataccatcc tagcatgata aaagaagtta ttaatccatg
1740ctttgatgtt caatgctctg atatatgctt aatagctcca aaaaatgcag
ataatgagtt 1800ggaatattct tgtgcttgcc catatcacaa aaaacttagc
agagatcatc atacatgtat 1860tgaggataaa ccacttcaaa ctataattgt
gggttctgga cagcagatct tcaaaatcca 1920acacaaaaaa tttggaggat
ttgtttcaga ccattttaaa ctaacaatat taaggaaaat 1980aggtgctcta
gcttatgatc caaattcaga taaaatcatt gttagtgatt tggttagacg
2040aaaaattttt tcagtcgact ggaaaactct agagtcagag ccattaattg
aacacaacat 2100tggtagagtt gttggtatgg atgttgacta ttatgaaaat
aatctatatt ggatcgatga 2160tgagaaaaaa actattgaag ttatgaacct
acaaaacaaa caccgattaa cactaatacg 2220agatctaaat gaaggtctta
atgatatagc attagtgtta gaacatgggt ttatgtttgt 2280cgctctttca
acatttgaag gagcacacat tgatagaata tcaatggatg ggcgacatac
2340ttcaaggatt catgttattg aagacaaatt atttggccca ttgtctctca
gctatgatcc 2400taaacttgag cgtctgttct ggtctgacca aatgggaggt
gaaatagctt ccacaggtgt 2460tgatgggatt gatcgacata tttttaaaga
aggacattct ggtccagtag atgtggcaat 2520tactgaatcc gaagtgtttt
ggcttggcta tggagccaga aaagtctttt ggtcaaataa 2580gtttgatgga
tcgcttacaa aaagatttct tttagactca cttgatgaaa ctgataatat
2640gaaaatcatt ggattaaata aaggtttaag aaaggaagca agtgcttgta
attccctcgg 2700aaactgcagc catgtttgtt tgctttcaac acctatgggg
atttgtgcat gtcctgatgg 2760aatggagctt ggatttgata gaaaaacctg
ccaaaaattg cttgcatgta aagaaggaca 2820atacaaatgt accactggag
aatgtatttc aaaatccatg cgatgtaatg gtagaccaga 2880ttgtaggctt
ggtgatgatg aagagtattg ttctataatt tgttcaacaa acaagttcgc
2940ttgcatagat ggttctagtt gtatagaaca atctcaaaaa tgtgattcta
aagttgactg 3000caatgatggt tctgatgaaa aatattgtga atcaaatcga
aattgtactg gaaatgaatt 3060ccaatgtacg tcaggagaat gtgttcctaa
agtggcacag tgtgatggca tgagtgattg 3120cagtgatggt agtgatgaaa
gcagttgtaa tactttcact tgcaaaacca attacttccg 3180ttgcaacaca
ggaaattgta taccaagctc atgggaatgt gacagtcaga ttgactgtag
3240cgacggttct gatgaaggtc ctaaatgttc tgagattgaa ggatgtggtg
aggatcagtt 3300tacttgtgat aatggtcact gcatttcaca tgttttggtg
tgtaaccatg aagatgattg 3360tgaagactca tctgatgaaa aatattgtta
ctttccaaaa accaaaaaaa attatgaaaa 3420tgaagaaaat aagactaagg
gcttatcaaa ggattgccag tttttatgtc cttcagattt 3480aaaaatatgt
ttgcctgcat cagggagatg taatggtact tcagaatgcc cagacggaga
3540agatgaactt cgttgcaaca agtgttcaga ggaagaattc acttgttctt
ctagtaagcg 3600atgtttgcca atgacatggg tttgtgacaa tgtcagtgac
tgtgaagacc agtccgatga 3660aatgcattgt cttaatggaa ccctggctgt
acatgatttg tataatcttc agtgtgatgg 3720tttattctgc aaagataata
aaaaatgcat tccattaaat aaactgtgtg attctcatat 3780ggattgtaat
gatgggtctg atgaaaaagg tctttgtggt aagggatgtg aacatgctgc
3840atgtcatcac aaatgtaaag agacacccat gggacctagg tgtgaatgtt
taagtggttt 3900tactctagaa ggcgatggga gggcatgtaa tgatataaat
gaatgtgaaa aactgtcttc 3960ttgctctcaa tattgccata attctgttgg
cagttacagt tgttcatgca tggattataa 4020ctttcaacta agatctgata
agattaggtg caaagcaaaa ggaactgaaa tgaagtatta 4080ctttgccaca
aaaaaagaaa taaaatataa atcacaatcc ttacagcaaa ctgttgtagt
4140atatcaatca cagcgtgata tggatatgaa aggaatggat tttgatatga
atagtgatgt 4200aatattttgg acttcagaga ttagtggtta tttgtacaaa
gttgatatta aatctaaaaa 4260tgtcactcaa attacaaatc tcattagacc
aactaaagta tcatatgact gggttactgg 4320taatatttat gtccttgaaa
acttcaaaat tattagagtt tgtaacttta aagctaaact 4380ttgttcagca
atatacacag ctaagaatgg tataaacata gaaactattg ttcttgatcc
4440aaaatcaaga atcatgttct ggtctgaaag taaatggtta atgagccaaa
caccaaatag 4500tacacttcaa caggcctcaa tgagtgcaga aaatccaagt
gtagtgctaa gttttgattt 4560tagtccagtt aatgatattg tagttgatca
ttttcacaag gttgtttact ggtcaaaccc 4620cattgataat aaaatagaaa
gatgtacata tgatggaaaa gacagaacta tagtagtgcg 4680tagtgagtat
cctccaagag acttggtact atttgaagat tatttgtact gggtaaatga
4740tcaaaaacaa tctcacttta ttcgcccaga agcagaagga actattacta
agtatggcct 4800gtatggatca gtgtacatga aacatgaatc agtaaggctt
ttaccttctt catcctataa 4860tgaaattaaa gctttccaag tttcacagtc
tgcactccag cctgaaggaa taaaccactg 4920cagtaaaatt acttgtaatt
acctgtgtat aagcaatgaa agagaaccag tatgtttctg 4980tgaagatggc
ttaaaagtga aacctggtga tatatgctac aatcatactg ggatacattt
5040caaagcttac gaaacagaaa atgtagcaga gagtagttca gtattgacat
ggtttttctg
5100gattgttggg ttagttctag catctggact tggttatgga agttattatt
ttgtagctaa 5160ctacgttcag aaaaataaaa tacacccacg gttcagtgta
ttttttcctg ttggccgttc 5220tgagactcat agagaacaag gatctcaggt
tcatcaattt gaaaatccta cgtatagtga 5280ggtcattgtg gtataataca
atataaatat gatgtttatt taagaaaaaa caaatatatg 5340catatatata
tgtatacact tatatataca catatatatg tatatattta tagatacaca
5400cattaaatta agttgtttat ctttttctat aggagaactc tgaatttaaa
tctttgttaa 5460acaatgaaat atctgtgaat caagtgtaga aataattgat
ttacattgta aacctccgtg 5520taacaagttt cttattaaca ttatattatt
accatatcat ataaatatga atttcctagt 5580aactgattgt tcttattact
atttttgact gattaaagta atgtactctc agtttaaatc 5640agcaaagagc
tatttaagct ctataaaact gtattattat caaatattta atatttgaac
5700tgtaatttat tgttttaa 5718275307DNAAcyrthosiphon pisum
27atggacaatg gcgctgacga agacgattgt gatattagca aaaagccttg cctggcgcca
60gaatactttg aatgtgataa caagagaaaa tgcattccta aggaatttgt gtgtgataag
120tcgaatgaat gcatcgacaa gtctgacgaa agtcattgta aagagacgtc
atcatcttta 180acgcagtcat gccgggaagg aaaattcaaa tgctccgatg
gcacttgtat tccaatggct 240tggcattgtg acgcggagat tgattgccac
gacggtagcg atgaagacgg agaaaaatgt 300agatacacga cattgtgtac
ctctggttat atgtgcaaaa acttcaattg cgtttcaaag 360acgtggagat
gtgacggaaa agatgactgt ggagatggtt cagacgaaac ccattgcgat
420atccaactgg ttgagccaga aaaatgttta attgaaaatc gtaaatttct
atgtcacgat 480cataaaaaat gtatcgatgt cagaaatgtc tgtgattaca
acaatgactg tcttgatgca 540tcggacgaag gtggtctttg caataataaa
actgatttca aaagtgaatg taattcgatg 600aattgttctg ccgcatttga
atgtttaaga aagccacatg gcattatgtg tgtgtgccca 660aaaggaatgc
attatttaaa taataaatgt tctgatatca acgaatgtga acaatatggt
720atttgtgacc aagtgtgtct taatttggaa ggttcatata cgtgctcctg
tgatccacat 780tatgaactag tagataatca taagtgtaaa ataaaaggcc
taaatccgga attattatat 840tcatcactac gtcaaataaa agtttttaac
ttggaattac ttcatagttt tactttgatt 900gatgatttgc aacatgtcac
tggattagct gttgataaat taactttata ctggacactt 960tatttggatg
ggaactcagc tattgttaga gcaaacaagt ctgagcctaa accagaaatt
1020attgttgact cgggtcttgg atcgcctgag aatctagtta tagatattat
tacacataat 1080ttgtacttca ccgatgcaaa aatgaaacat attggagtgt
gcaataatga tggttcagta 1140tgtacagttc ttcataataa aaacatagac
aaaccaagag cagtagctgt ttcaccacta 1200gatggtctca tgtactggac
agattggggt aaaaatccaa tgattggtcg ttctggtatg 1260gacgggtcaa
gaccacaacc gtttgttaca caaaatatac actggccaaa tggtgttcat
1320gttgattatg tcgggaaaag aatttactgg gtggatgcta agatgcaatt
tattgaatcc 1380attaatttag atgcaactga tcgaagagta attgtaacag
aatatgtaga tcatccatat 1440tcggttactg tgttcgagga taaattgtac
tggagtgatt ggtcaagtaa agaaataaaa 1500gtatgcaaca aatttacggg
taaagatagc aagacattga tacgtgaaaa taagaacaga 1560gtgtatggca
tgcaaattaa ccatccctcg ctcttcgagt ctcaaatgcc aaatccttgt
1620gaacaaggaa aatgcagtga tatatgctta ttagcaccga aaacagaaat
caattctaaa 1680ggatactcgt gcgcttgccc cgatgataaa aaactatcgg
aagatggaat cttttgtatc 1740accattgcca tacctcctac gcttattgtg
ggtacaccaa caagtattat agaaattgag 1800caggaacatt taggccgcca
aaaagcaaaa aaaatatcgt tgaaaaatga tatatctagc 1860atatccgcat
tgacttataa ttcattatct ggtgacatca tcatctatga ctcaaggtct
1920aagaaattat tcactttcga ttttgttaag atgaaattat cagatttaga
tgtaccagaa 1980attagttcca tttattctct tgaattcgac aatcatggaa
acaatttata ctggtgtgat 2040aggactagaa aaacattaga tgtgttaagt
ctctctacaa aaacacacac aacgatattc 2100aaagaaattg aaggtcatat
cccatttgcc gttactttag ttccagataa aagctttatg 2160tttgtagcta
tgaaagaagg ttcacacata catatcgatc gaattgacat ggatggaagt
2220attcagtctc tagtacatat ggtggagtat ggattgacag gagatgaaat
tgctttacat 2280tttgatatga ttacaagact gttgtatttt actgactata
aaaatggcat tattgatagt 2340gtcagcgaag atggaacgga tagaagaatt
ataaaaaaag caggatttgt aagagatatt 2400gtttcaattg gttatgacat
tgtttgggta tctgaaggtt cgaaaatttt aaactggatt 2460gataacgttg
atgaagaccg agcatctaga gctcttgata tagataattg gaaaactgat
2520tcaaatttat atttaaccgt agcaaatgga attcacaaaa atatacaaca
cccgtgtcaa 2580caaaataatg gtggttgtag tcatatatgc cttttatctg
agaaaggaaa ggtttgtggt 2640tgtccacctg gattagtttt atcaaacaac
agcctgaatt gtacatcact aggagagtgt 2700aaatctgatg aatatcgttg
ttacactggt gaatgtattt catctcattc ccgatgtgat 2760tttataaaag
attgtcctcg tggtgacgat gaagacgttg ttaaatgttt gcattacaca
2820ccaaactcat gtccacaatc tcaattttta tgtcatgata aaacaaagtg
cttggataaa 2880aaacatatgt gtgatagtat taaagactgt agtgatggtt
cagatgaaat ggattgttta 2940cacaagcaca cttgtgattc aactacagaa
tatcagtgta catctggaga gtgtattcag 3000cgcatatttt tatgtgatgg
taatcccgac tgtattaatg gacaagacga gttaaattgt 3060ctgaaccaaa
gttgtaataa tattacagaa tttagatgta attctggaaa ctgtatacct
3120gcaacatggg aatgcgatgg tgaggttgat tgttttgatg gttcggatga
acactattca 3180tgtgctacca aaaaatgtaa agatgatcag ttttcttgca
ccaatggtcg atgtatatca 3240cataaattca cttgtaacgg taaagatgat
tgtggtgata gttctgatga aaatggttgt 3300tcgtcaaatc atgcatacat
gacaaaaaga gtttcaaaag tatgtaatga aaaaacagaa 3360tttgaatgtg
aaaacacaat gggacattgt ataccgatca aggctaggtg caacggcaca
3420tccgagtgca aacatttaga ggacgaattg aattgtggat gtcaaaaatc
taattttttc 3480gaatgtcaga ataagcgttg tgtgttaaaa gattggttat
gtgataaaca tgatgattgt 3540ggcgatgggt cagatgaaag tcaaaaggcg
tgtgatatgc tttctgaaca tttatcgaat 3600tcagtcgttt catcaaaaga
ctgtgatgga tatttatgta agaaccaaga atgtatacct 3660ctagatcaag
cttgtaataa gaaaatcaat tgtaaagatg gttcagacga aggagatctt
3720tgtggtttat cttgtataca catggattgt agtcatactt gtcaagaaac
tccaaaaggt 3780gggaaatgta catgttatca tggctataaa cttgcacaag
atggtataac ttgcagagat 3840attaatgaat gtgaagacga caatttatgt
actcaatatt gtacaaattc agatggttca 3900tatagctgta gttgtttaaa
ttctgattac attttgcgtg cagataaaag ttcctgtaaa 3960gcaattggac
ccacaatgga tttagtttat tcgagtgttg atgaaataag aagtacttca
4020ggagatatta aggaatcaaa attaattttt tcgatgcctg gaatgacaat
atcgagcctt 4080gatatagata ttagaagaaa tcttatttat tggacatcaa
aacaagctgg tgttcttatt 4140tgtatggata tgcaacaaca gcataaattt
tacatgacag atttgttaca cccaacttta 4200gttcgtattg attggttaac
agaaaacgtt tactttgtac aaaattttaa agatattgtt 4260gtatgttatt
taaatgctaa acgttgtgcc actatataca gtgcaaacat ccatacaaca
4320attatggctt ttgaaattga tcctcgttcc ggagtgatgt tctggtcgga
aaccatatgg 4380gcagtatttt caacaccaac aactattatt aggaaatctg
attgcagtgg atacaatgta 4440aaaactataa tccatcaagg gttacagtat
gttactgatt tagctataga tccaatcaaa 4500catatggtct attgggtcga
tcaagttaat tcaacaatcg agaggtctaa ttacgacgga 4560actaaaataa
ctgtgttggt caattcgata gaacatttgc cagagaaaat atcattgtac
4620gaagacaaac tgttttggac caaccatgaa accgggttgt ctatatttaa
atgtaaagtt 4680ctcggttctg aaaatgtcca ctgtgagtct gttcctgttc
aagtgtttgc taccatcgag 4740acattcacga taagtcaaca ggccaaacaa
agaaatggat caaatgtttg cattgatgtc 4800gattgcgatt ttatttgtac
ttctggatca aaaggaccga tgtgtgtttg tggagatgga 4860tctcaagtcg
agccaggcaa tatttgtgat gatgctggtc atagaaaatt gcctgttttt
4920aaagacttag attacaaaaa aaatatgatt tcaatctata tttctatatt
tttaattgta 4980atgttgttat tagcgatgtt atattatttt ctattctatt
ccaaaaataa atctttgaat 5040caactagtat ctagattatt tcatcggcca
ttaacaactg tcatccaagt aattgaccag 5100aaagaagatg aaataaattc
aagatataat gtttatgaga atcctatgaa cgaagatggg 5160ttaataaaag
accatgcttg tggaagtgaa atcgtcttga actatgatag tgcacgttac
5220gttaatgcta actgctcaga acctccactt aactcgtttt cagagtacga
cgagaacgag 5280aaaaaaaaac ttattatggt tttataa
5307285772DNAAcyrthosiphon pisum 28attgaatgtt ttttcagaaa aagaatgtac
taaatacgaa ttcaactgcg ggaacggtac 60ttgtatcaat aagacaagac tttgtgacgg
cattgttgac tgtatggaca atggcgctga 120cgaagacgat tgtgatatta
gcaaaaagcc ttgcctggcg ccagaatact ttgaatgtga 180taacaagaga
aaatgcattc ctaaggaatt tgtgtgtgat aagtcgaatg aatgcatcga
240caagtctgac gaaagtcatt gtaaagagac gtcatcatct ttaacgcagt
catgccggga 300aggaaaattc aaatgctccg atggcacttg tattccaatg
gcttggcatt gtgacgcgga 360gattgattgc cacgacggta gcgatgaaga
cggagaaaaa tgtagataca cgacattgtg 420tacctctggt tatatgtgca
aaaacttcaa ttgcgtttca aagacgtgga gatgtgacgg 480aaaagatgac
tgtggagatg gttcagacga aacccattgc gatatccaac tggttgagcc
540agaaaaatgt ttaattgaaa atcgtaaatt tctatgtcac gatcataaaa
aatgtatcga 600tgtcagaaat gtctgtgatt acaacaatga ctgtcttgat
gcatcggacg aaggtggtct 660ttgcaataat aaaactgatt tcaaaagtga
atgtaattcg atgaattgtt ctgccgcatt 720tgaatgttta agaaagccac
atggcattat gtgtgtgtgc ccaaaaggaa tgcattattt 780aaataataaa
tgttctgata tcaacgaatg tgaacaatat ggtatttgtg accaagtgtg
840tcttaatttg gaaggttcat atacgtgctc ctgtgatcca cattatgaac
tagtagataa 900tcataagtgt aaaataaaag gcctaaatcc ggaattatta
tattcatcac tacgtcaaat 960aaaagttttt aacttggaat tacttcatag
ttttactttg attgatgatt tgcaacatgt 1020cactggatta gctgttgata
aattaacttt atactggaca ctttatttgg atgggaactc 1080agctattgtt
agagcaaaca agtctgagcc taaaccagaa attattgttg actcgggtct
1140tggatcgcct gagaatctag ttatagatat tattacacat aatttgtact
tcaccgatgc 1200aaaaatgaaa catattggag tgtgcaataa tgatggttca
gtatgtacag ttcttcataa 1260taaaaacata gacaaaccaa gagcagtagc
tgtttcacca ctagatggtc tcatgtactg 1320gacagattgg ggtaaaaatc
caatgattgg tcgttctggt atggacgggt caagaccaca 1380accgtttgtt
acacaaaata tacactggcc aaatggtgtt catgttgatt atgtcgggaa
1440aagaatttac tgggtggatg ctaagatgca atttattgaa tccattaatt
tagatgcaac 1500tgatcgaaga gtaattgtaa cagaatatgt agatcatcca
tattcggtta ctgtgttcga 1560ggataaattg tactggagtg attggtcaag
taaagaaata aaagtatgca acaaatttac 1620gggtaaagat agcaagacat
tgatacgtga aaataagaac agagtgtatg gcatgcaaat 1680taaccatccc
tcgctcttcg agtctcaaat gccaaatcct tgtgaacaag gaaaatgcag
1740tgatatatgc ttattagcac cgaaaacaga aatcaattct aaaggatact
cgtgcgcttg 1800ccccgatgat aaaaaactat cggaagatgg aatcttttgt
atcaccattg ccatacctcc 1860tacgcttatt gtgggtacac caacaagtat
tatagaaatt gagcaggaac atttaggccg 1920ccaaaaagca aaaaaaatat
cgttgaaaaa tgatatatct agcatatccg cattgactta 1980taattcatta
tctggtgaca tcatcatcta tgactcaagg tctaagaaat tattcacttt
2040cgattttgtt aagatgaaat tatcagattt agatgtacca gaaattagtt
ccatttattc 2100tcttgaattc gacaatcatg gaaacaattt atactggtgt
gataggacta gaaaaacatt 2160agatgtgtta agtctctcta caaaaacaca
cacaacgata ttcaaagaaa ttgaaggtca 2220tatcccattt gccgttactt
tagttccaga taaaagcttt atgtttgtag ctatgaaaga 2280aggttcacac
atacatatcg atcgaattga catggatgga agtattcagt ctctagtaca
2340tatggtggag tatggattga caggagatga aattgcttta cattttgata
tgattacaag 2400actgttgtat tttactgact ataaaaatgg cattattgat
agtgtcagcg aagatggaac 2460ggatagaaga attataaaaa aagcaggatt
tgtaagagat attgtttcaa ttggttatga 2520cattgtttgg gtatctgaag
gttcgaaaat tttaaactgg attgataacg ttgatgaaga 2580ccgagcatct
agagctcttg atatagataa ttggaaaact gattcaaatt tatatttaac
2640cgtagcaaat ggaattcaca aaaatataca acacccgtgt caacaaaata
atggtggttg 2700tagtcatata tgccttttat ctgagaaagg aaaggtttgt
ggttgtccac ctggattagt 2760tttatcaaac aacagcctga attgtacatc
actaggagag tgtaaatctg atgaatatcg 2820ttgttacact ggtgaatgta
tttcatctca ttcccgatgt gattttataa aagattgtcc 2880tcgtggtgac
gatgaagacg ttgttaaatg tttgcattac acaccaaact catgtccaca
2940atctcaattt ttatgtcatg ataaaacaaa gtgcttggat aaaaaacata
tgtgtgatag 3000tattaaagac tgtagtgatg gttcagatga aatggattgt
ttacacaagc acacttgtga 3060ttcaactaca gaatatcagt gtacatctgg
agagtgtatt cagcgcatat ttttatgtga 3120tggtaatccc gactgtatta
atggacaaga cgagttaaat tgtctgaacc aaagttgtaa 3180taatattaca
gaatttagat gtaattctgg aaactgtata cctgcaacat gggaatgcga
3240tggtgaggtt gattgttttg atggttcgga tgaacactat tcatgtgcta
ccaaaaaatg 3300taaagatgat cagttttctt gcaccaatgg tcgatgtata
tcacataaat tcacttgtaa 3360cggtaaagat gattgtggtg atagttctga
tgaaaatggt tgttcgtcaa atcatgcata 3420catgacaaaa agagtttcaa
aagtatgtaa tgaaaaaaca gaatttgaat gtgaaaacac 3480aatgggacat
tgtataccga tcaaggctag gtgcaacggc acatccgagt gcaaacattt
3540agaggacgaa ttgaattgtg gatgtcaaaa atctaatttt ttcgaatgtc
agaataagcg 3600ttgtgtgtta aaagattggt tatgtgataa acatgatgat
tgtggcgatg ggtcagatga 3660aagtcaaaag gcgtgtgata tgctttctga
acatttatcg aattcagtcg tttcatcaaa 3720agactgtgat ggatatttat
gtaagaacca agaatgtata cctctagatc aagcttgtaa 3780taagaaaatc
aattgtaaag atggttcaga cgaaggagat ctttgtggtt tatcttgtat
3840acacatggat tgtagtcata cttgtcaaga aactccaaaa ggtgggaaat
gtacatgtta 3900tcatggctat aaacttgcac aagatggtat aacttgcaga
gatattaatg aatgtgaaga 3960cgacaattta tgtactcaat attgtacaaa
ttcagatggt tcatatagct gtagttgttt 4020aaattctgat tacattttgc
gtgcagataa aagttcctgt aaagcaattg gacccacaat 4080ggatttagtt
tattcgagtg ttgatgaaat aagaagtact tcaggagata ttaaggaatc
4140aaaattaatt ttttcgatgc ctggaatgac aatatcgagc cttgatatag
atattagaag 4200aaatcttatt tattggacat caaaacaagc tggtgttctt
atttgtatgg atatgcaaca 4260acagcataaa ttttacatga cagatttgtt
acacccaact ttagttcgta ttgattggtt 4320aacagaaaac gtttactttg
tacaaaattt taaagatatt gttgtatgtt atttaaatgc 4380taaacgttgt
gccactatat acagtgcaaa catccataca acaattatgg cttttgaaat
4440tgatcctcgt tccggagtga tgttctggtc ggaaaccata tgggcagtat
tttcaacacc 4500aacaactatt attaggaaat ctgattgcag tggatacaat
gtaaaaacta taatccatca 4560agggttacag tatgttactg atttagctat
agatccaatc aaacatatgg tctattgggt 4620cgatcaagtt aattcaacaa
tcgagaggtc taattacgac ggaactaaaa taactgtgtt 4680ggtcaattcg
atagaacatt tgccagagaa aatatcattg tacgaagaca aactgttttg
4740gaccaaccat gaaaccgggt tgtctatatt taaatgtaaa gttctcggtt
ctgaaaatgt 4800ccactgtgag tctgttcctg ttcaagtgtt tgctaccatc
gagacattca cgataagtca 4860acaggccaaa caaagaaatg gatcaaatgt
ttgcattgat gtcgattgcg attttatttg 4920tacttctgga tcaaaaggac
cgatgtgtgt ttgtggagat ggatctcaag tcgagccagg 4980caatatttgt
gatgatgctg gtcatagaaa attgcctgtt tttaaagact tagattacaa
5040aaaaaatatg atttcaatct atatttctat atttttaatt gtaatgttgt
tattagcgat 5100gttatattat tttctattct attccaaaaa taaatctttg
aatcaactag tatctagatt 5160atttcatcgg ccattaacaa ctgtcatcca
agtaattgac cagaaagaag atgaaataaa 5220ttcaagatat aatgtttatg
agaatcctat gaacgaagat gggttaataa aagaccatgc 5280ttgtggaagt
gaaatcgtct tgaactatga tagtgcacgt tacgttaatg ctaactgctc
5340agaacctcca cttaactcgt tttcagagta cgacgagaac gagaaaaaaa
aacttattat 5400ggttttataa gcatcataaa attataaact ttgttaagga
tgaatattta tttgttaatt 5460tttattttta taaatacaat tttattttat
ttttataata tcaatatgca cattgcacat 5520agatataaat tattactgaa
atttataatt ctgtatacct aaatgattat tttttttagt 5580ttgttaactt
ttacaatagt tccttatttt taatttgttt aattcaaagt tacccggtat
5640atatatatta ttataataat aaataaaagt tacattaaat tagtgtgatt
gtgtcaatat 5700atcaaaagct aacacttaat ataattcaat gtatattcaa
aagatctgcg tgtgtaatga 5760aattaaatat at 5772295430DNABemisia tabaci
29atgattcaga gggaatggag ctccatatcc aagggctcat ggtgcacagc attattagta
60gttatcgcag tcttttgcac atttgtacaa tcatcttcat catatgaatg tgtcggacca
120tcccatttcg aatgtacaaa tcacaggtgt atatccatgg atttaaggtg
cgatggagat 180gacgattgta atgatggatc cgatgaacac ggttgtaatg
tggataaatc aaaaaatgaa 240acctgtgcta gtacccagtt tgattgcggt
cagggccaat gtataccacg gtcctgggtt 300tgtgatggga atgcagattg
tgaggacggt aaagatgaag gagctgtagg ctgcgctgaa 360agccattgcg
cagcgtctga gtgggagtgc cctcataacc atcgctgtat cccgaatgat
420tatatctgtg atggggatga tgattgtggt gacaattcag atgaagacga
ctgtacaggc 480aagaataatt ttacagagtg cacttcagcg tttggaaaat
ttctctgcaa aaataggaat 540cagtgcatcg atgattctct tctgtgcaac
gggcaccctg actgtaaaga tgggtcagat 600gagggtggtc actgtgcttc
gaaagctcaa gtagctactg actgtgcaaa gctaaactgt 660acccactcgt
gtgttgaaag cccagatgga ccggtttgcg tctgtggatc aggatatcac
720cttgaaggaa acgtctgtga agatattaat gagtgcttgg aatggggaac
atgtgaccaa 780atgtgtgaaa acacggtcgg aggttacatc tgtgaatgtg
aacctggata caaactggaa 840agtaacggac gcacatgcaa agcagaagag
ggtgaaggac ttctgatcta ttcaagcctc 900aaaaagatta agtctctata
tctgacatct cgcatatcaa tgacagttgc ctctgaggtt 960ccatatgcga
caggtgtgtc ttttgatggt aaacatgtct attggacgac cgtgcttgat
1020ggagttgaat ctattgttcg ggcaagtgag gatggatctc atgaaaccac
cattgttgat 1080tcaggtgttg gctcccctga agacctagct gtcgactggg
tgactggtaa catatacttc 1140actgatggcg agtaccaaca gatcggcatt
tgcacctaca acgaagagct ggttgaaacg 1200aaatgcgctg tcctccacaa
caaagacctg aacaagcccc gcgcaattgt tttaaaccca 1260gctgatgcgg
tcatgtactg gtctgattgg ggctttaaac cactcatcgc ccgttctgga
1320atggatggca cagacttcta tgagtttgta acgacagagc tccattggcc
caatggtctc 1380acaattgatc acggaaatcg gagagtatat tgggtcgatg
cgagactcgg aactgttgaa 1440actgttgatt ttcaaggccg tgatcggcgg
aaaatattaa ctgatctcaa tgatcatcct 1500ttcgcaatcg ctgtttttga
agataaaatc tattggagtg gatggacaaa ccaagaaata 1560gtagaatgta
ataagttcac cgggaaaaac cgagtacaag ttgtcaaaag tcggaaagac
1620aaaatttacg gtgtgcacat ttttcaccca actttgcaaa atcattcgct
accaaatccg 1680tgcgccggga agtgcagtga catctgtgct ctatctccat
cagcttcaag cggaggcaaa 1740ggctactctt gcttgtgccc tgacaataaa
atcttatccc cctctggaga atggtgccaa 1800gaacagccca aagaatctgt
cattgttagc attggaaact ttgttttcca gctgaaagtg 1860actcttggga
agcagtacat tcatccgctg cctgttaata acctccagtc tgtcagtgca
1920atcgtctaca actcctttga tggctctcta ttaatagctg acccagatgc
caaaatgatt 1980tactcgtatc aactgaacac ggacaccatg gagactctaa
ttgatctcaa agtgggctat 2040gtctcagcat tggcttacga tcccattggc
cggaatctct attggtgcga caaagaggcc 2100ggcactgtag aagtgttcag
tttcttctct cacagaagga agttgttgct ccgggaattt 2160gacgatgaaa
aacctttcgc catgactctc ataccagaag aaggcctgat gttcgtcatt
2220gccaaagccc atgaccacct tcacattgat cggatcaaca tggatggctc
actaagcaca 2280ctgactcaca tgaccagtct caagttgcaa ggaccggatg
tagctttgca ctatgacagt 2340gattctcgca gggtttattg ggctgatcac
tcagctggcc tcattgagag caccgatacg 2400aatggaaacg acaggcaagt
ataccgtgac gtatcatcac ctttagctct gaccgatgtg 2460gatagagacc
tttattggac atcagatggt cgcccacacc tgtactactc tgagaaaagc
2520aatgcaagca tgcccgtcag gaaaatcaac atggaacgat ttttgcgatc
accaaaggac 2580cactatcgga tgtttgttac tgcaatcatc cctgataaaa
ctactagaga tcatccttgc 2640caaactaaca atggcaagtg tagccatttt
tgcctcctca ccagtcgtaa tccaaagcat 2700gtatgcagtt gtcctgatgg
gatgaagctg gcggataatg gtcaggactg tgaagagatt 2760gctgcatgtg
gagcacatga ataccattgc acgacaggtg aatgcattcc aatgtcgaag
2820aaatgcgacc gaaacaagga ttgcccctat ggcgaagatg agaccttctg
cccagcacag 2880tgtgagactg atcagtttgc gtgctttgat ggacaaaaat
gtatcgatgc caaggataga 2940tgcaacatgc attttgattg tcacgaccac
tctgatgagg ctaactgcca aaatgtcacc 3000tgcgatcaat catacaactt
tttgtgccga atcggtgagt gcgtaagtca cgctgtgctg 3060tgcaataatg
aatggaattg caaggacggt agtgatgaag agaactgtac gacttccacg
3120tgcccttcca gcgagtttcg gtgtcattcc ggaacatgca
taccgaagaa ctgggtctgt 3180gatctggatg ccgattgccc cgaccaatca
gatgaaaata actgcagttt ctcaagaaaa 3240gaaaagtgca ccgaattctt
gtgtcagagt ggcatgtgtg tagcacagga acttgtctgt 3300aacggtcaaa
ccgaatgtga cgatggaagt gacgaattca attgcgatga gccggttcca
3360aaaacggcaa acaaagagga tggttttata gataactgtg atgaagagaa
ggagttcatg 3420tgcgagcctg gcaaatgcat taatcttata ttcaagtgta
atggtgttaa agattgcgag 3480aacggagctg atgagctaaa ctgcataggc
tgtgaacaat tcacttgcaa taatgggaaa 3540tgcatcactt atgatcttgt
atgcaatgat gatgatgact gtggtgactc atccgatgaa 3600agacctctca
actcgtgccc agacagcaaa gaaaacccag ctattgtgcc tgctcatatt
3660cccaatgtgt gccatggatt tgtttgcaag aacggtgaat gccttgatga
tttcagtcta 3720gtctgcaaca aaaagcaaga ttgtaaggac ggctcagatg
aaggtggacg atgcggttca 3780agctgtgatg tgacagcaaa ttgcagtcag
atttgtcgtg ataagccaaa tggacatgag 3840tgtgcgtgtg tccctggctt
taaaattgct gaggatggcc gcgattgcga ggatattgat 3900gagtgcaccg
aattggagcc gtgtagccag atgtgtttca acacctatgg aagttacacc
3960tgtgcctgtc ttggtccaga ttatattaaa aagtcagatg ggtcctgcaa
agccactggc 4020ccaaaattgc agtacgtctt tgctaccggt taccagattc
gcactatatc atacctaatg 4080accgatgtca aagtggcata ctatagtgca
gatcttgaag tttcaggatt tgatgtgaac 4140atgagaacag agcatgtcta
ctggtcatct gaaaacaagg gtgtcatcac aaaaatgtca 4200ttaacgcaca
gacacgagcc aaaacatttc atcactggtt tacgccgtcc atcagagctg
4260gcagtcgatt ggataacaca caacctctac tttgtccaag caaggaacat
cattaatgtc 4320tgtaacttcc accttgaacg ctgcgcacaa attttgaccg
ctgaaagtgg cttggagatc 4380aacagtcttg ccgttgatcc tgtcagaggt
gtccttttct ggagtgaaac aagccgcatc 4440gtctggaata tgcctaagag
ctccattaga cgcgcagaca tgaatggaaa aaatattgag 4500accattgttt
cagctaatgt gagctacgca ttggatcttg ctttagatcc aatcctcaat
4560catgtctatt gggtcgacaa aacattgaag gtcattgaaa gagctaacta
tgatggaact 4620cgccgacgtg tcatcttgac atcaaagttc catccaaaat
ctgttgcgct ctttgatggt 4680tctatctact ggtcggttga gtcgagtgga
tccccgatta caaagtgtgc tcttcaagga 4740ctttccacgg aatcgtactc
gtgcaatcaa atcccaatca aagttgtaga tccaataact 4800cacttcactc
tcatgcaacc agctctgcaa agaaacatat caaacgcttg taggaacatg
4860gagtgcagtc acatgtgcgt tctcagctcg acattaccat catgcatttg
tcgcaatgga 4920aagatagttc ctccaaaaac agcctgtacg gatagcaatt
atatgccaga aacccacttt 4980ttggaaacaa ctggaactgt tgatggtcaa
agtcctggat attcttggtc atcaatctgt 5040gcaacaatca tcttagtcgc
tttcatcgga acaactttct atgccttatt ctactactat 5100aactccaagt
acaacatgcg acgtctattt ccatcaattc attttaaaaa tccagccttc
5160aacctacagt caaaattcca ggctaatggg atgaccggtt tagccagtgg
caaccatatg 5220gctcacttgt catcgaagga ccatcatttc gaaaaccctc
tgcaagagag tcgtgaaggt 5280gaagtgagaa tcgttactcc aaatgaaatc
acgattagcc gagccgagac cagttggaca 5340tcagcccatc tagaagactc
aagctctata gaaacagaat acgcagacct tgttgtcgag 5400acaaacccca
aagctaattt gatatcgtga 5430305774DNABemisia tabaci 30atgattcaga
gggaatggag ctccatatcc aagggctcat ggtgcacagc attattagta 60gttatcgcag
tcttttgcac atttgtacaa tcatcttcat catatgaatg tgtcggacca
120tcccatttcg aatgtacaaa tcacaggtgt atatccatgg atttaaggtg
cgatggagat 180gacgattgta atgatggatc cgatgaacac ggttgtaatg
tggataaatc aaaaaatgaa 240acctgtgcta gtacccagtt tgattgcggt
cagggccaat gtataccacg gtcctgggtt 300tgtgatggga atgcagattg
tgaggacggt aaagatgaag gagctgtagg ctgcgctgaa 360agccattgcg
cagcgtctga gtgggagtgc cctcataacc atcgctgtat cccgaatgat
420tatatctgtg atggggatga tgattgtggt gacaattcag atgaagacga
ctgtacaggc 480aagaataatt ttacagagtg cacttcagcg tttggaaaat
ttctctgcaa aaataggaat 540cagtgcatcg atgattctct tctgtgcaac
gggcaccctg actgtaaaga tgggtcagat 600gagggtggtc actgtgcttc
gaaagctcaa gtagctactg actgtgcaaa gctaaactgt 660acccactcgt
gtgttgaaag cccagatgga ccggtttgcg tctgtggatc aggatatcac
720cttgaaggaa acgtctgtga agatattaat gagtgcttgg aatggggaac
atgtgaccaa 780atgtgtgaaa acacggtcgg aggttacatc tgtgaatgtg
aacctggata caaactggaa 840agtaacggac gcacatgcaa agcagaagag
ggtgaaggac ttctgatcta ttcaagcctc 900aaaaagatta agtctctata
tctgacatct cgcatatcaa tgacagttgc ctctgaggtt 960ccatatgcga
caggtgtgtc ttttgatggt aaacatgtct attggacgac cgtgcttgat
1020ggagttgaat ctattgttcg ggcaagtgag gatggatctc atgaaaccac
cattgttgat 1080tcaggtgttg gctcccctga agacctagct gtcgactggg
tgactggtaa catatacttc 1140actgatggcg agtaccaaca gatcggcatt
tgcacctaca acgaagagct ggttgaaacg 1200aaatgcgctg tcctccacaa
caaagacctg aacaagcccc gcgcaattgt tttaaaccca 1260gctgatgcgg
tcatgtactg gtctgattgg ggctttaaac cactcatcgc ccgttctgga
1320atggatggca cagacttcta tgagtttgta acgacagagc tccattggcc
caatggtctc 1380acaattgatc acggaaatcg gagagtatat tgggtcgatg
cgagactcgg aactgttgaa 1440actgttgatt ttcaaggccg tgatcggcgg
aaaatattaa ctgatctcaa tgatcatcct 1500ttcgcaatcg ctgtttttga
agataaaatc tattggagtg gatggacaaa ccaagaaata 1560gtagaatgta
ataagttcac cgggaaaaac cgagtacaag ttgtcaaaag tcggaaagac
1620aaaatttacg gtgtgcacat ttttcaccca actttgcaaa atcattcgct
accaaatccg 1680tgcgccggga agtgcagtga catctgtgct ctatctccat
cagcttcaag cggaggcaaa 1740ggctactctt gcttgtgccc tgacaataaa
atcttatccc cctctggaga atggtgccaa 1800gaacagccca aagaatctgt
cattgttagc attggaaact ttgttttcca gctgaaagtg 1860actcttggga
agcagtacat tcatccgctg cctgttaata acctccagtc tgtcagtgca
1920atcgtctaca actcctttga tggctctcta ttaatagctg acccagatgc
caaaatgatt 1980tactcgtatc aactgaacac ggacaccatg gagactctaa
ttgatctcaa agtgggctat 2040gtctcagcat tggcttacga tcccattggc
cggaatctct attggtgcga caaagaggcc 2100ggcactgtag aagtgttcag
tttcttctct cacagaagga agttgttgct ccgggaattt 2160gacgatgaaa
aacctttcgc catgactctc ataccagaag aaggcctgat gttcgtcatt
2220gccaaagccc atgaccacct tcacattgat cggatcaaca tggatggctc
actaagcaca 2280ctgactcaca tgaccagtct caagttgcaa ggaccggatg
tagctttgca ctatgacagt 2340gattctcgca gggtttattg ggctgatcac
tcagctggcc tcattgagag caccgatacg 2400aatggaaacg acaggcaagt
ataccgtgac gtatcatcac ctttagctct gaccgatgtg 2460gatagagacc
tttattggac atcagatggt cgcccacacc tgtactactc tgagaaaagc
2520aatgcaagca tgcccgtcag gaaaatcaac atggaacgat ttttgcgatc
accaaaggac 2580cactatcgga tgtttgttac tgcaatcatc cctgataaaa
ctactagaga tcatccttgc 2640caaactaaca atggcaagtg tagccatttt
tgcctcctca ccagtcgtaa tccaaagcat 2700gtatgcagtt gtcctgatgg
gatgaagctg gcggataatg gtcaggactg tgaagagatt 2760gctgcatgtg
gagcacatga ataccattgc acgacaggtg aatgcattcc aatgtcgaag
2820aaatgcgacc gaaacaagga ttgcccctat ggcgaagatg agaccttctg
cccagcacag 2880tgtgagactg atcagtttgc gtgctttgat ggacaaaaat
gtatcgatgc caaggataga 2940tgcaacatgc attttgattg tcacgaccac
tctgatgagg ctaactgcca aaatgtcacc 3000tgcgatcaat catacaactt
tttgtgccga atcggtgagt gcgtaagtca cgctgtgctg 3060tgcaataatg
aatggaattg caaggacggt agtgatgaag agaactgtac gacttccacg
3120tgcccttcca gcgagtttcg gtgtcattcc ggaacatgca taccgaagaa
ctgggtctgt 3180gatctggatg ccgattgccc cgaccaatca gatgaaaata
actgcagttt ctcaagaaaa 3240gaaaagtgca ccgaattctt gtgtcagagt
ggcatgtgtg tagcacagga acttgtctgt 3300aacggtcaaa ccgaatgtga
cgatggaagt gacgaattca attgcgatga gccggttcca 3360aaaacggcaa
acaaagagga tggttttata gataactgtg atgaagagaa ggagttcatg
3420tgcgagcctg gcaaatgcat taatcttata ttcaagtgta atggtgttaa
agattgcgag 3480aacggagctg atgagctaaa ctgcataggc tgtgaacaat
tcacttgcaa taatgggaaa 3540tgcatcactt atgatcttgt atgcaatgat
gatgatgact gtggtgactc atccgatgaa 3600agacctctca actcgtgccc
agacagcaaa gaaaacccag ctattgtgcc tgctcatatt 3660cccaatgtgt
gccatggatt tgtttgcaag aacggtgaat gccttgatga tttcagtcta
3720gtctgcaaca aaaagcaaga ttgtaaggac ggctcagatg aaggtggacg
atgcggttca 3780agctgtgatg tgacagcaaa ttgcagtcag atttgtcgtg
ataagccaaa tggacatgag 3840tgtgcgtgtg tccctggctt taaaattgct
gaggatggcc gcgattgcga ggatattgat 3900gagtgcaccg aattggagcc
gtgtagccag atgtgtttca acacctatgg aagttacacc 3960tgtgcctgtc
ttggtccaga ttatattaaa aagtcagatg ggtcctgcaa agccactggc
4020ccaaaattgc agtacgtctt tgctaccggt taccagattc gcactatatc
atacctaatg 4080accgatgtca aagtggcata ctatagtgca gatcttgaag
tttcaggatt tgatgtgaac 4140atgagaacag agcatgtcta ctggtcatct
gaaaacaagg gtgtcatcac aaaaatgtca 4200ttaacgcaca gacacgagcc
aaaacatttc atcactggtt tacgccgtcc atcagagctg 4260gcagtcgatt
ggataacaca caacctctac tttgtccaag caaggaacat cattaatgtc
4320tgtaacttcc accttgaacg ctgcgcacaa attttgaccg ctgaaagtgg
cttggagatc 4380aacagtcttg ccgttgatcc tgtcagaggt gtccttttct
ggagtgaaac aagccgcatc 4440gtctggaata tgcctaagag ctccattaga
cgcgcagaca tgaatggaaa aaatattgag 4500accattgttt cagctaatgt
gagctacgca ttggatcttg ctttagatcc aatcctcaat 4560catgtctatt
gggtcgacaa aacattgaag gtcattgaaa gagctaacta tgatggaact
4620cgccgacgtg tcatcttgac atcaaagttc catccaaaat ctgttgcgct
ctttgatggt 4680tctatctact ggtcggttga gtcgagtgga tccccgatta
caaagtgtgc tcttcaagga 4740ctttccacgg aatcgtactc gtgcaatcaa
atcccaatca aagttgtaga tccaataact 4800cacttcactc tcatgcaacc
agctctgcaa agaaacatat caaacgcttg taggaacatg 4860gagtgcagtc
acatgtgcgt tctcagctcg acattaccat catgcatttg tcgcaatgga
4920aagatagttc ctccaaaaac agcctgtacg gatagcaatt atatgccaga
aacccacttt 4980ttggaaacaa ctggaactgt tgatggtcaa agtcctggat
attcttggtc atcaatctgt 5040gcaacaatca tcttagtcgc tttcatcgga
acaactttct atgccttatt ctactactat 5100aactccaagt acaacatgcg
acgtctattt ccatcaattc attttaaaaa tccagccttc 5160aacctacagt
caaaattcca ggctaatggg atgaccggtt tagccagtgg caaccatatg
5220gctcacttgt catcgaagga ccatcatttc gaaaaccctc tgcaagagag
tcgtgaaggt 5280gaagtgagaa tcgttactcc aaatgaaatc acgattagcc
gagccgagac cagttggaca 5340tcagcccatc tagaagactc aagctctata
gaaacagaat acgcagacct tgttgtcgag 5400acaaacccca aagctaattt
gatatcgtga tactctttaa tcatttgaaa aatgcagtcg 5460tgtaaatatg
ttgtacagtt tgttttttgt tgaatctttt aatttatcta tgcatttgtt
5520gtttgtataa atttttatta ccatattttg gtgtcttcgg aactgaagtc
agtcaaattt 5580atcgagtcaa atttcatatc gatcagatca gtttatggtg
tttttatatt tgtttaaaga 5640agattgatcc atttttatca aaagcatctg
tcaattatct tccttagaaa aatgatacaa 5700tctaaatttt ttcaaattta
taatacaagg aaaaaaaaaa aacctatagt gaaatcacta 5760gtggaggatc cgcg
5774315370DNASpodoptera litura 31atgaggaaat atagtaacaa aatgaagtat
caaggcttgc tattgatagt tagtgtggcg 60tggtgttcag ctcagctcag cgatgacatg
cagatgtttg acccagaata catggcggaa 120gacaagttcc cctgcatggg
tggaggatgt atttcagtgt cacagtactg cgatggaaaa 180ttggactgtg
atgatggcag cgacgagaat ttttgcatcg aacacaagcc gttccaggag
240ttttgtaacg agatccacca gtacatgtgt cgggactcca cgaagtgtgt
tcctttatcc 300tggctgtgca acaatgagcc ggactgcgat gatggcagcg
atgagttcaa ctgcaccgca 360ctgccggctg taaacgtcaa ttcgacatgc
aaaggcttcc aatgtggaga cggcaaatgc 420atctccttcc tgtgggtgtg
tgacggtgtc tacgactgtg aagataaaag cgatgagtat 480gctgaggaac
tctgcaggca cgtatcccgt ccccacgcga tcgtcgacgg ttcatactgt
540caggaattgc acacaatgga cgaccgtaac tacaagtgtc tggacgcctc
cttctgcctg 600cccagcagta tgatgtgcga cggactccag gattgccgcg
atggcagcga tgagggacca 660ttttgcaaaa actggaatac aatgtgcgac
aacttcaaat gcatgggtaa cgacacgata 720tgttccccgg agaggtttgg
agcctcctgc ctctgcctgc cctcccactt catgcggcaa 780tacgattaca
tcaccaaaca gtgccaggat gtcaacgagt gcctcatgga gcacccaccc
840tgctcccata aatgcattaa cgccgatggc cattatattt gtgaatgcga
tcctggatac 900aaacgggatg tgtatggata tctttgttat gctactggtc
ccgaagcgat gctgttcttc 960aatactcgga acgatatcag atacctgaag
attaagacta aagaaatggt cacagtggct 1020accgacatca aagagggtca
tggcgtttca ttcgacggga cttacattta ttgggtggag 1080acagcacagg
gacatcagtc catcttcaaa gcacaactcg gagacgtcaa ggatacaaag
1140gaggtactgg taggtctcgg tctggaggac cctggcgaca tagcagtgga
ctacctgggc 1200gggaacatat acttcagtga tgcggagcga ggaatcatct
ccgcttgcag ggtcgacggc 1260tccatctgca ccacgatcaa gactcatgct
aacaacccac gatttgtcac tcttgatcct 1320aaaaatggta agatgtactg
ggcagactgg cacgagcgtc cagtgataat gtcagctcgg 1380atggacggca
gccgacacga tgagctggtg gacaacttgg agaactttgc caccggcctg
1440gccgtggacg cgccaaacgg gagactgtac ttcgttgaca aaactatcaa
agtcgtcatg 1500attgcggaga aacatgttta tgctttattt gaagagccct
tccaccatcc gtactccatc 1560tcagtgttcg agaacactgt gttctggagt
gactggacct ccaacagtat acagacgact 1620gacaaagtgc acgggaccgc
gcagaagagg aatgttctac tcaaacttga tacacctgta 1680ctaggcatgc
acatgtacca tccagtgctg atgaacacga catcgaaccc gtgcagcaac
1740aacaactgct cgcatctctg cttcgtcacc tccaacgcca cccacgtctg
cgcctgcccc 1800gatggcatgg agattgagaa taatcagtgc catcacgtag
gcaactaccg cgccaagtac 1860ctggtggtgg gaagtgggca gctgttcaca
aagatccagt acaacgcgct cggcaacccg 1920gagtgtcacg ccacgcactt
cgaaatcgga cgcgtgcagg ccatggcgta cgatcggtat 1980agagattcac
tattcatata cgacggccag cgacggacca tcaactacat aaacatgagt
2040gacttcactc tcggtatcac tcacgtgttg atctacaacg gactggagaa
cgtcgtcgac 2100atggattatg attacgtgac agataacctg tacgtgctgg
atgcaagtcg tcgtgtcgtt 2160gaagctgtgt ctctgaggac tcagaagcgc
gccatagtac atcgattcga cattcaagaa 2220ctgcctgtta gcttctgtat
tctttcggac tacgggagaa tgttggtggc cgtggtggag 2280agtgagatgc
acaacaccat tcatatcgac agcatcgggc tcgacgggac ccaacggaga
2340cacgtcatca tgaacaattt gaaaggtcct catattcgat tgcggtacgt
gccagatttt 2400attgggcgcg actggaccca acggagacac gtcatcatga
acaatttgaa aggtcctcat 2460attcgattgc ggtacgtgcc agaaactgag
caggtgttca tatccgacga gagcaacggc 2520atcatcgatt tcatacatcc
tgaaggcacc gggcgggaga actatcggga gctgacctcg 2580acggtgacca
gcctcgccat agcagataac tacgtcttct ggacagaccg aaagaccccc
2640agactcttct ggtctgacat acacgaagcc tcgccgaaga taaggaggat
tgatctggca 2700cttttcccaa acactacgca acttctgatc caagcaacga
actcgctccc tgatccaaaa 2760gaccctcttc tgaaccatcc ctgtctaaag
aatccttgtt ctgacgtctg tgtccaactt 2820ccccatgata ctccagaaga
tcacccgaaa ctagcaaact tcgagatgaa gtacaagtgt 2880ttgtgtcctc
ctgggttgtt ggtgaatggt aaccagtgcg ccaaacctgc gacatgtggt
2940tcggatgaga tactgtgcca taggagtaat atttgtgtga agcaagatgc
caggtgtgat 3000ggaaaggccg actgtccgaa gaatgaagac gaggaaggtt
gtatagtgga tccggcaaac 3060atatgttcat cagacgaaat attctgtcgc
ggcttgtgcg taaacaggga caaagccacg 3120acgtgttcta ccggagaaaa
aactaataaa gcactacctt cgaacaactg cagcagcaca 3180gaattccagt
gcacggacac ctcaatctgc atctctcggt cgcaggtctg tgaccaacac
3240atcgactgtc ctaatggatc ggatgagcag ctcttagagt gcgacatcta
ctcttgtcat 3300gaaactgagt tcatgtgtgc gtccggctca tgtatagtga
aaacgtggac atgtgatgga 3360gaccgtgact gtaatgatgg atctgatgaa
atcaactgcg tgaacatgac ttgcggcctg 3420ggcttctacc agtgcagaga
cagggagtgc gtggagttat cgaagaggtg tgacggacat 3480cgggattgct
cggattactc cgacgaagag gactgtgatg agcctcaaat tgtcgagacg
3540atagaagaag ccccgaaatg tgctgcgtgg gaatatactt gtgaaaaaaa
tactagcatt 3600tgtttgcctg aaaccgctcg atgcaacatg aagacggact
gcccgggtgg cacggatgag 3660cacggttgcg accaccgctg tgccccgaag
ggaatgtttg cgtgcggcca acaggtcact 3720tgtatcacaa tgaacaaggt
ctgcaacgga cgactggatt gtgatgacgg atccgatgag 3780acgcctgatg
cttgttctca ggtgaacagg acctctcacc tgttcccagt agctcggaca
3840ttcactgaat gtactgaggg ttataagtgt aacaacggac agtgcattga
gtggtcacag 3900ttgtgtgaca agaagaggga ctgcatcgat gggacagatg
aaaatggact ttgcgatact 3960gcatgcgcaa acagcacatg tacgttcatg
tgtcagccga ccccattcgg acgtcgctgt 4020ctgtgcccct ttggcttcca
aatttccaag gatcagttct cgtgcgagga catcgacgag 4080tgcactgaag
atgtctgctc tcaagggtgt atcaacgtgc cagggtcctt cctgtgctgg
4140tgccatcatg gttatgccat caggaggaca gaccgtcgct attgcaaggc
gatccgcggc 4200aacatgtcga tcctgtacgt gtcgggtaac accgtgcggt
ctatctcagc agacgggtac 4260ggtgctatcg agtataccga tactaatgcc
tcggccatca ctgatatgga ttataatgtt 4320cggcagaaga agctatacgt
gacctcggaa gcgggtggca agttgttaga agtgaatgag 4380acgcagaatg
tgatcgctgt tacgaacgtt gggaaaccat cgagggtggc agtggactgg
4440gtgaccggca acgtgtactt cgtggacacg acgccatttg accagcgcat
acgagtctgt 4500catgtcaaga ggaagcgctg cgcttccctg ctgaaacttc
cttctgatgc cacggtgaca 4560gcgttgatag tagagcccag ttccagccgc
atgttctact gcgtcactcg gaagctggag 4620tcggtgatct ggacagcgaa
cctggctggc agacacgtga cggatctggc tactgtacgg 4680aactgcaccg
gtctggccgc agactccttc aagaagaagc tgtacgtcgc agagacgggc
4740ccagcgcata ttattaggat ggattatgag ggggagaatt tcaacaaaat
cctctcagat 4800cacccacgtc tgcaagcacc gcatggacta gaaatattcg
aagactacat ctactacttg 4860gaagccaact cgttccgcct cagccgctgc
cagctgtacg gggccaaaca ttgtgaaact 4920tatgtgtacc gtgtgtttga
cgccaacacg ttcgtcattc gccacgagag catccaacgc 4980gatgacatag
tcaacgaatg tgaagatgtc gtttgtgaca acatttgtgc cgtggacgaa
5040gatgggccca aatgtttgtg tgacgatgga gccttggcca agagtgggaa
gtgtcctgtg 5100gttgataaga aattggtacc tttattcaac ggctggtcgt
acgagcagct ggcatcggct 5160cgcagcatcc ccttcaccgt caccttggga
gtactgaccc tgatcgccat ctacctctgc 5220gtcttcgtat actaccattg
tatatacata cccaggaaga gaatgttagc atctgcttac 5280actgaagttc
ggttccagaa cacacaaaat acgtcctatc ctgaatcaga cccggtagtg
5340gagatgcatc cctcaaggta tctaccttag 5370325370DNASpodoptera litura
32atgaggaaat atagtaacaa aatgaagtat caaggcttgc tattgatagt tagtgtggcg
60tggtgttcag ctcagctcag cgatgacatg cagatgtttg acccagaata catggcggaa
120gacaagttcc cctgcatggg tggaggatgt atttcagtgt cacagtactg
cgatggaaaa 180ttggactgtg atgatggcag cgacgagaat ttttgcatcg
aacacaagcc gttccaggag 240ttttgtaacg agatccacca gtacatgtgt
cgggactcca cgaagtgtgt tcctttatcc 300tggctgtgca acaatgagcc
ggactgcgat gatggcagcg atgagttcaa ctgcaccgca 360ctgccggctg
taaacgtcaa ttcgacatgc aaaggcttcc aatgtggaga cggcaaatgc
420atctccttcc tgtgggtgtg tgacggtgtc tacgactgtg aagataaaag
cgatgagtat 480gctgaggaac tctgcaggca cgtatcccgt ccccacgcga
tcgtcgacgg ttcatactgt 540caggaattgc acacaatgga cgaccgtaac
tacaagtgtc tggacgcctc cttctgcctg 600cccagcagta tgatgtgcga
cggactccag gattgccgcg atggcagcga tgagggacca 660ttttgcaaaa
actggaatac aatgtgcgac aacttcaaat gcatgggtaa cgacacgata
720tgttccccgg agaggtttgg agcctcctgc ctctgcctgc cctcccactt
catgcggcaa 780tacgattaca tcaccaaaca gtgccaggat gtcaacgagt
gcctcatgga gcacccaccc 840tgctcccata aatgcattaa cgccgatggc
cattatattt gtgaatgcga tcctggatac 900aaacgggatg tgtatggata
tctttgttat gctactggtc ccgaagcgat gctgttcttc 960aatactcgga
acgatatcag atacctgaag attaagacta aagaaatggt cacagtggct
1020accgacatca aagagggtca tggcgtttca ttcgacggga cttacattta
ttgggtggag 1080acagcacagg gacatcagtc catcttcaaa gcacaactcg
gagacgtcaa ggatacaaag 1140gaggtactgg taggtctcgg tctggaggac
cctggcgaca tagcagtgga ctacctgggc 1200gggaacatat acttcagtga
tgcggagcga ggaatcatct ccgcttgcag ggtcgacggc 1260tccatctgca
ccacgatcaa gactcatgct aacaacccac gatttgtcac tcttgatcct
1320aaaaatggta agatgtactg ggcagactgg cacgagcgtc cagtgataat
gtcagctcgg 1380atggacggca gccgacacga tgagctggtg gacaacttgg
agaactttgc caccggcctg 1440gccgtggacg cgccaaacgg
gagactgtac ttcgttgaca aaactatcaa agtcgtcatg 1500attgcggaga
aacatgttta tgctttattt gaagagccct tccaccatcc gtactccatc
1560tcagtgttcg agaacactgt gttctggagt gactggacct ccaacagtat
acagacgact 1620gacaaagtgc acgggaccgc gcagaagagg aatgttctac
tcaaacttga tacacctgta 1680ctaggcatgc acatgtacca tccagtgctg
atgaacacga catcgaaccc gtgcagcaac 1740aacaactgct cgcatctctg
cttcgtcacc tccaacgcca cccacgtctg cgcctgcccc 1800gatggcatgg
agattgagaa taatcagtgc catcacgtag gcaactaccg cgccaagtac
1860ctggtggtgg gaagtgggca gctgttcaca aagatccagt acaacgcgct
cggcaacccg 1920gagtgtcacg ccacgcactt cgaaatcgga cgcgtgcagg
ccatggcgta cgatcggtat 1980agagattcac tattcatata cgacggccag
cgacggacca tcaactacat aaacatgagt 2040gacttcactc tcggtatcac
tcacgtgttg atctacaacg gactggagaa cgtcgtcgac 2100atggattatg
attacgtgac agataacctg tacgtgctgg atgcaagtcg tcgtgtcgtt
2160gaagctgtgt ctctgaggac tcagaagcgc gccatagtac atcgattcga
cattcaagaa 2220ctgcctgtta gcttctgtat tctttcggac tacgggagaa
tgttggtggc cgtggtggag 2280agtgagatgc acaacaccat tcatatcgac
agcatcgggc tcgacgggac ccaacggaga 2340cacgtcatca tgaacaattt
gaaaggtcct catattcgat tgcggtacgt gccagatttt 2400attgggcgcg
actggaccca acggagacac gtcatcatga acaatttgaa aggtcctcat
2460attcgattgc ggtacgtgcc agaaactgag caggtgttca tatccgacga
gagcaacggc 2520atcatcgatt tcatacatcc tgaaggcacc gggcgggaga
actatcggga gctgacctcg 2580acggtgacca gcctcgccat agcagataac
tacgtcttct ggacagaccg aaagaccccc 2640agactcttct ggtctgacat
acacgaagcc tcgccgaaga taaggaggat tgatctggca 2700cttttcccaa
acactacgca acttctgatc caagcaacga actcgctccc tgatccaaaa
2760gaccctcttc tgaaccatcc ctgtctaaag aatccttgtt ctgacgtctg
tgtccaactt 2820ccccatgata ctccagaaga tcacccgaaa ctagcaaact
tcgagatgaa gtacaagtgt 2880ttgtgtcctc ctgggttgtt ggtgaatggt
aaccagtgcg ccaaacctgc gacatgtggt 2940tcggatgaga tactgtgcca
taggagtaat atttgtgtga agcaagatgc caggtgtgat 3000ggaaaggccg
actgtccgaa gaatgaagac gaggaaggtt gtatagtgga tccggcaaac
3060atatgttcat cagacgaaat attctgtcgc ggcttgtgcg taaacaggga
caaagccacg 3120acgtgttcta ccggagaaaa aactaataaa gcactacctt
cgaacaactg cagcagcaca 3180gaattccagt gcacggacac ctcaatctgc
atctctcggt cgcaggtctg tgaccaacac 3240atcgactgtc ctaatggatc
ggatgagcag ctcttagagt gcgacatcta ctcttgtcat 3300gaaactgagt
tcatgtgtgc gtccggctca tgtatagtga aaacgtggac atgtgatgga
3360gaccgtgact gtaatgatgg atctgatgaa atcaactgcg tgaacatgac
ttgcggcctg 3420ggcttctacc agtgcagaga cagggagtgc gtggagttat
cgaagaggtg tgacggacat 3480cgggattgct cggattactc cgacgaagag
gactgtgatg agcctcaaat tgtcgagacg 3540atagaagaag ccccgaaatg
tgctgcgtgg gaatatactt gtgaaaaaaa tactagcatt 3600tgtttgcctg
aaaccgctcg atgcaacatg aagacggact gcccgggtgg cacggatgag
3660cacggttgcg accaccgctg tgccccgaag ggaatgtttg cgtgcggcca
acaggtcact 3720tgtatcacaa tgaacaaggt ctgcaacgga cgactggatt
gtgatgacgg atccgatgag 3780acgcctgatg cttgttctca ggtgaacagg
acctctcacc tgttcccagt agctcggaca 3840ttcactgaat gtactgaggg
ttataagtgt aacaacggac agtgcattga gtggtcacag 3900ttgtgtgaca
agaagaggga ctgcatcgat gggacagatg aaaatggact ttgcgatact
3960gcatgcgcaa acagcacatg tacgttcatg tgtcagccga ccccattcgg
acgtcgctgt 4020ctgtgcccct ttggcttcca aatttccaag gatcagttct
cgtgcgagga catcgacgag 4080tgcactgaag atgtctgctc tcaagggtgt
atcaacgtgc cagggtcctt cctgtgctgg 4140tgccatcatg gttatgccat
caggaggaca gaccgtcgct attgcaaggc gatccgcggc 4200aacatgtcga
tcctgtacgt gtcgggtaac accgtgcggt ctatctcagc agacgggtac
4260ggtgctatcg agtataccga tactaatgcc tcggccatca ctgatatgga
ttataatgtt 4320cggcagaaga agctatacgt gacctcggaa gcgggtggca
agttgttaga agtgaatgag 4380acgcagaatg tgatcgctgt tacgaacgtt
gggaaaccat cgagggtggc agtggactgg 4440gtgaccggca acgtgtactt
cgtggacacg acgccatttg accagcgcat acgagtctgt 4500catgtcaaga
ggaagcgctg cgcttccctg ctgaaacttc cttctgatgc cacggtgaca
4560gcgttgatag tagagcccag ttccagccgc atgttctact gcgtcactcg
gaagctggag 4620tcggtgatct ggacagcgaa cctggctggc agacacgtga
cggatctggc tactgtacgg 4680aactgcaccg gtctggccgc agactccttc
aagaagaagc tgtacgtcgc agagacgggc 4740ccagcgcata ttattaggat
ggattatgag ggggagaatt tcaacaaaat cctctcagat 4800cacccacgtc
tgcaagcacc gcatggacta gaaatattcg aagactacat ctactacttg
4860gaagccaact cgttccgcct cagccgctgc cagctgtacg gggccaaaca
ttgtgaaact 4920tatgtgtacc gtgtgtttga cgccaacacg ttcgtcattc
gccacgagag catccaacgc 4980gatgacatag tcaacgaatg tgaagatgtc
gtttgtgaca acatttgtgc cgtggacgaa 5040gatgggccca aatgtttgtg
tgacgatgga gccttggcca agagtgggaa gtgtcctgtg 5100gttgataaga
aattggtacc tttattcaac ggctggtcgt acgagcagct ggcatcggct
5160cgcagcatcc ccttcaccgt caccttggga gtactgaccc tgatcgccat
ctacctctgc 5220gtcttcgtat actaccattg tatatacata cccaggaaga
gaatgttagc atctgcttac 5280actgaagttc ggttccagaa cacacaaaat
acgtcctatc ctgaatcaga cccggtagtg 5340gagatgcatc cctcaaggta
tctaccttag 5370335341DNAPhyllotreta cruciferae 33tcagcagttt
tcgcacccga aggaaattgc acctccgacc agttccaatg ctccgaccag 60cgatgcataa
actttaaagc cagatgcgac tcaaaactgg attgcccgga cggatcggac
120gaattagaat gcgatctgca tcgctgcgat gagccggagt tcttccgctg
taaaaataac 180agatgcatca gtcacgcttt cgtctgcgat aaacagaacg
attgcgagga cttctccgac 240gaggcgaact gcgacagctt ccagtctttg
gtgtcttcgg cttgcgaaga gggccactgg 300aagtgcgccg acaaattgtg
catacccaac gattgggtgt gcgacggcac gccggaatgc 360atcgacggat
ccgacgagac catcggctgc agcacccaga tcgcctgcga cgggttccgc
420tgcaagaaca agcactgcgt gcccaaagag tgggtctgcg acaaccacaa
cgattgcatc 480gataacagcg acgaggagga ctgcgagaac cacatcccca
tcgaccagtg cacgctggac 540aagcgcaagt tcctctgcca caacaacgtg
acttgcattg acaccgagtc ggtttgcgat 600ggagtcaata attgtccaga
caattcggac gaatccctgc aatgtaacgc gtcagtgaag 660gcttgcaaag
gcgatcacca gtgctcgcac gtttgcatcc cgctgcccga aggccccaag
720tgcgtctgtc ccgcggggta ccacacgctc gacgagaaaa actgcgagga
tatcaacgaa 780tgcgaaacat acggtatatg cgatcaaatg tgccggaaca
cgccgggttc gtacgaatgc 840tactgcgaca aggcgtaccg tttggcggag
gacaagaaaa cctgcaaggc ggagggcgag 900gtgggcatca tggtgttcag
ctcgaaggat caaatcagag cggtggcgct ggattcgacc 960agctacatca
tcgtcgccaa gaacttgaag aaagtggtgg gcgtcgattt cgacggtcgc
1020cgggtctact ggacggagat atacggggga tacgagaaca tcgccaggag
cgccgaggac 1080gggtctcaga aggaggtgtt agtaacggct ggcgtagatc
taccagagga tttgtccatc 1140gattggttga cgggtaacat ttatttcacc
gatggcgaga agcagcacat cggagtttgc 1200tcgccggacg ggacccattg
cgctgtttta atcaataagg acatcaggaa acccagaggg 1260atcgttctta
acgtggtgga cggcgacatg tactggacgg attgggaaga tccggcgcga
1320atcggttact cgctaatgga cggatcaaat gataagcctt tcgtggaaaa
agacatacat 1380tggcccaacg gtttagcatt ggatcatcct aatagtagat
tgtactggac ggatgctagg 1440aagatgacat tggagagcat acgtttagat
ggaactgatc gaaggataat attagaagac 1500atcgtgaagc atccgtacgc
catagcagta ttcgaggata aattgtactg gtcggattgg 1560gcgacgcgaa
ccatccaaca gtgcgacaaa ttcgacggga agaaccacaa aacgttgatc
1620gaagagaaag agctcatcta cgggatcagc atattccatt cggcgcagca
gaagcgcagc 1680aaaaacccct gcgagatggc gctgtgcagc gacttgtgct
tactgaaagg tacatcgtac 1740agctgcgctt gtccacagaa caaggtctta
caatccgacg ggcttttttg taaagaatta 1800tcgcctacag aaactcttat
cgccgctcat cgggatatgc tagtacatat agagcatccg 1860attcttggta
ggcatgttgt tacagctcta cccggcgcca ctgcagacat agaaagcatg
1920acattcgaca gctataagaa tgtactgtac gtcagcgact tcaagaccag
gaggatcagt 1980actttgcaca tgaagaccgg tagtagcaaa gttatggaca
tttccgattt gggtagaatc 2040agcgctatgg atttcgattc taaaagcaac
aatctttaca tctgcgattc ggtacgcaaa 2100gtggtggaag taatcagtct
caacacgatg gcaaggcgga ttttaatcca cgacacgttc 2160ggagaaatcc
cccaaagtat cgcgttagtc cccgacgaag gggtgatgtt tgtaagcttc
2220ggaggcgagg gacccagcca catcgatcgc ttctccatgg acggtacgga
tcgaactcac 2280tctatagaca ccaaactaac ggggccagtg tcactggcgt
acgatcccga tttacacagg 2340gtgttcttcg ccgacgccac caacggctta
atcgaatcca ccaacgtgga cggcgacgat 2400cgcatccact tcagaaccgt
cgattctcac cccatcagct tgctggtgct caaagacgat 2460gtgttctggg
tgaacgtgca ctcgaagcag ctctactgga cctcgaagaa agtcccatcg
2520aattacgaca agaaaattac gctggcgttc cccgacgatc cggacaaggt
gcacttggtg 2580tcagtaacgt cgcgccatgt agagagcaac ttatgtcgca
tcaacaacaa cggttgcagc 2640catctgtgct tgcagtcgca gaagtccatc
gtgtgccagt gcccgatcgg ctgggagctg 2700aaggaggaca accgcacctg
cgccaagaga gtgggttgca tcggcggcga gtttttgtgc 2760caccactcca
acacttgcat tttgaaaagc ctcagatgca acggccacaa ggactgcttg
2820tttggagaag acgagagcga ttgcgcagct gcagcgaaat gcggaggaag
cgagttccaa 2880tgcgccaacg gcgattgcat agctgcggat ctggcctgca
accatcggta tgattgcaag 2940gataaatctg acgagcacgg ttgcgatgat
gttaagaacg gcactcgatg cccgccagat 3000cacttcacct gctcgaacgg
ggattgcatc agccagcatt tccactgcga tggcgtctcc 3060gattgcacag
acaactcaga cgagattcac tgccaggtga acgattgtaa tgcaacgcaa
3120tttaggtgcg attcgggctc ttgcattccc aaggaatggg agtgcgatca
cgattacgat 3180tgcatggata gttctgatga gcattgcaat gcgctgtgcc
cggagagcca cttcaaatgc 3240gacaacggcc tgtgcgtgga caagaagtta
atgtgcgacg gtttcgacaa ctgcggcgac 3300cactccgacg agaagatgca
catgtgccac cagagggccg cgcacaactg caccgcctac 3360gagacggcct
gctcgtccaa cgcctccatt tgcattcctt tggaagcgaa atgcgacggg
3420aaatccgact gtcccaagca cgaagacgag atcggctgcg ccatgtgcgc
cgacgaggat 3480ttcgagtgcc gcaacaagca tttgaaggac tgcattccga
ggatgtggct gtgcgacggc 3540atgaacgatt gcggggacaa cagcgacgag
gacttggtga tgtgcagcaa aaagaacaga 3600accatgttcg ctccggccca
agtaccctgc accgatggct tcaggtgcga taacggcaac 3660tgcatcaacg
catccttgct ctgcaacggg aaacacgact gctatgatgg ctctgacgaa
3720ggaggcttgt gcaccgggtc ctgcgaaggc tctaagaatc cttgtaatca
catctgcatc 3780aaaacaccga aaggtcctaa atgccagtgc aggtcgggct
acaagttgat gggcgatggt 3840aaaacgtgcg ttgatgagaa cgaatgcgag
gtctatcctc ccatatgcag ccaaatttgc 3900cgcaacagag aaggcggtta
cacttgcgat tgcttccaca acttttattt aagaaaggac 3960atgaaatcct
gcaaagcggc gggttccgac atgttgatct acttcaacgt gaacggcaat
4020caaatctacg aaatcgcgcc gaagaacaat tcgatgagcg tcgtccagga
agagtcgatg 4080ctgaagatca cgagtctgga tactctggtg gactctaagg
