Romain F. Laine
Study of viral infection and development of optical super-resolution microscopy
Started December, 2017; ended September, 2020
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Overview: I am interested in developing and applying advanced microscopy techniques to decipher functional and structural organisation of life at multiple scales. For this, I develop analytical and optical tools. You can find me on Twitter (@LaineBioImaging), in the optics lab tinkering with microscopes or on my computer developing image analyses.
Background and how I ended up coming to the LMCB? French but I have been living in the UK for long enough to have forgotten how real buttery pastries taste like. I did my PhD at Imperial College in biophotonics (throwing lasers and microscopes at cells and molecules !), then moved to Cambridge for PostDoc to develop super-resolution microscopy (throwing more lasers and more microscopes at cells and molecules !). I now joined Ricardo’s lab and I want to exploit my microscopy skills to study more complex biological systems with my microscopy skills (throwing some cells at my microscope!).
Reason(s) that made me get into science? The idea (and ideal) of discovery!
Where do I see myself in 5 years? Head of my own lab, working on fast 3D microscopy to understand viral replication at cellular and sub-cellular levels.
Any hobbies? Capoeira and cooking ! (a well-balanced system of input and output of calories).
Research themes with our lab
Resources
Collaborators
Buzz Baum
Bassam Hajj
Christophe Leterrier
Florian Jug
Guillaume Jacquemet
Isabel Llorente-García
Joe Grove
Johanna Ivaska
Jason Mercer
Loïc Royer
Mike Heilemann
Mariana Pinho
Mark Marsh
Seamus Holden
Publications with our group (see more on Google Scholar)
| Fast4DReg – fast registration of 4D microscopy datasets Joanna W Pylvänäinen, Romain F Laine, Sujan Ghimire, Gautier Follain, Ricardo Henriques, Guillaume Jacquemet Published in Journal of Cell Science, February 2023 (see publication) |
| DeepBacs for multi-task bacterial image analysis using open-source deep learning approaches Christoph Spahn, Estibaliz Gómez-de-Mariscal, Romain F Laine, Pedro M Pereira, Lucas von Chamier, Mia Conduit, Mariana G Pinho, Guillaume Jacquemet, Séamus Holden, Mike Heilemann, Ricardo Henriques Published in Nature Communications Biology, July 2022 (see publication) |
| High-fidelity 3D live-cell nanoscopy through data-driven enhanced super-resolution radial fluctuation Romain F Laine, Hannah S Heil, Simao Coelho, Jonathon Nixon-Abell, Angélique Jimenez, Tommaso Galgani, Aki Stubb, Gautier Follain, Siân Culley, Guillaume Jacquemet, Bassam Hajj, Christophe Leterrier, Ricardo Henriques Published in bioRxiv, April 2022 (see preprint) |
| Avoiding a replication crisis in deep-learning-based bioimage analysis Romain F Laine, Ignacio Arganda-Carreras, Ricardo Henriques, Guillaume Jacquemet Published in Nature methods, October 2021 (see publication) |
| Democratising deep learning for microscopy with ZeroCostDL4Mic Lucas von Chamier, Romain F Laine, Johanna Jukkala, Christoph Spahn, Daniel Krentzel, Elias Nehme, Martina Lerche, Sara Hernández-Pérez, Pieta K Mattila, Eleni Karinou, Séamus Holden, Ahmet Can Solak, Alexander Krull, Tim-Oliver Buchholz, Martin L Jones, Loïc A Royer, Christophe Leterrier, Yoav Shechtman, Florian Jug, Mike Heilemann, Guillaume Jacquemet, Ricardo Henriques Published in Nature communications, April 2021 (see publication) |
| Application of Super-Resolution and Advanced Quantitative Microscopy to the Spatio-Temporal Analysis of Influenza Virus Replication Emma Touizer, Christian Sieben, Ricardo Henriques, Mark Marsh, Romain F. Laine Published in Viruses, January 2021 (see publication) |
| Single-molecule super-resolution imaging of T-cell plasma membrane CD4 redistribution upon HIV-1 binding Yue Yuan, Caron Jacobs, Isabel Llorente Garcia, Pedro M Pereira, Scott P.Lawrence, Romain F Laine, Mark Marsh, Ricardo Henriques Published in Viruses, January 2021 (see publication) |
| An Introduction to Live-Cell Super-Resolution Imaging Siân Culley, Pedro M Pereira, Romain F Laine, Ricardo Henriques Published in Imaging from Cells to Animals In Vivo, November 2020 (see publication) |
| Fluctuation-Based Super-Resolution Traction Force Microscopy Aki Stubb, Romain F Laine, Camilo Guzmán, Ricardo Henriques, Guillaume Jacquemet, Johanna Ivaska Published in Nano Letters, March 2020 (see publication) |
| Artificial intelligence for microscopy: what you should know Lucas von Chamier, Romain F Laine, Ricardo Henriques Published in Biochemical Society Transactions, August 2019 (see publication) |
| Automating multimodal microscopy with NanoJ-Fluidics Pedro Almada, Pedro M Pereira, Siân Culley, Ghislaine Caillol, Fanny Boroni-Rueda, Christina L Dix, Guillaume Charras, Buzz Baum, Romain F Laine, Christophe Leterrier, Ricardo Henriques Published in Nature communications, March 2019 (see publication) |
| NanoJ: a high-performance open-source super-resolution microscopy toolbox Romain F Laine, Kalina L Tosheva, Nils Gustafsson, Robert DM Gray, Pedro Almada, David Albrecht, Gabriel T Risa, Fredrik Hurtig, Ann-Christin Lindås, Buzz Baum, Jason Mercer, Christophe Leterrier, Pedro M Pereira, Siân Culley, Ricardo Henriques Published in Journal of Physics D: Applied Physics, February 2019 (see publication) |