agctcttctt cagcgtgcag 4140gacaccggcg ccatctacaa gctggatccg
gctacaggaa ccatgcacta catcaagaac 4200ttgggcgagc cgaagctgat
agccgtggat tggtcgacgg ggaatgttta ttatcacaac 4260gcccaatcgg
acgcgaaatc gatcggggtg tgcaattacg aggagaagtg cgccaaattg
4320atcgacatcg acgcgcatcg acaggtgtcc gcattggctg tggattcttt
caacaaagtt 4380ttgttctatg tggtcagatc gtggacgata ttcgcatcgc
ccagctacgt tatatacaaa 4440accgatttgg acggtagcaa cgtggtggaa
ctggtcaaaa caaatatagg caacgtggaa 4500cacatcacct acgatttgaa
caaacgacag ctgtacttcc acgacgagaa cacccagcaa 4560atcaacgtga
tcggctacga agggggccgc accaaatccc tcttctacaa cgtgacttta
4620atcgaagggc tcagactgtt cgaagataat ttgtactact tgcgccagga
cgggttcctc 4680atcagatgcc agttgttcga agaggccgcc tgtcactacg
gtttcaactt gcacagtttg 4740gctagggacg agttcgtaat ctcgcacaga
tcccttcagc ccttcgttgg gcacgtctgc 4800gatgggcatg cttgccccta
catgtgcgtg gctggacaag agggctattt gtgcgtttgc 4860catgacggct
cgaccagtgg cgaatgcggc acaccgcaag acgagaacgg cgagaacaaa
4920ttcaaagtaa cttccatcga gggaaaatcc agatcttcgg cgtttttgtc
cggtttcgcg 4980gtgtttgggg tactagtagc atgcgtcatg attatacttg
gggcttatta catgataaag 5040aggagaagcg gtgataacag taccgatttg
agcatcgtgc gatttcaaaa tccgctttac 5100ggaagacccg tggaggacga
gaaacctatt ttggagcccg ggaagcacga gtacgtgaat 5160tgtttgtacc
aacacaaagc ggatggtggc gaaaatagca atactgaacc caaaacgttc
5220aacgtctgtg atgataattt gtgttaattt attgtttttt tatgttttac
gttaattagg 5280tgcattattt gattgttaat tctatttata acacattatt
atatgtatat acaaattgtt 5340a 5341345341DNAPhyllotreta cruciferae
34tcagcagttt tcgcacccga aggaaattgc acctccgacc agttccaatg ctccgaccag
60cgatgcataa actttaaagc cagatgcgac tcaaaactgg attgcccgga cggatcggac
120gaattagaat gcgatctgca tcgctgcgat gagccggagt tcttccgctg
taaaaataac 180agatgcatca gtcacgcttt cgtctgcgat aaacagaacg
attgcgagga cttctccgac 240gaggcgaact gcgacagctt ccagtctttg
gtgtcttcgg cttgcgaaga gggccactgg 300aagtgcgccg acaaattgtg
catacccaac gattgggtgt gcgacggcac gccggaatgc 360atcgacggat
ccgacgagac catcggctgc agcacccaga tcgcctgcga cgggttccgc
420tgcaagaaca agcactgcgt gcccaaagag tgggtctgcg acaaccacaa
cgattgcatc 480gataacagcg acgaggagga ctgcgagaac cacatcccca
tcgaccagtg cacgctggac 540aagcgcaagt tcctctgcca caacaacgtg
acttgcattg acaccgagtc ggtttgcgat 600ggagtcaata attgtccaga
caattcggac gaatccctgc aatgtaacgc gtcagtgaag 660gcttgcaaag
gcgatcacca gtgctcgcac gtttgcatcc cgctgcccga aggccccaag
720tgcgtctgtc ccgcggggta ccacacgctc gacgagaaaa actgcgagga
tatcaacgaa 780tgcgaaacat acggtatatg cgatcaaatg tgccggaaca
cgccgggttc gtacgaatgc 840tactgcgaca aggcgtaccg tttggcggag
gacaagaaaa cctgcaaggc ggagggcgag 900gtgggcatca tggtgttcag
ctcgaaggat caaatcagag cggtggcgct ggattcgacc 960agctacatca
tcgtcgccaa gaacttgaag aaagtggtgg gcgtcgattt cgacggtcgc
1020cgggtctact ggacggagat atacggggga tacgagaaca tcgccaggag
cgccgaggac 1080gggtctcaga aggaggtgtt agtaacggct ggcgtagatc
taccagagga tttgtccatc 1140gattggttga cgggtaacat ttatttcacc
gatggcgaga agcagcacat cggagtttgc 1200tcgccggacg ggacccattg
cgctgtttta atcaataagg acatcaggaa acccagaggg 1260atcgttctta
acgtggtgga cggcgacatg tactggacgg attgggaaga tccggcgcga
1320atcggttact cgctaatgga cggatcaaat gataagcctt tcgtggaaaa
agacatacat 1380tggcccaacg gtttagcatt ggatcatcct aatagtagat
tgtactggac ggatgctagg 1440aagatgacat tggagagcat acgtttagat
ggaactgatc gaaggataat attagaagac 1500atcgtgaagc atccgtacgc
catagcagta ttcgaggata aattgtactg gtcggattgg 1560gcgacgcgaa
ccatccaaca gtgcgacaaa ttcgacggga agaaccacaa aacgttgatc
1620gaagagaaag agctcatcta cgggatcagc atattccatt cggcgcagca
gaagcgcagc 1680aaaaacccct gcgagatggc gctgtgcagc gacttgtgct
tactgaaagg tacatcgtac 1740agctgcgctt gtccacagaa caaggtctta
caatccgacg ggcttttttg taaagaatta 1800tcgcctacag aaactcttat
cgccgctcat cgggatatgc tagtacatat agagcatccg 1860attcttggta
ggcatgttgt tacagctcta cccggcgcca ctgcagacat agaaagcatg
1920acattcgaca gctataagaa tgtactgtac gtcagcgact tcaagaccag
gaggatcagt 1980actttgcaca tgaagaccgg tagtagcaaa gttatggaca
tttccgattt gggtagaatc 2040agcgctatgg atttcgattc taaaagcaac
aatctttaca tctgcgattc ggtacgcaaa 2100gtggtggaag taatcagtct
caacacgatg gcaaggcgga ttttaatcca cgacacgttc 2160ggagaaatcc
cccaaagtat cgcgttagtc cccgacgaag gggtgatgtt tgtaagcttc
2220ggaggcgagg gacccagcca catcgatcgc ttctccatgg acggtacgga
tcgaactcac 2280tctatagaca ccaaactaac ggggccagtg tcactggcgt
acgatcccga tttacacagg 2340gtgttcttcg ccgacgccac caacggctta
atcgaatcca ccaacgtgga cggcgacgat 2400cgcatccact tcagaaccgt
cgattctcac cccatcagct tgctggtgct caaagacgat 2460gtgttctggg
tgaacgtgca ctcgaagcag ctctactgga cctcgaagaa agtcccatcg
2520aattacgaca agaaaattac gctggcgttc cccgacgatc cggacaaggt
gcacttggtg 2580tcagtaacgt cgcgccatgt agagagcaac ttatgtcgca
tcaacaacaa cggttgcagc 2640catctgtgct tgcagtcgca gaagtccatc
gtgtgccagt gcccgatcgg ctgggagctg 2700aaggaggaca accgcacctg
cgccaagaga gtgggttgca tcggcggcga gtttttgtgc 2760caccactcca
acacttgcat tttgaaaagc ctcagatgca acggccacaa ggactgcttg
2820tttggagaag acgagagcga ttgcgcagct gcagcgaaat gcggaggaag
cgagttccaa 2880tgcgccaacg gcgattgcat agctgcggat ctggcctgca
accatcggta tgattgcaag 2940gataaatctg acgagcacgg ttgcgatgat
gttaagaacg gcactcgatg cccgccagat 3000cacttcacct gctcgaacgg
ggattgcatc agccagcatt tccactgcga tggcgtctcc 3060gattgcacag
acaactcaga cgagattcac tgccaggtga acgattgtaa tgcaacgcaa
3120tttaggtgcg attcgggctc ttgcattccc aaggaatggg agtgcgatca
cgattacgat 3180tgcatggata gttctgatga gcattgcaat gcgctgtgcc
cggagagcca cttcaaatgc 3240gacaacggcc tgtgcgtgga caagaagtta
atgtgcgacg gtttcgacaa ctgcggcgac 3300cactccgacg agaagatgca
catgtgccac cagagggccg cgcacaactg caccgcctac 3360gagacggcct
gctcgtccaa cgcctccatt tgcattcctt tggaagcgaa atgcgacggg
3420aaatccgact gtcccaagca cgaagacgag atcggctgcg ccatgtgcgc
cgacgaggat 3480ttcgagtgcc gcaacaagca tttgaaggac tgcattccga
ggatgtggct gtgcgacggc 3540atgaacgatt gcggggacaa cagcgacgag
gacttggtga tgtgcagcaa aaagaacaga 3600accatgttcg ctccggccca
agtaccctgc accgatggct tcaggtgcga taacggcaac 3660tgcatcaacg
catccttgct ctgcaacggg aaacacgact gctatgatgg ctctgacgaa
3720ggaggcttgt gcaccgggtc ctgcgaaggc tctaagaatc cttgtaatca
catctgcatc 3780aaaacaccga aaggtcctaa atgccagtgc aggtcgggct
acaagttgat gggcgatggt 3840aaaacgtgcg ttgatgagaa cgaatgcgag
gtctatcctc ccatatgcag ccaaatttgc 3900cgcaacagag aaggcggtta
cacttgcgat tgcttccaca acttttattt aagaaaggac 3960atgaaatcct
gcaaagcggc gggttccgac atgttgatct acttcaacgt gaacggcaat
4020caaatctacg aaatcgcgcc gaagaacaat tcgatgagcg tcgtccagga
agagtcgatg 4080ctgaagatca cgagtctgga tactctggtg gactctaagg
agctcttctt cagcgtgcag 4140gacaccggcg ccatctacaa gctggatccg
gctacaggaa ccatgcacta catcaagaac 4200ttgggcgagc cgaagctgat
agccgtggat tggtcgacgg ggaatgttta ttatcacaac 4260gcccaatcgg
acgcgaaatc gatcggggtg tgcaattacg aggagaagtg cgccaaattg
4320atcgacatcg acgcgcatcg acaggtgtcc gcattggctg tggattcttt
caacaaagtt 4380ttgttctatg tggtcagatc gtggacgata ttcgcatcgc
ccagctacgt tatatacaaa 4440accgatttgg acggtagcaa cgtggtggaa
ctggtcaaaa caaatatagg caacgtggaa 4500cacatcacct acgatttgaa
caaacgacag ctgtacttcc acgacgagaa cacccagcaa 4560atcaacgtga
tcggctacga agggggccgc accaaatccc tcttctacaa cgtgacttta
4620atcgaagggc tcagactgtt cgaagataat ttgtactact tgcgccagga
cgggttcctc 4680atcagatgcc agttgttcga agaggccgcc tgtcactacg
gtttcaactt gcacagtttg 4740gctagggacg agttcgtaat ctcgcacaga
tcccttcagc ccttcgttgg gcacgtctgc 4800gatgggcatg cttgccccta
catgtgcgtg gctggacaag agggctattt gtgcgtttgc 4860catgacggct
cgaccagtgg cgaatgcggc acaccgcaag acgagaacgg cgagaacaaa
4920ttcaaagtaa cttccatcga gggaaaatcc agatcttcgg cgtttttgtc
cggtttcgcg 4980gtgtttgggg tactagtagc atgcgtcatg attatacttg
gggcttatta catgataaag 5040aggagaagcg gtgataacag taccgatttg
agcatcgtgc gatttcaaaa tccgctttac 5100ggaagacccg tggaggacga
gaaacctatt ttggagcccg ggaagcacga gtacgtgaat 5160tgtttgtacc
aacacaaagc ggatggtggc gaaaatagca atactgaacc caaaacgttc
5220aacgtctgtg atgataattt gtgttaattt attgtttttt tatgttttac
gttaattagg 5280tgcattattt gattgttaat tctatttata acacattatt
atatgtatat acaaattgtt 5340a 5341355186DNANezara viridula
35ctggaacgag tttaatttat tgtttgtcac aacactaatc attctggtca cttctagcga
60acaacatgag acagaatgcg atgataattt cttcagatgt cgctcaggca gatgtatatc
120tatgagcttc atttgcgatg gatatgaaga ttgcggccat ggggacatgt
cggatgaaga 180gaattgtcat atgaccacta ccccaggaac gaaaccatgt
aataaggatg aataccagtg
240ccaagatagg ctatgcattc cttcgttatg ggtctgcgac ggtgaagcgg
actgtttgga 300tggcagcgat gagactcatg gatgtactgc ggggacatgt
gacggttttc tgtgccacag 360caagacttgt atccccaaag agtgggaatg
cgatggcgta agggattgtc ctgataattc 420agacgaattg ttctgtacta
agaaaccagt gaactcagaa gaatgcagta ttcatgaaaa 480tggtttcttg
tgtcgtgatc aaatgaaatg tattgaattg aaagaagtct gcaccctgat
540tgtgatgata gttctgatga aggtggcgtg tgcctcgttt caaacaaaac
agatacatgt 600actaatctga aatgttctac taaccaaggc tgcataatat
tacctgaggg gcctgtatgt 660actgaggtgt gcatgaaagg ataccacttc
actggtggtt cttgtttcga tatagatgaa 720tgtttatcat ttggaagttg
tgaccaaata tgtatgaaca caattggagg ctacaattgc 780tcctgtattg
aaggctataa atttaatatt gatttgaaca aatgcaaagc taaaggaagt
840gaaggcatat tatttttcac ttcaaaccaa gaaataagag gatattattt
agactctgaa 900gtctacttta aagtagtaga aaatctacca catgcaacag
gagtaacata tgatggtgtt 960aatgtttatt ggacaacagt agcagatgag
gaagaaacaa ttgtcaaagc agctgaaaat 1020ggtgaaaatc aaaaaataat
tgtaacttct ggtattggca gacctgaaga tctggcagtt 1080gattggatta
caaaaaatgt atacttcaca gattctaaat taaagtatgt aggtgtttgt
1140tccaatgatg gatactattg ccttatgctc cacgaagatc aagtggaaaa
accacgtgct 1200attgtcctgc attcatcaga aggattgatg tattggtcag
attggggaac agaacctgcc 1260attatgcgtt caatataata aaatttataa
aagatgacat tcattggcca aatggtctag 1320ccatcgacca tggaacaaat
tatttatatt gggtagatgc taaagaagca agaattgaat 1380gtgtaaaact
agatggaacc gacagaagaa aagtaccatc cactgctgta aaacatccct
1440tttccattga tatttttgaa gatagaatct tctggagtga ctgggacagt
gaccaaatcg 1500tatcctgcaa taaattttct ggcaaagatt gccatacaat
aataaaagaa agagaccata 1560agatttatgg tatccatgtc taccatccta
gtaaaggaag ttattaatcc ttgccttgat 1620gttcaatgct ctgatatatg
cctaatttct ccgaaaaata tggatgatga tttgcaatat 1680acttgtgctt
gtccttatca taaaaaactt agcagagatc atcatacatg tattgaggat
1740aagccacttc aaaccataat tgtgggttct ggacagcaga tcattaaaat
acaacataaa 1800aaatttggag gatttagttc agatcatttc aaactaacag
tattaaggaa aataggtgct 1860ctagcttatg atccaaattc agatagaatc
attgtaagtg acttagttag acgtaaaata 1920ttttcattca acgttaaaag
tttcgaatca gagatattaa ttgaacacaa tattggaaaa 1980gttgttggta
tggatgttga ctattatgaa aataatctat attggataga tgatgagaaa
2040aaaactattg aagttatgaa tttgcaaaac aaacatcgat taacactcat
acgagatcta 2100aatgaaggtc tcaatgatat agcattggtg tttatgtttg
ttgcactttc aacatttgag 2160ggtgcacaca ttgacaggat atcaatggat
gggcgacaaa cttccagaat tcatgtaatt 2220gaagagaaac tatttggccc
tttgtctctt agctatgatc ctaaacttga gcgactgttc 2280tggtctgatc
aaatgggagg tgaaatagct tccacagctg ttgaaggact tgatcgacat
2340atttttaaag acggacattc cggtccagta gatgttgcag ttactgagtc
agaagtattt 2400tggcttggtt atggagctac gaaggttttt tggtcaaata
agtttgatgg atctcttaca 2460aaaagattta ttttagacaa gcttgaagaa
actgataaca tgaaaatcat tggattaaat 2520aaaggtttaa ggaaggaagc
aagtgcttgc aatgcccttg gaaactgcag tcatgtctgt 2580ttgatttcta
cacatatggg gatttgtgca tgccctgacg gaatggagct tggatttgat
2640agaaaaacct gccaaaaatt acttgcctgt aaagaaggac aatacaaatg
taccactggt 2700gaatgtattt caaagtctct acgatgtaat ggtagatcag
attgtaggct tggtgatgat 2760gaagaatatt gttctataat ttgttcacca
gaccagcttg cttgcttaga tggttctagt 2820tgtattgaaa aatctaaaaa
atgtgattct aaagttgact gcaatgacgg ttctgatgaa 2880aaatattgtg
aatctactcg gaattgtact ggaaatgaat tccaatgcac atcaggagaa
2940tgtgtttcca aagtagcaca atgtgatggc atgaatgact gcaacgatgg
cagtgatgaa 3000agcagttgta atgctttcac ttgtaaaact aatgagttcc
gctgcaatac aggaaactgt 3060ataccaaatt catgggtatg tgacagtcag
attgactgta atgatggttc tgatgaaggt 3120ccaaaatgtt ctgaaattga
aggatgcgat gaagatcagt ttacttgtga caatggtcac 3180tgtatttctc
atgttttggt ctgtaaccat gaagatgatt gtgaagattc atctgatgaa
3240aaatattgtt actttccaaa aagcaaaaaa aaatatgata atgaggaaaa
taagactcag 3300gattcatcaa aagactgcca atttttatgc ccttcagatt
taaaaatatg tttgccttca 3360tcagggagat gtaatggaac ttcagagtgt
ccagacggag aagatgagct tcgttgcaac 3420aagtgctctg aggaagaatt
cacttgttct tcgagtaagc gatgtttgcc aatgacatgg 3480gtttgtgata
atgtcagtga ttgtgaagac cagtctgatg aaatgcattg ccttaatgga
3540accttggctg tacacgatat gtttatttca gacaatcttc tatgtgatgg
tttattctgc 3600aaagatagta agaaatgtat tcccttaagt aaactgtgtg
attctcttat ggattgtact 3660gatgggtcag atgaaaaagg cctttgcggt
aagggatgtg aacacgctgc atgtcatcac 3720aaatgtaaag agacacctat
gggacctagg tgtgaatgct taagtggtta tactctagga 3780ggtgatggta
ggacttgcag tgacatcaac gaatgtgaga aaatgtcttc ttgctctcaa
3840tattgccata atactgttgg cagttatagt tgttcatgca tggatgataa
ttttcaacta 3900agatctgata aaataagatg caaagcaaaa ggaactgaaa
tgaagtatta ctttgccacg 3960aaaaaagaaa ttaaatataa atcccaatcc
ttacagcaaa cagttgtagt gtatcaatca 4020cagcgtgata tggatatgaa
aggaatggat tttgatatga ataatgatat aatattttgg 4080acttcagaga
ttagtggtta tttgtacaaa gttgatatta aatctaaaaa catcactcaa
4140attacaaacc tcataagacc aacaaaaata tcatatgact gggttactgg
taatgtttat 4200gtccttgaaa acttcaaaat tataagagtt tgcaattttg
aagctaaact ttgttcagca 4260ttgtacacag ctaagaatgg tatcaacatt
gaaaccattg ctcttgatcc aaaatcgaga 4320atcatgttct ggtctgaaag
taaatggtta ataaatcaaa cacccaatag tacacttcag 4380ctggcatcaa
tgagtgcaga taatccttgg gcagtgctaa gttttgattt tagtccagtt
4440actgatattg tagttgatca ttttcacaaa gttgtttatt ggtcaaactt
agcagaaaat 4500aaaattgaaa gatgtagtta tgatggaaaa gacagatctg
tattattgcg tagtgagtat 4560cctccaagag acttggtact gtttgaagat
tatttatact gggtaaatga tcaaaaacaa 4620tctcacatta ttcgtccaga
agcagaagga actattacta aatatgggct gtatggatct 4680gtgtatatga
agcatgaatc agtaaggctt ttaccttctt catcctacaa tgaaattaaa
4740gctttccggg tttcacagtc tgcactccag cctgaaggag tgaatcattg
tagcaaaatc 4800acttgtaatt acttgtgcat aagcaatgaa aacgaaccaa
tatgttactg tgaagatggc 4860tcaaaagtga aacccggaga tatatgcaac
aatcatactg gaatacattt caaaacctat 4920gaaacagaaa atgtatcagg
gagtagttca gtattagctt ggtttttctg gattggtggg 4980ttagttatag
cagctggact tggttatgca agttatcatt ttgtagctaa ctatgttcaa
5040aaaaataaaa tacacccaag gttcagtgta ttttttcctg ttggccgttc
tgaggctcag 5100agagaacaag gatcacaggt tcatcagttt gaaaacccta
catatagtga ggtcattgtg 5160gtataatgaa atatgaagtt aattta
5186363378DNADiabrotica virgifera 36agaatgacgg attgaattga
aaaagaaaca gctgaacaag aattaactgt ctacgaatga 60tgaaagtaaa ttgatgaagt
ataaaactta gaaattagat aacagaagta gtcaactgca 120tccaaattca
gctgatttag ggcatttacg ggaacctttt accttttggg aagtgaaagt
180ggccgtgata tcgaattagt ataaccgaac tgaaaatgac ccatgtagta
caaacacaaa 240aacaagtcat tcgttaattg gtaaaagtgc ctaaggtcct
attttcatct tgtaactatg 300aaggattgtt taaagtgttg ataagttagt
aatgtaccct aaaattaatt gtgtgtaatg 360aatacctgct agtaattacc
gaatttggag cgagaaaaga gaaagaaaat gtcatcgata 420aaaagtggag
gtctaaccaa aaaggaaggc aagatgagga tcagagcctt cccaatgact
480atggatgaaa aatatgtaga aagtatatgg gctttattaa agaatgctat
acaagaaatt 540caaaagaaaa ataactcagg tttaagtttt gaagaattat
atcgaaatgc atacaccatg 600gtattacaca aacatggtga acgcctttat
acaggattaa aggaagtggt cacccaacat 660ttagaagtga aggttagaga
agatgtgtta cgatctttac ataataattt ccttatgact 720ttgaatcaag
cttggaatga tcatcagacc tccatggtta tgattagaga tatactcatg
780tatatggaca gggtctatgt ccaacaaaac gatgttgata atgtatataa
tttgggactt 840atcattttta gagatcaggt ggtacgttat ggctgtatta
gagatcatct tcgagagact 900ttattagata tggtaatgcg agaacgtaga
ggagaaaaag ttgatagaat ttcaattaaa 960aatgcgtgtc aaatgttaat
ggtacttgga atcaactccc gagttgtcta tgaagaagac 1020tttgaacggc
cttttttgca acagtctgca gaattttata aggtggaaag tcaacggttt
1080ctggctgaaa atagcgcatc agtgtatata aacaaagtag aagcgcgaat
caatgaggaa 1140tccgatcgtg cgaaacacta tttagataaa tcaacagaat
ctcgaattgt tgaagtagta 1200gaagaagaac tgatcaaaaa acacatgaag
actatcgtag agatggaaaa ctctggtgtg 1260gtacacatgc tgaagcacca
aaagaccgaa gatctatgct gtatgtacaa actgttcggt 1320cgcgtaacag
atggtttgaa aacaatggca gattgcgtca gtttgtatct tagagaacaa
1380ggtaaagccc tcgtccagga agaagaacac cagccggcca ccaatgctat
ctccttcgtg 1440cagtcattgc tcgatctcaa agacggcttc gaccatttcc
tccagaattc ctttaacaat 1500gataaaatat ttaaacagat gatagcgtct
gattttgaac atttcctcaa tttgaatccc 1560aaatcgccgg agtatttgtc
gctgtttatt gatgataagc tcaagaaagg tgtaaaaggc 1620atgaaatctt
ctaaccttga aagattcctc aatttgagcc ccacatcgtc agaatatttg
1680acgctgttta gtgacgatta cctcaagaaa ggtgtagaaa tcatgaccga
acaagagata 1740gaacaagtat tggataagtc aatggtttta tttaggttcc
tccaagagaa agacgttttc 1800gaaaggtatt acaagcagca tcttgccaaa
cggttgttgt tgaataaatc tgtcagtgat 1860gattatgaaa agaatatgat
ctctaagtta aagacggagt gtggatgcca atttacttct 1920aagttagaag
gaatgtttaa agatatgact gtttccaata cgattatgga tgaatttaaa
1980gaacatgtta cgaagtctga gacaaattta ggtggaatag atttattaat
gcgagtgctg 2040acaactggct tttggcccac acaaaacgca accccaaaat
gtcatatacc agctgtacca 2100ctagctgcct tcgaatgttt taggagattt
tatttagcca aacacagcgg ccggcaactt 2160acgttgcaac ctcaacttgg
aagtgccgac ttgaacgcta tcttctacgg cccgaagaaa 2220gacgatagcg
acaaggatgg cgcttgttct tcctcgacaa caatagtctc gatgaggtca
2280gggcctagga agcacatcat acaagtgtcc acttatcaaa tggtagtttt
gatgttgttt 2340aataatcacg ataagttgac atatgaagag atccttaatg
agtctgatat accagaaagg 2400gatctaatta gggcgttaca gtcgctggca
atgggtaaag caacgcaaag agttctcata 2460aaaaatccca ggaataaaga
aatagaatct aatcatgaat tctatgttaa cgaatcgttt 2520agttctaaac
ttcatcgagt gaagattcaa accgtagctg caaagggaga gaacgagccc
2580gaaagacgag aaactaggaa caaggtcgac gaagatagaa aacacgaaat
tgaggcagct 2640atcgttcgga taatgaaatc gcgtaaacgc atggcgcaca
acatcctcgt cacagaagtg 2700acagaacaac tcaagagccg tttccttcct
tctcccgtca taataaagaa aaggatagaa 2760ggtctcatag agagggagta
tctggcccgt acgcccgaag acagaaaagt gtacacatac 2820gtggcatgaa
ctttaacaag gatcattatg agactttgga tagtggtcca gtgatcttca
2880atagatgaat atttacataa atgtaattat taattattaa attattccaa
caggttcttt 2940ttgtctaatt tcttcaagtt gttgaagaaa aataatgtat
ttgtggaaag ataacttccg 3000gaccatatta acaattttca atttatttat
aataattttg tgatagattg attgacaaga 3060taattttaga ttttcagttt
gcaataaata atgtagagtg tgtttgatga tatattaaat 3120ctcgatttgg
acttttcgga aaattaacac gttgatggac agaacgtata ggcgtctaat
3180ttccattgat gtaatgtgac acaacgtcta ttataatttt taaaataacg
agttgtaaca 3240aaaaatccat gtcagaaaat atcacatttc atctacagag
gcacattgtt tccataaatt 3300aaaagcgtta aaagaaattc agcaattttc
ctattctact ttgcaaaata caaggtgatt 3360cagtgaaacg gtacgatt
3378371786DNADiabrotica virgifera 37caccggctta cgtaataatt
aatttattcc tttcatttgc accatactat atattattta 60ttgagtggac aaatgttaaa
aagtaagtct ggcagcgaac gtgttaatga aacctagtaa 120aaggctccaa
catttgacac ttcttcatgc aacatgttct gttctgtgtc aatctaacct
180aacctaacat tttcaaatac gaatatggaa acatttcacc acatcaacac
gtgaattaat 240attcgaaaat ggagtacgaa aatacacaac aaaatataaa
cttcgtaccg tgtgtaagat 300gggttaaacg aggagtggcc aattcaagcc
cagtaaaatt gcaactgtcg aaaaacgagc 360tggctcaaat tattaatgac
accaagatta aattacaaga atccaatgaa aatgaagatg 420agcctatgga
agaaggtgaa acgtctcaaa cagatgagtt tgccttagag gattacgata
480aagaagacga aaatgaggac actgcaaatg ctttaggaat tggatcattg
gcagaactcg 540ataatgatgc tgcagacaat ttttctgagt cagacgattc
tgaaaaagaa gatgataaaa 600tcaaaccatc tgacaatctc atactagtag
gacatgtaga aggggatgca agtctattgg 660aagtctacat atacaatgaa
caagaagagt cattgtatgt tcatcatgat attatgttat 720catcctttcc
tctgtgttta gaaccgctaa actatgaacc gaagatgccc aaaggaaatt
780attgtgcagt gggatcaatg tcacctgtta tagaggtctg ggatgtagat
attatgaatg 840ttattgaacc ttcatttact ttagggagac ctgctagtaa
aaagaagaac aaggaacata 900taggccatac ggatgcagtg ctttcattag
cttggaacaa aacctttgaa catgtgttgg 960ctagtggatc tgtagatcaa
acaatacact tgtgggatat ggaaatcaaa aaaccaagta 1020caaccattaa
atccttccaa gaaaaagttc aatgtctcga atggcatcct ttagaggcac
1080aaacactttt aggtggaggt tgtgacaaat ctgcaagagt atttgatggc
aggacccctg 1140aaacccacca aacttggcta cttgatggag aagctgaaag
gctatgctgg aacccattag 1200aacctttcac atttttagca ggtaccagca
gtgggtctgt acagtgtttc gactgtagaa 1260aaggacagct atggtcagtt
aaagcacaca gcaaggaagt aacagggtta gtccttagca 1320aacaatgcca
aggattgttg attacttctt ctacagatga aacagttaaa atttgggact
1380tggctacact ggaagctgaa cctaagcttg ttaatgaaaa ggagtttaat
atggggaata 1440ttcactgttt ggatttatgt ccagacttgc cgtttgttat
atctgtaggt ggagataaga 1500agtcaaataa ttttactgtt tttgatgtac
agaatattga tgttgttaaa cacacatttg 1560ggccaagagg attggtacag
ctagttccag atacagaaga aaatactaat acgttttatt 1620gtttaataaa
gtttttattt tatttatttg taataatttc atgcttcttc tttaagtacc
1680gtgcccaaat attagtcatt ggtagattcc ttgacaattt gccgatactg
ttttcgatta 1740ctgaacaatt catcttctga tgagccagtc cattgacaaa ggtttc
1786383948DNADiabrotica virgiferamisc_feature(3773)..(3795)n is a,
c, g, or t 38tggttcttac caacttccag acattttttt ttggtggtta aggcccatct
aaaatgccca 60aatcaaataa atctagaaat gcgttttact actttatgga ggattttaaa
caaagacaag 120gatttgcttg taaatcgatg aaggatgtag cagatgctgc
aggtccccat tgggctagga 180tgtctaaagc agaaagaaga ccttatgaag
agagggcaaa caaagagaag ggaggtttga 240gatatacttg ggatggtcaa
tgcgttgaag atatagaaag gaggaacaaa gctgaggaca 300ataaaattgc
taagatgaaa gatgatatcg actgcacctt gaaatttgcc aaagaaaatt
360gtcgactccg agatgaaatg ttcttcataa tccatatcaa tactttctgt
taccatgcca 420gtggtgatcg gtattatccc gctgaaattg ccatttcatg
cttcagcttg gaggatggcg 480ttttgcctca aaatgtgttt cataaaatta
ttaagccagg tagactacca ctggggtatg 540ctagtgacgc aaaaaagcaa
tctgaagatt cacatcaact gcctatcccc ttaactggtg 600acgacgacga
caatgtggaa gaagtttata cagagatgaa agaatttata ttatctaaaa
660ccggagatac taagaaattc cctcccttgt atgctaagaa agatattgtt
aaaatgttgc 720aacatgtgtt agacacttgg tgcgctgatt ttgatgagcc
gccattattt aaagtttatt 780atttacaata tatgtttcaa gtattaaaaa
actcggtagc taacgataac gtatggccct 840tatattcaat ggcagaaacg
gaactggata aggatcttta ttcccatact gttggaatat 900gttgtgaatt
ccattctgtt agtgaagctt gtcaattttg cagcaagtca gtggcagtac
960gacttgcata cacaatttgt gataattgct gttcgttcct agatattcct
atagagcctg 1020gaagacacgt acctgatcaa gcttttacag catccagttt
gagctcaaga gcttcatcaa 1080aagctagttt gtatacaggt tccagtaaat
cttctaagaa gcagtcagtt cgtgaagatg 1140acaaagacac tgttttatcg
ttttctagtg cctctcagtg ggagagcgaa tctgtagtgt 1200cagactcctc
tacagctact ttcaataata gctttccagg tctgaaatcg aaatggacat
1260taagcaattc tcaacctgta gaaactgact caatgagcca aatgtcaggt
cagagctatg 1320caagtgctat gggaactaga agacagtttt ctagtgcaat
gggaaataga ggatctcaaa 1380gagagtcctc taacaattcc aacaatggtg
acactagtga ttcgaatacc gattatttta 1440acagtcaatc atttccggct
cttggaaggg gaagaggtat ggccagaaat cgaagaaatt 1500gatcattttt
gactgcaatg ggtcttgttc cacttcatga gattgttaaa tgagtatgta
1560actgattaca ttaggcgaca atattttttt tagtgtcaga ttatttgatg
taattgtcct 1620tttgacaatt ctttaatcat tgatgttttc ttctattgtg
gtgtttttag atgtcatatt 1680tttatgtatg taaattggtt ttttatctaa
gtatagaata ttacatttta gtttaataag 1740ataccagtaa agtatattgg
tttaccagtc gcctaatgta aactagtata tacataccta 1800tattattttt
ctttaatagt tttaagtcaa ttttgataca gatcacgaga cagatatttt
1860ttccgtctag tccgaattgc gttccgtttc ttgggccttg tcgaattgcg
tcccaaatat 1920tttgcttacc tggccaccga taacgtctag tccgaattgc
gtttcatttc ttaattagcc 1980aacccaattg cggtcagaag atagcttaat
aaatattagt taagcaggga aatacaatca 2040acgtaatatc aactaacagt
ttatttttat tttagcaccg aatttataac taacacattt 2100aacgaaattg
tatcttgaaa tttcacccct gttatactgg ttaattttct gttcgagaaa
2160aatatatttg ctggaggtag ctttatctac atttctagaa atattttgtt
taacagaatt 2220tatttttata taagaatcgg tttgaatgcg ctttaatgtt
tcaacaaaca aaaaaatatt 2280aggatgtgga gacaaacaat aatttttaaa
ttttgaatga aatcactcac aagaattagt 2340tgttctatat atagatgcgc
tattctttgc ccaaatttgt ggaggaaatg tgtagtcctc 2400ttcgatatac
ctttcaacta gatagtttgc aaatgcgtca actcgatgat catctggttt
2460ttcaggcata aaatcttcca caaaacaatc tccaacttgg ctcggaggaa
gatacattag 2520gccaaaaata tgcttcagcc atttacctat ttctgagttc
tcgttcttat actcagaact 2580caaccccata ttttgtactt tacgccacct
gtaacaagat tatagaataa attctttatt 2640tcagaaagta cttgcattca
agaaaaagtt tgaaacaggt cagattttat aaaaaaaata 2700catctttaaa
aaataatgag aatatttagg agccaatttt ttgacagagg ttatgacgac
2760atcttttttt tatgtcaaaa atttgaagat attttaccta caattcttgt
tttaacttat 2820atcaaatgct gttttttgta aaaaaaaatt gaaaaaagaa
attacagtga agtttgaaat 2880aaacatattg aaatgtttag ttttacagca
aaaaaaatag gatgacgtca taactcaagt 2940ttttcttaac aatctactat
ttgccagtta cattgccata cgtattactt accaagcttg 3000agcaagatga
agtcgataac caacaacatt tgcttccgga aaaacttctc gtacgctgga
3060gtggatacct aattcgtaat caatcactat tttcgttgga tttaaattgc
atcccaaatc 3120ttgacatttt ttctttaata attcgaataa tgatttgtaa
gtgaattttt gcttattagg 3180taacaaacaa aatgcaaccg gaacataagt
gtcattaatc aatgtatgta atgtaaacat 3240ttaaaaaaaa attgtgcaat
aatcgaatgt tccatccata aataaagtgg aattgttaca 3300cataaatgaa
agattagaca ttcatgaaaa aacaaaaata ttaaactgtt catcattaat
3360caacaaaaac tgttcatttt tgatagtaaa tatttcaatt tgttccaaaa
attcttgaac 3420atccttggct gattttggca attttggaac cgttttcaat
cgcgtgcgat taatgttctt 3480tttttatgta ttggacatct tttgttgaca
gagaggtaag tacttcgctt tgcttttcga 3540tttccttgtg taacaacttc
gcaggtcgtt ccgcaatatc ttctgttgct tttcttttaa 3600gagaattgtt
aactttctgg cgaattaata cttcttcagc aagctcatga ttgtgttcac
3660cactaacttt tgaaaacaca ttatttaatg tatatagttt tgctttacac
tttttgaagt 3720tatacttctt taggcgcgat tgagtaatta tttattatta
atctgcgcgc atnnnnnnnn 3780nnnnnnnnnn nnnnnggttg ctaatctttc
aaattatgta tcagcgcaaa taagccatta 3840aaataatata ttagtgtttt
ttagtaaatg tattatttat tatcattttt gtgtctttgg 3900atttgttttc
ctttggcgta aaataataaa atatctattt ttatattt 3948392088DNADiabrotica
virgifera 39caatcaagtg tcaaattgtt tttaaaaaat aatgacagag gaatcagtta
cagcaatttc 60cgaacgagaa gaactagaac cagaacttcc cccaccacca ccgataactc
tgaaggaagt 120ctttgtacaa gaacatgaag atagtcctga ttcgtctatt
tccgggtccg atgaagaaag 180aaaaacagac aaacccagag ctgatatacc
tcccgaatac tggcacatac aaaaattgat 240taaatattta aaaactggca
atcaaacggc tacagtggtt gcattatgtt gtttaagaga 300ccatgactta
actacagaaa ccagtcagct tgcaatccaa gaaataggcg gcctggaaat
360attaataaac ctgctagaga caaaagacct caaatgcaaa ctaggtgcct
tgggcgtttt 420atcagagctg tctacaaata tagatatacg caaatccata
gccgatttgg gtggagtgga 480gttattggta cagaatttag ctgagccagc
tagagatctt caagtacttg tagccgaaac 540tatatacaat gttgctcaaa
tccgaaaagc taggaaacac attcgaaagt gtaatggaat 600acctaggttg
gtcgactttt tagatgtgaa ggagtctcta ttgaagacgc cgaaagatca
660gttgaatgag tatgatgctg aacaggtaaa catagcaaaa gctgccacca
gagccttatg 720gtccgtttct aaaagtaaga agaatattcg
cgtaatgatg aaaagtggtt ccgtgcccct 780actagccaga ctgcttaaat
cagaccatat ggatgtggtg gtaccaacta tcggaacgat 840ctcccaatgt
gccatcgatc ccacttatca attggccatc cagactgaag gaatgattaa
900ggatatagtt ttgcatctat tttctgagga atatccagct ctcagaagat
actgctcaga 960aactatattt agatgtggag aaaatgctct gattagagat
atggtaagag aagctggtgg 1020tctggatcct ttgatcaaga tggtaaggga
tccgaaaaca aaggaagaca aaccgctatt 1080ggcggcagtg actggagcta
tctggaaact ggctataagt ccgcagaatg tagaacgctt 1140cgatcagtta
aaaacggttg agagcctagt aacattattg gaaaacgtcg aggaagacga
1200gggcgttctt agcaacgttg ttggtgctct tggagaatgc ctaaagtttg
aacacaatcg 1260acacaacctg cttagagcta atggtatacc tcatttagta
aatctgctaa attataccta 1320tccccctctt ctcgaaaacg ttccaatggt
tttaagggaa tgcgctgaag attatgactc 1380gatgcgtgtt atagaagaac
tggacggagt aaggctgatt tggtcattgt taagaaatga 1440ctcgcagaag
gttcaagcta acgcggcttg gtcccttgtt ccttgtgtca gatacgccac
1500agactcagga gaaatggttc gctgcttcgt tggaggatta gaactcatcg
tgaatctatt 1560gaaatccaac gataaccacg ttctagcatg cgtgtgtgcg
gctgtttctg aggtagctaa 1620agacatcgaa aatctggcag ttatgaccga
ccatggggta gttccgctat tggttaatct 1680ggtgaataca gatgatgtgg
agttgagaca gcatttggct tcagctattg cttattgctg 1740tgcgtgggga
accaactgca aaatgtttgg aagattgggt gccattactc ctctagttca
1800atacatggcc gacgatgacg ccaatgtcca cagaaccaca gctctagcct
tgttccacct 1860ttctaagaat ccgttcaatt gcattacgat gcacgaaagc
ggagtagtga cttttcttct 1920aagagcagtg tcttccaaag actgggagct
acaagaagct gctgcggggt gccttgctaa 1980tatcaggaaa ttggcattgg
aagctgaaac tgtgcatctt attagaaata gggacgacac 2040ttctgatgag
gacaattagg gatacgcttt ttgtggattt ttatatac 2088402198DNADiabrotica
virgifera 40cgaactaagc taaaattaat cgtgaagtga aatccatttt aagaaaaaaa
tggtttaata 60agaataattt atacaaaagt gatatttata tattttcgtt gacgcgttga
ggatattgtg 120aaatatatca aatagtgtca acatttgaac aaatgactat
atatgtgtaa aagactgtag 180tgatcaccag atccaaaaaa gggtgtcttc
aagaaatgtg ttaaacccat gaatctttag 240aaaagagtta aattgaagat
atgagtggcc gaccaagaac tacttctttt gcagagggca 300acaagcaagc
taagaatcca cctttggggg gtatgaaaat cagcaataaa atttaaaatg
360gcgtcgaaaa gtaaaagagg aaaaagtaaa gaaatggaag atgatattag
taaacttgta 420tctacactca aagttaggcc aggtaaggat ggaagcaaag
tgactacagt agttgcgacg 480cccggccaag gaccagacag acctcaggag
gtatcataca ctgacaccaa agttatcggc 540aatggcagct tcggtgtggt
ctatcaggcg aaattgtgcg aaacttcaga attggtagcg 600attaagaaag
tactgcagga caaacggttt aagaatcggg agttacaaat catgcgaaaa
660ttagaacatt gtaatatagt gaaacttaaa tatttctttt actctagtgg
agataagaaa 720gacgaggtgt acctgaattt ggttttggaa tacatccctg
agacggtata taaagttgca 780cgacactaca gcaaatcgaa gcaaactata
ccaattagct tcatcaagtt atacatgtac 840cagttgtttc ggtcactagc
atacattcac tctttaggga tatgccatag ggacataaaa 900ccgcaaaact
tgttgttaga tccagagact ggagtgttaa aattgtgtga tttcggaagt
960gcaaaacacc tcgtcaaagg tgaacctaac gtagcttata tttgcagtag
atattatagg 1020gcaccggagt taatttttgg tgctatcgat tacaccacga
aaattgacgt gtggagtgct 1080ggctgtgttt tagcagagct tttattaggt
ctgcccatat ttcctggtga ttcaggcgta 1140gaccaattag tcgagattat
caaggtgttg ggaacaccta ccaaagaaca gatcaaggaa 1200atgaatccca
actacaccga gttcaagttt cctcaaatca aatcacaccc ttggcaacag
1260gtattcagag ctagaacacc tcctgaagca attgaactag tagcaagatt
attagagtat 1320accccttctt ctagaattag tccgttacag gcttgtgcac
attctttttt caatgaactt 1380agagaaccaa atactcgact tccgaatgga
aacaagctac cacctttatt taattttacc 1440gaacaagagt taagcattca
accagcattg aacagtatat tgttgccgag aggaacgcag 1500gaggcagcta
atcctcagga gagtacgtca gctgctagtc aggaagcctc cgaggcgaca
1560gcaactatcc aagcagcggc tgcttctggt atagcttagt taatagttag
tttattcgtg 1620ctagaatgtt taaatttaca cttgtcaaat ataataccta
aaatacttca ctctactgta 1680ggggtcgacc catttatgtt tatttgtttg
tcgatttaga taatttcaag gctgatgtat 1740cactttatag gtctattggc
caccatttga tatgttttag tttaaataat cgatatacac 1800atatggcttg
gtttgaaaac acctatataa tctttatata cagaagcata tatagtatat
1860atagtttaag tcaaatttat cactgtaaaa ttcttgtaat tggggcaatc
tagttaaata 1920aatttatcaa gttagttaat gtatttgtaa ttctttagat
tcagtatata ggccagcagt 1980ttacctcgca aaagatgcct agcaataaaa
tacagtgata ctactaataa ctgacaaaat 2040aaagcaaaag atggaaaaca
taaaagttgt gagataaaaa gaaaagaaac tagcagagat 2100ggtaaattta
gttataaaaa cctataaact tgaattctac ttaatgtttc ccaccttaga
2160catatcagag ctatatgtca actaaaactg tcactgtt
2198413910DNADiabrotica virgifera 41tttagcaaca tttacacggc
ggcggtagtt aggaagcaag cgctcgaagc gtcgcaatat 60agaacgtcaa taacgacgac
gacggatagc tagtgcaaca ggtgattccg cgtatttctg 120ttagttagca
gcgagcgaga aattacacaa cgcggtgaaa agggaaaagg ggaggtcgca
180acacaacgtg ttaatacttt tccttagaag attcgaacga ttcgttttta
gtgccgtttc 240acttcaaaaa tgttttagga aaaaaagtgc gtttaagtgt
catgtgtgca gtttattgtt 300gaatgtcccc ttacagtaga taccggcctt
taccaggccc tgatggattg gttctgcaaa 360catcacccgg gtgtagacct
tcaccgacca gtgcttgcag atttttgcgg tggatatcca 420aattgagtcc
ccagcccgtt aataaacaag gattaggggt cctggacatg accgccacgg
480agaacaccat ccgttttagt gtccataccc tggacaaagc aaccaaagca
aaagttactt 540tagaaaatta ttacagtaat cttatagccc aacatgtaga
acgaaagcag aggttagcaa 600aattagaaga atccttaaaa gacgacagtc
tctctgagga gcagaaacat gaaaagaggc 660tacaacatgc ccaaaaagaa
accgaatttc ttaggttaaa acgatctcga ctaggcgttg 720aagacttcga
acccctcaaa gtgataggta gaggggcctt cggagaagtt agattagtgc
780agaagaaaga tactggccac gtttatgcga tgaaaattct caggaaagca
gacatgttag 840aaaaagaaca ggtggcgcat gtgagagcag aacgggatat
tttagtagaa gccgatcatc 900agtgggtagt aaaaatgtat tatagtttcc
aagatcccat taatctatat ttaataatgg 960aatttctacc gggaggtgat
atgatgaccc tactcatgaa aaaagacacc ctttctgaag 1020atagtacgca
gttttatata acagaaacag cactagccat tgattcgatt cataaactcg
1080gtttcattca tagagatatc aaaccggata atttattgtt agacgccaaa
ggacatttga 1140agttgtctga tttcggatta tgcactggtt taaagaaatc
ccatcggacg gatttttata 1200gggacttaag tcaagcaaag ccttcggatt
ttattattac ctcaagtccg atggatagta 1260aacgcagagc ggaaagctgg
aagaaaaata ggagagccct ggcctatagt acagtaggca 1320cacctgatta
catagcacca gaagtattca tgcaaacggg atacggacct gcttgcgatt
1380ggtggtcact aggtgttatt atgtatgaaa tgttgatagg ttatcctccg
ttctgttcgg 1440aaaaccctca agatacatac acaaaagtga tgaattggag
ggatacgttg gttttccccg 1500ctgaagtacc gatctctgaa gaggccaaag
atacaatcat taaattctgt tgtgaagcag 1560acagaagact gggatctcaa
aaaggtctag aagacctaaa attaaatcag tttttccgag 1620gcgtcgattg
ggagcatata agggaaaggc cagcagccat accagtagaa gttaagtcaa
1680ttgatgacac atcaaacttt gatgattttc ctgatgttaa acttgaaatt
ccttctgcgc 1740cccttcctca agacggcgag atcaattata aagattgggt
cttcatcaac tacacattca 1800aaagattcga gggactcacc cagagaggta
ctccaacgaa gaaatagttg tttgttaata 1860gtttttacca cgataaagat
atactaggca gttgaatgcc cagcacttat ttttagaaaa 1920taaagtcatt
taagagatgt ttatattaat tatattaaat aaaaagtagt aagtttcggt
1980aaagacaaat tccacaatat ctttacatgc catcgatcat tccgtttatt
attaattttt 2040atttctttat tatttgattt ttaaaactat gctatttgtt
tcatcgtgtt cttttaattt 2100ttaactattg caccatcaac tcaaacggtt
tatacaaggt tggaaatcca agacatgaat 2160gaatatacag tggataaatt
ttgccacact gcaagtgtag tctacttcta ctcatttctt 2220tctgataaag
tcactacagc ggttttaaag agtttggtgt catttgctct ataaataacc
2280aaatatgttt tgtaagttgt gttcgtatag tgaaatttaa cattattttt
cctatatcaa 2340taaactatat tctttctaat ctgctctaca tttgagacaa
ataatttttt ggtcatttta 2400ttgcaaattg gattcttatt aaatatctta
cttatgtttt tataaaacac gacataccgt 2460ttttgattac acgtaaatcc
tgaagtaata ctattaactg aaaaaaaaac tcgaaatcga 2520taaaatgcct
tgtattgtat ctgctttttt tatctccgta acattcttaa cgatgttcca
2580tttgaatcct tgattccagt tcttcacgtt ccaatactgt ttcaattttt
tatttactga 2640ttttaattta tcaattaaat tatctttaaa ttaactgctt
aatcaataat gtatattagg 2700tctctctaat ttaagattaa ataaaatata
ttagataaat agagtatatt actaaaattg 2760aaaatacaaa aggaatttct
tagcttcata gatatattat taataatttt aaaaaataca 2820ttaaacagtt
gaagttactg actcgttagc aaactaaata aaaataaaac atgtggaatt
2880tgtttgtgcc gaaatcgtta aatgttctat cgattgcgct gtattgtttt
ctaaaaataa 2940gctattttgt gtattatagg tctgaagcag ttttgcactg
ttttgctttt ttaagagctg 3000tgaaaactgt atcttgcatt gttctcgacg
ctatacttct agaagtatcg tacacttcaa 3060aattatttaa ttcattataa
gcacttttcc tgtacaagac ttacatcgtt tcgtcaaata 3120ttaaatatac
attacgctta cacgattaac aattttacat tttaacttat gtgatcagtt
3180atgttacaat ttttattttt tacaataata gatttttatt tcctattttt
taatgtaaag 3240atctgctttt tatatttaaa cattattaaa gatttttggt
tttatacttt cattctattt 3300aaatgagaaa tttgatgtga ttatcaaaat
gatcgaaatg gtaatttgaa aaaaactaat 3360atttttgatt cggattatta
caagagaact gacaacttta tcattaaaaa gagtacctaa 3420ataattgtac
tttctcgctt tttctcactc gttagaagca tagatttggt tttgtttatg
3480tttagtgtag tccatattcc tcactatctt ccatgactgt attgatacgc
gcctgtaggt 3540atttgaagtg ttccgactat catcactgta tcatctgcat
atctcagatt gttaactcgg 3600cttccgttaa ttttaacgct atatatattc
ttgaccactc aatgttttat tcattcctcc 3660gagttcatat tgaacaggat
tgcgaaagca cacaactctg acgaaatccc ttctgtgttt 3720ctatattttc
agttgaattt tcttcttttt gtactaattt tagagttctc gaagtctaga
3780gttttcctgt atcatttatc tgattacttt cacattgatt atcgtaaatg
agccataata 3840aataaatatc cacaaggaaa aagttttcct tgtatggggc
tgtgggtttt cctgtagttg 3900gctgtaatcc 3910422386DNADiabrotica
virgifera 42aaattttgac attgcatgat agcatgtgaa actatcatta gctcataatg
tttgtcagtt 60tttgactttg acaagagttg acaatctaaa aggtgatcaa agaaagattt
taataaaagg 120ttaaaaacac atttgttctt tgtaagcttt gttcttaaat
tttgttaaaa aatgtcagaa 180aatagagaag aaattttagc tgattttcaa
gcttgcactg gaattgaaga tgttgctgaa 240gctatttatc acctggatga
ggccaactgg gacttattgc gtgctgtgag tagagtaatg 300ccaccagata
ctcaaagttt tcgtccacca gctgtagagc ctgtagatcc agatgtcatg
360atagtagaag atgatgctga tataccaagt gcagtaactg cagacgcagc
atcagatatg 420gtagtagtag gcaacagttt gggccattcg tctacagtaa
cgaaaaaagc aaatacacct 480gatgacagct ttccatccac ttctgctgga
aaaagtggat cacgaatgtt aaaatttaat 540gtgcagtact gtgatagagt
tgtcaaagtt gaattaccag agtctgcaac cgttagtgat 600ataaacatta
atcttttaga ctgtaatgaa gaattcgtgg aagagtataa aagtatcatg
660aatgatctga aactaaagtt gcaccgagaa gtgaaagtaa atccttgcag
acagcttctt 720tccagttggg ctcgattagc acagtatgaa gatactccgt
tttcgaagct catgttacca 780aatgacaatt cgcttcattt aacagtaaaa
ccaagcgatg gagctttcac ctcagacgaa 840gaaaatgccg tatctgaaaa
attaagaggt acttacactc taaacgtaaa atatgagaat 900aagacattta
atttaaaata tcaaggaaca aaaactattt tggaagtaaa aggtgacgtt
960tacacgttaa cgaatataca tgtgcgtaat caagtatgga gtgggtggcc
tcctaatata 1020gacgacaata cagtactcgc actcagtgga ataaactatc
ctgaacatga tctatcagtg 1080cgacgaagca cacagagtgt tagagagaaa
aaaagtatgc ccgtggttca aattgacagt 1140gacgatgacg aatttgaaga
tgcctccgaa agttttacag ttgatgatga gtattttgtt 1200gacaatgaag
gagcaaatgc aaaaagaaca gacccattaa ttccagagga tgtagaagat
1260gaaattgtag gctgtataac atttgcagaa cagtttacga caagatacgg
tcctgtccat 1320cctgccttct atcagggaac attagacgat gctatcaaag
aagcatgctc aaaacctgcc 1380aaagaccgaa aaattttggc tatatactta
caccacgacg ctagtgttct tacaaatgtt 1440ttttgcacac aactgttagg
ttttgaaagt gtaatgcaaa tgttcgaaaa caactttgtc 1500ctttggggtt
gggatattac ttttgaaagt aatagacgca ggttgcagca atcggttacg
1560acatctttgg gtgctacagc agcaatgagt ttacgaaata taccagtaga
tagattacct 1620gtcattatac ttataacaaa aattcgatcg acaattgaga
tttttagtgt agtgtatgga 1680aatgttggtg taaatgagct tttgtcaagt
ctgatagaat gtgtagaagt atttttagaa 1740catcaacgag tagaaataaa
ggaagaggag gaaagagccg aaagagaaat ggttaaatta 1800gagcaagatt
tagcatatcg tgaaagttta gaagcagata gagcaaaaga agaggccaag
1860cggttacaag agaaaaaaga aactgaaacc aggaaacgac tggaaagtga
aaaggcactc 1920tcggaagcaa aaaaagaggc ctttaggaaa caagtcgaag
cagatttacc tcccgagcca 1980tctctagaac aaggcgacag tattactaaa
atacgattta ggttgccaaa aggagaacat 2040ttggaaaggc ggtttcagac
gactacacct ttgaaagtgc tgttagactt tttaatagtg 2100aaaggatatc
ctacggaaga attcaaagtt atatctagct ggcctaggag agacttgact
2160tcattagata aaagccaaac actgaaagaa ctgaagctat atcctcaaga
aactgtgata 2220ttagaagagc gatagttttg atcacacatt ccccaaccaa
gggttaggtt tttataccag 2280catttaagcg ttgtaaagtt atttaatata
tttttaattt cacgtagtgt atgattaatt 2340taaatgtatg cagtccgatg
aatatacatt acataaaaaa gaaaaa 2386432657DNADiabrotica virgifera
43atcgtctgtc gtgtttgtca ttttgttccc gcataaacct taacgttctt gttgtcatgg
60ttcatgtgtg atgaccgtta aaaatttgcc gggatatttt gcttaagttc ggccggaagt
120gattgattat ttatatattt tacaaacgat tatagttcta cataatggag
acaattcaaa 180acgaaaacaa ggagaatttc gtacatgaaa tgcaaaagca
aagcgccatg gccgttcctc 240aaaaacgacc attgggccag aggaatacca
acgtggatcc gcaaccagtt caattgaaag 300aaaaacaacc acttacagta
cccagttcat ttaatgcatt taaagaacaa tttgaaagga 360acaaatataa
aggaacaaaa aatacaaata aagaaaatta ttgccaaaaa ggaaataaga
420acatagttaa tccagttata ccctgcaacc agtttaaaga ctttaaagta
ttcgaagatg 480aacagtatcc ggaagaaaaa cttaaaaatc ttgcggctaa
aaatccaccc aaagtacaag 540tacacgaaga taccaaggaa gagacaaaag
atgaagtgaa agcgccagaa aagatagtag 600aaaaggaaaa tataaagcaa
gattcggaat cactcagtct aaagacatcc gatgcacatt 660tattcatgac
tatagatgat aattataaaa aagaccatga aattccgtca acaaaacgta
720tgaaagatat tttctttgaa atggaagaat acagatctga tatttatcac
tatctaagag 780aacatgagtt acgaaatcga ccaaaacctg gatatatgaa
gaaacaacca gacgtttcat 840ctaatatgag gacaatattg gtagattggt
tagtagaagt agctgaagag tacaaattac 900acacagaaac cctgtatctt
gcagtaaatt atatagaccg atttttgagc tatatgtccg 960ttgtaagggc
caagttgcaa ttggttggaa ctgcagcaat gtttatcgct tcgaaatacg
1020aggaaatata tccaccagag atcagcgaat ttgtatatat tactgacgac
acttacacga 1080aaaaacaagt actacgaatg gagcagttga ttttgaaagt
tttaagtttc gatctctcaa 1140tacctactcc actaacattt atcacagcaa
tgtgtgttag caacaatttg agtgataaaa 1200ctatgtatct tgccatgtat
ttgagcgagt tggcgttact taacggtgac atctacctgg 1260agttcttacc
atcagttata gccgccgcag caatagcgct tgcccgccat actttatgcg
1320aagaagcttg gcctaaagaa ctcgttcaat ctacaggcta cgaaggtctt
caaatcagat 1380ctggcatctc gtttttatac cagatgtttg tttccgctat
gactttacct caacacgcca 1440tacaagacaa atacaagacc aggaaatgta
tgcacgtatc tactttgaaa cctcgcgatg 1500tggcgttaga gttataattt
aattagtaat atttgtaata ggcttaagtc tgccaagggg 1560atcgaaaatt
ttatggctag tttaatcgaa aaaggttcca tgccccgact tcgaagaggc
1620aataaatgct cgaatgtcaa tggtgcgcgg atgcgctgtt gccaaatcta
cggaatgaaa 1680ttaaattttg ttctatcttg tattaataaa taaaacacgg
ttgcatatga catatatgaa 1740atgaaattac aaacaaaata gttgtagatt
tcggtccttg gcaaatgaca agtattgata 1800attaccgact gaatattatt
taatcaacat taataattta tcgtaaattt atttagttca 1860ataattacaa
gacatgatta aaagacgatt agaatcataa tcaaaccaaa ttacacttcg
1920tcaagaatag taatgtactt atcattgact tgacttcgtc aataatagta
aaggattttt 1980aataaattca tatgcctacc ccccttttaa acttttaaag
aaaatttaag ttcattccgt 2040aaacttggca acagcgcatc tgagcgcaat
tagcttttga gtattcattg cttcttctag 2100cttggggcat ggaacctttt
tggattaaaa ctgcaataaa attttccacc cctcttggtg 2160gacgttaacc
tttgtaatat atttttatat ttagtttaat ttttaaggga caatttttac
2220tgaaatgtga cgttccgttt tacagtattt ttaagagctt tttcctattt
gttcgactga 2280atttacacaa cattatacat tgccttttaa aagtttttct
gtaacatttt ttagattttt 2340tcaacgaagt atgtggtcca agcatgagta
aagggtgttc caaaatttac gaaagattta 2400aatttaatag gaaacgctgt
tttgtttttc tttagattgt aattttttat tgcatcataa 2460agtacgtaga
ggtggtgtga atttttgttg aatctcaccc caacagtcgc gaaaggggtt
2520taattattac cttttgccta attagacatg ggtgaatatt ttacgactac
acgcatctgt 2580cccaaagcag agagtccttc gatattaaca aactagtgaa
cttttagggc gactggttct 2640gagaatttat ttggtcg 265744155DNADiabrotica
virgifera 44tgtcatcgat aaaaagtgga ggtctaacca aaaaggaagg caagatgagg
atcagagcct 60tcccaatgac tatggatgaa aaatatgtag aaagtatatg ggctttatta
aagaatgcta 120tacaagaaat tcaaaagaaa aataactcag gttta
15545155DNADiabrotica virgifera 45cgattctgaa aaagaagatg ataaaatcaa
accatctgac aatctcatac tagtaggaca 60tgtagaaggg gatgcaagtc tattggaagt
ctacatatac aatgaacaag aagagtcatt 120gtatgttcat catgatatta
tgttatcatc ctttc 15546155DNADiabrotica virgifera 46atggaggatt
ttaaacaaag acaaggattt gcttgtaaat cgatgaagga tgtagcagat 60gctgcaggtc
cccattgggc taggatgtct aaagcagaaa gaagacctta tgaagagagg
120gcaaacaaag agaagggagg tttgagatat acttg 15547275DNADiabrotica
virgifera 47tgatgaaaag tggttccgtg cccctactag ccagactgct taaatcagac
cacatggatg 60tggtggtacc aactatcgga acgatctccc aatgtgccat cgatcccact
tatcaattgg 120ctatccagac tgaaggaatg attaaggata tagttttgca
tctattttct gaggaatatc 180cagctctcag aagatactgc tcagaaacta
tatttagatg tggagaaaat gctctgatta 240gagatatggt aagagaagct
ggtggtctgg atcct 27548275DNADiabrotica virgifera 48tgcgaaaatt
agaacattgt aatatagtga aacttaaata tttcttttac tctagtggag 60ataagaaaga
cgaggtgtac ctgaatttgg ttttggaata catccctgag acggtatata
120aagttgcacg acactacagc aaatcgaagc aaactatacc aattagcttc
atcaagttat 180acatgtacca gttgtttcgg tcactagcat acattcactc
tttagggata tgccataggg 240acataaaacc gcaaaacttg ttgttagatc cagag
27549275DNADiabrotica virgifera 49tgaaaattct caggaaagca gacatgttag
aaaaagaaca ggtggcgcat gtgagagcag 60aacgggatat tttagtagaa gccgatcatc
agtgggtagt aaaaatgtat tatagtttcc 120aagatcccat taatctatat
ttaataatgg aatttctacc gggaggtgat atgatgaccc 180tactcatgaa
aaaagacacc ctttctgaag atagtacgca gttttatata acagaaacag
240cactagccat tgattcgatt cataaactcg gtttc 27550276DNADiabrotica
virgifera 50atgacgaatt tgaagatgcc tccgaaagtt tttacagttg atgatgagta
ttttgttgac 60aatgaaggag caaatgcaaa aagaacagac ccattaattc cagaggatgt
agaagatgaa 120attgtaggct gtataacatt tgcagaacag tttacgacaa
gatacggtcc tgtccatcct 180gccttctatc agggaacatt agacgatgct
atcaaagaag catgctcaaa acctgccaaa 240gaccgaaaaa ttttggctat
atacttacac cacgac 27651275DNADiabrotica virgifera 51tcggggcatg
gaaccttttt cgattaaact agccataaaa ttttcgatcc ccttggcaga 60cttaagccta
ttacaaatat tactaattaa attataactc taacgccaca tcgcgaggct
120tcaaagtaga tacgtgcata catttcctgg tcttgtattt gtcttgtatg
gcgtgttgag 180gtaaagtcat agcggaaaca aacatctggt ataaaaacga
gatgccagat ctgatttgaa 240gaccttcgta gcctgtagat tgaacgagtt cttta
275525385DNASpodoptera frugiperda
52atgaagtatc aaagcttggt attgatagtt agtgtggcgt ggtgctcggc tcagctcacg
60gatgacatgc agatgtttga gccggagtgc atgacggagg ataagttccc ctgcatggga
120ggagggtgta tctcggtgtc tcagtactgt gacgggaacc tggactgtga
agatggcagt 180gacgagaatt tttgcatcga acacaagccg ttccaagagt
tctgtaacga gactcatcag 240tacatgtgtc aggactccac gaagtgtgtt
cctctgtcct ggctgtgcaa caacgagccg 300gactgtgatg acggcagcga
tgagttcaac tgcaccgcgc tgccggctgt caatgttaat 360tcaacatgca
aaggcttcca atgtggagac ggcaagtgta tctctttcct gtgggtctgc
420gacggcgtgt acgactgcga agataaaagc gatgagtatg ctgaggagct
ctgcaggcac 480gtgtcccgtc cccacgccat tgtcgacggc tcgtactgcc
aggagctgca cacgatggat 540gaccggaact acaaatgtct ggacgcctcc
ttctgcctgc caagcagcat gatgtgcgat 600ggactgcagg actgccgcga
tggcagcgat gagggacctt tttgcaagga ctggaacaca 660atgtgcgaca
acttcaaatg catgggtaac gacacgcgat gctccccgga gaggtacggg
720cccacctgcc tgtgcctgcc gtcccacttc atgcgccagt acgactacat
caccaagcaa 780tgccaggacg tcaacgagtg cctgatggaa cgtcccccgt
gctcccacaa atgtatcaac 840gctgacggtc actatatttg cgaatgtgat
cctgggtaca agagggatgt gtatggatac 900ctttgttatg ctactggtcc
cgaagcgatg ctgttcttta atactcggaa cgatatcaga 960tacctgaaaa
tcaagagtaa agaaatggtc acagtggcta ccgatatcat agagggtcac
1020ggcgtatcat tcgatggcac ttacatttac tgggtggaga cggcacaggg
acatcagtct 1080atcttcaaag cgcagctcgg agacgtcaag gatacaaagg
aggtgctggt aggtctcggt 1140ctggaggacc ctggcgacat agcagtggac
tacttaggcg ggaacatata cttcagtgat 1200gcggagcgag ggaccatctc
cgcctgcagg gtcgacggct ccatctgcac caccatcaag 1260acctatgcta
aaaacccacg attcgtcaca cttgatccta agaatggtaa gatgtactgg
1320gcggactggc acgagcggcc ggtgataatg tcagctcgca tggacggtag
ccaccacgac 1380acgctggtgg acaccttgga gaacttcgcc accggcctgg
cagtggacgc gcccaacggg 1440aggctgtact tcgtcgacaa aactgtcaaa
gtcgtcatga ttgcagagaa acatgtttat 1500tctttatttg aagagccgtt
ccaccacccg tactccatct ccgtgttcga gaacacggtg 1560ttctggagcg
actggacctc caacagcata cagacgactg acaaagtgca cgggaccgcg
1620cagaagagga acgttctact caaacttgat acgcctgtac tagggatgca
catgtaccac 1680ccggtgttga tgaacacgac atcgaaccct tgcagcaaca
acaactgctc gcacctctgc 1740ttcgtcacct ccaacgccac gcacgtctgc
gcctgccccg acggcatgga gatcgagaat 1800aatcagtgtc atcatgtagg
gaactaccgt gccaagtacc tggtggtggg aagtggacag 1860ctgttcacca
agatccagta caacgcgctc ggcaacccgg agtgccacgc cacgcacttc
1920gacatcggac gcgtgcaggc catggcttat gatcggtaca gagattctct
attcatatac 1980gacggccagc ggcggaccat caactacatc aacatgagtg
acttcacgct cggcgtcacg 2040cacttgttga tctacaacgg gctggaaaat
gtcgtcgaca tggattatga ttatgtgact 2100gataacctgt acgttttgga
cgcgagtcgt cgtgtggtgg aagctgtgtc tctgaggact 2160cagaagcgcg
ccatagtgca tcggttcgac attcaagaac tgcctattag cttctgtatt
2220ctttccgact acgggaggat gttggtggct gtggtggaga gtgagatgca
caataccata 2280cacattgaca gcatcgggct cgacgggaac cagcggagac
acgtcctcat gaacaatttg 2340aaaggtcccc acattagatt gcggtacgtg
gcagaaacgg aacaggtatt catatccgat 2400gagagcaacg gcatcatcga
cttcatacat cctgaaggaa ccgggcgaga gaactatcgg 2460gagctcacct
cgacggtgac cagcctcgcc atagcagaca actacgtctt ctggacagac
2520cgaaagaccc ccaggctctt ctggtctgac atacacgagg cctcaccgaa
gataaggagg 2580atggatctgg cccttttccc aaacaccacg cagctcctaa
tccaagccac aaactcgcta 2640cctgatccga aagaccccct tctgaaccat
ccttgtctga agaacccttg ttctgacgtc 2700tgtgtccaac ttccccatga
aactccacaa gatcacccga aactggcaaa cttcgagatg 2760aagtacaagt
gtttgtgccc acctggatta ctggttaacg gtaaccagtg cgccaaacct
2820gcggcgtgtg gttcggatga gatactgtgc cacaggagca atatttgcgt
gaagcaagat 2880gctaggtgtg atggaaaggt ggactgtcct aagagtgaag
acgaggaagg ttgtatagtg 2940gatccggcaa acatatgtac atcagatgag
atattttgtc gcggcttgtg cataaataag 3000gagaaagcct ccatgtgttc
tactggtgaa aaacctaata aagcgctacc ttcgaacaac 3060tgcagcagca
cagaattcca atgcacggac acgtctatct gcatctctcg gttgcaagtc
3120tgcgaccaac acgtcgactg ccccaatgga tcggatgaac atctctccga
gtgtgacacc 3180tacgcttgtc acgaaactga gttcatgtgt gcgtccggct
cgtgtatatt caagacgtgg 3240acatgtgatg gagaccgcga ctgtaacgat
ggatctgatg aaatcaactg cgtgaacatg 3300acttgcggcc ctggtttcta
ccagtgcaga gacagggagt gcgtagaact atcgaaaagg 3360tgtgatggac
gccgggactg ttcggattac tccgacgaag aagactgtga tgaggctcaa
3420gttatcgaga aagtagaaga agccccaaaa tgtgctgcgt gggagtatac
ttgtgaaaaa 3480aatactagca tttgcttgcc tgaaactgct cggtgcaaca
tgaagacaga ctgcccaggt 3540ggcacggatg agcacggttg cgacctgcgc
tgcgccccaa aaggaatgtt tgcgtgtggg 3600caacaggtta cgtgtataac
cttgaacaaa gtctgcaacg gccgtctgga ctgtgatgat 3660gggtccgacg
agacgcctga tgcttgttct cgggtgaaca ggacctccca cttattccca
3720gtgtcccgta cattcagcga ctgtacggag ggttacaagt gcaacaacgg
tcagtgcatt 3780gaatggtcac aggtgtgtga caagaagagg gactgtgtcg
acggtacaga tgaaaacgga 3840ctctgtgata ctgcatgtgc aaacagcaca
tgtaccttca tgtgtcagcc gaccccattc 3900ggacggcgct gtctctgccc
cttcggcttc caagtgtccc aggatcagtt ctcgtgcgag 3960gacatcgacg
agtgcactga agacgtctgc tcccaaggtt gtatcaacgt gccaggatca
4020ttcctgtgct ggtgccatca tggttatgcc atcaggaggt cagaccgtcg
ctcctgcaag 4080gcgatccgcg gcaacatgtc cattctatac gtgtcgggta
actccgtgcg gtctatctca 4140gcggacgggt atgggtctat agagtacacc
gatactgacg cctcggccat cactgatatg 4200gattataatg ttaggcagaa
gaagctatac gtggcttcgg aagagggcag caagttattg 4260gaagtgaatg
agacgcagaa tgtgatcgca gtcaccaacg ttgggaaacc atcgcgggtg
4320gcagtggact gggtgacagg caacgtgtac ttcgtggaca cgactccgta
cgaccagcgc 4380atacgagtct gccacgtcaa gaggaagcgc tgcgcctccc
tgctgaaact gccgtctgat 4440gccacggtca cagcattgat agtggaacct
agttctagcc gaatgttcta ctgcgtcact 4500cggaagctgg agtcagtgat
ctggacagct aacctggctg gcagacacgt gactgacctg 4560gctactgtgc
ggaactgcac cggactggcc gcagactctt tcaagaagaa gttgtatgtt
4620gcagagacgg gcccagcaca tatcattagg atggattatg aaggggagaa
tttcaacaag 4680atcctctcag atcacccacg tcttcaagcg ccacatggac
tagtaatctt cgaagactac 4740atttactact tggaagccaa ctccttccgc
ctcagccgct gccagctata tggagccaag 4800cattgtgaaa catatgtgta
tcgcgtgttt gacgccaaca catttgtcat tcgccacgag 4860agcatccaac
gtgatgacat cgtcaatgag tgtgaagatg tggtctgtga caatatttgc
4920gccgtggacg aagatgggcc caaatgtctg tgtgatgatg gagctttggc
gaagcgtggg 4980aagtgtcccg aggttgataa gaaactggta ccattattca
acggctggtc gtacgaggaa 5040ctgaaatcag ctcacagcgt ctccttcacc
atcatcgttg gagtgctgag cctaatcgct 5100atataccttg gcgtgttcgt
gtattaccac ttcgtatacc tacccaggaa gagaatgcta 5160gctgccactt
acactgaagt gcggttccag aacacaaata gttcgcccta tcccgaatca
5220gacccaactg tggagatgca tccctcaagt tccgtttcac acgagttcat
aaaccctctt 5280cagttcgtaa gaaacatgtg gtatgggccc tttaggaaag
atagacgacc aaattttagg 5340aaagatagac gaccaaatgt aagtttaaac
accacttggc attaa 5385537020DNASpodoptera frugiperda 53tctctacgtt
ctcaatttcc gttccgttgc tcttccgccg tgccagcaac cacgtgtatc 60aattaaaata
ataattaatc aatttattaa tttaataaat caattttaat aattaaacaa
120tcgttatttt ttttacttat taagtatcaa tgaaggaaat ataaataatg
aagtatcaaa 180gcttggtatt gatagttagt gtggcgtggt gctcggctca
gctcacggat gacatgcaga 240tgtttgagcc ggagtgcatg acggaggata
agttcccctg catgggagga gggtgtatct 300cggtgtctca gtactgtgac
gggaacctgg actgtgaaga tggcagtgac gagaattttt 360gcatcgaaca
caagccgttc caagagttct gtaacgagac tcatcagtac atgtgtcagg
420actccacgaa gtgtgttcct ctgtcctggc tgtgcaacaa cgagccggac
tgtgatgacg 480gcagcgatga gttcaactgc accgcgctgc cggctgtcaa
tgttaattca acatgcaaag 540gcttccaatg tggagacggc aagtgtatct
ctttcctgtg ggtctgcgac ggcgtgtacg 600actgcgaaga taaaagcgat
gagtatgctg aggagctctg caggcacgtg tcccgtcccc 660acgccattgt
cgacggctcg tactgccagg agctgcacac gatggatgac cggaactaca
720aatgtctgga cgcctccttc tgcctgccaa gcagcatgat gtgcgatgga
ctgcaggact 780gccgcgatgg cagcgatgag ggaccttttt gcaaggactg
gaacacaatg tgcgacaact 840tcaaatgcat gggtaacgac acgcgatgct
ccccggagag gtacgggccc acctgcctgt 900gcctgccgtc ccacttcatg
cgccagtacg actacatcac caagcaatgc caggacgtca 960acgagtgcct
gatggaacgt cccccgtgct cccacaaatg tatcaacgct gacggtcact
1020atatttgcga atgtgatcct gggtacaaga gggatgtgta tggatacctt
tgttatgcta 1080ctggtcccga agcgatgctg ttctttaata ctcggaacga
tatcagatac ctgaaaatca 1140agagtaaaga aatggtcaca gtggctaccg
atatcataga gggtcacggc gtatcattcg 1200atggcactta catttactgg
gtggagacgg cacagggaca tcagtctatc ttcaaagcgc 1260agctcggaga
cgtcaaggat acaaaggagg tgctggtagg tctcggtctg gaggaccctg
1320gcgacatagc agtggactac ttaggcggga acatatactt cagtgatgcg
gagcgaggga 1380ccatctccgc ctgcagggtc gacggctcca tctgcaccac
catcaagacc tatgctaaaa 1440acccacgatt cgtcacactt gatcctaaga
atggtaagat gtactgggcg gactggcacg 1500agcggccggt gataatgtca
gctcgcatgg acggtagcca ccacgacacg ctggtggaca 1560ccttggagaa
cttcgccacc ggcctggcag tggacgcgcc caacgggagg ctgtacttcg
1620tcgacaaaac tgtcaaagtc gtcatgattg cagagaaaca tgtttattct
ttatttgaag 1680agccgttcca ccacccgtac tccatctccg tgttcgagaa
cacggtgttc tggagcgact 1740ggacctccaa cagcatacag acgactgaca
aagtgcacgg gaccgcgcag aagaggaacg 1800ttctactcaa acttgatacg
cctgtactag ggatgcacat gtaccacccg gtgttgatga 1860acacgacatc
gaacccttgc agcaacaaca actgctcgca cctctgcttc gtcacctcca
1920acgccacgca cgtctgcgcc tgccccgacg gcatggagat cgagaataat
cagtgtcatc 1980atgtagggaa ctaccgtgcc aagtacctgg tggtgggaag
tggacagctg ttcaccaaga 2040tccagtacaa cgcgctcggc aacccggagt
gccacgccac gcacttcgac atcggacgcg 2100tgcaggccat ggcttatgat
cggtacagag attctctatt catatacgac ggccagcggc 2160ggaccatcaa
ctacatcaac atgagtgact tcacgctcgg cgtcacgcac ttgttgatct
2220acaacgggct ggaaaatgtc gtcgacatgg attatgatta tgtgactgat
aacctgtacg 2280ttttggacgc gagtcgtcgt gtggtggaag ctgtgtctct
gaggactcag aagcgcgcca 2340tagtgcatcg gttcgacatt caagaactgc
ctattagctt ctgtattctt tccgactacg 2400ggaggatgtt ggtggctgtg
gtggagagtg agatgcacaa taccatacac attgacagca 2460tcgggctcga
cgggaaccag cggagacacg tcctcatgaa caatttgaaa ggtccccaca
2520ttagattgcg gtacgtggca gaaacggaac aggtattcat atccgatgag
agcaacggca 2580tcatcgactt catacatcct gaaggaaccg ggcgagagaa
ctatcgggag ctcacctcga 2640cggtgaccag cctcgccata gcagacaact
acgtcttctg gacagaccga aagaccccca 2700ggctcttctg gtctgacata
cacgaggcct caccgaagat aaggaggatg gatctggccc 2760ttttcccaaa
caccacgcag ctcctaatcc aagccacaaa ctcgctacct gatccgaaag
2820acccccttct gaaccatcct tgtctgaaga acccttgttc tgacgtctgt
gtccaacttc 2880cccatgaaac tccacaagat cacccgaaac tggcaaactt
cgagatgaag tacaagtgtt 2940tgtgcccacc tggattactg gttaacggta
accagtgcgc caaacctgcg gcgtgtggtt 3000cggatgagat actgtgccac
aggagcaata tttgcgtgaa gcaagatgct aggtgtgatg 3060gaaaggtgga
ctgtcctaag agtgaagacg aggaaggttg tatagtggat ccggcaaaca
3120tatgtacatc agatgagata ttttgtcgcg gcttgtgcat aaataaggag
aaagcctcca 3180tgtgttctac tggtgaaaaa cctaataaag cgctaccttc
gaacaactgc agcagcacag 3240aattccaatg cacggacacg tctatctgca
tctctcggtt gcaagtctgc gaccaacacg 3300tcgactgccc caatggatcg
gatgaacatc tctccgagtg tgacacctac gcttgtcacg 3360aaactgagtt
catgtgtgcg tccggctcgt gtatattcaa gacgtggaca tgtgatggag
3420accgcgactg taacgatgga tctgatgaaa tcaactgcgt gaacatgact
tgcggccctg 3480gtttctacca gtgcagagac agggagtgcg tagaactatc
gaaaaggtgt gatggacgcc 3540gggactgttc ggattactcc gacgaagaag
actgtgatga ggctcaagtt atcgagaaag 3600tagaagaagc cccaaaatgt
gctgcgtggg agtatacttg tgaaaaaaat actagcattt 3660gcttgcctga
aactgctcgg tgcaacatga agacagactg cccaggtggc acggatgagc
3720acggttgcga cctgcgctgc gccccaaaag gaatgtttgc gtgtgggcaa
caggttacgt 3780gtataacctt gaacaaagtc tgcaacggcc gtctggactg
tgatgatggg tccgacgaga 3840cgcctgatgc ttgttctcgg gtgaacagga
cctcccactt attcccagtg tcccgtacat 3900tcagcgactg tacggagggt
tacaagtgca acaacggtca gtgcattgaa tggtcacagg 3960tgtgtgacaa
gaagagggac tgtgtcgacg gtacagatga aaacggactc tgtgatactg
4020catgtgcaaa cagcacatgt accttcatgt gtcagccgac cccattcgga
cggcgctgtc 4080tctgcccctt cggcttccaa gtgtcccagg atcagttctc
gtgcgaggac atcgacgagt 4140gcactgaaga cgtctgctcc caaggttgta
tcaacgtgcc aggatcattc ctgtgctggt 4200gccatcatgg ttatgccatc
aggaggtcag accgtcgctc ctgcaaggcg atccgcggca 4260acatgtccat
tctatacgtg tcgggtaact ccgtgcggtc tatctcagcg gacgggtatg
4320ggtctataga gtacaccgat actgacgcct cggccatcac tgatatggat
tataatgtta 4380ggcagaagaa gctatacgtg gcttcggaag agggcagcaa
gttattggaa gtgaatgaga 4440cgcagaatgt gatcgcagtc accaacgttg
ggaaaccatc gcgggtggca gtggactggg 4500tgacaggcaa cgtgtacttc
gtggacacga ctccgtacga ccagcgcata cgagtctgcc 4560acgtcaagag
gaagcgctgc gcctccctgc tgaaactgcc gtctgatgcc acggtcacag
4620cattgatagt ggaacctagt tctagccgaa tgttctactg cgtcactcgg
aagctggagt 4680cagtgatctg gacagctaac ctggctggca gacacgtgac
tgacctggct actgtgcgga 4740actgcaccgg actggccgca gactctttca
agaagaagtt gtatgttgca gagacgggcc 4800cagcacatat cattaggatg
gattatgaag gggagaattt caacaagatc ctctcagatc 4860acccacgtct
tcaagcgcca catggactag taatcttcga agactacatt tactacttgg
4920aagccaactc cttccgcctc agccgctgcc agctatatgg agccaagcat
tgtgaaacat 4980atgtgtatcg cgtgtttgac gccaacacat ttgtcattcg
ccacgagagc atccaacgtg 5040atgacatcgt caatgagtgt gaagatgtgg
tctgtgacaa tatttgcgcc gtggacgaag 5100atgggcccaa atgtctgtgt
gatgatggag ctttggcgaa gcgtgggaag tgtcccgagg 5160ttgataagaa
actggtacca ttattcaacg gctggtcgta cgaggaactg aaatcagctc
5220acagcgtctc cttcaccatc atcgttggag tgctgagcct aatcgctata
taccttggcg 5280tgttcgtgta ttaccacttc gtatacctac ccaggaagag
aatgctagct gccacttaca 5340ctgaagtgcg gttccagaac acaaatagtt
cgccctatcc cgaatcagac ccaactgtgg 5400agatgcatcc ctcaagttcc
gtttcacacg agttcataaa ccctcttcag ttcgtaagaa 5460acatgtggta
tgggcccttt aggaaagata gacgaccaaa ttttaggaaa gatagacgac
5520caaatgtaag tttaaacacc acttggcatt aaatgaccat aagtagacag
ggtattcaag 5580gtctattgaa aatttctagt actcattact tgtgtccaac
cgatactgga tttttgccaa 5640aaccgaaacc gaactttggg ccatggtttg
gtcgtggtgg ttatataatc ttaatctaga 5700aaaacaataa gcatcttcat
aaaatcattc atgtatcttt aatcataaca aatcaaagca 5760ttaaactgaa
gcatacattt gtcttatatt ttttactttt agccgaaact ataccgaggc
5820cgaaacttag gatccggttt cggccagaaa tccaatttca gtagacgcta
cataataagt 5880acttttgaaa ttttcttgtc acacaatcca ggcctatttg
aatttaaata atcgttattt 5940tgtataaaat aaatgtaatt tgttgcaggt
catctccggt ctaccagtca ccacgcccgc 6000atccccgccg caaccagatt
tctccgacac agaatccgac ttagacgata aagagagtca 6060gagaatttta
aaatataatt aattttattc aatgagccta caaaataatt taatttttaa
6120tctgtagcac gtttattttt attcacttga ttaatgatca agctaagaat
tattttgtaa 6180ttattatttt tattgcttta ttttttatgt tttaataatt
taattattat gattgtgtgt 6240aattgaatta tgtgttttat attatgttag
tataaaatgt acaaagttta atttattgaa 6300attaaaatta atgtaatttt
tcatagtcca atcatttggt attggtgatt atacattttg 6360ataatattat
tcaccatcaa tagcctgtgt ggcagttcac tgctgaacat tgagggttag
6420ccacgatctc ttttcgaatc tcatctttat tcattcatat cctggccagt
tcggcaagaa 6480ttcatattat ccacactacc ttaaaatgga taaacttttt
aaactatctt tttcatacaa 6540aatgactgga ctatgaatac tgaaataatt
ataattgtat tgttatttat gaattattta 6600ttcgatttgc agagttatag
ataatacaaa tcaataaatt ggtgtgccat cgagtaagta 6660attaatactt
attccaataa aagtatcgcg tggaacagtt tttgttattt tcacattata
6720aagacacacg gatgccttgc gacttactca cttatttaat ttatcacgaa
aatagaaacg 6780aacattttac actcacaata tgcaatacaa attgctattt
ttacaaaaac atttacgtaa 6840ctaaaaaaac gcgccaaatt atccataacg
gaagtaggga gtctagtgtt gtccacacat 6900atgactatca aactaaatac
gatcactaat tatttatata tttgcaacca atagattttt 6960tcaactattc
aacgttgtgt taaatgtgaa taaattaatt taatttaatt agtcaaaaaa
70205440DNAArtificial SequencePrimer 54tgttaatttt gttctttgtg
ctttcaacgt tattaaatgc 405540DNAArtificial SequencePrimer
55caggaatgct tcgcttatac atcttccatt tcgacaattg 405639DNAArtificial
SequencePrimer 56ctttcaacgt tattaaatgc agtcgtttca acgaataat
395739DNAArtificial SequencePrimer 57atcacaatcc agttcatccg
atccatctcc acaattgtc 395839DNAArtificial SequencePrimer
58tcaccaataa tatacactgg cccaatggtt tggctctag 395940DNAArtificial
SequencePrimer 59tatgatgatt tttaccatta aacttatcac aagtttgaat
406040DNAArtificial SequencePrimer 60agaggataac gtcaacatcc
acttagtgtc agtaacacgt 406140DNAArtificial SequencePrimer
61ttgccgatag ctcccatcca gctggacatg cacaaactat 406240DNAArtificial
SequencePrimer 62aatattaatt ccactactat tagtattcct tggagcagta
406340DNAArtificial SequencePrimer 63ttgccgatag ctcccatcca
gctggacatg cacaaactat 406440DNAArtificial SequencePrimer
64cctgtgtgat ggtgaaaatg attgtaataa ctttttggat 406540DNAArtificial
SequencePrimer 65cgagtgaaga tctatgcaag ttttattgtt actacacaag
406640DNAArtificial SequencePrimer 66gtctgtgata ataagttaga
ttgtcccgat tattctgatg 406740DNAArtificial SequencePrimer
67cttgatttaa atctttagcc actgtaaaat aatctaaaga 406840DNAArtificial
SequencePrimer 68tagtgggaat agcatacgat ggacatcata tttactggac
406940DNAArtificial SequencePrimer 69gaacggtttg tcctgggatc
catccataag tgaatatcca 407040DNAArtificial SequencePrimer
70cactcgtgga agaacataaa aatttaatct atggaatcag 407140DNAArtificial
SequencePrimer 71caaatgtata aattattact ttttggatca aaatccattg
407240DNAArtificial SequencePrimer 72cgatacggag aggctagtcg
tagaaataat tgatatgaat 407340DNAArtificial SequencePrimer
73ttaaaaccta tcgtaatttt cttctcataa gactcgctag 407440DNAArtificial
SequencePrimer 74atggttttac atgtaataaa agggtaacct gcgacaataa
407540DNAArtificial SequencePrimer 75acaatgctca tctgaactat
cagtacagtc atattctcca 407640DNAArtificial SequencePrimer
76tcttcagaag tttgtcagtc taactatttt aagtgtgaca 407740DNAArtificial
SequencePrimer 77tggttattat tgcatactga ggtcatattt atgcatgcac
407840DNAArtificial SequencePrimer 78cgattgtttt gatggctctg
atgaagatgg cctctgttct 407940DNAArtificial SequencePrimer
79ggaaattatt gtaacaatcg caagaatagc ctccttcttt 408040DNAArtificial
SequencePrimer 80gaaagtatac acgaagtaag ctacaaagga ggaccggtta
408140DNAArtificial SequencePrimer 81tagcttacaa ctgactatat
aaccagttgc aacagtatag 408240DNAArtificial SequencePrimer
82tatggagaca gatcctgcaa tcccagatat aaattacaca
408340DNAArtificial SequencePrimer 83tcttcggcca caattatcgg
atttgtgtac tcgtgttgtc 408440DNAArtificial SequencePrimer
84tcttgtaaag ctcacggacc aagaatgacc atatacttag 408540DNAArtificial
SequencePrimer 85ccatccaagt ttacactgta tattacgaag cttggtgttt
408640DNAArtificial SequencePrimer 86cgccatgcct gagggctaca
tccaggagcg caccatcttc 408740DNAArtificial SequencePrimer
87ccaggatgtt gccatcctcc ttgaaatcgg tgccggtcag 408840DNAArtificial
SequencePrimer 88atgttacgtc ctgtagaaac cccaacccgt gaaatcaaaa
408940DNAArtificial SequencePrimer 89ttaaaactgc ctggcacagc
aattgcccgg ctttcttgta 409020DNADiabrotica virgifera 90aactctcgaa
actgatggcc 209123DNADiabrotica virgifera 91caaacgatct tgttactgca
tcc 2392147DNADiabrotica virgifera 92aactctcgaa actgatggcc
attgcgaaga tggtcattgg aagtgtgccg ataaattgtg 60tattccactt gacatggtat
gtaatggcgc acctgaatgt ttggatggat cagatgaaac 120tattggatgc
agtaacaaga tcgtttg 1479320DNADiabrotica virgifera 93tgggaaatag
ccacatcgac 209422DNADiabrotica virgifera 94cccagtactt agatcggcaa ag
2295140DNADiabrotica virgifera 95tgggaaatag ccacatcgac cgttttttca
tggatggtac tggaagaact cacgtcatcg 60aaaacggttt agtgggccca gttcgtgtag
tatacgaccg cagtctttat agaattttct 120ttgccgatct aagtactggg
1409618DNADiabrotica virgifera 96acacgtttgc cagtggtc
189725DNADiabrotica virgifera 97tgtgtccagt acaaacattt tctac
259878DNADiabrotica virgifera 98acacgtttgc cagtggtcaa tttgttattg
agcaggaatc gcttcagcct aaagtagaaa 60atgtttgtac tggacaca
789923DNADiabrotica virgifera 99ctaactctgg catcgaatac ctc
2310020DNADiabrotica virgifera 100tgggcgtttc aaggtagatg
2010177DNADiabrotica virgifera 101ctaactctgg catcgaatac ctccgcacat
tcttacactt acctggagaa attgtcccat 60ctaccttgaa acgccca
77102474DNADiabrotica virgifera 102atgttgatgc caaaaaagaa tagagtatgt
atttacgaat acctcttcaa agagggagtc 60atggtagcta aaaaagatta ccatgcccca
aaacacctcg aactagaaac tatccctaac 120cttcaagtaa ttaaggcttt
acaatcactt aaatcaaaag gttacgtaaa ggaacaattc 180gcctggaggc
attattattg gtatttgact aactctggca tcgaatacct ccgcacattc
240ttacacttac ctggagaaat tgtcccatct accttgaaac gcccagcaag
gacagaaacc 300acccgtccta gaccagctgc tctcagatct gagacatcta
aaccttcaga agaccgtgca 360ggatacagaa ggactcctgg aggccctgga
gctgacaaga aagctgatgt tggtccagga 420actggagatg ttgagttcag
gcaaggattc ggacgtggac gggcaccaca ataa 474103570DNADiabrotica
virgifera 103ggggctttct gatttttgac agcttctata gaagtttatc aagatgttga
tgccaaaaaa 60gaatagagta tgtatttacg aatacctctt caaagaggga gtcatggtag
ctaaaaaaga 120ttaccatgcc ccaaaacacc tcgaactaga aactatccct
aaccttcaag taattaaggc 180tttacaatca cttaaatcaa aaggttacgt
aaaggaacaa ttcgcctgga ggcattatta 240ttggtatttg actaactctg
gcatcgaata cctccgcaca ttcttacact tacctggaga 300aattgtccca
tctaccttga aacgcccagc aaggacagaa accacccgtc ctagaccagc
360tgctctcaga tctgagacat ctaaaccttc agaagaccgt gcaggataca
gaaggactcc 420tggaggccct ggagctgaca agaaagctga tgttggtcca
ggaactggag atgttgagtt 480caggcaagga ttcggacgtg gacgggcacc
acaataaatt tattgataag ttaattttta 540taaattgatc agccaataaa
aagtttggtt 570104155DNAArtificial SequencePrimer 104cgccatgcct
gagggctaca tccaggagcg caccatcttc ttcgaggatg acggcaacta 60caagtcgcgc
gccgaggtga agttcgaggg cgataccctg gtgaatcgca tcgagctgac
120cggcaccgat ttcaaggagg atggcaacat cctgg 155105155DNAArtificial
SequencePrimer 105atgttacgtc ctgtagaaac cccaacccgt gaaatcaaaa
aactcgacgg cctgtgggca 60ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt
ggtgggaaag cgcgttacaa 120gaaagccggg caattgctgt gccaggcagt tttaa
1551061770PRTDiabrotica virgifera 106Met Leu Ile Leu Phe Phe Val
Leu Ser Thr Leu Leu Asn Ala Val Val 1 5 10 15 Ser Thr Asn Asn Asn
Asp Cys Pro Pro His Gln Phe Arg Cys Thr Asn 20 25 30 Ser Lys Cys
Ile Asp Phe Gln Gln Arg Cys Asp Gly Ser Asp Asn Cys 35 40 45 Gly
Asp Gly Ser Asp Glu Leu Asp Cys Asp His Lys Cys His Glu His 50 55
60 Phe Phe Asn Cys Arg Asn Gly Arg Cys Ile Ser Glu Ala Phe Leu Cys
65 70 75 80 Asp Gly Glu Asn Asp Cys Asn Asn Phe Leu Asp Glu Lys Asp
Cys Lys 85 90 95 Gly Gln Thr Val Leu Thr Leu Glu Thr Asp Gly His
Cys Glu Asp Gly 100 105 110 His Trp Lys Cys Ala Asp Lys Leu Cys Ile
Pro Leu Asp Met Val Cys 115 120 125 Asn Gly Ala Pro Glu Cys Leu Asp
Gly Ser Asp Glu Thr Ile Gly Cys 130 135 140 Ser Asn Lys Ile Val Cys
Asp Gly Phe Lys Cys Lys Asn Gly His Cys 145 150 155 160 Ile Pro Thr
Glu Trp Ala Cys Asp Gly Leu Asp Asp Cys Gly Asp Lys 165 170 175 Thr
Asp Glu Gln Asp Cys Ala Asn His Val Pro Leu Asp Gln Cys Thr 180 185
190 Leu Asp Lys Arg Lys Phe Leu Cys Ser Asn Asn Lys Thr Cys Ile Asp
195 200 205 Leu His Ser Val Cys Asp Asn Lys Leu Asp Cys Pro Asp Tyr
Ser Asp 210 215 220 Glu Gly Lys Gln Cys Asn Ser Ser Ala Ile Ser Cys
Asn Asn Asn Lys 225 230 235 240 Cys Ser His Thr Cys Ile Ser Leu Pro
Thr Gly Pro Lys Cys Leu Cys 245 250 255 Pro Asn Gly Tyr His Thr Leu
Asp Asp Ser Asn Cys Leu Asp Ile Asn 260 265 270 Glu Cys Thr Thr Tyr
Gly Ile Cys Asp Gln Lys Cys Arg Asn Leu Pro 275 280 285 Gly Ser Tyr
Glu Cys Tyr Cys Asp Asp Lys Tyr Thr Leu Gln Ala Asp 290 295 300 Lys
Lys Thr Cys Lys Ala Thr Gly Gly Ser Gly Ile Met Ile Phe Ser 305 310
315 320 Ser Lys Gln Glu Ile Arg Ala Leu Thr Leu Asp Ser Leu Asp Tyr
Phe 325 330 335 Thr Val Ala Lys Asp Leu Asn Gln Val Val Gly Ile Ala
Tyr Asp Gly 340 345 350 His His Ile Tyr Trp Thr Asp Val Phe Thr Gly
His Glu Thr Ile Ser 355 360 365 Arg Ser Ile Glu Asp Gly Ser Glu Arg
Glu Val Leu Val Thr Ser Gly 370 375 380 Leu Ser Leu Pro Glu Asp Leu
Ala Tyr Asp Trp Leu Thr Gly Asn Ile 385 390 395 400 Tyr Phe Thr Asp
Ala Leu Lys Gln His Val Gly Val Cys Ser Asn Asp 405 410 415 Gly His
His Cys Thr Val Leu Val Asn Lys Asp Ile Arg Lys Pro Arg 420 425 430
Gly Ile Val Val Asn Val Glu Ala Gly Asp Met Tyr Trp Ser Asp Trp 435
440 445 Gly Val Pro Ala Ala Ile Gly Tyr Ser Leu Met Asp Gly Ser Gln
Asp 450 455 460 Lys Pro Phe Val Thr Asn Asn Ile His Trp Pro Asn Gly
Leu Ala Leu 465 470 475 480 Asp Gln Pro Asn Ser Arg Leu Tyr Trp Thr
Asp Ala Lys Lys Met Thr 485 490 495 Leu Glu Ser Ile Asn Leu Asp Gly
Thr Asp Gln Arg Ile Val Leu Glu 500 505 510 Gly Ile Val Lys His Pro
Tyr Ala Ile Ala Val Phe Glu Asn Lys Leu 515 520 525 Tyr Trp Ser Asp
Trp Asp Ser His Thr Ile Gln Thr Cys Asp Lys Phe 530 535 540 Asn Gly
Lys Asn His His Thr Leu Val Glu Glu His Lys Asn Leu Ile 545 550 555
560 Tyr Gly Ile Ser Ile Phe His Tyr Ala Leu Glu Lys Arg Leu Val Asn
565 570 575 Pro Cys Glu His Ala Ser Cys Ser Asp Ile Cys Leu Leu Lys
Ala Gln 580 585 590 Ser Tyr Ala Cys Ala Cys Pro Glu Asn Lys Val Leu
Asp Thr Asp Gly 595 600 605 His Thr Cys Lys Glu Val Gln Pro Thr Gln
Ala Leu Val Gly Gly Thr 610 615 620 Gly His Val Leu Val Ser Ile Lys
His Gln Phe Leu Gly Lys His Asp 625 630 635 640 Val Thr Phe Leu Pro
Asp Leu Ala Lys His Val Gly Ser Leu Ala Phe 645 650 655 Asp Ser Tyr
Lys Asn Ile Leu Tyr Val Ser Asp Leu Glu Thr Lys Ser 660 665 670 Ile
Val Ala Leu Asn Met Asn Ile Gly Ile Ser Lys Thr Leu Glu Ile 675 680
685 Asp Gly Leu Gly Lys Val Thr Ser Met Asp Phe Asp Pro Lys Ser Asn
690 695 700 Asn Leu Tyr Ile Cys Asp Thr Glu Arg Leu Val Val Glu Ile
Ile Asp 705 710 715 720 Met Asn Asn Leu Glu Arg Lys Ile Ile Val His
Asp Thr Phe Gly Glu 725 730 735 Thr Pro Glu Ser Ile Ala Leu Val Pro
Glu Asp Gly Ile Met Phe Val 740 745 750 Ser Phe Lys Gln Gly Lys Leu
Gly Asn Ser His Ile Asp Arg Phe Phe 755 760 765 Met Asp Gly Thr Gly
Arg Thr His Val Ile Glu Asn Gly Leu Val Gly 770 775 780 Pro Val Arg
Val Val Tyr Asp Arg Ser Leu Tyr Arg Ile Phe Phe Ala 785 790 795 800
Asp Leu Ser Thr Gly Val Ile Glu Ser Thr Ser Ala Ala Gly Asp Asp 805
810 815 Arg His His Phe Arg Thr Leu Thr Thr His Pro Val Ser Ile Ala
Val 820 825 830 Leu Lys Asp Asp Leu Leu Trp Thr Asn Leu Asp Ser Lys
Asp Leu Tyr 835 840 845 Trp Ser Glu Lys Arg Ser Ser Glu Ser Tyr Glu
Lys Lys Ile Thr Ile 850 855 860 Gly Phe Lys Glu Asp Asn Val Asn Ile
His Leu Val Ser Val Thr Arg 865 870 875 880 Lys Gln Leu Glu Ile Asn
Ser Cys Arg Val Asn Asn Asn Gly Cys Ser 885 890 895 His Leu Cys Leu
Gln Ser His Lys Ser Ile Val Cys Ala Cys Pro Ala 900 905 910 Gly Trp
Glu Leu Ser Ala Asn Gly Phe Thr Cys Asn Lys Arg Val Thr 915 920 925
Cys Asp Asn Lys Glu Met Leu Cys Pro His Ser Asn Thr Cys Val Leu 930
935 940 Lys Ser Leu Arg Cys Asn Gly Phe Lys Asp Cys Ala Phe Gly Glu
Asp 945 950 955 960 Glu Trp Asp Cys Gln Thr Val Ser Gln Cys Leu Pro
Gly Gln Tyr Lys 965 970 975 Cys Asp Asp Gly Gln Cys Ile Ser Glu Asp
Leu Val Cys Asn His Ser 980 985 990 Tyr Asp Cys Lys Asp Lys Ser Asp
Glu Tyr Gly Cys Ala Asp Lys Asn 995 1000 1005 Lys Lys Leu Gly Cys
Pro Pro Gly His Phe Thr Cys Lys Ser Ser 1010 1015 1020 Glu Cys Ile
Ser Glu Arg Phe Val Cys Asp Ala Phe His Asp Cys 1025 1030 1035 Asp
Asp Gly Ser Asp Glu Leu Asn Cys Glu Asn Asn Ile Cys Leu 1040 1045
1050 Glu Ser Gln Phe Arg Cys Asp Val Gly Thr Cys Ile Pro Lys Asp
1055 1060 1065 Trp Glu Cys Asp Gly Glu Tyr Asp Cys Thr Asp Ser Ser
Asp Glu 1070 1075 1080 His Cys Ser Ser Glu Val Cys Gln Ser Asn Tyr
Phe Lys Cys Asp 1085 1090 1095 Asn Asn Arg Cys Ile Asp Pro Lys Leu
Gln Cys Asp Gly Phe Asp 1100 1105 1110 Asp Cys Gly Asp His Ser Asp
Glu Lys Phe Glu Lys Cys Leu His 1115 1120 1125 His Ser Lys Ala Pro
Lys Cys Thr Leu Glu Glu Phe Ala Cys Ile 1130 1135 1140 Thr Asn Thr
Ser Ile Cys Leu Pro Lys Ser Ala Lys Cys Asn Gly 1145 1150 1155 Thr
Ser Glu Cys Pro Asn Asn Glu Asp Glu Lys Asp Cys Ser Lys 1160 1165
1170 Cys Glu Glu Asp Glu Phe Glu Cys Lys His Lys His Tyr Arg Glu
1175 1180 1185 Cys Ile Pro Arg Ser Trp Ile Cys Asp Gly Thr Asp Asp
Cys Gly 1190 1195 1200 Asp Asn Ser Asp Glu Ser Met Glu Thr Cys Ser
Ser Lys Leu Lys 1205 1210 1215 Pro Ala Ala Asp Asn Phe Ala Val Thr
Asp Ser Cys Leu Thr Gly 1220 1225 1230 Tyr Arg Cys His Ser Gly Ala
Cys Ile Asn Met Thr Ser Val Cys 1235 1240 1245 Asn Asn Asn His Asp
Cys Phe Asp Gly Ser Asp Glu Asp Gly Leu 1250 1255 1260 Cys Ser Ser
Ser Cys Val Gly Val Lys Asn Pro Cys Asn Gln Ile 1265 1270 1275 Cys
Val Lys Thr Pro Ser Gly Pro Arg Cys Glu Cys Lys Pro Gly 1280 1285
1290 Tyr Lys Leu Leu Gly Asp Gly Lys Thr Cys Ile Asp Asp Asn Glu
1295 1300 1305 Cys Gln Thr Asp Pro Pro Ile Cys Ser Gln Leu Cys His
Asn Lys 1310 1315 1320 Glu Gly Gly Tyr Ser Cys Asp Cys Tyr Asn Asn
Phe Leu Leu Ser 1325 1330 1335 Ser Asn Lys Lys Ser Cys Lys Ala His
Gly Pro Arg Met Thr Ile 1340 1345 1350 Tyr Leu Val Ile Tyr Gly Asn
Gln Ile Arg His Leu Ile Pro Lys 1355 1360 1365 Ser Asn Thr Met Ala
Val Leu Tyr Thr Asn Pro Met Ile Lys Ile 1370 1375 1380 Ser Ser Leu
Asp Thr Leu Val Lys Pro Lys Leu Ile Phe Phe Ser 1385 1390 1395 Ser
Tyr Glu Thr Gln Ala Ile Tyr Lys Leu Asp Thr Thr Thr Asn 1400 1405
1410 Met Met His Tyr Ile Arg Asn Val Gly Phe Pro Arg Thr Ile Ala
1415 1420 1425 Val Asp Trp Ser Thr Gln Asn Ile Tyr Tyr Phe Asp Thr
Asp Val 1430 1435 1440 Asn Gly Arg Ser Ile Ser Val Cys Ser Phe Glu
Glu Lys Cys Ala 1445 1450 1455 Lys Leu Ile Asn Ile Glu Ser Pro Arg
His Val Thr Ala Leu Ala 1460 1465 1470 Val Asp Ser Val Asn Lys Leu
Leu Phe Tyr Val Leu Arg Asn Trp 1475 1480 1485 Trp Val Leu Glu Thr
Pro Ser Phe Val Ile Tyr Ser Val Asn Leu 1490 1495 1500 Asp Gly Ser
Asn Arg Gln Glu Ile Val Lys Thr Thr Thr Gly Asn 1505 1510 1515 Val
Glu Asp Ile Thr Phe Asp Ile Asn Lys Lys Leu Leu Tyr Tyr 1520 1525
1530 Thr Asn Lys Gln Asp Glu Ser Ile His Glu Val Ser Tyr Lys Gly
1535 1540 1545 Gly Pro Val Lys Thr Val Phe Ser Asn Ile Ser Gln Pro
Glu Gly 1550 1555 1560 Leu Lys Phe Phe Glu Asn Gln Leu Tyr Tyr Thr
Val Ala Thr Gly 1565 1570 1575 Tyr Ile Val Ser Cys Lys Leu Tyr Gly
Asp Arg Ser Cys Asn Pro 1580 1585 1590 Arg Tyr Lys Leu His Thr Phe
Ala Ser Gly Gln Phe Val Ile Glu 1595 1600 1605 Gln Glu Ser Leu Gln
Pro Lys Val Glu Asn Val Cys Thr Gly His 1610 1615 1620 Lys Cys Pro
Tyr Leu Cys Val Pro Ala Glu Ser Gly Tyr Arg Cys 1625 1630 1635 Leu
Cys His Asn Gly Lys Val Ser Asn His Ser Glu Ile Cys Gly 1640 1645
1650 Glu Asn Asp Asp Asn Gln Ser Gly Asn Asn His Lys Phe Ala Val
1655 1660 1665 His Thr Thr Pro Ile Gln Thr Ala Ser Asp Lys His Ser
Gly Ala 1670 1675 1680 Val Ala Ser Ala Ile Leu Ile Pro Leu Leu Leu
Val Phe Leu Gly 1685 1690 1695 Ala Val Phe Tyr Tyr Leu Leu Lys Arg
Arg Gly Asp Thr Gly Leu 1700 1705
1710 Asn Ile Ser Met Arg Phe Tyr Asn Pro Leu Tyr Gly Lys Pro Val
1715 1720 1725 Arg Glu Asp Gln Lys Gln Ile Leu Lys Pro Gly Gln His
Glu Tyr 1730 1735 1740 Thr Asn Pro Ile Ile Val Ala Glu Glu His Glu
Asp His Ala Lys 1745 1750 1755 Asp Ala Ser Arg Met Leu Asn Asp Ser
Cys Val Cys 1760 1765 1770 1075498DNADiabrotica virgifera
107taccacagtc gacatctgac agtttctaca gtatagttgc agtgttcagt
ggaaaatatt 60caattaagga acctatatat tgttgactct gctacgcccg tcatgttggc
acaactctcg 120attatgttcc gaacagtgtt tgtgggattc tgaaaaacca
gagcatcgtg aacgttcagg 180acaatagtat tctctcttgg tgaatagaca
gacttactga ctgtacgcaa tagcaccggt 240gtaaaacttg atgacgttga
tgatgatgaa gccatcgcaa gcaataacaa aaacaagcac 300gaacgaaagg
ttaattacaa tagcactgat agtatcgttt cttggtgatt catgtatgat
360tgtgactaat cggtttgata gaaagaaaga tttattgtaa ctcctttcct
catcgacctt 420aaaaagtata tactacaatc aaaatcgttt tcaaagaact
caaagggact ggcgagcttt 480ggccgttgta gccgtgttct gttttatggc
gttctatcgt ttataatgaa cagtttgcta 540taaacgacat tacactagtt
acaaatcagt tcgttgtaag tttatgatgc aaaatggaaa 600gatgtatatt
gcaggactat gtgctaatag aactgaagta cttcggaaaa taagcaagaa
660ttagctcaga gtcataacag gtttccttac aagacaagtc aagttcccct
atgggaaaag 720aagtttgaaa ataacgaaac ctggtggtgg tcaaagaagt
tcaataaaaa gaaaaagaga 780agagaaaact atataaacag tggcaagaaa
atagatcgca catagagagg aagcgaaagc 840agcaaaagct aaagcagatg
tgtgtacaac tctatacgat caacttgaca tcagttagaa 900gtaagaagtt
tacgtcagag agattgtaat ttttctatga agaaattgtt tttcgtatca
960ttagtttagt ttgttcagtg aaagagaaaa tgaaaaaaca aggtggaatt
ttaaggttat 1020accgcacacc tggttgtaca gcagcaaaaa agtttgaaat
tgtccagaaa cttcagcaaa 1080catgtaaaaa tctaaaggat attcaaaccg
aaatatgttt tcatatcgaa tcaacaagtg 1140ccttggaagc aaaagacgtt
aagattttga aatgggttct tcaagatcct ctccatcccc 1200aaaatttaag
cgaaaaggga acgctggtag cgaaaaatgg tacggctttg gttgaggtag
1260gacctagatt taatttttcc acatcaagtt ccacaaatgc tgtttctatt
tgccaaaatc 1320tcggcttggt ccaagtaacc agattagagg tctcacgtcg
ctatctttta gtgtttaatc 1380aaggtacaaa acttacacct caaattgaga
ataatctagc atctttactc tatgatagaa 1440tgactgaatg caggtatact
ccagaaaata tacctaaagt tagctttaat gaaaaacttg 1500tcaaaaagga
ggatataaaa gagattgata tattgggtaa aggtgcagaa gctttaaaag
1560ccattgataa agaacttggt ctggcgttcg atgaagcaga tatagtatat
tacacaaatt 1620tgtttaaaaa tgttcttaag aggaatccca ctaatgttga
atgctttgac ttggctcaat 1680ctaacagtga acacagtaga cattggtttt
ttaagggaaa aatggttatt gatggtgtag 1740agcataagca atcactgata
gatatgatta ttgaaactca agaacacaca aatcccaaca 1800atgttataaa
attcagtgat aatagtagtg caattaaggg attcttacac cgaagcttaa
1860ggcctgtaac agcaggttct ctcagtgagc ttaagcaagt accagtagaa
tctcatctta 1920ttttcactgc agaaactcac aatttcccga ctggtgtagc
tcctttcagt ggagccacta 1980caggaactgg aggaagaatt agggatgtgc
aaagtgtggg aaggggtggt tattgcatag 2040ctggtactgc tggttattct
gtaggcaatc ttcagattcc agattatgat ctaccatggg 2100aagacaaaaa
cttctcttat ccatcaaact ttgctccacc tttagaagtt ctagttgaag
2160ccagtaacgg agcctcggat tatggtaaca agttcggtga acccttgatt
tccggcttcg 2220tacggtcctt tggtcttgtt gacgcggctg gagagagaaa
agaatggatt aaacctatca 2280tgtttagtgg aggtattggc agcatggaag
ccgatatgac tgagaagctg ccacctaaat 2340taggacacca aatcatcaaa
attggaggac ctgtatacag aattggtgta ggtggaggat 2400ctgctagttc
agtcgaggta caaggggaca acaaagtaga gttagacttt aatgctgtac
2460aaagaggtga tgccgaaatg gaaaacaaat tgaacagagt cgtacgtgct
tgcttggaac 2520taggttccaa aaaccctatc gtcagtattc atgaccaagg
agctggaggc aatggaaatg 2580ttttgaaaga attagtagaa ccagtaggag
gaattatata cgccaataaa tttgatttag 2640gtgatccgac aataaacgtt
cttgagttgt ggggagccga atatcaagaa aataatgcgt 2700tgttgtgtaa
aaaagaagat cttcctcgtc ttaagagtat ttgtgcaaga gaaagatgcc
2760ctgttaacgt tgtaggtgaa gtaactgata caggaagagt agttctggct
ttagacgaaa 2820gcaaggctgt tacaccgttt aatttggaat tggaacatgt
attaggaaaa atgccacaga 2880aagtcttcaa gttaaacagg cacaaagtag
ccagtaaacc actagaattc ccaaaagccc 2940tttcaatcta ccaatctctg
ctgagagtat taaggttgcc atctattgga agcaagcggt 3000acttaacaaa
caaagtagat cggtgtgtta caggtttgat agctcagcag caatgtgtgg
3060gaccactaca tacaccttta gctgatgttg ctgttacggc tctttctcat
tttggacatg 3120aaggtatagc gtcatctatt ggagagcaac ctatcaaagg
tttagtgaac actgcagccg 3180gtgctagaat gacggtagtc gaagctttaa
gcaacctcgt attcgccggt atcaccagtt 3240tacgcgacgt caaatgcagt
ggtaactgga tgtgggctgc caagctccct ggcgaaggag 3300cagctctgta
tgacgcatgc aaagctatgt gtgacctcat gagcgagtta ggaatcgcca
3360tcgatggtgg aaaggattct ttgagtatgg cagctagagt tggaaaagac
actgtcaaag 3420ctccaggaac cttagtagtt tctacttatg ctccttgtcc
tgatattaga aaagttgtga 3480cccctgattt caaagcgcct tctatgggaa
aaacaggaac cattctgttc gtagatcttt 3540cccgaagtga cagtagactt
ggtggttcgg cattagctca agcatttggt caacttggag 3600aggaatctcc
cgatcttcat agcgctgaag aattagtaaa tgcatttaat gcaacacaac
3660agttaataaa agatgattca attttggcag gacacgatat cagcgatggt
ggcttaatcg 3720tatgcctatt agaaatgtgc ttcgcaggaa tatgtggtat
ggaagttgat gttcaacata 3780gacaaggcaa accacttaat attttgtttg
ctgaagaagt tggatgggta ctggaagttt 3840tggatgaaga tgttgctcac
tgcctgaatg tgttttcgaa tcacaacgtc ccagtattca 3900aaataggaaa
gactgttgga tatggagtta aatctaaatt tggaataata gccaaccatg
3960cttgcctaga aagtacaatt ctacctttga tgaagctttg ggaagagact
agttataggt 4020tagaactaca tcagactatt aaatcctgtg ccgattcgga
attccatagt cttccaagcc 4080gaactggacc caaatacaca cttacattcg
accctgatca cgatcagact gtaaaagacg 4140aattagttcc cgtagctgtg
ataagagaag aaggaacaaa cggagatagg gaaatggctg 4200ctgcgttgat
aagagccggg ttcaaagtgt gggatattac tatgcaagat ctgttgagcg
4260gtgtggccac tttggatagg tttaggggcg tgatattccc tggaggtttc
agttatgcag 4320atgtcttggg ttctgctaaa ggctgggcgg ccagtattct
attcaacaaa accgtccgag 4380aacagttcga caaattcttt gccagaagag
acacgttcag tttgggagta tgtaacggat 4440gccaattgat ggctcttatc
ggttgggttg gaacacctgt taatgaaggt actaaaccag 4500acataatgtt
ggaacataat ttgtctgaaa gattcgaatg ccggtggagc actatcaaga
4560tagaaaaatc caaagctata atgatgaagg gtatggaagg gtcttcattt
ggtgtgtggg 4620tcgctcatgg agaaggtcga ttcacgttca aatcagacac
catctacgac caattgaaat 4680ccgaagaact cgtcgccttg cgttacgtag
acgataacgg aaaaccgact gaaatctatc 4740ccatgaatcc caatggaagt
attgagggat tggcgggagt gtgctccgaa gacggaagac 4800acttagcgat
gatgccgcat cccgaaagat gcgttcaacc attccaatgg ccgtacatgc
4860cacaaagctg ggtacactac aagaagagtc catgggagaa gatgtttaga
aacgcttatg 4920aatggtgcag aagcaataaa ttccataatg tttattatta
agttaaaaga aatattgtaa 4980atatttcaat gatctagctc aaattttata
atgtttatac acattttgaa gttattctat 5040tttagatata gcagtttcga
atgtagagtt gagtccatgg atatttaccc gtgcgtcatc 5100atttacagca
tacgaaataa gtcggaaatt tacttaacgc aacagcaagt gacagaaagt
5160ggctactgct ccgatgacgg atattataaa atttgacatt atcaaataaa
tagaatgtaa 5220aatgttagtt ttgctttaaa attttggtgc agaattaaca
ctacatttga agtagcttaa 5280taaattattt taatatcatt actgattaat
aaatacttaa ataacaattt ttaaaaaatt 5340taatgaaata tatatttttg
ttatttcttt cacttttgcg gaaacttaaa taaaaccatt 5400ccttaactgt
gtgaatgagg ttaactttgt atgtaaatta atggcaaaat aaaatacact
5460taccataaaa tttcaaactc caatataaaa ttagttgt
54981081875DNADiabrotica virgifera 108ctccgtttag cgagcggaat
aacggcgtcg aatcaaacgg aaagacaaga gattgacctc 60tctaaataat ccatctgctt
gctatcagcc agcgagcaag ctacacacgg cccaagacga 120aagaacgaag
aaccttcgcc ttctctcctt cgccagttca gtatcccttc agcgacgctg
180tgcatcgttg ctggtttaca acagtcgttg tagttcttcc caccttttca
atcgctaact 240caacggttga aaaccgacga tatatcctgc ctctgccttt
gcctcatcga gtggggagaa 300atacgggcag acaacgatct gtgtaatggc
aatggcgttg tacagactat cagaactgaa 360atcatggcct agttcccttt
aaaagttcag aaaacagtga attcgcgtgg atagtgaaac 420tggtgtaaat
gagtgataaa aatttaataa ttctacgatg gctgaagcag aaaacatcga
480tttccaagca tgttgcagac tgtgtctcag cgaaatggtg gattctttaa
agtccatttt 540cgacgaaact cctgaagatg aaagtctctc ccaaaagatt
ttacaatcag tggctattga 600ggtcagttcg gcagacaaat tatccaccaa
gatctgcaaa gagtgcgtag aaaaagtaac 660tgactggtct tcgtacaaag
aacgatgtct ccaaaaccaa aagaaattaa cggagttgtt 720agttgcgaaa
gaaggtccag aagttttggt tgtacctgaa attatacccg aacccgaagc
780accacttcct gtaaatggaa ttgaatcaga aactccttct gatgacgaca
ttcatgaaaa 840ccaagtggat cctgcctctt tgttggacct tagcttgcaa
attaaagaag aaccagcaga 900ccacactgaa cagaacgaac acgataaccc
atatctagca tatttgggtt atcgtgttcg 960ttctgttcag tgtggtctgc
tggttcttct ttaatttgca agctaaggtc caacaaagag 1020gcaggatcca
cttggttttc atgaatgtcg tcatcagaag gagtttctga ttcaattcca
1080tttacaggaa gtggtgcttc gggttcgggt ataatttcag gtacaaccaa
aacttctgga 1140ccttctttcg caactaacaa ctccgttaat ttcttttggt
tttggagaca tcgttctttg 1200tacgaagacc agtcagttac tttttctacg
cactctttgc agatcttggt ggataatttg 1260tctgccgaac tgacctcaat
agccactgat tgtaaaatct tttgggagag actttcatct 1320tcaggagttt
cgtcgaaaat ggactttaaa gaatccacca tttcgctgag acacagtctg
1380caacatgctt ggaaatcgat gttttctgct tcagccatcg tagaattatt
aaatttttat 1440cactcattta caccagtttc actatccacg cgaattcact
gttttctgaa cttttaaagg 1500gaactaggcc atgatttcag ttctgatagt
ctgtacaacg ccattgccat tacacagatc 1560gttgtctgcc cgtatttctc
cccactcgat gaggcaaagg cagaggcagg atatatcgtc 1620ggttttcaac
cgttgagtta gcgattgaaa aggtgggaag aactacaacg actgttgtaa
1680accagcaacg atgcacagcg tcgctgaagg gatactgaac tggcgaagga
gagaaggcga 1740aggttcttcg ttctttcgtc ttgggccgtg tgtagcttgc
tcgctggctg atagcaagca 1800gatggattat ttagagaggt caatctcttg
tctttccgtt tgattcgacg ccgttattcc 1860gctcgctaaa cggag
18751093249DNADiabrotica virgifera 109tgattttgat gaaaaaaaat
ctgatgaaat agtagccatg aactctgacc tgggagaaaa 60aatattattc gaaaaaactg
taacatgcca gggaggtgtc gaaatttggc tgaaccatct 120tcttaatgcc
gtcagagaca ccgtcaaaaa cgtctgtgct atgcaggctc agtgttttaa
180tgatcctgaa tacgacttca tatctggatt cgttcatttt tgtggacagg
ctggactagt 240tggtatacaa atattgtgga caaaagaatc cgaaattgct
attaagaaag caagagtaga 300tagaatggct atgaaaatta caaataaacg
atttctcgac ctcttaaatg ctctcattga 360tcttacatca aaagatttaa
caaaaatgca aagaataaga tttgaaacca tggttactat 420ccacgttcat
caacgagaca ttttcgacaa catttacaag ttaaaagtta agtcattatt
480ggattttgaa tgggcttgcc agacaaggtt ttactatgat gaagagacgg
atgatataac 540tgtgaaaatt acagacgtca tctttttata ccaaaacgaa
tatttaggaa taacagagag 600gctggcgatc acccctttaa ctgacagatg
ttacataact ttggctcagg ctatttggat 660taatatggga ggagcacccg
ctggccctgc tggaacagga aagacagaaa cagtcaaaga 720tatgggaaga
actctgggaa aatttgtagt tgtattcaac tgctctgacc aaatggattt
780tagaggactt ggtcgaattt tcaagggact tgctcaatca ggaacctggg
gatgcttcga 840cgaattcaac agaattgagc tacctgtgtt atcagtagct
gctcaacaga tctacatagt 900tttagaagca agaaaagcac gcaagccatc
gtttatcttc atggatggag acaacgttag 960tatgaatata gagtttggta
tattcctcac gatgaatcct gggtacgcag gaagacaaga 1020attgccagag
aatctgaaaa ttatgttccg aagtgttgct atgatggtcc ctgatagaca
1080gatcatcatc agagttaaac tagcttcctg cggctttaaa gaaaatgttc
acttgtccaa 1140gaagttcttt accttgtatc agttatgtga agaacaactt
tctaaacagg tgcattatga 1200ttttggcctt cgaaatattt tatcagtgtt
gagaacactt ggagcacaaa agcgagcaaa 1260tccaaatgaa actgaagaag
tcgtagtaat gagagtttta agagatatga atttgagcaa 1320actaattgac
gaagatgaac ctcttttcct ttccctaata gaagatatgt tccctggtat
1380taaactaact acaaaagctt ggaaagacct gcaaaaagct atcacgcata
tatgccaaga 1440gcttggtttc gtcaattatc cttcgtggaa tcttaaggtt
gtgcaattgt atgaaacctc 1500tttggtacgg cacggactaa tggttatggg
tcccacgggt gcgggaaaaa caaaatgcat 1560gttaactcta atgaagtcta
tgacagaaat gggtatgcct catagagaac ttcgaatgaa 1620tccgaaagca
atcacggctc cccaaatgtt tggaagattg gatgttgcca cgaacgactg
1680gacggatgga atcttctcaa ctttgtggag aagaactctt aaatttaaga
aaacagacta 1740ttgttggtta attttggatg gtcctgtaga tgcagtatgg
atagaaaacc taaactcagt 1800tttagacgat aacaaaactt taactctagc
caatggcgac agaatagtga tggcaaataa 1860ctgtaaattg gtattcgaac
cggacaatgt tgataacgca agcccagcca ctgtatcccg 1920aatgggtatg
gtattcatga gttcttcggt ccttccttgg tcaccgatct tagaggcttg
1980gttgaaaact agatcagcgc aagaagctga ttctatcaga aatcatttta
acagatgcta 2040tgatgacctt cacgttttcg tgcagaccag attaaaagcc
aagatgtttg taagggaagc 2100tctgtacgta agacagtgtt acgatgtttt
acaaggggta ttagatatct ttgaagaacc 2160aaaaacatgg agtgatagac
aaatagaacg attggtacta ttctcaataa tgtggtcctt 2220gggagcactg
ttagagttag acgacagaca aaaacttgaa gaatttgctt taacccaccc
2280gtccaaaatg gattggccaa aagctcagga aggagaaacc atttttgaat
ttgtagttaa 2340tccagacaat ggaagatggg aacactggaa atccagagtt
gaaccctttg tatatcctac 2400agataccgtg ctagaattta cttcgatatt
ggtacctaat gttgacaacg ttagaacagc 2460ctttttaata catacgatag
ccaaacaggg taaagctgta ttgctaattg gtgaacccgg 2520aactgccaaa
accgttatga ttaagggtta ttgctcaact tttgatcctg aatataaact
2580gaacaaatgc tttaattttt cttctgctac tactccaaac atggttcagc
gtatcataga 2640atcttatgtc gataagagag ttggtacaac ctacggacct
cctgccggaa aaactttaac 2700catatttata gacgacatta atatgcctgt
aattaatgat tggggtgatc aaatcacaaa 2760cgaaatcgta agacagtgca
tggaaactgg aggattctat agtttggata aaccaggcga 2820tttttcagta
attgctgacg ttatgtttct gtctgctatg attcatcctg gaggtggtag
2880aaatgatatt ccccaccgtc tcaaaaggca gtttagtata ttcaactgta
cactgccaag 2940tacttctgct atggatgtta tattcagtca aattgcttgc
ggctactttt gtctagaaag 3000gtttaacgaa gaaatggcag agtttattcc
aaagctagta ccacttacgc gacatgtatg 3060gcagaatacg aagaaaaaaa
tgttacccac tccagccaaa ttccattatg tatttaatct 3120acgagatctt
tctagaatat ggcaaggaat attgacagtc caagtgcttg agtgtccgac
3180taagaatatg gctcttaagc tctggagaca cgagtgtact agagtaattt
cagataggtt 3240tactgaagc 32491101370DNADiabrotica virgifera
110cttaaacata accttcaatt tgtaatgtgg ttttaaaatt cattccttaa
ctttaaattt 60atttattttc aattaggaaa attaggctat aacgtcgaaa aatgtccaaa
aaacgctata 120attggaaagc taggcaaaat gttgaaactc tggttgataa
tactcataaa gaaaaggaaa 180tggctcctcc accatacgca gacctcggca
aaagagcaag agacgtattc ggcaacggtt 240atcactttgg cctaattaaa
ttaaattgca aaaccaaaac agccagtggg gtccaattta 300gtacaggtgg
tagttcagac catgaatctg gaaaagtgtc tggatctttg gagagcaaat
360acaccgtcaa agattatgga ttgactttca cagaaaaatg gaatacaaat
aatactttgg 420ggactgagat cgccatttca gaccaaggta ttaagggact
caaggtctct ggtaatgtta 480acttctcccc acaagaagga taccagtcag
gagttgtgaa gtctgaatac ggcaacgaaa 540gaatacacct caacgccgat
gttgacttaa atagtaacgg acctgtcgta agaggttcgg 600ctgtcgtggg
ctatcaagga tggttggctg gttaccaggc tgcctttgac gttcaaaact
660caaaattggc caagagcaat tttgcattag gattttcaac atcagacttt
gttctccata 720catgtgttaa cgacggccaa acgtttggcg gttccatcta
ccaaaaaatc aaccccaaat 780tggaaacagc catcaacttg tcttgggcag
ctagcggaga aaagaccgac ttcggtttgg 840gctgcaagta tgacttggac
gccgacgcat ctcttcgcgc taagattaac aacgccagtc 900aaatcggttt
aggataccaa caaaaactac gtgatggtgt aactctaact ttgtctaccc
960tcatcgacgg caagaacttc aatcaaggtg gtcacaagat tggtttggcc
ttggacctgg 1020aggcttaaat atgttattac atcttgttcc acaaatacat
attagcgccc atttaatata 1080tttaagaatc ctgtattgta gataataatt
atttaaacgg gactttagtg ttatacttta 1140ttactcccgt tgtgtttatt
tcagttgctc ctcccaatta ctcctttttt acacgctata 1200cgtatatatt
gtttttatta ttaaactctg aaaggcgcgc ttttctggat attttcaaag
1260attgatgtca agttaggagc atatgcattt tatgtcaaaa aattagattg
ggattgtggg 1320tcttagatgg ctacaatccg ctttgtgtag ttttgcaatt
ttgcaaaaaa 13701111194DNADiabrotica virgifera 111acccattggg
gaacgcacgc attttgttta gttccggcgt ctttgctcaa gttttgcccg 60gttgttgctg
tgtttggttt gttggtgcat tttattatta taacaagttc attacaatgg
120gcaaaaagaa ggtagaagag agtgaaagtg aagaggagta ttctgtagag
aaaattattg 180ataggagagt tgtagatgga aaagtagaat attatctaaa
atggaaagga tattcagaag 240atgataacac ttgggaacct gaggacaatc
tagattgtcc tgaacttatt tctgagtttg 300agaggaatcg taaaagtaag
aaggctgctg gcaaagaaaa gaaaagaaca agagataatt 360ctacatcttc
cgtggattca aacaattcat ccactaaaga aaaagaaaaa aagaaaaaga
420aaggccgttc tccatcaccc gagtctgaaa gtgaacccag ctctaaaaaa
tcaaaaatag 480atgattcaga tgatgacaaa aagagcaaaa agaagtcgtc
atcaaaagat gctgattccg 540atgatgataa aggaaaaaag tcaaagaaga
aagacaaaag tgctttagat gatgactctg 600acgatgagaa gtcaaaaaag
tcaaaaaaaa agattctgat tcagaagatg aaaaaaagaa 660aaagaagaga
aaaagtggtc ctaaatctcg taaagctgcc gtttcagatg actcagatga
720agatgaagcc ccaaaaaaga aaactgaaag tcgggcgaaa aaagatgatg
aaaaatccaa 780gaaaaaagct gatgattctg atgatgaaga tgatccgaag
aagagcaaaa aagttgacaa 840caaggaaaaa aagggtgcta aacctaaaga
caaagagaag tctgcatttg acaaaggttt 900agaagctgat aaaattattg
gagcatctga cactagtggt caattgatgt tcttgatgaa 960atggaagggt
acagatgata ctgatttagt ttatgcaaga caagccaatt tgaaatgccc
1020acaggtggtc attaaatttt atgaagaaag aattgcgtgg catacgccag
acgactttta 1080attttactta gtgataagat ttgttgtaga tttaatttct
tacttttaaa taggtagagt 1140tcttatatta ttaatccata tgtatttttt
ttaaatggaa gaaaccacga cttg 11941121802DNADiabrotica virgifera
112gggggagaaa acatatgatt gcccagactg acagtaattc gatattttga
attaattgaa 60ctgaacactt ctattattaa aactaatcaa aacactcgaa acagatacgt
aatgcagtta 120taatgagttt tataattatt gcttttaaaa atttcctaca
taccatgttg cagttattat 180cttttggtaa aaaaactatt acaaatagtt
tgagtgtgta tgtaaaaact attggtggca 240acactttaac tgtagatcta
gatcctacat gggacattaa aaatgttaag gaaatagtgg 300caccacaact
tggattagaa ccagatgaag taaagatcat ttttgctgga aaggaattgg
360gagacgaaat taaaattgag gaatgtgatt taggtcagaa aagtattctc
catgctgtaa 420aaattagagg aaagaggagt aagcttattc aacctacaaa
tagtagtgta atagaggaaa 480atgaagatcc aagtagcaag ccattgagtg
aggttttaga tgatagtatg ttctctcatg 540aagacagcaa ttcttttaat
agtacaactg aaaggcggtc atctcaatca gaaagtggtt 600taattaagtc
aaaagtacat ttctatgtat tctgtccaac atgtaaggca atgaagtcag
660gaaaaataag agtaaggtgc catttttgta aaagtggagc gatcactgta
catgctgatc 720cccaaaactg gaatgatgtt ctaatatcaa aacaaattac
aggaacatgt gaaaatagtc 780cagaattatg ttctaaccta ttaaaaaacg
tagagcctac ttttgctgag ttttacttca 840aatgttctga acatacatca
ttaggggaac aagatggagc agtgccacta gatctaataa 900ggccaaattt
aagggacatt ccctgtttag cctgtgcgga tgttagcgat cctgttcttg
960tctttccttg tcaagaaaaa catgttacat gcctggattg ctttcgccaa
tattgtgtga 1020ccagattaat ggaaagacag ttctggcaac atccagagct
gggatacacg ttggcttgtc 1080cagctggttg cctagactcg tttataaaag
aagtccatca ttttagattg ctcacagatg
1140ctcaatatgc gcaatatcaa cgtttcggaa ccgaagaatt cgtcctcaga
tccggcggag 1200tactgtgccc tcaaccgggt tgtggaatgg ggattttagt
tgattccagt tgtaacaagg 1260tggcgtgtat aaacggttgt ggttacgtat
tttgtcggtt atgtttacaa ggctatcata 1320tcggcgagtg tcgaccatcc
gatatggaca gttctgcagg tggtgaagga tgtttgtaca 1380gcgtagaccc
aaatcgagca tcggacgcaa ggtgggacga agcttcgaga gttaccatta
1440aagtttccac gaaaccttgt cctaaatgta ggacacctac tgaaagagat
ggcggctgta 1500tgcacatggt atgtactaga gcagggtgtg agttccattg
gtgttgggtg tgccaaactg 1560catggaccag agattgtatg ggaagccatt
ggtttggtta agacattgaa aaatatgtga 1620tattaataaa ctaaaaaatg
tgtgttcttt tgattgtaca gttgtgtttc acttaacaaa 1680ggttgtttgg
ttattttttg ctatgtttga atcaacagac cttaaaatgc cgttctgtgt
1740catacaaaag agaccacaga gtacagaaga agaatcatca gatctattat
agatatagaa 1800ta 180211315730DNADiabrotica virgifera 113agcaaatggc
atggcagatt acaatggcag cgagcagtgg cggagtagat tggacgactg 60tcttaaaacc
attcttatct aaaaattatg agacttctaa tacagcagaa ctgatagaac
120tatgttcagc tattgtaaaa agcgaatccg aaatcttgcg acacaaagag
agtaataaga 180atttcttcaa ttcctttgcg gttctagctt cagattatat
tagcagtagt actagtggtt 240tacttgcgag tcaactagaa acggtaaacg
ctgcatgtcg tatactatta aagtatctgc 300ttagtgctct cagtagcgca
tgtagcgaaa ccaatccgag cagtgcccaa atcgaacgat 360atttaaatgc
tattaaagta ctttgcatag gtactggact gctgtccacc acggaggtca
420ccgtcctcgt cgacagtatg aagggagaaa atttacccca acagaaccca
gtgccttcag 480gaaatgaaaa agatgtcagt aacaaacagg aatcgtctaa
aaatcgatcg gatttatcag 540tcagcattta cgaacaatta acactaccat
taagagatgg ccgctcagta tctctcgata 600ctggcgctag cggttcctca
actgtacccg aagtgacacc tggtagcgat ccttcatcag 660aaattaatcg
tttgttttta aaggcaaaca cagaaagttt acaatcgtta agggccggtg
720acactttgat agatctgtgt cttagtttac catccatcaa aaaagccaaa
ggcaaagttg 780aagatgcttt ggccggaaaa cctttttgta tacctaccaa
tcatgcagaa gcgacaactc 840ttagaaatac actatcgtca accctatctg
acattaacct agccatgtcg gctataaacc 900taccggtctt agaaccgtta
acctcgagca aactagacaa attgtgtagc ctagctatgg 960cagttctaca
ctgcgccctt agtcaagcct cagcagctgc ggttctttct atgggctcag
1020tggtttcacc taagtgctca aatcaacagt cacaagctgg tttgaaagaa
gacgatttag 1080attcaaacgc agtggcttta gtcgaagaga ctctaaatat
gtataatttt attggaaatg 1140ttattaaaac ttctacgagg gcaggaggtc
atgtgtatca aaattattta cttgctgggg 1200catgggtgct catttcgggt
ctccaaacgc atctcacctc cagtacgtct gcggaaaaat 1260caccacatct
tagagaaagg gaggaaaaag gtagaagccc ttgtaaaagt cgagacggaa
1320atactgccag atctggatta caaaaatttc agcagtcttt tggagtactt
tcggtagctc 1380tggcttctag ggctcttagt ttgttatccg aattgtttga
cgatttatat ttagaagtgt 1440gtggtggagc agggagcata gtccaggttg
aacctgctcc cttagcaata atgggccagt 1500tcacagcatt gcagagaatt
tctaggatat tgaatgcggc tcccttaaat caccttttgt 1560tttatctagc
aatagtcagt tatagaaaag catgtacact taaaagatta catccgccag
1620agggcgacac ctttagccaa tcagattcaa cgacatatta tgaagatatg
atcatgtgct 1680ccgacgatag ttcaacggat gaagacgatg atagtgagcc
catcttagga caatggtttg 1740aagaaaccct tgcgccccca gaaacaaccg
agtccaaatc accaacttct gaaaatggag 1800aaactaaaac tgtccaaaac
gaacgatctc gatcgatggt cccggaaaaa ggagaagctc 1860acggatttat
cgctctttca actagtgttt ttgagttctt aaataaacac tttctgtgta
1920cgaaaagtac ttttatatcg aggtacgtca aaaatggctt aacggaacag
caaatgatta 1980ttttggctgc gatcattaga gacttggaca gggagacagc
gagaactgaa ataggaacaa 2040tttcagtgta tttcggccaa gcactaggtc
agctttattg cgaattctcc ggagcactaa 2100ctagattcgc acacaactta
gtaacaaacg gaaatttgag taatttacaa gcttgccttt 2160taaatcatct
aggagtatct ccctggaata ccgatgtccc tcacgcgtgg cccttacaag
2220tatatccaag gacattggct gtcttagctc aggttttgct tttgagacca
cagaacgaga 2280aagaggcatc tgtaattagt atatggcata ggttggtgaa
tacactcata gaaaatgttc 2340ttaacaatcc tcattctgtc gttgaatcag
aaaatgaaga tttaaatgtg gaacatgccc 2400aagtgctcct ctatttattt
cattcattga acttgatgca gaagaaatca gtcgtgttac 2460ttatggctgg
tggtgtttta agatgttccg agatagcaag aggtccactt aaagattcac
2520agttgctaca cttatctaga gtattgctat tattcgatta tattatgaag
cacctatacg 2580atccacctac gtcgttgtta gaacagattc aatggaacct
cttctacttg acaaacctga 2640attccgacaa agaaaaagaa aatacaatga
cccgtatgta caccgcttgg caggacatag 2700aagacaacta caggaaaata
tcggcagttg atgaattcgc catgaaacct cggttctacg 2760ttttgacaaa
tttggaagtg aacaatcaag atgctccgaa actggacggt ttggcgtgta
2820attttatctt gggaacccca gataaactac gatatccttt gttgttggat
gctctaattg 2880aaattttgaa cgtaacacac atcacttcag gtgctaacgc
gtcgaaaatg agtttcctgg 2940gactttgtgc tacacaatac tgtttcacta
tttgctggag gctattacag ctactcccgc 3000catcagcgtc atatatggaa
cgcttggcga caggcgaaaa tttgacggct ggccccttat 3060tacttcattc
tctagtctgg gggcctagag cgtcccacaa gaatttcagt agatggctga
3120aggactgtct agttaagcaa gggatgtaca cgcaacacac agacaaatta
ttaaaggaag 3180tgtccgaagc agttaacaat attaaataca atataacgaa
tgctaaaaat tgtattatgt 3240cgcttactcc tgatgtcaaa aaaggaatgg
taaccaaaga aaaccttcca ccattatggc 3300atttattttt actcgacgct
cttatgacaa acgtacaagt ggctttagaa gagcccgaag 3360ggagcactga
aagtacttca atcactgtat ctaacggaac ctacgtccag gatttattac
3420cccacgtttt aaggttaact caagcaatac tgcactgtac aagatggtcc
ttactacata 3480ctattgctga ccaaaatccg tcgtgtgaaa aattctcatt
gcagaacttt gaaggactac 3540aagatgtcct tgcaatagct tctacaaaga
atacactaac gatgtcgtta tcttctgagc 3600taaccactct tttgccttca
tgtgtaagct ctgcccagtt gcagaattat aagacaattt 3660tagaagatgt
ttcattgagt gcttatcaaa acgacataat cccatcagaa agcgccattt
3720taaaagtagt agacgcacat gtagctactc tttccatggg cagcccatac
agcatcaact 3780tgtcacttcg acgtttatta caatgcctag tcaaatttat
ttgtgatcat gcccctaaaa 3840cagaaaatac agacacgaag aacaaagcga
ttgaactact agtttcaatc actttagatc 3900taaggaccga atttttgaat
gacactgtga ctaaaacgtt agacaaaatg attggcgata 3960ccgaaataga
cgaacatcaa aaacgagtct acctgagggt tttagatcat acttataaac
4020tcataataaa ttacacgtca ggtaactcag agacgtatag tgctaatatt
gatgaaaaaa 4080tacttcacca ctgtttgaaa ttctacgaaa aaatcatcga
gaaatcttca ggcagacaag 4140ctttagaaat cttctttact ggagataggg
atttggtgaa agtgctgatg tctgtatcta 4200gtccgcaaat gtcgcaacat
tatggtacca gagtactgca tttcttcaat aaactgttcc 4260aagcagccga
gaagagctct acagatccaa gcctgaacta cttatgttca agtatgagta
4320aactggcaaa cgtagatagc gaaaagctgc aaacctggtt gcgtcaaatc
atcatcggta 4380caagtagtat tgaacccgtc gtgacaacta cagttaccat
caataaagaa gaatctcctg 4440cgacatctac caacggaaaa tggactataa
ccacggttga aagtaaagag gctaattctc 4500caacatctaa cgacgatcaa
aagtccttgg tgcaagagaa cagtcagctg ttgcaagctt 4560taactagttt
tattgttaaa caaaacagta atgtttccga agaagtttcg ataacaatat
4620tgaaggcgct gatccaactt ggaaaacatc tgttatcgcc tactctggaa
ggtactggtt 4680ttactgattt aatggtggat atgatcatgt tagcagatgc
tggttcagga aagggacaca 4740gttatttatt ccctgctaca gcagaatgga
tagaactgtg caacaaacgc atctcagaaa 4800aagatgttat agataaatta
actagtaatc ctgattcctt gaagaaaaat gctatgctgg 4860aagcctcttc
tagtattcta gactacgtta gcgatgttat atcaactatt attgcccagc
4920atcctcaaac gataagacct cttagtcctc cttgggaaag tgagtctcct
ttagagttgg 4980aatctgagtg gcaagacttc gcgaacgatg acgatgatag
cggtgaagat agcgacgagg 5040attctttatg caacaaactt tgcaccttca
ccgtaaccca aaaagaattc atgaatcagc 5100attggtatca ctgtcacact
tgcaaaatgt tagatggcgt tggtgtatgc tctatctgtg 5160ccagagtttg
tcacaaaggg cacgatttga gttacgccaa gtatggtaac tttttctgtg
5220attgtggcgc caaagaggat ggaagttgtc aagcgttggt aaagagaagt
ccccaaacca 5280acgaaaccaa taatgcaaca actggaaaca actctgaaca
tatgttaacc agttctttaa 5340gaagaaagac ttcaagtccc atcccagctg
acaagattat ttataaggag cgaaaaaatt 5400cgaacactgt caaacagtta
gatggttcaa aagaattcat catcaactac ttaggatcaa 5460gtttggtggc
aggatctctt ttagaattcc tacaatcttt aatcccagct attgaagaaa
5520actgtcgtag aaattcactt gtaggatgcc acttacgcgg tattaaggcc
ttggatcaac 5580tatactcaaa cgaaaagaaa tatgtacata ctgatcaact
tatggtacca actttaggtt 5640ctcaagaagg agcttttgaa aatgttagaa
tgagttatgc tggtgaacaa ggacagacca 5700taagacagtt gctttcggcg
catatagtac gaaggattgc gatgtgttgt atgacgtcta 5760cccaaggtcg
tagacagcac ttagcagtat cacatgaaaa gggcaaaata accgtattac
5820aactgtcagc tttacttaaa caagctgatt cgtctactag aaagttgaca
ttaacaagac 5880tatctacagc accaatacct tttactgtac tatcgctatc
aagtaatctt tgtaacgaag 5940actttttggc agtctgtgga ttgaaggatt
gccatgtact cacattcacc tctgggggat 6000cagtttgtga tcatttagtc
ttgcatcccc aattagaaac tggaaatttc atcataaaag 6060ctatatggct
gcctggttct caaaccaaat tagctctcgt aacagcagac tttgtcaaaa
6120tctacgactt agccacggat gcattaagtc ctcagtacta ctttttggta
ccgagtggaa 6180agatacggga ttgtacgttc atgtacgatg aaggcactta
tcacatttta ttaatgtctt 6240caccgggaca catatacacc gaaattttaa
atgatgattc ctctacgaag tatggttcat 6300tttatgtgac gaatacctta
gaagtttttc atttagaagc agcggatgtc attgggcaag 6360tagccggagg
tggagtatcc atctattact cacatactct tggtctccta ttctatagct
6420acgcccaagg aaaaagtttc atatctccca tcaatcccaa aagcaacagt
ctaccgttag 6480tatttgccat aaacctaccg caaaccaata acaccacttc
aaaaagcaat ggttcgaaaa 6540attctgccaa ccaaccgtta tgccaatgga
ccgagatccc aaaccatccc ggtttgattt 6600gttgcgccat gcaatcgaat
aacaatcctg ttattttgat gcttaagcca gacactattt 6660tgatacagga
gattagagtc ttaccagcca aaagtaagat tatggatatg gttgccatta
6720gacacagttc cggaaatgag ttgagaacaa ctttgattct gctgtgcgaa
gatggtagct 6780taaaaatgta tatggctaat atggatcaaa caggtttttg
gatgtcgtca agcattcaag 6840ctaacattac caactcgtcc gtaaaaccga
aaaagaagaa aataataaaa tcggggaaga 6900ctacaaattc cgtaaacttc
ccagtggact tctttgaaca ttgcacaatt ttgaccgaga 6960ttgaactggg
cggtaatgac gttttgcaaa tttataacca ggcacaactg aaaaatcgac
7020tgaacactac tggtttatac atagcttgta ataaaccact aggtttttct
ctcgatgtaa 7080ctaataatga tccgaacatt gttatggtag gactgagagt
attagttgga agtcaagatg 7140tcataaggat cccatctttt gtagagatat
ttggacgtac aattcctata acagcaactc 7200gcagccgctg gtatgacatt
gcattctcaa gagaagaagc tcttcaatca gacaagaaag 7260tcacaattct
atttggtcct tctcaagatc ctgaaactgt tactatggtc gactgtgtaa
7320aaatttatgg aaaaactaag gattcatttg gatggcctga agaaagtgac
gaaaatgtag 7380ctgctggtgg tacgggaagt tcccaacagg ctgcttccaa
ttcagatagt gatcagagtt 7440caggaaataa aactcaatta acgaaacttg
aaaagcttgt ggttggcata cttgaatcac 7500tcgatggcag cttctctctg
cattcaactg aagacaaact ggctaccttc aagacaccaa 7560cacttaaagt
tgcaacgtcc ttgttgaccc ttcccaccca gccgtcagta caaatccatt
7620ctaaggctct tttatcatct cttcactcta caaggctact gtatcataat
tacaaagacc 7680aagcgttact tcaacatgtt ttgacaactt tgacggaaat
gacaagtacc aacattaaag 7740atttagatgc cgagagctat tatagactgg
ttttgattgt aagaggaatc gctgttgcta 7800gaccacagaa tttggtgaaa
tttgctgata gccatacttc gatacaagat gttgtcctgg 7860aagatcctct
ggaaaatatt ataagagaaa agcaaggttc aacgtccaaa gaagtcaaac
7920catttgaacg taaacaacat agtcagcatc ttcttcttca gctaatggag
gttctttggc 7980tattgcactc tctgaatcct gaaattcctt cattgtccct
agtagtttca caaggactca 8040agcacaccga acaaattgtt catgcacttg
ttgaaatagt gcacgccttt aatagctgtg 8100atacatacag caatgtcact
atagcagtgt atcttcaact gttattgtgc aatgatcctc 8160tgattgcatt
tagtgctaaa caagctctta gcatagtgct taaaccaaag acgaagagaa
8220gaaaggtttt catacctagt cctcctcatt gcgtttcacc gcctcttcag
aagaccacag 8280atgataggtc taagactcct cccgctatac aacaatcggt
tagtcatgaa gaagagatgc 8340aagcaagaca cccaaatcaa tatgatgtgg
acgccattga agctattgga ttactagaac 8400agcctggtca acagcaagac
aaccacaatg ttaatgcttt ggaagcttta cttggtggtg 8460gcgtaggctt
tcctccattg ctggatattc caccagatgc tgatgacgaa gctatggtag
8520aattggcaat tgcattaagc ctacaggatc atgaaattgg aggtgaacaa
aatcagaccc 8580tgcaaagtct tcaagttcaa ttaggtcaag atgtgcttgg
cgcccaccaa ggccaaaatg 8640cgcaagcgca agaatctgct aattttagcg
acacaacagc ttcggcagct ggatcagatg 8700atgaaggttc aacagctgca
actgatggtt caactttgag gacttcacct gcagaacaag 8760ctgggtctga
aagtggcggt agtggagttg aaagtattac tggagaacac aatgtttctg
8820gaaggtcttc cgcatatgga gataatatgc aagaagcaat caatctagtt
tctagatcag 8880ataccagttc gattgctaac acggttgccg gtatggttga
ggctgatgcc cagcacgaag 8940aagttgaact ggaaactgaa agtagcagta
gattacacgt tctcaggctg caactcctgg 9000agaaactggt ggagtatctt
ccgaaattga aaaacgttga tggagtcaga gtaataccct 9060tcttacaagt
ggtactccag ttaactgcag atatcgatgg ccattctgag cgagaccgag
9120tatgtttaaa ttcactttta acaacaataa ttagtgaatt acaactaaca
aatgataatt 9180ttgctgacgt ttgcacacga accacccaac gcgaagttca
gttgatactt ttgcgattac 9240tcagtgtatt aatgcaaaga tgtaaaacat
catcaacgtc aacatctagc aaaaccccaa 9300taccagacag cactaccttt
gtttctcgca ccacagcaac agctcttcac aaagctgaca 9360ttatcaacta
ctgcaataaa ctccttcaag ctttgttgag ctactggcga aactctacaa
9420atgaggatga ttcggtcagc atcacaggaa atcttcttaa agaacgattg
cctcatccgc 9480cacctgatat gacaccattt ttccagaggc agtttgttaa
agacaatcaa gacgtattcc 9540atacatatcc acaacttttg acagaaatag
ctttgaggtt gccttaccaa gtgcacaggc 9600attcggatgt ttcagagcct
atcagtgccg cgttccatgt gtcatggtac aagcatctct 9660gtgattatat
gatgacacca cagacacctt ttgtaagaag acaagtacgt aaattactca
9720tgttcatttg tggaaataag gaaacatata ggcaattgag agacttacac
ggattaagaa 9780accatatgaa gagagtaatg gaatgctgta ccaaggctgg
ataccaacct gctactgaca 9840ttcagcatgc tttgagcctg ccgtatgatg
ccctggttga attaattgaa catttgaaat 9900attgtgttga ggtcgctcaa
agtcgcaccg gtaactggca acgcttctgc ataaaagaag 9960aagacgtaat
ccaattcttg atacgtatca gttttctttt ggatgatggt gttgcaccta
10020ccgttcttca gcttcttcaa agcgcaatag tagttaactc accggcaaag
aagcccgatc 10080catcgaaaac agcctccagg aaagatcgag aaaagtctga
cgattctagt accgaagccg 10140tgttcgaaga atctaactct gtaattttag
tagaacagat caccaagcat atatctaaag 10200aagtgtttgc gcgttttgta
aagacattta tgctagaaac taatataacc tctgtaagat 10260ggcaggcgca
tgcgttgacg ctagccattt ataaaaactg taaacctaaa gatcaagaag
10320ccatactaga ccttttgtgg cagttatggc cattgctacc ggcttatggg
agaaaagctg 10380cccagttcgt cgatttgctc ggttactttt cactgagata
tacggagaaa accgaaggtg 10440ttgaagctat tcccgaatat gtcgaacgag
cattcagtgt tcttaaagca cagaacgaaa 10500tgttagcaca tcacccaaat
gcaaacctat atgctcacat gggccaattt gtagatctgg 10560atggatatta
cttggaatca gaaccttgcc ttgtttgtaa caacccagaa gtttcgttta
10620ctgtcattaa attgagttca atcaagattg attcaaagtt tacaacaact
acacaaatcg 10680taaagctact gtccagtcac acaattagta aaatcactat
aagaatagca gatttaaaac 10740gaacgaagat ggttagaaca gtcaatattt
actataacaa tagatctgta caagcagtag 10800ttgagcttaa aaataaacct
gcactatggc ataaagcaaa gaaggttact ttacaatctg 10860gtcaaactga
agttaaaatc gagtttcctc taccaattgt agcctgcaat ttaatggtgg
10920agtacgcaga tttttatgaa aacattcagg cttcatccga aacgttacag
tgtccgagat 10980gtagtgcctc ggtaccggct aacccaggag tatgcgccaa
ctgtggtgaa aatgttttcc 11040aatgtcacaa atgtcgtgct atcaactacg
acgaaaagga tcctttcctg tgccatgcat 11100gtggattctg caaatatgct
aagttcgact ttagcttact ggcgaaacct tgttgcgctg 11160tggagcctat
agaaagcgac gaagatcgca aaaagaccgt ttctagtata aattcttttt
11220tggaaaaagc cgatagagtt tacaaacaac tcatcgcgaa taaacctact
ttggaatcgc 11280tggttgttaa gattacagag cacagacccg ataaaaaaga
agatgctgtt tcgacttcgt 11340cggcaccgac tgcaggtggc gctgcagcag
cagctgctgg tgctggagcc cagttgaaag 11400tgaacaaaac aatacaaatg
ctggctcaac aatactgtaa tgagtgcaag acttcgtttg 11460aggagctcag
caaaattata cagaaggtgt tggtgtcgcg gaaagaattg gtggcttatg
11520acaggaaaca cagagatatg gaattgccga aatctacacc ggtccttagc
gaaaatctgg 11580tcaattttcc ttgcgttaac aatcgttgtt acggttgttc
caccgctgcc acagaacact 11640gcttgacttt gctaagagca ttggcacaca
atcctacaac cagagaagtt ctatgttcac 11700aaggactcat ccaagaacta
gtatggaaca atctaagaaa tggaagcatt cacagtcaag 11760aggaggtgag
acaattactt tgcgttctga ctaaggataa caggactgcc acggaagaac
11820tctgcaacct tttaatggag aggataagtt tgagtttgaa tggccacatc
agttctgcag 11880acttgggtac gagtgttagg catgaaattg ctctcttggc
agcgatggtt caaaaagaag 11940atgactgctg ggagctgaaa ctcaggtgta
tgatgaatct gttcctgaaa gcatgtgaag 12000gatcgagaag tcccttggtg
atggaatcta tcattttacc atgtttaaaa atactgcaaa 12060gtttaatgaa
aattccggaa cctggatcta agaaaactaa ggaaaaacca gctccttcct
12120tgcaactaag cgtgcaactt gaatctccgt atggtgcgat ggttgacctc
aataaatttt 12180tggataaaga cgatgctcat acttttaatg ggtggaaatc
aagattccct aaggcacaag 12240aggcctctgc ctcggccaaa ctcagcaaag
aggagaacag aaaatactat ttggctgaaa 12300aatactgccg caaatggaga
agacacatta agaacctata tatccgcgac ggactcaatt 12360tcgacgatac
ctcatggctc aaagccgtca ttttcaaccc aagttctcga ttagcccgac
12420aagtcgcatg taacatcata gaaatcatgt gcagcagttt cgaacgcaag
cgcgacatgc 12480tcgatctgct aactcatttc ttagcagagt taaatactgc
aggcgaaagt gctgccgagt 12540tccttgccat ttaccaagaa cttatcgcgg
agacgccttg gaaacagtat ttaactgtta 12600acggcgtact tacagtgctc
gcgcacctta tcaccatcga gatagagcaa cttcacaggc 12660tcgaagagac
aactttgacg tcagatcttg cacaaggata tgcattgaat cagattacgg
12720agttgctgtc atatttctta gatgacgtgg ctattagaag acaatacaaa
agcagacttg 12780ttggagcggt gttgaatgga tacctgtctt taaggaggct
tgttgtacag agaactaggt 12840tgatagatga tacacaggaa aaattattgg
aactactgga agaaatgact acaggtacgg 12900aagaggaaac caaagctttt
atgtcagtgt gcattcaaac agtagaacga tacagtcttc 12960aagacatttt
aactccggtg ttcatctttg aacgtctttg ttccattata tatcccgagg
13020agaatgatgt aggagagttc ttcttgactc tggaaaaaga tcctcaacag
gaagatttcc 13080ttcaaggccg aatgttaggc aatccttatt gcagtctgga
agctggattg gggcctttga 13140tgagggatgt caagaacaag atctgtcaag
attgcgaatt ggtggctctt cttgaggacg 13200acaatggtat ggagctcttg
gtcaacaaca aaatcatgag tttagattta caagtgaaag 13260atgtatataa
aaagatctgg ttggcagaag gaagtgacca tgaaccgatg aggattgtgt
13320acagaatgag ggggctcctt ggtgatgcta ccgaagaatt tgtagaaagt
cttagtaata 13380aatcccaaag tgatgtagat aatgaagagg tctataaaat
ggccaatgag ttagccgatt 13440gtggaggact tcaggttatg gtaactagat
tgggagcgat accgagcgtc accagagcta 13500gaccgttgtt acaagtgtta
ttaaaattat ttaggttatg tgtgaaagtt aatcgttgtc 13560aagaggtttt
aataaagccg gagttgaagt ccatggaagt atttttacgc acgttgcaac
13620tttgtttaga tagcgataaa gattctagtc aaactggcgt taccgaacaa
ctattagata 13680ttatggaaac gatcttgtcg aaagcaacga gtgaatccga
agaaaacttc accgagttct 13740cacaaactct aggtagtgcc gaatacgtca
aatctttact gtcatgtact aatcagcagg 13800tagtgaagaa cagttccgtt
ctagtccatt taaccagggt attagccgct ttggtttacg 13860gcaacaaaga
gaaaatgaag attttacttg accatttcag ttccgtcctg gatttcaaca
13920aatatgacat ggaacataac tcagacgatc aacaaaaact cgagatgttc
tgtgttttaa 13980ccaacagcat cgagaagaat gccatcggca gcacgttaaa
agattacata atcgctttgg 14040aaatagtcaa aaatgcctta gaatacataa
ctatgcacgc accttgtgtc aaaccgactt 14100tattaagggt agacagcgac
gagttgaaag atttcatatc caaaccggct ttgaagtaca 14160tcttgagatt
tttaactgga ttagctcata gtcacgaaaa aacgcaacta gcaatagctg
14220ctgcggaaac tattccgatc attcatcgtc tggaacaggt gtcttcagat
gaacacgtcg 14280gatccctagc tgaaaacttg ttagaagcac
tctgcacgaa tccggatgtc gcaaagcaga 14340ttgacgccgt cagagagttt
acaagatctg agaagaaacg tctcgccatg gcaatgaggg 14400aaaagcaact
tggtcagttg ggaatgagaa ccaacgataa aggccaagtc actgcaaagt
14460ctaatatact ccaacaaatt gaagaattgg gcgaggaatc cggtttggtg
tgttgcatat 14520gtcgtgaagg atataaatat caaccaacca aagttttggg
gctgtacacg tttacaaaaa 14580gatgtaatgt tgaagaattc gaaggcaaac
caagaaaaac tgttggatac actactgtca 14640gccacttcaa catcgttcat
atcgattgtc atatgagtgc agtgaggttg gcacgggctc 14700gtgacgaatg
ggaatctgcg gcccttcaaa acgccaacac caaatgcaac ggactcttgc
14760cgctatgggg tccacaagta cccgaatcat catttgccag ttgtcttgcc
agacacaaca 14820catacttgca ggaaagcacg aatcacagag atataggaca
caattcaact attcacgatt 14880tgaagctttt gctgttgagg tttgcacaag
agaaatcttt tcatgaagat acgggaggcg 14940gtggaccaca aagtaatatg
catttaatac catatctgat acacgttgct ttatatgtga 15000taaatactac
aagagtgaat aaaagggaag aagccaacct tatgtcgtac atcgaagcta
15060ctaatgcaga aaaatggata gaaacagcgt atgatgctga aggtcctcta
tattggataa 15120cgatgtccat tttacttcac tccagacaac agtggcaagc
acatcggctt tctcacttca 15180aacggttaat ggttttggca caagtgcgac
attgtcaacc gtccggacct gtcaaaactg 15240tatcagacaa atcagtaaaa
gattataata tctataaacc gtacttgttg ttctttgggc 15300ttatcgatgg
aatctataac catttcttca agaatgtggg aggtccagac gaacaatggt
15360caaccaactt agcagactac attcgccaca atgatgaagc tttgatgaaa
tcttcagaaa 15420agctcttggc tgattataca gatgaatttt taccttgtac
ttccttttca gaattctgtg 15480atgtggcagg tttactcgac attataacca
cgccggatag gtatttaagt gacctgctga 15540atggattacc ttaatgtgta
taaaatgttt ccgaatttta aaatgtaaac aaaggctgta 15600cactgttttt
actaatcgct tgctgctagg ttgtaatcta aataaatgta gtatatattt
15660gcttgttgtt ggagttttag aaaaactaaa ttattgtaat tacgattgat
taattattat 15720ttgaattaaa 15730114852DNADiabrotica virgifera
114aagaaacaaa ttgaaattga aaataaacaa aaataaaaaa taaccagtcc
aaaattaaat 60ggcaaatatt acaatagtaa aaaacaaaat atgaatgtgc tccagtctca
aaacgttgcc 120agagagatga tgttttacgt aagaccggtc tgactgttgg
aaaggtagat ttatattatg 180gaagaggcgt ccgaaatgat ggatctcttg
tacccccgat tagacaaact gcaagtattt 240ttaaacagcc cgtaactgtc
tataagctac aagagagtaa agtaaaaaat gattataaaa 300acgggaatca
agagaaacca aagcaactaa tttgggagaa aagattggaa ggtttaaggg
360cttgtgatat ggatggcttt gagttcgaag ctatggaact gccaaaaggt
ttaaaaccag 420tgggacctaa tgtaactgaa gacaccatta tccagagtgt
tgctacagca ctccatgttt 480cttctcaacc agtgacaggc cagacaagta
caaaaacact acttgaaaaa aatccaggag 540tatttctcga tcccaaacaa
ccattagtac acgctgtaaa tatatcagag gatgatatca 600aacgtcaaga
ggagagagtt gcattagcga ggaagaagtt acaagaggct ctaaagggca
660acctataaga tataagtatt ttgagtaatg ttttatatgt acatgtcata
attattagat 720tgtaagttac aaattaaatc gttccaatcc tgtaaattat
aaccaattgg taaataaaat 780aagtttcttc agtcatatac aaaaattata
ctttttaata ataagaatgt caaagacttt 840atatgtgtga ac
8521151099DNADiabrotica virgifera 115ctaacaaatt tatacgtata
gaaatatacc ctgtgatgag agatgttagt ttagacaaat 60agaacaacat agggataaaa
ctcttctcgc tccggggtaa cggcagagct ttcacaaccc 120gaaatgttac
ctcggttaaa ataataataa tggcaccctt taaaattgtt atggttcgtc
180atggagaatc agaatggaat gaaaagaatc tattttgtgg ctggtttgat
gcaaatttaa 240gtgaaaaagg taaacaagaa gccattaatg ccggaaaagc
actaaaaaat gcaggataca 300aatttgacat tgcatataca tctgtcctta
caagagctca gaacacactt aattcaataa 360tcaaagaaat tggccaagag
aatttggaaa ctataaaaac ttggagactc aatgaaagac 420attatggtgg
cctcactggc ttaaataaag cagaaacagc agcaaaatat ggagatgagc
480aggtagctat ttggcggcgc agttttgaca ttccacctcc accaatggaa
cctgaccatg 540cttattatga taccattgta aaagatgccc gatatgctga
tggtcctgca ccagatcagt 600ttcctaaatt tgaatcctta aagctaacaa
ttgagcgtac tttacccttc tggaatgaaa 660ctgttgttcc acaaattaag
gctggaaaac agatcttaat tgcagcacat ggtaacagtt 720tgagaggaat
tgtaaagcat ctagaccagc ttactgatga ccaaattatg cagttgaatt
780tgccaacagg aattccattt gtctacacat tagatgaaaa tttgaaacca
ataaagagtt 840tagaattcct aggagatcca gaaactgtga aaaaggctat
ggaagctgta gctgcccaag 900gaaaagccaa ataagcatta tttattattt
attgttttaa tttatatcaa aatcatttat 960tgttagatat ttgatgtgta
atgaataaat ggttaggctg aattgtaaaa ctcagcagaa 1020atgttatgtg
caagacatta aagcatattc ttagataaga aaaacatgtc tgaggatgat
1080aaaatataaa aaaaaaaaa 1099116523DNADiabrotica virgifera
116caactagcgt gttaagactc tcttggtctc ctgatggtca gtatttagtg
tcagcccatg 60ctatgaatgg tggaggacct acagcgcaaa tcgtagaaag agaggggtgg
aaacatgata 120aagattttgt tggacacagg aaagctgtca cttgtgtgag
gttcaactcc aacattctcc 180aaaaacaaga aaagaacaac tctaaaccca
ctcaatattg ttgttgtgcc attggatcaa 240gggacagatc tatcagtgtt
tggttaactt cattaaaaag acctcgtgtg gtcataaaag 300acttatttaa
taatagtgta ttagatatgt catggagtag caatggactc tatttgatgg
360catgctcttg ggatggttct gtagcatgta ttgtgtttgg acatgcagaa
attggaacac 420ccttgactat ggatgagaag aatgaattat atgaaaaaaa
tgtaccacaa atcatttcaa 480aaaaagttgg aatcgtaatt ttggtacgtc
ccaaatcatc gaa 523117531DNADiabrotica virgifera 117attcccaaga
aaatagaacg aaatctggtg aaacatggat aaggataatc acccaaggtc 60aggaagtgac
tattgactgc gatgaaaaga ctttagcaga aatgggattt aaagataacc
120agatcgttta tatatcgata ggcatgtcga ggaatatgaa gaaacgggag
tttatggatg 180caccatcttt acacccgcct cctcctcggg aatgtttacc
aacattactt cttctacagc 240caaattactt tgagcagcta ttttccttaa
tgcacacact tagttcaatg aaaattcaaa 300ttaaaggagg gtgccaatta
ccacatacaa gagcccaagt tctcagcaga agagtatggg 360atattcttag
cttattacca accagtccca aacttcttcg tggtttcaaa tacttggata
420tccctcttcc agaattgtta gatccttcaa gtgctcaaaa attaatgtat
tctttgtata 480ttgtcgaatc tttaagcgcc aaaatgtacc gaggaaagta
ttcagatgag g 5311184324DNADiabrotica virgifera 118gtagaaaagg
caactgtcac aatcgatatg agaagcgctt tcaaaaagtt cgtagacact 60agattattgc
atatgaaggt tttaccatcg cctactagaa cagctttacc caaatgcgaa
120ctgtgggatc cagaaactat gaccagtgca ctcgatgtcg taattagagc
agcaaaacac 180tcgtatcccg agccagtaat gttagtgaga ggttttagcc
agccagtcaa agttagtttt 240gtgtatagtt gtaaccgatt ctatgttcag
ttagtaaaaa aagaacagga actaacaact 300cttatggaag agcttcaaag
tttatgtcta gaaagtgctt ttatggactt ttcttccgtt 360aaaccaggaa
ttccatgttg tgcccaatac agcgaagacc agatgtggta tagatcgcaa
420gttacagaag tacaaggaaa tgtagtcaca gttcgctaca tagactacgg
aaacgaggaa 480tcagtggcta tagaccagct gaaaaacatc gaaggcgaaa
ttctaactgt tcttaaacct 540caagccattg aatgttgtct gaatggatac
caaaagatgg aacctgatct ccaaagggat 600ggtttacttg aggaactgct
attggaaaac acatttacaa tgaaagttgt ggatatgtgc 660gacaaaaagg
cattggttga cttatttgat gaaaatgaat acaacgtttc ttccttgttg
720ctggtcaaat tatctgaagc tagttcacag gtcagtcccg tattggtaca
agcaggcaac 780aaactagaac atagaaaatc atatacacaa caaagggaac
agaatttcaa gggggacaga 840agcccaaaca ccggacgtga acaaaaacct
tgggaaaaac ctggagacag gaacgaaaag 900tcttggagac aagatagtcg
agataataga gataatcgaa atttcgaaaa aaatacgaac 960gacaacgata
gatcttggcg tcaaaataac aaagattaca gtgtaaatag tcgactggac
1020aaatacaaac aaaacgatac cgggaacagt aacgactata acagtaacag
taacgatgcc 1080aattggaatg acaatcagac cagcgagagt tggaacgacg
gcgccgaaaa taatcccacc 1140gaagaaccaa aagacgaatg ggtaactggt
tcgaacgata acaatgaagg tggatccagt 1200tggagaaaca acgataacag
aggcgataga aagaacagag gggggtctca tagtgacaga 1260ggtggtagag
atggtttcaa gagagacaga gatggaggtg gttataacaa cgatagaggc
1320ggtcgtggag attataacag cgataggggg gatcgtggag gatttaacag
tgatagaggc 1380gaacgtggag gatttaagag tgatagaggc gatcgtggag
attttaacag cgatagaggc 1440gatcgaggat ttaaaagcga tagaggcgat
cgaggattta acagtgatag gagttataac 1500aacgataggg gcgacagaga
cgattttaac agcgataggg gagatcgtgg aggatttaga 1560aatgatagag
gaggaagagg tggaggacgt ggaggtttca acgatagagg aagccaaaga
1620gatggaggat ataaaagaga ttataataaa aaggatggat ctgaggatgg
ttctgagaaa 1680ggcttccaaa gaggaaacag atcgaattcc cgctatgatc
gaggaggtag accccctaga 1740gacttcgaca aagccaggaa tttcgatgac
aacaatgctg gtagagattc atggaactcc 1800acaccgaaag caccggccat
agattctgtt cccgtctcta ccacattcca gtcatacgaa 1860gtcgttggta
ctgaagcgaa gattacagtc agttggttcc ataatcctga actattccat
1920tgccagttag tagactatca agaaacattc aagaccatga tggaagaaat
ccacgacttt 1980tacaaagaca gaaaacccga attggtagtt tgcggtgcac
ctgtagttgc tctgtttccc 2040gaagacaatg ttctgtatcg cgcaagagta
ttggaagttg taggtatcga ttacaaagta 2100tatttcgtcg attttggcaa
cgtatccata gtttccaaga cgtttcctat cgataagaag 2160tttatgaacc
ttcctgctca agctatagca tgttccttga acggcgtaag tcccgttggt
2220gaagattggg cagatccaga tacatatagc ggatattttg ataaggacgc
ttttgtttgc 2280aattttatta aaaccgttga tgaaaaagct tatgtaaata
taacctacga atcccaaagt 2340atcgctgacc tattagtcag agacggcttg
gccctgtcgg ccgcatctgg tccgtcggac 2400ggagatgtag atatcaacgt
gctactcggc cagcaattta gagctactat catcagcgta 2460aatgatctga
acgacttcat cgtgggactg gattgtggta aaatcatcac ctgcaccatg
2520cataacttgg aaactgcgac tgaaactttt aaagacgacc ttaagggtct
cttgcaacag 2580gctgtgatta tatatgtcga taatgtctta gaagataata
agttagaaat taccctttat 2640gatactgaag gaaataaaca cgttatagtt
aaccctgatg aaggtgcata cgacacttta 2700gatcttcctt gtccaacgct
gatattacgt tcaaacataa ccggttacgt acctttcggc 2760agcgaatcat
cagtttttat ccaaccaaac gaatacctcg aaaccataaa ttcccttctc
2820gatctgttgt ttgaaactta cgacagtaaa ccaaacgaga acacaataat
tcccgaaact 2880gattccatat atgcggtaca tagtgaagat ggaaactggt
atcgaggcaa agtgactgat 2940ttcgatgatg aactggctac ggtttcgttt
atagattatg gcaactctga gcaagtggcg 3000ttttctgcgt taagagagct
tgaaaaagca tttttggaca ttcctatttt gtgcgttgag 3060gttaatattg
gcgttgacgg taccccgttc gttgacagtg aagtaacagc caaagtcttt
3120tatggtgaaa ctggctggga aggtactctg cagcccccgg agtcttctgc
tccagaggcc 3180gcttcagtac cagaaccgca ggaaaattat gtcgaaagta
cacaggaaac tgttgcctat 3240actgaagaga taccacaaga gaatatttcg
aatgatcaac cattacatgg tcaaatagtt 3300gaaacaggat catctgctga
agcagcatca gctgttgatg aaccattaaa aggaactgtt 3360gtttatatga
gtcatattga ctccccaagc gatttttacc tgcagtttgc caaaaacaag
3420gaagatatcg aaatccttca aaatgaatta caaactatgg tagaagaaat
ggaggttctc 3480gaaaatccag cacatggtgc tttgtgcgct gctccctatt
ccgtggacca atagtggtac 3540cgagcagaag tgttggatgc tgatgaggat
attaccacgg tgcgattcgt tgattttggt 3600aacactgatg tcatcgataa
taacacaaca aaagtaaaaa ctcttcctcc aaagcttcta 3660tcactagcga
tctacgcaac gagatgtgcc cttaaagttg agcccttggg agaggaatgg
3720gacgctttag caatgacagc ttttgagaat cttaccaata tcgaccttaa
tttaacggcc 3780gagattataa atcaggatga aaaatgcacg tatgtggaac
tgtacagtga tggtacggat 3840gttcgggcag ctttggtgaa tgacaacctg
gttaaaccaa ttgcagaaac gaccgaaaca 3900agacagactg gtttcgtaag
ccacttaaac tcaccgtccg aattctggat ccaactggag 3960agttgcatag
acgaactcga atggattgcc gaacagctca gctcagctga gacattcccc
4020gacgtaacgg atttgactcc cggcacttta tgtgcagctc ttttcccaga
cgatcaaatg 4080tggtacagag ctagaattct atctgacact gtggcaggtc
tagaacttat tttcatcgat 4140tatggcaact cgtgcgtctg cacggatttg
aagcagcttc cagaagattt ggtcatgact 4200gcgccacttg cgatgaagtg
tagtctgcag aagaacgacg gtattcccac atggacgcca 4260gaggccgctg
ctaagttttc ggatatatcc gccgaaggcc agacgatttt taccgttaaa 4320aaaa
43241197780DNADiabrotica virgifera 119aaaaaataaa agtgtcgggc
gataaaaagt cattttatgt actttttaag acgtcctttc 60ctctagaaac ccacaaagtt
ggtcaaatta tgagtataga gttgaaccta caatgttaat 120gtaaaaacga
atcaaaatat gactattgca actagggcac aaggattgac aggagatagt
180gttgaacctc aaggatcttc tcctggacca tctgagaaca acgtcgtcga
ggaaatccaa 240gatgaaaact tcgaaccgga gttccccaaa gataaattag
cgtcactgga tgaaaaaata 300tcgagcttgc gatgggtggt gccagtcctg
gccgaccagg agctagaatg tctactgaaa 360gtctccatag acttggcccg
aaaaaatctc gatacaagat ccgaatcttg ccagaggttc 420ttcagggagg
gattgacggt gtctttcacg aagatattga cggacgatgc agtgtccagt
480tggaaaccga atattcacgc ctgcatttat caaaactgcc tgaaactcat
agagctgtgc 540gtggtgaagc tgcctcaaga ctggtaccct ctattggatt
tactcgccat ggttcttaat 600ccgaataaca agttccacag cttcaattct
tctagacaga gcgaaacggc cggtccgaat 660tgtaatttat cggaagagga
gctcttcgct agaccatcga gcgacttgag gaatcccaga 720gggtggttgg
tggaccttat caacaaattc ggagagttgg gcggtttcca agttttatta
780gatagattcc aatcggggaa aaatttaagt atttctatag tgttcgcttt
actgaggcct 840tttgggtttt gctttgaatt tttgacggtt cacagtatca
acaagtatat attgcctata 900ttagaaatga ttcctggtat attagataaa
ttatcggacg aagaattgaa gaaggaagcg 960aaaaatgaac aaaaaagcga
tatcgtctcg gctattataa aatcgtctaa gaatttggca 1020tctagagtac
ccaatcaaga agaacttatt agaactttag aaggatttag attgactatg
1080atattaagac agttgcaaat ctccagcttt aacggaaaga tgaacgcttt
aaatgaaatc 1140aataaagtta tttcgagcgt tatttattac ccgaatcgac
accacggaat ggaagaggag 1200gaatatctca cgcctgaacg tatggccaag
tggattaatg aaaataaagt attagaaata 1260gtgttaaggg attcgctaca
ccagccgcag tacgtagaga aactcgagaa gatactgagg 1320ttcgtcatca
aagagagggc gctaagtttg cacgatttgg acgccgtttg ggcagctcaa
1380gtcggaaaac acgaagccat agttaaaaac gtccacgatt tgttggctaa
actcgcttgg 1440gacttcagcg ccgaacaatt agaccattta tttgagtgtt
tccaaaacaa ctggacttcg 1500gcgtcgaagc gacagagaga aagactttta
gaactgatac gtcgattggc ggaggacgac 1560aaagacggcg tcatggcgca
caaagttttg acgctgtttt ggaatctggc acacgccgaa 1620gatgtcccaa
ccgaaataat ggatcaagct ttagctgctc atgttaagat attggattat
1680agttgttcgc aggagcggga cgcccaaaag acagtttggt tggataaatg
tgtggaagaa 1740ctgaagaacg gagaaatgtg ggcgcttccg gctctcaagc
agatccgtga gatttgcacg 1800ttatacgaac agaacacgaa cggcagccac
actcaacgta cccaccacat ttactacaga 1860caagaagtga tagaacgatt
gcaaaaccag cattctctag ttatattagt aaccaacagc 1920ctttgtagtt
atatggatag attaagagaa ttaatcaaag agaacccgga attgaccagc
1980gagacctaca tacccgacgg acggtacggc cacacgctgc aagtccagga
aagactgaat 2040tttcttagat tcctcttaaa agacggacaa ttgtggctat
gcgccgaaca agcgaagcaa 2100atatggcagt gtctcgccga gaacgcggtt
ttccagagcg acagagaagc ctgtttcaag 2160tggttctcta aattaatggg
agacgagcct gatctagatc ccggcatcaa caaagacttc 2220ttcgaaaaca
acatattaca gatagatccg atattgctca cagaaagcgg gatcagatgc
2280ttcgaacgat ttttcaaagc ggttaacgtg aaggaaggca aagtgaaata
caaaagaagg 2340tcccttctaa cggaagatcc cgatctaata ggtctcgatt
atctatggaa agtagttact 2400ttgtgcccaa acgatatcgc taccagagcg
atcgagcttc ttaaagaagt cagtaccaac 2460ctcggtccta gattgaccca
agcgcagttg gtgttccacg aaaactacat caccgaatgc 2520tacgacaggc
ttcgagctca ctacgacacg ttaagtatct tgcaaaaatc gaccaacgac
2580aaagaattcg actccgagca acttcaaaac cgcattaagt cggaagccgt
gaaaatatgc 2640agggtactga aggttctaca cgagtacatc agcgaatgcg
acaacagctt cgtgggggaa 2700cgtaaaattc ttccgttgca cagagcttgt
tacggcaaac acattactct gatcgtgcgg 2760ttctctagtc ccaacaggca
agtcgaagac ttggaactgt acacgcattc gaacgatact 2820ctagcctctt
tgagaaaata cattttgaaa cgaatcaagc ctggtatata ttgcaagctc
2880gaattgttca tcaacggcga gcctttggag cccgccgagg acaagaaact
tctttcgcag 2940atacccatca gggacaaaat gttaatatca gcaaaaatag
ttcaaatgaa ctcgaatatg 3000gcgtcgtccc aggacagtag ttcggacagt
tcgacctctt ccccccacca cccgtacgac 3060ggtccaaacg tcgaagcgga
gaatctgtta ccgggcgttc tgatgtccca acaaccgttc 3120tacgcccaat
tcttctgcca gttgatgaac gtaggaagca cgttgccgtt tgcgatgttg
3180agagatatgg ggcacgcgct tcttcagttg atgccttgca atctgatgac
gatggagaaa 3240ctgaggattt tgttttctgc gccgggagag cagaacattt
cgatggatag catgtttttc 3300agcgcctcgc cttctgaagt attatacaac
ttggaagtcc tctacactat gttaatgcca 3360gccatagacg ccatctccga
aaaatcgtac gaattccagt acagcttcat gatatccgga 3420gaagcccacc
tcttcctcga gatgttgacg aagaataact tcatgtcgag cgccgataac
3480gttaccagac gttcggcgta tctagttgtc ctgaaaatat gtaaattgat
actgacatct 3540gtcgcgcacg tcctggtaag actgagtgaa gatcacactc
ctccagaaaa tgaggctttc 3600aacgaaaata ccactccacc gggaaccttt
ttgagacagg cattacgaaa tgttcctgga 3660cactcggatc atttactgcg
acaagtttcc atgaaactag ctcaaggttt agcgcaattg 3720atggtatccg
aaacgggaaa caccggccaa gctcaagcac tgttcagtca agcgttgaat
3780tgggaactgc cagacttagc cacgacgttg gctttggtgc ggctggtttg
ggcagcgagc 3840agcaacaatc tcggcgctgt caacgcctcg ttggaaaact
tgcactcttt atccgaagcc 3900agcaacaaga aaaagtcgga actagtcaac
gacgacgtgc tcctctgcaa agaagctctg 3960gaactgctca gtaccgccgt
agtgctcaac cccagcagtt tgcagcactt gtaccaggac 4020aattggtggc
cttacttcgt tacggacttg gtgctagtca atccgaattg ttccataaga
4080gtcgcagctg ccgaacagat gatcattata tgttcttgcg gcgctagcag
tcaaatggct 4140ttgcacgtca tcatgccgtt gctcttttct ctcatcaata
ccatcgtagt cgaacacgcc 4200aacacgtccc acgagttttt tcaactgttg
tgccgtttag tcaatgtagc gtaccttacc 4260ggatgtccca tcaacggtgt
ggagacccta ctgtcaaatg aggttgcgtg gttacgaaaa 4320gccagagaca
aacacgaagt actgatagaa ggccatctcc ttctcgccaa agagctcctt
4380ttgctgatct cctcagaaca gaagtgcgaa ctgggtagtg ctgagtccgg
cagtctgatt 4440aaagaattgc tcgaagactt cctgtttccc gccagcaagt
tgatgttgag attacacaaa 4500acgggtcagc tgggcgaaga cccggccatt
cctatttgtg atactcccca aactcaggcc 4560gcagcattcg atctgttggt
atcactgtgc atgaactgtg tacaaaacta caaacagctg 4620gttaatatgc
tcacggagat gttttacaac gatcctgaca ctgcaatctc ggaatgggac
4680taccttccgg cagtagggcc gcgtccattc caaggtttcg taggtttgaa
gaacgccgga 4740gctacttgtt acatgaactc agtcttgcag caactctaca
tggtcgacag tattaaagaa 4800ggcattttgg cagcagaagg agcagctacg
gaccctaacg aggatttcac cggtgaagag 4860agactggata tggacgtgga
ttgcactgat gataggaata gtttggacga taataggaag 4920gactacaacg
ttgggatttt gaagcaggtc caggctattt tcggccattt ggcctgttct
4980cgccttcaat attacgtgcc taggggactg tggagacatt tcaaacttca
aggcgagcct 5040gtgaatctga gagagcagca ggatgccgtg gagttcttca
tgtcgttagt ggagagtctg 5100gacgaagctt taaaaacatt gggacacgag
cagatcatgt cgaaaattct tggaggttcc 5160tactccgatc agaagatctg
caaaggctgt cctcatcgtt attccaaaga agaaccgttt 5220agtgtgatta
gtgtagatat taggaaccac agtagtttgc cggattcgat ggagcaatat
5280gtcaaaggtg aattattgga aggcgccgac gcttaccact gtgaaaaatg
cgcgaagaaa 5340gtagtgactg ttaaacgact gtgcgtgaaa aaacttcccc
cgattctagg tatccaattg 5400aaacgattcg aatacgattt cgaacgagta
tgcgcgatca aattcaacga ctacttcgag 5460tttccgcgcg aactcgacat
ggaaccgtac accgtgtccg gtttggccaa aatcgaaggg 5520gaaattatag
actgtgatct tcaacctacc tccaccgagg tctgtaccaa gtatcgtcta
5580tctggtatcg tcgtccattc tggacaagct tcaggtggac attattattc
gtatattaga 5640agtagagatc cctcgggaga cgtcagatgg tacaaattcg
atgacggcga cgtgtcggag 5700tgtcgaatgg gcgaagacga ggaaatgaag
gtgcaatgct ttggcggcga ttatatgggt 5760gaagttttcg atcccatgtt
gaagaggact acgtatagga gacagaaaag gtggtggaac 5820gcctatatgt
tgttttatac caggcatgat gttgaggagg agagtgcctt gaagatgatg
5880aaccaattga cgatttccgg tacgagaaaa
gaaacacatc tcaagatgcc gatagccata 5940gaaaacagta taagaaagca
aaatatcaaa tttctacacc ataggagtca attttctttg 6000gaatatttca
cgttcatcag aaaactggcg acgagttcag ctcagggcaa tactagactt
6060agtcaaccgt tgccaaacga tcaattagaa caacagtatc tactcagtgt
tcaactagtc 6120agcaaattcc tttttcatac tgggtggcat acgaagaaga
atctacgagg acctgccatg 6180gaatggtgtg acgtcctatg tttgcatctg
cgcacctcgc ctgtgatacg ttcctggttc 6240gcacactgca tgctcttcga
gcacatgagc cgtttctgcg aatacctgct gagttgtcct 6300agcaacgaag
tccgttctgc atttatcaag atcgtggtat tgctcgcgca cttctccatc
6360aacgacggac cgtcggcttc tccggcggcc ctcaacagca ccgccactgg
ctctttgagt 6420gatcacatcc tgtgggcgtt gctcagtctg ctgcagaggg
aggtcagcga gcacgggcgg 6480catttgccgc actattgtac ggtgtttcac
atgtacgcca atcaaggtat tcaagaaaag 6540acgcagctgc tgaggatgaa
cgtgccggcg acctttatga tggtcgctct agacgaaggt 6600cccggaccag
ccataaaata tcaattcacc gaattaggaa aacttaatca attagtcgct
6660tgtttaattc gatgctgtga tgtaagcagt aagtgccaga acagtaccaa
cggtccagtt 6720ttacccaatc cctacaaaga tcccaccata caggaataca
tcatgccaat ctcctcgcct 6780gccgccgaaa tcctattcaa caggaacgta
tacgtcaaga aagtgatcga agatacgaat 6840ctgactgaag acgcgatcaa
attcttgcag ttctgttcgt gggaaaatcc tcatttctcc 6900agggcggtat
tatcggagct cctgtggcag atagcctacg cctattgcca agaacttaga
6960caccacatag agatcttatt gtcaattttg ttaatagaag attcctggca
gaatcataga 7020atacataatg ccattaaagg tgttccggaa gagcgggaag
gtcttctgga aaccatcgtc 7080cgtgccaaga atcactacca gaagagggcg
taccagtgca tcaagtgcat ggtggcgttg 7140ttcagtcgtt cgtcggcggc
tcaagctatg ctgttgagac aggccgaagt gaggcgatcg 7200tggacctcag
cagtagcttg gttacaggac gagctggaga ggaagtaccc cccaaatgct
7260cagtactcct acaacacgtg gtcgccaccg gcacaaagca acgaaagctc
caatggatat 7320tttcttgaaa gatcaaacag tgcccgcaag acgttagaga
aggcgctaga actgatgcct 7380gagaccgaaa gagaagagga agtggaagag
acgcactcgc aagaggagcc gtcgccgccc 7440tccgagcagc attcgcagca
gcacacgatc gcgcagccgc aaaatgtcga taacaacgcg 7500ccgtcagagc
tgccggatgt aacacagcac tgcgaaaacc agcaggacag gtaaaagaaa
7560tgacggacgg gacggcggtt gtcgagcatt tcatccactt ctataagctt
tgcacgttat 7620ataaaacggg cgataggaat gtgatatgta tgtatatatt
ataaaaaaaa aaaaaaaaaa 7680caggttatat tggctgggaa gcatttttct
cttgagcatg ttcctcatat ttttttggtt 7740tttaatccgc aagtctaaat
gtaaataccg aggcaaaaaa 77801201219DNADiabrotica
virgiferamisc_feature(1198)..(1200)n is a, c, g, or t 120gtttggttta
catcttagtt tttaaaataa aaaaatgttt gaagctcaag aaattcacgc 60agaacataaa
gatctcattc acgatgtcgc ttatgattat tatgggagga gaatggccac
120ctgttcaagt gatcaatttg tgaaggtttg ggatcaaaca tctgatggaa
agtgggtgtt 180aacatcaagt tggaaagcac acagtggatc tgtatggaaa
gtaacttggg cccatcctga 240atttgggcaa gttttagcca cttgctcctt
tgacagaaca gcagctgttt gggaagaaat 300agttggagaa ttaccaggac
caggcgaaag aggtaccaga cattgggtaa ggagaacaaa 360tctagtggat
tcccgcactt cagtaacaga tgtcaagttt ggaccaaaat ctcaaggact
420ccagttagct acttgttcag cagaaggaat tataagaatt tatgaagctc
cagacgttat 480gaatttgagt cagtggacat tacagcacga agtccaatgc
aagctgccat gcagttgttt 540atcatggaat cctagtttgt ctaaaatgca
cccccaaatg atagcagtgg gaagcgacga 600tccaaatcca gcaaatggcg
gcaaagtgtt catctatgaa tacaatgaga acagtagacg 660atggacaaaa
ttagaaacta taagttcagt agtggatcct gtacatgaca ttgccttctc
720gccaaatttg ggaagaagct atcacattct tgctgtcgct accaaagatg
ttaggattat 780taatttgaca cctgtaaacg atgaacagag cattcagagt
ggagtaacta aattggatgt 840agaaaccatt gcacagtttg aagatcacaa
cagtttagta tggagagtat gctggaatct 900aactggtaca gttctgtgtt
caactggaga tgatgggtgt gtcagaatgt tcaaaatgat 960ttacattaac
tcatggaaac ctgtagccgt attgaaaggg gactcctctc aatcctcgta
1020cgatcaaaga accaccagct caacgaacaa cggaagtacc atcataccat
tttaccaaac 1080tgctagatat atcaagttag gatctatagc acagacgagg
gaggcaccgt ggcactgaat 1140ttattataac ttatttatat tgttaactga
tgattttaat ataataaaca ttttaagnnn 1200taaaaaaaaa aaaaaaaaa
1219121680DNADiabrotica virgiferamisc_feature(21)..(21)n is a, c,
g, or tmisc_feature(506)..(506)n is a, c, g, or
tmisc_feature(585)..(585)n is a, c, g, or t 121tcaaaaggtg
ctaatgtcat nttcaaatga gtgaaaagag cgtgggaacc atgcaggggg 60tttcgggcga
acgccggttg cgccaactgg aaatgttgtt tcttggcggg cctgtgctag
120ccaaagaaca aagttttagt atcgaaaccc tcatagatat attacttgtt
ctctacgatg 180aatgttgcaa ttcgagttta aggaaagaaa aaacagtttc
agattttata gaacaagtta 240agcctgtagc cagcacagta aaatctttaa
gattgactcg tgaagatttc gagataatca 300aggtgatagg tagaggagct
tttggcgaag tgtgcgtagt caaatttaag ggcaccgaaa 360aggttttcgc
catgaaaatt ctcaataaat gggaaatgct caaaagggca gaaacggcgt
420gttttaaaga agaacgcgat gttttagtct atggtgatag aagatggata
acgcatcttc 480attatgcgtt tcaggatgta tctaanttat atttagtgat
ggattatcac tgtggtggtg 540atttgttaac tttattaagc aaattcgaag
atcgtcttcc agaanaaatg gctcggttct 600atatagttga aatggtctta
gccattgatt caatacataa tcttaaatat gtacacagag 660atatcaaacc
tgacaatgtt 6801225553DNADiabrotica virgifera 122tgaagagtgc
acagtggtgt atggagaaaa ctgctgtggg tacgcggatt gaatgacttt 60tatgtccctt
ttctgtaaat gagtgttttt tttgtataaa gtaacctaag ggaactaacc
120gttgttgttg actaaatcgt atttgtgata taactcgacc acatgaggcg
tatcctcctc 180aagcatcagt gattaagttt tacctatcga attattggtg
gatcaagacc cggagaacca 240tttgtagcgg ttggctatta ttttgggtta
atcattatca cgatctcata atatgacgga 300tacgagtcag gcggagcaac
cgccccaaac gaacggcatt tccgacttgg aagacgatga 360cagggccaaa
atgagaccag tagacataga cgccgatgtg agagaaatgg aacgaaggaa
420acgagtagag atgataatga acagtaaact attcaaagaa gaactggaaa
ggatcttaga 480ctcacagctg aaagatggat caggaccaag cgggcttctc
caacaaattt cggacatggt 540aggcgtcaac agacaaggcg gaaacgtttt
taagagttcg agctgtgtga ttcccgttaa 600cgacattaga ggaatcgagt
cgatgggata tggcaaggga gaaaagattc ttagatgtaa 660attggcctgc
ctctacaggt tgatcgactt gtacggctgg gcctacggct caagtacact
720cataactgcc aggttgaacc aggatgacga acaattcttg gtcaaccctt
acggaatgct 780cttccacgaa gtgacagctt ccagtctgat taaagtagac
atgcagggcg cggtcctcga 840acaaggcaca accaacttct ccgtcaacat
taccgcatat tcactccacg cagcagtaca 900ttccgctaga ccagacatca
aatgcatcat ccacgttcac actccttccg tagtagctgt 960atcttcgtta
aaacaaggac tgatgccttt gagccaggag tctgttgtca taggtgaagt
1020aagtacacac atgtacttag gcggtcatct agatccagaa gaaaaggaca
aaatcgcaag 1080gaacctagga ccgaacaata aagtcctcct actgaccaac
caaggagcat tatgttgtgg 1140aacgaccatt gaagaagcct tcttcaatgc
cagaaatacc gttttagcct ctgaatcaca 1200gctcaaactt cttcctgtag
gcattgataa tttagttttg ataaatgatg aagtaaggaa 1260aaaaatatac
gacgcagcgc acaaagcacc agaaagtact ccccgaccgg accaaccctc
1320tattttggag ggcaaagtag aaagaaagtg gcgtgtaggc ggtgtagaat
tcgaagcttt 1380gatgaggatg ttggacaacg caggattcag gacaggctac
atctacaggc atccattagt 1440taagggcgaa ccaccgaaac caaagaacga
cgttgaggta cccccagcag tttcctccct 1500tggatatttg ttggaagagg
aggagttata tagacaagga ctctggagaa aacttgaagg 1560tggtaggaaa
ggcaacgatc gcagcagatg gttgaattcg cccaacacct atcaaaaagt
1620ggaaattttg gaaaccggaa cacccgaccc caagaaaatc acaaagtggg
tagccgaagg 1680atcacctagc cattcgagca gcacaccagt taaaattgaa
tcggctcttc agttcgtacc 1740aacaggtact aatccaaagg aattcaaaca
aaaacaacaa cagatcaaag attacagacg 1800agcagacaaa atttctgccg
gtcctcaatc ccacattttg gaaggtgtaa cgtgggagga 1860agctaaaaaa
atgcaggacg ctacagtaac tcaaacaggc gaccatgtag tgctaatggg
1920agctgcttca aagggcatta tccagagagg gcaccaacac aacgccatgg
tatacaaatc 1980gccttacgcc aagaatcctt tcgatagcat cacagacgaa
gaactcaacg aatacaagaa 2040agtcgtagaa aagaaagcta agggtgaata
tgacactgac tacagtgaat cagaaggtct 2100ttcgtcagca gctttgaaca
aaagaattga agatatgcaa attaggtctc caacttctgt 2160tactagtgag
acagaggaag aaagtagaga cgaacctcaa atcttacgga tagaaacgaa
2220gaccgttccc aaacctagtc aaccagaagt tgtattaagc gacggacgaa
ctcgaagaga 2280actatataga actaacaact atgcaggttt tcccaaagga
tcatttttgg agcctaaccc 2340cttgcatttc cacttagtca gagaaagcca
gatcgctgtt agacagcgcc ctctaaagat 2400atctttgact actaataata
gatcttcaga ttcccgttct gttgtcgtta accttgataa 2460ttctaggtgt
atatctgtaa acatgccttc cagtacattc gagggcttta ttgttccgtt
2520tgcatcagca ttgaactcaa gaaatcttcc tatgatcaag gaacaagctt
cacaaaccag 2580ctatgttaac gtagtgaatt atactccttt atatattagt
caatatagaa catgtctgac 2640agaacccgac aatacaaatt cattcgagag
aaatattttg atgaacatgc acgagcagat 2700gctggaagat ttgaacgata
gctcagattc tgagtatgag tctttaaata acaccaaaca 2760gcaatactgt
gaatctacca gaagggatat tgttgagttt gatgaaaatg atcagtgcga
2820caatgagagt ttggttgaag taacagcaga ggtacaggaa atgatgttaa
atatagaaag 2880cagcactgat ttaaatgaag atggtccacg tgagaaccaa
cgttcggttg cagtagctgc 2940agaattatca gaaacgactc cacctacaga
aagtttttct tctgtttatg ttgaatatga 3000cgaaaatgat caatttgaga
atgaaagttc tgttggggga accacagagt catcagaaat 3060tctgactaat
gtagaaagtt gtactaattc ggaaactgta ggaatatttg ttgatgtagt
3120caagcaagca tttgtaatga cagaagactt cttaagtgga gccaaagata
ttttaatgaa 3180aattcctaat gacagatata ttgtcaaaga aaactctgat
gaaggaattg tggattgtac 3240ttttacgaac aaaaccttca gtatcgacga
tgataatgaa aatacaactg tattggaaaa 3300aactgagtat gagtatgttc
aagatttgac acaactgata gtggactatt taatagagca 3360gtgtatggag
gttaaagagc ttattctatt cccagaggtt ctacatgatt ctaacttgca
3420taaattgctt gaaaagatga aattaatata ttatgacgcc tttaaggacc
agttatgtga 3480agataaaaat gagctaagac tttctttagc ttcatcagtt
ctaaggagac tggaactcga 3540agaaaagtta aacaattctg gagattctgt
aacctcgaac acagtttccg ataaaatctt 3600tacgatttca gagtttttga
acgatatttt ggaccatttc tttgacaaca tcgatttaag 3660gcagtttaac
aacttcagtc aggagttaat tgcgaacagt gatcttgaag tacattctac
3720accaaaactg aaaagaagag atataaatgg caacgttgag ccattcgaag
tgaatacctt 3780tcatcaacag accttgtcaa agtcgggctc tgaaatattt
tggatttctg tgtctcctgg 3840tacttctgct aacaatgaac aacctcgtag
aaaaattatt aatgttgatg atattccttt 3900gagacctcct gttgacctga
ttagtaaaga agtgttggac cctatatctc aacctcctgt 3960tgacttgatt
agtaacaaaa tgttggctgc tatacctgaa gaagtttgtt tagatttatt
4020taacacttcg tcaattgaag aaggatttaa accagttgat gataattcca
ctttagtaaa 4080aggacttcag acgcaaaaag gagataatac ttgtgtcaac
gtagagaaaa aaaaagttac 4140taaaattttg actaaccaag tgagttttta
taggagccaa agtgtaaata gtactgatta 4200tgttagtgta aaaagagcag
tatggaacaa caatactgaa aataaggaaa atgacccctt 4260gaacgattct
ggagattgga tgggttacga cactgcaatg ttttgaattg aatttcattt
4320tgtatatcct ttaccaagtt ttatttatat ttgatatatt tctcgttttt
aaatacattt 4380ttatatcaaa gtgtatattt ttcttcgtat gggggaagtt
tttaggggag attaaggttt 4440gaacacaatg tatagtaatg cacaataaat
aaatccacta acattgaata tagacatgta 4500tacaacacat tttttaatca
aaatcactaa tcagtatcat caagaaaagt ttcgacctaa 4560tttcatcatc
aggaatcatc tacctcatgt aagaaaatct aaaattatag ttaatttata
4620tcctccctct aaagttatgt cttcgtgtcg attttgataa caatttataa
atataaagtg 4680aaataattta tcacatttat ttacaaaaat tatatcaaca
cactgtaact ttttcgacag 4740aaacatttta ttgagtaata tttggtcctc
tcactccttc atgccatctc tcttgaggtg 4800gtaaacagta cggaaacggc
tgttctcttt ttcatatata ccacgttttc cttcaatttt 4860tacacagtta
aactaaactg tggttttatt tttcacaagg cgattacaaa catttggccc
4920ttctctgttc tttcatttat aagttgaatt ttttctattt ttcttttaag
ttggactttt 4980ctttttacat attcctgttt gtttacgttt tttatcaaaa
tgtccccatc ttttgcgtct 5040gatagtatac caaagtacta agaataaatt
attgtaatca atttttgaaa acacactgat 5100atgaaggata aactgttttt
tctgttttgt gcattttttg tttgtactgt atacaatttc 5160ggatacaaaa
tcatttcttt tatcggtgtt taaatctatt taatgtttta ctgtaagctc
5220aatctcctgc ttggttttat ttagtttata gttttttctt ttcaaattaa
aataaataga 5280taaaaatcag ctgagcctta accactttct tgcattttcc
taatttattt gtagtttagt 5340agtctagaat aattgtttga ttaatcttta
aaaattgatc agttagtaaa ttttgtgttc 5400acgccttaag gtgtatatta
ataatgtata taatgtaaaa gttattgacc ttataacttc 5460tcttttattg
tatttaacga ggaaactgta ttttaaacaa tgtttactat gtaaaatctt
5520attcactaat aaaatatttt tcgtcaatta aaa 5553123978DNADiabrotica
virgifera 123cggaatcatg ttatcaatgg aaaataaact aaagaaatac gaaaaaatta
aatttttagg 60agaagggcaa tttgcaacag tatatcaagc tcgagatgta gacacggaca
atatagtggc 120tgtgaaaaaa attaaaatag gtagtcgaca agaggcccaa
gatggaatca atagaacggc 180tcttagagaa attaaactac tccaagaact
gcatcatacg aatataatcg gtcttctaga 240tgtttttgga catatgtcca
atgtgtcatt agttttcgat tttatggata cagatcttga 300ggttattatt
aaagacaata ctattattct cactactgcg aatattaagt cttacatact
360tcaaaccctt catgggttgg aatatcttca tttgaactgg gttctacata
gggatttgaa 420accaaataat ttgttagtta attcaagtgg catcttaaaa
gtaggtgatt ttggtttagc 480taaattgtat ggatctccca ataggataaa
tactcatcaa gtcgtcacaa ggtggtacag 540agcaccggag cttctttttg
gagccaaaca gtacagtacc tgtatagata tgtgggctgt 600aggttgtatt
ttagcagaat tgctgctcag ggtacctcta tttcaaggag aatcagatct
660ggatcaattg acaaagattt ttgatgtatt tggaaatccc acagaagaaa
actggcctgg 720gctaaagaca ctatcagact tcatcgagtt caaaccattc
aacgccattc cactgaagtt 780gatcttcacg gcagctggtg acgatttgtt
agaactaata cagggtttat tagtgttaaa 840tccaatcaaa agaaaaacat
gttcagaatg tttgcagatg cctttcttta gcaataagcc 900cgctcccact
attggtacca aactaccact tccacagagc ttaaggaata acagagataa
960tgataaacct actgcctt 9781241132DNADiabrotica virgifera
124cctttcatgc tctgttatac tcaagaggat gctaacgcag aaggcaacga
ggaaaacgtt 60ctatcgtacg aaccagtcca acagttgcat attaattata cgagacctgt
tattgtactg 120ggtcctctaa aggatcgtat caatgatgac ttgatatctg
agtttcctga caagttcgga 180agttgtgtac cacataccac aagacctaaa
agggaatatg aagtcgacgg tagagactac 240cacttcgtcg catccaggga
acaaatggaa agagatattc aaaatcatct attcattgaa 300gcgggacagt
acaatgacaa tttgtatgga acttctgtag catctgtacg tgaagtcgct
360gacaagggta aacattgtat cctggatgtt agcggtaatg caataaaaag
gctgcaagtg 420gcgcagttgt atcccatcgc tgtttttgtc aagccaaaat
cggtcgaatc cataatggaa 480atgaataaaa gaatgacaga agagcaagcc
aagaagactt ttgaaagagc aatcaagatg 540gaacaagaat tcggcgaata
cttcacagct gttgtgcaag gcgacactcc cgaagacatc 600tataaccaag
tcaaagaagt aattaaggaa caatcaggac caaacatatg ggtaccggcc
660aaggagaaac tgtgaccttt ggccccaccc atatggactc ttccgtctcg
tccacaacgg 720ctgcactgaa gacgccacta tttaaactaa tataagatcc
ccaaaatccc cctgtagcga 780atgagcgcta aaagttttca aaattgcgca
ctgatttttg atcaaaaatt gtcctcagga 840gtatttgttt attcatgaag
tattcttttg aaatttgttc tgtctactta tagtttattt 900cactttgttt
ttgattttgt tttattattt tagattcata tcggtaattc ttagtttggg
960taaattagtt tccagaaatc tctaagagat tgaaagacat tcatttttac
atatttctgg 1020gaaaatttgt ctattataat ctttctcttt agaaagtgcg
tattttgaaa tttacagttg 1080gtcgaatcga tcggttattc tcttcgtgtc
cgctacgtat ttacgatctc cc 11321251151DNADiabrotica virgifera
125aagaggagga agaagttgaa attaagattg aagaacatga tgtgaaacct
agcgtcagca 60gactttacca tgctgccgag agcgatcata gctaccacaa gggcaagcag
agtgccatgc 120agatggctac ctttggaatc gagactcctt ctgattcaga
ggaggaaata gatgttgtca 180atgtcaacaa cgagaagttc ccgttcaata
aaacggcggt gttttcgttc cccaataatc 240catcgacgaa agatcgccaa
cagatccaga tgcgtatggc aactgctata tccaagaagc 300gaatacaagc
ccaagctcaa ggcatcaaga ctatcatgcc cgttaggaaa acagccacac
360cggaggcgtc acctatcaaa aggaaacccg tggaaaccac aaggagtggg
aaaaaaactc 420ggcaatacag aacaccggtg agcactccat acaaacgtag
acaatacggc aacagcagcg 480atagcgaacc agaaccatcc gagaagagaa
gtttacacaa caacatggag aggcagcgaa 540gaatagactt gcgcaatgcc
tttgaagatc tgcgagtatt ggttcccgaa gttagcagaa 600agagagagcc
gccaaagtgg tgatcctcag ggaggcagct caatattgcg atttcctcac
660caacacctcc acaaaatatt cgaaacactt cgatgaatta aaaaagaagc
aggaattcct 720taggcggaga gtgtcacaac ttcgaaggaa tttagcagca
aatcgttaag taaatataga 780atttgtaaga aataatgcga tttgtagtaa
agggtgtgtt tagaattgta tagaaaaatt 840ccgagtatcg gagagactaa
tgagtgactc aaaagagcga aacatctcag ttatttgtta 900tatttttggt
ttcaaaaaac agaacttgtt ctagcaacat tatatacatt atcttaaatt
960ttcagtctcg aaagcactaa taggcaaaag caggttgttt tatcctgcga
tcattgtgga 1020tagctgaagt gatcctgagt tcaaaaatac aacaaacaaa
aagatgttac gtttaccagt 1080cgtattttat accttacatt aattgtaatt
gatttttgaa tgccgttttt gttttttcga 1140aatttggtca c
11511261529DNADiabrotica virgifera 126ccgcctgatc ccgcagatct
ttccaaggga atggaacaaa ctcctattcc ggttgtgaat 60ggaatcaata gagaaatgtt
agacttttgt aattattcaa ttaaacgagt tcctatggaa 120ggcgttgata
tgaatctcga tccagactat ctgtgcggct gtgattgcga tgacgattgc
180attgataaga cgaagtgcgc ttgctggaag ctcactttag agggagcaaa
gtataccgga 240aaagatgtaa atcctacatc ggtcggatat atttatcgaa
ggttgcccga acaagttatt 300acaggaatat atgaatgtaa ctccaggtgc
aaatgttcat cgacgtgtct aaacagagtg 360gtgcagaacc ctatgagttt
gaaacttcaa gtatttagaa ctcacaatcg cggttggggt 420atcaggtgtc
tcaatgacgt tcctccaggc agctttatct gtatttatgc tggtacaata
480cacactgaag ctatggcgaa tgtggatggt gtggcgttcg gagatgaata
ctttgcagaa 540ttggattata tcgaaaccgt tgaaaaatat aaagaagatt
acgaagcgga agtcatggag 600gaggaggaag aacaaaataa gaataaacgt
cgaaaggaac ccgaagagga agaggaagaa 660gaagaggaat ccgtagcggt
gatctctaac agcaaataca aaaggtacaa ttatgctgca 720gataacgatt
ttacacctag tcatcccgac aggattgttg cgggagatgc accaattaga
780acgcgtctaa gaaagagaaa caaagatgac gctaaagatg aagagaacga
agtgaaagga 840caaaaaagaa gactcaagga atgaaatgat agaaaataaa
acaagaaata tacaaataaa 900aataattaca gagtctaatc aatcaatgaa
ggcattaatc gaaggactgt ctgataatat 960cgaaactgtt accataagcg
acgaagaaga tgagggaaga gaagtattaa gttttaatcc 1020acaagcgagt
aacttggacg acaaagcacc caattacacc tctgtccgag atctctacgg
1080aaaagacgaa tctgtttatg tgatggatgc aaaaaacgct ggaaacattg
gaagattttt 1140gaatcattcc tgttcgccaa acgtgttcgt ccagaacgtt
ttcgttgata ctcatgatcc 1200ccgtttcccg tgggtatcat tcttttcgtt
gcaccatatc agagcaggaa cagaactgac 1260gtggaattac aattatgaca
ttggcagcgt tcccggtaaa atgttgacct gtcattgtgc 1320ctcaggagaa
tgtaaagggc gtttattgta atctgacaga atttgtactg ttctgcaggc
1380aatggagatg tattatttca tacattacgt aattatttat accgtttata
atagtgtgct 1440gtattttatt aatttcgtgt atcaaaattc aatatttcgt
tcattgattt tgtctttcat 1500tgtgctaagt attagaataa tatataatt
15291272613DNADiabrotica virgifera 127aaacctactg tgtaggttcc
tttgtttttg ttaatgagaa caagtggtgt tgtattgagt 60gaacaagtga aaaatattga
gtataattaa ttattcacat acaactggtt tttgttttca 120caactggaat
acatatctta ttctttaagt taataatcaa gttcaacaat gtggcaaacg
180caggcagtcc atattaccac aaacaataag gagaaccact ttcaaaaaag
tgaagataag 240ttaactcttg gaatgacttc tgctatatca acagcagaac
caaaaccagt
tgatttggta 300aaaacaaggg aattaataga ggctctgaaa ccttttgatg
tattcgaaag tgaacaagaa 360ttaaacaaga ggatgcacat tttagggaaa
ctgtttagtc tagttaaaca atggataaag 420gaggtttcac tttcaaaaaa
tatgccagag agtgttgctg agcaagttgg cggtaaagtt 480tatacattcg
gaagttacag attaggggtt cataataagg gggctgatat tgatgcactc
540tgtgtagctc caaggcatat tcataggtcc gatttctttt cctcctttta
tgaccttctt 600aagcaacagc cagaagttac tgatctcagg gcagtagaag
aagcatatgt gcctgttata 660aaaatggact tcgatggtat agaaattgat
atgctctttg cacagttatt acttaaagaa 720attccagaat caatggattt
gagagatgat atgcttctta aacatttaga acctaaatgc 780gtgcggagtc
tgaatggttg tcgagtgaca gacgagattt taagactggt accaaatatt
840gaaaacttca gaatggctct tcgagctatt aagctttggg caaaacgaca
tggtatatac 900agtaacgtat tgggttactt gggtggtgta tcctgggcga
tgctagttgc aagaacatgc 960cagttgtatc caaacgcaac atcatctaca
cttgttaata aattcttcct tatattttcg 1020aaatggcaat ggcctcaacc
agttctacta aaacaaccat ctaacgtcaa cttaggattt 1080gtggtgtggg
atccgagggt aaatgttcaa gatagatacc atctcatgcc aatcattacg
1140ccagcttatc cacaacagaa ttccacattc aacgtttcac aatcgaccag
aacgattatg 1200gttgaagaat tcaaacaagg tctgcttatt accgacgaca
taatagtagg caagtacgga 1260tgggataagc tgttcgaaca accgccattt
ttcactaaat ataaacattt cattttactt 1320ctggttatgg cagataattc
cgatgatcat ttggaatggt gtggacttgt ggagagtaag 1380gcgaggttac
tagtaggtca gctggaaaga aaccaataca taaccctagc acacataaat
1440cccgagagct atgccatgct tgaatccgcc atggaacccc atacattatg
ttccatgtgg 1500ttcataggac ttgagtttgc caaaactgaa ggtatcagcg
tcgatctaac aagagacatt 1560cagacattta cggaacaagt caataaccat
gctatcaata tcaagatgct gaaggatgga 1620atgaggttag acgccaggca
tgtcaaaagg aaacagctgt cacaatatct tgctccggga 1680gtgacttaaa
agagaaagga aattgagtat cagtaagaac ggggttacac cggaacaaaa
1740tagaaagagg cagtcttcgg aagctactca agaacacaac gatgctccaa
gtaaaaagac 1800gaggttgtca gaggatatcg cgtcacaaga gttcgatgaa
tgctccaaca cgtcaataca 1860catagattcc agcagtaatt taagtctaga
atcagagagt aacattagtt gtacatcccc 1920ggcctcaact ccctcccata
taaatacaaa ttccaccacc acaaacgtac cagaaacggt 1980gtgtacgtga
caacctacaa ctgatgtata tgtgagtcgt ccattcgttg ctgtcaaata
2040aaataatttt tattgtaggt ttcttccaag aaacaacagt ttatgtggtg
ttcacgtatg 2100tattaaatta cttttccaaa aacagacaga aaaacttttt
tgaggtaaaa tattatcgca 2160aagagtgcaa tgttcttatt ttaaggctta
atgtgtttat tgtgagtgtg cgttaaaaat 2220tatcctgtat atacaccaaa
aggattcctc cgtcgtcaag gaaagataat ttgtaggcag 2280atagaccgac
tgcacatgaa agcggatgat ctaaaatcca acgaaaatac acaggatgta
2340tcacttgtaa aatgttcgtt tatttgttta tcagtttaag gattctttaa
tggtttagat 2400ggatctagcg atttagttaa cctatggagt aatttataat
tgaaaacata agtttatgag 2460gagtttaaag gtattgtctc attttcttct
atttttagtt gtataccatg gagtctgata 2520ttttttcggt ttctcgtcca
ataactaaat tgtcgttcta tatttccctt tagtttcgtc 2580aacactcata
acagtctgcc ctcttgtgtt taa 2613128450DNADiabrotica virgifera
128atgaggatag atcaactatt taatatcatt atagaatacc cagaatctct
accggcttta 60gaagacctac gaatatgtct tcctagaaca gatctaaagc ctatgctaac
taagaaacta 120caaaaagcaa tggaaacacg attactacac cctggtgtaa
gtacacctga tgttctaaca 180gcttacgttg ctgccattag gtctttaagg
gtgcttgatc ctacaggact attgctcgaa 240actgtcacgc aaccggttca
tgaatacttg aggagtagag aagatacagt aaggtgtgta 300gtcaccagtt
taactgagga tggtcctaat gatctagcag aggagttggt gagaggagag
360gccgttcaag ttgatgaaaa tacaccactg gacgaagata atgaagactg
ggaaacttgg 420gtgccagatc ccatcgatac agtaccaaat
4501291812DNADiabrotica virgifera 129ttgatcagaa gagacaatat
atcacatgaa tagaataaga agaaagtgaa tgatatgaat 60acgtccctga cgtttataaa
atttgacatt ttacaggaat tttgcacgaa tttactagaa 120acatggtttt
aaataacgtt aaagaactaa ccgaagaaga aaaattcgat attcagctgc
180aagctgagca ggaactgggc agactagtca gaacttatac gatcctagaa
agaaaccgag 240cccaaaaatg tgatacaggt ggtaaacttc tcaaacaacg
aaaagtgctg gacattttca 300agaaggaaca aaaaaatatt ctcacggatc
ttgctgtagc gtctgccgat gctagaagag 360tggaggacga aaagaagtcc
aaggaattga aggatttact tagagagcac gacgatttcg 420acgctgaaat
cactcaacaa aaatcacata ttagtgagat cgatggacag attcgaatga
480ccaagaaaaa ggttcttgac ttaaggtcga agcaaattac agacgaaatg
tatcaacaaa 540gagtaattgc aggagaaaag acggttcaaa ctttagaaaa
caaactggaa gtccaaataa 600agaaattttg ctcaatatca gccgagaata
tcaagttaag agaagaaatt gatcatttac 660ttttagaaag aaacgatttt
aacaaaattt gggacagcct tatcaacaat ttagctgtcg 720gaaagaagtt
catgttggac ttaatagaac aagcaactat agcttatgat caaagggaag
780agtgggtgtc caagctacag attttaagaa caaaagccca caatgatctg
attaatcaca 840ttcaggaaat gagggtgttg caaagaaaaa tggacgacga
tcagaaacta caagagtttt 900tctcagtgaa atgccaaaaa agaatcatga
gacaactaga agaaaaagaa aaaaacaaaa 960ggatgctcaa taaagaaaat
atggaaaaga aattggaacg ttacttacaa attttaatta 1020cgattaagga
atttaccgga gaagaaaaag ttacgacgat tgccaacaat tttgtaaccc
1080aagaagaaga aaattttgcg atgtttaaat atattaatca tttgaacaaa
gaaatggaag 1140atcttacgga cagtctagca aaattgcaac tcaaaattgg
cgagcaggag gcactaaatg 1200aattgcgtaa gcaccaacaa gaaactaggc
tggaaaagct gaataaagat tttgaagatg 1260ctaaacagat cacgagtcaa
aaatctgaag agctgaaagt aattgatcaa aagttgagaa 1320cgattataaa
tggcatcggc caattgttta gaatgtttag atgcaaaaat gatcctctaa
1380taaagttatt aggtgaaaat caaaacatac attattataa tgtactgctg
tatctggaaa 1440ttttggaaaa caacatcgaa gaggcaatgg tttctgtcaa
tttcaaggag acccaaactt 1500tggaaagaac aaaacacaag gaacccaaaa
taacaacttt gagacacgtg aaatgtccac 1560ccatgatcga acccatcgag
agaatagtcg ctactaatcc ttgccctttg tgcgttgaac 1620atgagcatgt
cagtgatgtc atagatgtac tgcaattcgc gcttaccaaa gaagagatcc
1680aggagaggct taagatcaaa atcgataaaa cagaggcaga cgacaagttg
cataatgtat 1740ccgcctgtca tctgccgaaa tccagacaaa ttatacaaaa
aagatatcaa taaaaattat 1800aaagtgatca ct 1812130830DNADiabrotica
virgifera 130tttttttcat ttttgacacc gactttgtca tttatttaac aattatatac
taattttaat 60attacgattt taattgatat aattatatct aaaattcaat aatggctaga
tacgatttga 120cattgaatcc catggaagaa accaggtttc ttaaaaaaca
tagagtactg gctttaagtt 180tggcaagaca ggtccatttt agctttcagg
aggttgaagc cattctcatt atatattaca 240agatacaaaa atacgacact
tacgatccaa aaggtattac gagagacgtt ctgatggaag 300ttttccattg
ctgtttagat atgacggata tggaaaaagt gattcgaata ataacttgcc
360tagacgagaa agctcgagat gtctacatat caaaagagct ttggtgcaac
atgttatcgt 420tatttttaag aggaacgatt gaagaaaaaa tggcattttg
tttcaaagtc tatgattaca 480gtggaagcgg acatctcatg aaggaaaatc
tttttaagct tttagtggga tctttgaaga 540gcaacatcgg cgaatcagat
gcggaagaag ccgtgaagga tatggtggaa gttataatga 600agaaaatgga
tgcagaccgg gacggcaaga tttcctttaa tgactaccga tataacgttc
660tccgccaacc agacctgttg gagtttttgg gccagtgtct tccagacaga
catacggttc 720acaaattttt gtgtaccttt actgaaaata ctaagatccg
attctataag ttaaaattag 780gcacaagtat aatgtgaaat taggaagtaa
acgattaaaa gttaaaaaaa 8301312072DNADiabrotica virgifera
131gttaaatatg atacatcaca ccaattcgag gattataaca actacaggtt
aactttacgt 60taaagataac ttcggtcatc catcacacgg ctgattagct atctattgac
aggttgtgta 120tatagagcag ttccttgtaa cacgacttgt ctcttctatt
ataatgtttg tctcactatt 180ccaatcagtt ttgaagagtt tttaagccca
cacttcataa catttggtag atgaattcct 240caatttaact atgaggataa
aagtacgcat aaagtcacaa aaaatttccc cgcttttccg 300tgacatcctt
attccgcaag ttatattgtt tctgcgcttc ctatagacat gggatttgga
360ctaggagtcg agctcatcga gctcaaagta tcttctttca acctcaactt
attcggatcc 420ttctcctttt tttctttatc cttatgttta tgttcgtgtt
tgtgtttatt aacgctaaaa 480gacgtactat tttaccgaaa tgatgttatt
actacaataa gttaatgatt tttgaacaaa 540aaataagttt gtttggtgtg
ccttctacat aatttttaat tataagtttc atatagaaat 600aaatttttaa
tcatggccgc gccaaggaga gccgcacaga ccgtaaatca agaaaacgta
660aattcccgtc ttgctgtgaa atcaaacgta aatgcgccat tagaaccgct
aaaaagacca 720gcacttggcg aactcggcaa tacactacaa gacacccaaa
aagcacaaaa cggtcttgct 780ccaacaaaag atggttttaa aaaacctgaa
ggtactttgc aacccaaaaa agagacaatc 840aaaactttga ctaacaagga
aaaggcaatt ccgatcaaac ctagaactat cctaaaaaga 900caggaaagtt
tagtaaaacc gttacaagga aaatgtacac agcctaaaga agtaccacgc
960acatcgacac tagtaaacaa aaaactgtct atcgttgacc cagacgaaag
tagcaaaaat 1020gatcctcaga tggtaaccga gtatgtaatg gatatcttca
aatatcttag agacatggaa 1080ttgaaattag ctatcaaaga taacttcctc
aaagaccacg aaactacttc caggatgcgt 1140gcaatactcg ttaattggtt
agtcgacgta cacgcaaact ttaaagccac gcttgatact 1200ttgcatatat
gcattggaat cgtggataga tatttacagg cgaacaaaaa agtaggaaga
1260aatacgcttc agttagtagg ggcctcagca atgttgatag catgtaagta
tgaagaaata 1320tatgttccag aattagacga ctttgaatat gtatgtgatc
acacatttac aaaacgacaa 1380attcttcaaa tggaaaggga aatactgaag
gggttagatt ttagtttagg tcgaccgatt 1440tcggtgcagt ttctccgtag
atatacaaaa gtgactcaat ctcgcgtgga gcaccataac 1500ttaggtaaat
atatgttgga attggtatta ctcgaaagcg atctagtgca cgtaagacct
1560tccctactgg cagccgcagc ttgttgcttg tcaataggaa tattgaacga
aactatggat 1620ttgccgaaac tgtggaaccc cacctgtgta cagtacacgt
cctacaatta caacgacttc 1680aaatcgataa taacggaact cgcttatctc
ttagtcaaaa gcgaacagtc gaaattccaa 1740gcaataagaa gaaaatacgc
cctagcaaaa ttcggcaaaa ttagtcttaa tgttaaatta 1800aatggtcctc
ttgtgagaaa gttgacagcg gggaaaaagt gatatttcca ttttatcttt
1860taaccgaatt tttatatttt atatcagttt tatatttagt gtcattttta
tttacgtttt 1920agtacatttt aataaattag attcaagcta aagactatta
aaagccttca atatcaattt 1980ttgttagtca gtttttattt gacggaatgt
gaagcaacaa tggagcaata aagttaagta 2040gtccaggctg tttgctcgcc
cccgtcaggt aa 20721321517DNADiabrotica virgifera 132gacagttaat
acgcgaaaaa gaactaatac accgatgaaa ctatcgtatt aaaataaagt 60ttaaagtttt
taaattatgt gtggattagt ttagtgttta gtgttatgtt gtgttagtgt
120tttttttctg tgactaagcc tacaataatt tcttcgagca acaggaaaaa
atttaaaacg 180gagaggttct gaacatttgt atctgaaata aattaataaa
gatgaatcaa acgtgtaaag 240tgtgtggaga accagcagct ggtttccatt
ttggtgcttt tacctgtgaa ggatgcaagt 300cattcttcgg acgctcctac
aacaacctaa gctcaatatc agaatgcaag aacaacggcg 360aatgtgtcat
caacaagaag aaccgcacag cttgcaaagc atgtcggctt cggaaatgtc
420ttttagtggg tatgtccaag agcgggtccc gatacggtag acggtccaat
tggttcaaga 480ttcattgctt actgcaagaa caacaacagc aacaacaagc
ccatttagcg aatgtttcac 540agaacatgaa accaccacag aagacgccac
cattacatcc gcagccgcca ctaggtatgg 600gccttcttgg aatgaattcc
tttcaaccgc cacttttaca tttacccaaa acaaaggaag 660agttaatttt
attgggcttg gatgaataca aacactcagc ctctcctcaa gtcagttctc
720ccgaatcaca caattcagac tcatcaatag aactaagcga tgctaggagg
ttacctttgt 780ttcctggact attacctcct acattcctgc cacctcctgg
tctccttttc cctccaggat 840acccccctct ataccctggc ttactgcaac
ctgccaataa caacagactt atgagaaacc 900acaatcatgg tgtcgaagca
tttaacaagc gagtgttcct ggatgcagtc cttaaatctc 960agcggtcgcc
aactcccgaa gaaagaataa ctcccatatc cactgaaacc ataatccaag
1020aagatcctat cgatctcagc atgaaaacga acagcgatag ggggtcttct
ccagctcata 1080gcgaccaatc gggctcagga acggagcgag gaaatggtag
cgaagccgat gaagaaagtg 1140attgcgagtc agatagagaa atcaaaagga
tgaaactcca caggcccacg ccgctagatc 1200tcacgacgaa agtatgatgg
aacatatttt tcatattact gatattgctt tcggagtacc 1260tgaataatct
agataaaaga ttttcaggtc actgatcagt ttctgggcca gtcttgaagc
1320tatctggatt tccgtttcga aactgtcgat ataatgcaga tcgaaaatga
aagtgttcaa 1380tcttttactc aagaagtcac ttggcggttt tcttaataat
atttagattt aaaatggaaa 1440aagtaaaaag aaccagtaga atattaagtg
tttacatttc attgctaaac tgattttggt 1500tatttgtgat tgatttt
15171333192DNADiabrotica virgifera 133tacacgtttt gctacttttt
gtattatttt ttccaagtgt attaaggata aacctttcac 60gggattaaaa tttcgtgaca
aacaggccta ctattacaag aaacttcagt gttgtgttta 120cttcactata
caagtttgca actatgggta caaatactaa ggacgacttt tcagaatcaa
180aagaatacgg cgtaaaatct gaggaattcg aagacttttc tgagactatg
ttagatggaa 240gcgataagaa atcattagag gacggctcat tgtcagatcc
tgataactgt ggcatgtgtg 300gtggcccatt ggttatacct cgaatgctgc
actgtttaca tacattttgc gaagaatgcc 360taggcaaaaa gcttgtcggg
gaagctggag atgctggatc cacagacact tcaatccaat 420gcccgatctg
taactattca accctggttg gaacaaagaa gttagctgct ctaccgcttg
480atgtctcaaa gacaaatgtt accgacgttt ccaactcccc cgatttgcat
tgcacttcct 540gcactgccaa agaaatggcc aagtccaaat gtaacacctg
ccaacacctt ctatgcaaca 600attgtgacaa ggctcatcat tttatgagat
gtttcgagtc acacaaggtt gttgctctcg 660aggacatggt taaatctggt
attaagatca cagttcacaa gcctctagta tgcgatttcc 720atcctggcga
aaatctcatc ttctattgct tggtgtgcag tgtaacagct tgcacggagt
780gtattaaagt tgatcataaa ggccaccagt ttgaggggat actcgactct
gaaccaagag 840ttagacaaga gatggagggt ttggttaaga aaggcaaaga
aaaaatcgaa aacctcacca 900aagcttctgc tactttgaac aataacatgg
aagaactttc ccaccagcgc tccaccgcta 960gagatcttat caatgaatca
tatcagagtt acaaggctgt tcttgagaaa tgtaaggatg 1020atattttaga
agagctgact ggattgtatc atgaacgtga gctagtcctc atgcaagaga
1080gtaatgagtt gggcaatacc attgttaact tagaagatgc ctgtaaatgc
acttccagct 1140tattggaagg cggtactgca caagagatga tgtatttaag
aacagcactt gctactaggt 1200tgatgacttt aaaccacaaa tcacccaaac
tggaaaaatc attcagtatt gagttcaaat 1260ctgacttgga taagtttgaa
tatgctatca ggagtaattt cggaaagttt gctacagagg 1320aagatgtcat
cgtgaaaaca tcacagtctt ctccagtttt acctgaattg gctcccttga
1380acatcaacgg gatcaatgga atcaacaact caccacctgc tatcgtcaca
aacggttgta 1440gcgcaccttc ggtaacaaca accagtccaa tttctttacc
cacatccatg caatcttcat 1500tcgacgggga acttggaaac aatttacaaa
tgttgatggc tcagagtcct cccttacccc 1560acgtcaattc aaaccaaggg
ttttcccaag ggttttctag tattgccgag tacaatatag 1620cacaactagc
cactttagca gaaaacacaa acagtgctgc cacgtcacca actacagctc
1680cgttcaatat tgccgatatt ttgaacaatg atactgcctt caagaatatt
gcaacgttag 1740ttaagctggc aggaaccaat actactccgt cgattcctag
atccaacaaa gttagtccta 1800tgcagatccg ctgcaagttt ggccagttgg
gacccggcaa ggggcagttc aattctccac 1860atggattttg cttgggattg
gaagaggaca ttatcgttgc cgatacaaat aatcatagaa 1920ttcagatctt
tgaaaaaacc ggaacattca agtttcaatt tggcatccca ggtaaagacg
1980aaggtcagct gtggtatccc aggaaagtag cagttatgcg caataccggc
aaatacgtcg 2040tatgcgatcg tggcaacgaa cgctccagga tgcagatctt
cactaaaaac ggccatttct 2100tgaaaaagat agctatacgt tacatcgaca
tagtcgctgg cctagcagtc actgtcaatg 2160gtgagattgt cgccgttgac
agcgtcagtc cgacagtatt catcataggg gagagtggag 2220atctgctgcg
ttggttcgat tgcagtgatt atatgagaga gccatccgat atcgctattc
2280atgggaagga attttacgtt tgtgacttca aagggcacaa cgtggtagta
tttaacgacg 2340acgggcaatt tttgcgtaga atcggatgtg agaacctaac
caactttccc aacggaattg 2400atatatcaga tgcaggagac gtattgattg
gagattccca tggaaatcga ttccacgttg 2460cagtcttttc acgcgatggg
ggcttgattt cggaattcga atgtccatat gtaaaggtct 2520ccagatgttg
tggattgaaa atcacatccg aaggttacgt agtaaccctc gccaaaaaca
2580atcatcatgt tctggtattg aatacgttat acatcctgta aaccccgaat
aatatagcgt 2640cgtcgacgaa tattttgacc ttagtccctc ttcccgacca
actgtttcat gaatatgttg 2700tgttagttga catcctctga cacatgttgt
gttagttgac aaccctgaca cattctcacc 2760cgaccctcgc ctgttgagct
gtggcattta acaatttttg ataaaaatat ttttatgtat 2820ttaatcttca
cagttcgtac ccagaaatag actaagaact ccccgtaaaa atatgagtag
2880ataaggtatc ggtacgacac aaatcaaaat gtgcgtagaa gaaagttaac
attgtgttga 2940aacttacgct ggtcacggct tttgttacca gctgctcaaa
taattatgct tatattcgta 3000ttaacgttta cactgttctt taaaaactat
ttaaacattg atataaggtt tgttccaaca 3060gaacgaggaa ccgtcatgta
acgattatgc tcatgcgcag taagcaaaat atcccattgg 3120aacgtattat
tttgtttact gcgcagtaaa gcgtcattgt cacatgacga tttttcgttg
3180tgttggaaca aa 31921341578DNADiabrotica virgifera 134tatttttaaa
cacagatttg aaatgtggga ctcttttact attgatgatt gtcaaacatg 60tccgtataat
agcactacaa atatatatga ccaaatggaa gttccacact tggacaagga
120aaatattcag ccgttcaatc acataaaaaa tgacgagctg tctctatacg
acaatggagg 180cagtatcaca aaataccgcg aagcaccttt gtctcatact
caccgacccc tagaagaaca 240cgactcaaat tcgcaagatt ctggctacag
tgcaagctac catggagaaa aatttttgtc 300ttatgcctct ccagtacgaa
ctgcaagcac atcctttggc tctatggtct ctatggaaga 360tgaatatttt
gatttctctg atgttgaacc tttggagaaa ccaaacttac ctcacgattt
420caataagctc ataacaaatc ctttgattaa cagaactaaa cctgaagtag
tctcatctcc 480aaaagacact atcataagac cacttttcag aagggcttta
tcacttcagc ctatcagtga 540gaacacacct aatagtagaa gggtccgaac
aagtttattt agaggtgaac atgaaatcag 600atcatttaaa agacctgaac
ctccgaatga tctagacagt acaattgtga aaaggtcaaa 660gatatttaac
gataatgatg atagtgattg tgaagcttca gttcctgtag ccaggaccag
720acctatacta caacgcgcat tctctgctac agaagacagt attatgtgcg
cagtgcaaag 780atctgcaatt gaacctgacc ttattggaga ttttacaaag
aacttcagtc ttccgttaac 840aatcagtcga caccaagatt taaaagccat
cacggcagat acactagctt cactgatgag 900gggagagttt agtgatgctg
tggattctta caaagtcatt gactgcaggt atccttacga 960gtttgatggg
ggccacatta acgatgcact taacttctac accaaagagc aatgcatgca
1020actactaaac tcaacacctg atgttgctga aggttcccac gcaagacata
tattggtttt 1080ccattgcgag ttctccagcg aacgtggccc taatctatac
cgatttttgc ggaaagaaga 1140tagaaacaaa aacgaaagtg tatatccttc
tcttaacttt ccagaaatct acctcctgga 1200aggtggatat aagaagttct
ttgagactca tccagagttc tgcactccag ttgcatacaa 1260agaaatgctg
catcctgatc acgaagatga atataggcac tttcgatcca aatcaaaaac
1320gtggaactgt gattcacgcc aacgtctatt gaagaaaagg ttaggtgtgt
aaaacttgtt 1380taggatgcca tataacttta tagtacaaat aattagctta
tctatttggt tcagttcata 1440gtacctgttg tcaaaaacac gagatatatt
attttaatca ttcagacagt tcatgtctga 1500tgaaccaaaa tctccacacc
acttagtggt ctaggtgaag tgccttttac tattatttgt 1560tgcctgatac cagaagaa
15781352465DNADiabrotica virgifera 135cgtttagtag tacatagaga
aatcgctttt agttttaatc ttttctgtgt tggaatattg 60aacaaattct aatgattcgt
aaaataacac ttctgttaaa aaccaaaact gatagtttgt 120tcaaaaaaag
tacaattttt ctgggcttgc tactgaaata atgtgccata ctctaattgt
180ggaacaaatt gtaggtatat gttgaaagtg aacaaataac taatgcttac
aataatgtcg 240acatcaagtc aaaataatgg taaaaagagt tcaagcagct
ctattgttaa caccccttcc 300agtactccca ttaccaatct tgcaaatggt
agtagtagta cccaaggaac agcacatagt 360tcacctccac ctaataacaa
tgctccaaag tatggtaccc tagtgcctaa cagaatattc 420gtgggaggaa
tatcagctaa cacaacagaa ggagaattga tgcaactttt tagcaactat
480ggtactgtta aagctgctaa aattatacag gacagggctg gtgtgtcaaa
aggatatggt 540ttcatcacct ttgagagtga agatgatgct aaaaggcctc
ttagagaagc tgaaaacata 600gtgctaagag aaagaaaact taatatagca
cccgctatta aaaaacagcc ttttagtcga 660gcatttgatg catccagtcc
acctgctgtt gctgcaaata gtgccagtca attctttttt 720cctccgggtg
ctgccgtgcc atattttcag agtggagtta catattatac
tcagccagct 780cctgctgcac ctggagatcc cactgctcaa caacctgttt
atcaaccccc tccaatgtat 840cccacgcaaa ctggtcctcc acaggcagct
acataccctt ctatgatgtt tcctgctcaa 900actatatata tgccgcaaca
gtatcccatg cctattccgt acgagtacaa cttctaccag 960ggtaacggac
cgtcgctgcc aactcagtat ctggccggag gccaaagcgg tccgggaaac
1020tctcattgca cctcattacc atcatcaaac agtccaccca gacccaattg
ttatggtcaa 1080caggtgcctg cctataattc aggagatccc gtgtactaca
acttgccaat atacggagcc 1140actttggaag gtccgccatt atacgctgat
gccttcgatc taagcgctag tggagcttat 1200gcggaggaaa cctacagcgg
catgattact caagaaaatg tagaagcttc tcttggatca 1260tcggtaaatt
caactcgtgc aacttccgat aattttacgc aacatatgga tcaatctctc
1320gttgagtctg attcaaatat tgttaatgtt ggaggtaaac caccagcaca
taaatcttca 1380gagacaattg tttctactcc ttcccagcct caggaggaga
gaaactcaca cacgcctatt 1440gtttcactac tttccataga tcatcaacag
gaaaaagact attcatcaat gcaaagcggt 1500cgtagacgga aacttctgat
tcaaagcaat caaaataacg tacccatgta tcctacaaac 1560ggatacgtca
accagtttgt cggatataat cctcaacaac catccccacc ttcattcggt
1620ccacctctct ataacaacgg ctacggttat tctgaatatc gtcgatttaa
cggataccca 1680gatctcaggt caaataataa tagtaataaa ccgaggagac
gaatatacga aaataggcgg 1740tcgaacgatc attctagccg ttccagtctt
cgcacagact caaactcatc ggctagttgt 1800gttgacgaaa ataaaaacga
agaaaaaact tttcgaacta ctgttaacac tcctccacct 1860gccccatact
cacctatgac caatcatcaa ttcaaattta ttaattacac taacacgaaa
1920aacgtttata atcataagta cgctcacacg tttataatca taagtacgct
aacaacaata 1980attattataa tagaacagct acacacaatg accgatttaa
acataccgaa cctttagcga 2040ataaaaacaa taacagttcc atttacccat
taacaaataa tttcctaaat aatagccata 2100cccaaagtaa ttctgattcc
cttccctccc aaaattccca ggtacatgtg acgactacgg 2160gtcagagctt
cgtcccggcg gctatccaag ctcagacgaa acgcaacaag cgttctttga
2220gaagaagcgg tgccagtgcc ggaggcatca acgaaatcgg cgccggtgac
gcccctttac 2280caggcgaaga atgcgaagat gtctacaaga agctggaaac
gcttaagttg taacgaaaaa 2340gtggtatctt ttcttgtata tttttttcta
tttatttaag actgtacaat aatgtaaaat 2400actagaaatt taatgtaaac
taaagaggaa aaatatataa tacatattta ttgaagatga 2460aaaaa
2465136275DNADiabrotica virgifera 136caaggtggaa ttttaaggtt
ataccgcaca cctggttgta cagcagcaaa aaagtttgaa 60attgtccaga aacttcagca
aacatgtaaa aatctaaagg atattcaaac cgaaatatgt 120tttcatatcg
aatcaacaag tgccttggaa gcaaaagacg ttaagatttt gaaatgggtt
180cttcaagatc ctctccatcc ccaaaattta agcgaaaagg gaacgctggt
agcgaaaaat 240ggtacggctt tggttgaggt aggacctaga tttaa
275137275DNADiabrotica virgifera 137atggtggatt ctttaaagtc
cattttcgac gaaactcctg aagatgaaag tctctcccaa 60aagattttac aatcagtggc
tattgaggtc agttcggcag acaaattatc caccaagatc 120tgcaaagagt
gcgtagaaaa agtaactgac tggtcttcgt acaaagaacg atgtctccaa
180aaccaaaaga aattaacgga gttgttagtt gcgaaagaag gtccagaagt
tttggttgta 240cctgaaatta tacccgaacc cgaagcacca cttcc
275138275DNADiabrotica virgifera 138aatgatcctg aatacgactt
catatctgga ttcgttcatt tttgtggaca ggctggacta 60gttggtatac aaatattgtg
gacaaaagaa tccgaaattg ctattaagaa agcaagagta 120gatagaatgg
ctatgaaaat tacaaataaa cgatttctcg acctcttaaa tgctctcatt
180gatcttacat caaaagattt aacaaaaatg caaagaataa gatttgaaac
catggttact 240atccacgttc atcaacgaga cattttcgac aacat
275139275DNADiabrotica virgifera 139tggctcctcc accatacgca
gacctcggca aaagagcaag agacgtattc ggcaacggtt 60atcactttgg cctaattaaa
ttaaattgca aaaccaaaac agccagtggg gtccaattta 120gtacaggtgg
tagttcagac catgaatctg gaaaagtgtc tggatctttg gagagcaaat
180acaccgtcaa agattatgga ttgactttca cagaaaaatg gaatacaaat
aatactttgg 240ggactgagat cgccatttca gaccaaggta ttaag
275140280DNADiabrotica virgifera 140tgggcaaaaa gaaggtagaa
gagagtgaaa gtgaagagga gtattctgta gagaaaatta 60ttgataggag agttgtagat
ggaaaagtag aatattatct aaaatggaaa ggatattcag 120aagatgataa
cacttgggaa cctgaggaca atctagattg tcctgaactt atttctgagt
180ttgagaggaa tcgtaaaagt aagaaggctg ctggcaaaga aaagaaaaga
acaagagata 240attctacatc ttccgtggat tcaaacaatt catccactaa
280141275DNADiabrotica virgifera 141tgagttttat aattattgct
tttaaaaatt tcctacatac catgttgcag ttattatctt 60ttggtaaaaa aactattaca
aatagtttga gtgtgtatgt aaaaactatt ggtggcaaca 120ctttaactgt
agatctagat cctacatggg acattaaaaa tgttaaggaa atagtggcac
180cacaacttgg attagaacca gatgaagtaa agatcatttt tgctggaaag
gaattgggag 240acgaaattaa aattgaggaa tgtgatttag gtcag
275142275DNADiabrotica virgifera 142tgaagggaga aaatttaccc
caacagaacc cagtgccttc aggaaatgaa aaagatgtca 60gtaacaaaca ggaatcgtct
aaaaatcgat cggatttatc agtcagcatt tacgaacaat 120taacactacc
attaagagat ggccgctcag tatctctcga tactggcgct agcggttcct
180caactgtacc cgaagtgaca cctggtagcg atccttcatc agaaattaat
cgtttgtttt 240taaaggcaaa cacagaaagt ttacaatcgt taagg
275143271DNADiabrotica virgifera 143atggaactgc caaaaggttt
aaaaccagtg ggacctaatg taactgaaga caccattatc 60cagagtgttg ctacagcact
ccatgtttct tctcaaccag tgacaggcca gacaagtaca 120aaaacactac
ttgaaaaaaa tccaggagta tttctcgatc ccaaacaacc attagtacac
180gctgtaaata tatcagagga tgatatcaaa cgtcaagagg agagagttgc
attagcgagg 240aagaagttac aagaggctct aaagggcaac c
271144275DNADiabrotica virgifera 144atggttcgtc atggagaatc
agaatggaat gaaaagaatc tattttgtgg ctggtttgat 60gcaaatttaa gtgaaaaagg
taaacaagaa gccattaatg ccggaaaagc actaaaaaat 120gcaggataca
aatttgacat tgcatataca tctgtcctta caagagctca gaacacactt
180aattcaataa tcaaagaaat tggccaagag aatttggaaa ctataaaaac
ttggagactc 240aatgaaagac attatggtgg cctcactggc ttaaa
275145269DNADiabrotica virgifera 145tgaatggtgg aggacctaca
gcgcaaatcg tagaaagaga ggggtggaaa catgataaag 60attttgttgg acacaggaaa
gctgtcactt gtgtgaggtt caactccaac attctccaaa 120aacaagaaaa
gaacaactct aaacccactc aatattgttg ttgtgccatt ggatcaaggg
180acagatctat cagtgtttgg ttaacttcat taaaaagacc tcgtgtggtc
ataaaagact 240tatttaataa tagtgtatta gatatgtca
269146269DNADiabrotica virgifera 146tgggatttaa agataaccag
atcgtttata tatcgatagg catgtcgagg aatatgaaga 60aacgggagtt tatggatgca
ccatctttac acccgcctcc tcctcgggaa tgtttaccaa 120cattacttct
tctacagcca aattactttg agcagctatt ttccttaatg cacacactta
180gttcaatgaa aattcaaatt aaaggagggt gccaattacc acatacaaga
gcccaagttc 240tcagcagaag agtatgggat attcttagc
269147270DNADiabrotica virgifera 147tgaaggtttt accatcgcct
actagaacag ctttacccaa atgcgaactg tgggatccag 60aaactatgac cagtgcactc
gatgtcgtaa ttagagcagc aaaacactcg tatcccgagc 120cagtaatgtt
agtgagaggt tttagccagc cagtcaaagt tagttttgtg tatagttgta
180accgattcta tgttcagtta gtaaaaaaag aacaggaact aacaactctt
atggaagagc 240ttcaaagttt atgtctagaa agtgctttta
270148273DNADiabrotica virgifera 148tgtcgagcgc cgataacgtt
accagacgtt cggcgtatct agttgtcctg aaaatatgta 60aattgatact gacatctgtc
gcgcacgtcc tggtaagact gagtgaagat cacactcctc 120cagaaaatga
ggctttcaac gaaaatacca ctccaccggg aacctttttg agacaggcat
180tacgaaatgt tcctggacac tcggatcatt tactgcgaca agtttccatg
aaactagctc 240aaggtttagc gcaattgatg gtatccgaaa cgg
273149275DNADiabrotica virgifera 149tgtttgaagc tcaagaaatt
cacgcagaac ataaagatct cattcacgat gtcgcttatg 60attattatgg gaggagaatg
gccacctgtt caagtgatca atttgtgaag gtttgggatc 120aaacatctga
tggaaagtgg gtgttaacat caagttggaa agcacacagt ggatctgtat
180ggaaagtaac ttgggcccat cctgaatttg ggcaagtttt agccacttgc
tcctttgaca 240gaacagcagc tgtttgggaa gaaatagttg gagaa
275150275DNADiabrotica virgifera 150tgagtgaaaa gagcgtggga
accatgcagg gggtttcggg cgaacgccgg ttgcgccaac 60tggaaatgtt gtttcttggc
gggcctgtgc tagccaaaga acaaagtttt agtatcgaaa 120ccctcataga
tatattactt gttctctacg atgaatgttg caattcgagt ttaaggaaag
180aaaaaacagt ttcagatttt atagaacaag ttaagcctgt agccagcaca
gtaaaatctt 240taagattgac tcgtgaagat ttcgagataa tcaag
275151271DNADiabrotica virgifera 151tgggatatgg caagggagaa
aagattctta gatgtaaatt ggcctgcctc tacaggttga 60tcgacttgta cggctgggcc
tacggctcaa gtacactcat aactgccagg ttgaaccagg 120atgacgaaca
attcttggtc aacccttacg gaatgctctt ccacgaagtg acagcttcca
180gtctgattaa agtagacatg cagggcgcgg tcctcgaaca aggcacaacc
aacttctccg 240tcaacattac cgcatattca ctccacgcag c
271152274DNADiabrotica virgifera 152tggaaaataa actaaagaaa
tacgaaaaaa ttaaattttt aggagaaggg caatttgcaa 60cagtatatca agctcgagat
gtagacacgg acaatatagt ggctgtgaaa aaaattaaaa 120taggtagtcg
acaagaggcc caagatggaa tcaatagaac ggctcttaga gaaattaaac
180tactccaaga actgcatcat acgaatataa tcggtcttct agatgttttt
ggacatatgt 240ccaatgtgtc attagttttc gattttatgg atac
274153275DNADiabrotica virgifera 153tgctctgtta tactcaagag
gatgctaacg cagaaggcaa cgaggaaaac gttctatcgt 60acgaaccagt ccaacagttg
catattaatt atacgagacc tgttattgta ctgggtcctc 120taaaggatcg
tatcaatgat gacttgatat ctgagtttcc tgacaagttc ggaagttgtg
180taccacatac cacaagacct aaaagggaat atgaagtcga cggtagagac
taccacttcg 240tcgcatccag ggaacaaatg gaaagagata ttcaa
275154274DNADiabrotica virgifera 154tgcagatggc tacctttgga
atcgagactc cttctgattc agaggaggaa atagatgttg 60tcaatgtcaa caacgagaag
ttcccgttca ataaaacggc ggtgttttcg ttccccaata 120atccatcgac
gaaagatcgc caacagatcc agatgcgtat ggcaactgct atatccaaga
180agcgaataca agcccaagct caaggcatca agactatcat gcccgttagg
aaaacagcca 240caccggaggc gtcacctatc aaaaggaaac ccgt
274155272DNADiabrotica virgifera 155tggaacaaac tcctattccg
gttgtgaatg gaatcaatag agaaatgtta gacttttgta 60attattcaat taaacgagtt
cctatggaag gcgttgatat gaatctcgat ccagactatc 120tgtgcggctg
tgattgcgat gacgattgca ttgataagac gaagtgcgct tgctggaagc
180tcactttaga gggagcaaag tataccggaa aagatgtaaa tcctacatcg
gtcggatata 240tttatcgaag gttgcccgaa caagttatta ca
272156272DNADiabrotica virgifera 156tgtggcaaac gcaggcagtc
catattacca caaacaataa ggagaaccac tttcaaaaaa 60gtgaagataa gttaactctt
ggaatgactt ctgctatatc aacagcagaa ccaaaaccag 120ttgatttggt
aaaaacaagg gaattaatag aggctctgaa accttttgat gtattcgaaa
180gtgaacaaga attaaacaag aggatgcaca ttttagggaa actgtttagt
ctagttaaac 240aatggataaa ggaggtttca ctttcaaaaa at
272157270DNADiabrotica virgifera 157tgaggataga tcaactattt
aatatcatta tagaataccc agaatctcta ccggctttag 60aagacctacg aatatgtctt
cctagaacag atctaaagcc tatgctaact aagaaactac 120aaaaagcaat
ggaaacacga ttactacacc ctggtgtaag tacacctgat gttctaacag
180cttacgttgc tgccattagg tctttaaggg tgcttgatcc tacaggacta
ttgctcgaaa 240ctgtcacgca accggttcat gaatacttga
270158275DNADiabrotica virgifera 158tggttttaaa taacgttaaa
gaactaaccg aagaagaaaa attcgatatt cagctgcaag 60ctgagcagga actgggcaga
ctagtcagaa cttatacgat cctagaaaga aaccgagccc 120aaaaatgtga
tacaggtggt aaacttctca aacaacgaaa agtgctggac attttcaaga
180aggaacaaaa aaatattctc acggatcttg ctgtagcgtc tgccgatgct
agaagagtgg 240aggacgaaaa gaagtccaag gaattgaagg attta
275159274DNADiabrotica virgifera 159tggctagata cgatttgaca
ttgaatccca tggaagaaac caggtttctt aaaaaacata 60gagtactggc tttaagtttg
gcaagacagg tccattttag ctttcaggag gttgaagcca 120ttctcattat
atattacaag atacaaaaat acgacactta cgatccaaaa ggtattacga
180gagacgttct gatggaagtt ttccattgct gtttagatat gacggatatg
gaaaaagtga 240ttcgaataat aacttgccta gacgagaaag ctcg
274160274DNADiabrotica virgifera 160tggtaaccga gtatgtaatg
gatatcttca aatatcttag agacatggaa ttgaaattag 60ctatcaaaga taacttcctc
aaagaccacg aaactacttc caggatgcgt gcaatactcg 120ttaattggtt
agtcgacgta cacgcaaact ttaaagccac gcttgatact ttgcatatat
180gcattggaat cgtggataga tatttacagg cgaacaaaaa agtaggaaga
aatacgcttc 240agttagtagg ggcctcagca atgttgatag catg
274161272DNADiabrotica virgifera 161tgaatcaaac gtgtaaagtg
tgtggagaac cagcagctgg tttccatttt ggtgctttta 60cctgtgaagg atgcaagtca
ttcttcggac gctcctacaa caacctaagc tcaatatcag 120aatgcaagaa
caacggcgaa tgtgtcatca acaagaagaa ccgcacagct tgcaaagcat
180gtcggcttcg gaaatgtctt ttagtgggta tgtccaagag cgggtcccga
tacggtagac 240ggtccaattg gttcaagatt cattgcttac tg
272162271DNADiabrotica virgifera 162tggctcagag tcctccctta
ccccacgtca attcaaacca agggttttcc caagggtttt 60ctagtattgc cgagtacaat
atagcacaac tagccacttt agcagaaaac acaaacagtg 120ctgccacgtc
accaactaca gctccgttca atattgccga tattttgaac aatgatactg
180ccttcaagaa tattgcaacg ttagttaagc tggcaggaac caatactact
ccgtcgattc 240ctagatccaa caaagttagt cctatgcaga t
271163272DNADiabrotica virgifera 163tgtgggactc ttttactatt
gatgattgtc aaacatgtcc gtataatagc actacaaata 60tatatgacca aatggaagtt
ccacacttgg acaaggaaaa tattcagccg ttcaatcaca 120taaaaaatga
cgagctgtct ctatacgaca atggaggcag tatcacaaaa taccgcgaag
180cacctttgtc tcatactcac cgacccctag aagaacacga ctcaaattcg
caagattctg 240gctacagtgc aagctaccat ggagaaaaat tt
272164155DNADiabrotica virgifera 164gctccaaagt atggtaccct
agtgcctaac agaatattcg tgggaggaat atcagctaac 60acaacagaag gagaattgat
gcaacttttt agcaactatg gtactgttaa agctgctaaa 120attatacagg
acagggctgg tgtgtcaaaa ggata 1551651624DNADiabrotica virgifera
165ggggagtagt acgtagtacg gccggttcta aggtaggcgg ttcgttgtgg
tcgtgtggta 60gtatgccaac cggttggaga aaaatctgtt tttcaccctt tagttctcaa
tagaatgaaa 120tcgtgagttc tgtgacaaat ttcaacaaaa atcgtttata
aaaccatcaa agtggcggcc 180aatacaggat ctcgcacctc caaggataat
atgctcaaat ttctcaccca caaattaaga 240actcattcta taaatgagga
taactatgca gggaaggccg atgaagacca tgattctggt 300acggaatcag
atgaagagat tgatgctcca gtatcaggat cagcatcctg ttcagaacgc
360gtttgtaacg tatcaccggc agacacagga tgttcgatgg aaagtccagc
tcctgatcaa 420cacgatgctc acagctccga agaagagttg gaagttatca
atagcaatag taaaatgagg 480cgatcaacgg cacctcccag gccgctgagg
gctgcgtcag tttgtctagc tgaaaaacga 540aagtggtctc aaattggaga
cgcgcagaat cgaaggtata cacccgaaaa cgaagagaac 600atgtcccact
atagtaatct atctccgtct ccttgttcgc cgttccatat aaaatgttgt
660agcgaagatg gtggacacca tattgaacat tccggctcgt cggatgatga
ggtgcaccac 720ttcttgtaca cacgggctac tcctgtacag ttccgcactt
ctcctccttt agaagcggta 780aaacctgaac gaagatcaat tgtaacgagc
aagggtcggt cgacgagccc gcctgccaaa 840ctacttcact acgaagattc
cccaagaaaa agaaccaagc acatgaaaca tgcacatatc 900cagaggccat
accttgattt ttgaaaaaat gcaacagatg aaaacgaggt cagtaacggc
960ctggcgccac accgctgacc acacaggaga gctgtccgtt tactgttggt
gagatcagct 1020taaggggcgg actccgtcgc cctccgactt aagtaatcac
cccatggagg gctgcagttc 1080gtcatcctca tcatggcctg ataatccttc
atagtctaca aaataactct aaccgaaact 1140aaaactacta aagaattgct
gcatagaagc actgaattgg tacacgtggt ttttggaccg 1200acggttttgg
atttgggtag ttggcgtaga ctggaatact tcaaatggaa tagacgtgcg
1260ttgaagatct tgcgtcgaaa gcatttcgca gactatatag ttttgtaatt
ttatatggac 1320tcattaattg caataaatat tttccactca aatttgaagg
cagaatacag gaatggtact 1380aaaacgggta caactcttaa gatttcttaa
ttaactcaac aagatgttcc taaatactcg 1440gctagaaatc aagtgaatat
aggtacgctg aatattgttt gaacccaaca gtaatgcccg 1500gtttcacaaa
cagctgttaa ttgccttatt cattatgaaa gttaactgtg acagctatta
1560ttaaaggtca tataaatata ttgtatgacc aaaattccgc agaattttgt
agtcaatcgg 1620tata 16241661348DNADiabrotica virgifera
166aaagaataca aagagaatga tagtttgaac atttctgaca atgaaaacat
aagagaagga 60gttactttgg aatggtttaa taagaattta aatgacaccc agaaaaatgc
tgtaatgcag 120gttctttggg gtactgctag acctcttcca tatattattt
ttggacctcc aggtactgga 180aaaacaatta ctcttgttga aacaatttta
cagttaacac gattaattcc ttcatctaga 240ttattagtaa ctgcaccatc
caatagtgca gcaaatttaa tagctctgag acttattgat 300tcaggagtac
ttttaccagg agatcttgtt agattaattt catataatta tgctttaaaa
360gaaactattc ctgataagtt attaccctat tgtgccactg caggcttggc
tagagaagat 420actgttgttt cagaagtaaa taacccaaat ggaatacaat
ttggtagcaa tatgtctact 480ttaggccgaa gtagaataac tgttgcaacc
tgtagttgtg caggaacgtt tttccagttg 540agcattccca gaggtcattt
tactcatata gttgtgacga agcagcacaa gctggagaac 600ctcatgttat
gattccttta tcttttgtaa ataaaaattc tggccaaatt atattagcag
660gtgatcctat gcagctaggg cctgttatat tatctaaagt tgctaccgaa
tgtggactag 720cagagtctta tttggaacga attatgagca gatttccata
ttgtcgtgat ccagaaggat 780ttccagaaac gtctggattt gatccaagat
ttgtaacgaa attattatat aattacagag 840cccttccgga cattcttact
ttgtacagta cactctttta tcataatgag ttgataccga 900ctattgacga
tgaaactagc aatgaagcaa aactgctaac ttctcttcaa gatttcttac
960cagtcaaacg tgataagcta acccgaatac tatttcatgg agtaattggt
gaaaactatc 1020aaacagcaga ttctccatct tggtataatc cccaggaagt
agcccaagtg ttttattatg 1080ttaatcagtt ttacaggctt ggagtaaaat
ctggtggtat aggtattgtc accccttata 1140ttaaacaggc taaagaatta
cgaacagttt tcacggaagc agaatttgaa gtaccaaaaa 1200tcgggactat
agaagaattt caaggtcaag aattcgacat cgtcattata tccacggtac
1260gttcatctaa agattttatc aaatcggatc ttacttacca cttgggcttc
gttgcacacc 1320ccaaaagatt gaacgttgcg atttcgag
13481676699DNADiabrotica virgifera 167gttagattct ttgacaggtc
taattgatta tcgagtgctt tctttgaaaa gctcgacatg 60gagtcccaaa acatgaccat
gatgcactcg gccggaccga ttcagccccc accaagggaa 120ggggcggccc
ccatcccaac agaaggggta accgcaaggg gcacggacca cttcacggaa
180atctaccgac agagaggtta tccttggggg aagaagccaa attgctctac
tctcaagaaa 240tggcctccat gccgagcaag actcccgtca gcgtcctcca
ggagttgctg agccgtcgcg 300gcatcactcc caaatacgaa ctggtccaaa
tcgagggcgc catccacgag ccaatcttcc 360gctaccgcgt gttccttaac
aacgatctgg tggccaccgg aaccggaaga tcgaagaaag 420acgccaaaca
ttcggcagcc aagaacttgc tggatctctt ggtcggaaaa gtgactcccg
480aacaagccaa tcagaccaac ggaacgcccg gagcggctga tatcaccaat
caggtggtga 540catcgttcga tgacaaagtg atgggcaatc ccattggctg
gctccaagag atgtgcatgt 600cccgcagatg gccaccacca ttctatgaaa
tggaacacga agagggacta ccacacgaaa 660ggcagttcac catcgcttgt
catgtgttga aattccgcga aattggcact
ggcaagtcaa 720agaagctagc caagagaatg gcggctcaca agatgtggca
atccttgcag gatttacccg 780tggagggtaa caatctgcct tctacatttg
cgagtgacga agagactctc agcttcaacg 840ccaaggactt caactttgtt
cagttcttgc aggagatcgc tagcgaacaa tccttcgagg 900taacctacgt
ggacatcgaa gaaaaaacat tgaccaatcg ccaccaatgc ttggtgcagc
960tatccacttt gccagtggcc gtctgctacg gaacaggcaa aactccgaag
gaagccaagg 1020gctcggctgc tcatcatgcc ctcgagtacc tcaaaatcat
gaccaaaaag tgaacagcta 1080tcgatacaca gtcatagatc caatcctata
attatccatt tcctgtaatt ctggtttctc 1140tatagttttg tcatacgttt
ttgaggcagc aaatttctca agggcacacg gcaacaaaaa 1200gtgataatgg
atacaaattt gactactgta tagctttctt ctcttggtga acacatactt
1260tattcgtcat cgctccccac gagcgtttct ttagtctgta tattttcttg
tatcacttat 1320tgcgtctttg ctttggtcac tttacgcgcg aaaaccatta
tgttttacta atttttcaat 1380aaagtacact ttatttaaga acaggggaga
aaagtcacga gaatttgaaa agcgagcttc 1440atgatcccct gaagaatata
tcttgaagaa ctattatttt ttaatttttc atcacagtga 1500tgtttgtttc
agaacaaacc gggaaaagta ttatgagaat atgaaattta aaagaaaatt
1560tgaaaagtat catgagtttt gtgattctcc gaagagtatg tctcgaaaga
cttcgacttt 1620ttactatttt tgtatgcaca aaattcgttc tacatatgaa
acatgtcagt tatatattca 1680cctttatatg tcacattttg tggttcttta
acgaaatgtc aagagcataa agtatgtcta 1740gaacgatttt acctttgcgc
gaaaagtgac cacaagcaat gtcaaagaga aggatagcaa 1800ctaattttac
aagctatact ttgcatagaa ttagtggttt ttttctaaaa tcaatttaca
1860tattattttt gacttgaata aatttttaaa tttaaaaatg cgtgtgtgca
ggtatattta 1920tcacatcatt cgtcgtgtac cacatctcgg atcttgctac
taatattcga ttcgataggt 1980gctttgtctg gttaacttta gtgggaaggt
ttaggtattt gtggtatctg tcgatgttgc 2040cagaacgtaa gatatcctaa
actcactttt ttccgaaaaa atatcgcaat agctcaaaag 2100ggaacaattt
ttgtcgagtt attgatttcg aggaataata cgaacttcca tagattcaaa
2160tgaagtaaac aacatacgtt tttcatttaa aatgaactca catatgcaat
gcattcaaca 2220ttccatagat tcagtgaaaa cacgccatcg ggttctattt
tgcgcttagg ccttcacctc 2280gtttcaaggc tatccttaac atcttatctc
gtctatgata tatcttctcc aagtttgtgc 2340tgggcaaatc aatcaatttg
cctcgaaatc taaggttata ttacttttat gaatcgtgat 2400aacatgcata
aaatataata aaaatttata gttttttgca tttttattcg gcttattgta
2460atattcctcc atagttctta catccttgtt tatttccctt tttgtggaga
ggtactatgt 2520gtgtttggct agattctgtt tgacattcga aaagtaaacg
tcagtttgcg tttcaatgca 2580tcgataagac gaatggtaaa gctgctaaaa
aagcaggaaa agcccagaag aacgttcgac 2640tttcacttca ttctatcggc
ctttcttctc tttgccgtat tttattttgt aaggtgaggt 2700atttgtacat
gaagttgtct gcccctttct taatcggttc tgcatgaatg ccatctgggc
2760ccggggctct gtgatttctt agtgtcaata gtgctgccgt tacttcctca
agcgctggta 2820acgctttatc gggttctgct aagtggtaag tagaaggtaa
gccacttaag cacacatgtt 2880acataaatgt caaaatttcg caaatatata
tcacataatt tgtttgtgga cataaaggaa 2940agaaaaaaat gtcaaaattt
tgcaaattat gtggcatttt gacatttatg tgacataatt 3000tgcgacattt
gacgtacggt tcaagtagct gaagtaaaag tgtgctccat tggtttatcc
3060tctactgtac aactgtttac ctgtgtatga taaataaact tttttcgggt
ttggaaaaat 3120ttcaaattgt tcaggtaaaa tcttgcttca tgattgaaaa
tttgcaaggt ttggaggagt 3180tgtactaatt tgtgtaattg aagttcgtat
tatttcctcg aaatcaataa tgcaatgtgt 3240ttaaattttt gtcgagtcaa
aagcgaagaa tatttgaaaa tttctaatat tgcgatattt 3300ttttatcgat
ctggcttcaa tgtttgaatg atgattatat aacgagtaga tagctttaga
3360ggtaatacac tattattagg cactagttat taattataat catcaatatt
atctatcgat 3420actgaatgta attttttata tagtgccccg atgatatgat
atgtatttat tatattgtga 3480aaagggagca tttatttgat gcaaagtata
gaggttgtac ccaaactttt cggggtgcaa 3540tcttcttttc cggaaaatgc
tcgaaaactc aattggcgca cattcagcag gttttttaac 3600agtttgaaaa
ccttgataaa tggaacgaaa ttaaacacgc tgagaaagct atggttgagt
3660gaaaaaggtg ggaaatgttg actgttgtag ctgattaagg ggtgtattct
atatttcggt 3720taaatttaag aggcttttaa aatttaacgt ttagcagtct
tctttttaga ataacattga 3780cattctataa agacgttaga gaggttcact
aaaaaatatt aggaatccac tacaaggttt 3840tcaatagggc attctaacat
gtcaaaatta aatcaatatg gcggctgacg ggcaaacaaa 3900tgttatttgt
cagttcttaa tatggtttta gattcgtttt tagttgcctt tctttgtaat
3960tttgttttgt acaatgatta atttaacatt ttcagtggaa gaattaattt
aaaaagataa 4020tattcctcat ttctgttgtg ttttgaggtg tggaatgcga
agtaattgtg atacaagttc 4080agttttatca gttaccatga gtgcaaattt
tgagtgaaat gagagaagaa cagtggttaa 4140atgctataaa aggtcctata
aaaatgctaa ataactacct gtgccatgct agtagtctac 4200caacaacttt
caaaaatatt cactcatgtt catttattca ccggttaaaa ctaaataaag
4260tcgttcaagt aattctacaa ctaaaaactt cacaaaactg acaacaaaat
gttcggaaaa 4320tacaactaga aaactattaa aggtaaaaaa caaaatgtgg
tacaaatata aacagagaca 4380agttttgtat ttccgaccat attggtgtca
ttaacgtcaa gtctcgaaat gccctgttag 4440atacagcaaa ttgatcgtgt
ttaaactact tgtaatgtag aagttgtcca aaaactgtca 4500gaggccaaaa
cacttaaaaa taagaaattg acatgaagtg aagacttctt ttttagctaa
4560atctattgta agaaatcctt tcgcaaaaaa cgatcgcaga aacttttcca
tccacatcgg 4620ggcggatatc agagaataat aacttaaata gtagtagatt
ttgcgttgtg gtgaaaaaaa 4680aatagagatt ggaacacatt aaaaatattc
ataaatctat tagagctaga aattttaagt 4740gtaacacatg tggtaatttt
ttaaaggaaa tcactctgga gcatcctagc gtgtgttcta 4800cgatagttca
tcatagtact ctcttttttt tgtaactcat tttcccatta accattttta
4860tttatcaaac tatttaatca taaagttacg attttaaggg atatagaata
caaaaatatt 4920ttaacagacg gtaaaatttt agaggcctct taaatttaac
gaaagtgtta cagaatacac 4980ccctaaggca agattaaaaa acggcaacga
aggaagagga aattgctgga aattttagta 5040attctcacat tgtttccgaa
aactcaggaa attatctgaa aaactgcaac cgatatttca 5100taatgttgcc
agatttctgc aaatgattgt attttacctt ccagtattcg cgtagatttt
5160caggagaagc atagttgcat cgagagaccg atattaattg aacgttagtt
tttgaaaaaa 5220aaaagcccca acaacttggg cttttaggat gaataatacg
tacatattct tcaagaaaca 5280gaaattttaa tcagataaac taaatgtggt
attactgtta atgcagagaa ggttcctatc 5340ttcaatgaga tgatggcgac
cacattgacc catcttattc tattcacatc agtcttgcct 5400attcacaatc
ttgccttgta gaatcaactg tatgaaaatt aaaaatctaa tttttatatt
5460gaaatggtgt agaagacaat gcttaaaaat tgagaaaatt gtcattacaa
acaacggtat 5520ttgaagatgg atcgtatgaa gacgaggata ccgaatacac
agtaaatatg tgtaaataag 5580atcacgagaa ctagacgaaa aacacagaaa
ggaacttagt gaaaacaata gttgatcaga 5640accactaaat gttagtatgg
aaaacaggga agaagatgaa agtgaaatga ctgaagatca 5700accagtagga
aatggcatta ataatacatg tatatcttga gcgcagaaac gacgaaataa
5760aaaggaactc gaagcaaagg aaaggaaaaa tctaatttta gagcaagcta
agaagaataa 5820agagggaccc agaattgtta aaatccttaa aatcagctgt
agacgcagta ctaccataca 5880acgtgacagt gtgacaaaca aaatttcgac
caatcaagtg ccaaatttca tacaattttc 5940gatacaagaa cttgcatatt
gttatacagg gcacaaatgt acgtacaaga aacgatacaa 6000gaaaatgtat
atcagaaatg atattagaaa tgatacaaga aaatgcatag tgtcatacag
6060ccataagttg gtaaatgatt taagaaaagt tacagcagat tttgtaagac
aaaacaaaga 6120tgacttcaaa ccatttatgt gcaatgaatt aggtgaatcc
gaagtagtta gcgaggaaca 6180gtttgagaat tattgtaaag atgttgcaac
aacaaattaa tggggaggtc agttagaact 6240gcgagcttta tcgaatattc
ttacttactc cattgaagtt attgaggccg cgattccttc 6300cacactgtag
gaaaaaattg cagtttgctc actttcatca atatcaaaaa ttgaactttg
6360acccggtctg ccagaccgtg cgcgactact ggaaggttta aaataccttt
ttatcgcata 6420ttccccataa aagtgaaatc tggcagtaca gtgttgcgaa
attgagggct ttttctggaa 6480aataagatta tgctcttttt gatttttgtg
aacggtgatt gtggaagagc aaacacctcg 6540actggttctt tgggtataag
cgacggtgtt tcctcgtcgg caatcgctgg gttctaatta 6600acatatatgt
caatagaaag tttttaggca caaaccgtca atacatatag gcatgctatt
6660gtcgaagatg tctaaagatc cctagaactg tagaattgt
66991681463DNADiabrotica virgifera 168cctccgaaca gcattatcaa
aaaatgaatc caccgaaaat ttagacatac aattagcggg 60atcttcgaaa aaagccttgc
tgtccaatat cgagttcgaa tcggctggga gctatcataa 120tactgtctta
gataggctaa aatcgaaata tatagtgctc agaagtcctg aatcaccacc
180cccaagtaaa cctgaagatg acatgaactc cagtgaatcc ttaaaattag
caaaatatga 240actatttcca ctgagcgatg tacaactcgg ttggaataac
tgtgattggt ctgttggtgc 300tggaatggtt aatatgggaa atacatgtta
tttaaactct actctgcaag ctctctttca 360tgtaccagct tttgttaatt
ggttgatgtc agacaaagat catactgcca tctgtcaaga 420ttcaggaggt
ctctgtataa tctgcgcgat gcgcaaaact ctccaagact cacaacaacg
480taacgccaac tcgatccgtc cattgttgat ctacaacaaa ttgagattgg
tgtgtcgaaa 540cctcataccc ggtcgacagg aagacgccca cgagttcttg
agatatctag tcgaagcgat 600ggagaaagca ttcttgagcc ggttcaacaa
ttataccgaa ttcgactcga aagtcaaaga 660aaccacccct ctgaatcaaa
ttctcggggg gtacctgagg tcggcggtta ggtgcttgaa 720gtgtggccat
gtcagtacga cgtttcagca ttttcaggat ttgttgttag atataagaaa
780ggcccaaact ttggacgaag ctttagaatt gtacttttct agagaaaagc
ttgacgacga 840atcttatcag tgcgaatctt gtcagaaaaa ggtaccggcc
acaaagcagt tttcgataga 900acggacgccc atggtgcttt gcatccaact
taaaagattt tcagttagta acaataaaat 960aacgaaacac atcaacttta
gacaaagact ggatctaacg aagtacgcta gacatcgccc 1020taacgtacct
ctgatctaca ggctcgtagc gttggtcacg cacatgggcc ctaccgtcag
1080ctgcgggcac tacacggccg tcgcgcaagc accttcaggg aatttctttc
aatttgatga 1140cagtatggtt agaccaatat cccaccaagc agtgttcaac
accaacgcat acattatgct 1200atatgagttg gaatcctcgc cctactcacc
taaatctgcc cccgccacgg taactagcaa 1260actgaagact tcaaccccaa
ctgccgaaac cagttcagtg gcttgttcct cgaattccag 1320caccgtaaat
tcggtgaccg cctccacaaa gtcgtacgcc aacggcattg ggtttactag
1380cgggaaggtt tatggtccag aactgcctcc ggataggata gagaatagtc
ggaatggata 1440tgtgacaagc agtaatggta aag 14631692813DNADiabrotica
virgifera 169aacagcaacc tggagcgaga caacagcagc atcaaactgt tcccccacaa
cagactggtc 60ccccacaaca gactggtccc acacaacaga ctggtcccac acaacagact
ggtcccacac 120aagggcagca aaaaggtgcc caacaacagc aaacgggtcg
aaatgaacgt caacaacaaa 180gttccccacc aaagcaaatg ggtccacatg
gagatgtacc acaacaacct aaatcggcaa 240acgggcaaca aaggggtgga
ccacaacaac agcgtaggcc tcaggatagg cagcagacag 300cttctgttaa
agaattgaca tctcgtatga gtgaactgca agctggtcca cttgtaccta
360tgaggttaag aaaccctgaa cctggaaaag caggtcgcaa aatacccgta
gaaacgaatc 420atctcagcct cgcgctaggt aaattaaaca cggcttatca
ctatgatgtt gatttggtac 480cagatacccc gaaaaagttt ttaagaaccg
tgatggaact ctttaggcag caacattacc 540caaaaagata tccagccttt
gatggaagaa aaaatttata cagcactact atgttacctt 600ttggagaaca
aaaagaaggc gaggttacta ttcacgacag tcaaaataga gagaaaatat
660ataaagtgaa agtcaaattt gcaaattcgg ttgatttatc ccccttgaaa
gattacgatt 720tatctagagc aactccgacg gaagctatac aagtggtcga
tattgtttta cgttgtgctc 780cttcacaaaa tctacttcaa gtgggcagaa
gttttttcca taagccagtc ggagaaatta 840tcgacttagg agaaggcatg
gaaatgtatc acggatttta tcaatcggcc attcgaggct 900ggaagccact
tttaaatgtt gatgttgctc ataaaccgtt tcctaaagct ataccagtta
960tagaagcttt gcttgaagta ataaatgcag acagatataa taataaactt
acgagaaatg 1020accttagcag acctcttgat aggcgagatt tggaagtttt
cggcaagtac atgaagcagt 1080taagagttgt ttatgaaatt ccaaatttgc
ccagttctag aagaagttat aaggttaacg 1140gtataaatga cccaccggcc
gtgaaaacct ttaaagatgc gaataataga gaaattacca 1200tccaacggta
ttttgaaaca gaaaaaaggt gcaaattaag atatcctcag atgccgacac
1260tatgggtcgg atcttcagct agatcggata atcctatact tgtacctatt
gaattgtgta 1320caatagaaga taatcagaca attaatagaa aaatgacaga
aggacagaca agaaacatga 1380taagatatgc tgcaacctct actacagtac
gcaagaataa gattatggaa ggaataacac 1440gggctaattt taacaaccat
cccactgtac gagaattcgg tttttcagtt tctagcgcat 1500ttgaaaaatt
agatgccaga atcttgcccc ctcccagatt aggctatgcc ggtaaagaag
1560tgaatgttga taaaggaata tggagaggtg acaaattctt ccaagcggtc
acgattaaca 1620aatggacaat cgtgtgcgct gataggaggc caccaagacc
agatgactta cggaacttag 1680cttcacaatt acttagagaa gcacgcggaa
gcggtatgca gattggtgaa gcagaacaac 1740cattctgtac aattggtgac
agaaatatgg acatcaagaa atacttcaca tctgttaaag 1800gcaaatatga
tgtaatcttt gtcgtcgtac caaatagcgg accacaatat agttacgtta
1860aaacagcagc tgagattaat gtaggctgtt taacacaatg tgttaaagta
agaactgtgt 1920taaaaatgaa ttctcaaacg gcgttaaacc ttttacttaa
ggttaatgcc aagttaaatg 1980gtactaatca ttttctatct acccgtccgc
ccattttgaa tagaccaacc atgatcatgg 2040gcgctgacgt cactcatccc
agccctgatt cgcaacatat accgagtgtg gcggcagtca 2100ctgcatctta
tgatccaaaa gcctttaagt acaatatctg ctggagattg caaccaccca
2160gacaggagat catcgaagat ttggagaata ttgtggtaga ccaattgaag
ttcttttatg 2220agtctaataa aggtcaaaaa ccccaaagaa ttatcttttt
tagagatgga gtatcggatg 2280gacaatttga acaagttaca agtgccgaag
tacgagctat tcgagcagca tgcaagagag 2340tccaaagaga aggttatgaa
ccggcaataa ctttccttgt tgtccagaaa cgtcatcata 2400ctagactttt
cccattgaat ccaagagatt ctcatgacag aaatttaaac gttccagcag
2460gtacttgcgt ggacacgcat attacacatc catttatgca ggacttctat
ctagtttccc 2520acgccagtat acagggagtg gcgaaaccaa caaagtattg
taccttgtgg gatgacaacg 2580atatgtcaaa cgacgacatt gagcaactta
cgtattttct atgtcacatg ttcactcggt 2640gcaacagatc cgtaagctat
ccagctccga cgtactatgc ccatttagct gcggccagag 2700gcaaagtcta
catagaaccc gaaaatgtgg atttacaaaa tttaaatagg gaatatgaaa
2760gatttaaaat ccaggacagt atccagaagg gactgcctat gttctttgtc taa
28131704075DNADiabrotica virgifera 170caaaacaatc ggagctgagc
tacgcctaga gtcatggttc aaaacgagta ctacgaaaag 60gccagcgggg caaaggccca
cttccacggg gcaggcagca cgtcccaatt cggagataga 120tacaggagac
gctgacgatg aagacctaaa acttatcgac gtcatgagcg agaaggaagt
180taatgctaag atcgaggagt tattgagtga catgaacctc aacgacgaca
aaaagaaacc 240ccttagagaa atgcccatgg aacagaaacg tgcaatgttg
aagatgcaga acaaaaagaa 300aggacaacac gacacgggca gtcgattcaa
cgatcccgaa gattattaca gatatctgga 360tcaacacata aatccggagt
acttgcagtt gaacaaatgc ctgaactgtg tggaaagctt 420gagggtagcc
ttgactaaca acccagtcag ttgggtagag aagtttggga ctataggagg
480tttacagaaa cttgaacaag tattggatac gggaataagg aataaagatg
ttcgacttca 540aacagaatgt ttacgatgtt tagaaaaatt catgaacaat
acgacgggac ttaaagcttt 600tttcaaattt tcaaaagcgc atttgactgt
agccagatgc ttggaccacg aaaagcctac 660tgtgatgtta caagccttaa
agattcttgc acctctatgt atcctgaact tggaaaagag 720cggcgaggaa
gaaggtgtaa aaaaggtgct tagagccatt accgaggtgg gagagataca
780ttcgagagaa cggttcctat ctgtggtact tggaatcact gccagcgata
atgccgaatt 840acagagtatg tgtttccaat tcatcaatgc cttattaagc
gaaacggagg acttcgagtt 900ccgtatgcat ttaagaaacg aaattgtaag
aaacggactt tacgaaaaac tacaagaact 960aaggaacgag agtaatcccc
aactaaaaac tcagttcgaa atattcgaaa acagacgaga 1020agatgacgcg
gacgaatggc acgaacgatt tgacaaggtc aggctcgaca tggacgatat
1080tcaagattgc ttcgaggtct taaaaaatat cactctagat acgccgtcgg
agccttattt 1140tttgagtatc ttgcaacatc tcttgttcat taaggaagat
gtcaatatca ggccagctta 1200ttttaaatta attgaagaag tgataacgca
gatagtactc aataaggcgg ggctagatcc 1260tgattttaag aaaaaacata
tagacatcga ccttcagccg cttctagagg aacttaaaga 1320caaaccaatg
caaattgaaa gtgcagaagt cgagagccta aaaaaacaac tcgaggaagc
1380aatcgcagca aaaacagagg cagaagccaa actagaactc gtccaaggca
gagcgggtac 1440cacacagcct tccggaaaac tagatccagc tttagtaaac
aaaatcaatg tgccaccacc 1500tcccccaatg ccaggcgccg gtgcgcctcc
accacctcct atgccaggaa tgggaggcgg 1560tcctccccca cctccgatgc
ccggaatggg tggcggtcct cctccacctc cgatgcctgg 1620aatgggaggc
ggtccacctc ctcctccaat gcctggtatg ggtggtgggc caccaccacc
1680tcctatgatg cctggaatgg gcgggggacc gccgccgcca cccatgatgg
gtggtatgcc 1740tccacctcca cccggcatgg ttcccttacc gggtttggcc
aggccggatt tgttaccaca 1800cggtttaaaa ccaaagaaga agtgggaagt
tacgggacca ttaaagaggg ccaattggaa 1860aacgattcta ccacaaaaaa
tgtccgaaaa ggccttctgg gtaagggcaa aagaggaaga 1920ccttgccgaa
ccagatattc tcgacggttt ggctaagaag ttctcgtcta aacctgccaa
1980attaccagaa gatgtgacag ataagagcaa taatacggga acgatgaaga
aagttaagga 2040acttaaagtg ttggacggaa aaactgccca aaatattagt
atattattag gtggatcgct 2100caaacacata ccatatgaag atattaaaaa
cgctctactc agatgtgacg aagctattct 2160atctgataat gttactgaac
aacttattca atacttaccc cctgccgatc aattaaacaa 2220attccaaaac
ttcaaagagc aatacaaaga cctgaccgag gctgaacaat tctgcgtgaa
2280gatgtccgaa gtgaagaggc tgttgcctag gttgaaatct ctcagcttta
agcatcatta 2340cgggaaaagg gagcaggata tcaaaccggg aattgttgct
gctacagctg cttgcgacga 2400agtgaagaag agtaaaaagt tcgccagaat
tttagaacta attttattga tgggtaacta 2460tatgaatacg ggaagtaaaa
atgcgcaagc gttcggattt gaaatgtctt tccttaccaa 2520gttaacctcg
acaaaagatg tttcgaataa gcaaacgctt cttcattaca ttactgaaac
2580gatagagaac aaatttccag atctgcttaa tttttacgat gaaatgcctc
atatagatca 2640agccagccga gtatcgttag atacgataca gaaagcgtta
caacaaatgg acactagcat 2700aagaaattta aagacggatt tgacaaataa
cagggtacct cagagtgaag aagacaaatt 2760tttagaagtt atggagaaat
tcgccgaaga agccagagaa caatgcgaca tcatgcaaaa 2820aatgttaaag
aaagtagaaa acctctacag cgatctagct gaatattacg tgtttgacaa
2880acagaaatac gcattagaag aattctttgt ggatttgaaa acgttcaaaa
atagtttcct 2940gcaagccaaa gccgataatc agaaagaaaa ggaaatcgaa
gagaagaaag agaaggcgag 3000gttagcgaaa ctaaaacaag aaaaagaaaa
ggaagagaga aacaaacgaa ggctgattga 3060tatgaatcct tcagaaacgc
aagaaggagt gatggacagt ttgctagagg ctctttctac 3120gggtagcgca
tttggaaggg agcagaaaaa gcgaagaggc catcgaccag caggagctga
3180aaggagggcc cagttagtta gatctaggtc gagaacggcc ttaatagctg
gaagagaatt 3240aactagtgaa atattggcgt agcattaagt aataataaag
aagaacgcac aacgacttga 3300acgctcaaaa gaaatatatt atataaagaa
atattttata cgttgtaata atttattttg 3360taaattgttt ttccatttgt
aaataataat ccgtatatgt atatttagaa attgaacgaa 3420tgaatctctt
atatatagtt ttagttttat cgcagattat tatgtaaata tgataaaaaa
3480ataaaaaaaa tcagtctttt gttaaacttt atattgccgt aaaagtagat
tagtttttta 3540tatattgttt ttgaaattga acaaattctt gtacgttttg
aggtagtgtg cttttataat 3600atgtaaaatg ttgtaattat tatatacttt
actgttcgaa tttttgttag gttttgtatt 3660ttagttttat ttatttcttt
aaaaaaaaaa tgtgtatggt taattaacca aatatatgcg 3720tactgttttg
tttataagtc attttaagtt aagttcaaaa agctctttaa ttggacctct
3780gttcttgcta ttttttgtag tgggtgacat caaatacaag atggcagata
agtttatttc 3840agaacagaat tttcattcct aaaaaatcct ataaaaccaa
ttaaaagatc tgttgcgctg 3900ctatgatgtt cttcctttca ctgcactaac
agaaagagta gtaccctgta ccagtatctt 3960cctggatcat tcactctccc
tggaccactt tttgttacaa aaagaatagt actagggagt 4020catcattatt
tacgtttgtc gctatacagg atgttagtga ataagtgtga gaaac
40751712294DNADiabrotica virgifera 171cttctaccat tgatagtgct
atttataaat aaaaatattt tgtgtttatt agaaaaaaac 60tgttttatat taaaaaagag
aaactgtata gtagtgataa agtgtatggt gcaaaatgct 120gttttctaat
gagatctggg cggctacaaa acggcaggca ttgaaaagga cgaattcctg
180ccaggataga attgaaagga tccctcctag ctcggaagaa cttttcgaaa
atatcccttg 240gatgaatatc atggctgatc tgactgttaa atctgctata
gctgaatatg agtttaaacg 300tatgctgaat aaggagctgt ctcatttctc
cgaatctagc aaatctggca atcaaatatc 360ggaatatatc tgctccactt
tcctagacaa acaacaagaa ttggacctac cctcccttcg 420tgatgaagtc
cctgagctcc tcccaaaacc cactcaacgc aaagaacgtg cccgaggacc
480gcattctacc atgtctcaaa tatcaggtgt taatcgtaaa ccactttgcc
acaccaattc 540ttttaccggt gagaagttac ctttgcacgg cattgaaaca
ccttacgaag aagaattagg 600caaaagtttg attatgatag atcaatgggg
tatcgatatt ttccgcatcg gagagttaag 660caacggaaag cccctaacct
gtgtagctta cacgaccttt aacaatagag acctcttgaa 720gacaatgaac
atcccgccga agacgtttat aacttttatg atgaccctag aagaccatta
780tgtgaaagaa aatcctttcc acaattccct gcatgcagcg gatgtggctc
aaagtacaca 840tgtactgctg aatactcctg cattagaatc tgtatttaca
ccgctagaag tgacagcagc 900tctattcgca gcgtgcattc acgacgtaga
ccatcctgga cttaccaatc aatttctaat 960taattctagt tctgagttag
cccttatgta taatgacgaa agcgtactag agaatcatca 1020cttagctgtt
gcctttaaat tactttctaa cgacggttgc gatattttct gtaatatgac
1080caaaaagcag agacaaactc ttaggaagat ggtcatagac atggttctta
gtactgatat 1140gtccaaacat atgactcttt tagctgatct aaagactatg
gtagaaacga aaaaagtagc 1200tggttcaggg gtacttcttt tagataatta
cacagataga atacaagttt tggaaaattt 1260agtgcactgt gcagatttaa
gtaatcctac caaacctttg gatctctata gacgttgggt 1320agatctcctc
atggaagaaa cttgggcaga tctagtgcat cctgacgctg cagcaaggag
1380acaaagaaag agaatccaaa atggatatca gtcccatgtg cgataggcat
tcagctacaa 1440tagaaaagac ccaagttggt ttcatagatt atatagtaca
tcccttatgg gaaacttggg 1500cagatctagt gcatcctgac gctcaggata
ttttggacac cttagaagaa aaccgcgatt 1560ggtatcaaaa cgctatacca
ccgagccccc ctccagaaga gactcccgag tcccagcgac 1620caggcatcag
attccaagtc actttagaag aaggcgaagg ggaatctgaa gaaggtccga
1680tgtgacggaa actagggggc ctctgtagaa agctcactga aaaggtgcag
caatggtggc 1740gtatcaggca gtaagcgcaa gtgcaggcgc atgcgcactg
actgacgaac gttacaccgc 1800gcaggcgcgg gtttgttgtg ttttgcaata
tatgcttgac tgaaattttg aatttttcgg 1860gctgagatgc cagcctgttc
ttatatatcg cagagttcct gggagtgtac tcttttttca 1920attttttgtt
cggtttttta gcaaatctgg tagatacatc gcttcatggc caaatcgtaa
1980gcttaaataa tctaggaaat acttttcaac agattcataa ccatccctaa
acataaacta 2040aacagttttt aagaaaaaat agtaatttgt aaatctttta
tgtttatgaa ttttgaaacc 2100gtattgttaa atgtaaacca tgtaaaagta
ttgttaattt caaagtgact gtaaacagac 2160ttaaaatagt aagtcgattt
acagtgtgac aatacaaata taggcatgtc aatgtagctt 2220tttatttata
tgaaatagcg gtataaatct tcaaaacatt aagataacta cagtaggaaa
2280aatgaaagaa tacc 22941723145DNADiabrotica virgifera
172ttttgataca caatatctct tgcgaattat atcagaaatt ttacaaagct
acttttttcg 60attttacttt aaattaggag gtaaaaagta attgtctttc attatatcaa
aaagacatct 120gagcattatg cattacaata tgacagcatt tttggggaaa
aaatatgaac gtttttgttt 180tctgaaaaac cgtcatattt ttttccaaaa
aatatttgat tggtgtaata gcctaaatat 240atttgaaaac gctaaatact
ggcgaaaaat actaaataca aactaaatga taatttataa 300tttatcacaa
aaattccaaa atggtcttat ttaagtgtcc agtaaactag tgctgctaat
360ttggagatag gactagctca caccaatcat agcttaaata aaaaaggaaa
gcagacttta 420acacctcatt attttaggtt ataaaacaaa ataagtattt
gaaatttatg tcatagttat 480aaaaaaacat ggtatagttt tttactacaa
aagtcaggtt aagtcatagt ctatacaaac 540tgacgtcact tgtagttctt
caatgacagt ttatacaaac tgatgtcaat tatagttctt 600caatgacagt
ggggcgattg gaaaaaacgt ttacagcatt gccaacaatt tatagtaaga
660attgtgtttg ttttgatggc atctacacca acgaccaacc aatgcaccgc
cagactgaga 720gttttgttta cgctgacgac ctcggtatcg ccgtaaaaga
gaaaacacat acacaagtaa 780agtcgacaat ggaagaggct ttaaacgtga
tgtcaactta ctacaaacaa aactcattaa 840agctaaaccc tactaaaacc
caagtatgtg cattccatct caacaaccat ttggcgcatg 900gcaaactgaa
tgtatcttgg gagggacaac gcttggaaca tactgatagg cccaaatatc
960ttggagtgat actagactgg tcactaacat ataaattcca ctgtccgagc
acaagacaaa 1020aggtttctga gagaaaaaga cttctccgga aacttgtagg
gagcaagtgg gtgcaaaccc 1080ccaggtgtta aagtcaatag ctgaggcctt
atgtttctca acaggggaat atgcttgtcc 1140cgcctggggt aaatctatgc
acgcccaaca agtcaccacg gcgttaaata aaacatgtag 1200aataactacc
agttgtatga agcctaccct tctaccccta ttgtatcggg cagctggatt
1260cgcatcacca gatgaccgta gatgcgcctc acaatgtggg gaagttgaat
gaaaggcatt 1320aattatacga gtttgacgac ccgtcaggaa ccagccgact
taaatcaggg aaatagttta 1380tgagaaatgt cagcgtcaaa ctacccgacc
tgttccctct acagccagaa cgacctaatg 1440gaatgaacct ggactggaga
aactggcgga cacttaaccg catacgcacc ggttttgtcc 1500ctctaaaaca
gaacctcatt aaatgtggca tcaagacaga taacgacgct ctttgtgaat
1560gtggggaaat acaaaatttt gtggttctga cgtcagaaaa cttgaatgat
gtaagttaat 1620ttttgtagta aaaacaccat agttggacaa ttactataag
agaaaaaata tcacaatgtt 1680agtaggtttt tccatttgac tttctacaga
aaaagcttag taaaatgcaa tcaactaaaa 1740gttcacttac actgtctcat
ttttaacaaa aaaaatgttt tataacctaa agcaaaaggt 1800tttttttgaa
aacttaaaaa gccaataaac aaatttcact ttacactgtt aaaccgtata
1860aatattttgt gcttactcta accctttttc tgataaattc cgtttttaga
tcccacatcg 1920gcactattac aaaaagcaca ggagcccagg agaaaggaga
agggtcgaaa ataaaataga 1980gacagcagga ctagagattt ttttattaaa
aaatcctaaa ctgtaagttc ttcagtatca 2040aatgttttta gagatagcaa
tggtatgaga aaagcttaac aagaaaaatg cgctttatta 2100taattaaaaa
aatcacatca tgttccggaa acttatctca gttaagtttg cgaaacactc
2160tcaaaaaaaa acttaaaata tcctaaatac tcttaaaaaa caatgataat
tgagaccaca 2220gtcataaatt atgattggca aattggcgca gtcgtgttag
gaggtcacgt attgtataaa 2280aggttttaaa agacaaattt ttttgttata
aatttaatat tatgaccctc gtaacataac 2340tggaaccaca cgatggacaa
cgatgaaaaa attaatgatt ttgaccgata tataatatat 2400attttatgtt
gtttgtttgg taaaaaaagg aaaatacaat attgtccgat tctatgactt
2460ttttccatgt attttttaat gatgatgcac tcggtatacg cattgccggt
tcgctgttat 2520tttgaaaaaa atacaaattt aataataatt aatttttcgc
cgcacggcat ggctgcttac 2580agcggcacac actatctcta gtaaccgcag
gtaattaagt aatcacctag cttttctacc 2640actgacgctg cttgctgagg
ttggattgga tcttcataaa gggatactac aacagcttgt 2700gtcgttttca
tgcaatgtac accaactttg cccaatttcg ccctaattac acggtctgtg
2760ccagagaggt atatgtatct gttaccggct aatgttacgc ctgaggatgt
gagaatgtct 2820tgtttttcga agccctgaac taattttgct agttcttctt
ttgatacatc gaaattttct 2880gatttagccc aaacgtttcc gtcgtggcca
gcgattgccg ctttcgtaac gcattttgaa 2940gccaagagct gtttgtctac
gtaatcctgc cagctcatat tgttgtaaat ttaaaaaatc 3000actgcacaca
cggttctact cactaataaa attggtgcaa ttagagcagt tgccgcacga
3060cgaactgaag ccgaatactt ttgtctacca aggaagattt tgtagtggca
gcggaagctc 3120taaagagaca agtgcgactg ccccc 31451731208DNADiabrotica
virgifera 173aaaaggatgt ttagcacaca attaggaaat tttccatttg ctaatactag
gaacagccaa 60gagacaactt ccaacagttt gaataatcat ataatggatg ccaacaatgg
agctggagat 120agtaatgatg gttccataga taaaaccaaa ctaattatta
attacatacc acaatatgcc 180actgaaggtg aactaactca aatattttca
accgttggaa aactggaaga tgtaaaaatt 240atgagagact ttaagactgg
ttacagtttt ggatttggtt ttgttaaata ttccactgaa 300gaagaagcag
ccaaagcaat tgcagttcta aatggatata atttgatgaa taaaagatta
360aaagtctcct actcacgccc accagggaca ggcatgaaag attcaaatct
ctacattaca 420aatcttccaa aaaacattac tgaacaagaa atagataaca
tatttaggaa atttggcgaa 480attgtacaaa gaacgctgtt aaaagataaa
attactggaa tgccaagggg agttgctttt 540gtaagatttg ctaagggcga
agaagcacaa gcggcgatca atggcttgca tggaacaacg 600ttggcaggtt
cgatgctacc tcttagtgta agagtagctg aagatcatgg aagacagaag
660gcacagtatg tggacgtgtg ggatccgatg ggttttaata gagaatctga
agaatcattt 720tcatttggag acgaaggtcc tgacttagaa gaaattctta
ttactgaagt gtcatataga 780agacccctct gggacttatc aattcactcg
acgaaaatgc aaattgacaa tctttgggag 840cagatctatc gtgtatttaa
gaataaatat tcgataggtt ttctaactaa catgtggtct 900aatcttaaag
aaggctatat aagagataag agatcatttc cgtattacta tcaaatgaga
960tttttggatg agtttgaaaa tgttgctcta tgtggatcta gtcgaaggca
tacaggaagt 1020cctttaggaa aaaaattaaa aatggattac cagccacctt
cacctgacgg cgttgatgat 1080tttttgcaac tgttgggaga aggcttacgt
agattgcccg ctggaaacag aactaagtta 1140cagtttaaat ttgtggaatt
acttaaagat gaagaatcgc gtatatcaga ttaacttttt 1200aatatgtc
12081742297DNADiabrotica virgifera 174gggacctcta aagcagtacc
atgtggataa gacattcgcc gacagaagat acaaagtaca 60gtcagcgagg acatacttct
acctcaatga agcccagtgc gagaagaaca cggaaacatt 120catcaaatgc
ttggaaacca tagcagagtt tcagcaaaca tctcaaggct ctacttatgg
180caccgggatc gcagctgtta aattcactgc tttgggcaga ccacaactgt
tgcttcaact 240ttctgaagta attatgagag caagacagta catgaccgaa
attgttggag gtgaaggtaa 300cgttttgtgt catcacatga agtatgaaga
tttggaggcg aaattcgaaa aaattcaaga 360taaagaatcc ttacgaaaat
tcttgaagca agttacatac gataaacaag gtgttctcca 420cttattcccc
tggtcgggta ttattgacga gaaccaagag ttgagtgctt ccttcagagt
480tccagactta aaatcaggaa ggatgatcag gctattaaca cagctatcac
caaaggagga 540ggagatgttc agaaatatgg ttaggagatt taataccatt
gtgaaagcag cacaggaaat 600ggatgttagg ataatggttg atgcagaaca
aacttacttc caaccagcaa ttacccgaat 660tactctggaa tatatgagaa
agtacaacaa acaaaaagcg atagtattta atacttacca 720atgttatttg
aaaaatgctt ttgatgaagt ttcgactgat ttggaacaag caaaacgtca
780agacttctat tttggagcta aactcgttcg gggagcatat ctcgaacagg
aacgagccag 840ggcagccgcg atgggctatc ctgacccaac caatccagat
tacgaagcta cgtctgagat 900gtatcataaa actctttcag aatgtttgag
gaggataaag gccttcaaac aaaaaggaga 960agacaagaaa attgctataa
tggtagcttc tcacaatgaa gataccgtgc gatttgctat 1020taaaaaaatg
gaggaatttg ggatagaacc caaagataaa gtgatatgtt ttggacaatt
1080gtttgctatg tgtgattata ttactttccc attaggtcaa tctggatatt
catcttataa 1140atacatcccc tatggacctg taaaagaagt attaccgtat
ttatctcgaa gagctcacga 1200aaataaagga gtattaaaga aaattaagaa
agaaaagaaa ttacttgcaa gcgaaatttt 1260caggcgctta tctagaggtc
aattatggta tacacctaaa ggaaattatg ttcccgtata 1320aacactagat
ctcaaacaca gcaaccataa aaccactgtg caggttttac ctagttttgc
1380gacttaccta cccgttttgt agataactag ttggcagaca aataaaacta
acgtcgttac 1440agaaaaatct gcgtgacaaa ttccaaaaat ttaacatcaa
ttggcctaaa tatacataaa 1500ataagcagtc cctatatttt tacaaacatg
taaatataaa gcaaataaag gtcattttta 1560atactgtctg ataacggaaa
gaaaagactg tgtttgataa tattttgaaa tgtcattgcc 1620ttaaaatata
taacgaagga atgttttaag tcatgaattc aaatcaaaac attttactaa
1680aacagatcat tgataaatga tctgttttta gggtatctta tgattgcata
ttgaagcgaa 1740cctcgtttgt tgatttgctg atttttattt ctaaatcgaa
tttatagtta tagaaataaa 1800aataatttta gtttatatct tattgttaac
atatttttta ggattgtgat ttttgtgttt 1860actgatgatt attacagaga
aaggacttaa acgtcgcaca tgtttttaac tgcgttaatt 1920atttgtttac
atgtatattt accgttgtga agtaggtatt tttttatttt gactaatgca
1980aaaaacacat ttcagtatag atgcgtgttg gtaacattac tgttaattac
ttcttaaagt 2040taaaatttga tttggtttcc agtaacagga cttaaagagg
taccaacaag gtatgaaaaa 2100aagcaaaagt aaattattaa accttttaca
tgccactata cgtgtagtcc agtcgggtta 2160gacgaataac tgacctaacc
ttgcatgccg agctgtccca gatttggttt ttctgatctt 2220tgggggaggc
cagtagtggt gtgaatttgg aatctcgact gaatgccgcc gttgcgttgg
2280ctcttatttt gatttcg 22971753120DNADiabrotica virgifera
175atgaatgatc cctcaaagaa agtaaaaaat ccgttgttta acatatcggt
aactggagga 60acctttgaag attttactca acatgctata ttattacccg caaaagaaaa
acaaccacct 120catacactaa atatatccca tgaacaaatt ttaaatgata
taatctccgg atcttcaaaa 180tcaagtgaag ttgtttctaa tcaagagata
gaaacagttg tggaaaatca acagcatctt 240cccgaaggaa ttaatcagtc
agaatttact tattcacaac aactgccaga tctgtcaact 300ttgcaacctc
acttaagtgc tgccccgcaa cacagttcgc acttgtcttc ggtattttca
360tctttttcta gtatcttgaa tattggaggt tctaataata atcctaaaga
agaagtgata 420gtatctccac ctgcaggaat agaaagtctg attagccaaa
gtaacaacaa ccctgagcca 480gtgccgcttt tttcggccag tttacctgaa
tatagtatcc aacagcctcc tccgcctaca 540ggtccaacaa atttattcag
aagagctggt ctaaaaaaac cggcttatgc acaagtacca 600ggtttatctt
cagcccccat agcaccccaa aatttgaatg tccaaaatat tcctccacca
660aaccttcccc cattacctcc agcagcgcca gtaccatcag cagcatcaca
aaacatatta 720gtaccaacag ccccttctcc atccacttcc atttcatcaa
cttctagtgt agaccatata 780aaccaaggat taccaaatca gtttaaacca
atatctccgc ctccttctaa tttgcaatcc 840ttccatccta tcacccaacc
tgtagctcct gtgccggtac aaccttttgc ccaatctgta 900gcgataacgc
atccatacag aaatatagta ggcccagtta tggatgccat accagctagc
960gaactatcac aaaatgctaa tttcagtagt tccctcgtga attctccaga
gtctctgcca 1020aaaaataata acacaggtga tgctagtccc cctgttaacc
aagtgtatag acctgtctac 1080catcactggt ttcatcagag agaaatagag
ggaaggaaat tatgggaacc gttttctatg 1140ttggattcat tagctttaga
acaagctttc acttcaaatg atttagatcc agataaagtt 1200attgctactg
acggcggtcg ttatgatgtg aatattttaa gaagacaaag gaatcctgtg
1260tattggaaaa cgaatcccaa tgaagtacga agatgttctt ggttccacaa
agccagttca 1320gacggaaggt tggtcccata tgaagaaaat atcgctacaa
agttagaaga agaatacaaa 1380tctgcgtttg aatctaataa gtggcacaaa
acaatcgaat tacctcaagg agaaagtatt 1440gtctttcatg gacctgatgt
tttagtactt ttctctccca cgcaggttcc tgacgcatgg 1500ggcaacacaa
caaaccagtc ccgaccgaga gttgtaaaaa ggggtatgga tgaatttgat
1560atagatgaag gagaacctgc tcaagtcgat cacatcttgt tcatggtaca
tgggattggt 1620tcagtctgtg atttaaaatt tagaacagta gaggaggtag
ttgatgaatt ccgcagtatt 1680gcctttcagt tagttcagtc tcactacaga
tcgtcctgtg accaaggaac agtcaaccgt 1740gtggaaattt tacctataag
ttggcacgat aggctacatt ctgaagaaac tggaatcgat 1800gatcaattaa
aaagtattac tctggagagt atacctagac tgagaggctt tacaaatgac
1860actttgctcg atattttgtt ttatactagt cccatttact agtcaaaaaa
ttactacaac 1920tgtgggaaca gagcttaata gaatatatga cctttttaaa
caaagaaatc ctgattttaa 1980agggggtgtt tcacttggtg gtcacagttt
aggcagcctt atactcttcg acctcttgtg 2040ccaccagcac tctaaacctg
aatctgacgg agaagaaagt gatgaaattc tttctagtag 2100tccaggctcg
gtcaaaccag ctccacctca gcgaaagatg agtaaaagaa taagctatat
2160gatgggtgct atcggtacgg gacagccgga aatacactac actcgactta
atttcgaacc 2220aaagaatttc tttgctttgg gatctccaat aggtatgttc
gtgatcgtta gaggtctgga 2280cacattaggt gaacactttt cgttacccac
ttgtccagcg ttcttcaaca tttttcatcc 2340atacgatcca atagcataca
ggatagaatc tttaatcaaa ccagaatttg caaagttaaa 2400accagtattg
attccccacc ataaaggaag gaaacgaatg catttagagt taaaagaaac
2460gatgacacgt gttggagctg atttaaagca gaaagtaatc gattctatga
gaagtacttg 2520gaattccgtt taccaattgg caatgtttca caaacagtcg
ccccctagtt tggaagaaga 2580ggtgtctaag gcatttaaag atcaaatggg
gcaactggaa aacgagtcca tcccagaatc 2640tacgggaact cctgtaggga
atttgaatag tggaagaaga gttgattatg tcctgcagga 2700agcacctttt
gagttcttca atgagtacgt gtttgcctta acgagtcatg tttgttattg
2760gcaatcggag gacaccatat tgatgatgtt aaaggaaatt tatacttcta
tgggagtaac 2820ttctgacagt caaattcctc agcaaaccat gacaatagag
agaccaccat catctcctac 2880agcaacaagg tccccaaatg ttcaaattcg
tgcattccaa caacctattg gagttgatcc 2940aacaaagccc atgcaattga
actcgtctct tgaaccacca ccagtaagtg gctttgttcg 3000aaaaacatag
tatatgctct acgagtggtt gtacaagcca cttgccgaac gttatgctcc
3060atatttgttg cacagtaaca ccaactctaa aattacaaaa aaaaaaaaaa
aaaatgcgag 3120176275DNADiabrotica virgifera 176tgctcaaatt
tctcacccac aaattaagaa ctcattctat aaatgaggat aactatgcag 60ggaaggccga
tgaagaccat gattctggta cggaatcaga tgaagagatt gatgctccag
120tatcaggatc agcatcctgt tcagaacgcg tttgtaacgt atcaccggca
gacacaggat 180gttcgatgga aagtccagct cctgatcaac acgatgctca
cagctccgaa gaagagttgg 240aagttatcaa tagcaatagt aaaatgaggc gatca
275177274DNADiabrotica virgifera 177tgattccttt atcttttgta
aataaaaatt ctggccaaat tatattagca ggtgatccta 60tgcagctagg gcctgttata
ttatctaaag ttgctaccga atgtggacta gcagagtctt 120atttggaacg
aattatgagc agatttccat attgtcgtga tccagaagga tttccagaaa
180cgtctggatt tgatccaaga tttgtaacga aattattata taattacaga
gcccttccgg 240acattcttac tttgtacagt acactctttt atca
274178275DNADiabrotica virgifera 178tggcctccat gccgagcaag
actcccgtca gcgtcctcca ggagttgctg agccgtcgcg 60gcatcactcc caaatacgaa
ctggtccaaa tcgagggcgc catccacgag ccaatcttcc 120gctaccgcgt
gttccttaac aacgatctgg tggccaccgg aaccggaaga tcgaagaaag
180acgccaaaca ttcggcagcc aagaacttgc tggatctctt ggtcggaaaa
gtgactcccg 240aacaagccaa tcagaccaac ggaacgcccg gagcg
275179273DNADiabrotica virgifera 179tgaactccag tgaatcctta
aaattagcaa aatatgaact atttccactg agcgatgtac 60aactcggttg gaataactgt
gattggtctg ttggtgctgg aatggttaat atgggaaata 120catgttattt
aaactctact ctgcaagctc tctttcatgt accagctttt gttaattggt
180tgatgtcaga caaagatcat actgccatct gtcaagattc aggaggtctc
tgtataatct 240gcgcgatgcg caaaactctc caagactcac aac
273180273DNADiabrotica virgifera 180tgggtccaca tggagatgta
ccacaacaac ctaaatcggc aaacgggcaa caaaggggtg 60gaccacaaca acagcgtagg
cctcaggata ggcagcagac agcttctgtt aaagaattga 120catctcgtat
gagtgaactg caagctggtc cacttgtacc tatgaggtta agaaaccctg
180aacctggaaa agcaggtcgc aaaatacccg tagaaacgaa tcatctcagc
ctcgcgctag 240gtaaattaaa cacggcttat cactatgatg ttg
273181272DNADiabrotica virgifera 181tgagcgagaa ggaagttaat
gctaagatcg aggagttatt gagtgacatg aacctcaacg 60acgacaaaaa gaaacccctt
agagaaatgc ccatggaaca gaaacgtgca atgttgaaga 120tgcagaacaa
aaagaaagga caacacgaca cgggcagtcg attcaacgat cccgaagatt
180attacagata tctggatcaa cacataaatc cggagtactt gcagttgaac
aaatgcctga 240actgtgtgga aagcttgagg gtagccttga ct
272182275DNADiabrotica virgifera 182tgctgttttc taatgagatc
tgggcggcta caaaacggca ggcattgaaa aggacgaatt 60cctgccagga tagaattgaa
aggatccctc ctagctcgga agaacttttc gaaaatatcc 120cttggatgaa
tatcatggct gatctgactg ttaaatctgc tatagctgaa tatgagttta
180aacgtatgct gaataaggag ctgtctcatt tctccgaatc tagcaaatct
ggcaatcaaa 240tatcggaata tatctgctcc actttcctag acaaa
275183275DNADiabrotica virgifera 183tgacagtggg gcgattggaa
aaaacgttta cagcattgcc aacaatttat agtaagaatt 60gtgtttgttt tgatggcatc
tacaccaacg accaaccaat gcaccgccag actgagagtt 120ttgtttacgc
tgacgacctc ggtatcgccg taaaagagaa aacacataca caagtaaagt
180cgacaatgga agaggcttta aacgtgatgt caacttacta caaacaaaac
tcattaaagc 240taaaccctac taaaacccaa gtatgtgcat tccat
275184275DNADiabrotica virgifera 184tgtttagcac acaattagga
aattttccat ttgctaatac taggaacagc caagagacaa 60cttccaacag tttgaataat
catataatgg atgccaacaa tggagctgga gatagtaatg 120atggttccat
agataaaacc aaactaatta ttaattacat accacaatat gccactgaag
180gtgaactaac tcaaatattt tcaaccgttg gaaaactgga agatgtaaaa
attatgagag 240actttaagac tggttacagt tttggatttg gtttt
275185275DNADiabrotica virgifera 185tgagagcaag acagtacatg
accgaaattg ttggaggtga aggtaacgtt ttgtgtcatc 60acatgaagta tgaagatttg
gaggcgaaat tcgaaaaaat tcaagataaa gaatccttac 120gaaaattctt
gaagcaagtt acatacgata aacaaggtgt tctccactta ttcccctggt
180cgggtattat tgacgagaac caagagttga gtgcttcctt cagagttcca
gacttaaaat 240caggaaggat gatcaggcta ttaacacagc tatca
275186275DNADiabrotica virgifera 186tggatgccat accagctagc
gaactatcac aaaatgctaa tttcagtagt tccctcgtga 60attctccaga gtctctgcca
aaaaataata acacaggtga tgctagtccc cctgttaacc 120aagtgtatag
acctgtctac catcactggt ttcatcagag agaaatagag ggaaggaaat
180tatgggaacc gttttctatg ttggattcat tagctttaga acaagctttc
acttcaaatg 240atttagatcc agataaagtt attgctactg acggc
2751872593DNADiabrotica undecimpunctata 187acgattcgtt tggtagtaca
tagagaaatc gtttttagtt ttataacctt ttctgtgttg 60gaatattgaa caaattctaa
tgattcgtaa agtaacactt ctgctaaaaa ccaaaactga 120tagttttttt
caaaaaaagt acaatttttc tgggcttgct actgaaataa tgtgccatac
180tctaattgtg gaaaaaattg taggtatatg ttgaaagtta acaaataact
aatgcttaca 240ataatgtcga catcaagtca aaataatggt aaaaagagtt
cagccagctc tattgttaac 300accccttcca gcactcccat tactaatctt
gcaaatggta gtagtaccca aggaacagca 360catagttcac ctccacctaa
tagcaatgct ccaaagtatg gtaccctagt gcctaacaga 420atattcgtgg
gaggaatatc agctaacaca acggaaggag aattaatgca actttttagc
480aactatggta ctgttaaagc tgctaaaatt attcaggaca gggctggtgt
atcgaaagga 540tatggtttca ttacctttga gagtgaggat gatgctaaaa
gacctcttag agaagctgaa 600aacatagtgc taagagaaag aaaacttaat
atagcacctg ctattaaaaa acagcctttt 660agtcgagcat ttgatgcatc
cagtccacct gctgttgctg caaataatgc cagtcaattc 720ttttttcctc
cgggtgctgc cgtgccatat tttcagagtg gagttacata ttatactcag
780ccagcccctg ctgcacctgg agatcccact cctcaacaac ctgtttatca
acctcctcca 840atgtatccca cacaaactgg tcctccacag ccagctacat
acccttctat gatgtttccc 900gctcaaacta tatatatgcc acaacagtat
cccatgccta ttccgtacga gtacaacttc 960taccagggta acggcccatc
gctgccaact cagtatctgg ccggaggcca aagtggttcg 1020ggaagctctc
attgcacctc attaccatca tcaaacagtc cacccagacc caatggttat
1080ggtcaacagg tacctaccta taattcagga gatcccgtct actacaactt
accaatctac 1140ggagctactt tggaaggtcc gccattatac gctgatgcct
tcgatctaag cgctagtgga 1200acttatgcgg aggaaaccta cagcagcatg
attacaactc ttactcaaga aaatgtagaa 1260gcttctcttg gatcatcgat
aaattcaact catgcagctt ccgacaattt cacacaacat 1320ctggatcaat
ctctcgttga gtctgattca agtattgtga atgttggagg taaaccacca
1380ctacataaat cctcagagac aattgtttct gctctttccc aacctcagga
ggatagaaac 1440tcacatacgc ctattgtttc actactttcc atagatcatc
aacaagaaaa agactattca 1500tcaatgcaga gcggtcgtag acgtaaaatt
ctggttcaaa gcaatcaaaa taacgtgccc 1560atgtatccta caaatggata
cgtcaaccag ttcgtcggat ataatcctca acaaccaccc 1620ccacattcat
tcggtcaacc tctctttaac aacggttacg gtttttcgga atatcgtcga
1680tttaacggat acccagatat caggtcaatt aataatagta ataaaccgag
gagacgaata 1740tacgaaaata ggcggtcgaa cgatcattct agccgttcta
gtcttcgcac agactcaaac 1800tcatcggcaa gttgtgtcga cgaaaataaa
aatgaagaaa aaacttttcg aactactgtt 1860aacactcctc cacctgcccc
atactcacct atgactaatc aacaattcaa atttattaat 1920tacagtaaca
cgaaaaacat ttataatcat aagtacgcta ataacaataa ttattataat
1980agaactgcta cacaccatga ccgatttaaa cataccgaac ctctagcgaa
taaaaacaat 2040tacagttcca tttatccctt aacaaataat ttcctaacta
atagccatac ccaaagtaat 2100tctgattccc ttccctccca aaattcccag
gtacatgtga cgactacggg tcagagcttc 2160gtcccggtga ctatccaagc
tcagacgaaa cgcaacaagc gttctttgag acggagcggt 2220gccagtgctg
gaggcatcaa cgaaatcggc gccggtgacg cccctttacc aggcgaagaa
2280tgcgaagacg tctacaagaa gctggagacg cttaagttgt aacgaaaaag
tggtatattt 2340tcttgtatat ttttttctat ttatttaaga ctgtacaata
atgtaaaata ctagaaattt 2400aatgtaaact aaagaggaaa aatatataat
acatatttat taaagatgat ggagatgtaa 2460caagttttta tttattaaaa
acttattttt attcgttgtt aacaaatggg agctgatttc 2520ttcaattata
acgtgttggt atcttgtaat tgaaaaacgt caattatagt acatattatc
2580gtacaattat tag 25931882585DNALeptinotarsa decemlineata
188gccgttgaac agtagtgaaa atgtttagtt tttggatgat ttgtgtattg
agttaacaag 60aatattctaa taatacaaga atttatgaga acgtctttcg atacatctaa
cggggcttta 120cttaatcact agtgaaacca gacagttcaa tttttcccgg
aaaacaatag aaatgtgttc 180taacctaatt gttgtatgga aaaaattgta
ggtatctcct gaaagtgaac gaataaggaa 240aagctgaaaa atgtcgacgt
caagtcaaaa taacggaaga aagagttcaa acagctctat 300tgtcaacaca
ccctccagta cgcccctcac aaatgctgct aatggaaatt cgcaagttag
360cacacaaagt tctccaccac ctaataacaa tgctcctaaa tatgggactc
tagttccaaa 420ccgaatattt gttggtggaa ttacggccaa taccaccgaa
ggtgatttaa tgcagctctt 480cagtagctat ggaactgtaa aggcagcaaa
aataatacag gatagagctg gcgtttcaaa 540gggatatggt tttataacgt
ttgaaagtga agatgacgcc aagagaccat taaaggaagc 600tgataacatt
gttttcaaag aaaggaagtt gaacatagca ccagctatca aaaaacagcc
660attcagtcgg gcatttgatg catccagtcc accaaatgta gcagctggaa
accctgcaca 720tttttttttc ccacccggag ctgcgatgcc atattttcaa
gggggtgttg cctattatcc 780acaacctcca cctgctgctc ctggagaccc
aaatgcccag caacaagttt accaacctcc 840cactgtgtac cccactcagg
caggccctcc tcaaacggcc acctatcctt ctatgatgtt 900ccccgcacag
ccaatttaca tgccgcaaca gtatcctatg cccataccgt atgactacaa
960cttctaccag acaaatggag ctccgccctc cgcacagtat attgttggag
gccagggtgg 1020ccccggagga ccacaatgtg gcccaatcgc gccttccaat
agtcccccaa ggccaccttg 1080ttatagccaa cagatgccag cctataatcc
agcagatccc atgttctaca acatacccgt 1140ctatggtccc acaatggagg
gaccacctgt ttacaccgag gccttcgact tgggaaatgg 1200tggaccatac
gcagaggaca cctacaacat gggtgataac aacattacac aacaaggcat
1260agagacttca atcccgcctc ctgtctcagt acctgctgta caaacactca
cttttcctac 1320tcataacatc aatgttcctc ctcctcctat tcctacaagc
attgagttga atgtccccaa 1380tgcctcttct cggcaccaga tggatcctgt
tgttgcagaa gatgtgtcta cacctaggaa 1440ttcagacaca cctgtagttt
ctatcctttc tatcaacgaa cagcaagaaa aagactattc 1500atcaatgcag
ggtggtcgaa gggttaaaat tcccccgcca aacctgcata acaactttca
1560aattcagcac gctcaacatc caaatggtta cattaaccat ttttctggtt
acaacggtcc 1620accaccgact cagtttggaa tgtacaatgg ttattctcag
aatggtccca ttttcccgga 1680ctatcgtcga gtgaatgggt ttgacaaccg
tatcaacaaa aatagaaggc gttcatatga 1740caatcgaaaa tccaacgatc
actcaagccg ttccagtata cgcactgatt ccaactcctc 1800ggcgagttgc
gttgatgaaa acaaaaatga agacaaatct cacacgcgca cttctgtcaa
1860cacgccacct cctgcccctt attcaccgat gacccatcaa tttaaaacta
ttgcaaacag 1920caacacaagg aaccaccaaa attccttcag atattataat
aataataatt attacaaacc 1980agcatattta gaccgatcaa aaacaaatga
cttgaccact cataataaac ccagcaacaa 2040ctcctctagt aataacagca
ccttttcttg cccaaactcc ccacaatcct ccatttccca 2100ggtgcatatg
tcggcaactc agaccttcgt accgcaagct aggcgcaata agagatctgt
2160tagacgtagc ggcaccggcg gattgaacga aattggcgct ggcgacgccc
cccttcccac 2220tgaagtcggc ggtgaggtgt gcaagaaatt ggagacgctc
aagttgtagt aggtgggaga 2280aagtcattat tatgtatatt tttttctatt
tatttaagac tgtacaataa ctgtaacata 2340ttataaattt aatgtagaaa
catattataa atttaatgta gacctatgag agggatatta 2400atataatata
tatttattga aaaatgtgta gatgtgacga tttgattttg agaaaggatt
2460ttttttggtg gtacagatga cttggaaacg tcttgtcaga tatcttaatg
tgtcatttct 2520atccatgata aataattgat gacttttcat attgtgaact
ttgttcaaga cgcgtttcac 2580tgtgc 25851892154DNAPhyllotreta
cruciferae 189gtacgatccg ttttcgtagg aactagtgaa attggttttt
agtttttcgt ccttaatagt 60gttgaatttt tgaatgcatt ctaatgattc gtaaactaac
gctgcattta aacgacgtat 120attttgaaag cgaacgaata acgaatgccc
tcaaaaatgt cgacctcaag tcaaaataat 180ggtaaaaaga gttcgaacag
ctctattgtc aacacaccat ccagcactcc tgtcacgaat 240atcgccaacg
gtaccaccca agtgagcacg cagagctcgc cgccgcccaa caacaatgct
300cccaaatatg gtactttggt gccgaacagg atcttcgtgg gcggcatatc
ggccaacacg 360accgaaggcg agctgatgca attgttcagc gcctacggca
cggtgaaggc ggccaagata 420atccaggaca gggcgggcgt atcgaagggc
tacggtttca tcacgttcga gagcgaggac 480gacgcgaagc gacccctacg
ggaggccgag aacatcgtgt tgcgcgagcg gaagctcaac 540atcgccccgg
ccatcaagaa acagccgttc agcagagcct ttgacgcgtc cagtccgccg
600acggtggcgg cgggcaatcc cacgcagttc ttcttcccgc cgggagcggc
ggtgccgtac 660ttccagggag gcgtagctta ctatccccag ccggctccgg
ccgcccccgg ggacccgtcg 720acgcaacagc cggtgtatca acctcctcca
atgtacccga ctcaaacagg cccaccgcaa 780acggccgcct actcgcccat
gatgttccca caaactattt atatgccgca acagtatccg 840atgcccatgc
cgtacgatta caacttctac cagggcaacg gagcctcgcc ctctgcccaa
900tacatggcgg gagcggcgca aagcggatcg gcggcgggac cgccgcaatg
ctcgacgatg 960ccgccttcga atagtccgcc cagaccgcct tgttacggcc
aacagatgcc tgcctacaat 1020ccgggggatc ctatgtttgc ttataatttg
cctatatacg gttcaacgtt ggaaagcttg 1080ccaatctaca ctgaagcttt
tgatctcggc gcgagcggtt catacgctga ggaaacaatc 1140tacaacaatt
tggatcgaac ggtaaccccg tcgcacgacg acgacccgac caacatacag
1200acggtttcct actgcaacac gacaaacaac acgaacggcg gcaccgccgc
ccccgaccca 1260gcacaacaac aacaccccgc gttcgtcaaa gacgagagaa
acaacgcgcg cacgcccgtc 1320gtttctctcc tctcgatcga ccaccaacag
gagaaggact actcgtcgat gcagagcggc 1380cgccgccgca aaccgccacc
cccgccgcag ccacagccgc cgccgccgca gctacacaac 1440caccacaaca
acccgaccta tcaccaccac catcacggcg cgttcgtctc gcattcgcat
1500ccgttcgtcg gcttcaatcc gcaacagccg cctccgttgc cgccgccgcc
cgccaacggc 1560tacgcgccgc caccgcaggc tatttacaac gactatcaca
ggcgcgttaa cggttgtttt 1620gttagtaata gcgtgaagaa tgtaaataat
aacggtaaaa atcgcaacga ggacggtaag 1680agtaccgtac gaacggcggt
gaatacgccg cctcccgccc cttactcgcc gatggcgaat 1740caccaattca
aagtttacac gaacagtagc gttaagaatt ttatgaacaa taataacagc
1800aacaagaaca ataattacgc tagaagcaac aaaaagtacg ctgtaatggg
cgtgccgcct 1860ccaccgccgc caccgcccgc ctctaacgca tccactacga
ccccaacaac atccaccaac 1920gacaaaatct actgtaacaa caacaataat
aacataacta gtagtagtag tagtaatagc 1980gccgccttgc ctacgcctgc
ttcctataat tcgaatacta ataataataa tagtagccgc 2040ctccttcact
ataataataa ccattcccct tcgattaacc acgtggattt gggggtgacg
2100caggtgcacc tgtcgacgac gacgtcgcag agcttcgggc cgcagacggc gcgg
21541902036DNAPhyllotreta striolata 190ttgaaattgt ttttgaactt
ttgctgtttt gtattcgttc tattttcatt ttaaacggtt 60taaaatgctt ctctgagtat
tttaaaataa gcaactaaaa acaaattcga atttttaaca 120attttggaat
cgactaaacg taaggtatat tttgaaagcg aacgaataac gaatgccctc
180aaaaatgtcg acctccagtc aaaataatgg taaaaagagt tcgaacagct
ccattgtcaa 240cacaccatcc agcactcctg tcacgattgt cgccaatggt
actacccaag tgaatacgca 300gagctctccg ccgcccaaca acaacgctcc
caaatatggt actttggtgc cgaacaggat 360ctttgtgggc ggcatttcgg
ccaatacgac cgaaggtgag ctgatgcaat tgttcagcgc 420ctacggtacg
gtgaaggcgg ccaagataat ccaagacagg gcgggcgtat cgaagggcta
480cggtttcata acgttcgaga gcgaggacga cgcgaagcgg ccgctgcgcg
aggccgagaa 540catcgtgttg cgcgaacgca agctcaacat cgcaccggcc
atcaaaaaac agccgtttag 600caggaccttc gacgcgtcca gcccgccgac
ggtggcggcc ggtaatccga cgcagttcta 660cttcccgccg ggggcggcgg
tgccgtactt ccagggaggc gtcgcttact atccgcaacc 720ggcccaggcc
gcgcccgggg acccgtccac gcaacaaccg gtctatcaac cgcctccgat
780gtacccgacg cagacggggc cgcctcaaac ggccgcctac acgcccatga
tgttcccgca 840gatttatatg ccgcaacagt atccgatgcc catgccgtac
gattacaact tctatcaggg 900caacggagcc tcgccctctg cccaatacat
ggcgggagcg gcgcaaagcg gatcggcggc 960gggaccgccg caatgctcga
cgatgccccc atcgaatagt ccgccgagac cgccttgtta 1020cggccaacag
atgcctgcct acaatccggg ggatcctatg ttctataatt tgcctatata
1080cggatctacg ttggaaagtt tgcctatcta cactgaagct ttcgatctcg
gcgctggtgg 1140ttcgtacgcg gaggaaacaa tctacaacag tttggatcga
acggtaacgc cgtcgcacga 1200cgacgaatcg accaatttgc agacggtttc
ctactgcaat acgacaaaca acaacaacaa 1260caacacaaac ggcggcgccg
ccaccgccga acaacacgcg tcgtacgtca aggaggagag 1320gaacaacgcg
cgcacgcccg tcgtttctct tctctcgatc gaccaccaac aggagaagga
1380ctattcgtcg atgcagagcg gccgccggcg caaaccgccg ccgccgcaac
cgccgccgcc 1440tccgccgccg caattacaca acaacacgac cgcctatcat
catcaccatc acggcttcct 1500gccgcattcg catccgttcg tcggtttcaa
tccgcaacaa ccgccgccgt tgccgccgtc 1560gaacggctac gcgccgccgc
aaccgccgcc gccgcctatc tttaacgatt accatcggcg 1620cgtcaacgtc
aacggttgct tcgtcagcaa caacaacaat cacattagcg gcggcaagat
1680tgttaacaac gcgaaaaatc atcgcaacga cgatggtaaa agtaccgtac
gaacggcggt 1740gaatacgccg ccgcccgccc cctactcacc catgacgaat
caccaattta aagtttacac 1800gaacagtaac gttaaaaact ttatgaacaa
taacaacaat agcaaacaga acaataacta 1860cgctagaagc aacaaaaaat
acggatcggt gggtgttcct acgccgccac ctcccgtttc 1920taacaccgcc
tcgtccacca ccaccaccac caccgccgcc acctccacat ccacatccac
1980atttgtaacc aacgacaaaa cctattgcaa taacaacaac aatagtacta gtagta
20361914893DNAVibidia duodecimguttata 191gtcgtcgcca ttttcgatgg
ttccgttttg tagtgcaaac ttcgttagtt tttgatagtt 60ttgtgtgtta ttttatcaaa
attcactaat gaatcttatt aaactatcgc taaaatcctt 120tgaagtgttc
ctttgcaggt agtactcgag agtggaagag aaatttaaag cttctaaaga
180tgtcgatacc taatcagcct aacggtagaa agagttccaa tagttctgtc
gcaaacactc 240ctgccagcac ccccgtagcg aattcgaatg ggtctgtcca
aacggtcgtg cacaactcgc 300ctccacccgc caacaacgct ccgaaatatg
gtaccctggt gccgaacaga atattcgtag 360gcggcatatc ggccaacacg
accgagggcg agctgctgca gctcttcagc tcctacggca 420cggtaaaggc
ggccaagata atacaggaca gggccggcgt gtcgaaaggc tacggtttca
480tcaccttcga gagcgaagac gacgcccagc gcccgctcag ggaggccgat
aacatcgtgt 540tgagggagcg caaattgaac atagcgccgg ccgttaagaa
gcaaatacct tccccgcagc 600ccttcagttt caacagagcc ttcgacggtt
cgggatcgac gacagtggcg gtcggtagtc 660cccagtactt cctgcccccg
acgatgcctt atttccaggg cggcgtgagt tactacccac 720cgcccccgaa
cggtccggtg cccggcgatc cgaacgccca gcagccggtc taccagcctc
780cagccgttta ttcggcacag gccggcccgc cgcagcccgc cagttatccc
tcgatgatgt 840tccccgcgca gccgatctac gtgccgcagc agtatccgat
ggccttgccg tatgaattca 900gctattatcc gaacgccgga gcccccccgg
cacagtacgt ggtcggtacc caaggcggtc 960ctccgccgcc gcagccacca
ccaccaccat cagcacaagg tcccccgctg cctccgtccg 1020gtagtccccc
tcgtccgcca ccctgctaca accaacagtt gcccttcggc gccgagccca
1080ttttctacaa cgtgcccatg tacggcggac ccatggatca tccgcccgtc
tacgccgaac 1140ccctcgaagg cgcaccatct aattacgccg aggaacaatt
cacaatcgtg cctcgagccg 1200gcgcatacga ggtagacgcc atgtacaacg
gccgtcccgg ctccggcttt aacgaatcga 1260cacccgatcc atctttatcg
cacaacgtgc cgccgtgtcg gtacagcaac aatacgacgc 1320cttacgctga
tcaacagcaa cagcagcagc tgcaacaaca acaacagagg gattcggagg
1380cggacaacct aagacagtcg aacacaccgg tcgtttcgtt gttgtccatc
gagagcacca 1440tggagaagga cgtgaatgcc atgcagtgcg gaaggaggag
gagaacgtat gcctcctgca 1500gtcccggtcc gtttttcggg ccgctaatgg
gcggcatgcc gcccatcatg ttcccgcctc 1560cgaattatcc cccgcccccc
atcaactatc atccgcacgt tcccttcccg caatttccca 1620accatcggcc
gttcaacggt tacgatagcg ctcggccgaa gctctacaac gccaacggct
1680actcgaagaa gcagcagaag aaattcaaca ataattatag accggtggac
aactcgaaca 1740ggtcgagcct gaggacggag tccagttcat cggccgccag
ctacgcggaa gacgcacgcc 1800acgcccacca gcatcgcacc aatgtgacat
cgcccccgcc cgccccctat tcgccgctaa 1860cgaaccacgg tttcacgtcg
ccgatcaacg gcatctcgaa tccgcccaac gttccgtaca 1920acaacgttcc
tcatccgcga cctagaaact attacaacaa caataataat aataacaacc
1980acgcgcatca cgataagaaa agaaataata acaataaatc tcgaacgtcg
aatggtaacg 2040gttaccacaa taataagaac aataatcaaa ggggcgagac
cagcccagtg aatcagactc 2100atgacacgaa tacatcgcag gtccataacg
gcgggagcgc aaccggcttc gcggcccccc 2160tgcccagtcg caggaacagg
aagaacgtgc gcagaagcaa cgtcgcggcc acagtggcag 2220cagcagcagc
cggcgccgcc ttgagcgaga tcggcgccgg agacgcgccc cagcagtcgc
2280aagaaccggt gtcggaagcc tgcaagaaat tggacagcct caagttgtag
aaaggtaaaa 2340aggggatgcg tgtgttaatt tttttttctt ctttttaatc
gatgccatcg ccattgacat 2400gaaaaaacca caacaaacga ttatcttcga
ataagactgt aaattttata tataacaaga 2460aattacttga tattacgtaa
gaaagggaaa tattaaatat ttattgtaaa ttcaaagtat 2520tatttttttt
gttttgtata ttttaaatta ttatttagaa aaaaaatgaa gcaagttgtt
2580caccgtagga catcactttg tatataacga taataatgat gcaaaaacga
aaaaaatctc 2640tagctatggt atatgcaata tttatttaga gtataaacga
caatattaat tgtatatgta 2700atacaaaaat taaacgagat gccatattgg
actagatatt aaaatgaaat cggtaacgtg 2760ctgaaaaaaa aagtagactc
gcgtttctcg gtttagatcg aaatcgtgcc gcgccgcatc 2820agacaaaaat
tgttatttaa tatctaagga tcgtgcgtcg ctctgttttc gttttctttt
2880tgtttctttt tttttttact tcaaacgcgt cgggtcctct tgcgctcatt
tcgtatacaa 2940ttatgttttg acgtttctcg acgttagcaa tttctcaatg
aaaaaaaaaa gatctctgaa 3000gcgaccagtt gtcaattcta ctcggtgatt
aaaaaaaaaa ttaaatttaa aacgaaaaaa 3060attcggttcg tctagacgaa
aggagaagtt gtgtgcgaaa caccgttctg ccttattttc 3120ctcttctctc
ggtggttaga ttaggttttg atagaaaata ttcgttttgt ttattttttt
3180tttgtaattg aattttttca cggtagtttg acttgtatat gcataacaaa
aaggtgccgt 3240gatctgctcc atcgcgtttt taacctcaaa cgtcatatct
tctgtcaaaa cctccgtcta 3300cgccgtcttg cgaattatcg aaatttttct
tgtcaagtct tattcgaatc tcccccctcg 3360gacggaacaa tggagcatcg
tccgcttttt gttgtgcgta cttaacgaga ttttgctcaa 3420aacggctgcc
atcgtctttc gtacgttgtt ttattgaggg tgcgcgatcg agggactcgc
3480gacatccacc cgatacgagg agggtagttt tttttctttg ttccctcgat
tttccgagcc 3540gtctgcaagt ttccatcatc gaacgtcgat gagttctctc
tttctctctc tctcccccct 3600ccagttaccg gtacgttcga agttttgtgt
cattttgaat tttaggctgt gacataaagt 3660tatttattgt tgaatttggt
agatttttta aggaagagaa tctgtgtttt ttgtagctat 3720ttattcgata
agtttcgacg aaatcggttg atctcttgga acccgactcg gctttgcaaa
3780ttttttccgt tttgacaatc gtcgttgcgc ggcctcacct tgacgactcg
acgtcgcggg 3840ttccaagact gcgcgcaggc tcgaaacgtt tttaggatca
tccggggctc gtttggatta 3900tttttcgtag attttttttt cgcgctcgaa
cgccgagata tttcgtagat ggacgaattc 3960gcccccccca aacccccttc
gaaccccccc aaaaattcga tgcgccagtc ggatcggatg 4020tttcaaatgg
ccctaagcga tcgagaaata caaaaaattg atgtgaaatt ttggtttccc
4080caaagaaata tgtaaacttt aggctcatca attgatattt atccaaatta
gatgtgtaag 4140ttagtattag taacttagtt gtagttttag gataaatgaa
ctatctctta ttcaaataga 4200tgataagacg gcaattgtct ccgttcatca
ttaaaacata caggattttg ctcaaagaac 4260ttttttcgtt ttcttcttaa
aaatgtgata aaaaaaaacg tgaggcaacg agaggttctt 4320gtttttttct
ctctatctct ctcgttcgta gggaaatgct tcctcatgtt gcaaagattt
4380cactgagctg tgtaaatacg ttcattttgt tatattgttc catttttttc
tctccaaaat 4440caaagggcag ggtcggttgt tgttgctgtt gaaattcctt
aattttttct tcaactccct 4500cgaaatcttc aatctgacga aaatttattg
aaaaatatga tatttccacg tttccattga 4560atttatcgat gtggtattaa
tttacttctt tcttttttcg agattattgt gtgaaaatta 4620aattttcttt
ctatgatgtg tggagttgaa gaaaaacggt cttttgtcat ctaaccgatt
4680tgtgtgaatt tcgtgtccgg ccctacgtcc atttttttcc aagaatatct
caagattttt 4740cgcagcagta cggtcttcaa accttgaacc cttcattttc
ctctctcccc aaaccctcgc 4800ctcgattctc tttctctcac acacacacac
acattgaacc gatttgaaat agtacctgca 4860aaatattttg taaatattct
gtttgtaaat tac 48931922051DNAOrius insidiosus 192tttttttatg
ttactttgtc ctttaatatc atactcttaa aaatcaaata atcctttaaa
60tgtcttaatt cgtctattat tttttctttc ccaccatttt ctttatcata aatttttttt
120tggaatccat ccgaaaggtg aacccaacgc ccgccattgt tcgcactacc
cccatttttc 180gggccgactt ataaaattcc gtcagaaaat attaattttg
tcaaaaaacg agaatctatt 240tagaacatga gaaactcttt tcctttcgga
actcgcgtta tactttaaaa aatttaaata 300tcaaataagg atggcggttc
aaaccaacag agggatgacg agcgaaaatg aaatgaaaga 360tcaagacgtg
gcgccggtct ttaacatcaa cgcacccaaa tatggaacag tcgtgccaaa
420cagagttttt gtcggtggca taacatctgc gacgactgaa gacgatctca
atcagctttt 480ctccaactac gggaaagtgg tcaacgcgaa aataatcgtc
gaccgtgccg gcgtctctcg 540aggttacggc ttcgtcacgt ttgaatccga
cgaagaagca aggcgccttc agaaagatgc 600tgataacatt gtacttaaag
aacgaaaact aaatatcgct cctgccatta agaaagtcca 660gcctgtctac
acaagaggtt atgacccttc aagcggacct ggaagccctt ctcaatattc
720aagctacatt caaagacaaa acggagtcgt cagctcatac tacatgaaca
gtctaacgta 780ctttcctcaa caggcagccc aacaatctgc accttcgatg
accccgttac cttccaacca 840tgtagaacct tccgtttatc ccggaaatta
cccagcgatg cctcagtgta attcgcctcc 900aagttatcaa tatggatatc
ctcagcaagc gccgatcttc attcctcatt atccgtttgc 960tatccctcag
tatgatgggt gttatcaagg agaggtacaa aactgttatg ctcaacctgg
1020aatggaatct aataatcagg tcgcgtatcc gcaatatttt gtatcgcagc
aacacccaga 1080aataacgtat tatcaacagc cggtacctca ggagttggtt
tatacacaaa gaacttatga 1140ttcgtcgact gtatatttta ctgagcctaa
gtttgacgat tcgtgtgaac catcaccgac 1200atcgccaaat caacaatcac
ctgggccacc aatgttacca cctgcaccct cgttgccgcc 1260gatttccgaa
atcgataagc caaaagcgag aaacgacgaa acgccagtcg tttcgctttc
1320gaaagtatac cacgaccaaa acgacgacta ccagcccaat ccgagatata
aaccccatca 1380aggtttcaaa cggaaacctc ccccgaggca cgaaaacaac
aacaacaatc cggatcttac 1440ggataaaaat aggatgaatc gatatgataa
aagtaggaac gttggtcaaa aacctgtccg 1500tcctatggct tcgcagaatc
gtagacccat ccacaatcca cgtcccacca acctctttca 1560atatcgtaat
caatctatgc aggtaccgca gtttccttca tactacgcct caaggccata
1620tgtgaaccaa tcgcagactt actacaacca gcgaaaacca tttcaacgcg
gcaaattcag 1680tgcaaaggcg aaaaaataca gagacgagac agaacctgaa
ggtgcagaag atgaagacgt 1740ctgcgaaatc gatcagttaa ctcctcccat
aacacccatt tcgccgaaac aacatgacat 1800atcagaatcc gatcgtaaag
acgaattaat ccgatctttt gaaaacttga aaatgtaaaa 1860agcttatttt
gaacttcaaa attttctaaa aatataaaaa gtgtcgaatt cgggtcacat
1920taaaaatttt tgatatattt gtaaattttt ttctttttca gaaaaaatgt
gccaaaacta 1980aggtttgtga taaataagcc ataatgtaac tttgaggcct
taaaattgtt tatacctcac 2040ttcttttttt t 20511932837DNALygus hesperus
193ccgctgttag ttgggtggta gttatatttt ttgggggtgt tacctatttc
ttcccttttg 60ttttactcta agttggtggt aatttggatc cgaatttgtt ttatttggtc
gttgaagaga 120gtagtaaact tgtagattag tttttcaacc ccagccccca
gttttaactc cctagtgcag 180ctcgtcgtgc tgccgccatt ctgcttggac
tgggtttttc gctgatttgc ttatctcgta 240atatggacgt aatccggtgc
cacctgatcg gaaatagttt ctaagaagaa cgtgttttgt 300atcgagctcc
tgcgttgtta attcaatcaa ctagtatcat ggagaaccct tctgaaaagg
360atggaaagga ttcgaatgag tctcaagtgt cttctccggt gagcactgtc
atcaatgctc 420ctaaattcgg gtacgtcatc cccaatcgcg tattcgttgg
agggatttcg tcgcagacaa 480cggaaaacga tctggcccag cttttttcca
gctacggaaa cgtagttaac actaaaatca 540tagctgacag ggccggtgtc
tcaaagggat atggttttgt gactttcgaa actgaagaag 600aagccaaaag
actgcaagca gatgctgagt ctatagttct ccgagagaga agacttaaca
660tcgcccctgc tattagaaaa cagaactacc aaccgagaac ttatgacggc
gtccctggaa 720gcccagtttc tatctcacct ggtcagcttt acagacagac
tggtgtaccg ttcttccaaa 780atggaatggc tttttatact gcagctcccg
ctccccagcc tcctcagcca atgacacccg 840tgcagagtcc agtggaagct
cctcaagtgt atccacctgg tgcttacggt ggtgtccccc 900aaaattcaac
tgcacaaaac ttcacttttt cctaccctgc tcaaagtcac gtctattacc
960cagcccatta cccttatcag cttgctttgg aaggataccc tgtcactgat
gtcaacaatg 1020tgctcatacc cgccggagga ggtgctgaaa atggaggggt
tgtctttgct gcttcaccct 1080acatattcac tcaacagcct ggaacccaac
aaaacgcctt ttttgatcct caaaacattg 1140cacaaattgc tccgactcaa
gaacaaatct tctatcctgc atctagaggc tacgagacgc 1200ctacttttct
atacactgaa caaagcgggc gtggaaagaa ccccaaagga accgggtggt
1260acaaggacag taacgagtca gtgactgaag aagggaatgt gctggccaac
gccctcaccc 1320ctccgatcac ccccagatct ccccacgacg cctcggagga
cctctccgcg tcccttcaag 1380gtctcaacat ttagtcatcc agatttcgtg
tcatttttaa ggcatatctt ccctctaaag 1440acttgaatat tctaatacta
atttcgttct agcacagtat ctttttgata gcaaataaaa 1500atgattaaat
ctctaagtgg ttgttggtaa tacggaagct acgaaatgga aggaacacat
1560tgattagctt tcccacttca ctaaaaaaaa tttaaaaaaa cctcgggtgc
cttattcatc 1620ttaaaatgac tgacccaagt gccatacaaa ctctttctca
tctaaatttc ctaacttttg 1680ctaaaatttc aattttgatc taggaacgct
aggaagctca acaccaaaag taaacttgac 1740tgccctaatt attaactaaa
accaccaata taaagtacaa tgacttcctc tggatacgac 1800ggttaagact
cgtcttgcct tgtccattta ccatgtaaat ttattttcga ataacttgag
1860tatttttcta gaacgcttta acagtaataa atcttttata aatatagatt
tatttctact 1920ctaaggatag aattaccttt ttgtacctgt agacttgtta
cagttataag taataataaa 1980ttttataaaa gacaaagtca ttgttgaata
gttcgtaaaa ctctttagta agttttctag 2040ttgattgcgt tgaagcactt
taaaactcgt ttttcttaag ttattgactc tatgtgattt 2100tgctcttaag
aaatacttac cgaccccttt gtgtttttcc tctcaaaatt ctataggcgg
2160tctctgtagt ttttttcgtt aacaatgaac aacctaaaat ttgcatgtct
ctgtcgaggc 2220attccttcga aaaatctttg aaactcgaag attttttgtt
tctctttttc tcttgattta 2280gttcctactg attcgtgttg tataccctcc
cttgtgatat ttacttataa ataagtcggt 2340tgtgtacagt attttttgtc
attgttacaa actgaaacta gtcaatttga ctataaaatg 2400ttaagtgata
aattctcgaa tgtgactttg aattaaggta gcactcacta tgtctaccta
2460aaagatgatc ctctgtagtt atctatgaag aactagtagt taagtgattg
acgttactgc 2520attttttcta tcgtcagacg ctatttgtat ttttcttttc
acacgaaaag aaagaccatt 2580tagatgtatt tcctacttgt tttgattgca
tctcgagaga ttcctctctt tgtatcagtt 2640ctttagaacg tatcaaaacc
taaaggctga catcttctgc caatattggt aatttctcgc 2700tatgcttgat
agctaaacaa acacttcatg tattcatcct atataacgat tttgacaata
2760cgaatttatt ttggaacagc cctcaactta ttggttcatt gactattttc
aataaaagac 2820gtcagattca aaaaaaa 28371943780DNAMegacopta cribraria
194ttttgcctga cgttagagtg tgtgttgtgt tttttttatt ttctttagtc
gctgttatat 60tttctatttt tgcatttatt ttaatttttt ttgttctggc acctcttatt
taaacaataa 120cctgtatatt ttattagttg tcagttaatt ttttttgatc
tctgtaacgt ttagtttacc 180gtaatactag atattcattt gtctgccgcc
atattattta ggtcattttt tcttaacaag 240aaataagtga aaatgaatct
tgacaactat ttaaacttga aggtatttta gaattttttg 300atctgaggtg
acaaggacaa gaaccccctt cttctacttg aaagtttttt tacaagttag
360aatttttgag tacaaaaatg gtgttggaga acatcaccga agaaaacaat
agtaaagaaa 420gcctggaaac accgtccacc atccttaatc ttaatgcccc
aaagcatggg actattatac 480caaatagagt gttcgttggt gggattgctc
ctgctacaac tgaaatggat ttaatccatc 540ttttctctag ttacgggaat
gtaacaggaa caaaaataat ttcagatagg gcaggagttt 600ctaaaggtta
cgggtttgta acatttgaaa cagaggatga agctaaacgg cttcaaaaag
660atgctgaaaa catagtttta ggacaaagac gacttaatat tgccccagct
ataaagaaac 720agggtttcag tcgagggaat tttgactgct cgactaatag
tcctctccca ccaacacagc 780tatatcgcca caatggtttt acctatgcat
ttcacaacgg gatggcattt tttcagcagc 840catcgcctac agtccccaca
tcacctcatg tggagccttc tccagttttt cagactggtt 900cttcatatgg
tgcagtagca ccgagcagtc cagcagggcc agcttaccct tttccattcg
960ctcctcaagg acaaattttc tatcaaacac ctcaatacca gtaccaccta
cctcagtttg 1020atggatgtta tgatgctgga cagatgatgg taacagatgg
gagtttagtg atcccccagt 1080actatctagc acctcaacca ccaccagaac
ttgcatttta tcctcctacc cttcacaatc 1140ctgaaacaac agcaccaata
caacaggtga tttatacatc acctcgagcc tatgattcat 1200ctttaatgta
ttctacagag ccaaagtgcg gaatggagag cagcgtagga gctcacagtg
1260gcgttagtat ggctgtggtc agtaaggagg agggagaacc accaacatcc
aaccatcaga 1320gacgaccacg tgaatcttcc accccagtgg tttccctcct
caagctgtac aaagggaagg 1380atgaccattc aaaaaagaaa cagcataaca
cccatgatga gggaagaaga caatattacc 1440tcaataacaa taatcctgat
accaccaatc ggaacctaaa acaccaggaa tcctccagac 1500atggaaacct
tccctcccta ccttcttatc ctcctgtaca tactagtcct aaccttaagc
1560accctactgt acaggttccc cagtttccct tctactccag acaggtgctc
gccccgccag 1620cgaccctagc atccttctat ccacccccaa ttccgcatag
caggcctagg tccaactaca 1680ggccaagata tggtggtggg agaggtggag
gccgtggaag tggcaataag tggctaccca 1740aagaagagga aatggggcag
cctttgacac cgcccataac ccctcgttcg gagccctctg 1800gtgtcactgg
agccactact ggacctgaag atgtcgtcca gtctattcaa gctctagcaa
1860tctagtccta gacactattt gtcttaaacc ataccaaata aggaagagaa
ccttcgcctg 1920ttataataat aacaattgaa gaaaaaaaaa taacaaaaaa
aaactgaata gtcatttgtt 1980taagagaata tatatacatt tgtttatgta
tataaaatgt atatttataa ataaatttaa 2040agcataattt gatatttttc
tctatgatgt ttaaaacatt ggaattttag ttcttaaaac 2100acaacacacg
ataatttgtt agattctagt ccaggaagga ctcatcgaga tttgagactg
2160tttgatataa tttgtaatta ttataatgtg acatttcact tgttaactta
aatgcacatt 2220atttgataaa tagtgccatt tatttttact gtatggatgt
ttgtttaatt cttttcactt 2280gcttagactt tatcagactt ttctacttct
gctaaagatg agatcattct aaagatatta 2340aattgaaatg catcatttta
tctgaaatat agatcagttt taatcttcat ttgtttgtgg 2400taagctttta
cataaatgtt taaagttctg ttattacaac ctcgaaacaa aattctctag
2460atggccaaat caaaactttt gtacctgtta aacttcttta ataagttagt
ttaattttta 2520taaaagacat ggtcttcaga taggtaggtg tacttaaaac
ataagacaga gtatccagat 2580ttttgaatgc atttctggtc tttgtgataa
tttagtccaa agctgagaat gtagtaattg 2640tataaaatat tagtatatat
ttaaatatat ataaaattta atatattttg gttaatatgt 2700atgttatata
tttattgtac tttaatgtaa gtgttaatgt taagtagttt ctagatctgg
2760ttatagttaa ttttttttga aatcgctaag atatgtttaa ggttgagatt
agatacaata 2820caataaactt gcatttattt taccctttaa aaatttattc
tatattaata tcaaaattgt 2880gctctgttca tcaggatagt ttgtaattgt
tagactgtaa gacgactttt gattgattaa 2940tcaattgtaa aattttagtt
gttgctcata ttccattatt catattataa cttgataata 3000atatttttta
taggttaaat gttagatatt aaggttcatt caattagtta catgtctggt
3060aattgtcttc cttaagtttt caattttcag atttgttttt taagttccta
catttttaag 3120aaacccttta gtattcgttg cttaaaagca caagtatcct
taactatggt ttcataccta 3180cttctgaaaa gataaaattt tttttctttt
ctgtggctta ggtgtaagac aactagtcaa 3240acacaaaatt tattcataaa
aaaaagaaaa aaaggatttg ttaatgtaag attggacaag 3300attttgttat
accaataaga taaaactgtt gagatgagat ggactatttg ttcatgtatt
3360ttcgatgtat attataaaag attttgttac atcaaaatga taagtacgat
ttgagcagtt 3420agaagaatag ttatttttta aaaatataaa tacaacaaaa
atcatcagca tagcatatat 3480ttataattaa aaccactact tcaggctgtg
atttttattg taatacattg tgttagtaat 3540gatgagttta atcttaaaaa
atgtaagttt atttatgatt ataacttaat tactttccac 3600cagttaagtg
tataagttac aagaaaatat ttgtaccata ttccaaggct gatgataatc
3660gccatactgc catgaaagtg tgttgtactt atagattttt tttttataga
accacttgta 3720ttcaatttac tatttgaaaa taattgcctt atattctact
aaattctttc agataaaaaa 37801952816DNAEuschistus servus 195tggatggtgg
agatggcctg gcgttagagt gtgtgttgtg ttttttttat tttctttagt 60cgttgttata
ttttctactt atgcatttat ttttattttt gtgtgtccgc gcctcttatt
120taaactagtt tccgtagatt tgttaagttg tcacttaatt tttttaatga
tctctgtaac 180gcttagttat ctgtaataat agatgttcat ttgtctgccg
ccatattatt taggtcattt 240tttcttaacc gaaataagtg aaaatgaatt
gtaagaactg tttaaacttg aaggtatttt 300agaattttga tctgaggtgt
gacacttgtg gtgaacattt cttaataatt taaaaggcag 360aattgagtga
atctcttact gctttataat ggctgtacaa ctaaaaaatc aaaaaaattt
420agcagctttg agtgaagaaa aggatataaa taaagatcac atagagtccg
caacttcaaa 480tataagtaat aatttaaatg caccaaaaca tggaactgtt
attcctaaca gagtgtttgt 540gggtgggatt gcacctgcca caactgaatt
agatttaatc caactattct ccagttatgg 600caatgtcact gggacgaaaa
taatatctga ccgcgcagga gtttctaaag gatatggttt 660tgttactttt
gaaacagaag atgaagccaa gcgtctacaa aaggatgctg agaacattgt
720tttaggacaa cggaggttaa atatagcgcc tgccataaag aaacagagct
ttggtcgagg 780cggttatgat tgtactgcag gtagcccaat acctcctact
caagtctacc gccacaatgg 840tataacattt acttttcaca acggaatggc
attttttcca cagccttctc ctccagtccc 900tccatcatca catgtcgagc
cttcacctgt atttcaatca ggtgcatcct atggagctgt 960aactcctagc
agtcctggac ctgcatatcc ttttccatat gcacctcaag ggcagatatt
1020ttatcaagct ccacaatatc aataccatct tcctcaatat gatggatgct
atgaaggagg 1080gcaggtaatg gttactgatg ggagcctcgt tatccctcag
tactatctgg cagcagctcc 1140tcaaccaaca cctgaattgt tctatcaaac
tgctgcaatt catccacagg aggcatctcc 1200tcagtctgtg cccccgttat
tatatgcctc ccctcgggcc tatgattccg gacttgttta 1260ctccacagag
cctaaatgtg gattggagaa tggtatgagc aatcataatg gcggtggtat
1320ggctgtgata aataaggagg agggagaacc accagtcact aacaaccaca
ggcgaacacg 1380tgagtcggcc cctgtggtct ccctcctcaa gctgtacaaa
ggaaaggagg accagcctcg 1440gaggaaacaa caccatcagc aggaggaggg
gaggagacag catcatttga acaataataa 1500tccagatgct gccaacagaa
acctaaaaca ccaacacgat actaggcgtg gcactccccc 1560ctctgttcct
ccatatcctc agaaccatgc tagtcctaac cctagacacc ctcctgtgca
1620ggttccccag ttcccgtact atccccgcca ggtcctcgcc cctccaacag
ccctcacgtc 1680tttctaccca cccccgccac cgcctcctag gcctagatca
tactacaggc cgagatatgg 1740aggaggaaga ggtggtagcc gaggaggggg
gaacgccaag tggttgccga aagaagaaga 1800gatggggcag ccattgacac
cacctataac accacgttct gaggcagctg ctgttactgg 1860cactacgact
ggacctgaag atgttgtcca gtcaattcaa gcacttgcaa tctagttcag
1920cagttgcctg gccggacagt ataagacaac atatgtctat atacctcacc
gtaacagata 1980accttcatcc tacatcgtct tataacaaca ttgaagaaaa
aaaaaaccaa aaaaaactaa 2040aatagtcctt tgttttctct taaatgcaag
agaatataca taaacttgtt tataaatata 2100tacacataat atatgtaaaa
taaatttgaa aacaatattt gtttgttttc tgttatgact 2160taaatttttt
aagttaaagg atagcacaca aataatttgt taattctagt ccaggaataa
2220atgggactta ttgtgagaaa agagactgtt tgatataatt gtaaatatta
tatatgtgac 2280gttcacttgt taacttaagc acattgttat ataaatagtg
ccattttttt ttttactgta 2340tggatcttga attatttcac caattagact
ttatcagact ttttctaact tgaacgaaaa 2400cattattgct caaaattcgt
gataaaattt tatgaaagta gaattttgga attatgaagc 2460atctcattag
aacgttgtag tctcatctag ataaatcagt tttaatctcc atttgaaaaa
2520tttttgtcaa gattttcttg ataaattgag atcttaaagt ccaaaagtgt
aacagttata 2580aaatggcaac gattagcact tgtacctgtt aacgcgttgg
ggtttagtta gtttaatttt 2640tataaaagac actatcttga aaagatgtac
ttattatgtt tggaatttgt atggaagtcc 2700gagttagcag ttttttaaag
tgcatttctg gtctttgtga taatacaata tcagaagtaa 2760gttaaataaa
tgtgtatttg tgtaaaataa tataaatata tatttagtta tatata
28161962823DNANezara viridula 196ggtgcttaag cctgacgtta gagtgtgtgt
tgtgtttttt ttattttctt tagtcgttat 60attttctact tatgcattta tttttatttt
tgtgtgtccg cgcctcttgt ttaaactagt 120ttccgtattt ttgttatgtt
gtcacttaat ttttttaaag atttttgtaa cgcttagtta 180tctgtaataa
tagatgttca tttgtctgcc gccatattat ttaggtcatt ttttcttaac
240cgaaataagt gaaaatgaat tgtaagaact gtttaaactt gaaggtattt
tagaattttg 300atctgaggtg aaattgttgg taaagatact tggaaattta
taaggcagaa gtgagtgaat 360ctcttactgc ttaatatggc tgctccaata
aaaaatcaaa aaaatttggc agcattaaat 420gatgataaag aactcatcaa
caaagaacac actgatacag catcaaatat cagtagtaac 480catttaaatg
ccccaaaaca tgggactgtg attcctaaca gagtgtttgt tggtggaatt
540tcaccttcaa caaccgaatt agatttagtt catttatttt ccagctatgg
aaatgttact 600ggcactaaaa taatttctga tcgtgcagga gtttctaaag
gatacgggtt tgttacgttc 660gagaccgaag aggaagccaa acgtttacaa
aaggatgcag aaaatatcgt attaggacag 720cgacggctga atatagcacc
tgccattaag aaacagagct tcggtcgcag tacctatgat 780tgttcagcgg
gtggtccagt atctccttct caactgtacc gtcaaaatgg cgttacatat
840gcttttcaca acggtatggc tttttttcaa caaccaactc catcattacc
tccatcagcc 900catattgaac atatgccagt cttccaatca ggtccgccat
atggagcgat gacgcctagc 960agtcctggac cagcataccc atttccgtat
gcgccgcaag gacaactgtt ttatcaagct 1020ccacagtatc aatatcatgt
tcctcaatat gaaggctatt acgaagcaag ccaggtgatg 1080gttactggat
tatataattg tgattttcag caatatgaag gctgttacga agcaagccag
1140gtgatggtta ctgacggaag cctggttatt cctcagtatt acctgacaca
tcaaccaaca 1200cctgaattgg ctttttacca gccagctaca gtgcacccgc
aagaggcatc accacaatct 1260gtgccacctt tgttatatgc ttcacctagg
acttatgagt ctgctattat ctattccacg 1320gagccaaaat gtggattgga
gaatggtatg atcaatcata atggaggtgg tatggctgtg 1380ataaataagg
aggagggaga accaccagcc caaaacccga ggcgaacccg tgaggcagcc
1440cctgtggttt cctacgttaa aatgagtaaa ggaaaggagg accagcctcg
aaggaaacat 1500caccaccaac acgacgaggg gaggaggcag catcatcatt
tgaacaataa taatccagat 1560gctgctaaca ggaacttgaa acatgatgca
aggcgtggca ctcccccctc tgttccccca 1620tttccagcca accatacttg
tcctaaccca agacaccctc ctgtacaggt tcctcagttc 1680ccgtactatc
cccgccaggt cctcgcccct cctacagccc tcacgtcttt ctacccaccc
1740ccaccaccac ctgcccggcc aaggtcatac tacaggccga gatatggagg
aggaagaggt 1800gggagcagag gaggaggaaa tgccaagtgg ttgcctgttc
ctaaagaaga agagctgggg 1860ttgtcccagg cactgacacc acccgtaaca
ccccgtaatc ctgatacagc tgctgttact 1920ggcactacga atggacctga
cgacgtcgtc cagtcgatcc aaggactttc actctagtct 1980agagctgttg
cctggccgga cagtataaga cacgatatgt ctatatacct cacatcagat
2040aaccttcatt ctgcaactat caacttgatc tcattgaaga aaaaaacccc
aaaaaaaact 2100aaaatagtcc tttgttttct cttaaatgca agagaatctg
cataaacttg tttataaata 2160tatacacata atatatgtaa aataaatttg
aaaacaataa ctgcttgttt tctgttatga 2220ctttaaattt ttaagctcaa
acacaacaca ccaaatattt gtgaaatcta gtccaataca 2280ggactcattg
agaatagaga ctgtttgata taattgtaaa tattatatat atgacgttta
2340cctattaact taagcacatt gttgtaaaat agtgccattt tttttttact
gtatggatct 2400tgaattattt cactatttag actttatcag actttttcta
ccttaaacga aaacattatt 2460gctcaaaatt ggtgataaaa ttttatgaaa
gtagaatttt gggtttataa aggtttatag 2520tttctcaata gaacactgta
gtctcatata gataattcag ttttaatctc catttgaatt 2580ttttgcaatc
caaaagtgta acagttataa aatggcaagg attaacactt gtacctgtta
2640acgctattgg aattggctag ttaaattgtt ttaaaagaca ttatcttgaa
aagaaagatg 2700tacttatact gaaatttgta tgaatgagtt ggcagttgtt
taagtgcatt tctggtcttt 2760gtgataatac cagtctatta aagtatctaa
taaatgtact tatactgaaa tttgtatgaa 2820tga 28231972006DNAHelicoverpa
zea 197caactgttga cctagttccc tcgcctagta aaaatagcgg tcgacaatag
ctcaaacact 60atttatagtg cgtatctaaa aaaatctcaa atcacacact gttatctcgg
ttgttttttt 120tcgaaacatc actcgaatac cgtccgtgtg tcagtcacgt
cgtggtccgt acggcgcaag 180tctggagccc acctatacaa cacaagtgct
accgtaatcg ataaaacctc gcgtgaaaga 240cagtcagttg ttttttcgtg
aataccagtg cccttttagt tgccgctaga gattttagtt 300tagtaaacaa
gggaacatgt tatattagtg ttttaaagtg ttagaaacaa tggaaagtgt
360taagaggtac ataaggagtg agtctgcgag ttgcgctcat atggttgaag
aaagcacggc 420gaataacagg aagacggcag acccccacga gaagctgaag
gctttgttga actcccctga 480tgacgatacg gacgacacct tgcctccatc
agatgctcct aaattcggga cagttataca 540caatagaatt ttcgtgggag
gcttctcttt gaccacgact gatgaggacc tgtggaagtt 600cttctcaggg
ttcgcgacgg tcacagcagc cagggtgatc tacgacagag ctggcgtctc
660caagtgttac ggcttcgtca cattcgccag ccccagggtc gcgaggctga
tcgttaaaca 720gagcggggga gtgatgttct cgccactggg gcgactgcgg
gtggcacagg ctgtcaggaa 780acagatgagc caactcccag tgctaggctc
agaggcagcc ccgctccagg ctccactttg 840ctaccagctg ctgtgtgctc
cacccctcgc gcctctgcag ccctgcgcgc catgcgagtt 900gcctccacca
ccgtatgctg tgtacccgct gccgtttgac acaaccccag cgatctacgg
960tcccccagct cagtacgcgc tggtgccagc tccatacagc actccatgct
gtgagccctc 1020atgctgtgca cccctcgaca agcagccccg ggtgccgccg
ccaccgttac accccgcgtt 1080catttactag actcaccccc caagaaatat
attaaaggca ttctcgttta gatttggaag 1140tacttaaaaa ttactactga
tggtggattt aaggtatatt tgtgagagaa tgaactattt 1200attggaagta
aaacacgttc taataacagt atggaacata ataacaactg tatggtataa
1260agcgcattgt acatggaatc gtgagtacag atgctgtgaa ttttttaatg
gaatgtacag 1320ctgtaacaaa gtatacaaaa agccagcaaa agataccgca
agatacgaaa ctttaacaag 1380ataaggccac aacagcaatg catcttgtag
ataacgtagt atttgtcaaa atattaacat 1440tctctatatg aaataagatt
ttttccactg tcacgataat atctcctaaa aatactatca 1500ataattctta
tagccaatgt ctttgatata tttctgctca taacatcatt cgcgaagaag
1560gtttatttac aaattgttca gcttcttcga tgtattccat cttgattgct
caagtcttcc 1620aatccagttc atgctcatct tcaaacataa ctcctctatg
aataaactgt ctgtgagttt 1680gtttgtgact aatctacgga catccagctg
cgatggtgta agtgttcaag ttgtcacgtt 1740tgtttacatt tctctgattg
tctggtcgct tttatttata gacgtaagta cctatattta 1800gcttcaacct
ggtatattta agaaaccgtg gattcacgtg ctatcattat tattatagtg
1860aattttattg gtgtatcaaa ttgctattta aatcagataa ccttgatcat
tccgtgtcgt 1920gactttcaag tttagataac aataattatg tcatccacat
acctacaacg atctaaatgt 1980gacatgcttg tatctgtgtt aaattt
20061986665DNAOstrinia nubilalis 198agtgcgtatc tttaaatctg
aaagcataca gtcttatctg gttgttgttt cggcgacaga 60tgtggagtgc gtaaggccgc
tccgcgcacc catgtacacc tcgcaaaatc gatagctcag 120cgtccgtctc
agtgtcgact tgttttccat cgaatttagt gctcgcttag tccgctagcg
180tttttgttag aagtatcatg ttaaattaat aatggaatcg tcagtaaaga
ggtacattag 240gagtgattca gcgaactgcg cgcatatggt tgaggagagt
gcagcgagtt gtagaaaagt 300ggtggatccc cacgagaagt tgaaagcgct
gctgaactcg cctgaggagg acacggacga 360ctcgttgccg ccgacggatg
ccccgaagtt cgggactgtg atacataata gaatttttgt 420ggggggtttc
tcgttgacga cgactgatga agacctgtgg aagttcttct ctgggttctc
480gacggtgacg gcggcgaggg tgatctacga cagagctgga gtctccaagt
gctacgggtt 540cgtgacgttc gcgagtcccc gggtcgccag gctgatcgtg
aaacagagcg gcggagtcat 600gttctccccg ttgggacgtc tccgagtagc
acaggcggtc agaaagcaga tgacccagtt 660accagtgctg gactcgggag
cggcgccgct acaagcgccg ctgtgctacc agctgctatg 720tgcaccgccg
cttgcgcctc tgccaacctg tgcgccctgc gagctgccgc ccccgccata
780tgctgtgtac cctctgccat ttgacaccac tccagcaata tacggccccc
cagcgcagta 840cacactagtc ccggctccct actcggctcc ctgctgcgag
ccagcttgct gcgcacccct 900cgacaagcag ccccgtatcc cgccgccacc
actgcacccc gcgttcatct actaggccag 960ggccattagt ttccagaagt
gtgtcttctg aaagcctggc tctgggagct ggtaaggttt 1020ttgctttgga
atttccaggt tttgatgaaa tcggcaataa agtaaacttt tcaggaggct
1080ttgattcctt tgaagatgaa gaaatgcgac tgcaaaggat aaggttgatg
atgaaagtta 1140tgatttcgtc gatagcaaag tgaagttttc aagaggcttt
gattcgttgg acgtaaagga 1200aaagtaaccg aatgcaaagg ataggattgt
tgatgaatga attgttaagg aagtagacta 1260attaaaagca ataaattctc
aggttaagta gattggaaga agtaatgcta aggaagtaaa 1320ggacaaaatg
cctctcgaaa gttcttggaa attcaggaaa ttagcactca agccttgtaa
1380catcaatgct cccagaaccc agaacctttt ggttagttct caagtagacc
agtttcctgt 1440tcattatgcg ttttatatct atcgcataat gttccagtca
ctggctttga tatttgagaa 1500cgatgacgta cacttttgga aactaatcta
agtagatatt taagtgtgta tttaggggat 1560catcgtcaga aactagaact
ggttggtgca gtgagagcat cctcgttaat tttatcagaa 1620gacgaataaa
aatgacgtga attttaacgt aatcgatggg aaattttcga tcgtctgcaa
1680gcttcctaaa taccccaatt ccatattgtc tatcaccact cccccattgg
taattaaaca 1740tggttcctct ccttaaggtt tggtttctta ggttagtttc
atttgaaggt tgttcccaaa 1800ttgttccttt tgctcaagtc ttccaacttt
atcatgctca agttgtttca cttccattat 1860ttcaagccat tgtaatcagg
cgattttgtt gtggtattag agtaaaacta gctgtactat 1920taaattttgc
tgaatttgaa ataatgatag aggaaactta ttcgaaagaa atatcaggtg
1980atctgtattc ttcttataat gatgagccta aatcctggtg ttttatcatc
ttgctaatga 2040agtgtgaaat atttcaatga agtttatatc atcatcatca
ccaacagccc tttatagtcc 2100actgctggac tcctttagtg gaggaaagtt
acttgatgtt attcaataca aaggtgtccc 2160taatgccaca agaaaatcgc
ctgaacaaat tcctccatta ccctatttat agaatttcga 2220attgattggg
aaacgtgaaa gatctggtgt tgaaagtgtt catggtaggt ataactttct
2280ggtgtgtttt gaccgttttg tctcctgtac gtagtctggt tgcttttatt
tttagactta 2340agtgttagct tggacaatcg gtgtaaaaac tgtgggttcg
tgtgctatca gtaccatagt 2400gtgtaatata atagatatat aaaatcattt
taacttcttt acttgacgga aattttctat 2460ttgcaagaga attttgtctg
tcaccatgtg cagagttgtt cttagcaagt ccatgtacca 2520caagataatt
aatttctaaa cttgatttga aattcaaaaa tcattgaaga aattggtcat
2580tttgaagtag aattttgtgg caaaagaaaa catgaagtga ttttatgtat
ctatgaaaga 2640tgggtgttag ataataaagt gcaaaatgag cacaacgatt
catagcacaa aacggctaag 2700ttgctagatg tagaagacgt tacctccgtg
gaaaaagaca tatttgttgt attagcttgt 2760tatccttgaa aaggagtttc
gtttacctta gtgtaaggtt gttttcgaaa gcaattgaca 2820tagacagaga
tataaaccta ttttttaata aatttagttt ttacatgttg ttgattgtca
2880ctttattaaa tctggggtcc aaatgtgaag gcatgtaaac attgaaactt
atttctttta 2940gcagatgtct gtggtgtatt tgatgtttat atttctacac
atgaatgttt gttgaaaaga 3000cgacgataag aactttgttt cagatataca
atagttgttg cagtgttaaa taatcctttt 3060gacgttgtta tttgcttgtt
tggtaaataa atagatgacc aggatgttat agttctcata 3120agactaattg
caaagtcagg aacgtttatt aatatgatta gttatatcga attctattaa
3180aaatgttaca ctgtcatgtc ttccttgttt ttctgagctt catcgttatt
taccaaacaa 3240catggtttat tttatagaca gtttgatggc taccttacgt
taggtgtaat gttagttgtt 3300attacgttgt ccacaagttt tgatggatcg
aagtacacaa atcgtcaacg atgtgatatg 3360ggatattcaa taacgtagca
ttagttttat ttgttagaag acttaatagt tttagattca 3420acacattttt
aaataacgtc gtttgtctat cactcggaaa aataggacca aagacattcg
3480cagaagcgtg caaatggtca cctgtagtag tgagtcacaa agtctcttaa
tacgtaaatt 3540cgttgtaagt gggtgttgat aatttttata ttcgtcactt
ttagagctct ttaatagagt 3600ttcctgtaga cctagagagc cgatgctctt
caacatttcc aaattgtgtt gtattattaa 3660aattttatca cgagaactag
cttgctcgtc caagcaataa attcttgctc tgcccttagt 3720aataaggctt
atctcaatta ttatattaca tcatatacga ttagtattac tggaaatgcg
3780gtcaatttgt cgttttggat ttttatgact attattgtta gtactaagtc
tagtatattg 3840tgtaatcttg cgatgctttt gtgctcaagg tgttatcttt
tttttttgtt ttatttattt 3900ggtacacttt tcgcaacttg ggctatgttt
tcacagcgct tacaattaaa gctttctttt 3960cagcttttaa tgtatgctag
tacttggcta gtaagctttg ctttaggaac gacactggag 4020catttggtaa
gtgtgattga atgtacacac tcaaaaatag tcgcatctga tcttgcattg
4080atttttctct ggccagcatg agatggtatt gccgtatcct ctaaaataaa
ttttatctta 4140attatcacag tggaaatcta agtgcttggc caggttgcac
agacgggtgc atagcgtcaa 4200acatataacg cgtacgggcg ccattatcga
cactcgatta agatttcata atatgtttga 4260gtacaacaaa gcgatgcgcc
tttgtgtgca gcctggtcaa gcaccataag tagtactatt 4320ttatcgtata
gttaaatcgt cgttttatta tttcaaagca aagtgaaagc atagccatta
4380attattgtgg tcgtataact atcatgatgt tatggatgtt tcggcatgtt
ctgttgatgc 4440aaagtgttgt gaactcgtag tctcttctgt gctatgtgaa
gtacaagata ttttatatgc 4500gagctattgt aggttattat taagcatgaa
actctatcta tcacttatcg ctgcttggtc 4560tctatcatga atatgtatgt
ctatttcttt atgtggtatc aatagcaaaa tttgtaggac 4620aaacaaaact
ttttcatttt attctgtcta aactgccaag tttatattat tatcttttct
4680cttcaacttt tttctactat ttctttaaat attattcaac ttaaagtacc
tagtaatact 4740cattattgtt acaagcatta tagtagtaaa gtttccgtat
taaatggaaa aaaatatcaa 4800tttcgtgtat tgatggcatg ataagagttt
gttataggta atttatgatt ctagctacaa 4860taataataat tatgcaagat
atagaatttt taagtaatta ttttgatgtt tttttttata 4920gtattttata
tcagtaaaaa catatgtatt catggtattg gccttttcta ccaatattgc
4980gtggcctagt gacaactgcc tgacatatcg gataattaat cactatgcta
tagtatatta 5040ttttttgtat aacttgtctt gtatataatc tctcgctaga
tatattagat tcgtgaaatt 5100aaaatgatgt aatttagaca atattactct
aaactatatt aggaaaaata ctaaggtaag 5160taatatagtg taatctatgt
ctatattctg tgtaagtgga tcactcctat atcttttagc 5220aaaggcttag
aaaacgttgt gattcagaag ttttgaatat ctaaatgttg ctaaattgtg
5280taagaaatat aaaagtatat tagggaagct accctcctca gatatcgcta
gattagcagt 5340aaaattttgc agacacgtct ttaataaaat atagcttaaa
ctgtcattta tattatatta 5400tcattaataa tatatttgtt cgtactttat
attattttct agctgaaact gttaataata 5460ttcaaatttt catataggta
atatagccgt tcaggacttg aaacaaaaac atttttttaa 5520tccgtttacc
aaagtacttg aacaaaaaaa gcagcagtca caccagcaaa tgtcaccttt
5580gattatattt ttgttatttc atagaatata tctaaatccg tctgaatttc
cgctgattta 5640tttatactta tatgtatata cagggacgat gacaatatac
aaaagttgtt aagaaaaaat 5700aaactttttg agtagttggt aatcctgaga
agaatgaacg tccaatttgt ttttgtttac 5760atttcgcaaa ggcagaatgt
atgctgttta attgtaatgt tgttaccatg ttgatatcaa 5820atatcaacag
ctgtgtgtta tgtcatccgt taaaaatagc atcattatta gcaagaataa
5880taaatcataa tttaacaata ttatttagtt gattaataaa ttatattatg
gttgtcacct 5940aaaaggctta aaatgactac tttttcagta gttaattaat
ttacaaattg taaaacaaaa 6000aagcaatcga aaaaaactta acattcatgt
tgttgttcaa attctatttc taaatctcga 6060tggtataatt attaaggtgg
atacatactt tgtgaaacga acctacatta ttatttcagc 6120aaatgcttca
caatcaaaat gcctcagtgt gagctgtgca taactatgca gtagtagagt
6180agctgttcag catgctcttt ggaacatatt tacgtgaatg acatacaggt
agatagattg 6240tatttgcttg cttttcacgc aaaggataat gctggtatgc
ttacaaaagc acctttaaca 6300gaggcattcg ttttacaccg tgtacggtgt
atgcctcaaa aagtctgtat ctacctttat 6360atgcactttg cttcggcggg
tagtctacat gggagcttcc ttatttctat acaacgactt 6420gtaatataac
aaaagctttt gaaatattct aaactacgcc taccagaaat tatctattta
6480actagtcaaa ctctatttaa ttttattcaa accaagaagc tgtcacgcat
ttgtctaaag 6540cgttaccgct atttagttat gaagagttca taattattgt
tgtacttaat aatgagttgc 6600taactaatta actgcgtact taaataaaaa
tatatcaaaa taatatttat attttttgtc 6660caggt 66651996032DNASpodoptera
frugiperda 199gtcaaccgct gacctagttc cctcgcctag taaaaatagc
ggtcgacaat agctcaaaca 60ctatttatag tgcgtatcta aaaaaatctt aaatcacaca
ctgttatctc gcttgttttt 120tttttaaaca tcactcgaat accgtccgtg
tgtcagtcac gtcgtggccc gtaacgcgca 180actcccaaaa cctacctata
caacataagt gccaccgtaa tcgataaaac ctcgcgttac 240agacaatcag
ttgttttttc gtgaatacca gtgccctttt agttgccgct agagatttta
300gtttagtaaa caagggaaca tgttatatta gtgttttaaa gtgttagata
caatggaaag 360tgttaagagg tacataagga gtgagtctgc gagttgcgct
catatggttg aagaaagcac 420tgcgaataac aggaagacgg cagacccgca
cgagaagctg aaggcgctgc tgaactcccc 480cgaggatgac acggacgaca
ccctgcctcc gtcagacgcc cccaagttcg ggacagtcat 540acacaataga
atcttcgtgg gaggcttctc tctgaccacg actgacgagg acctgtggaa
600gttcttctca ggtttcgcga ccgtcacagc cgccagggtg atctacgacc
gagctggtgt 660ctccaagtgt tatggtttcg tcacattcgc cagccccaga
gtcgcgaggc tgattgtcaa 720acagagtggt ggagtgatgt tctcgccgct
gggacgactt cgggtggcgc aggctgtcag 780gaaacagatg agccagctcc
cggtgctggg tacagaagca gctcccctgc aggctccgct 840ctgctaccag
ctgctgtgcg cccctcccct ggcaccactc cagccctgcg ccccctgcga
900gctgcccccg cccccatacg ccgtctatcc cctgccgttt gacaccacac
cagcgatcta 960cggcccccca gcccagtacg ccctggtacc agctccgtac
agcaccccgt gctgcgagcc 1020ctcatgctgt gcccccctgg acaagcagcc
cagggtacca ccgccgccgc tgcaccctgc 1080attcatctac taaactcact
cactcacgaa atatctcgaa gacattcaaa ttcaaggtaa 1140ccatttcata
ctagatgtcg agtaagcgac aaaaatgtat tgtgaagcta atatagcaaa
1200aatcgaacag aacatagttg aaattagtaa aacagataga gttaatatgt
gtaccaatga 1260tgtaactagt caggtgtaag tcaaaacaat aatggcaata
attgtggaca acgaatgtaa 1320tgcactaacg taagtactga gttttgtttg
tagcactgat cgtgtacata gatgtaatgg 1380gatgacgcgc tgataacgac
ggagtaccga gcgatctaaa gataaagcga gatatcaact 1440cgaagcaaga
taagacgaga gcgatgcagc tccttacaaa tacatttcac cacgtctgtt
1500tgttctatct cgttcctctc ataaataatt catatttcaa tgtcttcgat
atatttccac 1560acgacaccat tcgcgaagaa gattacttta ctaattatat
tcagcttctt cgtatgtatt 1620ccatcttgat tgctcaagtc ttccaatcca
gttcatagtc atctgtcttc aatacattac 1680ttccctataa tgaaactatt
tgtgagagat tgtctgtgac tattcaatgg acatcaaagc 1740tgagagaagg
cgatgtatgt gttcaagtta gctctcgggt ttgtttagat ttttctgaat
1800gtctgtcttg tcgcttttat ttatagattt aagatattta gcttgaaact
ggtatattta 1860agagaccgtg gattcgtgtg ctatggttat tattatagtg
aattatatcg atgtatcaaa 1920ttgatgtttg aataaaagtc gtgcgatgtc
ccgtggaaat actttaacca caaatattta 1980atgtcatctg catgtataaa
tccaattatc ataatgtgta tattcatata tctatttaca 2040cagtcttgta
tttgaatgcg aaaagtaatt caacattttt atcaaagtta tcgctctata
2100gtaatattgt aacgcgccca agggaaatca atttgataca tctttagttt
ttctcatgat 2160aagtgtgaaa ataccgaata taaaataact ctagtgcgga
tatttctaca aaatatttaa 2220ctgaatcata tttacagaat gtatgttaga
gcagtccaca gcctcggtct aagtcattgt 2280aataatgttc acgtaatgta
tgctaatgaa attattcaaa tatgtaccgt acctagtttc 2340catatatccg
cactcgagtc aagtatttac aaacacagtt caaaaatgtg cgttataagc
2400acaacgaatt aagcacaaaa tcgaatagca gctagtttta gatgacgtta
cctctgataa 2460aagacagatt tgttattgct tgttatcctt gaaaaggatt
tttgtttatc cttagtgtaa 2520gattgttttg tgaaacccca ctgttggttg
gcggacgtct tgggggtggt gggagacacg 2580attttgatag aagacaattt
gtagaataat cataacttgt aattgagtaa gaagatattg 2640gtaagagaga
tatatcgctt gtacgagctg tagatgtgga ggtaggtaga caattagaga
2700tcttatcggt tggctgcttg cctacttaac gagggaccta gtttgaagat
aataatagta 2760cttttagagc cagattttag aaccaacctc aagacgtcag
acaacgcgtg actagaacag 2820agagaaacaa aaatattttt taatattatt
tagtttttac atgttgctgt tgaaatacac 2880tggggtccaa tatgtgataa
ggaaatgtga aattcaaagt tgaacgaaca ttttggaaat 2940cacatttcta
tacccgttat tgttgcgttg gaaataagaa tcgaatcagt ttttatgatg
3000cacacattaa cttgctaaga cgtgacgtgt ttcagtaaga tctacttaaa
gttaccggag 3060aatgttgtca aagtatttat gaggaacgaa atgaagtata
gatgttactg agacatgtta 3120acgatgcaat cacgaatagt atttgttttg
taattgttaa cattgattga acagccgctg 3180ttcgcttcga agtgatcaac
aatagacttg acaaacggat ccataaagca ataatgtcgt 3240caagatgtga
tatgggataa tttgttgact agcaatagtt ttatttagat acattagaga
3300gtagattgtt ttaaaatcct tcattttacg gcccgttgca tcgtatttaa
cataaatatt 3360gaagaattta aataattatt gtataaggtc tctagcacat
gtgataactt ccatgtcctc 3420gttcttcgtc ctatggcgta tatgtggctt
gtctaagcta gaatttcccc ctgatgcaat 3480tgggttctca cttagtacca
gattatttat aggaccaaac aaagattccc ttacatcacg 3540actaacgcag
agatcttcgc atctattctg tctagactag tatacctatc acaaatctct
3600atacgtaaaa ttgttgtatt aatattaatc attttgatat tatattctat
cactttttat 3660ggccgctagt tgtgtgatgc tacggtgaat gaatttgcgt
ttatatcaag gtcttccttg 3720ttgtttttta tatcactggt taagacaact
tcactgttat tgttattatt tattttcatg 3780tttactgtcc cattgtttgt
aggtgatggt tctttaagat caatttctag tactgtcgtg 3840ttattacctc
ataggtttga ggtaatgtct acatgattct tccactagtc agatcgacag
3900attgtcagca gagttagcag tacctgttag ataagtaaag gttccattaa
tttcttcgtc 3960caaatgttaa gttgtttcta ctctatcagc aagttcattc
acccaagcac gttcgatgtg 4020gtcttctgcc tttagatgta aggctttcgt
ctcaattact attgtacatc ataattatac 4080gattagtgtt attgaaaatg
ctgtcaattt atcgtcttgg atttttataa tgtatgtatt 4140tattgttagt
tgtaagtata gtacttatgc gatgcttcgc tcgagtatca tttatgtctg
4200tatcttcgtg tgcttataac ttaagttcct tggatttgtg ttacctcaga
ctaaagttta 4260tatctaaaat attgttgtta tatcatattc tatctatgtt
ggttcatgcg atgggagttt 4320tattttgaat ttggggaaaa tgggccatgt
ttgtggaatg tcgttattgt ccgaaacgtc 4380aaaagatact aactttaatt
caaggtcact gaaagtatga ttcgatctaa ttgaagattt 4440taatcatgat
caaggtttag agcagctgat gtgttcacac aaaatccgta acgaaatatg
4500aaaatgtctt agagatggct gttgctaggg ttgcttcaat gttggtcagc
tatgaccatt 4560ttctccaaca tcaaatcatc gcgtgaacca gattagcact
catcgtatac atttcgatgt 4620tgcactatcg atgtttcgag tacaaggaat
ctatatccaa gctaaactta agtctaataa 4680ctatcacttt cattgcttct
aaggtttctc acgtaaatac atattaagca ttcaaaaacg 4740catttattaa
gattgaaaca cccataaaaa attgtgaaaa atgtcttaaa ctgtgagttt
4800ttgttccaaa ttatgaatta tacgactatg aagtgctcat atagcaattt
tttgatgaaa 4860gaaatgagtt gttttctgct gtagatatgt atttacgtga
gaaacatact acgaacataa 4920taagtggcct agagacatac tgcctaacat
atcggataac gaatcactac gctatagtta 4980gttatttttt ttataactgt
atataatctc tcgctaggtg gtcatagttg tgtggacgaa 5040tataatgtta
gacaatatta atgtaattat aggttaggaa gattaacgtg tatttattga
5100aattgtcact tatcttagga ttggcttagt attgtgaatt gttttttata
ggactgactt 5160tagtatggga agcaatgatg gtaatgagta atttaaagga
gaggatgtgt taagaaaagg 5220taaacatcga gtgggagaaa gggatgttta
aagatttcta tttaaagtag aggttcaatt 5280tatccttgga ataaagatca
gaatgcaaaa gataactaaa aatgagatta taagaaagga 5340ccacacacat
aaatggtcat ttgacttcct aaaatcttgg agattttgaa ataggttctc
5400tggaaagcgt tagtgttctc ttaaaggata attagagtca gttctataaa
aacactatct 5460gtcgccatgt gaactgtagt tataattaat tttagtgtga
aagctatcaa tagcagtagt 5520gacactcact gtatcactgt cggtcacttt
ctcatcatcc tgattggtta ctggttcgat 5580attatctttt attactttat
cattattatc tgtaccctta gtacgaattt gctttacgtt 5640taacgagatc
gaaattttag cgcatttggt actctgattg gttggtttat tgtagccggc
5700caatcacggc gctgaacgcg gtaaagcaaa cttatactaa ggtctcagta
acccccaaaa 5760tcacttcgat gtcgtatttg tttgtgactc gatatcggat
cggaaaatct gaagacgctc 5820ctattgaatg cggtttttat tcctacatgg
aacaattaat ttggaatgtt ttcttaaatt 5880acggtacggt acctatgttt
tctcgtacaa aattcaacgc gttcggtgct ctgattggcc 5940tggttaattc
gcaacggcca atcagagcgc cgaacgcggt ctcatttcgt taatgagcac
6000aatttttata gctgttagca ctcagatttt ag 60322003624DNADiabrotica
undecimpunctata 200aagaataatc atacaacaac tattaccggc aaagtttttt
cgaataagtt gttcatggtt 60taatttgttc caggagacca ctttaattta gagcccactt
aaaatgccac ctaaaaacaa 120atctagaaat gcattttact actttatgga
agatttcaaa caaagacaag gatttacttc 180taaatccatg aaggatgtag
cagaagctgc aggtccccat tgggctagga tgtctaaagc 240agaaagaaga
ccttatgaag acagagcaaa caaagagaag ggaggtttga gatatacttg
300ggatggacaa tgcgttgaag aaatagagaa gagacaaaaa gctgaagaaa
ataatattgc 360taaaatgaaa gatgacatcg actgcacctt gaaatttgcg
aaagaaaatt gtcgcctccg 420agatgaaatg ttcttcttaa tccatatcaa
tactttctgt tatcatgcca gtggtaatcg 480gtattatccc gctgaaattg
ctatttcctg ctttagtttg gaagatggcg ttttacctca 540aaatgttttt
cataaaacta ttaagccagg tagactaccg ctggggtatg ctagtgatgc
600aaaaaagcaa tctgaagatt cacatcaact tcctatccct ttaactggtg
aatatgacga 660caatgtggaa gaagtttata cagaaatgaa agagttttta
tcctctaaaa caggaaattc 720aaaaaatttt cctcccttat atgctaagaa
agatatcgtt aaaatggttc aacacgtgtt 780agatagttgg tgcgctgatt
ttgatgagcc gccattattt aaagtttatt atttacagta 840tatgtttcaa
gtactaaaaa actcggtagc taatgacaat gtatggccct tgtattcaat
900ggcagagacg gaagttgata aggatcttta ttcccatact gctggaatat
gttgtgactt 960tcattctgtt agtgaagctt gtcaattctg tagccgttct
gtggttgtca gacttgctta 1020tacaatttgt gataattgct gttcatttct
taatattcct atagagtctg gaagacatgt 1080acctgatcaa gcttttacag
catccagttt gagttcaaga gcttcatcaa aagctagttt 1140gtattcagct
tctagtaaat cttctaagaa gcaatcagtt cgtgaagatg acaaagacac
1200cgttatatcg ttttctagtg cctctcaatg ggagaacgaa tctgtaatgt
cagactcctc 1260tacatctact ttcaataatg gctttccagg tttgaaatct
aaatggacat taagcaattc 1320tcaacctgta gcatccgact caatgagcca
aatgtcaggc ccgagctatg caagtgctat 1380gggaaataga agacctaata
gagatttttc tagtgctgtg ggaaacagag gacctcaaaa 1440agagtttcct
aaaaattccg ataatgatca ctccagtgat tcgactaccg attatttaaa
1500cagtcaatca tttccggctc tgggaagagg aagaggtttg ggcagaaatc
gacgaaattg 1560atcatttttg actgcaatgg gtcttgttcc acttcataag
attgttaaaa gaatatgtaa 1620ctgtttacat taggcggcta gccaatactc
acattgtctc taaaaccact aactgaacaa 1680ataatcacta actattgctt
ttattatatt acagcgaaat taaacaactt tatgagactg 1740ttttacctta
attcacaatt tttctttata tgtatttgaa taaaatagaa acaaatctaa
1800tacttcctct gtcacttttc tttaaaaggc actagatggc gcctgatatg
ttgatataat 1860gatctaaaat cgcccgccat gtaaaaaaaa caatttgcaa
ctttctgctt taactctaga 1920atactaacct tatttttact atcatttaca
ctaaccatcg taaaatttac tacgctggga 1980aaaaaatgtg gttgctgaaa
aagtatttta tataacgcaa ttttacaaat tattttgaac 2040aaagagatgt
ttctaaaacc tgaaaatcac aaaaaaatat aaattctaaa ttttagaact
2100acactgctga caacaaaggg cgcgatgttc accacaaaat ggacgtcggc
agtgttcgca 2160atgatgagtt gtcattcgac gttttttaga cggacaaaat
tgacacatgg cacgttttga 2220cacagtaggg catattcgtt cttgctgcgg
ctccagattt aaaatatttt caataagtaa 2280ctttaccgag cggttcaaag
ttggccaatt tacacgatgc tgtagccaag gtttagtcag 2340ttgttcagac
aaaatggcca taaatttttt tctggtgatg gtttttttct gctgtcgttg
2400ttggttatga ttataaatta tccaagagtg tatgcaagct atatttaaca
tgccatagaa 2460caggcaaaga ggccatcttg tttttttaga gatgttcatg
tttccgcaca tttggtcaaa 2520ttttggtcaa tccgtacttg gttgcattat
aaaatataat gatatctggc tttcctgttc 2580gatcacattc tactggacca
ttgtggattg tcgacagaag aagcacagac ttatttactt 2640ttgtcttata
ggataccatt gttttttcac catcaaagca aaataaggaa tgaccaattt
2700cacgattttt gagatttatc atctcgggag gtatttctct tttattttgc
cgtagggtac 2760caacaatagt taaatcgtaa ggaggtttta agagctccgt
tgctagtggt actgacgtaa 2820accaattatc catggttacg ttacggttac
ttccatactc accctgagtt aattgtttta 2880cataatattc ttctagaggc
tgtccgttga tgttagttcc atcgaacata tatttggtat 2940ttacgtcaca
cagcatctat tttgagtcca tatttggctg gcttgtttgg gatatataga
3000aaggacatct ttccctaaaa gccaataact actcatcgat tgttaggtat
gatccgggct 3060tataggacgt tttgcagttc tgtataagat catcccatat
ttcacgaatg gctgctgata 3120ggtatgtttc gtttctgcct ggtctagaca
ttttatcatc aaaacgtaga cattccataa 3180gaaattcaaa tctttcccgg
tccataattg ctttttatat cgccccagaa attttgtgat 3240aaagcatttg
tcttgtcgct aaatgattgt ccttttgggc agcagcaaaa ataagtagtc
3300ctagaagtgc atacaattcg tcaatattta gtttcgagtt tgtaagtttt
tcgactttat 3360atttttgtgc cctaacagaa atttcggcat ttgtatattc
tagaattttt tccagtgaat 3420ttggactcat aaaacaatga aaagcatcta
agggagtttg tgcttccttg gttttattag 3480tgaggccttg aacaaaatgt
acgaaattac gttttggagt tttagttgcg gcctttggtt 3540gagaggacca
tctgtgtcca tttttacctt ttagactcga cagcaaccga gtttgcttcc
3600ctttagctgc aatttctgga gatt 36242011520DNALeptinotarsa
decemlineata 201atcactttca ataaattcat tcaattaaag gatttttctt
atttgtgaaa ctcttcaact 60acaattacaa tgcctcctaa gaaaaaacag acttctggaa
atgctttctt ccacttcatg 120caagacttca aaaatagaca aggaagacat
ttcaaaaata tgcaagaggt agccgaggct 180gccagtccgc attggaacag
gatgtcaaag gaacaacgaa aaccatacga agagatggct 240ttaatggcta
agaataattt gcgtggaggt aaatttacct ccgatggttt ggatattgaa
300gtattagaaa atattgagaa ggaagagcag gcaaaaattc aacagatgaa
aaatgatatt 360aattatatat ggaaagtggc gagttcaaat ggaaggattg
cagatgaact tatttttatt 420atacatatca atatatttgt ccattgtacc
tctgaagacc gacattatcc agcagaaata 480gctattgcct gtttcaactt
agaggatggt gttttgccgc aaaatgtttt tcatagaata 540ataaaacctg
gaatgctgcc tttaggctat gccagtgacg caaatgcaca ttctaagaaa
600acccatcagt tacgaccacc gatggctggt gaggacgata ataatattaa
agaagttttt 660gaagaaatga agcactttct ttctaataaa ttatctggat
caagaagaat gccaattctt 720tacaccactg aaaggaatat gaaaatgatt
caacatgttt tagatacgtg gtgttacgat 780tatgacgagg atactcccct
ttttaaagtt tatagtttgg aatatatgtt cagaattttg 840aggaacactg
ttgctgggga tgacgtttgg acaaccgata cattcagtac gagagaaata
900gaaaaagatg tgtacagttt tgctagaggt atttgctgtg agtatcacga
agttaccaat 960gtacccctat attgcagtcg ttcaatttgt gttaggcaag
ctttcaatat ttgcgataat 1020tgctgctccg atctgcgtat caccttatta
cctggagtac acttacctga tgctgctctg 1080acgacggtcc gaagaggatc
tagtagaact gcttcatccg cgccttccat ttcttcccaa 1140tcttcaaaat
atactaattt gaagcaatcc acacgtgacg acgattccga aagtgttata
1200tctttttcga gcaaatcaga atgggataat caatccctaa tctcagaaac
gtcaacttcc 1260gcaactttgg atagcgaagc caattttcct tcacttggag
caagaaagaa agtgacctca 1320tcaccattca acaacttctc ttctaatgac
ttgaacagca gtcagagtaa cacctctagc 1380gtcaaaagtt acgcaggtgc
catgggtaca tcagggttga gcaagcgtgc aatggatatg 1440ccatcatctt
ttaccaacac ggaaagaaat tttgaatctt caaaatcttc agattatgct
1500ccgggccgag gaagaggcgg 15202021554DNAPhyllotreta striolata
202aatatgtgca ttcagcatta acattcaatg tttaaaagtt tcgttattaa
attttgaatt 60taaggaaatc gcaaaccgct tgatttgtta aaatggcgcc caaaagaaaa
gaagccccga 120aaaatgcatt tttctacttc atgctcgatt tccagaaaac
acagggccgc aaatttaaaa 180atttaaaaga ggtttctgaa gcagcaggcc
ctcattggtc taaaatgtcg ccggagcaaa 240gacaacctta cgaagatcgt
gcaagaaaag aaagatccgg cgtcagatac accacgactg 300gcgaaaatat
agatgacata gagagaagag aacgtgaaga acaaatgaga atccaagaga
360tgcaggaaaa aatcaattgc gatttaaaaa tggccaacga ttcgggccag
ctccaagaca 420agctcttctt tatcatacat gttaatattt tctgcgaggc
tcaaggtcgg tactatcccg 480ccgaaatagc tatagcctgc tttagtcttg
aggatggagt ccatccacaa aacgttttcc 540atcatatcat caaacccgga
cctcttccct tgggatatac aggaactgct aagctgatat 600ccgatgaaac
acatcatata ccatatcctc tagtcgacga ctgtgacgac accacagctg
660aggtgtttca tgacatgaaa caatttttat tctcgaaatt accgggtaaa
acagttcccg 720ttttatacgc tgacgagaag ctagcgaaaa tggctcaaaa
aatattagac acctggtgcg 780aagatttcga agacgactgc cgtttcaaag
tctttaatct ccaataccta ttcatggagc 840tgaaaaactt cgctgccaat
gacacggttt ggcctaacgg aacgatgagc gccagagaac 900tcgagaaaga
tccatacgta tacgccagag gcatcgtctg cgattttcac gaaaggaata
960acgtttatac ttattgtagc cgttccatag tgatcagaag cgcgttcgtg
atttgtaaca 1020actgctgtaa gcacttaaac atcgttttaa atcaaggaac
tcacgtaccc gatggagcca 1080tgacgcctgc ttcatctaga gcctccagta
gggccagtac ggtttcttcg agatcctcca 1140aattccagcc ttcgattcat
gatgatgatt cggaaactct ggtttcgttc tccgtcaaat 1200ccgaatggga
cagtcgatcg gagatgtcag aaacgtccga attcagcctg actaatgaca
1260atttccctgg gttggggttc aatccgaaga gcgcgtcttc taattcccaa
ccttattatt 1320caatgtcgca ggaatccaat atgagccagt cttcttctag
aagttacgca ggggcaactg 1380gttcatcgaa atggggcagg gaacctgcaa
atagttctgt tccacagaaa ttttccaatt 1440taaacataca ccaatcttct
agtaatttta acgaaggtga caatccagtc ggagctctca 1500gggatccgag
acgttatcct gctttgggaa gaggaagaag atcataagtt tttt
15542031574DNAPhyllotreta cruciferae 203gacaacccta cgaagacctt
gcaaaaagag accgagccgg tatcagatac accacaacgg 60gcgaaaatat cgaagacgta
gaaagaaggg aacggcaaga acaattggac attcaaaaca 120tgcaagaaca
aatcaattgt gatctaaaag cggccaacga ttcgggccag cttcaagaca
180agattttctt tattatacac gttaacatat tctgctatca taagactcaa
gatcggtatt 240atcctgccga aatagctata acttgcttta gccttgagga
tggagtccat ccgcaaaacg 300ttttccatca tctcatccgg ccaggacctc
ttcccttggg ctacgctgga gaagctagga 360tgatatccaa cgagacacat
cgcatacaga tgcctttggt agacgattgc gatgacaaca 420cggcagaagt
gtttcatgac atgaaacaat ttttaatatc aaaatcacca ggtcccaaat
480gtattcctgt tttatacgct gacgagaagc taatgaaaat ggcgcaacgt
atcttagata 540cctggtgcga ggatttccag gatgattgtc gtttcaaagt
gtacaatctt caatatttat 600tcatggagct gaaaaatttc gctgctaacg
acacagtttg gcccaacgga acaatgagcg 660ctagagaact cgagaaagat
ccatttgtat acgccagagg cattgtatgt gaatttcacg 720atataaatag
cgtttatgtt ttttgtagcc gatccatagt catcagaagc gcgtacgtca
780tatgcaacaa ctgctgcaag cacttaaaca tcgttttgaa tcctgggact
catgtgcccg 840atggagccct gacgcctgct tcgtccagag cgtctagcag
ggccagtacg atttcgtcga 900gatctactaa attcgagccc tcgactcgcg
atgacgattc cgatactctg gtttcgttct 960ccaccaaatc cgaatgggac
agtcgatcgg aaatatcaga aacgtccgaa ttcagcctga 1020ccaatgacaa
tttccctgga ttgggtttca atcccaagag cgcgtcttct aattctcagc
1080cgtattattc gcaagattct aacacgagcc agtcttcttc cagaagttac
gcaggcgcta 1140ccggcgtacc gaaatggggc aacagatctg taaataatgc
tccgtctgct tcacacaata 1200tgtaccaatc ctctagtaac ttcgatgaag
gccacaatag aatcggagct ctgaatgatc 1260cgagacgtta tcctgcattg
ggaagaggaa gacgaacata aaagttttta cccttttttt 1320aacgcagtaa
attttgttaa atttacaatg tatctttaat catttgttca caatgttttt
1380tttttgcaag agttgatttt atttttgtca cattgttatt aaaatgtgtt
tagaagttaa 1440gtttgagtac tgttacattg ttgttgtcag aacaaatgta
aatacagttg taataataat 1500ttaacttggt acacgaacaa gtgtatgaaa
attaatatga ttggtaaagt tgtgtaaaaa 1560aatgttatta tttt
15742041695DNAEpilachna varivestis 204gtcagttgct tctacgggta
attctacgat tgtgtgtatc agtctgtcta ctttttgtat 60taaaggaaat aagttttcgg
gcatttggtt attgattaag tttccaccag aatgtcgaaa 120cctaatgcat
tctacttctt catgttagat ttcaaatcaa aacatggcag aacttatact
180aatatgaaag atgttgctgc agcagctgga ccacactgga aaaacttatc
aaaagataaa 240aaaaaagtat atgaagaaag agcaaaagca gccagaggaa
ctgaaaaact caattcagat 300agaagacctg tggaagagat tgagcacatg
gaacgtgaag aaatgcggta taaacaatct 360atgaaagatg acattacgac
aacattagca actttgaaga gggaaaatgc actaaagacc 420catctattct
tcatagtaca tatcaatagc ttctgccagc atgatcacac taaaatatac
480agcccttgtg aaattgctat tgcggcgttt tcattgcaag atggagtgca
gcattctaat 540gtctttcatt cgatgatcaa accaggtctc gtgccccttg
gatatgctgc tgttgcacaa 600cagcattcaa atgaaactca tcaaatcgat
ttgccgactg acgatgaaga caaccaggat 660aatatattcc ttcaagttat
tgaattttta tcacgtagaa aaggacattc tgaaaaactt 720cctattctat
atgctgctga aaaaaatatt cctatggtaa gtggtgtatt tgaggattgg
780tgcactaaag ttgatataga tccaagtcgg tttcgaattt ataatctgca
gtatatgttc 840aaaatattgc gcaacacagt ggcaggaagg gactgttgga
catcagatac catgagttat 900catgaaattg agaaggatgt ctacaactat
gcttcaaata tagcttgcga atttcacgaa 960acctgtgata tactcacttt
ctgctctcgt tccattgcta ttcgttatgg ctatacaata 1020tgtgataatt
gttgcgaaga tgcgaatatt gaacttattc cggggcgtca cgtgccagaa
1080ggctccatga caactccgga tttgagttac gagattgtga aatcatcccg
aaaaaagaaa 1140tctaccaagt atacatctga tagcagtagt attagggacg
atagcagtga tgttgacact 1200ctaatatcaa atgctagtgc aaggtgtcga
aacattgata gaagaggtga tccaggtcaa 1260acaagtagtt actcaactga
aagttctgca ggccttgcaa ctgctcaagg ggccagtagt 1320agtgttcatg
ctactggaag ttacgccaga gcaacaacct caaacattgg aagttctgga
1380ttcagcaaaa ggcctgcagc tatgcccttc aatgtaacag aagatttgaa
tgctctgaac 1440cttaatccag aatcaatgga tgctcatttt cccactattg
gcagtagcat gggaagaggt 1500cgtaatacta accgaaatac tattctaaag
ggaagaggaa gaggaaaccc gttccagagg 1560actacatcga gatcataaac
tcgtaataaa ttgcccattt gtaatttttt ctttatgttt 1620attttaaatt
cttatgacaa gttcttattg atgaattaaa gaagtttact attgtttata
1680ctacttaagg acaat 16952051314DNATribolium castaneum
205atgcctccta agaagaataa gaaatccggc aaaaacggct attgggagtt
tgttttggat 60tgtcggaata aacatccaaa taagcaaaat atgcacgaag tacaagaata
cgctgcaagg 120aaatgggcgt ccatgtcaaa ggaagaacgc cgcccgtatg
aagaacgtgc cctactagcc 180cgagaaatgt actctcctgc caggtacaca
actgatggta ttgacattga agtggttgag 240agaaaagagc gagatgaagc
aaggaagaaa caagaaatga aagatgacat tacgcgaaca 300ctgaaagcgg
cttatttcgc cactgatctt gacgaaaaaa tattcttagt tattcatatc
360aatcatctgg cgtattaccc tactgaggat aaatatttta tttgtgaaat
agccatagct 420gctgtaagtc ttaaaaacgg agtggaggat gtgtttcata
gaattgtcaa acccggaaaa 480cttccgttgg ggtattatgg aggagcatta
actcattcga aggaaactca ccaaatgctt 540gagttggttc aggatgaacc
ttatgagaac aatactcgtg aagtttttaa tgaaatgact 600tctttcctga
aactttggag aggcaaggga agtgattcta tcgtttatgc ggatgagaaa
660actcatgaaa tgataacaaa agtcattgat aatttttgcc aagaattcaa
ttatcctgac 720gaaattaaag tctacaactt ccaatatctg ttttttgctt
tgagaaactc agtagctgcg 780cgaaccgtct ggcctacaga aacctacagt
tctaccgaac ttgaaaaaga cctctacagc 840tacacacctg atatttcttg
cgaatttcat gaaatgtcgg acatttccgt ctactgtagc 900aagtcaatcg
tgaccagata ctgctacaca ctttgtgacc attgctgtac tgacttaaac
960attcaactag tggctgggtt tcacgtcccg aaaaattcca gaattgcggt
agattcgagc 1020aggactaatt ccaaagcccc ctcagtctgc agcactgatg
attatcgtca agccgcgaga 1080agccgagttg gttcattcag ttcaacatac
gatgagaatt atccagcttt ggggagcagc 1140agatcaagcg cttcttcggt
tgtaagtttc tcctcaatga ggactccccg aaatgtggga 1200caaccgacca
aagctgccac cagaattgaa caatccttca aagccatgtc tgtgagagat
1260caccgcaatc tgaatttccc cccgaaaggc tacgaaaaac aaaatgaaaa ataa
13142061644DNAVibidia duodecimguttata 206aattgtcatt tccataatct
ctgacccaaa aacttaataa aatgccaaag aagaaaataa 60atcctttttg ggcattcctt
atggactata aacgggacaa taaaattgag tcaatcgatg 120aagcaaaggc
taaggctgat gttatatggc gaagcatgac tgaagctgaa aagaagatgt
180atgctggaag atcaaaacag tctcaagcat ccaatgaggc tccattgact
ccgattgaaa 240ttagagacaa agaagaaata ttattcaagc aaaaaatgct
ggaagacata actgccgaat 300tgaatattgc tcggaaatac aacaaattgt
ctagctctaa atttttcatc atacacataa 360atagtttttg ttctgaccat
caaaggaatc gaacatatcc ctgtgaaata ggtatcagca 420gtttttcact
tcagagcggt gtttctaaag atgatgtgta ccattcattt atctatccag
480gttctttgcc gcctggatat tctggtcaag ctaaaattat ttccgaacaa
actcatcaac 540tgccatggga aaacagtaat ttagataatc agcaagaagt
atttgaggaa gttatagact 600ttctgtcaca aagatcacaa ggttcacaaa
aactccctaa actgtatgcc ataagagaaa 660aagaattgga aattgttaaa
aacgttcttg acacatggtg tgatgacttt gatccaaaga 720ttagtttcac
agtgtataat gctcaaacat tgttcacttt gttacataaa aatattattg
780aaaactcacc atgttcttcg gaagctctgt gttatcgtga atttgaaaaa
gatgttcacc 840agtacgcacc aaaaattgct tgcgagtacc atgaagaatc
ggaatgtcct gtttattgtt 900cgagatcaat agcaataaga tatggttaca
ttatttgcca aacttgttct cctagtttgg 960gtattcaact tgtatctgga
tttcacattc caagtaatat gaaacaagct gtaatagaat 1020caaaaaaaac
cttcaagtac cgtgaggttg acagctgtag tacagaaaga gatactgaag
1080atacttgtac tgttagagac gatagtagtg atgtggatac tttggtctct
atagggagga 1140acagtacata tattgaggat tcagcatcgt ccatttattt
tccagaatat gatgatatgg 1200cacaaaatgt ttctagtgac attcgaacag
gaggaagatc gggttttagc cgaaaacctg 1260ctgctttacc atctactgtg
gatagagtat tttctaatct gacattgagc caagattcac 1320tcggacgaag
gaacaatgaa tggccaagct tacgtggaag agggggagga gcaatcccaa
1380gaaccaaagg aagaggtcaa ccgtttaaga ataagtgata cattgaagct
ttacactctt 1440ttctgttcct aaatttctat atttgttggg ctaatcatta
ttgtgttatt tcagttttat 1500ttactgacaa tgcaactgat tgttgttcaa
gaaatgacat tatttcattt caattctttt 1560ttaaattcat taattttttc
tgaaaaattg tactagttta tattattctg ttattgtttt 1620gtttatgttc
atgtttaatt ttct 16442072052DNAHelicoverpa zea 207gttgaaagtg
tcctgtttca gcagttttct ttaatttcac tttgtcagca atttcaactg 60catttcaact
tttcagtctt gctcttgtat tctgtgcatt agcatcagac agaattcaat
120tttggtgtgg tagcgtgtta tcttacgtga attgtttctg gcacagtaca
aaatgccgaa 180gaaagctccc aaaaacgctt tcttctattt tatgctgaat
tataaagacg aacagcagaa 240gaaaggcatc aactatggat cattggctga
tgtctcagtt gcagcgggcg aagtctggaa 300gactgtaaac ccacaagaga
gagcccgttg ggaagccaaa gccaaacaag agaggacaaa 360acaaaatatg
ccagttgcca agtacacgtc taccggggtc ccgctaacgg tcatcgaaca
420gcagcagaaa gaaatacaag aggcaatcca gaatgaaatc gatgatatcc
ggaatatggt 480caaaataaag gcttttaatc aaagcattca ggatgaagac
ttctacgtaa tagatgtgaa 540ctattactgc aaagcgggaa acacgtatgt
gatcggagag agcactgtgt tacgattcaa 600cctgaggctt ggataccagg
atttctatca tgaactcatc aatcctggtc gcatccccgt 660cggctacgca
tcagacgtga aacacggcag cactgagcta ggtctagaca tgccagacga
720aactgaaaac cagtctaact acatagaaat actagccaac atcattgact
acctgaaaca 780gaaggataag aacttgaaag ttctgccgcc tttgtacact
atgcctgaga aagtgctggc 840tgttcaagat tttattctgc aaatgagcaa
taaagcccgt gaggacgagc atatcttccg 900cgtctacaag ttggacacgc
tgttcttcta cctgataaac agcatcaagt ccaacaagaa 960cgagggtttc
cctaaggagt cgctcgctct catacaactc aagaaggacc ccttcaagta
1020tacaccggga ttgggctgtg agcatcacga agccaacgag aaatcaatgg
agtgcacgag 1080ttcccgcacg cagcgctggg cgtacaccgt gatggacagc
tgctgcccag tcgtgggcat 1140cgacatccag cccggacaac atgtgcctaa
ggaatatgat gttgatggca tcctgaagta 1200cagagacgct aagaaggtgt
ggagcggtcc cactgtggct ggatttaatg atgcatcgtc 1260atcttgtaac
tcaacagtga acgattcttt cgaggcccct cccagtctcc ttgaatatgt
1320gggcaaaacg aagcgactcg aagctcgcgt gcacgcgccg caacgactcc
ccgacgtcaa 1380tttcgccaag cctatgctac ctcccgagct gacggaggaa
gccttcccgg cactgtctgt 1440gagccacgga agaggtcgcg gattggccgg
gaggaggaac gtcagaaaat aaagcattca 1500atgccaatct tatttttgta
ctgttatgta acatatctga cattattatt attaatggct 1560gttcctaata
ttctgtctat ccatttattt actcactata gaattttgac atgagcccca
1620tacaagtaat gtaaacttcc tatctctggt aagatcaaaa caaaagatag
ataaaatata 1680gggaacggcc acagacagac gaactacaca atacgtgatt
gaaattatac tttcaaatgt 1740catcgaatta tgcaccttat tgtttgtgtg
ataaagtgca aaattgtttg aaagttaaac 1800gggttgatta tgttatgatg
gaaaactcta ttggtggatt ttaataacca atcatttgtt 1860gttttacttc
ctcgagacag atcattttac actacttgta tggggcctat gtaaacattc
1920aatctctgta gggactgaat caacagattc gttatagaaa ttgctaataa
gagtactcga 1980ttagtaagga taaatgtagt ttgtttgtct acagttaagt
tttgttataa tttcatccag 2040cggtactcaa at 20522081596DNAMegacopta
cribraria 208gtttcttaca ttattccctt atccgtcaat tgctagactg atcccggaat
tcaaaatcat 60gccaccaaaa aagaagcaaa agggtggaag gaacggcttt tattatttca
tgttggatct 120acagaagcaa gaagcactaa aggggaaccg ttactccctt
caagaaattt caggattagc 180aaacccttta tggtcggata tgtctttaga
ggaaaggaaa ccttttactg atttggccct 240taaaaatgcc tctaaaaact
ctggagactt aactaagaag ttcacttctt taggaataag 300ttatgctgag
ttagacgctg aggaaagaga aatagaggat gccagaaaca ttatgattgc
360aacaattaag aatactattg caaatctcga tttaaattct actctgaaga
gtcatatatt 420ctttgtgtgt catgcaaatt atttctataa atctgaaaag
caagtctatt tcccagctga 480agttgctgtt gctgcatttt cccttgagct
aggtgttctt gcaactttac atttttttgt 540tgatcctggg aaaatccctc
tcggttttaa gtatgaatct tcagaatggt cttcaaggac 600ccatcgcata
cctgtcagtg acccatcttg gaaagaggga ataaaagatc cattagaaat
660gtatcgaaga gttaaagaat tcatcaagaa atttacagtt gggactgaag
ttcctccaat 720ttatactttg tttgaaacct cgaactcaca ctatcgtctt
tctgttgtaa aatctgcctt 780gaatatgatg tgcgaagcgg catatgaaga
tacatcctta tttcgagtat attgtctagc 840acatttattt tttgaaataa
gaaataaatg ttctgcagaa atacctagtg tagctattat 900gaagaatgaa
cttgaaaaag acgtgttttc ctatgcaaga gatttaggat gttattatca
960tgaggaaaag gatctctcaa tacattgctc actctctatt gtaactaggt
gggtttttat 1020tatttgtgac cattgctgta aacatcttgg tattaaactg
attctaggaa gacatgtccc 1080aaaagataca gctttattag atagggcaca
ttatgtgaga aagagtgatg gcttatcacc 1140gaaacaaaat gttgctgaat
ccagcagtaa accattgaca ataattgacc atggaagttt 1200aaaagaacaa
agagctgaag agagaagatc tcaagagatg tacttaagag aaagtgaaaa
1260aatcagacct ccaaagtact gttacagtga taaaattggt gctagattta
gtcaaaatag 1320ttcctcacaa ttgtctcctg aaataggagc aacatccaat
gaaaatgaat ggtgtctggc 1380taaaggaaag tcttctgggc gaggtcgtgg
aattttaagt tattcatctc cagaacatcc 1440tcaatggcca atatctggac
gaggacgagc ttttgcaaat tttcataatt aaagatttta 1500ttttgttcct
ataaaaagca aagtggttct aatttttagc gttaatatat ttttgtttta
1560ttaaggaaag gtgacagttc ctattttaat gcgata 15962091574DNANezara
viridula 209acctcgttgt cagacaggca gaagtggccg cgttaccgta aacaagtaat
aacaacaatt 60atagatacca aataatgcca cagaagaaaa aacaaaaagc tgggaaaaac
gggttttatt 120tttttatgct ggaggttcaa aagcaggaag ctctaaaggg
ggttcaatat agtttgcctg 180aaatttctga aattgcaaat cccatgtgga
ctaacatgac cccagaagaa cgtaaaccat 240ataatgacaa ggcatcaacg
agtgtttcca aaaatagcga agacaattca aaaaaatata 300cttctttagg
cattagctat gccgatgtag atgctggaaa gagagagttg gacgaagctc
360gtgatcttat gatagcaact attaaaaaca ctgtcgcaaa tcttgacatc
agaacatctc 420tgaagactca taagttcttt gtgtgccatg ctaactattt
ctataagtct gatcatcata 480tttattatcc tgctgaaatt gctcttgcag
ccttttcttt ggagttgggt gttcttggca 540ccattcattt ctttgtagat
cctggaaaaa tacctcttgg tttcaaatat gaagcggcag 600attggtcaac
tagaactcat ggaattccag taaatgatgc tagttggaat gaagggatta
660aagatcctct ggaaatgttt ggaatggttc agtcgtttat caagaaattt
actgagcccc 720ctgaagtacc tccaatctac acaatgttcg atactttaaa
tgctgacagt cgtctttctg 780ttgtgaaatc tgcattgaat atgttgtgtg
atgctgcaaa tgaagattcc tctctatttc 840gtgtctattg tttatcacat
ttattctatg aaattagaaa taaatgttct gcagaaatac 900caagtgtagc
cataatgaaa agtgaattag acaaagatgt tttctcatat gccagggacc
960taggttgtta ttatcatgaa gaaaaagatc tttcgatgta ttgttcgctt
tctatagtaa 1020caagatgggt gtttacaatt tgtgatcatt gttgcaaaca
tctgggtgta aagctcatcc 1080ttggaagaca tgtgccaaaa gatacagatc
tttcagaaag tgctcgattt atagatgata 1140aacaatcttc taataagtta
gatcatggtg cttcttgtag taaacccttg accatcattg 1200accatggccg
tttaaaagaa caaagagctg aacaaaaacg ggcgcaggat atgcatttgc
1260gtgcaagtga aaaaataagg cttcctaaat ccagttatag caacaaaatt
ggtgccagat 1320tcagtcaaga aggtgccagt agttctcaaa gctctctgga
aacaaaagaa gaatccaatg 1380aaaatgaatg gtgcatcgta aaaggaaaag
gatttggacg cggacgtggt ttcactgtag 1440aaaaatcttc agctgaagag
cctatctggc cagtttctgg aaggggtcga gcatttcaac 1500aataaatttt
aaaagacatt ttaatataga caaaaaattt taataatttt gatacaagtt
1560ttttatactg tttt 15742101640DNAEuschistus servus 210tagattcacg
tttggttgaa acctctaaga ctgacagacc aaaaaggccg cttttgttta 60acttgtttat
atcgttcacc caggactgtc agtatgccac agaaaaaaaa acaaaaagct
120gggaaaaatg ggttttatta ttttatgtta gaagtacaaa agcaagaagc
tcataaaggc 180aataagtatt ctttgcctga aatttctgaa attgcaaatc
cattgtggtc tgctatgtca 240ccagaagaac gtaagccata taatgacaaa
gcactaatga acgtttccaa aagtaatgaa 300gacttaacaa agaaatatac
ttctttggga attagttatg ctgaactaga tgccgaacaa 360agagagttgg
atgaagcccg aaatatgatg attgctacaa ttaaaaatac aatagcaaat
420cttgacccca gaaattctct gaagactcac acattctttg tatgccattc
taattatttt 480tataagactg atcatcatgt ttattatcct gctgaaatag
cttttgctgc cttcaatctg 540gagcttggtg ttctagccac cctacatttc
tttgtagacc ctggaaagat tcctcttggt 600tataaatatg aagctgcaga
ttggtcagct aggactcatg gaattccagt aaatgatcct 660agttggaatg
aaggggtcaa agatcctcta gaaatgttca gaagggttaa ggatttcctt
720aagaattttg ctggagactc tgaagtacct cctatctaca caatgtttga
taatttaaat 780gctaacagcc gtcattctgt agtaaaatct gctttgaatt
tgatgtgtga tgctgcaaat 840gaagattcct ctctatttcg tgtttattgt
ttaccccatt tattctttga gattagaaat 900aaatgttctg caggaatacc
aagtgtggcc ataatgaaaa gtgagttaga taaggatgtt 960ttttcatatg
gcagggatat aggatgttac tatcatgaag aaaaagatct ttctatgaat
1020tgttcacttt ccatagtaac tagatgggta tttaccattt gtgatcattg
ttgcaaacat 1080cttggtataa agctcatcct agggagacat gttccaaaag
atacagacat ttcagaaagt 1140gctcgattta tagaagataa atattctcca
aataagcttg atcatagtga ttcctatagt 1200aaacctttaa ccatcattga
ccatggacgg ttaaaagaac aaagagcaga acaaaaaaga 1260attcaggata
aacacttgcg tgaaagtgaa aaaattaggc ttccaaaatc tgtttacagc
1320aacaagattg gtgccagatt cagtcaagat tcaggcgcta atagttctca
aagctctttg 1380gaaatgaaag gaacaaaatc agaagctaat gaaactgatt
ggtgcattgt taaagggaga 1440agcttagggc gtggacgtgg ctttatccca
gaagaacctt caactgaaga gcctaactgg 1500ccagtttctg gaagaggaag
agccttccaa taataattta tttgagaaat tttaatatag 1560acaaaaaact
ttgtaatttt aatatggacg aaaaagattg ttatttttaa tattttttta
1620tgtaacagtt tactatactg 16402111500DNAOrius insidiosus
211ttctggcttg caacttgaaa aaaagactcg tgattttact tgtagtctca
actctcaaga 60gtcacgctgg aaaagtaaag ttgagctgct tatttgttgt caacgtacca
tttttcgtaa 120tatgcctaat aaaaagaaga ataaagccgt gagaaacggt
tatttttatt ttatgcaaga 180tgtccgtgaa cgcgaggcac tgaaaggcca
ttattttcca agtcaaaagc aagtcgccga 240tttttgtgct ccactttggg
cgaatctctc tccacaagaa aaggaagatt acaacgatca 300tgccaagcag
aaaaggcttg aagagaaaaa ttctgtcgat gaagctgcca ctaagttcaa
360ttcaatggga atatctattg cccatcaaga cgctgaatta cgtgaacaag
aagaaaacga 420aatgattatg aaaagtcaca ttaaaaactt aatgaccgat
tgtggaaaag ctggaattat 480gacgtacaag ttttatttgt gccatgtcaa
ctacgcattt aaagtcggcg ataagaagtt 540cgctcctgct gaattagctt
tggtcgaatt ctcactttat gagggaatca caaatagatt 600tcacactttt
attcatcctg gaaaaattcc tttggggctc agatacgagg cgatcgaatg
660gtccaatatg tcacaccaaa tacctgttga gggtggattg ggtgaaaaat
tggataaaaa 720tatcttcaac tccatcatct cattcttaaa tagcaaaggt
agaaataaag cgttgccggt 780tatttacacg atgccagatt cgttaaattc
aaacgattct atgaaggccg tcgtgagtgc 840ttttcagcag ttatgcgagg
tggctgaagc agaccctgat attttccgca tttatgaatt 900acctcatttg
ttctacgaac tccgcaataa atccgtagaa gacggccaag tggattatag
960atttccgagc gttgccttag ccagagagct tttacaacgt catgtttata
gctcttgtag 1020caatatggga tgtgaatttc atgttcaaaa tgactatgaa
ttgaaatgct cattgagtgc 1080tgtgacccga tgggcatacg ccatttgcga
tcactgctgt caatatttgg gaattaaagt 1140tatccctggt gtacacatcc
ctatccccac cctactgggt aacgaaggat attctgctgc 1200tgtgccttgt
gtatcgccaa aaggcaattt gccaactcat gcttctcttc ctgaacctgc
1260ggaagttcat gaacaattgt cttctgattg ccctgatgaa tcttttggat
ataaatatga 1320tgaagacgat ttccccggtt taggtggcaa ttcaaagaat
gtaagaagtg aaaacgcccg 1380ttcttggggt aatccatgga gaaaatgatt
ctttttatat taaaactttt aataaataat 1440ttacctttct ttatagacaa
agaaatacat ttattttaac ttctgaattg tttaaaaaaa 15002121810DNAManduca
sexta 212tgctattaat tttagctcct tacgatttac gaattggcat tttcaactaa
aagttttaca 60gctatatatt tgaaatatat atttataatt gaataagatt aataaaggtt
gactttagac 120aattattcta aatttaaact ggtaccaata ggaagatgcc
gaagaaaccg ccaagaaatg 180cattctacta ctacatgctg gacttcaggg
aaaaccagaa gaagttggac atcacttacc 240aaagtctaaa ggaggtggca
gaagctgcag gaccatcctg gaagaccatc tcaccaaccg 300caaaagccaa
atacgaagct atggctagaa aagagagggg tacaaaccag aagttcacat
360ctactggtgt gcctctcgcg tatttggagc agcaacaaaa agaaatgcag
cttgtggagg 420aggctgaact caaagatatc aagaacatta tcaatcttaa
gtgttttaat caaagtattc 480tggatgaaga cttcttcctg ttggatgtga
ttgagttgtg caaagctgga ccggactatc 540tcattggaga gttcactgtc
ttacagttta acctgcgtga aggcattaag aacagttatc 600acgagtttgt
tagaccagtt agcgtgacta tactgaccta cggccttgac acgctggacg
660acagcctacc caaggagaac tacgtgcaga tcctggccaa catcatagac
tatctgaaac 720agaatgatcg tggcacgaac acactaccgc ccatattcac
aatgccagag aaagttgagc 780cgatacagaa ttttatatgg cagctgtgta
acagagctgc cgaggacgac gcgttattcc 840gcgtatacaa gctggacacg
atgttcttca cgctggccaa cgcgattcgc agcggcgaca 900acgaggggtt
cccgaaggag tcgctcgcca ccgcgcagct gaacaaggac ttgttcaagt
960acacgcccgg gttggcttgc gagcaccatg agaccgtgga caaaagcaac
gagtgcacat 1020cctctcgcgt gaagcgctgg gcatacacaa tcctggacgt
gtgctgccca ctgctgggca 1080tcgccgcgca gcccgggaaa cacgtgccgc
acgacttcga tatagacagc atcctctcgt 1140acaaggaaga gaagcgtgaa
cgagcgctac cgagtgtagc tcgtcggcag gtgccgcacg 1200acagtatgtc
gtcttgcagt tcgtcgatca tcaacgacac agtcgacgat tccaccgtgt
1260atagctttaa cgcctcccac accagtgctc caaagggcaa gaggacctac
acgccgctgc 1320gtatgccgag gactgattat tcgcaacgtc tccaacaagc
ccccgagctg accgagacga 1380acttcccaac gctcggcagt acaggacacg
gcagaggtcg cggcctcgcg agcagtttcg 1440atcggatgaa cttgaagaag
taaataaaaa ttaagaataa cttgcaatgt gccaacgtat 1500caacaagatt
tcattgtgaa tttgtaataa gctaaaataa atgactattt ttctgtgttt
1560tatgaatctc ggtgtttaag tatacatggt gctgtaaaag cgtaaatatt
gaacttttca 1620gggcacgctg tataacgtag tagttgtgac attgtttata
cgcacaaaaa gaactatcaa 1680aaaacttagt aaatatgtct taagtcatat
ctaaatttgt tccatcatca ttgaacatat 1740tttgtaccag agtgaataga
ggcacagtca taaactcgcc atgttgataa cttcatacaa 1800ttggaaacgt
18102134514DNASpodoptera frugiperda 213cgggaattgc acgaattgaa
ccggctacaa aaaaagacgt tttgcgaaat agaaattatt 60ttgcgttgtg ccttgactgc
ttagactgca ctaagtgtct gcatctgcga acgaaaccta 120cccattgtac
tttgtaatta atcacctgct atattatttt tttaattcaa ttcgactaat
180atgcctaaga aaaagaccgc ctactggttc ttcttacaag atttcaaaga
agaacaaagg 240ctaaaaggca ttacgtatac ggaaaaacag gaattggata
aagatgccga taaagcatgg 300aggagtctac ctccttcagt tcgtgctaag
tacgaacaaa cggcgaagaa tgagaaggaa 360aaaaacaatg taactgttat
aaagtatacg tctaatggca tcccgttgac tgtgattgaa 420aaacaaaaac
aagaacacga gatggcgcaa atcaatgagg ttcaagatat tacaaatata
480attaaaacga agtccttcaa ccaagaaatt ttagatgaat acttctacat
catcgacgta 540aattcttatt gtaagtggga tgacaaatat ttgattggcg
agtgtaccgt tttgaggttc 600aatctgcgga atggtattcg agattattac
ccagtattga tcaaccctgg agaaataccg 660atggggtacg cgtcggatgt
aagagtggcg tgtatagagt tagggctgga catgccggat 720gcagaatcac
cgagtaacac gatggacatc ctcgccaata tcatcgactt ccttagacag
780aaggacctga aggccggatc tctaccgcct ctgttcacta tgtcggagaa
agtactgcct 840actaagagct tcctggaaca gatgtgctac acagctcatg
aggatgaaat gatcttccgt 900gtgtatcgcc tggagaagtt gttgttccat
ctggtgaata gcctgaagtc cgacaagtac 960gacgggttac ctcatgagtc
tctcgcgctc gagatactga agaaagactg tttcagatac 1020agcgaaggac
tggcttgcag acaccacgca gccagagaca aggccacgga gtgcacggac
1080tcgcgcgtgt tccgctgggc gtacagcatc atggacgagt gctgcgcccc
cgcaggcatc 1140actccccgct ccgggaccca cgtgccaaag gacatgaaca
tcgaaaaata caagcttcta 1200aggaacccta ctcctagagc tacagtcgct
ggatatgagg cggcaccatc gtcctccaac 1260accacggcca acgattctat
gtgggaaggg agctttgaag cctctctcgt cagtgagccg 1320tcgggagctt
ggggtgaacc ttctggagct tggggtgaac cttctggaag tcaggcggcg
1380agtgcctggc ctcaacccac tggcattccg tcgtcggaaa cctggccaca
actccctggc 1440agtgggccgt cgagtgcctg gcctctacct tctggtagcc
agtcgtcgaa tgaatggccc 1500gggctccctc ggagtcagtc gtcgagagcc
cgcggtcaac actccggcag cggtcgtggt 1560ggccgggaat ctgagtctgg
gaggcgaggg cgccgcacac acgagcctat gcgcatgccg 1620caagccgact
attcccagaa cctccaggcg acgagcgaat taatggagaa acacagtccg
1680ctcctaccag gccttggacg tggtcgcaat ctagctggca gcttgagcaa
gatgaacatc 1740aataagaaaa agtaaagcat tgaatgccat ttaaatttct
accctattat tattatgtaa 1800tgaatctgat ttactttgta agtatagtgt
tgtcagatgg gctgtgttta caccgactta 1860ggtggacaac acaaatatca
tacaggaata tcgggaaaaa atacttggaa ccagtggcgg 1920aataaattga
caacccggca aagttctcag taactcaatt cttcttttta tattaggaga
1980ttgggctcat ccgatgaatt tgccacgggt ctcgaatcat acagttactg
cttaaaattc 2040tatttacatt ctacgactat agagctatgg acaaaaagtc
tattgattta tctctttttt 2100aatctataat ccataaacaa tattgacaac
tatcctacct cacaactatt tgaccaaatt 2160gctttaatat cttaaatcta
ttgacaaata actagtaatg tataaaaata ttttcctcta 2220tcatattttc
aaaagagtgt agtttaaatg aaactatatt tttagacatc tatcccctaa
2280tcttataaat taagccatgt aagtctagga ccattacatt acattgacca
actttttcca 2340tagctccaat tattaaccat acgtatctat agcacaatat
agcttaacga tagctaagcc 2400catctggcta cacgaaaaca tgattatttt
gtattacaat ccgacgatac tcaatctttt 2460cagtctaact gctcaatgta
tatgtaaata atgtggcaaa caataagtca tttatatatg 2520taattgactg
attaggctac ataggattgc ctaaatatat ttaagatata tagattgtat
2580tggtatgtag gtacttaata aggaaaactg aggaacctta agactagtga
agtgtgatat 2640taggatggtc taggagtgta ctaagtacgt tttgaaaagt
caaaattttg agggaaggct 2700ggtttattaa cgtattgaca ttcgatgtca
ataaattcat tttatttgga tttaatcctt 2760taaacttacc tacaatttga
tacgggagta tcaaggtagg attaaaattt aaaataaaat 2820ttgaataata
ttcaaaatta aatggtttac ttcattaatt acccattaaa gttaactctt
2880cgagtcgttt gcattacggg tatttcgttg ttccttttcc gtaaaacatt
gagtatcgtt 2940ggatttactt attaattaaa aactttttga tatttttata
atgtgggatt tatgctaatc 3000tccgtacatt ccgtattcat attgactgtt
ggaaagccaa tttgtaaacc cggaattatc 3060gtagatccca catgtttaga
atagttctga caatcggaac ttaaaaattc tgaaagaaac 3120cttttacacg
tgttatattt tacactatcg tctatcgttt tagtaaaact tcgtgcaggg
3180ttgacaaaga gttagttata agtataattt tgttgctttt cattgaaaac
atattttatt 3240taccctaagt tttatataag ttttcaactg ggctaagaat
gtgactacat ttacataaaa 3300ataagaagaa attccaccaa agttctctcg
tttgtctggt atagattcag tatcgaatgg 3360ttaaatgaca ttacacattt
attaaagatg tcttgagaaa taatcagcat cttattaaaa 3420agtataatta
aataatgttc aatgttcata tatgtctatt gtagtggttc cagaattccc
3480cccaccgacc tgtagtacgt agatgcagaa tagacacaaa atatctcaga
tgttgtacga 3540tttagctgac cggtcaatat agctcaccac gccaaatttt
tagtgccacc tgtgctcaaa 3600aacttaacct aatcagttgg ttatatcacg
tattccttca gtacgagcat tactttgtga 3660tcgtcttaaa tagtggtatc
tttgtctatc tagtcggaat gaggcagttt tctcattata 3720ttcagtagta
tatagtttgg acaaaatctc aatgttggta gtgtttagta tacgtaagaa
3780agtctatata aatcgcggtg atttaaaaaa aaaaaataac caaatgatgc
tttggtccag 3840aacttgcctg agttccaagg cttcgaacat ataacaacat
gttcagtaaa atttaaattc 3900tttctgtgtt ggattgccgc gagtccagtg
ttacattcat agggatatac ccttttaata 3960ctataagtac tatcatagtg
taggtatcaa taaatatgta ttttgatatc gtatttatta 4020atctccttta
ctcgttttga aagcggatat tatagaaaag ggggtttata aacccaattt
4080ggacaagaga tataattgac aatgtatgtt tcccgtcgaa attattttga
atagtatttg 4140tagttatata aggtacataa tacctatata caattttata
tagttcttcg catgtggcgt 4200tggtttattt aagggtaata actatataac
ataactacag tcatagatat catatttacc 4260tatattcaaa tataataaaa
tctttcaata aactatgtaa tgttttctgg ctcaataata 4320tttgaaaatg
gcaacctcta agtcaactag cgccacctat atattaaagc ttaatatgtg
4380tgatatgtat acgtagtagt gtgtttatgg atataaaacc tgtctgactg
tagattacaa 4440atatctatta cgaattctat gtgtgatagc cataggtacc
tatgtagtac tttcaatgtg 4500tgtttaatga ataa 45142142917DNAOstrinia
nubilalis 214aaaaatttgg ttggtttgca aaaattcgct tgtgccggca aatttatttt
gaccaccgtc 60tcatattcgt tatattgtaa tattttacat actctctgtg ttattctact
ctccgtgacg 120atacgatagc catttgagta gggcatttat ttcaatatgc
cgaaaagaca agtaaggaac 180gcattttatt tctatatgat tgacttcaga
gaacaacaaa agaaaattgg aattcagtat 240gggaacataa gagaagtctc
tgaagctgca ggtccttcgt ggagggaggc accaccaact 300gtacgcgcca
agtttgaaga aatggctcgc caagaaaaat tgaaaagaaa tgtacctgac
360cagaagttta cttcaacagg gataccattt tctgagatag aacgacaaga
gagagagttg 420cgtgaagcag aggaagccga gagagaatac attaataatg
ttgttaaact aaagtctttt 480aattcagaaa ttatacatga gtacttttat
gtgatggatg tgaactacta ctgtaagttc 540aactcggatt acctaatagg
ggaatgtacc gctctacgct tcaatgttaa aaatggcatc 600aaagatcaat
ttcatactat gatcaaccca ggtccaatac cagttggata tgcttatgat
660gtcaaactcg gagctcaaga acttgggctg gatatgccgg atgcaattac
ttataagaca 720ttgccataca tagacacttt agccatcatc gtcgactacc
tgaagcatgg ccttccgact 780tctagcaaga cagtaccacc gatttacaca
atgccagaga gggtgcaacc agtcctgaat 840tttatattgc aaatgtgtgc
aaagacaggt gaagacgacg gcatgttccg cgtgtacaag 900ctggacacgc
tgttctacac gctgctgaat ggcgtcaaga accgcggcga cgagggattc
960ccccgcgagt cgttggcgct cgcgcagctg aagaaggacc cattccggta
cacgcccggc 1020ttggggtgcc ggcaccacga ggacatagac aaggctgcgg
agtgcactac ctctcgcgtg 1080aagcgctggg cctacaccat catggacagc
tgctgccccg tggtcggcgt ggacatgctg 1140ccgtcgaagc atgtccccgg
cgacttcgac ctggacacca tcataaattt tagagagcaa 1200aagaaagaaa
gagctgggcc gtcagtggct gtccctcggt ggggggaacc ttctaacgcc
1260tcagccagcg atatcaatca gtcttcgttc aacgagtctg ctaatacttc
agcctcaact 1320tcagctcctt cgacttcatc aaagaaggaa aaaaaagccc
agcctcctct acgtatgcca 1380aagacagatt attcgcaaac ccttcgccca
gcgcccgagc tcaccgaagc caacttccca 1440agtttctctg ctggacgcgg
ccgtggctta gcgagcagct taaacaaaat gcatattgga 1500aaaccaaaga
agtaaacgta gacagtctac ctttaatctt gtcttgacct gtactcaatc
1560ttgactgcta actttatttt aagacttcct cttgtgctgt gggacttaga
tacaaatatt 1620ctattaatgt ttacacttcg tgtatctaat acacttaagt
caggacttgt taccctttta 1680ggattgtgtc tacaaaatta ttgggcgagc
ttatatggcc gacagggtat ggtcatttaa 1740tgggtgaaag ttcgatatgg
atatttctat aatgcagcta ttcatcacta tcgaatatgt 1800atttagaagc
taaattttag ccatgtatgc tcgctatcgt gcattatggg tgcataaaaa
1860ataaaattag tctcttgata tgacaccttt acatttcggt cttggttcga
cttttacgtg 1920catttatctg ctatgtatgt ctgatatgga gcaaagcaag
tattaataaa atatttcata 1980gtaaataata gaatattcag tagtaaataa
ccgtatataa tatacctaat aatactatat 2040tcctgtgtat aaagcttgga
tacattgtgg tgaaatgacc ctcagtgacg cacaacgctc 2100gaactacttg
acgccacgtt gtcgcactgt gtcaagcatg acatagattt acttaacatt
2160ttcctttatc cgagatacca cttcatttgt ttcctagtgt gtccaatgtt
ttaaattgac 2220tcaacaaaaa gatttcaaaa attgtcgtag
cgaatgattg ttaatgtctc tagtgtatcg 2280gcgttcaatg acttaagtat
attgctatct ctagatgatt gtctatttca ttgatcaaat 2340atgagaatac
atgattatgt tttatgtcga tgtgaggtta taagtaaatg aatagcattt
2400agtattaaat ttaactaaac atataaaata tgatattatc tctacatgtt
atttgatgtt 2460gtaacaatcg atgtgataaa taatattaag tcaaatactt
ttgtgattgc tttagttatt 2520taagcattag tgccatttcc gcttcctctt
ctctgagata taattttcga aagaaacaaa 2580actattttta tatttcgttt
attaaatctt taaaagaagt gtatcatatt tttaaggttg 2640gccaagtaga
ataagtttta atgtaatcac caaagtgata cttgatctta atgttgatat
2700tatttcggtt ttttttacga taaagactct agcaaacagc cggctagagt
gtaatgtgaa 2760gtatacgtac ttattgatag ttagaaccta ccgctttgtc
gcactcgtgt gggctgtgct 2820agaggcttta gtaactaagt ttcttatgtt
aaaatgttga agtatttgtt tgtacttgaa 2880tttatagttt cgtatgacaa
cttaatgacg attaaaa 29172151713DNALygus hesperus 215accaaagttc
aaagcattga atactaacgt cgttctgagc aattcaaagt caaagagtgc 60atgaaactgc
tgcagatgtg tgtcctctgg ggacaccttg ccctgacgtc caaactcccc
120actacaccaa ggataaggat caaggatcgc taaggataac ggaactccat
ttgaacagtt 180gacttacctc tctgcaatca tgggcagtaa aaacaaagga
aggaacggat tttactactt 240catggtagat ttgcagaaag aaaaggctga
cgatgggcac ttctatagaa tggatgagat 300gtcaggaatt gctggcccac
tctggaagaa tttatcgctg gatgaaaggg aatattacaa 360cagaagggct
aaggaagaaa aattaaaagg cgcctcggat atggatcgca agtacaattc
420tctgggagtt agtttttcgg tcgtcgaagg cattgagaga gagttggacg
aacaggagag 480agtcatgaaa gctaccatca ggaatattgc acagtcctcc
agtccggagg cactcatccg 540aaaacctttt tatttctgcc acgtaaacta
ctacttccaa cccgataaag atagctacca 600acctgcagaa attgctctcg
cggaattcac cctggaagac ggccttcgag agacgaggca 660tttcattctc
cacccaggta aaatccctct ggggatgaaa gccgaggcac aaacctgggc
720tgataaaact cacgggatcc gactagtaga caagcctggc gaggaaatcg
aacgtgaagg 780agacttcgtc aaagtatttt cgaacattgt gaacttcttg
aagaacgacg ctggagtggg 840gcagggagga gggggaatgg tgctcccagt
gatgtattcc atgccggatt cgctgaacaa 900caataccagt ctgtctgctg
tgaagtcctg catgaacttc ctaagtggat gtgcccatga 960gaatcgcgat
ctcttcaggg tctacccgct accagagttg ttctatcatt tgtggaagaa
1020gttcgagaag aagggcgacg aaacacccac ggttgctgtc ttggagaacg
aagttgaaaa 1080agacattttc agtggcgctg ctgatttagg gtgtgagttc
cacgaagcga aggacctgag 1140cctccactgc agtctgtcaa tggtgaagcg
ttgggtgttc acagtatgcg atttcgtcaa 1200caaaatgacg ggtatagaga
cgattcctgg atgtcacgcg cctgacaatg cggacctcca 1260atgggctgcc
atacaatcca gcaagcgcat gaacccagac gtgaaagttg gatacgtcga
1320tccaccaccg ccaaaatctc ctcccaagtt tgttgatttg acgaaaatgc
aggaactgag 1380gatacgagaa aaagaagctg tggaagaaga actctcccaa
gccgaacgtc ttccacctcc 1440tgtctctgct gtcttcagag gagggccgct
ccctcctaat ctcaatgata gagatttccc 1500tcctattgga acgagtggtc
gcggaatgta cctccccaga tccaagtggc cagcagtctc 1560ttcgcaaaga
gggaggcgtc agtaatgaaa gtggaagaac ctatttcgaa acctacgcga
1620ctttggttga ttataaacca atctaccctc gatttattgt tcatttttca
attgttttat 1680tgtttattcg tgccagaatc gtttaggata ttc
1713216601DNAPectinophora gossypiella 216tcaactttca gttcagttcg
ttcgttcagt cagttgattg gaaaaaaaag ttagttctaa 60aatctaaatt atttttgaag
gaaaaagttg atcaaaatgc cgaaaaagcc accaagaaat 120gcattttact
attttatgtt agattttaaa gaacaacaaa gaaagaaggg tataaactat
180gggaatatga acgaagtagc gcaggcggcg ggccccgaat ggacgtcagc
aaaaccacaa 240gtgcgcgcga aattcgaagc gatagcgaag gctgagaagg
cgaaatcaaa tgtgccggag 300cagaagttca catcaacggg gcagtcgctg
gccgagctgg aggcgttgga gaacgagcgg 360cgcgcggccg agaaagccga
ggagcgagac atcctcaact ttgtcaagca gaagagtgtc 420gacggcagta
tactggacga ggacatgtat ctcatggatg tgaactacta ctgcaagact
480ggatcctcat acctgatcgg ggaattggca ctgctgcgct tctctatcag
agacggcatc 540aagaacactt accatgagat cattaaccca ggtggcatcc
ccatggggta cgcgctggac 600g 6012171396DNADiabrotica barberi
217aaaacattca ccacatcaac acgtgaatta atattcgaaa atggagtacg
aaaatacaca 60acaaaatata aacttcgtac cgtgtgtaag atgggttaaa cgaggagtgg
ccaattcaag 120cccagtaaaa ttgcaactgt cgaaaaacga gctggctcaa
attattaatg acaccaagat 180taaattacaa gaatccaatg aaaatgaaga
cgagcccatg gaagaaggtg aaacgtctca 240aacagatgag tttgccttag
aggattacga taaagaagac gaaaatgagg acactgcaaa 300tgctttagga
attggatcat tggcagaact cgataatgat gctgcagaca atttttctga
360gtcagacgat tctgaaaaag aagatgataa aatcaaacca tctgacaatc
tcatactagt 420aggacatgta gaaggggatg caagtctatt ggaagtctac
atatacaatg aacaagaaga 480gtcattgtat gttcatcatg atattatgtt
atcatccttt cctctgtgtt tagaaccgct 540aaactatgaa ccgaagatgc
ccaaaggaaa ttattgtgca gtgggatcaa tgtcacctgt 600tatagaggtc
tgggatgtag atattatgaa tgttattgaa ccttcattta ctttagggag
660acctgctagt aaaaagaaga acaaggaaca tataggccat acggatgcag
tgctttcatt 720agcttggaac aaaacctttg aacatgtgtt ggctagtgga
tctgtagatc aaacaataca 780cttgtgggat atggaaatca aaaaaccaag
tacaaccatt aaatccttcc aagaaaaagt 840tcaatgtctc gaatggcatc
ctttagaggc acaaacactt ttaggtggag gttgtgacaa 900atctgcaaga
gtatttgatg gcaggacccc tgaaacccac caaacttggc tacttgatgg
960agaagctgaa aggctatgct ggaacccttt agaacctttc acatttttgg
caggtaccag 1020cagtgggtct gtacagtgtt tcgactgtag aaaaggacag
ctatggtcag ttaaagcaca 1080cagcaaggaa gtaacagggt tagtccttag
caaacaatgc caaggattgt tgattacttc 1140ttctacagat gaaacagtta
aaatttggga cttggctaca ctggaagctg aacctaagct 1200tgttaatgaa
aaggagttta atatggggaa tattcactgt ttggatttat gtccagactt
1260gccgtttgtt atatctgtag gtggagataa gaagtcaaat aattttactg
tttttgatgt 1320acagaatatt gatgttgtta aacacacatt tgggccaaga
ggattggtac agctagttcc 1380agatacagaa gaaaat
13962181402DNADiabrotica undecimpunctata 218caaacatgta tataaaaaca
tatttcaaca cgtgaaatat tcgagaatgg agtacgaaaa 60tacacaacaa aatataaact
tcgtaccatg tgtacgatgg gttaaacgag gagtggccaa 120ttcaaatccg
gtaaaactgc aattgtcgaa aaacgagctg gctcaaataa ttaatgacac
180taagattaaa ttacaagaat ccaatgaaaa tgaagatgag ccaatggaag
aaggtgaagc 240gtctcaagca gatgagtttg ccttagagaa ttacgataaa
gaggacgaaa atcaggatac 300tgcaaatgct ttaggaattg gatcattggc
agaacttgat aatgatgctg cagacaactt 360ttctgagtca gacgattctg
aaaaagaaga tgataaaatc aaaccatctg acaatctcat 420actagtggga
catgtagaag gagatgcaag tctattggaa gtctacatat acaacgaaca
480agaagagtca ttgtatgttc atcatgatat tctgttatca tcgtttcctc
tgtgcttaga 540accattaaac tatgaaccaa agatgcccaa aggaaattac
tgtgcagtgg gatcaatgtc 600acctgttata gaggtctggg atgtagatat
tatgaatgtt attgaacctt cctttacctt 660gggaaggcct gctagtaaaa
agaagaacaa ggaacatata ggtcatacgg atgcagtact 720ttcattagct
tggaacaaaa cctttgaaca tgtgttagct agtggatctg tagatcaaac
780aatacacttg tgggatatgg aaatcaaaaa accaagtaca accattaaat
ccttccaaga 840aaaagttcaa tgtctcgaat ggcatcctct agaggcacaa
acacttttag gtggaggttg 900tgacaaatct gcaagagtat ttgatggcag
gactcccgaa acccaccaaa cttggctact 960tgatggagaa gctgaaaggc
tttgctggaa tccattagaa cctttcacat ttttagcagg 1020taccagcagt
gggtctgtac aatgttttga ctgtagaaaa ggacagctat ggtctgttaa
1080agcacacagc aaggaagtta ctgggttagt acttagcaaa caatgtcaag
gattgctgat 1140tacttcttct acagatgaaa cagttaaaat ttgggacttg
gctacattag aagctgaacc 1200taagcttgtt catgaaaagg agtttaatat
ggggaatatt cactgtttgg atttatgtcc 1260agacttgccg tttgctatat
ctgtaggtgg agataagaag tcaaataact ttagtgtttt 1320tgatgtacag
aatgttgatg ttgttaaaca cacatttgga ccgcgaggat tggtacagct
1380agttccagat acagaagaaa at 14022191436DNAPhyllotreta striolata
219aatttcaatc gaaaaaacat tgcacgtgaa gtaaaacaaa tttttcaatt
tcacaatgga 60aatagatgca cagacagaat caaatataaa cttcatacca tgcgtaaaat
gggtaagaaa 120aggtatagct aacacgaatc ccgtgaaact ccaactgtcg
aaaaacgagt tagtcgatat 180aataaatgaa acgaaagtga agctccaaga
agctgaagaa aatgacgagg aacttatgga 240aagcgatgta ccgagacaag
atgaattcaa tctggaacaa tacgatgaag atgaagccga 300agaaaacact
gcaaacgcat tagggatagg ctcgttagct gaactagata atgcagccga
360ggataatttc tccgagtccg acgattctga taaagaagac gaagtcatta
aacctacaga 420taatctgata ttggtggcgc acgtagaagg agacgccagt
ttcttggagg tgtacgttta 480caatgaagaa gatgaatctt tgtatgtaca
tcacgacatt cttttaccat catttccgct 540ttgtctcgaa cacttgaact
acgaacccaa aatgcctaag gggaactact gcgcaatagg 600atccatgtcg
cctattatag aaatttggga tttagatatt atcaatgtaa tcgaacctag
660ttttacgtta ggtcaagtaa agacgaagaa aagcaacaaa gttataggac
acacagatgc 720agttttagca ttggcttgga acaaaacatt cgaccatgta
atagcaagtg gatcagtaga 780ccaaacaatt catctatggg atatggaaat
taaaaaaccc agtacaacca ttaaatcgtt 840tacggataaa gtacaatgtt
tggaatggca cgctttagaa gctcaaacgc ttttagcagg 900aggttgcgat
agttctataa gagtatttga ctgtagaact ccagaagctc atcaaacttg
960gctcttagat ggcgaagctg aacggatatg ttggaatcca ttggaacctt
ttagtttttt 1020ggctggtacc agtaaaggtt cagtccaatg tttcgactgc
agaaaagggg aactgtggtc 1080tatttcagct cacagtaaag aagttagtgg
cctatgtgcc agtacacaat gcccaggtct 1140actaataacg tcttctactg
atgaaacagt gaaaatatgg gattataaag acctagcaaa 1200tgctccttgc
ctaataaatg aaaaggaatt taacttgggt aatgtgcact gcttagatct
1260ttgccctgat ttacccttcg ttatatcagc tggtggagat aaaaagtcgc
ataattttac 1320agtgtttgat atacagaata ttgatgttgt tagaaacaca
tttcaaccga gaggcttaat 1380taaatcagaa tctgttaaag aagaaggtgc
ttctacctca acttagagat atgcac 14362201420DNAPhyllotreta cruciferae
220aattaacctc aatttcaaac caaaaacact acacgtgaaa gaaaacaagt
atttcaattt 60cacgatggaa atagacgcac agacagaatc aaacataaat ttcataccat
gtataaaatg 120ggtaagaaaa ggcatagcta atacgaatcc cgtgaaactc
caactgtcga aaaacgagct 180agtcgatata ataaatgaaa cgaaagtgaa
gctccaagaa gctgaagaaa acgatgatga 240aattatggaa ggcgatgagc
cgaggcaaga tgaattcaat ctagaacagt acgatgaaga 300tgaaggcgac
gagaacactg cgaatgcttt aggcataggc tcgatagctg aattggacaa
360tgcagccgag gataatttct cagagtctga cgattctgat aaagaagatg
aagtcattaa 420aaccaccgat aatctactat tggtggcgca tgtagaagga
gatgcgagca ttttggaggt 480gtacgtttac aatgaagaag aagaatcttt
gtatgtacat cacgacatta tcttaccatc 540gtttccactt tgtctagaac
acttgaatta tgaacccaaa atgcctaagg gtaactactg 600tgcaatagga
tccatgtcac ctattataga aatttgggat ttagatatta tcaatgtaat
660tgagcctagt tttaccttag gtcaagtaag gactaagaaa agcaacaaat
ctatagggca 720cacagatgca gttttagcat tagcttggaa caaaacattc
gaccatataa tggcaagtgg 780atcagtagac caaacaattc atctatggga
catggaaatt aaaaaaccca gcacgaccat 840caagtcgttt acagataaag
tacaatgttt agaatggcac tcgttcgaag ctcagacgct 900tttagcagga
ggttgcgata attctataag agtatttgac tgtagaactc cagaagccca
960tcaaacgtgg ctcttagacg gtgaagctga gagaatatgt tggaatccgt
tagaaccttt 1020cagttttttg gctggtacca gcaaaggttc agttcaatgt
ttcgattgca ggaaaggaga 1080actgtggtct ctttcagctc acagtaaaga
agttactggc ctatgtgcta gcaaacaatg 1140cccaggtttg ctaataacat
cgtctactga tgaaacagtg aaaatatggg attataagga 1200cttaacaaat
gctccttgct taataaatga aaaggaattt aacttgggta atgttcactg
1260tttggatctt tgccctgatt taccctttgt tatatcagct ggtggtgaca
ataagtcgca 1320taattttaca gtatatgata tacagaatat tgatgttgtt
agaaacacat ttcaaccgag 1380gggcttaatt aaatcagaat ctgttaaaga
agaaggtgcc 14202211282DNALeptinotarsa decemlineata 221ttttttttaa
gtttcgtcaa aacatggaag aaaattcgca aagtaacata aattttgtac 60cctgtgttcg
ttgggtgaaa cgaggagtag caaatgcaaa tccagcaaag gttcagctat
120caaaaaatga gttgatacag ataataaatg atacaaaaaa caaactgaat
gtggctgata 180atgaagacga agtcatggaa ggagaaccgt caggaacaga
cgagttcaat tttgaaaatt 240atgatgaaaa tgacgaggct agcactgcaa
atgcacttgg gatcgcatcc ttagccgatt 300tacctaccgg agcagaagat
aatttctccg agtcagatga ttccgagaaa gaagatgatt 360taatcaagcc
tacagataat ctaattctag tgggacatgt tgaaggagat tccagtattc
420tggaagtgta cgtatacaat gaagatgaat gttctctgta tgtccatcac
gatatcgtct 480tgccgtcctt tcccttatgc cttgagtggc tgaactatga
gccaaacatg ccggcaggca 540attattgtgc agtaggtacg atgtctccaa
tcatagaagt gtgggacatg gatatgatga 600acgtaattga accatcgttt
actcttggaa aagcaggcag caagaagaaa aaccgcaaga 660ggataggtca
cactgatgct gtcctttctt tggcctggaa tcatacctac gatcatgtca
720tggcaagtgg atcagtagac aaaagcataa tcctgtggga catggaacag
aaagtgccca 780gcacaactat taacgttttt ggggacaagg tgcagtgcct
cgaatggcac aggatggaag 840cccaaactct tttggcaggt ggttgcgata
gcactgcaag agtctttgac tgccgaactc 900cagaacctca tcaaatttgg
caattagatg gagaagcaga aaggcttatg tggaatcctt 960tggagcctta
cgtgtttcta gctggtacca gcaacggctc tatccaaagc ttcgattgta
1020gaaaaggtca actttggtca attaatgctc attctaaaga agtaactggt
ctggcgatca 1080gtcgtcagtg tccgggtctt ttggtgaccg cttctcccga
tgaaatggtc aagacttggg 1140attttgcatc aggaagcgtg ccgaagcttg
tacacgaaag ggagttcaaa ctgggtaaca 1200tccactgttt ggaactttgc
cccgattccc cattcgttat aactctagga ggggataata 1260agtcgaacaa
tttcgccgtt tt 12822221353DNATribolium castaneum 222atggaagaaa
acgacaccga aaatcgagtt aatttcatca cttgcgtcaa gtgggtgaag 60aaaggagtgg
caaaaagcca acccgaaaag gtccaattgt ccaaagccga gctcgtacaa
120gtcataaaag ccacaaaaaa gaaacttcga gtcacaaatg aggaaaatgc
tcaaggtgaa 180tcatcggctc aagatgagtt caatttggaa aattatgacg
aacaagacga aggcaccgct 240gaggctttgg ggataagttc actggctgat
ttacaaaata atgaggaaga aaacttctct 300gaatcagacg attcggacaa
agaagacgaa gttatcaaac ctaacgataa tttaattcta 360gttggacatg
tggaaggtga cgcaagttcg ctagaagtgt acatttacaa cgaggaggaa
420gaatcacttt acgtccacca cgatatctta cttcccgcct ttccactttg
tttcgaatgg 480ttggactacg aacccaacgc cccaaaaggc agttactgtg
ctataggttc catgaccccc 540attatccaag tctgggactt ggacataatt
aactgcatag aacccgcgtt taatttaggc 600cgagccgcaa gtattaaaaa
aaaccgacct cacgtcggcc acaaagacgc agttttaact 660ttggcttgga
acaaaaccta cgaacacgtc ttagctagcg gttcagttga caaaactata
720cttttgtggg acttggagaa caaaactcca agtacgacca tttccgcgtt
caaagacaag 780gtccagtgca tgcagtggca caaactggaa gcgcaaactt
tactagctgg gtctagcgac 840aagaaagcaa aaattttcga ttgtcgcaac
cctgagacac atcagacgtg gaaaataaat 900ggggaggttg aaactctggt
ctggaacccg ctacaaccgt tttcattttt cgccggtagt 960gataccggaa
atttgcaata ttttgattgt agaaaaggta gtcaagtgtg ggctgttgaa
1020gcccatgaaa aggaagtcac tgggttggtt gtgagtccgc aatgtcccgg
tttattagtc 1080acatcgtcgc cagatgggac gataaaaatt tgggactaca
ccgaaaatga agccactttc 1140gtgttcgaaa aagattttag tttagggaca
gtccagtgcc tggatttgtc gcccgatttg 1200ccgtttgtca tagcggcagg
aggggataat aaatctaata attttctagt tcacgatttg 1260agaaacatcg
atgttgtaaa acacaagttt ggagaccggc aattagaaga attagtcgca
1320gaaagtacag aagaaagcat gaacactgat tag 13532231435DNAEpilachna
varivestis 223tataactaaa agtagcaata ttgactttga gaattataag
gcattgcata aataaataag 60aggtgatatc aatggaacac gagttggaag aagaattgga
atcgcagagt agaattaatt 120ttataccttg tttaaaatgg gttaaaaaag
gtgttgccaa cacaaatcct atgaaggttc 180aactctcaaa gaatgaattg
gtaaaaatta tcaaagatac acaaaaaaag ctagtccttg 240ccaaaaatga
atcaaataga gatggcgacg aagaagcagt tgatgacctc aatctaagtg
300attatgacaa tgaagatgaa aacactgcta atatacttgg catcggttct
ttggcggagt 360taacaaatga tgcacaagat aatttctcag aatctgatgg
ttctgatata gaagatgatt 420taatacttgt gggacatctt gagggtgata
caagcatttt agaagtttat gtctacaatg 480aggaagaaga gtccttatat
gttcatcatg atatattatt accaagtttt ccattatgct 540tagaatggct
agactatgaa acaaattccc ctaaaggcag ctattgtgct ataggttcca
600tggatcctat aatagaagta tgggatttag atataatcaa ctcagtggaa
tcagctttta 660aattaggaca aatgggtagt cgaaagaagg gtcgacccca
tataggacac agggatgcag 720tattatcttt agcatggaat aggtcattcg
agcatatatt agctagtggt tcggcagata 780aaagtgtaat attgtgggat
ttggatagaa aagaacctag tactacaatc aaagcatttg 840aagataaagt
acagtgtcta gattggcata aattagaggc acaatcttta ttagcaggtg
900cttgtgatgg tcgtgtaaaa gtctttgatt gccgaactcc tgatagtcat
ttgacttgga 960atttagatgg tgaatgtgaa cagctctcct ggaatcccct
acagccattt actttcattg 1020caggcacaag tgtaggttct ttgcaatgct
ttgactgtag aaaaggtcag ttatggtcga 1080ttccagctca tgaaaaggaa
gtcaccggac tctttgtgag tggtcaatgc ccaggattaa 1140ttgtcacatc
ttctccagat gaaacagtga aaatctggga ctatagtggg gaaaattctc
1200ctgaattgat tcacactaat gactttaatt gtggaatgtt acattgcttg
gggggttctc 1260cagattctcc atttgtaata gcttcaggtg gagataagaa
gtccaataat ttcttagttt 1320acgatatacg aaatatagat gctgtaaaac
acaggtttga gggcagggaa ttagtacagc 1380tggttcctac cactccatca
gaaactccag aggtcatgca acaagatgat taatg 14352241432DNAVibidia
duodecimguttata 224gtatcctgtg taaaatggat agaaaaagga aaagctcgtg
cagaaccttt gaaggttaaa 60atttcgaagg aagaactggt agaaatgatt aaaaatacta
aggaacaact cagtctcagt 120aacttagaaa atcaagaaaa tgaaccatca
gatgacgttg atatggagaa tggagatggt 180aataatgtgg ggttagatct
catcgatgac ttatccacga atcctgaaga ttatttgagt 240gaatctgaag
aagaatccga taaagaggat gacatgataa aaaccagtga taatttggta
300cttatcggta aatttgatgg tgacgcttgt tcgttagagg tccacgttta
taacgacgaa 360gaggtttcgt tttattgtca ccacgatttc ttattgacaa
gttttccggt ttgtttcgaa 420tggttgaatt acgaggcgaa cagtccgttg
ggtaactacg ttgcagtcgg tatgatggaa 480ccggttatcg aggtttataa
tttggacgtt ataaacgtta tgacgccttc gtacgaactt 540ggacgaaaag
ctaacaggag gaaaaattta cctcaaatcg gtcataaaag ttcagttctt
600tccctggctt ggagtagagc gtacgaacac gttctagcta gcggttcttc
cgataaaagt 660attattctgt gggatttgga aaagagggag ccgagtacta
cgattaaatc gttcgaagga 720gacgttcagt gtctcgagtg gcataaagtg
gagacgcaat ctttattagc gggtgcatgt 780gatagtcaag tgaaactttt
cgattgtaac agtccgagta gtcatttaac ttggaaatta 840gacggagaat
gcgaaacttt gaattggcat ccgcatgagc cgttcacgtt cttagcaggc
900accaatgttg ggtctttaca atgtttcgac tgcagaaaag gtctgatctg
gtcgttggct 960gctcacgaga aagaaatcag tggggttttt atcagtagcg
aatgcccggg attaatgatt 1020acgggatctc atgacggcat ggttaaaacg
tgggattaca aaggggagga tgtacctgaa 1080ttggtctatt ccaaagactt
caatttagga acaattttat cccttgaagg ttcaccaaac 1140tcacccttcg
taatagcagc aggtggcgac aacaaagata ataattttgt aatatacgac
1200gttcgtaaca ttgacgttgt gaaacacaag ttcgagaata gggaattgaa
aaaattggaa 1260acttccacac ctgccgagca gacatgaaga gtaattaaaa
tgtaattaaa ggagaatgga 1320atggatataa gagggagtga atgtcattat
gatgggcttt catgaaagta gattgagaag 1380tgaaaattta tatttgttat
attttgaata aatatagtta atttaaaaaa aa 14322251470DNAPectinophora
gossypiella 225aaaactcaaa agagtgaagt gagcacgtga aatcaagaaa
attcgtgaat ttgcgcgttg 60tttcctccat aataatggaa gaaaattcgc ccacggtcag
cttgatttca tgcatgcatt 120ttgtacgccg tggcgtagcg aaatcggtgc
cagaaaagat tgagcttaca gaaaaagagt 180tagagagcat aattaagcaa
actgctgaag atttacggct tactgagaat cctgaggaca 240gcgaggatga
ggaaggagaa gcgggtgcta gtgctgtcag agatccacca gcaaatccta
300atgatgagtt tgattttgaa aaatatgacg aggaagatgc aagtgttaac
ccaatcggga 360ttggctcagt ggctacccta cctaatttag gtgatttgag
tgaaaatgtc caaatcagga 420ctgaagggcc agatagtgac gaagaagatg
acataatcaa gccagatgac aacttgcttc 480tggtcggcca tgttgagagt
gatgctagca tactagaggt atacatattc aacaaagaag 540agggttcatt
ctatgtgcac catgacgtta ttctgccttg gttcccgtta tgcattgaat
600ggctgagtca tgatccatct gatcctaatc caggtaatct ttgcgctctt
ggtggtatgg 660acccagtcat ccaagtatgg gacttggata ttgaaaactg
tctagaacca gcgttcaaac 720tcgggaaaaa gccgaataaa aagaaaaaga
taaaacgggt tggacacaaa gacgctgtat 780tagatctatc ttggaatagg
aacttcacac atgtactagc aagtggttca gcagacaata 840cagttttgct
atgggacctg gatcaagggg cacctcacac gacactcagc tactttgaag
900ataaagtaca atcgctaacc ttccatccat tagaagctca aacattacta
tctgggagtt 960gcgacgggcg cgcacgagtg accgactgcc ggacaccaga
cgcacatcgc gcctggcaac 1020ttgcagggga gatagagagg gtcacgtggg
acaaacagaa tcccttctgt tttgcaatga 1080gcaacaacga aggcaaggtg
tcgtacgtag actgcagaca agacgaacct ctatggacca 1140taactgcaca
tgaaaaagaa gtcacaggtc ttattctcag tgaacaagtg ccagggctaa
1200tggtcacaat aagcacagat ggcaaacaga agacttggga tatatccaac
gctccaccag 1260tccaggtgag cgagcgaagc ggccgcgtcg gccaagcgtt
gtgcgcggcc agctgcccag 1320acgcaccctt ctccatagcc gtcggcggcg
acaacaagga atgctgcatc gagatggtgg 1380acctcaccat caacgaacaa
tttgtccacc ggttcgggtc gcgacccctc gtgcaagtca 1440ctacagaatc
aaatgacgac gcaatggaat 14702261361DNASpodoptera frugiperda
226acagtcagct taatatcatg tatgcacttt gtgcgtcgcg gagtggcgaa
agcagtgccg 60gaaaaaatag aattaacgga gaaagaattg gagaaaatta tcaaacaaac
agctgatgat 120ttgaggatag ctgaagctgg tgatgatgag agtgatgaag
aaggagaagc aagtgctgcc 180ccgagagacc caccagcaga tcctaatgac
gagttcaact ttgaaaagta tgacgaagaa 240gataatatta accctgtcgg
tattggtact gtagctacat taccaaattt gggtgactta 300agtgaagggg
tacagataag gacagaaggg ccagacagtg atgaagagga tgacatcatc
360aaaccaaatg ataatctgct tttagtcggc catgttgaga gcgacgccag
cgtcttagaa 420gtatacattt acaacaagga agaggattcg ttctacgtac
accatgatat cattctcccg 480tggttcccgc tgtgtattga gtggctcagc
catgacccct cggatcccaa tcctggcaac 540ctctgtgccc tcggtggtat
ggacccggtt atccaagtgt gggacttgga catcgagaac 600tgtctggagc
cagccttcaa gttgggtaaa aaacctaata aaaagaagaa aactaaacgg
660gtcggacaca aggatgctgt tctagatcta tcgtggaaca ggaactttac
acatgtgcta 720gcgagtggat cagctgacaa cacagtgtta ttatgggacc
tggatcaagg cacgccacac 780accaaaatcg actgcttcca ggataaggta
caatccctag cattccaccc gctggaagcg 840cagacgttgg tgagtggttc
gtgcgacggg tacgcgcgcg tgtcggactg ccgcgcgccc 900gacgcgcacc
gcgcatggaa cctcgggccc gagatagagc gggtcgtctg gaactcacag
960aatccgttct gttttgctat gagtaacaac cagggtaagg tagcgtacgt
ggactgccgg 1020aacgacgcgc cgctgtggac ggtcgacgcg cacgagaagg
aggtcaccgg cctcatactc 1080agcgaccgag tgcccggcct catggtcacc
gtcagcaccg acgagaaact caagacttgg 1140gacatttctg gtgcatcacc
gcaacaagta agcgagcgca catgtcgcgt gggccaggct 1200ctgtgcgccg
cgctgtgtcc ggacgcggcg tacagcgtcg ccgtcggcgg ggacaacaaa
1260cagaactaca tcgaactcgt cgacctcacc attagtgaac aatttgaaag
tcggttcgca 1320tcgcgacccc tgctcatacc acccactgcg cctagtgaag c
13612271482DNAOstrinia nubilalis 227ctcgtgtttc tcagcagaat
cgcatgtttt tttgctagta ttttagattt tatttataag 60tgaattacat aaaaatggaa
gaaaacacac ctacagttag cttagtatca tgtatgcatt 120ttgttaggcg
aggtgtggct aaagcggttc ccgaaaagat tgaactaacg gaaaaagaat
180tagagaaaat aatcaaacag accgcaaatg atttaagatt aacggaagcc
ggagacgatg 240tcagcgacga agaaggcgaa agttctcgcg gcccaccaga
cccgcccgct aatcccaatg 300atgaatttga ctttgaacac tatgaccaag
acgacacaag taatccagtt ggtattggct 360cagtggcaac attacccaat
ttaggtgact taagtgaaaa tgttcaaatt aggacggaag 420gaccagacag
tgatgaggag gatgacatca tcaaagcaga cgataacctg cttctagttg
480gtcacgttga aagcgatgcc agcgtactgg aagtatacat tttcaacaaa
gaggaagact 540cgttttatgt ccaccacgac atcattcttc catggttccc
actgtgcatt gaatggctga 600gccatgaccc ttcagaccct aacccaggca
acctctgtgc cctcgggggc atggacccag 660tgatccaggt gtgggacctc
gacatcgaga actgtttgga gccggccttc aagctcggca 720agaagcccaa
caagaagaaa aagacgaagc gcgtgggaca caaggacgct gtgcttgatc
780tgtcttggaa taggaatttt tcacatgtgc tggcaagtgg gtctgcagac
aacacagtgc 840tcctctggga cctggaccaa gggtcgccgc acacaaaact
aaactacttc gaagataagg 900tgcaatcggt gtcattccac cctctagaag
cgcagacgtt gttgacgggc gcgtgcgacg 960ggcgcgcgcg cgtcaccgac
tgccggacgc cggacgcgcg ccgcgcctgg cagctgccgc 1020cggagatcga
gcgcgccgtg tgggacacgc agaacccctt ctgcttcgcc atgagcaaca
1080accaaggaaa agtagcgtac gtggattgta gacaggatga gccgctatgg
accatagatg 1140cacacgaaaa agaaatcaca ggtctaatac taagcgacaa
agttcccggc ttgatggtca 1200ctgtcagtac agatggaaaa atgaaatcgt
gggacataac tagcggcgca tgcgtgcaag 1260tgagcgagcg ttcgggccgc
gtgggacagg cgttatgcgc tgcggcgtgt cccgacgcgc 1320ccttctctct
ggctgtgggc ggggacaata aggagtgctt catagaggtc gtcgacctgc
1380tggccagcga cacagtttca aatcgcttcg ggtcgcggcc gctggtgtct
ataacggtgg 1440aatcaaacga taacgctatg gaagcataga atataatttt gt
14822281604DNAManduca sexta 228atagaaaaaa gtcataaatc tgtaacttcc
aaacttagtc acctatgatt atactgtctg 60tgcatcattc attcaatcat tgttctagtt
ttaaatttgt ttatggtctt tatcagagtc 120tttatcattt gtcaatgtta
tgttaacgtg ttttgtctgg gttatgtgca tgtgtttata 180aaataaatat
atacaaatta aactagaaaa tggaagaaaa taccccaaca gtaagcttag
240tatcatgcat gcgttttgtt cgccgtggta tagctaaagc tgtaccagaa
aagattgaat 300tgaccgagaa agaattggaa aagattatta aacagactgc
tagtgaatta agaatttcag 360aacaggggga tgacaatagc gacgaagaag
gtgaagaagc tgcaagtgcg cctccagatc 420ctcccgcaga ccctaatgat
gaattcaatt ttgaacagta tgatgaagaa gatacaaata 480caaatcccat
tggaattggg actgtggcca caatgcccaa tttaggagac ctcagtgaac
540atgtgcagat cagaactgaa ggcccagaca gtgatgaaga agatgacatt
atcaagccta 600cagacaacct gttgctagtg ggacatgttg agagtgatgc
cagcatactc gaagtataca 660ttttcaacaa ggaagaagga tcattttatg
tccatcatga tatcattctg ccctggttcc 720ctctatgtat cgagtggttg
agccatgacc cttcagaccc aaatcctggc aacctttgtg 780ctctcggcgg
aatggaccca gtgatacaag tgtgggattt agatatagaa aattgtctgg
840agccagcttt caaactcggg aaaaaaccga acaaaaagaa aaaaacgaaa
agagtaggcc 900ataaagacgc agtgcttgac ttatcatgga atagaaactt
cacacatgtg ttagcaagtg 960gatcggcaga caacaccgtg ttattatggg
atctggacca aggcactcca cacaccaaac 1020ttgactactt ccaagacaag
gtgcaatcgg tgtcgttcca cccgctggag gcgcagacgc 1080tgctgtcggg
cgcgtgcgac ggctcggcgc gcgtgacgga ctgccgcgcc gccgccgcgc
1140accgcgcctg gccgctcggg cccgagatcg agcgcgccgt gtgggacacg
cacaacccat 1200tctgcttcgt gatgagcaac aacgagggta aggtggcata
tgtcgactgc aggcaaaacg 1260aaccgctatg gacaatgaca gcacacgaaa
aagaagtaac aggtctctta ttaagtgaac 1320atatacctgg tctaatggtt
accgtcagca cagacggcaa aatgaaaact tgggatataa 1380cagggtctgg
cgctactcag gtgagcgagc gcgcgggccg cgtgggccag gcgctgtgcg
1440cggcgctgtg tcccgacgcg ccgcactcgc tggccgtcgg cggcgacaac
aaggagaact 1500tcatcgaagt cgtcgacctc accattagcg aacagtattt
gaatcgtttc ggaagtcggc 1560agccagtatc catcactaca gaagacaact
ctatggaaac atag 16042291291DNAHelicoverpa zea 229ttttgatctc
atgtgcacat tgtttttgta catgtgatcg aagataaata catatattta 60agttataaat
tttgctatta caatggacga aacaacgcca acagttagtt taatatcatg
120catgcacttc gttagacggg gtgtggctaa agcagttcca gaaaagatag
agttaacgga 180gaaagagttg gagaagatca ttaaacagac tgctgatgat
ttgaggataa cagaggccgg 240tgaagatcag tcggatgatg aagacgaggc
tgctgcagcc cctagagatc caccggccga 300ccccaacgat gagttcaatt
ttgaaaaata cgacgaagag gatgatgtaa accctgttgg 360catcggtaca
gtggctactg taccaaactt aggtgatcta agtgaaaatg tacagataaa
420cacagaaggg cccgacagtg atgaagaaga tgacattatc aagcctaccg
acaacctgct 480tctggtcgga catgtagaga gtgatgccag tatcttggaa
gtatatattt ataacaagga 540agaggattca ttctatgtac accatgacat
catcctgcca tggttcccgc tgtgtgtcga 600atggctcagc catgacccaa
cagatcctaa tcctggcaac ctatgtgccc tcggcggcat 660ggacccagtc
atccaagtat gggacttgga catagagaac tgcttggagc cagcgttcaa
720gctcggcaag aaacctaata aaaagaagaa gaccaaacgg gttggacaca
aggatgctgt 780gcttgatatg tcttggaata ggaattttac acatgtgcta
gcaagtggat cagctgacaa 840cacagtcctc ttatgggacc tggatcaagg
cacaccacac actaaaataa aatgttttga 900agataaggta caatcagtat
cgttccaccc gttcgaggcg cagacgttgc tctcgggcgc 960ctgcgacggg
tcggcccgcg tgtcggactg ccgcagcccc gacgcacacc gagcgtggtc
1020gctggcgccc gagatagagc gggtcacttg gaacgtgcag aatcctttct
gttttgctat 1080gagcaacaac acaggcaagg tagcatacgt ggattgcaga
caggactcac ctctgtggac 1140actggatgct cacgagaagg aagtcacggg
actgatactg agcgagtcag tccccggact 1200catggtcaca ctcagcactg
atcagaagat gaagacgtgg gatatttctg gagcggcccc 1260ggtggcggtg
agcgagcgcg tggtgcgcgc g 12912302976DNAMegacopta cribraria
230ggttactttt gaaaaggtta tttatgtttg ttataaatat taggtccatt
tattcattac 60taaaaatatt ttttttatga aataccttgg aacttgaagt ttcttatcaa
attctttttt 120tggagggtgt ctccgaattt aatgtttctt atatggacat
aaagattatt ataaaatgga 180agtagaaact gaaagacgtg tccttgctaa
atttaagtct gatagggggg aggaaattgg 240tgcgttatat gacctaggaa
tttctgtcaa tgttgagcaa ttgacttaca tttgtaatag 300tctattggag
caggaggact ctgctcagta caccttttat gtgcatgatc aagaaatagt
360aaacacttta gagggtgtct tggacgtaca gaaactcaac actgaaaatg
ttgtcgaaat 420tatttatcag cagcaagctg tttttaaagt tagagccatt
accagatgta caagttctat 480gccaggacat gcagaagctg taatttcagt
caaattcagt cctaatagta gacatttagc 540aagtggatca ggtgatacca
ccgtaagatt ttgggattta actactcaaa cacctctgta 600cagttgtaaa
ggccattcta gctgggttct ttgcatctcc tggtcacctg attctaaaaa
660gctcatatca gggtgtaaga atggaattat tatattatgg gacccaatat
ctggaaaaca 720aaagggtcca ttaatgaaag gacataagca atggattaca
tcattagtct gggagccttt 780tcacttaaat gcagaatgca gatattttgc
cagtagctcg aaagatggtg atatcagaat 840atgggatgca cctgtgggaa
aatgtttacg agttctgact agtcattcaa aaagtgtaac 900atctttaatt
tggggagggt cttctcttct ctattctggc tcacaagatc gaacaattaa
960agtttggaga cctaacgatg gtgtcctctg tcgaacactg gaaggacatg
gacattgggt 1020taacagtctt tgtgtgaaca ctggctatgt tttaagtttg
ggatcatctt tcaggccatc 1080tgaaaatatt aacaaaaatg atgtcaaagc
attgcaaaat gctgctcttg aacggtacaa 1140agaagttatc ggttcagaag
gagagattct tgtgtctgga agtgatgatt ttactatgtt 1200tatgtggaag
cctgaaacaa ataaaaagtc ttttgaaaga ttgactggtc atcaacaact
1260gattaatgat gtaaaatttt ctcctgatac aaggattcta gcttcagctt
catttgataa 1320gtctataaag ctttgggatg gaaaaactgg gaaattcatt
accaccctac gtggacacgt 1380gcagtcagtt tatatgttgg catggtcttc
tgactcaagg ttgttagttt ctgcaagtgc 1440tgattcaact ttaaaagtat
ggaatatgaa aactaaaaaa cttctgaatg atctgccagg 1500acatgtagat
caagtttttg ctgtggattg gtctgctgat ggtgaaaagg tcgcatcagg
1560cggcaaagat aaactgttga aactatggca gtattaagaa tgtcttccag
gataacatag 1620gttcatagac ttcaagttct ctcaatgtat atatttttta
tatactttaa cttttagcta 1680cttttaaaat gttaatatat actattattg
agttttgttt attttttttt taaaaacgac 1740acaaatgttt tttagtcacc
caaggggttg gaaaaaactt ccaatcaaat atttatttta 1800tatacaatat
ctatacaaag ttgtctaata taaattatca gaaataaatt tgttatattt
1860tgacaatgta tcaaataaat gctggggctt tggtgccgat aggggatcga
caaactggac 1920agttttcttt catttcttct gagcagtctt cacacaaaca
aacatgacca catggtaaaa 1980taattatttc ttttgggttt tctctgcaag
caacacatag ttcgttttct gataactctc 2040ttctatctct agcatttcgt
ctcctttctc ttcttgtttc ttctagtctc ctcctttttt 2100cttcctcttt
cactttaagt tctttcataa cccaccattt tctggccaca atgactccta
2160gacctaatcc aatgctccca aaaagaagtg acaaccactt ataagtccct
tgttgctctt 2220ctaatttacg tattaaggaa gatatcggca tagtggtaag
ataatatgga tatcctgtcg 2280atggagggga tatttggaga ttaccactag
cattgataga aacttctcca acagcgatga 2340gaggagttcc ctctttcaac
aattcttctg ttgtttcaat accccgttgt cttactccat 2400taaaaaatcc
tattatgtgg tccataacac ttagtgtact attctcaaat tgattataga
2460ctgtgtccaa atccaataac tctgcagaaa caggatcaag gatttcaatt
gaaaatttac 2520ccctctttaa aacaaaaggt accacattat gcgatacatg
gataacattt ttctgatctg 2580cccaaaagcc agaagcattt cgagcaatag
catgttccat tatagtcttc ctcaaaagta 2640cgcctgagta atcatgagag
ccagaacttt taactgtttt accaactgct ctgacatctc 2700cttgatagat
tgtatatttc ttttcttcat cagtcaattt tttaatatca ggattcacac
2760caaggcgctg agcgcgttct aatagattaa tggaccaact gcaatttaaa
tacaaccttg 2820tgcatacccc cacaatgaca gcatcaatgc ctaatgctat
aagttcacct aggaaatcca 2880ttttataaat gcttgtaata ccttttttta
ttcattaaaa cctaagtatt atgatccata 2940aattttgtca ttcgttaatt
caaaacaaaa agtagg 29762313053DNANezara viridula 231gggggttagc
aaagcacgtg tttggagagt ataaatgctc atgtatttac ttagtttgaa 60gttgatgttt
atgaggtttt aaatcgaatt tgtaaaaaca cttctatttt aagtatttta
120aattaatgca atatcataat ttatgataga ttggttataa tgttatagat
tgattatcac 180ttttatcaaa cttagtaaat aagtttatat caataattta
taagttccat taaaatggaa 240gtagaaactg aggaaaagcg tgtacttgcc
aaatttaagt cagaccgcgg tgaagaagtt 300ggtgccttat atgaattaga
tatcaatgtt aatgttgatc agttgacata catttgtaat 360agcttactag
agcaggataa atctgcccaa tacacgtttt atgtcaatga acaagaaatt
420gttaagacct tagaaggtgt tctagatgtt cagaaattga atactgaaaa
tgtcgttgag 480attatttacc aacaacaagc aatttttaaa gttagagctg
ttacaagatg tacaagctct 540atgcctggtc atgcagaggc tgtcatttct
gtcaaattta gtcctaacag taagcatcta 600gcgagcggct caggtgatac
cacagttaga ttctgggatc tcacaacaca aacaccactg 660ttctgttgta
aaggccacaa caactgggtc ctttgcattg catggtcgcc tgactccaca
720aaattagttt ctggttgtaa ggatggccac atagttttat gggatcccgc
cactggcaag 780caaaaaggtc ctactatgaa aggtcacaag cagtggataa
cctcgctggc atgggaacca 840tatcacctta atgcagaatg cagatatttt
gccagcagtt caaaagatgg tgatatcagg 900atatgggatg caccgattgg
aaaatgcttg agaattctta ctagccattc gaaaagtgtc 960acttcactga
tttggggtgg atcttctctc ctttactctg gttctcaaga ccgtacaatc
1020aaagtttgga ggccgaaaga tggtatactt tgtcgtacct tggaaggtca
tgcacattgg 1080gtgaatagcc tgtcagtcaa cactgactat gttatgcgat
tgggttcatc tttcaggcca 1140tcagaaaatg tcaatcagaa tgataaggaa
gctttaaaga aagcagctgt tgagcgatac 1200cgtgaagtaa gtggttctga
aggagaaatt cttgtatctg gaagtgatga ttttactatg 1260tttatgtgga
aaccagaaac aggaaagaaa tcttttgctc ggttaacggg acatcaacaa
1320ctcataaatg atgtaaagtt ctcacctgat acaaggctac tcgcatctgc
atcatttgac 1380aagtctataa agctgtggga tggtaaaact ggaaaattta
taacaactct tcgaggtcat 1440gtgcagtcag tctatatgat agcgtggtct
gctgattctc ggttacttgt tagtgccagt 1500gctgattcaa cacttaaagt
atggactatg aaaacaaaaa aactattaaa tgatctccca 1560ggccatgttg
atcaaatatt tgctgtcgat tggtcggcag atggtgaaaa agtagcttct
1620ggtggcaaag ataaactttt gaaactatgg cagtattagt tggccaagaa
gactacactt 1680aaatgtaaat atgttttaag atgtatatac atgtatcgat
ggaagaaata ttttttaact 1740attaacaata aacaatactt cactccaatt
gtttttaaat accttacact ttgttccttt 1800caaagttctt ggtattaata
aaagaaatac aactatttaa tcgtctactg aacaaaatac 1860aatgccccta
aaatcgatgc cgaaataaaa tttataatat atatatattt tttttaattc
1920attcatcata taaaagctgc tgctttgctg acaataggcg atcgacagac
tggacaggta 1980tccttcactc cttcagaaca atcttcgcat aagcagacat
ggccacaatg taaaattatt 2040atttctttgg gattgaccct gcatgcaaca
cacaattcat tatctgatag ttcccttcca 2100tctcgcgcat ttcttctcct
ttccctcctt gtttcttcaa gcctcctttt tttttcttct 2160tcttttaacc
tcagttcttt cagagtccac cattttttaa cgacaattac tccaagaacg
2220actccaatgc ttccaaaaag gagtgtcaac cacctatatg tgctttgttg
ttcctctaat 2280tttcttatca gagatgacac tggcatggtg gttaagtaat
aagggtaacc agcagatgga 2340ggagacatct gaagataacc actgtgatta
accattactt ccccgatagc tgtcagtgga 2400gttccttctt tcaaaagttc
ttcagttgtt tcaatacctc tctgtcttac tccattgaaa 2460aaaccaataa
tatgatccat aacacttagc gtactgtttt caaactggtt atacactgta
2520tccaaatcta gtagctctgc agaaactgga tcaaggatct caatagtgaa
tttgcctttc 2580ttaagtacaa aaggtacaac attatgtgac acgtggataa
catttttttg atcagcccaa 2640aatccagatg cattacgtgc gattgcatgt
tccataatac tccttctcag gatcacacca 2700gaataatctt gtgagccaga
acttttaact gattttccca cagctttgac atctccttga 2760caaatagtgt
atttcttttc ctcatcgctt aaatttttta tttcaggatt gattccaagg
2820cgttgagctc tttctattaa attgatagac caactgcaat tcagatataa
cctagtacaa 2880acacccacaa ttatggtatc aatccctaaa gcgataagtt
cccccaagaa atccatcact 2940tttaattaca ttatttgagt ctcattttaa
ttatgaacaa accgataagt taaattttgt 3000cattgttttc aagttgatgc
agtaaggttc agcaaaaaca aagacatgaa aaa 3053232275DNADiabrotica
virgifera 232gctccaaagt atggtaccct agtgcctaac agaatattcg tgggaggaat
atcagctaac 60acaacagaag gagaattgat gcaacttttt agcaactatg gtactgttaa
agctgctaaa 120attatacagg acagggctgg tgtgtcaaaa ggatatggtt
tcatcacctt tgagagtgaa 180gatgatgcta aaaggcctct tagagaagct
gaaaacatag tgctaagaga aagaaaactt 240aatatagcac ccgctattaa
aaaacagcct tttag 275233275DNADiabrotica virgifera 233tgccagtcaa
ttcttttttc ctccgggtgc tgccgtgcca tattttcaga gtggagttac 60atattatact
cagccagctc ctgctgcacc tggagatccc actgctcaac aacctgttta
120tcaaccccct ccaatgtatc ccacgcaaac tggtcctcca caggcagcta
catacccttc 180tatgatgttt cctgctcaaa ctatatatat gccgcaacag
tatcccatgc ctattccgta 240cgagtacaac ttctaccagg gtaacggacc gtcgc
275234275DNADiabrotica virgifera 234ctcagtatct ggccggaggc
caaagcggtc cgggaaactc tcattgcacc tcattaccat 60catcaaacag tccacccaga
cccaattgtt atggtcaaca ggtgcctgcc tataattcag 120gagatcccgt
gtactacaac ttgccaatat acggagccac tttggaaggt ccgccattat
180acgctgatgc cttcgatcta agcgctagtg gagcttatgc ggaggaaacc
tacagcggca 240tgattactca agaaaatgta gaagcttctc ttgga
275235275DNADiabrotica virgifera 235cggtaaattc aactcgtgca
acttccgata attttacgca acatatggat caatctctcg 60ttgagtctga ttcaaatatt
gttaatgttg gaggtaaacc accagcacat aaatcttcag 120agacaattgt
ttctactcct tcccagcctc aggaggagag aaactcacac acgcctattg
180tttcactact ttccatagat catcaacagg aaaaagacta ttcatcaatg
caaagcggtc 240gtagacggaa acttctgatt caaagcaatc aaaat
275236275DNADiabrotica virgifera 236gtacccatgt atcctacaaa
cggatacgtc aaccagtttg tcggatataa tcctcaacaa 60ccatccccac cttcattcgg
tccacctctc tataacaacg gctacggtta ttctgaatat 120cgtcgattta
acggataccc agatctcagg tcaaataata atagtaataa accgaggaga
180cgaatatacg aaaataggcg gtcgaacgat cattctagcc gttccagtct
tcgcacagac 240tcaaactcat cggctagttg tgttgacgaa aataa
275237275DNADiabrotica virgifera 237cgaagaaaaa acttttcgaa
ctactgttaa cactcctcca cctgccccat actcacctat
60gaccaatcat caattcaaat ttattaatta cactaacacg aaaaacgttt ataatcataa
120gtacgctcac acgtttataa tcataagtac gctaacaaca ataattatta
taatagaaca 180gctacacaca atgaccgatt taaacatacc gaacctttag
cgaataaaaa caataacagt 240tccatttacc cattaacaaa taatttccta aataa
275238373DNADiabrotica virgifera 238tagccatacc caaagtaatt
ctgattccct tccctcccaa aattcccagg tacatgtgac 60gactacgggt cagagcttcg
tcccggcggc tatccaagct cagacgaaac gcaacaagcg 120ttctttgaga
agaagcggtg ccagtgccgg aggcatcaac gaaatcggcg ccggtgacgc
180ccctttacca ggcgaagaat gcgaagatgt ctacaagaag ctggaaacgc
ttaagttgta 240acgaaaaagt ggtatctttt cttgtatatt tttttctatt
tatttaagac tgtacaataa 300tgtaaaatac tagaaattta atgtaaacta
aagaggaaaa atatataata catatttatt 360gaagatgaaa aaa
373239381DNADiabrotica virgifera 239cgtttagtag tacatagaga
aatcgctttt agttttaatc ttttctgtgt tggaatattg 60aacaaattct aatgattcgt
aaaataacac ttctgttaaa aaccaaaact gatagtttgt 120tcaaaaaaag
tacaattttt ctgggcttgc tactgaaata atgtgccata ctctaattgt
180ggaacaaatt gtaggtatat gttgaaagtg aacaaataac taatgcttac
aataatgtcg 240acatcaagtc aaaataatgg taaaaagagt tcaagcagct
ctattgttaa caccccttcc 300agtactccca ttaccaatct tgcaaatggt
agtagtagta cccaaggaac agcacatagt 360tcacctccac ctaataacaa t
381240271DNADiabrotica virgifera 240tgcccaaatc aaataaatct
agaaatgcgt tttactactt tatggaggat tttaaacaaa 60gacaaggatt tgcttgtaaa
tcgatgaagg atgtagcaga tgctgcaggt ccccattggg 120ctaggatgtc
taaagcagaa agaagacctt atgaagagag ggcaaacaaa gagaagggag
180gtttgagata tacttgggat ggtcaatgcg ttgaagatat agaaaggagg
aacaaagctg 240aggacaataa aattgctaag atgaaagatg a
271241275DNADiabrotica virgifera 241cagtgggaga gcgaatctgt
agtgtcagac tcctctacag ctactttcaa taatagcttt 60ccaggtctga aatcgaaatg
gacattaagc aattctcaac ctgtagaaac tgactcaatg 120agccaaatgt
caggtcagag ctatgcaagt gctatgggaa ctagaagaca gttttctagt
180gcaatgggaa atagaggatc tcaaagagag tcctctaaca attccaacaa
tggtgacact 240agtgattcga ataccgatta ttttaacagt caatc
275242500DNADiabrotica virgifera 242tatcgactgc accttgaaat
ttgccaaaga aaattgtcga ctccgagatg aaatgttctt 60cataatccat atcaatactt
tctgttacca tgccagtggt gatcggtatt atcccgctga 120aattgccatt
tcatgcttca gcttggagga tggcgttttg cctcaaaatg tgtttcataa
180aattattaag ccaggtagac taccactggg gtatgctagt gacgcaaaaa
agcaatctga 240agattcacat caactgccta tccccttaac tggtgacgac
gacgacaatg tggaagaagt 300ttatacagag atgaaagaat ttatattatc
taaaaccgga gatactaaga aattccctcc 360cttgtatgct aagaaagata
ttgttaaaat gttgcaacat gtgttagaca cttggtgcgc 420tgattttgat
gagccgccat tatttaaagt ttattattta caatatatgt ttcaagtatt
480aaaaaactcg gtagctaacg 500243350DNADiabrotica virgifera
243ataacgtatg gcccttatat tcaatggcag aaacggaact ggataaggat
ctttattccc 60atactgttgg aatatgttgt gaattccatt ctgttagtga agcttgtcaa
ttttgcagca 120agtcagtggc agtacgactt gcatacacaa tttgtgataa
ttgctgttcg ttcctagata 180ttcctataga gcctggaaga cacgtacctg
atcaagcttt tacagcatcc agtttgagct 240caagagcttc atcaaaagct
agtttgtata caggttccag taaatcttct aagaagcagt 300cagttcgtga
agatgacaaa gacactgttt tatcgttttc tagtgcctct 350244500DNADiabrotica
virgifera 244atttccggct cttggaaggg gaagaggtat ggccagaaat cgaagaaatt
gatcattttt 60gactgcaatg ggtcttgttc cacttcatga gattgttaaa tgagtatgta
actgattaca 120ttaggcgaca atattttttt tagtgtcaga ttatttgatg
taattgtcct tttgacaatt 180ctttaatcat tgatgttttc ttctattgtg
gtgtttttag atgtcatatt tttatgtatg 240taaattggtt ttttatctaa
gtatagaata ttacatttta gtttaataag ataccagtaa 300agtatattgg
tttaccagtc gcctaatgta aactagtata tacataccta tattattttt
360ctttaatagt tttaagtcaa ttttgataca gatcacgaga cagatatttt
ttccgtctag 420tccgaattgc gttccgtttc ttgggccttg tcgaattgcg
tcccaaatat tttgcttacc 480tggccaccga taacgtctag
500245500DNADiabrotica virgifera 245tccgaattgc gtttcatttc
ttaattagcc aacccaattg cggtcagaag atagcttaat 60aaatattagt taagcaggga
aatacaatca acgtaatatc aactaacagt ttatttttat 120tttagcaccg
aatttataac taacacattt aacgaaattg tatcttgaaa tttcacccct
180gttatactgg ttaattttct gttcgagaaa aatatatttg ctggaggtag
ctttatctac 240atttctagaa atattttgtt taacagaatt tatttttata
taagaatcgg tttgaatgcg 300ctttaatgtt tcaacaaaca aaaaaatatt
aggatgtgga gacaaacaat aatttttaaa 360ttttgaatga aatcactcac
aagaattagt tgttctatat atagatgcgc tattctttgc 420ccaaatttgt
ggaggaaatg tgtagtcctc ttcgatatac ctttcaacta gatagtttgc
480aaatgcgtca actcgatgat 500246500DNADiabrotica virgifera
246catctggttt ttcaggcata aaatcttcca caaaacaatc tccaacttgg
ctcggaggaa 60gatacattag gccaaaaata tgcttcagcc atttacctat ttctgagttc
tcgttcttat 120actcagaact caaccccata ttttgtactt tacgccacct
gtaacaagat tatagaataa 180attctttatt tcagaaagta cttgcattca
agaaaaagtt tgaaacaggt cagattttat 240aaaaaaaata catctttaaa
aaataatgag aatatttagg agccaatttt ttgacagagg 300ttatgacgac
atcttttttt tatgtcaaaa atttgaagat attttaccta caattcttgt
360tttaacttat atcaaatgct gttttttgta aaaaaaaatt gaaaaaagaa
attacagtga 420agtttgaaat aaacatattg aaatgtttag ttttacagca
aaaaaaatag gatgacgtca 480taactcaagt ttttcttaac
500247500DNADiabrotica virgifera 247aatctactat ttgccagtta
cattgccata cgtattactt accaagcttg agcaagatga 60agtcgataac caacaacatt
tgcttccgga aaaacttctc gtacgctgga gtggatacct 120aattcgtaat
caatcactat tttcgttgga tttaaattgc atcccaaatc ttgacatttt
180ttctttaata attcgaataa tgatttgtaa gtgaattttt gcttattagg
taacaaacaa 240aatgcaaccg gaacataagt gtcattaatc aatgtatgta
atgtaaacat ttaaaaaaaa 300attgtgcaat aatcgaatgt tccatccata
aataaagtgg aattgttaca cataaatgaa 360agattagaca ttcatgaaaa
aacaaaaata ttaaactgtt catcattaat caacaaaaac 420tgttcatttt
tgatagtaaa tatttcaatt tgttccaaaa attcttgaac atccttggct
480gattttggca attttggaac 500248322DNADiabrotica virgifera
248cgttttcaat cgcgtgcgat taatgttctt tttttatgta ttggacatct
tttgttgaca 60gagaggtaag tacttcgctt tgcttttcga tttccttgtg taacaacttc
gcaggtcgtt 120ccgcaatatc ttctgttgct tttcttttaa gagaattgtt
aactttctgg cgaattaata 180cttcttcagc aagctcatga ttgtgttcac
cactaacttt tgaaaacaca ttatttaatg 240tatatagttt tgctttacac
tttttgaagt tatacttctt taggcgcgat tgagtaatta 300tttattatta
atctgcgcgc at 322249272DNADiabrotica virgifera 249tggagtacga
aaatacacaa caaaatataa acttcgtacc gtgtgtaaga tgggttaaac 60gaggagtggc
caattcaagc ccagtaaaat tgcaactgtc gaaaaacgag ctggctcaaa
120ttattaatga caccaagatt aaattacaag aatccaatga aaatgaagat
gagcctatgg 180aagaaggtga aacgtctcaa acagatgagt ttgccttaga
ggattacgat aaagaagacg 240aaaatgagga cactgcaaat gctttaggaa tt
272250275DNADiabrotica virgifera 250tggaaatcaa aaaaccaagt
acaaccatta aatccttcca agaaaaagtt caatgtctcg 60aatggcatcc tttagaggca
caaacacttt taggtggagg ttgtgacaaa tctgcaagag 120tatttgatgg
caggacccct gaaacccacc aaacttggct acttgatgga gaagctgaaa
180ggctatgctg gaacccatta gaacctttca catttttagc aggtaccagc
agtgggtctg 240tacagtgttt cgactgtaga aaaggacagc tatgg
275251248DNADiabrotica virgifera 251caccggctta cgtaataatt
aatttattcc tttcatttgc accatactat atattattta 60ttgagtggac aaatgttaaa
aagtaagtct ggcagcgaac gtgttaatga aacctagtaa 120aaggctccaa
catttgacac ttcttcatgc aacatgttct gttctgtgtc aatctaacct
180aacctaacat tttcaaatac gaatatggaa acatttcacc acatcaacac
gtgaattaat 240attcgaaa 248252272DNADiabrotica virgifera
252tcctctgtgt ttagaaccgc taaactatga accgaagatg cccaaaggaa
attattgtgc 60agtgggatca atgtcacctg ttatagaggt ctgggatgta gatattatga
atgttattga 120accttcattt actttaggga gacctgctag taaaaagaag
aacaaggaac atataggcca 180tacggatgca gtgctttcat tagcttggaa
caaaaccttt gaacatgtgt tggctagtgg 240atctgtagat caaacaatac
acttgtggga ta 272253486DNADiabrotica virgifera 253tcagttaaag
cacacagcaa ggaagtaaca gggttagtcc ttagcaaaca atgccaagga 60ttgttgatta
cttcttctac agatgaaaca gttaaaattt gggacttggc tacactggaa
120gctgaaccta agcttgttaa tgaaaaggag tttaatatgg ggaatattca
ctgtttggat 180ttatgtccag acttgccgtt tgttatatct gtaggtggag
ataagaagtc aaataatttt 240actgtttttg atgtacagaa tattgatgtt
gttaaacaca catttgggcc aagaggattg 300gtacagctag ttccagatac
agaagaaaat actaatacgt tttattgttt aataaagttt 360ttattttatt
tatttgtaat aatttcatgc ttcttcttta agtaccgtgc ccaaatatta
420gtcattggta gattccttga caatttgccg atactgtttt cgattactga
acaattcatc 480ttctga 486254600DNADiabrotica virgifera 254ggggctttct
gatttttgac agcttctata gaagtttatc aagatgttga tgccaaaaaa 60gaatagagta
tgtatttacg aatacctctt caaagaggga gtcatggtag ctaaaaaaga
120ttaccatgcc ccaaaacacc tcgaactaga aactatccct aaccttcaag
taattaaggc 180tttacaatca cttaaatcaa aaggttacgt aaaggaacaa
ttcgcctgga ggcattatta 240ttggtatttg actaactctg gcatcgaata
cctccgcaca ttcttacact tacctggaga 300aattgtccca tctaccttga
aacgcccagc aaggacagaa accacccgtc ctagaccagc 360tgctctcaga
tctgagacat ctaaaccttc agaagaccgt gcaggataca gaaggactcc
420tggaggccct ggagctgaca agaaagctga tgttggtcca ggaactggag
atgttgagtt 480caggcaagga ttcggacgtg gacgggcacc acaataaatt
tattgataag ttaattttta 540taaattgatc agccaataaa aagtttggtt
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 600
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